AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1087000


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1087000 MtrunA17_Chr4g0015801 81.301 123 10 3 73 182 1 123 3.22e-50 158
Msa1087000 MtrunA17_CPg0492771 81.301 123 10 3 73 182 1 123 3.22e-50 158
Msa1087000 MtrunA17_Chr6g0466591 88.889 90 6 1 73 158 1 90 2.30e-48 153
Msa1087000 MtrunA17_Chr4g0016891 86.957 92 8 2 73 160 1 92 2.54e-45 144
Msa1087000 MtrunA17_Chr7g0237831 94.667 75 2 1 73 145 1 75 4.69e-41 133
Msa1087000 MtrunA17_CPg0492781 98.361 61 1 0 1 61 1 61 6.23e-40 130
Msa1087000 MtrunA17_Chr4g0015791 98.361 61 1 0 1 61 1 61 6.23e-40 130
Msa1087000 MtrunA17_Chr8g0355601 96.721 61 2 0 1 61 1 61 3.20e-39 128
Msa1087000 MtrunA17_Chr6g0465781 96.610 59 2 0 73 131 1 59 2.39e-33 113
Msa1087000 MtrunA17_Chr7g0224991 88.095 42 5 0 19 60 2 43 9.70e-22 83.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1087000 ATCG00650.1 70.909 110 23 2 73 182 1 101 1.66e-43 140
Msa1087000 ATCG00640.1 77.049 61 14 0 1 61 1 61 1.48e-31 109

Find 19 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATACGAATAACTGTTATTT+TGG 0.125276 7_2:+87661919 Msa1087000:CDS
AGAGTGTTAATAAGGAATCT+AGG 0.329597 7_2:+87661963 Msa1087000:CDS
AATCGTATGTTTGCAACAAA+AGG 0.396841 7_2:-87662059 None:intergenic
GAGTGTTAATAAGGAATCTA+GGG 0.452009 7_2:+87661964 Msa1087000:CDS
AGTTTGTCCCCGATCCAATC+GGG 0.455483 7_2:+87662359 Msa1087000:CDS
GAGTTTGTCCCCGATCCAAT+CGG 0.465376 7_2:+87662358 Msa1087000:CDS
TATTCGTATATCTTTACCCT+TGG 0.471315 7_2:-87661906 None:intergenic
CCAAAGGAGGTTCATGGCCA+AGG 0.512674 7_2:+87661889 None:intergenic
AAAGGTGACGAATTAACAAA+AGG 0.521079 7_2:-87662533 None:intergenic
ACACAATACACCTAGTCGAT+TGG 0.524208 7_2:+87662018 Msa1087000:CDS
ATCGTATGTTTGCAACAAAA+GGG 0.538893 7_2:-87662058 None:intergenic
TTTCTCAATTCCAATCGACT+AGG 0.574270 7_2:-87662028 None:intergenic
GAGTCGCTTAGATTTATCCA+TGG 0.585856 7_2:-87662306 None:intergenic
CAAAGGAGGTTCATGGCCAA+GGG 0.605158 7_2:+87661890 None:intergenic
GTATAGGAATAAACAAACCA+TGG 0.629025 7_2:+87662289 Msa1087000:CDS
TATCGACTTATTAGTCAACA+AGG 0.637963 7_2:+87662416 Msa1087000:CDS
GTTTGTCCCCGATCCAATCG+GGG 0.647747 7_2:+87662360 Msa1087000:CDS
TTTGTCCCCGATCCAATCGG+GGG 0.661952 7_2:+87662361 Msa1087000:CDS
AGTGTTAATAAGGAATCTAG+GGG 0.667965 7_2:+87661965 Msa1087000:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATCCTTTTTTTATAAGAAA+TGG + chr7_2:87662258-87662277 Msa1087000:intron 15.0%
!!! ATCCTTTTTTTATAAGAAAT+GGG + chr7_2:87662259-87662278 Msa1087000:intron 15.0%
!!! TTTTTTTCATTATTCACAAA+AGG - chr7_2:87662554-87662573 None:intergenic 15.0%
!!! TATACGAATAACTGTTATTT+TGG + chr7_2:87661919-87661938 Msa1087000:CDS 20.0%
!!! TGATAAAAAGAGTGTTAATA+AGG + chr7_2:87661955-87661974 Msa1087000:CDS 20.0%
!! AGATAAAGAAATAGAGAAAA+TGG + chr7_2:87662089-87662108 Msa1087000:intron 20.0%
!! ATCCCATTTCTTATAAAAAA+AGG - chr7_2:87662264-87662283 None:intergenic 20.0%
!!! TTTATAAGAAATGGGATTTT+CGG + chr7_2:87662267-87662286 Msa1087000:intron 20.0%
!!! AATTAATCGTTGTTGTTTTA+AGG - chr7_2:87662474-87662493 None:intergenic 20.0%
!!! TTTTTTTTTAGAACAGTAGT+AGG - chr7_2:87662611-87662630 None:intergenic 20.0%
! TTATAGAATTTACCTCCTAA+GGG - chr7_2:87662130-87662149 None:intergenic 25.0%
! TTTATAGAATTTACCTCCTA+AGG - chr7_2:87662131-87662150 None:intergenic 25.0%
TATTCGTATATCTTTACCCT+TGG - chr7_2:87661909-87661928 None:intergenic 30.0%
! AGAGTGTTAATAAGGAATCT+AGG + chr7_2:87661963-87661982 Msa1087000:CDS 30.0%
GAGTGTTAATAAGGAATCTA+GGG + chr7_2:87661964-87661983 Msa1087000:CDS 30.0%
AGTGTTAATAAGGAATCTAG+GGG + chr7_2:87661965-87661984 Msa1087000:CDS 30.0%
ATCGTATGTTTGCAACAAAA+GGG - chr7_2:87662061-87662080 None:intergenic 30.0%
AATCGTATGTTTGCAACAAA+AGG - chr7_2:87662062-87662081 None:intergenic 30.0%
GTATAGGAATAAACAAACCA+TGG + chr7_2:87662289-87662308 Msa1087000:CDS 30.0%
! AATCTAAGCAATCTTTTCGT+AGG + chr7_2:87662336-87662355 Msa1087000:CDS 30.0%
!! TATCGACTTATTAGTCAACA+AGG + chr7_2:87662416-87662435 Msa1087000:CDS 30.0%
AAAGGTGACGAATTAACAAA+AGG - chr7_2:87662536-87662555 None:intergenic 30.0%
TTTCTCAATTCCAATCGACT+AGG - chr7_2:87662031-87662050 None:intergenic 35.0%
! AGAAATGGGATTTTCGGTAT+AGG + chr7_2:87662273-87662292 Msa1087000:intron 35.0%
ACACAATACACCTAGTCGAT+TGG + chr7_2:87662018-87662037 Msa1087000:CDS 40.0%
ATTGTTTGTGTGTCACCCTT+AGG + chr7_2:87662112-87662131 Msa1087000:intron 40.0%
GAGTCGCTTAGATTTATCCA+TGG - chr7_2:87662309-87662328 None:intergenic 40.0%
AATCAATTCGATCCCCCGAT+TGG - chr7_2:87662376-87662395 None:intergenic 45.0%
!!! ATATTTATATTTGTTATATA+AGG - chr7_2:87662203-87662222 None:intergenic 5.0%
GTTTGTGTGTCACCCTTAGG+AGG + chr7_2:87662115-87662134 Msa1087000:intron 50.0%
GAGTTTGTCCCCGATCCAAT+CGG + chr7_2:87662358-87662377 Msa1087000:CDS 50.0%
AGTTTGTCCCCGATCCAATC+GGG + chr7_2:87662359-87662378 Msa1087000:CDS 50.0%
ATTCGATCCCCCGATTGGAT+CGG - chr7_2:87662371-87662390 None:intergenic 50.0%
GTTTGTCCCCGATCCAATCG+GGG + chr7_2:87662360-87662379 Msa1087000:CDS 55.0%
TTTGTCCCCGATCCAATCGG+GGG + chr7_2:87662361-87662380 Msa1087000:CDS 55.0%
TTCGATCCCCCGATTGGATC+GGG - chr7_2:87662370-87662389 None:intergenic 55.0%
TCGATCCCCCGATTGGATCG+GGG - chr7_2:87662369-87662388 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr7_2 gene 87661902 87662640 87661902 ID=Msa1087000;Name=Msa1087000
chr7_2 mRNA 87661902 87662640 87661902 ID=Msa1087000-mRNA-1;Parent=Msa1087000;Name=Msa1087000-mRNA-1;_AED=0.22;_eAED=0.22;_QI=0|0|0|1|0|0|2|0|182
chr7_2 exon 87661902 87662084 87661902 ID=Msa1087000-mRNA-1:exon:23944;Parent=Msa1087000-mRNA-1
chr7_2 exon 87662275 87662640 87662275 ID=Msa1087000-mRNA-1:exon:23945;Parent=Msa1087000-mRNA-1
chr7_2 CDS 87661902 87662084 87661902 ID=Msa1087000-mRNA-1:cds;Parent=Msa1087000-mRNA-1
chr7_2 CDS 87662275 87662640 87662275 ID=Msa1087000-mRNA-1:cds;Parent=Msa1087000-mRNA-1
Gene Sequence

>Msa1087000

ATGGCCAAGGGTAAAGATATACGAATAACTGTTATTTTGGAATGTAGCAGTTGTGATAAAAAGAGTGTTAATAAGGAATCTAGGGGTATTTCTAGATATATTACTCAAAAGAATCGACACAATACACCTAGTCGATTGGAATTGAGAAAATTCTGTCCCTTTTGTTGCAAACATACGATTCACAAATGGGATTTTCGGTATAGGAATAAACAAACCATGGATAAATCTAAGCGACTCTTTCTTAAATCTAAGCAATCTTTTCGTAGGAGTTTGTCCCCGATCCAATCGGGGGATCGAATTGATTATAAAAACATGAGTTTACTTTATCGACTTATTAGTCAACAAGGAAAAATATTATCTAGACGCGTGAATAGATCAACCTTAAAACAACAACGATTAATTACGATTGCTATAAAACAAGCTCGTATTTTATCTTTGTTACCTTTTGTTAATTCGTCACCTTTTGTGAATAATGAAAAAAAAAAATATGAAAAAAGCGAGTCGATCACTAGAACTCCTACTACTGTTCTAAAAAAAAACAAAAAATAG

Protein sequence

>Msa1087000

MAKGKDIRITVILECSSCDKKSVNKESRGISRYITQKNRHNTPSRLELRKFCPFCCKHTIHKWDFRYRNKQTMDKSKRLFLKSKQSFRRSLSPIQSGDRIDYKNMSLLYRLISQQGKILSRRVNRSTLKQQRLITIAIKQARILSLLPFVNSSPFVNNEKKKYEKSESITRTPTTVLKKNKK*