AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0748660


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0748660 MtrunA17_Chr4g0065221 93.289 149 7 2 1 146 1 149 2.52e-100 284
Msa0748660 MtrunA17_Chr2g0305131 59.459 148 49 6 1 145 1 140 6.78e-48 151
Msa0748660 MtrunA17_Chr4g0059251 39.130 69 42 0 66 134 62 130 4.02e-12 60.1
Msa0748660 MtrunA17_Chr6g0451141 31.897 116 70 3 14 124 14 125 9.76e-11 56.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0748660 AT5G25240.1 39.024 123 56 6 17 127 11 126 1.25e-20 82.0
Msa0748660 AT5G14895.1 38.202 89 42 3 61 136 60 148 6.62e-12 60.5

Find 39 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTAAGTGGAAAACGTTTCTT+AGG 0.293276 5_2:+1916368 Msa0748660:CDS
CTTAGGAAAATTAGTGGGTA+TGG 0.339302 5_2:+1916385 Msa0748660:CDS
TATGCTCTTAATTTCAATAG+TGG 0.374345 5_2:+1916448 Msa0748660:CDS
TCATTCTGGCGACGTGCATC+TGG 0.387586 5_2:-1916535 None:intergenic
ATATATATCATGTTTCATTC+TGG 0.388657 5_2:-1916549 None:intergenic
AAACAACATGGCTGCCTATG+AGG 0.423463 5_2:+1916117 None:intergenic
CACCAATGGTCTTCCCTCAT+AGG 0.431252 5_2:-1916131 None:intergenic
ATGCTCTTAATTTCAATAGT+GGG 0.436223 5_2:+1916449 Msa0748660:CDS
AACGGGTATGGATGCGGGTG+CGG 0.443003 5_2:+1916190 Msa0748660:CDS
AAGACCATTGGTGATGGAAA+TGG 0.450625 5_2:+1916141 Msa0748660:CDS
GAAAATAGTGAAGGAAATAG+AGG 0.458494 5_2:+1916280 Msa0748660:CDS
TGGCGACGTGCATCTGGAAA+AGG 0.467598 5_2:-1916529 None:intergenic
CTTTCCATTTCCATCACCAA+TGG 0.472269 5_2:-1916145 None:intergenic
TTAGGAAAATTAGTGGGTAT+GGG 0.477370 5_2:+1916386 Msa0748660:CDS
GTGGCGTTGCCACGATTTCA+AGG 0.486009 5_2:-1916230 None:intergenic
TTCGAAGAGACCAACGGGTA+TGG 0.486097 5_2:+1916178 Msa0748660:CDS
GAGACTTCAGAAGTGATTGC+TGG 0.496587 5_2:+1916343 Msa0748660:CDS
GGTATGGATGCGGGTGCGGA+TGG 0.500992 5_2:+1916194 Msa0748660:CDS
AAGATTTCGAAGAGACCAAC+GGG 0.512584 5_2:+1916173 Msa0748660:CDS
GAGACCAACGGGTATGGATG+CGG 0.514745 5_2:+1916184 Msa0748660:CDS
AGACCAACGGGTATGGATGC+GGG 0.516345 5_2:+1916185 Msa0748660:CDS
AGGAGAAGAGACATGGATGA+TGG 0.526434 5_2:+1916300 Msa0748660:CDS
TGCTCTTAATTTCAATAGTG+GGG 0.526773 5_2:+1916450 Msa0748660:CDS
ACGTTTCTTAGGAAAATTAG+TGG 0.531357 5_2:+1916379 Msa0748660:CDS
GGACAAGTTGAGGAAGATGA+AGG 0.537471 5_2:+1916321 Msa0748660:CDS
AACAACATGGCTGCCTATGA+GGG 0.543567 5_2:+1916118 None:intergenic
TGGCAACGCCACGACGAAGA+AGG 0.555768 5_2:+1916241 Msa0748660:CDS
GAAATAGAGGAGAAGAGACA+TGG 0.562044 5_2:+1916293 Msa0748660:CDS
AAGTGATTGCTGGTCCTAAG+TGG 0.571200 5_2:+1916353 Msa0748660:CDS
AAATATTCACCTTGAAATCG+TGG 0.578098 5_2:+1916221 Msa0748660:CDS
CATGGATGATGGACAAGTTG+AGG 0.582027 5_2:+1916311 Msa0748660:CDS
CGTTTCTTAGGAAAATTAGT+GGG 0.584307 5_2:+1916380 Msa0748660:CDS
TGAGGGAAGACCATTGGTGA+TGG 0.585967 5_2:+1916135 Msa0748660:CDS
CTACTTAATGAAAATAGTGA+AGG 0.588080 5_2:+1916271 Msa0748660:CDS
TGCCTATGAGGGAAGACCAT+TGG 0.600498 5_2:+1916129 Msa0748660:CDS
CAAGATTTCGAAGAGACCAA+CGG 0.625236 5_2:+1916172 Msa0748660:CDS
GATGCTTACCTTCTTCGTCG+TGG 0.640185 5_2:-1916249 None:intergenic
AAAAGGATTAGAAAACCTTG+TGG 0.651946 5_2:-1916512 None:intergenic
GCACCCGCATCCATACCCGT+TGG 0.669123 5_2:-1916188 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! CTACTTAATGAAAATAGTGA+AGG + chr5_2:1916271-1916290 Msa0748660:CDS 25.0%
! TATGCTCTTAATTTCAATAG+TGG + chr5_2:1916448-1916467 Msa0748660:CDS 25.0%
! ATGCTCTTAATTTCAATAGT+GGG + chr5_2:1916449-1916468 Msa0748660:CDS 25.0%
AAATATTCACCTTGAAATCG+TGG + chr5_2:1916221-1916240 Msa0748660:CDS 30.0%
GAAAATAGTGAAGGAAATAG+AGG + chr5_2:1916280-1916299 Msa0748660:CDS 30.0%
ACGTTTCTTAGGAAAATTAG+TGG + chr5_2:1916379-1916398 Msa0748660:CDS 30.0%
CGTTTCTTAGGAAAATTAGT+GGG + chr5_2:1916380-1916399 Msa0748660:CDS 30.0%
! TTAGGAAAATTAGTGGGTAT+GGG + chr5_2:1916386-1916405 Msa0748660:CDS 30.0%
! ATGGGAAAAAACAAAAGCAT+AGG + chr5_2:1916404-1916423 Msa0748660:CDS 30.0%
TGCTCTTAATTTCAATAGTG+GGG + chr5_2:1916450-1916469 Msa0748660:CDS 30.0%
AAAAGGATTAGAAAACCTTG+TGG - chr5_2:1916515-1916534 None:intergenic 30.0%
! CTAAGAAACGTTTTCCACTT+AGG - chr5_2:1916370-1916389 None:intergenic 35.0%
CTAAGTGGAAAACGTTTCTT+AGG + chr5_2:1916368-1916387 Msa0748660:CDS 35.0%
! CTTAGGAAAATTAGTGGGTA+TGG + chr5_2:1916385-1916404 Msa0748660:CDS 35.0%
GAAAACCTTGTGGAAAAACT+TGG - chr5_2:1916505-1916524 None:intergenic 35.0%
!! AAGACCATTGGTGATGGAAA+TGG + chr5_2:1916141-1916160 Msa0748660:CDS 40.0%
CTTTCCATTTCCATCACCAA+TGG - chr5_2:1916148-1916167 None:intergenic 40.0%
CAAGATTTCGAAGAGACCAA+CGG + chr5_2:1916172-1916191 Msa0748660:CDS 40.0%
AAGATTTCGAAGAGACCAAC+GGG + chr5_2:1916173-1916192 Msa0748660:CDS 40.0%
GAAATAGAGGAGAAGAGACA+TGG + chr5_2:1916293-1916312 Msa0748660:CDS 40.0%
AACCTTGTGGAAAAACTTGG+AGG - chr5_2:1916502-1916521 None:intergenic 40.0%
AGGAGAAGAGACATGGATGA+TGG + chr5_2:1916300-1916319 Msa0748660:CDS 45.0%
!! CATGGATGATGGACAAGTTG+AGG + chr5_2:1916311-1916330 Msa0748660:CDS 45.0%
! GGACAAGTTGAGGAAGATGA+AGG + chr5_2:1916321-1916340 Msa0748660:CDS 45.0%
GAGACTTCAGAAGTGATTGC+TGG + chr5_2:1916343-1916362 Msa0748660:CDS 45.0%
AAGTGATTGCTGGTCCTAAG+TGG + chr5_2:1916353-1916372 Msa0748660:CDS 45.0%
! TGCCTCCAAGTTTTTCCACA+AGG + chr5_2:1916497-1916516 Msa0748660:CDS 45.0%
TGCCTATGAGGGAAGACCAT+TGG + chr5_2:1916129-1916148 Msa0748660:CDS 50.0%
CACCAATGGTCTTCCCTCAT+AGG - chr5_2:1916134-1916153 None:intergenic 50.0%
TGAGGGAAGACCATTGGTGA+TGG + chr5_2:1916135-1916154 Msa0748660:CDS 50.0%
TTCGAAGAGACCAACGGGTA+TGG + chr5_2:1916178-1916197 Msa0748660:CDS 50.0%
GATGCTTACCTTCTTCGTCG+TGG - chr5_2:1916252-1916271 None:intergenic 50.0%
GAGACCAACGGGTATGGATG+CGG + chr5_2:1916184-1916203 Msa0748660:CDS 55.0%
AGACCAACGGGTATGGATGC+GGG + chr5_2:1916185-1916204 Msa0748660:CDS 55.0%
GTGGCGTTGCCACGATTTCA+AGG - chr5_2:1916233-1916252 None:intergenic 55.0%
TGGCGACGTGCATCTGGAAA+AGG - chr5_2:1916532-1916551 None:intergenic 55.0%
TCATTCTGGCGACGTGCATC+TGG - chr5_2:1916538-1916557 None:intergenic 55.0%
AACGGGTATGGATGCGGGTG+CGG + chr5_2:1916190-1916209 Msa0748660:CDS 60.0%
TGGCAACGCCACGACGAAGA+AGG + chr5_2:1916241-1916260 Msa0748660:CDS 60.0%
GCACCCGCATCCATACCCGT+TGG - chr5_2:1916191-1916210 None:intergenic 65.0%
GGTATGGATGCGGGTGCGGA+TGG + chr5_2:1916194-1916213 Msa0748660:CDS 65.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 1916124 1916564 1916124 ID=Msa0748660;Name=Msa0748660
chr5_2 mRNA 1916124 1916564 1916124 ID=Msa0748660-mRNA-1;Parent=Msa0748660;Name=Msa0748660-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|146
chr5_2 exon 1916124 1916564 1916124 ID=Msa0748660-mRNA-1:exon:561;Parent=Msa0748660-mRNA-1
chr5_2 CDS 1916124 1916564 1916124 ID=Msa0748660-mRNA-1:cds;Parent=Msa0748660-mRNA-1
Gene Sequence

>Msa0748660

ATGGCTGCCTATGAGGGAAGACCATTGGTGATGGAAATGGAAAGCAAACAAGATTTCGAAGAGACCAACGGGTATGGATGCGGGTGCGGATGGTTACAAATATTCACCTTGAAATCGTGGCAACGCCACGACGAAGAAGGTAAGCATCTACTTAATGAAAATAGTGAAGGAAATAGAGGAGAAGAGACATGGATGATGGACAAGTTGAGGAAGATGAAGGAGACTTCAGAAGTGATTGCTGGTCCTAAGTGGAAAACGTTTCTTAGGAAAATTAGTGGGTATGGGAAAAAACAAAAGCATAGGTTTCAATATGATGCACATAGTTATGCTCTTAATTTCAATAGTGGGGCTCAAAGTGAAGATGAAGAATATTTGCCTCCAAGTTTTTCCACAAGGTTTTCTAATCCTTTTCCAGATGCACGTCGCCAGAATGAAACATGA

Protein sequence

>Msa0748660

MAAYEGRPLVMEMESKQDFEETNGYGCGCGWLQIFTLKSWQRHDEEGKHLLNENSEGNRGEETWMMDKLRKMKETSEVIAGPKWKTFLRKISGYGKKQKHRFQYDAHSYALNFNSGAQSEDEEYLPPSFSTRFSNPFPDARRQNET*