AlfalfaGEDB | Msa1258670

M. sativa cultivar zhongmu-4 / Msa1258670

KEGG: K13448
GO: GO:0000302 GO:0001101 GO:0003674 GO:0005488 GO:0005509 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006950 GO:0006979 GO:0008150 GO:0010035 GO:0010193 GO:0042221 GO:0042493 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0046677 GO:0046872 GO:0050896 GO:1901700
PFAM: PF13499.9

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1258670	XP_003608847.1	95.714	140	6	0	1	140	1	140	1.63e-87	261

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1258670	sp\|Q8RYJ8\|CML19_ORYSJ	54.225	142	61	2	3	140	4	145	2.82e-41	137

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1258670	G7JLF5	95.714	140	6	0	1	140	1	140	7.76e-88	261

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0071480	Msa1258670	0.835938	1.253533e-56	-8.615850e-47
Msa0078150	Msa1258670	0.813160	2.946152e-51	-8.615850e-47
Msa0119440	Msa1258670	0.842626	2.305248e-58	-8.615850e-47
Msa0748660	Msa1258670	0.861836	7.752279e-64	-8.615850e-47
Msa0162690	Msa1258670	0.824693	7.029468e-54	-8.615850e-47
Msa0228530	Msa1258670	0.927982	5.490383e-92	-8.615850e-47
Msa1238680	Msa1258670	0.806240	9.055330e-50	-8.615850e-47
Msa1258470	Msa1258670	0.917002	9.011193e-86	-8.615850e-47
Msa1258670	Msa1285200	0.809641	1.711645e-50	-8.615850e-47
Msa1258670	Msa1421900	0.806957	6.391083e-50	-8.615850e-47
Msa0380600	Msa1258670	0.804849	1.773376e-49	-8.615850e-47
Msa0430240	Msa1258670	0.805757	1.144383e-49	-8.615850e-47
Msa0437070	Msa1258670	0.816522	5.295738e-52	-8.615850e-47
Msa0472710	Msa1258670	0.806064	9.861899e-50	-8.615850e-47
Msa0472770	Msa1258670	0.800458	1.427572e-48	-8.615850e-47
Msa0482280	Msa1258670	0.987818	1.187376e-171	-8.615850e-47
Msa1063410	Msa1258670	0.800172	1.632936e-48	-8.615850e-47
Msa1078330	Msa1258670	0.815050	1.127442e-51	-8.615850e-47
Msa0259440	Msa1258670	0.828414	9.112658e-55	-8.615850e-47
Msa0259580	Msa1258670	0.825930	3.583999e-54	-8.615850e-47
Msa0305620	Msa1258670	0.842887	1.964394e-58	-8.615850e-47
Msa0358110	Msa1258670	0.804316	2.290750e-49	-8.615850e-47
Msa1108720	Msa1258670	0.810797	9.642228e-51	-8.615850e-47
Msa1123720	Msa1258670	0.810635	1.044803e-50	-8.615850e-47
Msa0860250	Msa1258670	0.805572	1.251497e-49	-8.615850e-47
Msa0968050	Msa1258670	0.826967	2.028074e-54	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1258670	MtrunA17_Chr4g0057641	95.714	140	6	0	1	140	1	140	1.50e-91	261
Msa1258670	MtrunA17_Chr4g0057661	95.000	140	7	0	1	140	1	140	8.56e-91	259
Msa1258670	MtrunA17_Chr4g0057651	94.286	140	8	0	1	140	1	140	2.02e-89	256
Msa1258670	MtrunA17_Chr3g0116151	73.571	140	37	0	1	140	1	140	7.86e-69	204
Msa1258670	MtrunA17_Chr3g0116141	74.453	137	35	0	4	140	3	139	2.51e-68	202
Msa1258670	MtrunA17_Chr6g0459231	59.701	134	54	0	6	139	8	141	8.67e-55	168
Msa1258670	MtrunA17_Chr7g0225921	57.037	135	57	1	6	139	6	140	8.81e-51	158
Msa1258670	MtrunA17_Chr8g0373691	54.135	133	56	2	10	139	57	187	6.22e-38	127
Msa1258670	MtrunA17_Chr3g0111371	42.537	134	74	1	6	139	5	135	1.36e-30	107
Msa1258670	MtrunA17_Chr8g0392951	39.259	135	82	0	6	140	24	158	1.06e-27	100
Msa1258670	MtrunA17_Chr8g0391691	43.182	132	74	1	6	136	36	167	1.51e-27	100
Msa1258670	MtrunA17_Chr5g0434951	38.806	134	82	0	6	139	4	137	5.33e-27	97.8
Msa1258670	MtrunA17_Chr4g0033601	39.130	138	81	2	6	140	5	142	1.54e-25	94.7
Msa1258670	MtrunA17_Chr1g0161211	40.741	135	79	1	6	139	46	180	1.32e-24	93.6
Msa1258670	MtrunA17_Chr7g0245911	38.000	150	83	3	1	140	42	191	1.50e-23	90.9
Msa1258670	MtrunA17_Chr1g0169581	34.752	141	81	2	6	139	4	140	1.79e-23	89.0
Msa1258670	MtrunA17_Chr6g0482731	37.037	135	84	1	6	139	26	160	2.83e-22	87.0
Msa1258670	MtrunA17_Chr3g0121191	38.519	135	72	3	6	139	93	217	3.41e-21	85.5
Msa1258670	MtrunA17_Chr7g0254731	35.000	140	81	3	6	139	12	147	5.89e-21	82.8
Msa1258670	MtrunA17_Chr6g0460211	35.714	140	80	3	6	139	12	147	6.71e-21	82.8
Msa1258670	MtrunA17_Chr5g0440931	35.714	140	80	3	6	139	12	147	7.31e-21	82.4
Msa1258670	MtrunA17_Chr4g0044961	35.821	134	85	1	6	139	57	189	8.57e-21	83.6
Msa1258670	MtrunA17_Chr1g0169621	30.864	162	84	2	6	139	4	165	8.96e-21	82.8
Msa1258670	MtrunA17_Chr3g0093271	35.714	140	80	3	6	139	63	198	3.31e-20	82.0
Msa1258670	MtrunA17_Chr1g0188171	34.286	140	83	2	5	139	11	146	6.10e-20	80.1
Msa1258670	MtrunA17_Chr8g0351681	35.417	144	84	3	6	140	49	192	2.84e-19	79.7
Msa1258670	MtrunA17_Chr7g0256171	34.783	138	87	2	6	140	5	142	3.85e-19	78.2
Msa1258670	MtrunA17_Chr7g0256701	33.793	145	82	4	6	140	73	213	4.78e-19	79.3
Msa1258670	MtrunA17_Chr1g0166281	33.824	136	89	1	5	139	11	146	8.66e-19	77.4
Msa1258670	MtrunA17_Chr8g0366401	32.847	137	82	2	6	135	17	150	3.12e-18	76.3
Msa1258670	MtrunA17_Chr2g0329371	37.143	140	83	2	6	140	64	203	5.56e-18	76.6
Msa1258670	MtrunA17_Chr1g0185001	32.593	135	90	1	6	139	12	146	5.76e-18	75.1
Msa1258670	MtrunA17_Chr5g0402331	33.333	135	89	1	6	139	48	182	6.05e-18	75.9
Msa1258670	MtrunA17_Chr7g0245371	32.667	150	88	2	3	139	21	170	3.85e-17	73.6
Msa1258670	MtrunA17_Chr5g0402361	33.333	135	89	1	6	139	220	354	5.23e-17	75.9
Msa1258670	MtrunA17_Chr5g0402361	32.353	136	88	2	6	139	48	181	2.35e-15	71.2
Msa1258670	MtrunA17_Chr4g0064231	33.766	154	83	3	6	140	64	217	2.61e-16	72.4
Msa1258670	MtrunA17_Chr5g0409441	32.031	128	86	1	14	140	30	157	2.86e-15	68.6
Msa1258670	MtrunA17_Chr5g0397101	36.220	127	76	2	15	139	380	503	3.83e-15	70.9
Msa1258670	MtrunA17_Chr4g0062221	29.927	137	93	1	6	139	11	147	8.42e-15	67.4
Msa1258670	MtrunA17_Chr3g0125311	33.333	150	87	4	3	139	10	159	3.62e-14	65.5
Msa1258670	MtrunA17_Chr8g0387671	33.858	127	79	2	15	139	380	503	5.16e-14	67.8
Msa1258670	MtrunA17_Chr5g0444171	32.576	132	84	2	10	139	401	529	7.37e-14	67.4
Msa1258670	MtrunA17_Chr6g0450921	31.111	135	92	1	6	139	29	163	1.02e-13	64.7
Msa1258670	MtrunA17_Chr1g0157131	28.148	135	92	2	7	139	380	511	1.95e-13	66.2
Msa1258670	MtrunA17_Chr5g0396261	33.043	115	66	2	31	138	2	112	2.76e-13	62.0
Msa1258670	MtrunA17_Chr3g0098951	30.882	136	88	2	10	139	392	527	3.23e-13	65.5
Msa1258670	MtrunA17_Chr4g0074811	32.283	127	81	2	15	139	405	528	1.08e-12	63.9
Msa1258670	MtrunA17_Chr1g0160151	30.345	145	84	3	6	139	22	160	1.10e-12	61.6
Msa1258670	MtrunA17_Chr7g0245231	30.370	135	93	1	6	139	30	164	1.12e-12	61.6
Msa1258670	MtrunA17_Chr8g0368681	26.277	137	98	1	6	139	12	148	2.58e-12	60.5
Msa1258670	MtrunA17_Chr5g0441691	28.467	137	93	2	5	139	372	505	4.67e-12	62.0
Msa1258670	MtrunA17_Chr0c02g0489761	29.921	127	84	2	15	139	392	515	7.45e-12	61.6
Msa1258670	MtrunA17_Chr7g0274101	31.852	135	91	1	6	139	12	146	1.02e-11	58.9
Msa1258670	MtrunA17_Chr4g0033371	31.387	137	87	3	7	140	378	510	1.03e-11	61.2
Msa1258670	MtrunA17_Chr1g0173541	31.746	126	86	0	15	140	366	491	1.82e-11	60.5
Msa1258670	MtrunA17_Chr7g0242561	29.078	141	97	1	3	140	355	495	6.59e-11	58.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1258670	AT1G76650.1	47.794	136	71	0	4	139	41	176	5.43e-37	125
Msa1258670	AT1G76650.3	47.794	136	71	0	4	139	41	176	5.43e-37	125
Msa1258670	AT5G42380.1	47.794	136	69	1	6	139	49	184	4.93e-34	117
Msa1258670	AT1G76650.2	46.324	136	63	1	4	139	41	166	4.07e-33	114
Msa1258670	AT1G76640.1	44.604	139	74	1	4	139	20	158	1.80e-32	112
Msa1258670	AT3G50770.1	44.755	143	73	2	3	139	61	203	9.43e-32	112
Msa1258670	AT1G24620.1	45.185	135	73	1	6	139	37	171	8.00e-31	109
Msa1258670	AT3G01830.1	43.885	139	71	4	6	140	11	146	2.13e-28	102
Msa1258670	AT1G73630.1	36.496	137	84	1	3	139	17	150	5.56e-26	96.3
Msa1258670	AT1G18210.2	38.129	139	83	1	1	139	18	153	9.80e-26	95.9
Msa1258670	AT1G18210.1	38.129	139	83	1	1	139	18	153	9.80e-26	95.9
Msa1258670	AT5G37770.1	35.971	139	84	1	6	139	17	155	1.05e-25	95.5
Msa1258670	AT5G37770.2	35.971	139	84	1	6	139	17	155	1.05e-25	95.5
Msa1258670	AT1G66400.1	33.577	137	88	1	6	139	15	151	8.60e-24	90.5
Msa1258670	AT4G03290.1	39.007	141	80	2	6	140	5	145	5.39e-23	88.6
Msa1258670	AT1G05990.1	38.129	139	82	2	6	140	5	143	9.16e-22	85.1
Msa1258670	AT3G59440.1	35.714	140	88	1	3	140	48	187	1.89e-21	85.5
Msa1258670	AT4G12860.1	37.956	137	82	2	6	139	5	141	2.28e-21	84.3
Msa1258670	AT1G66410.1	36.429	140	79	3	6	139	12	147	3.91e-21	83.6
Msa1258670	AT5G37780.1	36.429	140	79	3	6	139	12	147	3.91e-21	83.6
Msa1258670	AT5G21274.1	36.429	140	79	3	6	139	12	147	4.04e-21	83.6
Msa1258670	AT3G56800.1	35.714	140	80	3	6	139	12	147	6.56e-21	83.2
Msa1258670	AT2G41110.1	35.714	140	80	3	6	139	12	147	6.56e-21	83.2
Msa1258670	AT2G27030.1	35.714	140	80	3	6	139	12	147	6.56e-21	83.2
Msa1258670	AT3G43810.1	35.714	140	80	3	6	139	12	147	9.26e-21	82.8
Msa1258670	AT2G43290.1	34.722	144	84	3	6	140	65	207	1.45e-20	84.0
Msa1258670	AT2G27030.3	35.714	140	80	3	6	139	12	147	1.51e-20	82.8
Msa1258670	AT3G22930.1	34.307	137	88	2	5	139	34	170	2.61e-20	82.0
Msa1258670	AT5G17470.1	35.294	136	81	2	7	139	7	138	3.23e-20	81.3
Msa1258670	AT3G43810.2	35.714	140	80	3	6	139	12	147	3.69e-20	82.8
Msa1258670	AT2G15680.1	37.778	135	81	2	6	139	50	182	4.26e-20	82.0
Msa1258670	AT3G07490.1	36.957	138	84	2	6	140	5	142	6.42e-20	80.5
Msa1258670	AT4G14640.1	34.532	139	82	2	6	139	13	147	1.33e-18	77.0
Msa1258670	AT2G36180.1	34.328	134	84	2	10	139	4	137	1.65e-18	76.6
Msa1258670	AT3G10190.1	32.143	140	93	2	3	140	67	206	2.55e-18	77.8
Msa1258670	AT2G41110.2	32.895	152	80	4	6	139	12	159	3.95e-18	76.3
Msa1258670	AT2G41100.7	28.947	152	90	1	6	139	66	217	1.92e-17	76.6
Msa1258670	AT2G41100.6	28.947	152	90	1	6	139	66	217	1.92e-17	76.6
Msa1258670	AT2G41100.3	28.947	152	90	1	6	139	66	217	1.92e-17	76.6
Msa1258670	AT2G41100.4	28.947	152	90	1	6	139	101	252	3.04e-17	76.6
Msa1258670	AT2G41100.4	27.152	151	93	1	6	139	12	162	3.57e-14	68.2
Msa1258670	AT2G41100.1	28.947	152	90	1	6	139	101	252	3.04e-17	76.6
Msa1258670	AT2G41100.1	27.152	151	93	1	6	139	12	162	3.57e-14	68.2
Msa1258670	AT5G37780.2	32.903	155	79	4	6	139	12	162	4.61e-17	73.6
Msa1258670	AT3G50360.1	31.852	135	91	1	6	139	27	161	1.24e-15	69.7
Msa1258670	AT4G23650.1	34.884	129	79	2	14	140	391	516	2.14e-15	72.0
Msa1258670	AT5G37780.3	30.723	166	79	4	6	139	12	173	2.50e-15	69.3
Msa1258670	AT3G51920.1	31.852	135	91	1	6	139	12	146	4.45e-15	68.2
Msa1258670	AT4G20780.1	30.769	156	87	3	5	139	28	183	6.66e-15	68.6
Msa1258670	AT3G03400.1	33.088	136	82	4	3	132	2	134	8.85e-15	67.0
Msa1258670	AT5G37780.4	33.858	127	72	3	20	139	13	134	9.13e-15	67.0
Msa1258670	AT1G66410.2	34.127	126	73	3	20	139	36	157	1.02e-14	67.4
Msa1258670	AT1G18530.1	29.197	137	94	1	6	139	7	143	1.26e-14	67.0
Msa1258670	AT4G37010.2	28.676	136	96	1	6	140	31	166	2.28e-14	66.6
Msa1258670	AT4G37010.1	28.676	136	96	1	6	140	27	162	2.84e-14	66.2
Msa1258670	AT4G04695.1	31.111	135	88	2	7	139	337	468	3.06e-14	68.9
Msa1258670	AT4G04700.1	31.852	135	87	2	7	139	337	468	6.83e-14	67.8
Msa1258670	AT2G41090.1	31.111	135	90	2	6	139	12	144	1.13e-13	65.1
Msa1258670	AT1G66410.4	33.913	115	66	3	31	139	1	111	1.80e-13	62.8
Msa1258670	AT1G66410.3	33.913	115	66	3	31	139	1	111	1.80e-13	62.8
Msa1258670	AT4G14640.2	32.800	125	75	2	20	139	9	129	2.59e-13	63.2
Msa1258670	AT1G62820.1	35.714	126	72	4	14	135	21	141	3.78e-13	62.8
Msa1258670	AT3G43810.4	33.043	115	67	3	31	139	1	111	4.18e-13	62.0
Msa1258670	AT3G43810.3	33.043	115	67	3	31	139	1	111	4.18e-13	62.0
Msa1258670	AT1G12310.1	35.714	126	72	4	14	135	21	141	5.42e-13	62.4
Msa1258670	AT2G41410.1	31.884	138	90	3	6	139	70	207	6.72e-13	63.5
Msa1258670	AT3G51850.1	30.952	126	87	0	15	140	368	493	6.74e-13	65.1
Msa1258670	AT1G32250.1	29.286	140	86	2	6	135	16	152	1.15e-12	62.0
Msa1258670	AT5G44460.1	29.530	149	92	3	5	140	27	175	1.17e-12	62.4
Msa1258670	AT1G76040.1	33.071	127	80	2	15	139	187	310	1.29e-12	63.9
Msa1258670	AT1G76040.4	33.071	127	80	2	15	139	187	310	1.29e-12	63.9
Msa1258670	AT2G31500.1	34.014	147	85	5	3	140	365	508	1.51e-12	63.9
Msa1258670	AT4G04720.2	31.061	132	86	2	10	139	388	516	1.64e-12	63.9
Msa1258670	AT4G04720.1	31.061	132	86	2	10	139	388	516	1.64e-12	63.9
Msa1258670	AT1G76040.2	33.071	127	80	2	15	139	425	548	1.79e-12	63.9
Msa1258670	AT1G50700.1	30.303	132	87	2	10	139	382	510	1.83e-12	63.5
Msa1258670	AT5G19360.1	32.283	127	81	2	15	139	382	505	2.34e-12	63.5
Msa1258670	AT3G01830.2	41.509	106	55	4	6	107	11	113	2.39e-12	60.5
Msa1258670	AT3G25600.1	27.737	137	96	1	6	139	12	148	2.42e-12	61.2
Msa1258670	AT1G74740.1	29.545	132	91	2	10	140	368	498	3.49e-12	62.8
Msa1258670	AT3G20410.2	31.061	132	86	2	10	139	400	528	4.16e-12	62.8
Msa1258670	AT3G20410.1	31.061	132	86	2	10	139	400	528	4.16e-12	62.8
Msa1258670	AT3G03000.1	30.303	132	90	1	6	135	20	151	4.34e-12	60.5
Msa1258670	AT4G21940.1	32.283	127	81	2	15	139	415	538	4.42e-12	62.8
Msa1258670	AT4G21940.2	32.283	127	81	2	15	139	415	538	4.48e-12	62.8
Msa1258670	AT5G12180.1	31.496	127	82	2	15	139	387	510	1.54e-11	61.2
Msa1258670	AT5G12480.2	31.496	127	86	1	15	140	279	405	3.85e-11	59.7
Msa1258670	AT2G41100.2	29.630	108	58	1	50	139	20	127	7.01e-11	57.8
Msa1258670	AT1G61950.1	30.075	133	88	2	10	140	408	537	7.33e-11	58.9

Find 35 sgRNAs with CRISPR-Local

Find 39 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGAGGATTTAATAACGCTTA+TGG	0.268235	8_2:-59905642	Msa1258670:CDS
AACATCCTCTTCAAACTCTT+TGG	0.271094	8_2:+59905536	None:intergenic
CATCACTTTACATTCTTCAA+TGG	0.327118	8_2:+59905490	None:intergenic
GATGATTGGAAGGTTTGATT+TGG	0.354750	8_2:-59905471	Msa1258670:CDS
TTAATAACGCTTATGGAATC+CGG	0.394530	8_2:-59905635	Msa1258670:CDS
AGATTTCACCGAACGAGTTA+AGG	0.395728	8_2:-59905760	Msa1258670:CDS
AAGAAAAGTTGAAGGATTTG+AGG	0.418092	8_2:-59905604	Msa1258670:CDS
GGAAATTGCTATTGAGGCAT+TGG	0.422915	8_2:-59905693	Msa1258670:CDS
TGGTGATGGGCTATTGAGTT+TGG	0.423743	8_2:-59905663	Msa1258670:CDS
AGAAAAGTTGAAGGATTTGA+GGG	0.426919	8_2:-59905603	Msa1258670:CDS
AGGAGTCGAATGACGAAGAT+TGG	0.473202	8_2:-59905740	Msa1258670:CDS
ATTCGACTCCTTAACTCGTT+CGG	0.474759	8_2:+59905752	None:intergenic
GAAGAATGTAAAGTGATGAT+TGG	0.486801	8_2:-59905485	Msa1258670:CDS
TAATAACGCTTATGGAATCC+GGG	0.494077	8_2:-59905634	Msa1258670:CDS
AGAAGTGGAAATTGCTATTG+AGG	0.499700	8_2:-59905699	Msa1258670:CDS
AATGTAAAGTGATGATTGGA+AGG	0.500397	8_2:-59905481	Msa1258670:CDS
AGAGTTTCAATTGAAAGAAG+TGG	0.502556	8_2:-59905714	Msa1258670:CDS
TGAAGAATTCAGAATCATGA+TGG	0.506151	8_2:-59905426	Msa1258670:CDS
AGGTTTGATTTGGATGGTGA+TGG	0.507936	8_2:-59905461	Msa1258670:CDS
ATTGGAAGGTTTGATTTGGA+TGG	0.510943	8_2:-59905467	Msa1258670:CDS
TGATGGGCTATTGAGTTTGG+AGG	0.520299	8_2:-59905660	Msa1258670:CDS
TTTGAAATGTATGATAATGA+AGG	0.534288	8_2:-59905575	Msa1258670:CDS
ATGTATGATAATGAAGGATG+TGG	0.537649	8_2:-59905569	Msa1258670:CDS
GCATTGGATTCTGATGGTGA+TGG	0.540839	8_2:-59905677	Msa1258670:CDS
TACTTTGATGAAGACGGTGA+TGG	0.542295	8_2:-59905785	Msa1258670:CDS
GGGAGAAGAAGAAAAGTTGA+AGG	0.545380	8_2:-59905612	Msa1258670:CDS
CTAAGTTACTTTGATGAAGA+CGG	0.574980	8_2:-59905791	Msa1258670:CDS
AGTCGAATGACGAAGATTGG+CGG	0.582253	8_2:-59905737	Msa1258670:CDS
CATTGGATTCTGATGGTGAT+GGG	0.600002	8_2:-59905676	Msa1258670:CDS
TAACACCAAAGAGTTTGAAG+AGG	0.608054	8_2:-59905541	Msa1258670:CDS
AATTAACATGAGGAGTAACA+TGG	0.611175	8_2:-59905828	None:intergenic
ATTGAGGCATTGGATTCTGA+TGG	0.616237	8_2:-59905683	Msa1258670:CDS
TGTATGATAATGAAGGATGT+GGG	0.638642	8_2:-59905568	Msa1258670:CDS
AATAACGCTTATGGAATCCG+GGG	0.638897	8_2:-59905633	Msa1258670:CDS
ATAACGCTTATGGAATCCGG+GGG	0.690847	8_2:-59905632	Msa1258670:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTGAAATGTATGATAATGA+AGG	-	chr8_2:59905667-59905686	Msa1258670:CDS	20.0%
!	CTAAGTTACTTTGATGAAGA+CGG	-	chr8_2:59905451-59905470	Msa1258670:CDS	30.0%
	AGAGTTTCAATTGAAAGAAG+TGG	-	chr8_2:59905528-59905547	Msa1258670:CDS	30.0%
	TTAATAACGCTTATGGAATC+CGG	-	chr8_2:59905607-59905626	Msa1258670:CDS	30.0%
	AAGAAAAGTTGAAGGATTTG+AGG	-	chr8_2:59905638-59905657	Msa1258670:CDS	30.0%
	AGAAAAGTTGAAGGATTTGA+GGG	-	chr8_2:59905639-59905658	Msa1258670:CDS	30.0%
!	ATGTATGATAATGAAGGATG+TGG	-	chr8_2:59905673-59905692	Msa1258670:CDS	30.0%
!	TGTATGATAATGAAGGATGT+GGG	-	chr8_2:59905674-59905693	Msa1258670:CDS	30.0%
	AAGAGGATGTTGAAAAAGTT+GGG	-	chr8_2:59905718-59905737	Msa1258670:CDS	30.0%
	CATCACTTTACATTCTTCAA+TGG	+	chr8_2:59905755-59905774	None:intergenic	30.0%
	GAAGAATGTAAAGTGATGAT+TGG	-	chr8_2:59905757-59905776	Msa1258670:CDS	30.0%
	AATGTAAAGTGATGATTGGA+AGG	-	chr8_2:59905761-59905780	Msa1258670:CDS	30.0%
	TGAAGAATTCAGAATCATGA+TGG	-	chr8_2:59905816-59905835	Msa1258670:CDS	30.0%
!	AGAAGTGGAAATTGCTATTG+AGG	-	chr8_2:59905543-59905562	Msa1258670:CDS	35.0%
	GGAGGATTTAATAACGCTTA+TGG	-	chr8_2:59905600-59905619	Msa1258670:CDS	35.0%
	TAATAACGCTTATGGAATCC+GGG	-	chr8_2:59905608-59905627	Msa1258670:CDS	35.0%
	TAACACCAAAGAGTTTGAAG+AGG	-	chr8_2:59905701-59905720	Msa1258670:CDS	35.0%
	AACATCCTCTTCAAACTCTT+TGG	+	chr8_2:59905709-59905728	None:intergenic	35.0%
	GAAGAGGATGTTGAAAAAGT+TGG	-	chr8_2:59905717-59905736	Msa1258670:CDS	35.0%
	AGAGGATGTTGAAAAAGTTG+GGG	-	chr8_2:59905719-59905738	Msa1258670:CDS	35.0%
	GATGATTGGAAGGTTTGATT+TGG	-	chr8_2:59905771-59905790	Msa1258670:CDS	35.0%
!!	ATTGGAAGGTTTGATTTGGA+TGG	-	chr8_2:59905775-59905794	Msa1258670:CDS	35.0%
	TACTTTGATGAAGACGGTGA+TGG	-	chr8_2:59905457-59905476	Msa1258670:CDS	40.0%
	AGATTTCACCGAACGAGTTA+AGG	-	chr8_2:59905482-59905501	Msa1258670:CDS	40.0%
	ATTCGACTCCTTAACTCGTT+CGG	+	chr8_2:59905493-59905512	None:intergenic	40.0%
!	GGAAATTGCTATTGAGGCAT+TGG	-	chr8_2:59905549-59905568	Msa1258670:CDS	40.0%
!!	ATTGAGGCATTGGATTCTGA+TGG	-	chr8_2:59905559-59905578	Msa1258670:CDS	40.0%
!	CATTGGATTCTGATGGTGAT+GGG	-	chr8_2:59905566-59905585	Msa1258670:CDS	40.0%
	AATAACGCTTATGGAATCCG+GGG	-	chr8_2:59905609-59905628	Msa1258670:CDS	40.0%
	GGGAGAAGAAGAAAAGTTGA+AGG	-	chr8_2:59905630-59905649	Msa1258670:CDS	40.0%
	GAGGATGTTGAAAAAGTTGG+GGG	-	chr8_2:59905720-59905739	Msa1258670:CDS	40.0%
!!	AGGTTTGATTTGGATGGTGA+TGG	-	chr8_2:59905781-59905800	Msa1258670:CDS	40.0%
	AGGAGTCGAATGACGAAGAT+TGG	-	chr8_2:59905502-59905521	Msa1258670:CDS	45.0%
	AGTCGAATGACGAAGATTGG+CGG	-	chr8_2:59905505-59905524	Msa1258670:CDS	45.0%
!	GCATTGGATTCTGATGGTGA+TGG	-	chr8_2:59905565-59905584	Msa1258670:CDS	45.0%
!!	TGGTGATGGGCTATTGAGTT+TGG	-	chr8_2:59905579-59905598	Msa1258670:CDS	45.0%
!!	TGATGGGCTATTGAGTTTGG+AGG	-	chr8_2:59905582-59905601	Msa1258670:CDS	45.0%
	ATAACGCTTATGGAATCCGG+GGG	-	chr8_2:59905610-59905629	Msa1258670:CDS	45.0%
!	CAACTTTTCTTCTTCTCCCC+CGG	+	chr8_2:59905629-59905648	None:intergenic	45.0%

Chromosome	Type	Strat	End	Strand	Name
chr8_2	gene	59905421	59905843	59905421	ID=Msa1258670;Name=Msa1258670
chr8_2	mRNA	59905421	59905843	59905421	ID=Msa1258670-mRNA-1;Parent=Msa1258670;Name=Msa1258670-mRNA-1;_AED=0.34;_eAED=0.34;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|140
chr8_2	exon	59905421	59905843	59905421	ID=Msa1258670-mRNA-1:exon:18796;Parent=Msa1258670-mRNA-1
chr8_2	CDS	59905421	59905843	59905421	ID=Msa1258670-mRNA-1:cds;Parent=Msa1258670-mRNA-1

Gene Sequence

>Msa1258670

ATGAGGAGTAACATGGAATTTGAGCGTGTACTAAGTTACTTTGATGAAGACGGTGATGGAAAGATTTCACCGAACGAGTTAAGGAGTCGAATGACGAAGATTGGCGGAGAGTTTCAATTGAAAGAAGTGGAAATTGCTATTGAGGCATTGGATTCTGATGGTGATGGGCTATTGAGTTTGGAGGATTTAATAACGCTTATGGAATCCGGGGGAGAAGAAGAAAAGTTGAAGGATTTGAGGGAAGCTTTTGAAATGTATGATAATGAAGGATGTGGGTTCATAACACCAAAGAGTTTGAAGAGGATGTTGAAAAAGTTGGGGGATTCTAAGTCCATTGAAGAATGTAAAGTGATGATTGGAAGGTTTGATTTGGATGGTGATGGTGTGCTTAGTTTTGAAGAATTCAGAATCATGATGGAGTGA

Protein sequence

>Msa1258670

MRSNMEFERVLSYFDEDGDGKISPNELRSRMTKIGGEFQLKEVEIAIEALDSDGDGLLSLEDLITLMESGGEEEKLKDLREAFEMYDNEGCGFITPKSLKRMLKKLGDSKSIEECKVMIGRFDLDGDGVLSFEEFRIMME*