AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0268690


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0268690 MtrunA17_CPg0492781 98.333 60 1 0 46 105 7 66 1.14e-38 124
Msa0268690 MtrunA17_Chr4g0015791 98.333 60 1 0 46 105 7 66 1.14e-38 124
Msa0268690 MtrunA17_Chr8g0355601 96.610 59 2 0 46 104 7 65 1.73e-37 120
Msa0268690 MtrunA17_Chr7g0224991 95.455 44 2 0 62 105 6 49 7.95e-26 90.9
Msa0268690 MtrunA17_CPg0492791 93.750 32 2 0 15 46 1 32 2.06e-15 64.3
Msa0268690 MtrunA17_Chr4g0015781 93.750 32 2 0 15 46 1 32 2.06e-15 64.3
Msa0268690 MtrunA17_Chr4g0028951 93.750 32 2 0 15 46 1 32 2.06e-15 64.3
Msa0268690 MtrunA17_Chr8g0355611 93.750 32 2 0 15 46 1 32 2.06e-15 64.3
Msa0268690 MtrunA17_Chr4g0016881 96.774 31 1 0 15 45 1 31 5.83e-15 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0268690 ATCG00640.1 75.000 60 15 0 46 105 7 66 2.13e-29 100
Msa0268690 ATCG00630.1 84.375 32 5 0 15 46 1 32 8.57e-13 58.2

Find 13 sgRNAs with CRISPR-Local

Find 110 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATACGAATAACTGTTATTT+TGG 0.125276 2_2:-70368912 Msa0268690:CDS
AGAGTGTTAATAAGGAATCT+AGG 0.329597 2_2:-70368868 Msa0268690:CDS
AATCGTATGTTTGCAACAAA+AGG 0.396841 2_2:+70368772 None:intergenic
CACCAGTGGCAAGTACTCTA+TGG 0.426592 2_2:-70369534 Msa0268690:CDS
GAGTGTTAATAAGGAATCTA+GGG 0.452009 2_2:-70368867 Msa0268690:CDS
CTATGGTTCGCGGTTCTAGC+AGG 0.487956 2_2:-70369517 Msa0268690:CDS
ACACAATACACCTAGTCGAT+TGG 0.524208 2_2:-70368813 Msa0268690:CDS
GGCAAGTACTCTATGGTTCG+CGG 0.527936 2_2:-70369527 Msa0268690:CDS
ATCGTATGTTTGCAACAAAA+GGG 0.538893 2_2:+70368773 None:intergenic
TTTCTCAATTCCAATCGACT+AGG 0.574270 2_2:+70368803 None:intergenic
ATATCTCTCTGTGGCACCAG+TGG 0.585814 2_2:-70369548 Msa0268690:CDS
AACCATAGAGTACTTGCCAC+TGG 0.586013 2_2:+70369532 None:intergenic
AGTGTTAATAAGGAATCTAG+GGG 0.667965 2_2:-70368866 Msa0268690:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAACTAGAATCAAAAAAA+GGG + chr2_2:70368912-70368931 None:intergenic 15.0%
!! AGAGCAAATTAATTTAATAA+AGG - chr2_2:70369035-70369054 Msa0268690:intron 15.0%
!! TATTATTAAATTCATAGCAA+TGG + chr2_2:70369129-70369148 None:intergenic 15.0%
!! CTGTACTATATAAAATTAAT+TGG - chr2_2:70369285-70369304 Msa0268690:intron 15.0%
!! AATAACTAGAATCAAAAAAA+GGG + chr2_2:70368912-70368931 None:intergenic 15.0%
!! AGAGCAAATTAATTTAATAA+AGG - chr2_2:70369035-70369054 Msa0268690:intron 15.0%
!! TATTATTAAATTCATAGCAA+TGG + chr2_2:70369129-70369148 None:intergenic 15.0%
!! CTGTACTATATAAAATTAAT+TGG - chr2_2:70369285-70369304 Msa0268690:intron 15.0%
!!! TTCGTTTTTTTCTTGTAATT+TGG + chr2_2:70368761-70368780 None:intergenic 20.0%
!! AAATTACAAGAAAAAAACGA+AGG - chr2_2:70368760-70368779 Msa0268690:CDS 20.0%
!! ATAACTAGAATCAAAAAAAG+GGG + chr2_2:70368911-70368930 None:intergenic 20.0%
!! CAATAACTAGAATCAAAAAA+AGG + chr2_2:70368913-70368932 None:intergenic 20.0%
!!! CTTTTTTTGATTCTAGTTAT+TGG - chr2_2:70368911-70368930 Msa0268690:CDS 20.0%
!!! AGAGGGATTTAATATTTTAT+TGG + chr2_2:70368966-70368985 None:intergenic 20.0%
!! GCAAATTAATTTAATAAAGG+TGG - chr2_2:70369038-70369057 Msa0268690:intron 20.0%
!!! TAATTTAATAAAGGTGGATT+TGG - chr2_2:70369044-70369063 Msa0268690:intron 20.0%
!! TTCTATTACAATTCCATTTA+TGG + chr2_2:70369160-70369179 None:intergenic 20.0%
!! AAGTGAATCAAAAAAAACAA+AGG - chr2_2:70369400-70369419 Msa0268690:intron 20.0%
!!! TATACGAATAACTGTTATTT+TGG - chr2_2:70369446-70369465 Msa0268690:intron 20.0%
!!! TGATAAAAAGAGTGTTAATA+AGG - chr2_2:70369482-70369501 Msa0268690:intron 20.0%
!!! TTCGTTTTTTTCTTGTAATT+TGG + chr2_2:70368761-70368780 None:intergenic 20.0%
!! AAATTACAAGAAAAAAACGA+AGG - chr2_2:70368760-70368779 Msa0268690:CDS 20.0%
!! ATAACTAGAATCAAAAAAAG+GGG + chr2_2:70368911-70368930 None:intergenic 20.0%
!! CAATAACTAGAATCAAAAAA+AGG + chr2_2:70368913-70368932 None:intergenic 20.0%
!!! CTTTTTTTGATTCTAGTTAT+TGG - chr2_2:70368911-70368930 Msa0268690:CDS 20.0%
!!! AGAGGGATTTAATATTTTAT+TGG + chr2_2:70368966-70368985 None:intergenic 20.0%
!! GCAAATTAATTTAATAAAGG+TGG - chr2_2:70369038-70369057 Msa0268690:intron 20.0%
!!! TAATTTAATAAAGGTGGATT+TGG - chr2_2:70369044-70369063 Msa0268690:intron 20.0%
!! TTCTATTACAATTCCATTTA+TGG + chr2_2:70369160-70369179 None:intergenic 20.0%
!! AAGTGAATCAAAAAAAACAA+AGG - chr2_2:70369400-70369419 Msa0268690:intron 20.0%
!!! TATACGAATAACTGTTATTT+TGG - chr2_2:70369446-70369465 Msa0268690:intron 20.0%
!!! TGATAAAAAGAGTGTTAATA+AGG - chr2_2:70369482-70369501 Msa0268690:intron 20.0%
! AAAAAAAAAACGAACGAAGA+GGG + chr2_2:70368983-70369002 None:intergenic 25.0%
! AAAAAAAAAAACGAACGAAG+AGG + chr2_2:70368984-70369003 None:intergenic 25.0%
! TGGAATTGTAATAGAATACT+TGG - chr2_2:70369164-70369183 Msa0268690:intron 25.0%
! GGAATTGTAATAGAATACTT+GGG - chr2_2:70369165-70369184 Msa0268690:intron 25.0%
! TGAATCAAAAAAAACAAAGG+AGG - chr2_2:70369403-70369422 Msa0268690:intron 25.0%
! AAAAAAAAAACGAACGAAGA+GGG + chr2_2:70368983-70369002 None:intergenic 25.0%
! AAAAAAAAAAACGAACGAAG+AGG + chr2_2:70368984-70369003 None:intergenic 25.0%
! TGGAATTGTAATAGAATACT+TGG - chr2_2:70369164-70369183 Msa0268690:intron 25.0%
! GGAATTGTAATAGAATACTT+GGG - chr2_2:70369165-70369184 Msa0268690:intron 25.0%
! TGAATCAAAAAAAACAAAGG+AGG - chr2_2:70369403-70369422 Msa0268690:intron 25.0%
TTACAAGAAAAAAACGAAGG+AGG - chr2_2:70368763-70368782 Msa0268690:CDS 30.0%
TCTAAAAACATATCTCTCTG+TGG - chr2_2:70368801-70368820 Msa0268690:CDS 30.0%
! TTTGATTCTAGTTATTGGCA+CGG - chr2_2:70368916-70368935 Msa0268690:CDS 30.0%
AAAAACTCCTAATTTCACTG+AGG + chr2_2:70369070-70369089 None:intergenic 30.0%
TAATAGAGTGAGACCATAAA+TGG - chr2_2:70369144-70369163 Msa0268690:intron 30.0%
ATTGTAATAGAATACTTGGG+CGG - chr2_2:70369168-70369187 Msa0268690:intron 30.0%
AAAAAAACAAAGGAGGTTCA+TGG - chr2_2:70369410-70369429 Msa0268690:intron 30.0%
TATTCGTATATCTTTACCCT+TGG + chr2_2:70369436-70369455 None:intergenic 30.0%
! AGAGTGTTAATAAGGAATCT+AGG - chr2_2:70369490-70369509 Msa0268690:intron 30.0%
GAGTGTTAATAAGGAATCTA+GGG - chr2_2:70369491-70369510 Msa0268690:intron 30.0%
AGTGTTAATAAGGAATCTAG+GGG - chr2_2:70369492-70369511 Msa0268690:intron 30.0%
ATCGTATGTTTGCAACAAAA+GGG + chr2_2:70369588-70369607 None:intergenic 30.0%
AATCGTATGTTTGCAACAAA+AGG + chr2_2:70369589-70369608 None:intergenic 30.0%
TTACAAGAAAAAAACGAAGG+AGG - chr2_2:70368763-70368782 Msa0268690:CDS 30.0%
TCTAAAAACATATCTCTCTG+TGG - chr2_2:70368801-70368820 Msa0268690:CDS 30.0%
! TTTGATTCTAGTTATTGGCA+CGG - chr2_2:70368916-70368935 Msa0268690:CDS 30.0%
AAAAACTCCTAATTTCACTG+AGG + chr2_2:70369070-70369089 None:intergenic 30.0%
TAATAGAGTGAGACCATAAA+TGG - chr2_2:70369144-70369163 Msa0268690:intron 30.0%
ATTGTAATAGAATACTTGGG+CGG - chr2_2:70369168-70369187 Msa0268690:intron 30.0%
AAAAAAACAAAGGAGGTTCA+TGG - chr2_2:70369410-70369429 Msa0268690:intron 30.0%
TATTCGTATATCTTTACCCT+TGG + chr2_2:70369436-70369455 None:intergenic 30.0%
! AGAGTGTTAATAAGGAATCT+AGG - chr2_2:70369490-70369509 Msa0268690:intron 30.0%
GAGTGTTAATAAGGAATCTA+GGG - chr2_2:70369491-70369510 Msa0268690:intron 30.0%
AGTGTTAATAAGGAATCTAG+GGG - chr2_2:70369492-70369511 Msa0268690:intron 30.0%
ATCGTATGTTTGCAACAAAA+GGG + chr2_2:70369588-70369607 None:intergenic 30.0%
AATCGTATGTTTGCAACAAA+AGG + chr2_2:70369589-70369608 None:intergenic 30.0%
! TGAAATTAGGAGTTTTTCCC+AGG - chr2_2:70369073-70369092 Msa0268690:intron 35.0%
CTTCAATTCCATTGAAACCT+GGG + chr2_2:70369093-70369112 None:intergenic 35.0%
ACTTCAATTCCATTGAAACC+TGG + chr2_2:70369094-70369113 None:intergenic 35.0%
TTGTAATAGAATACTTGGGC+GGG - chr2_2:70369169-70369188 Msa0268690:intron 35.0%
TTCGAAGCGAAGAAGAAAAA+AGG + chr2_2:70369364-70369383 None:intergenic 35.0%
TTTCTCAATTCCAATCGACT+AGG + chr2_2:70369558-70369577 None:intergenic 35.0%
! TGAAATTAGGAGTTTTTCCC+AGG - chr2_2:70369073-70369092 Msa0268690:intron 35.0%
CTTCAATTCCATTGAAACCT+GGG + chr2_2:70369093-70369112 None:intergenic 35.0%
ACTTCAATTCCATTGAAACC+TGG + chr2_2:70369094-70369113 None:intergenic 35.0%
TTGTAATAGAATACTTGGGC+GGG - chr2_2:70369169-70369188 Msa0268690:intron 35.0%
TTCGAAGCGAAGAAGAAAAA+AGG + chr2_2:70369364-70369383 None:intergenic 35.0%
TTTCTCAATTCCAATCGACT+AGG + chr2_2:70369558-70369577 None:intergenic 35.0%
AAAGGGGAATGTCAATGCAT+CGG + chr2_2:70368895-70368914 None:intergenic 40.0%
GATTTGGCCTCAGTGAAATT+AGG - chr2_2:70369060-70369079 Msa0268690:intron 40.0%
! GAGTTTTTCCCAGGTTTCAA+TGG - chr2_2:70369082-70369101 Msa0268690:intron 40.0%
ACACAATACACCTAGTCGAT+TGG - chr2_2:70369545-70369564 Msa0268690:CDS 40.0%
AAAGGGGAATGTCAATGCAT+CGG + chr2_2:70368895-70368914 None:intergenic 40.0%
GATTTGGCCTCAGTGAAATT+AGG - chr2_2:70369060-70369079 Msa0268690:intron 40.0%
! GAGTTTTTCCCAGGTTTCAA+TGG - chr2_2:70369082-70369101 Msa0268690:intron 40.0%
ACACAATACACCTAGTCGAT+TGG - chr2_2:70369545-70369564 Msa0268690:CDS 40.0%
! AACCATAGAGTACTTGCCAC+TGG + chr2_2:70368829-70368848 None:intergenic 45.0%
AAGGGGAATGTCAATGCATC+GGG + chr2_2:70368894-70368913 None:intergenic 45.0%
! TTCTAGTTATTGGCACGGCA+AGG - chr2_2:70368921-70368940 Msa0268690:intron 45.0%
TCTAGTTATTGGCACGGCAA+GGG - chr2_2:70368922-70368941 Msa0268690:intron 45.0%
! AACCATAGAGTACTTGCCAC+TGG + chr2_2:70368829-70368848 None:intergenic 45.0%
AAGGGGAATGTCAATGCATC+GGG + chr2_2:70368894-70368913 None:intergenic 45.0%
! TTCTAGTTATTGGCACGGCA+AGG - chr2_2:70368921-70368940 Msa0268690:intron 45.0%
TCTAGTTATTGGCACGGCAA+GGG - chr2_2:70368922-70368941 Msa0268690:intron 45.0%
ATATCTCTCTGTGGCACCAG+TGG - chr2_2:70368810-70368829 Msa0268690:CDS 50.0%
CACCAGTGGCAAGTACTCTA+TGG - chr2_2:70368824-70368843 Msa0268690:CDS 50.0%
GGCAAGTACTCTATGGTTCG+CGG - chr2_2:70368831-70368850 Msa0268690:CDS 50.0%
CTAGTTATTGGCACGGCAAG+GGG - chr2_2:70368923-70368942 Msa0268690:intron 50.0%
ACAAAGGAGGTTCATGGCCA+AGG - chr2_2:70369416-70369435 Msa0268690:intron 50.0%
CAAAGGAGGTTCATGGCCAA+GGG - chr2_2:70369417-70369436 Msa0268690:intron 50.0%
ATATCTCTCTGTGGCACCAG+TGG - chr2_2:70368810-70368829 Msa0268690:CDS 50.0%
CACCAGTGGCAAGTACTCTA+TGG - chr2_2:70368824-70368843 Msa0268690:CDS 50.0%
GGCAAGTACTCTATGGTTCG+CGG - chr2_2:70368831-70368850 Msa0268690:CDS 50.0%
CTAGTTATTGGCACGGCAAG+GGG - chr2_2:70368923-70368942 Msa0268690:intron 50.0%
ACAAAGGAGGTTCATGGCCA+AGG - chr2_2:70369416-70369435 Msa0268690:intron 50.0%
CAAAGGAGGTTCATGGCCAA+GGG - chr2_2:70369417-70369436 Msa0268690:intron 50.0%
! CTATGGTTCGCGGTTCTAGC+AGG - chr2_2:70368841-70368860 Msa0268690:CDS 55.0%
! CTATGGTTCGCGGTTCTAGC+AGG - chr2_2:70368841-70368860 Msa0268690:CDS 55.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 70368751 70369629 70368751 ID=Msa0268690;Name=Msa0268690
chr2_2 mRNA 70368751 70369629 70368751 ID=Msa0268690-mRNA-1;Parent=Msa0268690;Name=Msa0268690-mRNA-1;_AED=0.45;_eAED=0.45;_QI=0|0|0|1|0|0|2|0|105
chr2_2 exon 70369497 70369629 70369497 ID=Msa0268690-mRNA-1:exon:22599;Parent=Msa0268690-mRNA-1
chr2_2 exon 70368751 70368935 70368751 ID=Msa0268690-mRNA-1:exon:22598;Parent=Msa0268690-mRNA-1
chr2_2 CDS 70369497 70369629 70369497 ID=Msa0268690-mRNA-1:cds;Parent=Msa0268690-mRNA-1
chr2_2 CDS 70368751 70368935 70368751 ID=Msa0268690-mRNA-1:cds;Parent=Msa0268690-mRNA-1
Gene Sequence

>Msa0268690

ATGTATTCCAAATTACAAGAAAAAAACGAAGGAGGATTTGAAATGCGAGATCTAAAAACATATCTCTCTGTGGCACCAGTGGCAAGTACTCTATGGTTCGCGGTTCTAGCAGGTCTCTTGATAGAAATCAATCATATACGAATAACTGTTATTTTGGAATGTAGCAGTTGTGATAAAAAGAGTGTTAATAAGGAATCTAGGGGTATTTCTAGATATATTACTCAAAAGAATCGACACAATACACCTAGTCGATTGGAATTGAGAAAATTCTGTCCCTTTTGTTGCAAACATACGATTCACGTCGAGATAAAGAAATAG

Protein sequence

>Msa0268690

MYSKLQEKNEGGFEMRDLKTYLSVAPVASTLWFAVLAGLLIEINHIRITVILECSSCDKKSVNKESRGISRYITQKNRHNTPSRLELRKFCPFCCKHTIHVEIKK*