AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1258470


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1258470 MtrunA17_Chr4g0057641 85.714 140 4 1 1 124 1 140 6.91e-75 218
Msa1258470 MtrunA17_Chr4g0057661 83.571 140 7 1 1 124 1 140 6.55e-73 213
Msa1258470 MtrunA17_Chr4g0057651 82.857 140 8 1 1 124 1 140 1.34e-71 210
Msa1258470 MtrunA17_Chr3g0116151 63.571 140 35 1 1 124 1 140 1.21e-52 162
Msa1258470 MtrunA17_Chr3g0116141 64.234 137 33 1 4 124 3 139 3.32e-52 161
Msa1258470 MtrunA17_Chr6g0459231 50.000 134 51 1 6 123 8 141 3.59e-40 130
Msa1258470 MtrunA17_Chr7g0225921 49.630 135 51 2 6 123 6 140 9.03e-39 127
Msa1258470 MtrunA17_Chr8g0373691 48.092 131 51 1 10 123 57 187 1.47e-29 105
Msa1258470 MtrunA17_Chr3g0111371 38.060 134 64 2 6 123 5 135 5.13e-23 87.0
Msa1258470 MtrunA17_Chr8g0392951 35.556 135 71 1 6 124 24 158 1.54e-20 81.6
Msa1258470 MtrunA17_Chr5g0434951 35.075 134 71 1 6 123 4 137 4.17e-20 79.7
Msa1258470 MtrunA17_Chr8g0391691 38.636 132 64 2 6 120 36 167 9.14e-20 80.1
Msa1258470 MtrunA17_Chr1g0161211 37.778 135 67 2 6 123 46 180 1.49e-18 77.0
Msa1258470 MtrunA17_Chr4g0033601 34.058 138 72 3 6 124 5 142 1.36e-16 70.9
Msa1258470 MtrunA17_Chr1g0169581 29.197 137 78 2 6 123 4 140 1.52e-16 70.9
Msa1258470 MtrunA17_Chr6g0482731 33.333 135 73 2 6 123 26 160 2.89e-15 68.2
Msa1258470 MtrunA17_Chr1g0169621 27.778 162 73 3 6 123 4 165 8.31e-15 66.6
Msa1258470 MtrunA17_Chr7g0245911 33.333 150 74 4 1 124 42 191 1.12e-14 67.0
Msa1258470 MtrunA17_Chr4g0044961 32.090 134 74 2 6 123 57 189 1.07e-13 64.3
Msa1258470 MtrunA17_Chr7g0256701 29.932 147 73 5 4 124 71 213 6.54e-13 62.8
Msa1258470 MtrunA17_Chr6g0460211 32.143 140 69 4 6 123 12 147 1.47e-12 60.5
Msa1258470 MtrunA17_Chr5g0440931 32.143 140 69 4 6 123 12 147 1.51e-12 60.5
Msa1258470 MtrunA17_Chr7g0254731 31.429 140 70 4 6 123 12 147 1.74e-12 60.5
Msa1258470 MtrunA17_Chr3g0093271 32.143 140 69 4 6 123 63 198 3.84e-12 60.5
Msa1258470 MtrunA17_Chr3g0121191 31.034 145 53 2 6 123 93 217 4.71e-12 60.5
Msa1258470 MtrunA17_Chr1g0188171 32.143 140 70 3 5 123 11 146 9.35e-12 58.5
Msa1258470 MtrunA17_Chr5g0402331 29.630 135 78 2 6 123 48 182 1.90e-11 58.2
Msa1258470 MtrunA17_Chr7g0256171 30.435 138 77 3 6 124 5 142 5.53e-11 56.6
Msa1258470 MtrunA17_Chr5g0402361 29.630 135 78 2 6 123 220 354 5.78e-11 58.2
Msa1258470 MtrunA17_Chr1g0166281 31.618 136 76 2 5 123 11 146 8.68e-11 55.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1258470 AT1G76650.1 41.912 136 63 1 4 123 41 176 4.20e-28 101
Msa1258470 AT1G76650.3 41.912 136 63 1 4 123 41 176 4.20e-28 101
Msa1258470 AT5G42380.1 41.176 136 62 2 6 123 49 184 1.19e-24 93.2
Msa1258470 AT1G76650.2 40.441 136 55 2 4 123 41 166 2.87e-24 91.7
Msa1258470 AT1G76640.1 38.849 139 66 2 4 123 20 158 4.18e-24 90.9
Msa1258470 AT1G24620.1 40.741 135 63 2 6 123 37 171 4.39e-23 89.0
Msa1258470 AT3G50770.1 39.161 143 65 2 3 123 61 203 5.46e-23 89.4
Msa1258470 AT3G01830.1 36.765 136 69 3 6 124 11 146 1.13e-19 79.3
Msa1258470 AT1G73630.1 33.577 137 72 2 3 123 17 150 9.31e-19 77.4
Msa1258470 AT1G18210.2 33.824 136 77 1 1 123 18 153 1.67e-18 76.6
Msa1258470 AT1G18210.1 33.824 136 77 1 1 123 18 153 1.67e-18 76.6
Msa1258470 AT5G37770.1 30.935 139 75 2 6 123 17 155 1.37e-17 74.3
Msa1258470 AT5G37770.2 30.935 139 75 2 6 123 17 155 1.37e-17 74.3
Msa1258470 AT1G66400.1 29.927 137 77 2 6 123 15 151 1.62e-17 73.9
Msa1258470 AT2G15680.1 34.815 135 69 3 6 123 50 182 1.28e-14 67.0
Msa1258470 AT4G03290.1 33.333 141 72 3 6 124 5 145 7.86e-14 64.3
Msa1258470 AT5G17470.1 31.618 136 70 3 7 123 7 138 2.23e-13 62.8
Msa1258470 AT1G66410.1 32.624 141 67 4 6 123 12 147 8.21e-13 61.6
Msa1258470 AT5G37780.1 32.624 141 67 4 6 123 12 147 8.21e-13 61.6
Msa1258470 AT4G12860.1 30.935 139 73 2 6 123 5 141 8.61e-13 61.6
Msa1258470 AT3G59440.1 30.714 140 79 2 3 124 48 187 8.68e-13 62.4
Msa1258470 AT1G05990.1 32.374 139 74 3 6 124 5 143 8.83e-13 61.6
Msa1258470 AT5G21274.1 32.857 140 68 4 6 123 12 147 9.73e-13 61.2
Msa1258470 AT3G56800.1 32.143 140 69 4 6 123 12 147 1.50e-12 60.8
Msa1258470 AT2G41110.1 32.143 140 69 4 6 123 12 147 1.50e-12 60.8
Msa1258470 AT2G27030.1 32.143 140 69 4 6 123 12 147 1.50e-12 60.8
Msa1258470 AT3G22930.1 32.117 137 75 3 5 123 34 170 1.94e-12 61.2
Msa1258470 AT3G43810.1 32.143 140 69 4 6 123 12 147 2.02e-12 60.5
Msa1258470 AT2G27030.3 32.143 140 69 4 6 123 12 147 2.73e-12 60.8
Msa1258470 AT3G43810.2 32.143 140 69 4 6 123 12 147 3.59e-12 60.8
Msa1258470 AT3G07490.1 32.609 138 74 3 6 124 5 142 3.83e-12 59.7
Msa1258470 AT3G10190.1 28.571 140 82 3 3 124 67 206 7.97e-12 60.1
Msa1258470 AT2G36180.1 30.597 134 73 3 10 123 4 137 1.90e-11 57.8
Msa1258470 AT2G43290.1 29.371 143 77 3 6 124 65 207 1.93e-11 58.9
Msa1258470 AT4G14640.1 32.374 139 69 3 6 123 13 147 4.07e-11 57.0

Find 41 sgRNAs with CRISPR-Local

Find 52 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGCGTGTTAAGTAAATTTC+TGG 0.177720 8_2:-59663860 None:intergenic
GGAGGATTTAATAACGCTTA+TGG 0.268235 8_2:+59664316 Msa1258470:CDS
TTAGGCCTATAGCGGTTTAT+AGG 0.314273 8_2:-59663961 None:intergenic
TGAATTTAACTTTGTATAGT+TGG 0.323358 8_2:+59664054 Msa1258470:five_prime_UTR
CATCACTTTACATTCTTCAA+TGG 0.327118 8_2:-59664468 None:intergenic
GATGATTGGAAGGTTTGATT+TGG 0.354750 8_2:+59664487 Msa1258470:CDS
TTAATAACGCTTATGGAATC+CGG 0.394530 8_2:+59664323 Msa1258470:CDS
AGATTTCACCGAACGAGTTA+AGG 0.395728 8_2:+59664198 Msa1258470:CDS
AAGAAAAGTTGAAGGATTTG+AGG 0.418092 8_2:+59664354 Msa1258470:CDS
TAACTCCTATAAACCGCTAT+AGG 0.418927 8_2:+59663956 Msa1258470:five_prime_UTR
TGTGTTGGCTATGAAACAAT+TGG 0.419857 8_2:-59663917 None:intergenic
GGAAATTGCTATTGAGGCAT+TGG 0.422915 8_2:+59664265 Msa1258470:CDS
TGGTGATGGGCTATTGAGTT+TGG 0.423743 8_2:+59664295 Msa1258470:CDS
AGAAAAGTTGAAGGATTTGA+GGG 0.426919 8_2:+59664355 Msa1258470:CDS
AGGAGTCGAATGACGAAGAT+TGG 0.473202 8_2:+59664218 Msa1258470:CDS
ATTCGACTCCTTAACTCGTT+CGG 0.474759 8_2:-59664206 None:intergenic
GAAGAATGTAAAGTGATGAT+TGG 0.486801 8_2:+59664473 Msa1258470:CDS
ATCAATGAAATCAAACAGTT+AGG 0.489044 8_2:-59663979 None:intergenic
TAATAACGCTTATGGAATCC+GGG 0.494077 8_2:+59664324 Msa1258470:CDS
AGAAGTGGAAATTGCTATTG+AGG 0.499700 8_2:+59664259 Msa1258470:CDS
TATATATTGTTCGCAATCAA+AGG 0.500104 8_2:-59664108 None:intergenic
AATGTAAAGTGATGATTGGA+AGG 0.500397 8_2:+59664477 Msa1258470:CDS
AGAGTTTCAATTGAAAGAAG+TGG 0.502556 8_2:+59664244 Msa1258470:CDS
TGAAGAATTCAGAATCATGA+TGG 0.506151 8_2:+59664532 Msa1258470:CDS
AGGTTTGATTTGGATGGTGA+TGG 0.507936 8_2:+59664497 Msa1258470:CDS
ATTGGAAGGTTTGATTTGGA+TGG 0.510943 8_2:+59664491 Msa1258470:CDS
TGATGGGCTATTGAGTTTGG+AGG 0.520299 8_2:+59664298 Msa1258470:CDS
GTGTTGGCTATGAAACAATT+GGG 0.522846 8_2:-59663916 None:intergenic
GCATTGGATTCTGATGGTGA+TGG 0.540839 8_2:+59664281 Msa1258470:CDS
TACTTTGATGAAGACGGTGA+TGG 0.542295 8_2:+59664173 Msa1258470:CDS
GGGAGAAGAAGAAAAGTTGA+AGG 0.545380 8_2:+59664346 Msa1258470:CDS
CTAAGTTACTTTGATGAAGA+CGG 0.574980 8_2:+59664167 Msa1258470:CDS
AGTCGAATGACGAAGATTGG+CGG 0.582253 8_2:+59664221 Msa1258470:CDS
CATTGGATTCTGATGGTGAT+GGG 0.600002 8_2:+59664282 Msa1258470:CDS
AATTAACATGAGGAGTAACA+TGG 0.611175 8_2:+59664130 Msa1258470:exon
ATTGAGGCATTGGATTCTGA+TGG 0.616237 8_2:+59664275 Msa1258470:CDS
TCAAACAGTTAGGCCTATAG+CGG 0.631415 8_2:-59663969 None:intergenic
AATAACGCTTATGGAATCCG+GGG 0.638897 8_2:+59664325 Msa1258470:CDS
AACAATATATAATTAACATG+AGG 0.645774 8_2:+59664120 Msa1258470:exon
TTGGCTATGAAACAATTGGG+TGG 0.680772 8_2:-59663913 None:intergenic
ATAACGCTTATGGAATCCGG+GGG 0.690847 8_2:+59664326 Msa1258470:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACAATATATAATTAACATG+AGG + chr8_2:59664120-59664139 Msa1258470:exon 15.0%
!!! TGAATTTAACTTTGTATAGT+TGG + chr8_2:59664054-59664073 Msa1258470:five_prime_UTR 20.0%
!!! TTTGAAATGTATGATAATGA+AGG + chr8_2:59664383-59664402 Msa1258470:intron 20.0%
! ATCAATGAAATCAAACAGTT+AGG - chr8_2:59663982-59664001 None:intergenic 25.0%
! TATATATTGTTCGCAATCAA+AGG - chr8_2:59664111-59664130 None:intergenic 25.0%
AAGCGTGTTAAGTAAATTTC+TGG - chr8_2:59663863-59663882 None:intergenic 30.0%
AATTAACATGAGGAGTAACA+TGG + chr8_2:59664130-59664149 Msa1258470:exon 30.0%
! CTAAGTTACTTTGATGAAGA+CGG + chr8_2:59664167-59664186 Msa1258470:CDS 30.0%
AGAGTTTCAATTGAAAGAAG+TGG + chr8_2:59664244-59664263 Msa1258470:CDS 30.0%
TTAATAACGCTTATGGAATC+CGG + chr8_2:59664323-59664342 Msa1258470:CDS 30.0%
AAGAAAAGTTGAAGGATTTG+AGG + chr8_2:59664354-59664373 Msa1258470:CDS 30.0%
AGAAAAGTTGAAGGATTTGA+GGG + chr8_2:59664355-59664374 Msa1258470:CDS 30.0%
! ATGTATGATAATGAAGGATG+TGG + chr8_2:59664389-59664408 Msa1258470:intron 30.0%
! TGTATGATAATGAAGGATGT+GGG + chr8_2:59664390-59664409 Msa1258470:intron 30.0%
AAGAGGATGTTGAAAAAGTT+GGG + chr8_2:59664434-59664453 Msa1258470:intron 30.0%
CATCACTTTACATTCTTCAA+TGG - chr8_2:59664471-59664490 None:intergenic 30.0%
GAAGAATGTAAAGTGATGAT+TGG + chr8_2:59664473-59664492 Msa1258470:CDS 30.0%
AATGTAAAGTGATGATTGGA+AGG + chr8_2:59664477-59664496 Msa1258470:CDS 30.0%
TGAAGAATTCAGAATCATGA+TGG + chr8_2:59664532-59664551 Msa1258470:CDS 30.0%
!! GTGTTGGCTATGAAACAATT+GGG - chr8_2:59663919-59663938 None:intergenic 35.0%
!! TGTGTTGGCTATGAAACAAT+TGG - chr8_2:59663920-59663939 None:intergenic 35.0%
!!! GCACACACTTATTTTTGTGT+TGG - chr8_2:59663935-59663954 None:intergenic 35.0%
TAACTCCTATAAACCGCTAT+AGG + chr8_2:59663956-59663975 Msa1258470:five_prime_UTR 35.0%
! AGAAGTGGAAATTGCTATTG+AGG + chr8_2:59664259-59664278 Msa1258470:CDS 35.0%
GGAGGATTTAATAACGCTTA+TGG + chr8_2:59664316-59664335 Msa1258470:CDS 35.0%
TAATAACGCTTATGGAATCC+GGG + chr8_2:59664324-59664343 Msa1258470:CDS 35.0%
TAACACCAAAGAGTTTGAAG+AGG + chr8_2:59664417-59664436 Msa1258470:intron 35.0%
AACATCCTCTTCAAACTCTT+TGG - chr8_2:59664425-59664444 None:intergenic 35.0%
GAAGAGGATGTTGAAAAAGT+TGG + chr8_2:59664433-59664452 Msa1258470:intron 35.0%
AGAGGATGTTGAAAAAGTTG+GGG + chr8_2:59664435-59664454 Msa1258470:intron 35.0%
GATGATTGGAAGGTTTGATT+TGG + chr8_2:59664487-59664506 Msa1258470:CDS 35.0%
!! ATTGGAAGGTTTGATTTGGA+TGG + chr8_2:59664491-59664510 Msa1258470:CDS 35.0%
TTGGCTATGAAACAATTGGG+TGG - chr8_2:59663916-59663935 None:intergenic 40.0%
TTAGGCCTATAGCGGTTTAT+AGG - chr8_2:59663964-59663983 None:intergenic 40.0%
TCAAACAGTTAGGCCTATAG+CGG - chr8_2:59663972-59663991 None:intergenic 40.0%
TACTTTGATGAAGACGGTGA+TGG + chr8_2:59664173-59664192 Msa1258470:CDS 40.0%
AGATTTCACCGAACGAGTTA+AGG + chr8_2:59664198-59664217 Msa1258470:CDS 40.0%
ATTCGACTCCTTAACTCGTT+CGG - chr8_2:59664209-59664228 None:intergenic 40.0%
! GGAAATTGCTATTGAGGCAT+TGG + chr8_2:59664265-59664284 Msa1258470:CDS 40.0%
!! ATTGAGGCATTGGATTCTGA+TGG + chr8_2:59664275-59664294 Msa1258470:CDS 40.0%
! CATTGGATTCTGATGGTGAT+GGG + chr8_2:59664282-59664301 Msa1258470:CDS 40.0%
AATAACGCTTATGGAATCCG+GGG + chr8_2:59664325-59664344 Msa1258470:CDS 40.0%
GGGAGAAGAAGAAAAGTTGA+AGG + chr8_2:59664346-59664365 Msa1258470:CDS 40.0%
GAGGATGTTGAAAAAGTTGG+GGG + chr8_2:59664436-59664455 Msa1258470:intron 40.0%
!! AGGTTTGATTTGGATGGTGA+TGG + chr8_2:59664497-59664516 Msa1258470:CDS 40.0%
AGGAGTCGAATGACGAAGAT+TGG + chr8_2:59664218-59664237 Msa1258470:CDS 45.0%
AGTCGAATGACGAAGATTGG+CGG + chr8_2:59664221-59664240 Msa1258470:CDS 45.0%
! GCATTGGATTCTGATGGTGA+TGG + chr8_2:59664281-59664300 Msa1258470:CDS 45.0%
!! TGGTGATGGGCTATTGAGTT+TGG + chr8_2:59664295-59664314 Msa1258470:CDS 45.0%
!! TGATGGGCTATTGAGTTTGG+AGG + chr8_2:59664298-59664317 Msa1258470:CDS 45.0%
ATAACGCTTATGGAATCCGG+GGG + chr8_2:59664326-59664345 Msa1258470:CDS 45.0%
! CAACTTTTCTTCTTCTCCCC+CGG - chr8_2:59664345-59664364 None:intergenic 45.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 59663843 59664559 59663843 ID=Msa1258470;Name=Msa1258470
chr8_2 mRNA 59663843 59664559 59663843 ID=Msa1258470-mRNA-1;Parent=Msa1258470;Name=Msa1258470-mRNA-1;_AED=0.04;_eAED=0.04;_QI=294|0|0.5|1|0|0|2|0|124
chr8_2 exon 59663843 59664390 59663843 ID=Msa1258470-mRNA-1:exon:18540;Parent=Msa1258470-mRNA-1
chr8_2 exon 59664439 59664559 59664439 ID=Msa1258470-mRNA-1:exon:18541;Parent=Msa1258470-mRNA-1
chr8_2 five_prime_UTR 59663843 59664136 59663843 ID=Msa1258470-mRNA-1:five_prime_utr;Parent=Msa1258470-mRNA-1
chr8_2 CDS 59664137 59664390 59664137 ID=Msa1258470-mRNA-1:cds;Parent=Msa1258470-mRNA-1
chr8_2 CDS 59664439 59664559 59664439 ID=Msa1258470-mRNA-1:cds;Parent=Msa1258470-mRNA-1
Gene Sequence

>Msa1258470

ATGAGGAGTAACATGGAATTTGAGCGTGTACTAAGTTACTTTGATGAAGACGGTGATGGAAAGATTTCACCGAACGAGTTAAGGAGTCGAATGACGAAGATTGGCGGAGAGTTTCAATTGAAAGAAGTGGAAATTGCTATTGAGGCATTGGATTCTGATGGTGATGGGCTATTGAGTTTGGAGGATTTAATAACGCTTATGGAATCCGGGGGAGAAGAAGAAAAGTTGAAGGATTTGAGGGAAGCTTTTGAAATGATGTTGAAAAAGTTGGGGGATTCTAAGTCCATTGAAGAATGTAAAGTGATGATTGGAAGGTTTGATTTGGATGGTGATGGTGTGCTTAGTTTTGAAGAATTCAGAATCATGATGGAGTGA

Protein sequence

>Msa1258470

MRSNMEFERVLSYFDEDGDGKISPNELRSRMTKIGGEFQLKEVEIAIEALDSDGDGLLSLEDLITLMESGGEEEKLKDLREAFEMMLKKLGDSKSIEECKVMIGRFDLDGDGVLSFEEFRIMME*