Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0268890 | AET02655.1 | 59.794 | 97 | 2 | 1 | 15 | 74 | 1 | 97 | 9.37e-29 | 108 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0268890 | sp|B5LMP5|RK33_CICAR | 97.561 | 41 | 1 | 0 | 35 | 75 | 26 | 66 | 4.58e-24 | 88.6 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0268890 | A0A4V1E3D4 | 100.000 | 41 | 0 | 0 | 35 | 75 | 26 | 66 | 4.03e-22 | 90.1 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0268460 | Msa0268890 | 0.811222 | 7.798028e-51 | -8.615850e-47 |
| Msa0268680 | Msa0268890 | 0.834300 | 3.245606e-56 | -8.615850e-47 |
| Msa0268690 | Msa0268890 | 0.840489 | 8.432011e-58 | -8.615850e-47 |
| Msa0268890 | Msa0268900 | 0.810497 | 1.119496e-50 | -8.615850e-47 |
| Msa0268890 | Msa0269100 | 0.818021 | 2.435539e-52 | -8.615850e-47 |
| Msa0268890 | Msa0344240 | 0.803963 | 2.712857e-49 | -8.615850e-47 |
| Msa0268890 | Msa1086340 | 0.811723 | 6.070978e-51 | -8.615850e-47 |
| Msa0268890 | Msa1086410 | 0.832479 | 9.235372e-56 | -8.615850e-47 |
| Msa0268890 | Msa1086520 | 0.816124 | 6.500408e-52 | -8.615850e-47 |
| Msa0268890 | Msa1086690 | 0.817975 | 2.494714e-52 | -8.615850e-47 |
| Msa0268890 | Msa1087000 | 0.833018 | 6.784862e-56 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0268890 | MtrunA17_CPg0492781 | 97.561 | 41 | 1 | 0 | 35 | 75 | 26 | 66 | 2.62e-25 | 89.0 |
| Msa0268890 | MtrunA17_Chr4g0015791 | 97.561 | 41 | 1 | 0 | 35 | 75 | 26 | 66 | 2.62e-25 | 89.0 |
| Msa0268890 | MtrunA17_Chr7g0224991 | 95.122 | 41 | 2 | 0 | 35 | 75 | 9 | 49 | 3.80e-24 | 85.5 |
| Msa0268890 | MtrunA17_Chr8g0355601 | 95.000 | 40 | 2 | 0 | 35 | 74 | 26 | 65 | 5.53e-24 | 85.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0268890 | ATCG00640.1 | 87.805 | 41 | 5 | 0 | 35 | 75 | 26 | 66 | 5.68e-21 | 78.2 |
Find 11 sgRNAs with CRISPR-Local
Find 110 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGTGTTAATAAGGAATCT+AGG | 0.323026 | 2_2:+70430397 | Msa0268890:intron |
| AATCGTATGTTTGCAACAAA+AGG | 0.396841 | 2_2:-70430493 | None:intergenic |
| CACCAGTGGCAAGTACTCTA+TGG | 0.426592 | 2_2:+70429738 | Msa0268890:CDS |
| AAGTGTTAATAAGGAATCTA+GGG | 0.443788 | 2_2:+70430398 | Msa0268890:intron |
| ACACAATACACCTAGTCGAT+TGG | 0.524208 | 2_2:+70430452 | Msa0268890:CDS |
| GGCAAGTACTCTATGGTTCG+CGG | 0.527936 | 2_2:+70429745 | Msa0268890:CDS |
| ATCGTATGTTTGCAACAAAA+GGG | 0.538893 | 2_2:-70430492 | None:intergenic |
| TTTCTCAATTCCAATCGACT+AGG | 0.574270 | 2_2:-70430462 | None:intergenic |
| ATATCTCTCTGTGGCACCAG+TGG | 0.585814 | 2_2:+70429724 | Msa0268890:CDS |
| AACCATAGAGTACTTGCCAC+TGG | 0.586013 | 2_2:-70429740 | None:intergenic |
| AGTGTTAATAAGGAATCTAG+GGG | 0.667965 | 2_2:+70430399 | Msa0268890:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATAACTAGAATCAAAAAAA+GGG | - | chr2_2:70429826-70429845 | None:intergenic | 15.0% |
| !! | AGAGCAAATTAATTTAATAA+AGG | + | chr2_2:70429949-70429968 | Msa0268890:intron | 15.0% |
| !! | TTAATTTAATAAAGGTGATT+TGG | + | chr2_2:70429957-70429976 | Msa0268890:intron | 15.0% |
| !! | TATTATTAAATTCATAGCAA+TGG | - | chr2_2:70430041-70430060 | None:intergenic | 15.0% |
| !! | CTGTACTATATAAAATTAAT+TGG | + | chr2_2:70430196-70430215 | Msa0268890:intron | 15.0% |
| !! | AATAACTAGAATCAAAAAAA+GGG | - | chr2_2:70429826-70429845 | None:intergenic | 15.0% |
| !! | AGAGCAAATTAATTTAATAA+AGG | + | chr2_2:70429949-70429968 | Msa0268890:intron | 15.0% |
| !! | TTAATTTAATAAAGGTGATT+TGG | + | chr2_2:70429957-70429976 | Msa0268890:intron | 15.0% |
| !! | TATTATTAAATTCATAGCAA+TGG | - | chr2_2:70430041-70430060 | None:intergenic | 15.0% |
| !! | CTGTACTATATAAAATTAAT+TGG | + | chr2_2:70430196-70430215 | Msa0268890:intron | 15.0% |
| !!! | TTCGTTTTTTTCTTGTAATT+TGG | - | chr2_2:70429675-70429694 | None:intergenic | 20.0% |
| !! | AAATTACAAGAAAAAAACGA+AGG | + | chr2_2:70429674-70429693 | Msa0268890:CDS | 20.0% |
| !! | ATAACTAGAATCAAAAAAAG+GGG | - | chr2_2:70429825-70429844 | None:intergenic | 20.0% |
| !! | CAATAACTAGAATCAAAAAA+AGG | - | chr2_2:70429827-70429846 | None:intergenic | 20.0% |
| !!! | CTTTTTTTGATTCTAGTTAT+TGG | + | chr2_2:70429825-70429844 | Msa0268890:intron | 20.0% |
| !!! | AGAGGGATTTAATATTTTAT+TGG | - | chr2_2:70429880-70429899 | None:intergenic | 20.0% |
| !! | TTCTATTACAATTCCATTTA+TGG | - | chr2_2:70430072-70430091 | None:intergenic | 20.0% |
| !!! | GATATACAAAACTGTTATTT+TGG | + | chr2_2:70430354-70430373 | Msa0268890:intron | 20.0% |
| !!! | GTGATAAAAAAGTGTTAATA+AGG | + | chr2_2:70430389-70430408 | Msa0268890:intron | 20.0% |
| !!! | TTCGTTTTTTTCTTGTAATT+TGG | - | chr2_2:70429675-70429694 | None:intergenic | 20.0% |
| !! | AAATTACAAGAAAAAAACGA+AGG | + | chr2_2:70429674-70429693 | Msa0268890:CDS | 20.0% |
| !! | ATAACTAGAATCAAAAAAAG+GGG | - | chr2_2:70429825-70429844 | None:intergenic | 20.0% |
| !! | CAATAACTAGAATCAAAAAA+AGG | - | chr2_2:70429827-70429846 | None:intergenic | 20.0% |
| !!! | CTTTTTTTGATTCTAGTTAT+TGG | + | chr2_2:70429825-70429844 | Msa0268890:intron | 20.0% |
| !!! | AGAGGGATTTAATATTTTAT+TGG | - | chr2_2:70429880-70429899 | None:intergenic | 20.0% |
| !! | TTCTATTACAATTCCATTTA+TGG | - | chr2_2:70430072-70430091 | None:intergenic | 20.0% |
| !!! | GATATACAAAACTGTTATTT+TGG | + | chr2_2:70430354-70430373 | Msa0268890:intron | 20.0% |
| !!! | GTGATAAAAAAGTGTTAATA+AGG | + | chr2_2:70430389-70430408 | Msa0268890:intron | 20.0% |
| ! | AAAAAAAAAACGAACGAAGA+GGG | - | chr2_2:70429897-70429916 | None:intergenic | 25.0% |
| ! | AAAAAAAAAAACGAACGAAG+AGG | - | chr2_2:70429898-70429917 | None:intergenic | 25.0% |
| ! | TGGAATTGTAATAGAATACT+TGG | + | chr2_2:70430076-70430095 | Msa0268890:intron | 25.0% |
| ! | GGAATTGTAATAGAATACTT+GGG | + | chr2_2:70430077-70430096 | Msa0268890:intron | 25.0% |
| ! | AAGTGAATCAAAAAAACCAA+AGG | + | chr2_2:70430310-70430329 | Msa0268890:intron | 25.0% |
| !! | AAAGTGTTAATAAGGAATCT+AGG | + | chr2_2:70430397-70430416 | Msa0268890:intron | 25.0% |
| ! | AAGTGTTAATAAGGAATCTA+GGG | + | chr2_2:70430398-70430417 | Msa0268890:intron | 25.0% |
| ! | AAAAAAAAAACGAACGAAGA+GGG | - | chr2_2:70429897-70429916 | None:intergenic | 25.0% |
| ! | AAAAAAAAAAACGAACGAAG+AGG | - | chr2_2:70429898-70429917 | None:intergenic | 25.0% |
| ! | TGGAATTGTAATAGAATACT+TGG | + | chr2_2:70430076-70430095 | Msa0268890:intron | 25.0% |
| ! | GGAATTGTAATAGAATACTT+GGG | + | chr2_2:70430077-70430096 | Msa0268890:intron | 25.0% |
| ! | AAGTGAATCAAAAAAACCAA+AGG | + | chr2_2:70430310-70430329 | Msa0268890:intron | 25.0% |
| !! | AAAGTGTTAATAAGGAATCT+AGG | + | chr2_2:70430397-70430416 | Msa0268890:intron | 25.0% |
| ! | AAGTGTTAATAAGGAATCTA+GGG | + | chr2_2:70430398-70430417 | Msa0268890:intron | 25.0% |
| TTACAAGAAAAAAACGAAGG+AGG | + | chr2_2:70429677-70429696 | Msa0268890:CDS | 30.0% | |
| TCTAAAAACATATCTCTCTG+TGG | + | chr2_2:70429715-70429734 | Msa0268890:CDS | 30.0% | |
| ! | TTTGATTCTAGTTATTGGCA+CGG | + | chr2_2:70429830-70429849 | Msa0268890:intron | 30.0% |
| AAAAACTCCTAATTTCACTG+AGG | - | chr2_2:70429983-70430002 | None:intergenic | 30.0% | |
| TAATAGAGTGAGACCATAAA+TGG | + | chr2_2:70430056-70430075 | Msa0268890:intron | 30.0% | |
| ATTGTAATAGAATACTTGGG+CGG | + | chr2_2:70430080-70430099 | Msa0268890:intron | 30.0% | |
| TGAATCAAAAAAACCAAAGG+AGG | + | chr2_2:70430313-70430332 | Msa0268890:intron | 30.0% | |
| ! | GTTTTGTATATCTTTACCCT+TGG | - | chr2_2:70430346-70430365 | None:intergenic | 30.0% |
| AGTGTTAATAAGGAATCTAG+GGG | + | chr2_2:70430399-70430418 | Msa0268890:intron | 30.0% | |
| ATCGTATGTTTGCAACAAAA+GGG | - | chr2_2:70430495-70430514 | None:intergenic | 30.0% | |
| AATCGTATGTTTGCAACAAA+AGG | - | chr2_2:70430496-70430515 | None:intergenic | 30.0% | |
| TTACAAGAAAAAAACGAAGG+AGG | + | chr2_2:70429677-70429696 | Msa0268890:CDS | 30.0% | |
| TCTAAAAACATATCTCTCTG+TGG | + | chr2_2:70429715-70429734 | Msa0268890:CDS | 30.0% | |
| ! | TTTGATTCTAGTTATTGGCA+CGG | + | chr2_2:70429830-70429849 | Msa0268890:intron | 30.0% |
| AAAAACTCCTAATTTCACTG+AGG | - | chr2_2:70429983-70430002 | None:intergenic | 30.0% | |
| TAATAGAGTGAGACCATAAA+TGG | + | chr2_2:70430056-70430075 | Msa0268890:intron | 30.0% | |
| ATTGTAATAGAATACTTGGG+CGG | + | chr2_2:70430080-70430099 | Msa0268890:intron | 30.0% | |
| TGAATCAAAAAAACCAAAGG+AGG | + | chr2_2:70430313-70430332 | Msa0268890:intron | 30.0% | |
| ! | GTTTTGTATATCTTTACCCT+TGG | - | chr2_2:70430346-70430365 | None:intergenic | 30.0% |
| AGTGTTAATAAGGAATCTAG+GGG | + | chr2_2:70430399-70430418 | Msa0268890:intron | 30.0% | |
| ATCGTATGTTTGCAACAAAA+GGG | - | chr2_2:70430495-70430514 | None:intergenic | 30.0% | |
| AATCGTATGTTTGCAACAAA+AGG | - | chr2_2:70430496-70430515 | None:intergenic | 30.0% | |
| ! | TGAAATTAGGAGTTTTTCCC+AGG | + | chr2_2:70429986-70430005 | Msa0268890:intron | 35.0% |
| CTTCAATTCCATTGAAACCT+GGG | - | chr2_2:70430006-70430025 | None:intergenic | 35.0% | |
| ACTTCAATTCCATTGAAACC+TGG | - | chr2_2:70430007-70430026 | None:intergenic | 35.0% | |
| TTGTAATAGAATACTTGGGC+GGG | + | chr2_2:70430081-70430100 | Msa0268890:intron | 35.0% | |
| TTCGAAGCGAAGAAGAAAAA+AGG | - | chr2_2:70430274-70430293 | None:intergenic | 35.0% | |
| AAAAAACCAAAGGAGGTTCA+TGG | + | chr2_2:70430320-70430339 | Msa0268890:intron | 35.0% | |
| TTTCTCAATTCCAATCGACT+AGG | - | chr2_2:70430465-70430484 | None:intergenic | 35.0% | |
| ! | TGAAATTAGGAGTTTTTCCC+AGG | + | chr2_2:70429986-70430005 | Msa0268890:intron | 35.0% |
| CTTCAATTCCATTGAAACCT+GGG | - | chr2_2:70430006-70430025 | None:intergenic | 35.0% | |
| ACTTCAATTCCATTGAAACC+TGG | - | chr2_2:70430007-70430026 | None:intergenic | 35.0% | |
| TTGTAATAGAATACTTGGGC+GGG | + | chr2_2:70430081-70430100 | Msa0268890:intron | 35.0% | |
| TTCGAAGCGAAGAAGAAAAA+AGG | - | chr2_2:70430274-70430293 | None:intergenic | 35.0% | |
| AAAAAACCAAAGGAGGTTCA+TGG | + | chr2_2:70430320-70430339 | Msa0268890:intron | 35.0% | |
| TTTCTCAATTCCAATCGACT+AGG | - | chr2_2:70430465-70430484 | None:intergenic | 35.0% | |
| AAAGGGGAATGTCAATGCAT+CGG | - | chr2_2:70429809-70429828 | None:intergenic | 40.0% | |
| GATTTGGCCTCAGTGAAATT+AGG | + | chr2_2:70429973-70429992 | Msa0268890:intron | 40.0% | |
| ! | GAGTTTTTCCCAGGTTTCAA+TGG | + | chr2_2:70429995-70430014 | Msa0268890:intron | 40.0% |
| ACACAATACACCTAGTCGAT+TGG | + | chr2_2:70430452-70430471 | Msa0268890:CDS | 40.0% | |
| AAAGGGGAATGTCAATGCAT+CGG | - | chr2_2:70429809-70429828 | None:intergenic | 40.0% | |
| GATTTGGCCTCAGTGAAATT+AGG | + | chr2_2:70429973-70429992 | Msa0268890:intron | 40.0% | |
| ! | GAGTTTTTCCCAGGTTTCAA+TGG | + | chr2_2:70429995-70430014 | Msa0268890:intron | 40.0% |
| ACACAATACACCTAGTCGAT+TGG | + | chr2_2:70430452-70430471 | Msa0268890:CDS | 40.0% | |
| ! | AACCATAGAGTACTTGCCAC+TGG | - | chr2_2:70429743-70429762 | None:intergenic | 45.0% |
| AAGGGGAATGTCAATGCATC+GGG | - | chr2_2:70429808-70429827 | None:intergenic | 45.0% | |
| ! | TTCTAGTTATTGGCACGGCA+AGG | + | chr2_2:70429835-70429854 | Msa0268890:intron | 45.0% |
| TCTAGTTATTGGCACGGCAA+GGG | + | chr2_2:70429836-70429855 | Msa0268890:intron | 45.0% | |
| ! | AACCATAGAGTACTTGCCAC+TGG | - | chr2_2:70429743-70429762 | None:intergenic | 45.0% |
| AAGGGGAATGTCAATGCATC+GGG | - | chr2_2:70429808-70429827 | None:intergenic | 45.0% | |
| ! | TTCTAGTTATTGGCACGGCA+AGG | + | chr2_2:70429835-70429854 | Msa0268890:intron | 45.0% |
| TCTAGTTATTGGCACGGCAA+GGG | + | chr2_2:70429836-70429855 | Msa0268890:intron | 45.0% | |
| ATATCTCTCTGTGGCACCAG+TGG | + | chr2_2:70429724-70429743 | Msa0268890:CDS | 50.0% | |
| CACCAGTGGCAAGTACTCTA+TGG | + | chr2_2:70429738-70429757 | Msa0268890:CDS | 50.0% | |
| GGCAAGTACTCTATGGTTCG+CGG | + | chr2_2:70429745-70429764 | Msa0268890:CDS | 50.0% | |
| CTAGTTATTGGCACGGCAAG+GGG | + | chr2_2:70429837-70429856 | Msa0268890:intron | 50.0% | |
| CAAAGGAGGTTCATGGCCAA+GGG | + | chr2_2:70430327-70430346 | Msa0268890:intron | 50.0% | |
| ATATCTCTCTGTGGCACCAG+TGG | + | chr2_2:70429724-70429743 | Msa0268890:CDS | 50.0% | |
| CACCAGTGGCAAGTACTCTA+TGG | + | chr2_2:70429738-70429757 | Msa0268890:CDS | 50.0% | |
| GGCAAGTACTCTATGGTTCG+CGG | + | chr2_2:70429745-70429764 | Msa0268890:CDS | 50.0% | |
| CTAGTTATTGGCACGGCAAG+GGG | + | chr2_2:70429837-70429856 | Msa0268890:intron | 50.0% | |
| CAAAGGAGGTTCATGGCCAA+GGG | + | chr2_2:70430327-70430346 | Msa0268890:intron | 50.0% | |
| ! | CTATGGTTCGCGGTTCTAGC+AGG | + | chr2_2:70429755-70429774 | Msa0268890:intron | 55.0% |
| CCTTGGCCATGAACCTCCTT+TGG | - | chr2_2:70430329-70430348 | None:intergenic | 55.0% | |
| CCAAAGGAGGTTCATGGCCA+AGG | + | chr2_2:70430326-70430345 | Msa0268890:intron | 55.0% | |
| ! | CTATGGTTCGCGGTTCTAGC+AGG | + | chr2_2:70429755-70429774 | Msa0268890:intron | 55.0% |
| CCTTGGCCATGAACCTCCTT+TGG | - | chr2_2:70430329-70430348 | None:intergenic | 55.0% | |
| CCAAAGGAGGTTCATGGCCA+AGG | + | chr2_2:70430326-70430345 | Msa0268890:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_2 | gene | 70429665 | 70430536 | 70429665 | ID=Msa0268890;Name=Msa0268890 |
| chr2_2 | mRNA | 70429665 | 70430536 | 70429665 | ID=Msa0268890-mRNA-1;Parent=Msa0268890;Name=Msa0268890-mRNA-1;_AED=0.51;_eAED=0.53;_QI=0|0|0|1|0|0|2|0|75 |
| chr2_2 | exon | 70429665 | 70429766 | 70429665 | ID=Msa0268890-mRNA-1:exon:22696;Parent=Msa0268890-mRNA-1 |
| chr2_2 | exon | 70430411 | 70430536 | 70430411 | ID=Msa0268890-mRNA-1:exon:22697;Parent=Msa0268890-mRNA-1 |
| chr2_2 | CDS | 70429665 | 70429766 | 70429665 | ID=Msa0268890-mRNA-1:cds;Parent=Msa0268890-mRNA-1 |
| chr2_2 | CDS | 70430411 | 70430536 | 70430411 | ID=Msa0268890-mRNA-1:cds;Parent=Msa0268890-mRNA-1 |
| Gene Sequence |
| Protein sequence |
