AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0305620

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0305620 MtrunA17_Chr2g0333851 95.327 321 6 5 1 317 1 316 0.0 613
Msa0305620 MtrunA17_Chr4g0001361 59.816 326 106 8 5 317 4 317 2.61e-107 315
Msa0305620 MtrunA17_Chr1g0150281 46.643 283 127 7 41 314 9 276 4.72e-71 221
Msa0305620 MtrunA17_Chr3g0136961 46.545 275 114 7 56 314 29 286 2.53e-70 220
Msa0305620 MtrunA17_Chr1g0150271 35.897 273 129 8 51 316 6 239 9.26e-40 140
Msa0305620 MtrunA17_Chr7g0270831 27.848 316 149 9 57 310 100 398 1.41e-29 117
Msa0305620 MtrunA17_Chr4g0075201 30.357 224 113 5 49 236 116 332 1.05e-27 112
Msa0305620 MtrunA17_Chr2g0288871 32.911 237 95 8 49 231 139 365 3.65e-26 108
Msa0305620 MtrunA17_Chr2g0302391 31.579 266 123 8 55 275 70 321 3.37e-24 102
Msa0305620 MtrunA17_Chr3g0099641 30.870 230 110 6 47 231 86 311 3.45e-24 102
Msa0305620 MtrunA17_Chr4g0051181 53.086 81 33 2 151 231 228 303 1.17e-23 100
Msa0305620 MtrunA17_Chr7g0216891 47.619 84 42 1 146 229 196 277 1.20e-22 98.2
Msa0305620 MtrunA17_Chr4g0060271 51.250 80 37 1 152 231 238 315 1.49e-22 98.2
Msa0305620 MtrunA17_Chr7g0270191 50.617 81 37 2 151 231 127 204 3.09e-22 94.4
Msa0305620 MtrunA17_Chr1g0194481 54.545 77 32 2 156 232 145 218 3.42e-22 94.7
Msa0305620 MtrunA17_Chr2g0294231 53.086 81 35 2 156 236 190 267 9.24e-22 94.4
Msa0305620 MtrunA17_Chr4g0051881 51.948 77 34 2 156 232 165 238 1.81e-21 93.2
Msa0305620 MtrunA17_Chr4g0025051 50.000 78 37 1 155 232 321 396 3.29e-21 94.0
Msa0305620 MtrunA17_Chr2g0322391 31.174 247 88 9 57 231 173 409 1.60e-20 92.0
Msa0305620 MtrunA17_Chr1g0188941 41.667 96 40 2 137 232 32 111 1.83e-20 85.1
Msa0305620 MtrunA17_Chr5g0445311 57.812 64 26 1 169 232 362 424 4.61e-20 90.5
Msa0305620 MtrunA17_Chr5g0445311 46.269 67 31 3 172 238 201 262 1.22e-11 65.5
Msa0305620 MtrunA17_Chr4g0025061 50.000 78 37 1 155 232 346 421 5.02e-20 90.5
Msa0305620 MtrunA17_Chr4g0004911 40.909 110 49 2 122 231 302 395 5.87e-20 90.1
Msa0305620 MtrunA17_Chr4g0004911 49.231 65 32 1 171 235 184 247 3.62e-16 79.0
Msa0305620 MtrunA17_Chr7g0263531 52.857 70 32 1 162 231 352 420 7.20e-20 89.7
Msa0305620 MtrunA17_Chr7g0263531 53.333 60 26 2 172 231 190 247 9.65e-15 74.3
Msa0305620 MtrunA17_Chr5g0418191 54.688 64 28 1 169 232 318 380 8.57e-20 89.4
Msa0305620 MtrunA17_Chr5g0418191 45.455 77 32 4 172 246 152 220 6.04e-13 68.9
Msa0305620 MtrunA17_Chr3g0101131 57.812 64 26 1 169 232 413 475 8.78e-20 89.7
Msa0305620 MtrunA17_Chr3g0101131 48.333 60 29 2 172 231 244 301 1.49e-11 65.1
Msa0305620 MtrunA17_Chr4g0042831 42.056 107 46 3 137 243 259 349 9.39e-20 89.0
Msa0305620 MtrunA17_Chr4g0042831 48.529 68 34 1 172 239 146 212 3.43e-13 69.7
Msa0305620 MtrunA17_Chr5g0443131 50.649 77 35 2 156 232 144 217 1.21e-19 87.4
Msa0305620 MtrunA17_Chr1g0150801 50.667 75 34 2 156 230 89 160 3.40e-19 83.2
Msa0305620 MtrunA17_Chr3g0079691 41.667 96 40 2 137 232 476 555 5.45e-19 87.8
Msa0305620 MtrunA17_Chr3g0079691 34.783 115 47 4 171 285 280 366 1.98e-12 67.8
Msa0305620 MtrunA17_Chr7g0218081 56.667 60 25 1 169 228 408 466 6.22e-19 87.0
Msa0305620 MtrunA17_Chr7g0218081 43.373 83 43 3 172 252 229 309 1.50e-11 65.1
Msa0305620 MtrunA17_Chr5g0432161 40.741 108 48 3 125 232 390 481 6.41e-19 87.4
Msa0305620 MtrunA17_Chr5g0432161 50.794 63 29 2 172 234 245 305 4.01e-14 72.8
Msa0305620 MtrunA17_Chr5g0401821 40.385 104 55 2 125 228 160 256 7.76e-19 84.7
Msa0305620 MtrunA17_Chr7g0243891 38.318 107 50 2 126 232 344 434 8.76e-19 86.7
Msa0305620 MtrunA17_Chr7g0243891 45.714 70 36 2 171 240 216 283 2.60e-14 73.6
Msa0305620 MtrunA17_Chr5g0418781 50.685 73 33 2 156 228 111 180 1.05e-18 84.3
Msa0305620 MtrunA17_Chr8g0383351 49.315 73 35 2 156 228 146 216 1.07e-18 83.6
Msa0305620 MtrunA17_Chr7g0224801 38.095 126 64 3 115 229 28 150 1.50e-18 81.6
Msa0305620 MtrunA17_Chr7g0249311 44.545 110 54 4 126 232 347 452 1.67e-18 86.3
Msa0305620 MtrunA17_Chr7g0249311 49.231 65 29 3 171 233 179 241 1.05e-11 65.5
Msa0305620 MtrunA17_Chr7g0248721 51.282 78 34 3 152 229 124 197 2.01e-18 82.4
Msa0305620 MtrunA17_Chr7g0249301 43.750 96 51 3 139 232 258 352 4.03e-18 84.0
Msa0305620 MtrunA17_Chr7g0249301 46.774 62 31 2 170 231 61 120 4.19e-11 63.2
Msa0305620 MtrunA17_Chr1g0147881 44.048 84 43 1 149 228 236 319 4.39e-18 83.6
Msa0305620 MtrunA17_Chr4g0068541 49.351 77 36 2 155 231 194 267 4.43e-18 84.3
Msa0305620 MtrunA17_Chr3g0106491 39.091 110 51 3 123 232 332 425 5.19e-18 84.3
Msa0305620 MtrunA17_Chr3g0106491 50.000 60 28 2 172 231 199 256 4.66e-14 72.4
Msa0305620 MtrunA17_Chr8g0386241 44.872 78 42 1 169 246 101 177 6.45e-18 80.1
Msa0305620 MtrunA17_Chr4g0053281 56.140 57 25 0 172 228 267 323 9.99e-18 82.4
Msa0305620 MtrunA17_Chr3g0129321 30.570 193 110 6 88 273 30 205 1.02e-17 80.1
Msa0305620 MtrunA17_Chr5g0447701 50.000 78 36 2 155 232 238 312 1.10e-17 82.8
Msa0305620 MtrunA17_Chr3g0129361 47.945 73 35 2 156 228 131 200 2.07e-17 79.7
Msa0305620 MtrunA17_Chr8g0383251 47.368 76 37 2 156 231 95 167 6.39e-17 77.4
Msa0305620 MtrunA17_Chr8g0335811 39.130 115 60 4 115 228 105 210 8.15e-17 78.2
Msa0305620 MtrunA17_Chr2g0293481 43.038 79 45 0 168 246 264 342 8.82e-17 80.9
Msa0305620 MtrunA17_Chr1g0184691 51.667 60 29 0 169 228 291 350 1.18e-16 79.7
Msa0305620 MtrunA17_Chr7g0270051 35.238 105 65 1 135 239 20 121 1.36e-16 78.2
Msa0305620 MtrunA17_Chr7g0238921 45.333 75 30 2 172 236 125 198 4.32e-16 77.4
Msa0305620 MtrunA17_Chr4g0071521 45.570 79 42 1 151 228 224 302 5.09e-16 77.4
Msa0305620 MtrunA17_Chr8g0387501 46.774 62 33 0 172 233 130 191 1.12e-15 75.9
Msa0305620 MtrunA17_Chr8g0348311 37.895 95 53 2 172 266 156 244 1.22e-15 76.6
Msa0305620 MtrunA17_Chr7g0228941 43.939 66 37 0 163 228 44 109 1.22e-15 72.4
Msa0305620 MtrunA17_Chr8g0379461 35.870 92 59 0 140 231 45 136 4.95e-15 73.6
Msa0305620 MtrunA17_Chr4g0024391 38.462 78 46 1 155 232 215 290 4.96e-15 75.5
Msa0305620 MtrunA17_Chr3g0139021 31.818 110 58 2 124 228 274 371 5.88e-15 75.1
Msa0305620 MtrunA17_Chr4g0074001 43.836 73 40 1 159 231 347 418 6.02e-15 75.1
Msa0305620 MtrunA17_Chr7g0248691 43.077 65 37 0 169 233 152 216 9.04e-15 73.9
Msa0305620 MtrunA17_Chr5g0432071 39.394 99 52 4 172 269 105 196 2.96e-14 70.5
Msa0305620 MtrunA17_Chr2g0284321 45.000 60 33 0 172 231 36 95 3.99e-14 70.5
Msa0305620 MtrunA17_Chr1g0189391 32.800 125 70 5 111 228 37 154 6.03e-14 71.6
Msa0305620 MtrunA17_Chr2g0289181 45.161 62 34 0 171 232 14 75 6.74e-14 70.1
Msa0305620 MtrunA17_Chr2g0319961 36.364 110 66 2 125 231 32 140 1.12e-13 69.7
Msa0305620 MtrunA17_Chr6g0485431 38.202 89 50 4 149 235 133 218 1.36e-13 70.5
Msa0305620 MtrunA17_Chr1g0202461 43.548 62 35 0 172 233 262 323 7.81e-13 68.6
Msa0305620 MtrunA17_Chr5g0447711 38.667 75 45 1 158 232 217 290 2.15e-12 66.6
Msa0305620 MtrunA17_Chr8g0387981 47.059 68 32 2 163 226 122 189 7.35e-12 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0305620 AT1G80840.1 53.776 331 106 12 1 317 1 298 5.81e-99 294
Msa0305620 AT4G31800.1 44.940 336 136 10 1 316 1 307 6.68e-79 243
Msa0305620 AT4G31800.2 46.947 262 100 7 74 316 1 242 1.05e-67 212
Msa0305620 AT2G25000.2 45.660 265 94 8 57 314 2 223 2.19e-63 200
Msa0305620 AT2G25000.4 45.660 265 94 8 57 314 2 223 2.19e-63 200
Msa0305620 AT2G25000.1 44.765 277 99 8 49 316 37 268 2.58e-63 202
Msa0305620 AT2G25000.3 43.986 291 106 9 33 314 3 245 8.37e-63 199
Msa0305620 AT4G31800.3 46.053 228 88 8 1 208 1 213 1.25e-49 165
Msa0305620 AT5G41570.1 48.936 94 44 3 136 229 66 155 2.78e-23 95.1
Msa0305620 AT4G01720.1 25.143 350 152 9 60 310 105 443 4.92e-23 99.4
Msa0305620 AT4G18170.1 47.525 101 49 3 133 232 135 232 6.71e-23 97.1
Msa0305620 AT4G04450.2 39.252 107 56 1 125 231 161 258 8.69e-23 98.2
Msa0305620 AT1G64000.1 52.632 76 33 2 154 229 99 171 8.85e-23 94.0
Msa0305620 AT1G68150.1 51.852 81 34 2 151 231 220 295 1.26e-22 97.4
Msa0305620 AT4G22070.4 50.000 84 37 2 148 231 279 357 1.72e-22 98.2
Msa0305620 AT4G22070.3 50.000 84 37 2 148 231 279 357 1.72e-22 98.2
Msa0305620 AT4G22070.2 50.000 84 37 2 148 231 279 357 1.72e-22 98.2
Msa0305620 AT4G22070.1 50.000 84 37 2 148 231 279 357 1.72e-22 98.2
Msa0305620 AT4G04450.1 39.252 107 56 1 125 231 255 352 2.63e-22 97.4
Msa0305620 AT1G29860.1 52.439 82 36 2 156 237 123 201 2.97e-22 94.7
Msa0305620 AT1G62300.1 50.000 84 37 2 148 231 294 372 4.18e-22 97.1
Msa0305620 AT5G15130.2 38.793 116 55 3 121 231 141 245 1.11e-21 95.5
Msa0305620 AT5G15130.1 38.793 116 55 3 121 231 183 287 1.25e-21 95.5
Msa0305620 AT1G18860.1 58.065 62 26 0 170 231 238 299 1.78e-21 95.1
Msa0305620 AT5G07100.2 33.987 153 80 4 85 232 65 201 3.29e-21 90.5
Msa0305620 AT5G07100.2 45.455 77 41 1 159 235 12 87 3.65e-16 76.6
Msa0305620 AT5G07100.4 33.987 153 80 4 85 232 65 201 3.29e-21 90.5
Msa0305620 AT5G07100.4 45.455 77 41 1 159 235 12 87 3.65e-16 76.6
Msa0305620 AT5G07100.5 33.987 153 80 4 85 232 65 201 3.29e-21 90.5
Msa0305620 AT5G07100.5 45.455 77 41 1 159 235 12 87 3.65e-16 76.6
Msa0305620 AT5G07100.3 33.987 153 80 4 85 232 65 201 3.29e-21 90.5
Msa0305620 AT5G07100.3 45.455 77 41 1 159 235 12 87 3.65e-16 76.6
Msa0305620 AT2G47260.1 50.000 86 40 2 151 236 156 238 5.40e-21 92.0
Msa0305620 AT5G49520.1 51.948 77 34 2 155 231 207 280 6.14e-21 92.8
Msa0305620 AT5G07100.1 33.987 153 80 4 85 232 158 294 7.67e-21 91.3
Msa0305620 AT5G07100.1 45.455 77 41 1 159 235 105 180 1.22e-15 76.6
Msa0305620 AT3G62340.1 53.086 81 35 2 151 231 100 177 9.54e-21 90.5
Msa0305620 AT2G21900.1 44.554 101 48 3 133 232 76 169 1.31e-20 88.2
Msa0305620 AT4G26640.5 32.512 203 119 6 113 310 144 333 1.48e-20 91.7
Msa0305620 AT4G26640.5 43.662 71 38 2 161 231 25 93 3.33e-12 67.0
Msa0305620 AT4G26640.6 32.512 203 119 6 113 310 144 333 1.48e-20 91.7
Msa0305620 AT4G26640.6 43.662 71 38 2 161 231 25 93 3.33e-12 67.0
Msa0305620 AT4G26640.4 32.512 203 119 6 113 310 144 333 1.48e-20 91.7
Msa0305620 AT4G26640.4 43.662 71 38 2 161 231 25 93 3.33e-12 67.0
Msa0305620 AT4G26640.3 32.512 203 119 6 113 310 144 333 1.48e-20 91.7
Msa0305620 AT4G26640.3 43.662 71 38 2 161 231 25 93 3.33e-12 67.0
Msa0305620 AT5G26170.1 53.425 73 31 2 156 228 100 169 2.11e-20 87.0
Msa0305620 AT1G13960.1 34.694 147 68 3 101 247 366 484 2.41e-20 91.7
Msa0305620 AT1G13960.1 50.746 67 31 2 172 238 230 294 2.48e-13 70.9
Msa0305620 AT4G26640.1 32.512 203 119 6 113 310 248 437 3.53e-20 91.3
Msa0305620 AT4G26640.1 43.662 71 38 2 161 231 129 197 3.40e-12 67.4
Msa0305620 AT1G13960.2 34.694 147 68 3 101 247 213 331 4.03e-20 90.1
Msa0305620 AT1G13960.2 50.746 67 31 2 172 238 77 141 2.70e-13 70.1
Msa0305620 AT1G69310.4 50.000 78 36 2 151 228 129 203 5.26e-20 88.6
Msa0305620 AT1G69310.2 50.000 78 36 2 151 228 129 203 5.26e-20 88.6
Msa0305620 AT1G69310.1 50.000 78 36 2 151 228 129 203 5.26e-20 88.6
Msa0305620 AT4G26640.7 36.429 140 81 3 113 247 280 416 5.88e-20 90.5
Msa0305620 AT4G26640.7 43.662 71 38 2 161 231 161 229 3.86e-12 67.4
Msa0305620 AT4G26640.2 36.429 140 81 3 113 247 320 456 6.44e-20 90.5
Msa0305620 AT4G26640.2 43.662 71 38 2 161 231 201 269 3.93e-12 67.4
Msa0305620 AT5G46350.1 40.476 126 54 6 156 280 170 275 8.19e-20 88.6
Msa0305620 AT5G56270.1 57.812 64 26 1 169 232 485 547 9.00e-20 90.5
Msa0305620 AT5G56270.1 44.737 76 36 3 170 245 272 341 1.61e-12 68.6
Msa0305620 AT2G03340.1 56.250 64 27 1 169 232 413 475 9.67e-20 90.1
Msa0305620 AT2G03340.1 47.222 72 36 2 172 243 251 320 4.49e-14 73.2
Msa0305620 AT5G13080.1 50.000 80 37 2 156 235 54 130 1.83e-19 83.6
Msa0305620 AT1G55600.1 50.000 80 37 2 152 231 290 366 2.33e-19 88.6
Msa0305620 AT3G01080.2 56.667 60 25 1 169 228 342 400 1.16e-18 86.7
Msa0305620 AT3G01080.1 56.667 60 25 1 169 228 304 362 1.27e-18 86.3
Msa0305620 AT1G69810.1 29.365 252 113 8 43 231 15 264 1.27e-18 85.9
Msa0305620 AT2G30250.1 48.276 87 42 2 146 232 305 388 3.55e-18 84.7
Msa0305620 AT2G30250.1 35.577 104 52 4 172 262 167 268 1.56e-12 68.2
Msa0305620 AT5G64810.1 37.931 116 67 3 113 228 56 166 4.24e-18 81.3
Msa0305620 AT3G01970.1 51.351 74 33 2 155 228 51 121 6.54e-18 79.7
Msa0305620 AT5G52830.1 42.045 88 51 0 172 259 166 253 8.06e-18 83.2
Msa0305620 AT1G69810.2 48.750 80 38 2 153 231 129 206 8.49e-18 83.2
Msa0305620 AT2G23320.1 39.683 126 62 4 103 228 186 297 1.16e-17 82.4
Msa0305620 AT4G39410.1 45.783 83 42 2 146 228 200 279 1.48e-17 82.0
Msa0305620 AT2G46130.1 48.101 79 38 2 151 229 12 87 1.53e-17 77.4
Msa0305620 AT2G04880.1 50.704 71 34 1 169 239 305 374 1.82e-17 83.2
Msa0305620 AT2G04880.1 40.244 82 46 3 169 249 109 188 1.39e-12 68.6
Msa0305620 AT2G04880.2 50.704 71 34 1 169 239 281 350 2.09e-17 82.8
Msa0305620 AT2G04880.2 40.244 82 46 3 169 249 109 188 1.58e-12 68.2
Msa0305620 AT2G38470.1 53.125 64 29 1 169 232 360 422 2.95e-17 82.8
Msa0305620 AT2G38470.1 39.806 103 54 3 171 273 184 278 1.91e-14 74.3
Msa0305620 AT4G26440.1 54.688 64 28 1 169 232 370 432 4.11e-17 82.4
Msa0305620 AT4G26440.1 44.928 69 32 3 172 240 179 241 1.77e-11 65.5
Msa0305620 AT2G30590.1 50.746 67 32 1 169 234 311 377 4.42e-17 81.6
Msa0305620 AT2G44745.1 38.655 119 68 4 120 236 95 210 4.63e-17 79.0
Msa0305620 AT4G31550.2 51.667 60 29 0 169 228 243 302 5.04e-17 80.9
Msa0305620 AT4G31550.1 51.667 60 29 0 169 228 244 303 5.14e-17 80.9
Msa0305620 AT1G29280.1 50.000 60 30 0 172 231 75 134 6.84e-17 79.3
Msa0305620 AT5G43290.1 42.857 84 46 2 145 228 89 170 7.16e-17 79.7
Msa0305620 AT2G34830.1 35.043 117 70 1 123 233 161 277 8.36e-17 80.9
Msa0305620 AT2G34830.2 35.043 117 70 1 123 233 170 286 1.01e-16 80.9
Msa0305620 AT3G04670.1 51.667 60 29 0 169 228 260 319 2.97e-16 78.6
Msa0305620 AT4G24240.1 44.304 79 43 1 151 228 260 338 4.41e-16 78.2
Msa0305620 AT2G37260.3 37.500 120 64 3 113 228 213 325 4.94e-16 78.2
Msa0305620 AT2G37260.3 48.000 75 37 2 158 232 72 144 3.97e-14 72.4
Msa0305620 AT1G69310.3 48.718 78 33 3 151 228 129 199 5.06e-16 77.4
Msa0305620 AT2G37260.2 37.500 120 64 3 113 228 211 323 5.36e-16 78.2
Msa0305620 AT2G37260.2 48.000 75 37 2 158 232 70 142 4.18e-14 72.4
Msa0305620 AT2G37260.1 37.037 135 69 4 113 238 293 420 7.40e-16 78.2
Msa0305620 AT2G37260.1 48.000 75 37 2 158 232 152 224 4.41e-14 72.8
Msa0305620 AT5G28650.1 51.667 60 29 0 169 228 260 319 8.29e-16 77.4
Msa0305620 AT1G30650.1 45.714 70 38 0 164 233 210 279 8.70e-16 77.8
Msa0305620 AT2G24570.1 50.000 60 30 0 169 228 241 300 1.40e-15 76.6
Msa0305620 AT5G45050.1 37.500 120 66 2 172 282 1181 1300 1.56e-15 77.8
Msa0305620 AT5G45050.2 37.500 120 66 2 172 282 1153 1272 1.64e-15 77.8
Msa0305620 AT5G45050.4 37.500 120 66 2 172 282 1042 1161 1.83e-15 77.8
Msa0305620 AT3G58710.2 40.323 124 64 3 129 245 24 144 2.17e-15 75.5
Msa0305620 AT4G01250.1 38.824 85 52 0 172 256 129 213 2.37e-15 75.5
Msa0305620 AT3G58710.1 43.243 74 42 0 172 245 72 145 3.12e-15 74.7
Msa0305620 AT4G23550.1 32.192 146 87 4 169 314 138 271 3.81e-15 75.1
Msa0305620 AT4G30935.1 42.466 73 41 1 159 231 319 390 4.79e-15 75.9
Msa0305620 AT2G46130.2 46.835 79 35 3 151 229 12 83 1.20e-13 66.6
Msa0305620 AT2G46400.1 41.096 73 39 2 156 226 91 161 9.54e-12 65.1
Msa0305620 AT5G45260.1 37.975 79 47 1 172 248 1209 1287 1.47e-11 65.9
Msa0305620 AT3G01080.3 40.000 75 43 2 172 246 206 278 7.53e-11 62.8

Find 67 sgRNAs with CRISPR-Local

Find 187 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATCTGTGGTACTTCCATTT+TGG 0.140306 2_3:-63523126 None:intergenic
GTTGCTTTGTTTAGAAATTT+TGG 0.157085 2_3:-63523511 None:intergenic
AATGGAAGTACCACAGATTT+TGG 0.248540 2_3:+63523130 Msa0305620:CDS
TCATACTTCTTGAAGCTTAC+AGG 0.255823 2_3:-63522142 None:intergenic
GCACTTGCTGCTGCCATATC+AGG 0.283466 2_3:+63523200 Msa0305620:CDS
AATCTAATGGGAGGAGTAAC+TGG 0.300205 2_3:+63522538 Msa0305620:CDS
GGATATCAGTGGAGAAAATA+TGG 0.332364 2_3:+63522807 Msa0305620:CDS
TGAATAATTTATTTGACATA+TGG 0.338190 2_3:+63522045 Msa0305620:exon
GATGATAAATAATCCCAAAA+TGG 0.343074 2_3:+63523112 Msa0305620:CDS
TTTATCATCATATTCTTAGA+AGG 0.347068 2_3:-63523098 None:intergenic
AATAGTAGAAACTTGCATTA+AGG 0.347618 2_3:+63522618 Msa0305620:CDS
CAAGTACAGTTACACTTGAT+TGG 0.350481 2_3:+63523051 Msa0305620:CDS
AAGCAGCAGTAACAATCTAA+TGG 0.359770 2_3:+63522525 Msa0305620:CDS
AAGGTACTACTGGACTAATT+TGG 0.361772 2_3:-63523019 None:intergenic
AAGTCAAATGTTCTATAAAC+AGG 0.392019 2_3:+63523268 Msa0305620:three_prime_UTR
TTCTTCTTCACTGTGCAGCT+TGG 0.402986 2_3:-63522888 None:intergenic
AGCATTTGAAGTATGCTCTT+GGG 0.421783 2_3:-63522857 None:intergenic
TTCCTACAACGATTGTTTGT+CGG 0.422479 2_3:-63522996 None:intergenic
CAAGTTTCTACTATTTGCCT+TGG 0.425526 2_3:-63522610 None:intergenic
GGGCTTTGCTCCTTATGATC+AGG 0.429712 2_3:-63522475 None:intergenic
GAGAAAGAATCCTGATCATA+AGG 0.436763 2_3:+63522465 Msa0305620:CDS
AGCAGCAGTAACAATCTAAT+GGG 0.440609 2_3:+63522526 Msa0305620:CDS
TCAGTCAAGTGGTGCCTTGG+TGG 0.446693 2_3:+63522339 Msa0305620:intron
GAGCATTTGAAGTATGCTCT+TGG 0.457888 2_3:-63522858 None:intergenic
ATCATGGATTAACACTTCCT+TGG 0.460598 2_3:+63522078 Msa0305620:CDS
GTGGAGAAAATATGGACAAA+AGG 0.461765 2_3:+63522815 Msa0305620:CDS
TTGAAGTATGCTCTTGGGGA+TGG 0.464325 2_3:-63522852 None:intergenic
TTCTTAGAAGGTTCAGAAGA+AGG 0.464649 2_3:-63523086 None:intergenic
AGCAAGGAAAAGAACTAGCT+TGG 0.467320 2_3:+63523455 Msa0305620:three_prime_UTR
CAATCAAGTGTAACTGTACT+TGG 0.475314 2_3:-63523050 None:intergenic
AGCAAAGCCCATCTCAGAAA+AGG 0.487615 2_3:+63522488 Msa0305620:CDS
GTCAAGTGGTGCCTTGGTGG+AGG 0.490012 2_3:+63522342 Msa0305620:intron
CTACTTAATGAATCAAAATG+AGG 0.491460 2_3:-63523357 None:intergenic
TGCTCTGAAGTTAAGATCCT+TGG 0.493603 2_3:-63523177 None:intergenic
TTTGATTCATTAAGTAGGAA+AGG 0.506007 2_3:+63523363 Msa0305620:three_prime_UTR
TCATCCTCATCCTTCTCAAA+CGG 0.516116 2_3:+63522971 Msa0305620:CDS
GCAGAAAACAAGAAGTTGAC+AGG 0.521855 2_3:+63522382 Msa0305620:CDS
TATGTTGCAACCACAACTGA+TGG 0.530178 2_3:-63522933 None:intergenic
CACATTGAGAAACAATCTGA+TGG 0.530744 2_3:+63522435 Msa0305620:CDS
GGTGCAGAAGAAGGTACTAC+TGG 0.531405 2_3:-63523029 None:intergenic
TGTAAGCTTCAAGAAGTATG+AGG 0.535043 2_3:+63522144 Msa0305620:CDS
ACTGTACTTGGTGCAGAAGA+AGG 0.542764 2_3:-63523038 None:intergenic
CAACATTAATCCACGCAGTA+GGG 0.544477 2_3:+63522105 Msa0305620:CDS
TCAACATTAATCCACGCAGT+AGG 0.551260 2_3:+63522104 Msa0305620:CDS
GTTGTGGTTGCAACATATGA+AGG 0.552701 2_3:+63522939 Msa0305620:CDS
AGCCGACAAACAATCGTTGT+AGG 0.557166 2_3:+63522994 Msa0305620:CDS
TATATCTTCTGCAAAAGAAG+AGG 0.565925 2_3:+63522198 Msa0305620:CDS
TTTGTCGGCTCCGTTTGAGA+AGG 0.566050 2_3:-63522981 None:intergenic
GGAAAAGAACTAGCTTGGAT+AGG 0.582825 2_3:+63523460 Msa0305620:three_prime_UTR
AAATACAACATAGAGAAAGT+AGG 0.586245 2_3:+63523328 Msa0305620:three_prime_UTR
TGGATTAATGTTGAGATCCA+AGG 0.586656 2_3:-63522095 None:intergenic
GAAGAAGGTGCAAAGAAGTG+TGG 0.601905 2_3:+63522905 Msa0305620:CDS
ATATGGACAAAAGGTGACAA+GGG 0.608161 2_3:+63522824 Msa0305620:CDS
ATCAATCATGCAAGAAACCA+AGG 0.612418 2_3:+63522593 Msa0305620:CDS
GCATTTGAAGTATGCTCTTG+GGG 0.616672 2_3:-63522856 None:intergenic
TACTTCTTGAAGCTTACAGG+AGG 0.621579 2_3:-63522139 None:intergenic
AATATGGACAAAAGGTGACA+AGG 0.623618 2_3:+63522823 Msa0305620:CDS
ATCTGTTCCACCAAAATCTG+TGG 0.633715 2_3:-63523140 None:intergenic
GGCTCCGTTTGAGAAGGATG+AGG 0.643319 2_3:-63522975 None:intergenic
AAGCTTCAAGAAGTATGAGG+TGG 0.647098 2_3:+63522147 Msa0305620:CDS
TTGTGAAAGATGGATATCAG+TGG 0.648462 2_3:+63522796 Msa0305620:CDS
TGTGGATGATCCATCAGTTG+TGG 0.652865 2_3:+63522923 Msa0305620:CDS
AGCAGTAACAATCTAATGGG+AGG 0.658927 2_3:+63522529 Msa0305620:CDS
AAGCTGCACAGTGAAGAAGA+AGG 0.665099 2_3:+63522890 Msa0305620:CDS
GATGGCTACTTCATTGACCA+AGG 0.672567 2_3:+63523160 Msa0305620:CDS
TGTTCATGAACCCTACTGCG+TGG 0.674456 2_3:-63522115 None:intergenic
TCGTTTCAGCTCCTCCACCA+AGG 0.688042 2_3:-63522353 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGAATAATTTATTTGACATA+TGG + chr2_3:63522045-63522064 Msa0305620:exon 15.0%
!!! AAAACAATTTTTTCTCATTA+GGG + chr2_3:63522170-63522189 Msa0305620:CDS 15.0%
!! TGAATAATTTATTTGACATA+TGG + chr2_3:63522045-63522064 Msa0305620:exon 15.0%
!!! AAAACAATTTTTTCTCATTA+GGG + chr2_3:63522170-63522189 Msa0305620:CDS 15.0%
!!! GAAAACAATTTTTTCTCATT+AGG + chr2_3:63522169-63522188 Msa0305620:CDS 20.0%
!! TTATGAATACTAGATATATG+AGG + chr2_3:63522273-63522292 Msa0305620:intron 20.0%
!! TTTATCATCATATTCTTAGA+AGG - chr2_3:63523101-63523120 None:intergenic 20.0%
!!! TTTTCTACTTGAACTAATTT+TGG - chr2_3:63523238-63523257 None:intergenic 20.0%
!! TTTGCAAAAACATAAACAAT+TGG - chr2_3:63523399-63523418 None:intergenic 20.0%
!!! GAAAACAATTTTTTCTCATT+AGG + chr2_3:63522169-63522188 Msa0305620:CDS 20.0%
!! TTATGAATACTAGATATATG+AGG + chr2_3:63522273-63522292 Msa0305620:intron 20.0%
!! TTTATCATCATATTCTTAGA+AGG - chr2_3:63523101-63523120 None:intergenic 20.0%
!!! TTTTCTACTTGAACTAATTT+TGG - chr2_3:63523238-63523257 None:intergenic 20.0%
!! TTTGCAAAAACATAAACAAT+TGG - chr2_3:63523399-63523418 None:intergenic 20.0%
!! ATATGGATTTTTCATCATCA+TGG + chr2_3:63522062-63522081 Msa0305620:exon 25.0%
!! TACTAGATATATGAGGTTTT+TGG + chr2_3:63522280-63522299 Msa0305620:intron 25.0%
!! TATTGATATTTTCAGTCAAG+TGG + chr2_3:63522328-63522347 Msa0305620:intron 25.0%
! AATAGTAGAAACTTGCATTA+AGG + chr2_3:63522618-63522637 Msa0305620:CDS 25.0%
! GAAGAAAAATGAATCTTACA+AGG - chr2_3:63522690-63522709 None:intergenic 25.0%
!! TATTTTCAGATTGTGAAAGA+TGG + chr2_3:63522786-63522805 Msa0305620:intron 25.0%
! GATGATAAATAATCCCAAAA+TGG + chr2_3:63523112-63523131 Msa0305620:CDS 25.0%
! AAGTCAAATGTTCTATAAAC+AGG + chr2_3:63523268-63523287 Msa0305620:three_prime_UTR 25.0%
!!! AACAGGTCATTTTTAAAACA+TGG + chr2_3:63523285-63523304 Msa0305620:three_prime_UTR 25.0%
!!! TCATTTTTAAAACATGGCAT+AGG + chr2_3:63523291-63523310 Msa0305620:three_prime_UTR 25.0%
! AAATACAACATAGAGAAAGT+AGG + chr2_3:63523328-63523347 Msa0305620:three_prime_UTR 25.0%
! CTACTTAATGAATCAAAATG+AGG - chr2_3:63523360-63523379 None:intergenic 25.0%
!!! CTCATTTTGATTCATTAAGT+AGG + chr2_3:63523358-63523377 Msa0305620:three_prime_UTR 25.0%
! TTTGATTCATTAAGTAGGAA+AGG + chr2_3:63523363-63523382 Msa0305620:three_prime_UTR 25.0%
!!! TGTTTATGTTTTTGCAAATC+TGG + chr2_3:63523401-63523420 Msa0305620:three_prime_UTR 25.0%
!!! TATATCTTTTTCATCAAGCA+AGG + chr2_3:63523439-63523458 Msa0305620:three_prime_UTR 25.0%
!!! GTTGCTTTGTTTAGAAATTT+TGG - chr2_3:63523514-63523533 None:intergenic 25.0%
!! ATATGGATTTTTCATCATCA+TGG + chr2_3:63522062-63522081 Msa0305620:exon 25.0%
!! TACTAGATATATGAGGTTTT+TGG + chr2_3:63522280-63522299 Msa0305620:intron 25.0%
!! TATTGATATTTTCAGTCAAG+TGG + chr2_3:63522328-63522347 Msa0305620:intron 25.0%
! AATAGTAGAAACTTGCATTA+AGG + chr2_3:63522618-63522637 Msa0305620:CDS 25.0%
! GAAGAAAAATGAATCTTACA+AGG - chr2_3:63522690-63522709 None:intergenic 25.0%
!! TATTTTCAGATTGTGAAAGA+TGG + chr2_3:63522786-63522805 Msa0305620:intron 25.0%
! GATGATAAATAATCCCAAAA+TGG + chr2_3:63523112-63523131 Msa0305620:CDS 25.0%
! AAGTCAAATGTTCTATAAAC+AGG + chr2_3:63523268-63523287 Msa0305620:three_prime_UTR 25.0%
!!! AACAGGTCATTTTTAAAACA+TGG + chr2_3:63523285-63523304 Msa0305620:three_prime_UTR 25.0%
!!! TCATTTTTAAAACATGGCAT+AGG + chr2_3:63523291-63523310 Msa0305620:three_prime_UTR 25.0%
! AAATACAACATAGAGAAAGT+AGG + chr2_3:63523328-63523347 Msa0305620:three_prime_UTR 25.0%
! CTACTTAATGAATCAAAATG+AGG - chr2_3:63523360-63523379 None:intergenic 25.0%
!!! CTCATTTTGATTCATTAAGT+AGG + chr2_3:63523358-63523377 Msa0305620:three_prime_UTR 25.0%
! TTTGATTCATTAAGTAGGAA+AGG + chr2_3:63523363-63523382 Msa0305620:three_prime_UTR 25.0%
!!! TGTTTATGTTTTTGCAAATC+TGG + chr2_3:63523401-63523420 Msa0305620:three_prime_UTR 25.0%
!!! TATATCTTTTTCATCAAGCA+AGG + chr2_3:63523439-63523458 Msa0305620:three_prime_UTR 25.0%
!!! GTTGCTTTGTTTAGAAATTT+TGG - chr2_3:63523514-63523533 None:intergenic 25.0%
TATATCTTCTGCAAAAGAAG+AGG + chr2_3:63522198-63522217 Msa0305620:CDS 30.0%
AAAGAAGAGGTAAGTTAGTT+TGG + chr2_3:63522211-63522230 Msa0305620:intron 30.0%
! GTAAGTTAGTTTGGTTACTT+TGG + chr2_3:63522220-63522239 Msa0305620:intron 30.0%
ATTTGCAAAGCCATTTCATT+AGG + chr2_3:63522717-63522736 Msa0305620:intron 30.0%
AGTCATACAACCTAATGAAA+TGG - chr2_3:63522730-63522749 None:intergenic 30.0%
! GTGTAACATTTTTCCTGATA+TGG - chr2_3:63523216-63523235 None:intergenic 30.0%
TATATCTTCTGCAAAAGAAG+AGG + chr2_3:63522198-63522217 Msa0305620:CDS 30.0%
AAAGAAGAGGTAAGTTAGTT+TGG + chr2_3:63522211-63522230 Msa0305620:intron 30.0%
! GTAAGTTAGTTTGGTTACTT+TGG + chr2_3:63522220-63522239 Msa0305620:intron 30.0%
ATTTGCAAAGCCATTTCATT+AGG + chr2_3:63522717-63522736 Msa0305620:intron 30.0%
AGTCATACAACCTAATGAAA+TGG - chr2_3:63522730-63522749 None:intergenic 30.0%
! GTGTAACATTTTTCCTGATA+TGG - chr2_3:63523216-63523235 None:intergenic 30.0%
! ATCATGGATTAACACTTCCT+TGG + chr2_3:63522078-63522097 Msa0305620:CDS 35.0%
TGGATTAATGTTGAGATCCA+AGG - chr2_3:63522098-63522117 None:intergenic 35.0%
TCATACTTCTTGAAGCTTAC+AGG - chr2_3:63522145-63522164 None:intergenic 35.0%
! TGTAAGCTTCAAGAAGTATG+AGG + chr2_3:63522144-63522163 Msa0305620:CDS 35.0%
CACATTGAGAAACAATCTGA+TGG + chr2_3:63522435-63522454 Msa0305620:CDS 35.0%
GAGAAAGAATCCTGATCATA+AGG + chr2_3:63522465-63522484 Msa0305620:CDS 35.0%
!!! CAGATTTCCTTTTCTGAGAT+GGG - chr2_3:63522498-63522517 None:intergenic 35.0%
!! TCAGATTTCCTTTTCTGAGA+TGG - chr2_3:63522499-63522518 None:intergenic 35.0%
AAGCAGCAGTAACAATCTAA+TGG + chr2_3:63522525-63522544 Msa0305620:CDS 35.0%
AGCAGCAGTAACAATCTAAT+GGG + chr2_3:63522526-63522545 Msa0305620:CDS 35.0%
ATCAATCATGCAAGAAACCA+AGG + chr2_3:63522593-63522612 Msa0305620:CDS 35.0%
CAAGTTTCTACTATTTGCCT+TGG - chr2_3:63522613-63522632 None:intergenic 35.0%
TTGTGAAAGATGGATATCAG+TGG + chr2_3:63522796-63522815 Msa0305620:CDS 35.0%
GGATATCAGTGGAGAAAATA+TGG + chr2_3:63522807-63522826 Msa0305620:CDS 35.0%
GTGGAGAAAATATGGACAAA+AGG + chr2_3:63522815-63522834 Msa0305620:CDS 35.0%
AATATGGACAAAAGGTGACA+AGG + chr2_3:63522823-63522842 Msa0305620:CDS 35.0%
ATATGGACAAAAGGTGACAA+GGG + chr2_3:63522824-63522843 Msa0305620:CDS 35.0%
! AGCATTTGAAGTATGCTCTT+GGG - chr2_3:63522860-63522879 None:intergenic 35.0%
TTCCTACAACGATTGTTTGT+CGG - chr2_3:63522999-63523018 None:intergenic 35.0%
! AAGGTACTACTGGACTAATT+TGG - chr2_3:63523022-63523041 None:intergenic 35.0%
CAATCAAGTGTAACTGTACT+TGG - chr2_3:63523053-63523072 None:intergenic 35.0%
CAAGTACAGTTACACTTGAT+TGG + chr2_3:63523051-63523070 Msa0305620:CDS 35.0%
TTCTTAGAAGGTTCAGAAGA+AGG - chr2_3:63523089-63523108 None:intergenic 35.0%
! ATCTGTGGTACTTCCATTTT+GGG - chr2_3:63523128-63523147 None:intergenic 35.0%
! AATCTGTGGTACTTCCATTT+TGG - chr2_3:63523129-63523148 None:intergenic 35.0%
!!! AATGGAAGTACCACAGATTT+TGG + chr2_3:63523130-63523149 Msa0305620:CDS 35.0%
AAGAAGATGAAGTCTTTGTG+TGG - chr2_3:63521980-63521999 None:intergenic 35.0%
! ATCATGGATTAACACTTCCT+TGG + chr2_3:63522078-63522097 Msa0305620:CDS 35.0%
TGGATTAATGTTGAGATCCA+AGG - chr2_3:63522098-63522117 None:intergenic 35.0%
TCATACTTCTTGAAGCTTAC+AGG - chr2_3:63522145-63522164 None:intergenic 35.0%
! TGTAAGCTTCAAGAAGTATG+AGG + chr2_3:63522144-63522163 Msa0305620:CDS 35.0%
CACATTGAGAAACAATCTGA+TGG + chr2_3:63522435-63522454 Msa0305620:CDS 35.0%
GAGAAAGAATCCTGATCATA+AGG + chr2_3:63522465-63522484 Msa0305620:CDS 35.0%
!!! CAGATTTCCTTTTCTGAGAT+GGG - chr2_3:63522498-63522517 None:intergenic 35.0%
!! TCAGATTTCCTTTTCTGAGA+TGG - chr2_3:63522499-63522518 None:intergenic 35.0%
AAGCAGCAGTAACAATCTAA+TGG + chr2_3:63522525-63522544 Msa0305620:CDS 35.0%
AGCAGCAGTAACAATCTAAT+GGG + chr2_3:63522526-63522545 Msa0305620:CDS 35.0%
ATCAATCATGCAAGAAACCA+AGG + chr2_3:63522593-63522612 Msa0305620:CDS 35.0%
CAAGTTTCTACTATTTGCCT+TGG - chr2_3:63522613-63522632 None:intergenic 35.0%
TTGTGAAAGATGGATATCAG+TGG + chr2_3:63522796-63522815 Msa0305620:CDS 35.0%
GGATATCAGTGGAGAAAATA+TGG + chr2_3:63522807-63522826 Msa0305620:CDS 35.0%
GTGGAGAAAATATGGACAAA+AGG + chr2_3:63522815-63522834 Msa0305620:CDS 35.0%
AATATGGACAAAAGGTGACA+AGG + chr2_3:63522823-63522842 Msa0305620:CDS 35.0%
ATATGGACAAAAGGTGACAA+GGG + chr2_3:63522824-63522843 Msa0305620:CDS 35.0%
! AGCATTTGAAGTATGCTCTT+GGG - chr2_3:63522860-63522879 None:intergenic 35.0%
TTCCTACAACGATTGTTTGT+CGG - chr2_3:63522999-63523018 None:intergenic 35.0%
! AAGGTACTACTGGACTAATT+TGG - chr2_3:63523022-63523041 None:intergenic 35.0%
CAATCAAGTGTAACTGTACT+TGG - chr2_3:63523053-63523072 None:intergenic 35.0%
CAAGTACAGTTACACTTGAT+TGG + chr2_3:63523051-63523070 Msa0305620:CDS 35.0%
TTCTTAGAAGGTTCAGAAGA+AGG - chr2_3:63523089-63523108 None:intergenic 35.0%
! ATCTGTGGTACTTCCATTTT+GGG - chr2_3:63523128-63523147 None:intergenic 35.0%
! AATCTGTGGTACTTCCATTT+TGG - chr2_3:63523129-63523148 None:intergenic 35.0%
!!! AATGGAAGTACCACAGATTT+TGG + chr2_3:63523130-63523149 Msa0305620:CDS 35.0%
TCAACATTAATCCACGCAGT+AGG + chr2_3:63522104-63522123 Msa0305620:CDS 40.0%
CAACATTAATCCACGCAGTA+GGG + chr2_3:63522105-63522124 Msa0305620:CDS 40.0%
TACTTCTTGAAGCTTACAGG+AGG - chr2_3:63522142-63522161 None:intergenic 40.0%
! AAGCTTCAAGAAGTATGAGG+TGG + chr2_3:63522147-63522166 Msa0305620:CDS 40.0%
GCAGAAAACAAGAAGTTGAC+AGG + chr2_3:63522382-63522401 Msa0305620:CDS 40.0%
AGCAGTAACAATCTAATGGG+AGG + chr2_3:63522529-63522548 Msa0305620:CDS 40.0%
AATCTAATGGGAGGAGTAAC+TGG + chr2_3:63522538-63522557 Msa0305620:CDS 40.0%
! GCATTTGAAGTATGCTCTTG+GGG - chr2_3:63522859-63522878 None:intergenic 40.0%
! GAGCATTTGAAGTATGCTCT+TGG - chr2_3:63522861-63522880 None:intergenic 40.0%
TATGTTGCAACCACAACTGA+TGG - chr2_3:63522936-63522955 None:intergenic 40.0%
GTTGTGGTTGCAACATATGA+AGG + chr2_3:63522939-63522958 Msa0305620:CDS 40.0%
TCATCCTCATCCTTCTCAAA+CGG + chr2_3:63522971-63522990 Msa0305620:CDS 40.0%
ATCTGTTCCACCAAAATCTG+TGG - chr2_3:63523143-63523162 None:intergenic 40.0%
!! ACAGATTTTGGTGGAACAGA+TGG + chr2_3:63523142-63523161 Msa0305620:CDS 40.0%
!! TGCTCTGAAGTTAAGATCCT+TGG - chr2_3:63523180-63523199 None:intergenic 40.0%
AGCAAGGAAAAGAACTAGCT+TGG + chr2_3:63523455-63523474 Msa0305620:three_prime_UTR 40.0%
! GGAAAAGAACTAGCTTGGAT+AGG + chr2_3:63523460-63523479 Msa0305620:three_prime_UTR 40.0%
TCAACATTAATCCACGCAGT+AGG + chr2_3:63522104-63522123 Msa0305620:CDS 40.0%
CAACATTAATCCACGCAGTA+GGG + chr2_3:63522105-63522124 Msa0305620:CDS 40.0%
TACTTCTTGAAGCTTACAGG+AGG - chr2_3:63522142-63522161 None:intergenic 40.0%
! AAGCTTCAAGAAGTATGAGG+TGG + chr2_3:63522147-63522166 Msa0305620:CDS 40.0%
GCAGAAAACAAGAAGTTGAC+AGG + chr2_3:63522382-63522401 Msa0305620:CDS 40.0%
AGCAGTAACAATCTAATGGG+AGG + chr2_3:63522529-63522548 Msa0305620:CDS 40.0%
AATCTAATGGGAGGAGTAAC+TGG + chr2_3:63522538-63522557 Msa0305620:CDS 40.0%
! GCATTTGAAGTATGCTCTTG+GGG - chr2_3:63522859-63522878 None:intergenic 40.0%
! GAGCATTTGAAGTATGCTCT+TGG - chr2_3:63522861-63522880 None:intergenic 40.0%
TATGTTGCAACCACAACTGA+TGG - chr2_3:63522936-63522955 None:intergenic 40.0%
GTTGTGGTTGCAACATATGA+AGG + chr2_3:63522939-63522958 Msa0305620:CDS 40.0%
TCATCCTCATCCTTCTCAAA+CGG + chr2_3:63522971-63522990 Msa0305620:CDS 40.0%
ATCTGTTCCACCAAAATCTG+TGG - chr2_3:63523143-63523162 None:intergenic 40.0%
!! ACAGATTTTGGTGGAACAGA+TGG + chr2_3:63523142-63523161 Msa0305620:CDS 40.0%
!! TGCTCTGAAGTTAAGATCCT+TGG - chr2_3:63523180-63523199 None:intergenic 40.0%
AGCAAGGAAAAGAACTAGCT+TGG + chr2_3:63523455-63523474 Msa0305620:three_prime_UTR 40.0%
! GGAAAAGAACTAGCTTGGAT+AGG + chr2_3:63523460-63523479 Msa0305620:three_prime_UTR 40.0%
! TTTTCAGTCAAGTGGTGCCT+TGG + chr2_3:63522336-63522355 Msa0305620:intron 45.0%
AGCAAAGCCCATCTCAGAAA+AGG + chr2_3:63522488-63522507 Msa0305620:CDS 45.0%
TTGAAGTATGCTCTTGGGGA+TGG - chr2_3:63522855-63522874 None:intergenic 45.0%
TTCTTCTTCACTGTGCAGCT+TGG - chr2_3:63522891-63522910 None:intergenic 45.0%
AAGCTGCACAGTGAAGAAGA+AGG + chr2_3:63522890-63522909 Msa0305620:CDS 45.0%
! GAAGAAGGTGCAAAGAAGTG+TGG + chr2_3:63522905-63522924 Msa0305620:CDS 45.0%
TGTGGATGATCCATCAGTTG+TGG + chr2_3:63522923-63522942 Msa0305620:CDS 45.0%
AGCCGACAAACAATCGTTGT+AGG + chr2_3:63522994-63523013 Msa0305620:CDS 45.0%
!! ACTGTACTTGGTGCAGAAGA+AGG - chr2_3:63523041-63523060 None:intergenic 45.0%
!!! GGAAGTACCACAGATTTTGG+TGG + chr2_3:63523133-63523152 Msa0305620:CDS 45.0%
GATGGCTACTTCATTGACCA+AGG + chr2_3:63523160-63523179 Msa0305620:CDS 45.0%
! TTTTCAGTCAAGTGGTGCCT+TGG + chr2_3:63522336-63522355 Msa0305620:intron 45.0%
AGCAAAGCCCATCTCAGAAA+AGG + chr2_3:63522488-63522507 Msa0305620:CDS 45.0%
TTGAAGTATGCTCTTGGGGA+TGG - chr2_3:63522855-63522874 None:intergenic 45.0%
TTCTTCTTCACTGTGCAGCT+TGG - chr2_3:63522891-63522910 None:intergenic 45.0%
AAGCTGCACAGTGAAGAAGA+AGG + chr2_3:63522890-63522909 Msa0305620:CDS 45.0%
! GAAGAAGGTGCAAAGAAGTG+TGG + chr2_3:63522905-63522924 Msa0305620:CDS 45.0%
TGTGGATGATCCATCAGTTG+TGG + chr2_3:63522923-63522942 Msa0305620:CDS 45.0%
AGCCGACAAACAATCGTTGT+AGG + chr2_3:63522994-63523013 Msa0305620:CDS 45.0%
!! ACTGTACTTGGTGCAGAAGA+AGG - chr2_3:63523041-63523060 None:intergenic 45.0%
!!! GGAAGTACCACAGATTTTGG+TGG + chr2_3:63523133-63523152 Msa0305620:CDS 45.0%
GATGGCTACTTCATTGACCA+AGG + chr2_3:63523160-63523179 Msa0305620:CDS 45.0%
TGTTCATGAACCCTACTGCG+TGG - chr2_3:63522118-63522137 None:intergenic 50.0%
GGGCTTTGCTCCTTATGATC+AGG - chr2_3:63522478-63522497 None:intergenic 50.0%
!! TTTGTCGGCTCCGTTTGAGA+AGG - chr2_3:63522984-63523003 None:intergenic 50.0%
!! GGTGCAGAAGAAGGTACTAC+TGG - chr2_3:63523032-63523051 None:intergenic 50.0%
TGTTCATGAACCCTACTGCG+TGG - chr2_3:63522118-63522137 None:intergenic 50.0%
GGGCTTTGCTCCTTATGATC+AGG - chr2_3:63522478-63522497 None:intergenic 50.0%
!! TTTGTCGGCTCCGTTTGAGA+AGG - chr2_3:63522984-63523003 None:intergenic 50.0%
!! GGTGCAGAAGAAGGTACTAC+TGG - chr2_3:63523032-63523051 None:intergenic 50.0%
TCAGTCAAGTGGTGCCTTGG+TGG + chr2_3:63522339-63522358 Msa0305620:intron 55.0%
TCGTTTCAGCTCCTCCACCA+AGG - chr2_3:63522356-63522375 None:intergenic 55.0%
GGCTCCGTTTGAGAAGGATG+AGG - chr2_3:63522978-63522997 None:intergenic 55.0%
! GCACTTGCTGCTGCCATATC+AGG + chr2_3:63523200-63523219 Msa0305620:CDS 55.0%
TCAGTCAAGTGGTGCCTTGG+TGG + chr2_3:63522339-63522358 Msa0305620:intron 55.0%
TCGTTTCAGCTCCTCCACCA+AGG - chr2_3:63522356-63522375 None:intergenic 55.0%
GGCTCCGTTTGAGAAGGATG+AGG - chr2_3:63522978-63522997 None:intergenic 55.0%
! GCACTTGCTGCTGCCATATC+AGG + chr2_3:63523200-63523219 Msa0305620:CDS 55.0%
GTCAAGTGGTGCCTTGGTGG+AGG + chr2_3:63522342-63522361 Msa0305620:intron 60.0%
GTCAAGTGGTGCCTTGGTGG+AGG + chr2_3:63522342-63522361 Msa0305620:intron 60.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 63521980 63523546 63521980 ID=Msa0305620;Name=Msa0305620
chr2_3 mRNA 63521980 63523546 63521980 ID=Msa0305620-mRNA-1;Parent=Msa0305620;Name=Msa0305620-mRNA-1;_AED=0.07;_eAED=0.07;_QI=84|1|1|1|1|1|3|302|317
chr2_3 exon 63521980 63522219 63521980 ID=Msa0305620-mRNA-1:exon:21204;Parent=Msa0305620-mRNA-1
chr2_3 exon 63522343 63522690 63522343 ID=Msa0305620-mRNA-1:exon:21205;Parent=Msa0305620-mRNA-1
chr2_3 exon 63522795 63523546 63522795 ID=Msa0305620-mRNA-1:exon:21206;Parent=Msa0305620-mRNA-1
chr2_3 five_prime_UTR 63521980 63522063 63521980 ID=Msa0305620-mRNA-1:five_prime_utr;Parent=Msa0305620-mRNA-1
chr2_3 CDS 63522064 63522219 63522064 ID=Msa0305620-mRNA-1:cds;Parent=Msa0305620-mRNA-1
chr2_3 CDS 63522343 63522690 63522343 ID=Msa0305620-mRNA-1:cds;Parent=Msa0305620-mRNA-1
chr2_3 CDS 63522795 63523244 63522795 ID=Msa0305620-mRNA-1:cds;Parent=Msa0305620-mRNA-1
chr2_3 three_prime_UTR 63523245 63523546 63523245 ID=Msa0305620-mRNA-1:three_prime_utr;Parent=Msa0305620-mRNA-1
Gene Sequence

>Msa0305620

ATGGATTTTTCATCATCATGGATTAACACTTCCTTGGATCTCAACATTAATCCACGCAGTAGGGTTCATGAACAACCTCCTGTAAGCTTCAAGAAGTATGAGGTGGAAAACAATTTTTTCTCATTAGGGATGAGTATATCTTCTGCAAAAGAAGAGTCAAGTGGTGCCTTGGTGGAGGAGCTGAAACGAGTGAGTGCAGAAAACAAGAAGTTGACAGGAATGTTGACTGAGATGTGTGAGAATTACAACACATTGAGAAACAATCTGATGGAATATATGAGAAAGAATCCTGATCATAAGGAGCAAAGCCCATCTCAGAAAAGGAAATCTGATGAAATAAGCAGCAGTAACAATCTAATGGGAGGAGTAACTGGAAACAACTCTGAGAGCAGTTCAAGTGATGAAGATCAATCATGCAAGAAACCAAGGCAAATAGTAGAAACTTGCATTAAGGCTAAAGTTTCAAGAGTTTATTTCAAGACAGAATCATCTGATTCAAGCCTTATTGTGAAAGATGGATATCAGTGGAGAAAATATGGACAAAAGGTGACAAGGGATAATCCATCCCCAAGAGCATACTTCAAATGCTCATTTGCTCCAAGCTGCACAGTGAAGAAGAAGGTGCAAAGAAGTGTGGATGATCCATCAGTTGTGGTTGCAACATATGAAGGAGAGCATAATCATCCTCATCCTTCTCAAACGGAGCCGACAAACAATCGTTGTAGGAACCAAATTAGTCCAGTAGTACCTTCTTCTGCACCAAGTACAGTTACACTTGATTGGACAAAGAAAGCACCTTCTTCTGAACCTTCTAAGAATATGATGATAAATAATCCCAAAATGGAAGTACCACAGATTTTGGTGGAACAGATGGCTACTTCATTGACCAAGGATCTTAACTTCAGAGCAGCACTTGCTGCTGCCATATCAGGAAAAATGTTACACCAAAATTAG
Protein sequence

>Msa0305620

MDFSSSWINTSLDLNINPRSRVHEQPPVSFKKYEVENNFFSLGMSISSAKEESSGALVEELKRVSAENKKLTGMLTEMCENYNTLRNNLMEYMRKNPDHKEQSPSQKRKSDEISSSNNLMGGVTGNNSESSSSDEDQSCKKPRQIVETCIKAKVSRVYFKTESSDSSLIVKDGYQWRKYGQKVTRDNPSPRAYFKCSFAPSCTVKKKVQRSVDDPSVVVATYEGEHNHPHPSQTEPTNNRCRNQISPVVPSSAPSTVTLDWTKKAPSSEPSKNMMINNPKMEVPQILVEQMATSLTKDLNFRAALAAAISGKMLHQN*