AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0603310

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0603310 MtrunA17_Chr4g0031521 98.310 710 6 2 1 710 1 704 0.0 1432
Msa0603310 MtrunA17_Chr4g0054811 39.141 838 316 22 1 708 1 774 2.98e-165 494
Msa0603310 MtrunA17_Chr7g0238501 57.049 305 92 3 406 710 290 555 7.74e-111 346
Msa0603310 MtrunA17_Chr7g0238501 31.593 364 183 9 1 345 1 317 1.01e-38 151
Msa0603310 MtrunA17_Chr1g0193691 51.786 336 139 2 396 708 555 890 1.06e-109 353
Msa0603310 MtrunA17_Chr1g0193691 35.081 496 225 18 1 442 1 453 2.31e-60 218
Msa0603310 MtrunA17_Chr4g0039061 58.140 301 88 3 410 710 229 491 4.13e-109 340
Msa0603310 MtrunA17_Chr4g0039061 67.188 64 21 0 258 321 173 236 5.66e-23 103
Msa0603310 MtrunA17_Chr4g0067301 41.176 272 132 1 430 701 244 487 1.77e-62 217
Msa0603310 MtrunA17_Chr4g0067301 49.254 67 34 0 255 321 132 198 3.07e-12 69.7
Msa0603310 MtrunA17_Chr2g0295991 39.706 272 134 2 430 701 235 476 5.61e-60 209
Msa0603310 MtrunA17_Chr2g0295991 47.945 73 38 0 258 330 137 209 9.79e-14 74.3
Msa0603310 MtrunA17_Chr8g0371321 31.429 280 150 5 431 705 115 357 4.54e-36 140
Msa0603310 MtrunA17_Chr1g0213351 30.877 285 155 5 423 702 108 355 7.84e-36 139
Msa0603310 MtrunA17_Chr1g0181901 30.363 303 148 6 433 705 114 383 8.40e-33 131
Msa0603310 MtrunA17_Chr3g0089691 32.667 300 141 12 418 702 8 261 2.00e-31 124
Msa0603310 MtrunA17_Chr7g0263281 30.466 279 152 6 432 705 108 349 6.81e-31 124
Msa0603310 MtrunA17_Chr3g0089711 32.192 292 140 11 426 702 99 347 1.27e-30 124
Msa0603310 MtrunA17_Chr3g0089721 32.192 292 140 11 426 702 99 347 1.58e-30 124
Msa0603310 MtrunA17_Chr7g0250061 27.857 280 160 5 431 705 112 354 2.70e-30 123
Msa0603310 MtrunA17_Chr1g0211251 28.571 280 158 5 431 705 117 359 1.21e-28 119
Msa0603310 MtrunA17_Chr7g0223641 30.588 255 108 6 455 701 3 196 1.69e-28 113
Msa0603310 MtrunA17_Chr2g0294291 27.273 275 158 5 432 701 113 350 5.04e-28 116
Msa0603310 MtrunA17_Chr1g0207571 30.325 277 151 7 432 702 98 338 1.61e-27 115
Msa0603310 MtrunA17_Chr1g0175381 26.855 283 162 8 434 710 118 361 1.54e-25 109
Msa0603310 MtrunA17_Chr7g0256751 31.488 289 140 10 426 699 100 345 3.83e-25 108
Msa0603310 MtrunA17_Chr7g0256771 30.705 241 114 8 415 645 95 292 1.05e-19 90.9
Msa0603310 MtrunA17_Chr4g0063051 27.165 254 123 8 455 706 120 313 3.63e-17 83.6
Msa0603310 MtrunA17_Chr7g0256761 28.571 182 108 6 531 706 20 185 3.88e-15 74.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0603310 AT1G07630.1 68.611 720 158 16 1 710 1 662 0.0 930
Msa0603310 AT2G28890.1 68.619 717 155 15 1 710 1 654 0.0 924
Msa0603310 AT5G02400.1 49.930 713 277 19 19 710 21 674 0.0 611
Msa0603310 AT3G09400.1 48.352 728 280 18 1 710 1 650 0.0 564
Msa0603310 AT3G09400.2 45.954 692 276 17 1 673 1 613 2.82e-166 493
Msa0603310 AT2G46920.3 47.970 394 158 9 354 708 457 842 1.40e-107 347
Msa0603310 AT2G46920.3 37.868 441 173 14 1 373 1 408 1.43e-68 241
Msa0603310 AT2G46920.1 47.970 394 158 9 354 708 457 842 1.40e-107 347
Msa0603310 AT2G46920.1 37.868 441 173 14 1 373 1 408 1.43e-68 241
Msa0603310 AT2G46920.2 47.970 394 158 9 354 708 457 842 1.40e-107 347
Msa0603310 AT2G46920.2 37.868 441 173 14 1 373 1 408 1.43e-68 241
Msa0603310 AT2G46920.4 47.970 394 158 9 354 708 457 842 1.40e-107 347
Msa0603310 AT2G46920.4 37.868 441 173 14 1 373 1 408 1.43e-68 241
Msa0603310 AT2G35350.1 53.292 319 119 7 407 708 473 778 6.21e-102 330
Msa0603310 AT2G35350.1 34.115 384 144 15 1 328 1 331 1.17e-39 157
Msa0603310 AT3G16560.1 38.141 312 155 3 390 701 207 480 5.03e-65 224
Msa0603310 AT3G16560.1 47.297 74 39 0 245 318 133 206 1.81e-12 70.9
Msa0603310 AT3G16560.4 38.141 312 155 3 390 701 207 480 5.03e-65 224
Msa0603310 AT3G16560.4 47.297 74 39 0 245 318 133 206 1.81e-12 70.9
Msa0603310 AT3G16560.2 38.141 312 155 3 390 701 207 480 5.03e-65 224
Msa0603310 AT3G16560.2 47.297 74 39 0 245 318 133 206 1.81e-12 70.9
Msa0603310 AT3G16560.3 36.268 284 143 3 390 673 207 452 4.59e-52 187
Msa0603310 AT3G16560.3 47.297 74 39 0 245 318 133 206 1.74e-12 70.9
Msa0603310 AT3G55050.1 31.183 279 146 6 432 703 121 360 1.51e-34 136
Msa0603310 AT3G55050.2 31.183 279 146 6 432 703 121 360 1.51e-34 136
Msa0603310 AT3G12620.2 29.861 288 160 5 423 705 111 361 2.62e-34 135
Msa0603310 AT3G12620.1 29.861 288 160 5 423 705 111 361 2.62e-34 135
Msa0603310 AT3G51370.2 30.421 309 162 7 404 702 4 269 5.80e-34 132
Msa0603310 AT3G17090.1 30.182 275 149 4 432 701 122 358 1.84e-33 133
Msa0603310 AT4G38520.2 31.136 273 146 5 432 699 118 353 3.54e-33 132
Msa0603310 AT4G38520.1 31.136 273 146 5 432 699 118 353 3.54e-33 132
Msa0603310 AT3G51370.1 30.325 277 151 4 431 702 115 354 1.22e-32 130
Msa0603310 AT3G51370.3 30.325 277 151 4 431 702 115 354 1.22e-32 130
Msa0603310 AT5G66080.2 31.058 293 157 6 415 702 1 253 2.19e-32 127
Msa0603310 AT5G66080.1 31.408 277 148 5 431 702 118 357 9.00e-32 128
Msa0603310 AT5G02760.1 31.636 275 144 5 432 701 109 344 4.60e-30 122
Msa0603310 AT4G33920.2 29.603 277 156 5 431 702 14 256 7.84e-30 120
Msa0603310 AT4G33920.1 29.603 277 156 5 431 702 97 339 2.12e-29 121
Msa0603310 AT5G06750.2 28.674 279 160 5 432 705 37 281 9.96e-29 117
Msa0603310 AT5G06750.3 28.674 279 160 5 432 705 119 363 1.98e-28 118
Msa0603310 AT5G06750.1 28.674 279 160 5 432 705 119 363 1.98e-28 118
Msa0603310 AT4G33920.3 31.076 251 134 5 457 702 10 226 5.67e-27 111
Msa0603310 AT3G17090.2 30.594 219 113 3 432 645 122 306 1.74e-24 105

Find 196 sgRNAs with CRISPR-Local

Find 263 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAATCCGGAACTTGCTTTAA+TGG 0.204565 4_2:-47751647 Msa0603310:CDS
ACGGTTACTGTTGTTGTTTC+CGG 0.205809 4_2:+47752974 None:intergenic
TTCCATCATTGTTATTTCTT+TGG 0.250349 4_2:-47750185 Msa0603310:CDS
CGGGAAAATTAGACAGGATT+TGG 0.261202 4_2:-47751518 Msa0603310:CDS
CAAGTGGAACAATGCACTTC+TGG 0.269345 4_2:-47750632 Msa0603310:CDS
TAAAGGATCCGTTATTAATA+TGG 0.281615 4_2:+47752938 None:intergenic
TTTGTTATGGGAAACAAAAT+AGG 0.293503 4_2:-47753017 Msa0603310:exon
TGTTAATCATTCAGATGTTT+TGG 0.294451 4_2:-47751740 Msa0603310:CDS
TTTGTTCATAAGGAGCTTAA+AGG 0.334724 4_2:-47752084 Msa0603310:CDS
AGCTTCTCTCATAGTGGATT+AGG 0.335621 4_2:-47752489 Msa0603310:CDS
ATATGGATATGTCGTGTTTA+CGG 0.335656 4_2:+47752955 None:intergenic
GCCGTTGTCGACGGCGTTCA+TGG 0.337832 4_2:-47752781 Msa0603310:CDS
AAAGGGTTGCTTTGGGATGA+TGG 0.341522 4_2:-47752066 Msa0603310:CDS
TTTGAGTAGTGAAGGTAGTT+TGG 0.342225 4_2:-47752277 Msa0603310:CDS
AGGAGCTTAAAGGGTTGCTT+TGG 0.344311 4_2:-47752074 Msa0603310:CDS
TATATTATGATGATGATATA+AGG 0.344834 4_2:+47750115 None:intergenic
TTTCTTTGGAAGGAAGGATA+TGG 0.347821 4_2:-47750171 Msa0603310:CDS
AATCCGGAACTTGCTTTAAT+GGG 0.351962 4_2:-47751646 Msa0603310:CDS
GTTGATGATGATGTTGTTGA+TGG 0.361250 4_2:-47752003 Msa0603310:CDS
TGTTGAGCAGGGTCTCCTTC+AGG 0.362431 4_2:+47750433 None:intergenic
ATCACAGCACAAGGATCATC+TGG 0.373214 4_2:+47751260 None:intergenic
CAGAAGTGCATTGTTCCACT+TGG 0.376775 4_2:+47750633 None:intergenic
AAGGGTTGCTTTGGGATGAT+GGG 0.382657 4_2:-47752065 Msa0603310:CDS
TAGTTCTTGTTCCTAGTACT+TGG 0.392726 4_2:-47753100 Msa0603310:five_prime_UTR
ACCACCACCACCAAAGTTAC+CGG 0.398826 4_2:+47752662 None:intergenic
GAATTTGAAACCGCGTGATT+CGG 0.400287 4_2:-47752025 Msa0603310:CDS
GAAAATAAACCACGATCAAT+CGG 0.401422 4_2:+47752564 None:intergenic
CTCAAGGTTACTCGAGCATT+TGG 0.402324 4_2:-47751215 Msa0603310:CDS
CCCGATCCAATAATCAGGTT+CGG 0.403582 4_2:+47751537 None:intergenic
TTGTTCATAAGGAGCTTAAA+GGG 0.406793 4_2:-47752083 Msa0603310:CDS
CCACCGGAGTGGATTCTTGC+CGG 0.406897 4_2:-47752339 Msa0603310:CDS
TCGAAATTCCACAAGGTGAT+AGG 0.408336 4_2:-47750228 Msa0603310:CDS
GAAGATAAACGGGTCGGGTC+GGG 0.411687 4_2:+47752879 None:intergenic
TATAATAATAAACAAAATCC+AGG 0.412450 4_2:+47749855 None:intergenic
AGGAGACCCTGCTCAACATT+TGG 0.412890 4_2:-47750428 Msa0603310:CDS
GAACTTTGGAAGAAGATAAA+CGG 0.420518 4_2:+47752868 None:intergenic
GAAGACTCTGTCACGTGGAC+AGG 0.422051 4_2:-47752403 Msa0603310:CDS
AAAGGAGATGGAAGTGTGAA+TGG 0.425538 4_2:-47751834 Msa0603310:CDS
AATCTGTGGTGTTTAAGATA+TGG 0.429109 4_2:+47750559 None:intergenic
GAACCCATTAAAGCAAGTTC+CGG 0.431709 4_2:+47751643 None:intergenic
TCCGGTAACTTTGGTGGTGG+TGG 0.431807 4_2:-47752663 Msa0603310:CDS
GATGGAAGTGTGAATGGGAT+AGG 0.433001 4_2:-47751828 Msa0603310:CDS
AAGCTCTTTCGCGAGCTTTG+AGG 0.433880 4_2:-47751717 Msa0603310:CDS
GGAGCTTAAAGGGTTGCTTT+GGG 0.434258 4_2:-47752073 Msa0603310:CDS
AACAAATAATCAGGTGCATC+AGG 0.434263 4_2:+47752123 None:intergenic
ATTCTCCTCGTGCTTCTTCG+CGG 0.439380 4_2:+47751858 None:intergenic
CGGAGGCGCCGGAGATTGTT+CGG 0.441737 4_2:+47752823 None:intergenic
GACATGAACCCACGCTCAAT+CGG 0.443578 4_2:+47752591 None:intergenic
CAATGGGCTCAAGGGAAAGC+AGG 0.451112 4_2:-47752222 Msa0603310:CDS
CAATGCATGATCTTGAATCA+TGG 0.452610 4_2:-47751477 Msa0603310:CDS
TTCCAAAGAAATAACAATGA+TGG 0.456946 4_2:+47750183 None:intergenic
AGATTTGACAACAAATAATC+AGG 0.457442 4_2:+47752114 None:intergenic
AGAGCTTGATAGAAGATTGA+AGG 0.459767 4_2:-47751797 Msa0603310:CDS
AAGATTTACCTCTTCAACAT+TGG 0.460239 4_2:+47751387 None:intergenic
GGTAACTTTGGTGGTGGTGG+TGG 0.463645 4_2:-47752660 Msa0603310:CDS
TGGTGCCGGATTTCTCAAAC+AGG 0.467257 4_2:-47751195 Msa0603310:intron
TATCTTAAACACCACAGATT+AGG 0.469227 4_2:-47750556 Msa0603310:CDS
AAACTGATCAACGCCGGTGC+CGG 0.470846 4_2:+47752518 None:intergenic
AACTTTGGAAGAAGATAAAC+GGG 0.472058 4_2:+47752869 None:intergenic
TGTTTCTGAGGAACATGGTT+GGG 0.473614 4_2:-47752178 Msa0603310:CDS
TAAGATGGTGATGGAGAATC+CGG 0.475384 4_2:-47751662 Msa0603310:CDS
TTTGGAAGATGATGATTCGT+TGG 0.476363 4_2:-47752259 Msa0603310:CDS
TATGGTTGGCAAGTAATGTA+TGG 0.477336 4_2:+47750577 None:intergenic
ATAACGGATCCTTTAGATGA+AGG 0.479407 4_2:-47752930 Msa0603310:CDS
TTCTTGTAATGTTGATGAAA+GGG 0.479413 4_2:-47751615 Msa0603310:CDS
TTATGATGATGATATAAGGT+TGG 0.479889 4_2:+47750119 None:intergenic
TCTTCGACCAAATGTTGAGC+AGG 0.483562 4_2:+47750421 None:intergenic
TGACCAAGTCCTTCATCTAA+AGG 0.485372 4_2:+47752921 None:intergenic
ATCATTGTTATTTCTTTGGA+AGG 0.487899 4_2:-47750181 Msa0603310:CDS
AAACAAAATAGGAAAATTAA+CGG 0.488093 4_2:-47753006 Msa0603310:CDS
CAGAGAAGCTTCTCTCATAG+TGG 0.489328 4_2:-47752495 Msa0603310:CDS
TGTGATCAATGATCGTGTTA+AGG 0.489795 4_2:-47751243 Msa0603310:CDS
TTGTTATTTCTTTGGAAGGA+AGG 0.491846 4_2:-47750177 Msa0603310:CDS
AACATTGGTACTATGATCCT+TGG 0.492918 4_2:+47751402 None:intergenic
TTCATGGATTTATACTCCTA+CGG 0.494403 4_2:-47752765 Msa0603310:CDS
TTGGATCGGGAAAATTAGAC+AGG 0.495460 4_2:-47751524 Msa0603310:CDS
TTGGGATGATGGGGTTTCAT+CGG 0.500864 4_2:-47752055 Msa0603310:CDS
AGAAGATAAACGGGTCGGGT+CGG 0.501483 4_2:+47752878 None:intergenic
AAATTAACGGTATGTTTCAC+CGG 0.503917 4_2:-47752993 Msa0603310:CDS
ACGAACCTGTTTGAGAAATC+CGG 0.503935 4_2:+47751190 None:intergenic
CACTCCGGTGGCTCCTTCAC+CGG 0.505082 4_2:+47752351 None:intergenic
ATTGAGCGTGGGTTCATGTC+CGG 0.505314 4_2:-47752588 Msa0603310:CDS
AGGGTTGCTTTGGGATGATG+GGG 0.507169 4_2:-47752064 Msa0603310:CDS
CGTTGAAGTGTTGGCGCTGA+CGG 0.507965 4_2:+47752803 None:intergenic
GCCGAACCTGATTATTGGAT+CGG 0.509482 4_2:-47751538 Msa0603310:CDS
CCGAACCTGATTATTGGATC+GGG 0.510246 4_2:-47751537 Msa0603310:CDS
AGTTTGAATTTGAGTAGTGA+AGG 0.511209 4_2:-47752285 Msa0603310:CDS
ACAACTTTCCGAACAATCTC+CGG 0.511363 4_2:-47752831 Msa0603310:CDS
CGCTCTAACGGACCGGAACT+CGG 0.513564 4_2:+47752630 None:intergenic
TCCGTTGCAACCGATTCCGA+AGG 0.513883 4_2:-47752691 Msa0603310:CDS
TGATGGTGTTGTTGTGAAAG+AGG 0.514346 4_2:-47751986 Msa0603310:CDS
TTAGAGCGTGGGTTTATGTC+CGG 0.514791 4_2:-47752615 Msa0603310:CDS
CGACATATCCATATTAATAA+CGG 0.515144 4_2:-47752946 Msa0603310:CDS
AAGGAGATGGAAGTGTGAAT+GGG 0.515548 4_2:-47751833 Msa0603310:CDS
GTTCCGGTCCGTTAGAGCGT+GGG 0.516393 4_2:-47752626 Msa0603310:CDS
GTGATCAATGATCGTGTTAA+GGG 0.516468 4_2:-47751242 Msa0603310:CDS
TCGTTTATATTTAAATTAGG+AGG 0.518447 4_2:-47749925 Msa0603310:three_prime_UTR
GACATAAACCCACGCTCTAA+CGG 0.522963 4_2:+47752618 None:intergenic
TTGGAAGATGATGATTCGTT+GGG 0.524167 4_2:-47752258 Msa0603310:CDS
ATGGAAGTGTGAATGGGATA+GGG 0.524574 4_2:-47751827 Msa0603310:CDS
GGTTTCTTCAGAGTGAACTT+TGG 0.525914 4_2:+47752854 None:intergenic
TGGGAAGTCAGAATCTTCAA+TGG 0.526449 4_2:-47752239 Msa0603310:CDS
ATGAATAGAAACCAAGTACT+AGG 0.526919 4_2:+47753089 None:intergenic
AGATGTTTATGTGATGAATG+TGG 0.528272 4_2:-47751590 Msa0603310:CDS
AGAAGCACGAGGAGAATCAA+AGG 0.529931 4_2:-47751852 Msa0603310:CDS
TTTCGCGCTGCAAAGAAAGC+TGG 0.530460 4_2:-47750391 Msa0603310:intron
TGAAGTGTTGGCGCTGACGG+AGG 0.531029 4_2:+47752806 None:intergenic
GATGTTTATGTGATGAATGT+GGG 0.533284 4_2:-47751589 Msa0603310:CDS
ATTCTCATTGTGCTTCTCAC+CGG 0.535314 4_2:+47752320 None:intergenic
CAATGTGTTGATCAAGAGAA+AGG 0.538305 4_2:-47751940 Msa0603310:CDS
ATGTCCGGTCCGATTGAGCG+TGG 0.539933 4_2:-47752600 Msa0603310:CDS
AGAAGTGCATTGTTCCACTT+GGG 0.541493 4_2:+47750634 None:intergenic
GTTGGCGCTGACGGAGGCGC+CGG 0.543294 4_2:+47752812 None:intergenic
CGACAACGGCGTTGAAGTGT+TGG 0.543904 4_2:+47752794 None:intergenic
CACTTCAACGCCGTTGTCGA+CGG 0.543934 4_2:-47752790 Msa0603310:CDS
TCTTGATGTTGCGGATAAGA+TGG 0.547525 4_2:-47751677 Msa0603310:CDS
GGGAAGTCAGAATCTTCAAT+GGG 0.550933 4_2:-47752238 Msa0603310:CDS
GCCTTCGGAATCGGTTGCAA+CGG 0.551003 4_2:+47752690 None:intergenic
TTCACCGGTACACCTTTGAT+CGG 0.551290 4_2:+47752366 None:intergenic
GTTACTCGAGCATTTGGTGC+CGG 0.551671 4_2:-47751209 Msa0603310:CDS
GATCAAAGGTGTACCGGTGA+AGG 0.551705 4_2:-47752364 Msa0603310:CDS
TCTATTGTTACTCCGATCAA+AGG 0.552274 4_2:-47752378 Msa0603310:CDS
AGTTCCGGTCCGTTAGAGCG+TGG 0.554284 4_2:-47752627 Msa0603310:CDS
CAGAATCTTCAATGGGCTCA+AGG 0.556444 4_2:-47752231 Msa0603310:CDS
TTCATCACATTACAACCTGA+AGG 0.557463 4_2:-47750448 Msa0603310:CDS
GAGAGAGATTGTGAAGTGAA+TGG 0.558486 4_2:+47753223 None:intergenic
TGGAAGAAGATAAACGGGTC+GGG 0.560735 4_2:+47752874 None:intergenic
TACTCCGATCAAAGGTGTAC+CGG 0.561660 4_2:-47752370 Msa0603310:CDS
GTTCTTGTAATGTTGATGAA+AGG 0.566141 4_2:-47751616 Msa0603310:CDS
GATCCTTTAGATGAAGGACT+TGG 0.568383 4_2:-47752924 Msa0603310:CDS
GATAAAGATAGCTCCGGCAC+CGG 0.569242 4_2:-47752531 Msa0603310:CDS
TGTCCGGTCCGATTGAGCGT+GGG 0.569809 4_2:-47752599 Msa0603310:CDS
GGAGATTGTTCGGAAAGTTG+TGG 0.572383 4_2:+47752833 None:intergenic
GTTATTGTTTGCAGCCCAAG+TGG 0.572427 4_2:-47750648 Msa0603310:intron
AATGGGCTCAAGGGAAAGCA+GGG 0.572972 4_2:-47752221 Msa0603310:CDS
TCCATGAACGCCGTCGACAA+CGG 0.574282 4_2:+47752780 None:intergenic
AAATTAGACAGGATTTGGAG+AGG 0.574734 4_2:-47751513 Msa0603310:CDS
AAACCCACGCTCTAACGGAC+CGG 0.575313 4_2:+47752623 None:intergenic
TGTTCATGTTGTTGTTTCTG+AGG 0.576807 4_2:-47752190 Msa0603310:CDS
AATGCATGATCTTGAATCAT+GGG 0.577709 4_2:-47751476 Msa0603310:CDS
AGAATCTTCAATGGGCTCAA+GGG 0.581264 4_2:-47752230 Msa0603310:CDS
GTTGTTGTTTCTGAGGAACA+TGG 0.582318 4_2:-47752183 Msa0603310:CDS
CCGGAGCTATCTTTATCCAT+CGG 0.582787 4_2:+47752537 None:intergenic
GAACCCACGCTCAATCGGAC+CGG 0.583850 4_2:+47752596 None:intergenic
TTTCGGTTCGACCAAGATCG+AGG 0.584381 4_2:-47752464 Msa0603310:CDS
TTGTTTCTGAGGAACATGGT+TGG 0.588748 4_2:-47752179 Msa0603310:CDS
GGAGTAATTATGTAAATCAG+TGG 0.593753 4_2:-47749904 Msa0603310:three_prime_UTR
ACGAGGAGAATCAAAGGAGA+TGG 0.596338 4_2:-47751846 Msa0603310:CDS
AGCTGAAAAGCACTTAAACT+AGG 0.596987 4_2:+47751427 None:intergenic
ATGTAAATCAGTGGATAGTG+AGG 0.597119 4_2:-47749895 Msa0603310:three_prime_UTR
TCATAGTACCAATGTTGAAG+AGG 0.597252 4_2:-47751395 Msa0603310:intron
TGTACCGGTGAAGGAGCCAC+CGG 0.597685 4_2:-47752355 Msa0603310:CDS
ATGTCCGGTCCGATTGATCG+TGG 0.599187 4_2:-47752573 Msa0603310:CDS
AACATCAAGATAAGACTCCT+CGG 0.601800 4_2:+47751690 None:intergenic
CGATCATTGATCACAGCACA+AGG 0.602847 4_2:+47751251 None:intergenic
ATGTTTATGTGATGAATGTG+GGG 0.604240 4_2:-47751588 Msa0603310:CDS
TCTTGTAATGTTGATGAAAG+GGG 0.604649 4_2:-47751614 Msa0603310:CDS
AGATTGAAGGAGCAATTAAG+TGG 0.604819 4_2:-47751784 Msa0603310:CDS
TTGGAAGAAGATAAACGGGT+CGG 0.606093 4_2:+47752873 None:intergenic
CTTCGACCAAATGTTGAGCA+GGG 0.606676 4_2:+47750422 None:intergenic
ATAATAATAAACAAAATCCA+GGG 0.607632 4_2:+47749856 None:intergenic
GATTGTAAAGTTGTAAACCC+TGG 0.609142 4_2:-47749873 Msa0603310:three_prime_UTR
TCAACACTCGTGAATAGCGA+GGG 0.611782 4_2:+47750030 None:intergenic
AAGACTCTGTCACGTGGACA+GGG 0.611869 4_2:-47752402 Msa0603310:CDS
GCTATCTTTATCCATCGGAC+CGG 0.612481 4_2:+47752542 None:intergenic
TCGTGTTAAGGGATCTCTCA+AGG 0.616145 4_2:-47751231 Msa0603310:CDS
CCGGCAAGAATCCACTCCGG+TGG 0.621535 4_2:+47752339 None:intergenic
TGTGGTGTTTAAGATATGGT+TGG 0.627286 4_2:+47750563 None:intergenic
GGAGTCTTATCTTGATGTTG+CGG 0.628468 4_2:-47751686 Msa0603310:CDS
ATGAGATATATGCTGCCGAA+GGG 0.630709 4_2:-47750077 Msa0603310:three_prime_UTR
TATGAGATATATGCTGCCGA+AGG 0.630877 4_2:-47750078 Msa0603310:three_prime_UTR
TAACGCCGCCTATCACCTTG+TGG 0.632464 4_2:+47750220 None:intergenic
CAAGTATTGATAGAGTCCGT+CGG 0.633991 4_2:+47750504 None:intergenic
TCACAGCACAAGGATCATCT+GGG 0.640014 4_2:+47751261 None:intergenic
CTCAACACTCGTGAATAGCG+AGG 0.640521 4_2:+47750029 None:intergenic
CCGATGGATAAAGATAGCTC+CGG 0.640588 4_2:-47752537 Msa0603310:CDS
TAAACCACGATCAATCGGAC+CGG 0.640729 4_2:+47752569 None:intergenic
TGTTGCGGATAAGATGGTGA+TGG 0.644164 4_2:-47751671 Msa0603310:CDS
ATGATTAACAGAATTATCGT+CGG 0.649688 4_2:+47751753 None:intergenic
CGGTGAAGGAGCCACCGGAG+TGG 0.652691 4_2:-47752350 Msa0603310:CDS
GCGAGCTTTGAGGAAAACCG+AGG 0.665283 4_2:-47751707 Msa0603310:CDS
AGATGCCGCGAAGAAGCACG+AGG 0.671726 4_2:-47751863 Msa0603310:CDS
AAATTCCACAAGGTGATAGG+CGG 0.673197 4_2:-47750225 Msa0603310:CDS
AGCTCTATCAAAACAACCGT+AGG 0.682369 4_2:+47752749 None:intergenic
TCACCGGCAAGAATCCACTC+CGG 0.682589 4_2:+47752336 None:intergenic
ATTTCGAAGACTCTGTCACG+TGG 0.685701 4_2:-47752408 Msa0603310:CDS
TTGAAGGAGCAATTAAGTGG+CGG 0.690975 4_2:-47751781 Msa0603310:CDS
TCTCTGAAACTGATCAACGC+CGG 0.696982 4_2:+47752512 None:intergenic
CAACACTCGTGAATAGCGAG+GGG 0.699953 4_2:+47750031 None:intergenic
ATGTTTAGAATAGACTACGT+AGG 0.701650 4_2:-47750607 Msa0603310:CDS
CAATGATGGAAACATCGTCG+TGG 0.706639 4_2:+47750197 None:intergenic
ATGGGCTCAAGGGAAAGCAG+GGG 0.712223 4_2:-47752220 Msa0603310:CDS
TCATCATCAACCGAATCACG+CGG 0.743225 4_2:+47752015 None:intergenic
TGTTTATGTGATGAATGTGG+GGG 0.749920 4_2:-47751587 Msa0603310:CDS
GAATTGCTCGAAATTCCACA+AGG 0.753407 4_2:-47750235 Msa0603310:CDS
AACACTCGTGAATAGCGAGG+GGG 0.759764 4_2:+47750032 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGTTTTTTAATTTTTGTTAT+GGG - chr4_2:47749985-47750004 Msa0603310:three_prime_UTR 10.0%
!!! TTTTCGTTTATATTTAAATT+AGG - chr4_2:47753086-47753105 Msa0603310:five_prime_UTR 10.0%
!!! GTGTTTTTTAATTTTTGTTA+TGG - chr4_2:47749984-47750003 Msa0603310:three_prime_UTR 15.0%
!! AAACAAAATAGGAAAATTAA+CGG - chr4_2:47750008-47750027 Msa0603310:three_prime_UTR 15.0%
!!! TATATTATGATGATGATATA+AGG + chr4_2:47752902-47752921 None:intergenic 15.0%
!!! TTTTAGCTTATATTTAAGTT+CGG + chr4_2:47753024-47753043 None:intergenic 15.0%
!! ATAATAATAAACAAAATCCA+GGG + chr4_2:47753161-47753180 None:intergenic 15.0%
!! TATAATAATAAACAAAATCC+AGG + chr4_2:47753162-47753181 None:intergenic 15.0%
!!! TTTTGTGTTGAAAAATTAGT+GGG + chr4_2:47749836-47749855 None:intergenic 20.0%
!!! TCTTTATAGTTTTGTTCATA+AGG - chr4_2:47750920-47750939 Msa0603310:intron 20.0%
!! ATTTCAAAGAAAAAAAAGAG+AGG - chr4_2:47751104-47751123 Msa0603310:intron 20.0%
!! AAGTGTTTGATTATAATTTC+AGG - chr4_2:47751705-47751724 Msa0603310:CDS 20.0%
!! TTATTAATCTTCTACAAGAA+TGG + chr4_2:47752102-47752121 None:intergenic 20.0%
!!! GAATCAAAAATTTGTCTTTT+TGG + chr4_2:47752484-47752503 None:intergenic 20.0%
!! TATTCTTCTTAATCTTAATC+AGG - chr4_2:47752743-47752762 Msa0603310:CDS 20.0%
!! TCGTTTATATTTAAATTAGG+AGG - chr4_2:47753089-47753108 Msa0603310:five_prime_UTR 20.0%
!!! ATGAAAAGATTTTTAAGGAA+TGG + chr4_2:47753223-47753242 None:intergenic 20.0%
!! GTTTTGTGTTGAAAAATTAG+TGG + chr4_2:47749837-47749856 None:intergenic 25.0%
!!! ATAACTCGTTTTTCATGTTT+TGG + chr4_2:47749859-47749878 None:intergenic 25.0%
!! GAACTATGAAACAAGTTTTA+AGG + chr4_2:47749900-47749919 None:intergenic 25.0%
!! TTTGTTATGGGAAACAAAAT+AGG - chr4_2:47749997-47750016 Msa0603310:three_prime_UTR 25.0%
! CGACATATCCATATTAATAA+CGG - chr4_2:47750068-47750087 Msa0603310:three_prime_UTR 25.0%
! TAAAGGATCCGTTATTAATA+TGG + chr4_2:47750079-47750098 None:intergenic 25.0%
! AGATTTGACAACAAATAATC+AGG + chr4_2:47750903-47750922 None:intergenic 25.0%
! ATGATTAACAGAATTATCGT+CGG + chr4_2:47751264-47751283 None:intergenic 25.0%
!! TGTTAATCATTCAGATGTTT+TGG - chr4_2:47751274-47751293 Msa0603310:CDS 25.0%
!! TTCTTGTAATGTTGATGAAA+GGG - chr4_2:47751399-47751418 Msa0603310:CDS 25.0%
! CCATTAATTAAGAAACTCAA+CGG - chr4_2:47751887-47751906 Msa0603310:CDS 25.0%
!! AATCATATATCCTATCCTTT+TGG + chr4_2:47752006-47752025 None:intergenic 25.0%
! TTCTTTCCTTTCACAAAAAA+TGG - chr4_2:47752145-47752164 Msa0603310:CDS 25.0%
!!! AATGTTCCATTTTTTGTGAA+AGG + chr4_2:47752154-47752173 None:intergenic 25.0%
! TGAAAATCCATTAAAGAGTT+AGG + chr4_2:47752181-47752200 None:intergenic 25.0%
! AACAGAAAATGTTTCATACT+TGG - chr4_2:47752275-47752294 Msa0603310:CDS 25.0%
! TAACATTTGTAGTTAGAGTT+AGG + chr4_2:47752350-47752369 None:intergenic 25.0%
! GCAATTGTGAAATCTTAAAA+TGG + chr4_2:47752660-47752679 None:intergenic 25.0%
!!! TTCCATCATTGTTATTTCTT+TGG - chr4_2:47752829-47752848 Msa0603310:CDS 25.0%
! TTCCAAAGAAATAACAATGA+TGG + chr4_2:47752834-47752853 None:intergenic 25.0%
!!! ATCATTGTTATTTCTTTGGA+AGG - chr4_2:47752833-47752852 Msa0603310:CDS 25.0%
!! TTATGATGATGATATAAGGT+TGG + chr4_2:47752898-47752917 None:intergenic 25.0%
ATGAATAGAAACCAAGTACT+AGG + chr4_2:47749928-47749947 None:intergenic 30.0%
AAATTAACGGTATGTTTCAC+CGG - chr4_2:47750021-47750040 Msa0603310:three_prime_UTR 30.0%
ATATGGATATGTCGTGTTTA+CGG + chr4_2:47750062-47750081 None:intergenic 30.0%
! AACTTTGGAAGAAGATAAAC+GGG + chr4_2:47750148-47750167 None:intergenic 30.0%
! GAACTTTGGAAGAAGATAAA+CGG + chr4_2:47750149-47750168 None:intergenic 30.0%
TTCATGGATTTATACTCCTA+CGG - chr4_2:47750249-47750268 Msa0603310:CDS 30.0%
GAAAATAAACCACGATCAAT+CGG + chr4_2:47750453-47750472 None:intergenic 30.0%
!! ATTGATCGTGGTTTATTTTC+CGG - chr4_2:47750453-47750472 Msa0603310:CDS 30.0%
!!! TCATAGTGGATTAGGTTTTT+CGG - chr4_2:47750533-47750552 Msa0603310:CDS 30.0%
AGTTTGAATTTGAGTAGTGA+AGG - chr4_2:47750729-47750748 Msa0603310:intron 30.0%
! GTTTTTGTTGGGATTTATGA+TGG - chr4_2:47750858-47750877 Msa0603310:intron 30.0%
!!! GGGATTTATGATGGTTTTAA+TGG - chr4_2:47750867-47750886 Msa0603310:intron 30.0%
TTTGTTCATAAGGAGCTTAA+AGG - chr4_2:47750930-47750949 Msa0603310:intron 30.0%
TTGTTCATAAGGAGCTTAAA+GGG - chr4_2:47750931-47750950 Msa0603310:intron 30.0%
! GTTCTTGTAATGTTGATGAA+AGG - chr4_2:47751398-47751417 Msa0603310:CDS 30.0%
! TCTTGTAATGTTGATGAAAG+GGG - chr4_2:47751400-47751419 Msa0603310:CDS 30.0%
AGATGTTTATGTGATGAATG+TGG - chr4_2:47751424-47751443 Msa0603310:CDS 30.0%
GATGTTTATGTGATGAATGT+GGG - chr4_2:47751425-47751444 Msa0603310:CDS 30.0%
ATGTTTATGTGATGAATGTG+GGG - chr4_2:47751426-47751445 Msa0603310:CDS 30.0%
AATGCATGATCTTGAATCAT+GGG - chr4_2:47751538-47751557 Msa0603310:CDS 30.0%
AAGATTTACCTCTTCAACAT+TGG + chr4_2:47751630-47751649 None:intergenic 30.0%
CCGTTGAGTTTCTTAATTAA+TGG + chr4_2:47751890-47751909 None:intergenic 30.0%
TAATTAAGAAACTCAACGGA+AGG - chr4_2:47751891-47751910 Msa0603310:CDS 30.0%
! ATATCCTATCCTTTTGGATT+TGG + chr4_2:47752000-47752019 None:intergenic 30.0%
! ATTTGTTTCTCTTGTCACAA+AGG - chr4_2:47752059-47752078 Msa0603310:CDS 30.0%
ATTGACACCTAACTCTTTAA+TGG - chr4_2:47752171-47752190 Msa0603310:CDS 30.0%
TGTCATATTAATGCTAAGCA+TGG - chr4_2:47752205-47752224 Msa0603310:CDS 30.0%
! ATGTTTAGAATAGACTACGT+AGG - chr4_2:47752407-47752426 Msa0603310:CDS 30.0%
! AATCTGTGGTGTTTAAGATA+TGG + chr4_2:47752458-47752477 None:intergenic 30.0%
TATCTTAAACACCACAGATT+AGG - chr4_2:47752458-47752477 Msa0603310:CDS 30.0%
TTCTTAATCTTAATCAGGTC+TGG - chr4_2:47752748-47752767 Msa0603310:CDS 30.0%
!! TTGTTATTTCTTTGGAAGGA+AGG - chr4_2:47752837-47752856 Msa0603310:CDS 30.0%
GGAGTAATTATGTAAATCAG+TGG - chr4_2:47753110-47753129 Msa0603310:five_prime_UTR 30.0%
TAGTTCTTGTTCCTAGTACT+TGG - chr4_2:47749914-47749933 Msa0603310:three_prime_UTR 35.0%
!! ATAACGGATCCTTTAGATGA+AGG - chr4_2:47750084-47750103 Msa0603310:three_prime_UTR 35.0%
! CATTTTTCTTTCCTCGATCT+TGG + chr4_2:47750564-47750583 None:intergenic 35.0%
CAAGATCGAGGAAAGAAAAA+TGG - chr4_2:47750562-47750581 Msa0603310:CDS 35.0%
TCTATTGTTACTCCGATCAA+AGG - chr4_2:47750636-47750655 Msa0603310:CDS 35.0%
TTTGAGTAGTGAAGGTAGTT+TGG - chr4_2:47750737-47750756 Msa0603310:intron 35.0%
TTTGGAAGATGATGATTCGT+TGG - chr4_2:47750755-47750774 Msa0603310:intron 35.0%
TTGGAAGATGATGATTCGTT+GGG - chr4_2:47750756-47750775 Msa0603310:intron 35.0%
!! TGTTCATGTTGTTGTTTCTG+AGG - chr4_2:47750824-47750843 Msa0603310:intron 35.0%
! AACATGGTTGGGTTTTTGTT+GGG - chr4_2:47750847-47750866 Msa0603310:intron 35.0%
AACAAATAATCAGGTGCATC+AGG + chr4_2:47750894-47750913 None:intergenic 35.0%
!! GTTGATGATGATGTTGTTGA+TGG - chr4_2:47751011-47751030 Msa0603310:intron 35.0%
! CAATGTGTTGATCAAGAGAA+AGG - chr4_2:47751074-47751093 Msa0603310:intron 35.0%
AGAGCTTGATAGAAGATTGA+AGG - chr4_2:47751217-47751236 Msa0603310:CDS 35.0%
AGATTGAAGGAGCAATTAAG+TGG - chr4_2:47751230-47751249 Msa0603310:CDS 35.0%
AACATCAAGATAAGACTCCT+CGG + chr4_2:47751327-47751346 None:intergenic 35.0%
! AATCCGGAACTTGCTTTAAT+GGG - chr4_2:47751368-47751387 Msa0603310:intron 35.0%
TGTTTATGTGATGAATGTGG+GGG - chr4_2:47751427-47751446 Msa0603310:CDS 35.0%
AAAAAGCCGAACCTGATTAT+TGG - chr4_2:47751471-47751490 Msa0603310:CDS 35.0%
! ATTTTCCCGATCCAATAATC+AGG + chr4_2:47751485-47751504 None:intergenic 35.0%
AAATTAGACAGGATTTGGAG+AGG - chr4_2:47751501-47751520 Msa0603310:CDS 35.0%
CAATGCATGATCTTGAATCA+TGG - chr4_2:47751537-47751556 Msa0603310:CDS 35.0%
!! AGCTGAAAAGCACTTAAACT+AGG + chr4_2:47751590-47751609 None:intergenic 35.0%
AACATTGGTACTATGATCCT+TGG + chr4_2:47751615-47751634 None:intergenic 35.0%
TCATAGTACCAATGTTGAAG+AGG - chr4_2:47751619-47751638 Msa0603310:CDS 35.0%
CCAAAACAGAAGTCTAAAAG+AGG + chr4_2:47751655-47751674 None:intergenic 35.0%
! CCTCTTTTAGACTTCTGTTT+TGG - chr4_2:47751652-47751671 Msa0603310:CDS 35.0%
TGTGATCAATGATCGTGTTA+AGG - chr4_2:47751771-47751790 Msa0603310:CDS 35.0%
GTGATCAATGATCGTGTTAA+GGG - chr4_2:47751772-47751791 Msa0603310:CDS 35.0%
! CTATCCTTTTGGATTTGGAT+TGG + chr4_2:47751995-47752014 None:intergenic 35.0%
CAATCCAAATCCAAAAGGAT+AGG - chr4_2:47751993-47752012 Msa0603310:CDS 35.0%
TGTGACAAGAGAAACAAATG+TGG + chr4_2:47752058-47752077 None:intergenic 35.0%
CACAAAGGAATACATATGCT+AGG - chr4_2:47752074-47752093 Msa0603310:CDS 35.0%
TATGGTTGGCAAGTAATGTA+TGG + chr4_2:47752440-47752459 None:intergenic 35.0%
! TGTGGTGTTTAAGATATGGT+TGG + chr4_2:47752454-47752473 None:intergenic 35.0%
!! TTTTTGATTCTATGCTCCGA+CGG - chr4_2:47752494-47752513 Msa0603310:CDS 35.0%
TTCATCACATTACAACCTGA+AGG - chr4_2:47752566-47752585 Msa0603310:CDS 35.0%
! TTTCTTTGGAAGGAAGGATA+TGG - chr4_2:47752843-47752862 Msa0603310:CDS 35.0%
ATGTAAATCAGTGGATAGTG+AGG - chr4_2:47753119-47753138 Msa0603310:five_prime_UTR 35.0%
GATTGTAAAGTTGTAAACCC+TGG - chr4_2:47753141-47753160 Msa0603310:five_prime_UTR 35.0%
!! ACGGTTACTGTTGTTGTTTC+CGG + chr4_2:47750043-47750062 None:intergenic 40.0%
GATCCTTTAGATGAAGGACT+TGG - chr4_2:47750090-47750109 Msa0603310:three_prime_UTR 40.0%
TGACCAAGTCCTTCATCTAA+AGG + chr4_2:47750096-47750115 None:intergenic 40.0%
TTGGAAGAAGATAAACGGGT+CGG + chr4_2:47750144-47750163 None:intergenic 40.0%
GGTTTCTTCAGAGTGAACTT+TGG + chr4_2:47750163-47750182 None:intergenic 40.0%
ACAACTTTCCGAACAATCTC+CGG - chr4_2:47750183-47750202 Msa0603310:CDS 40.0%
AGCTCTATCAAAACAACCGT+AGG + chr4_2:47750268-47750287 None:intergenic 40.0%
AAGTTACCGGAAAAAGCCTT+CGG + chr4_2:47750342-47750361 None:intergenic 40.0%
!! AAGGCTTTTTCCGGTAACTT+TGG - chr4_2:47750342-47750361 Msa0603310:intron 40.0%
!! AGCTTCTCTCATAGTGGATT+AGG - chr4_2:47750525-47750544 Msa0603310:CDS 40.0%
ATTCTCATTGTGCTTCTCAC+CGG + chr4_2:47750697-47750716 None:intergenic 40.0%
TGGGAAGTCAGAATCTTCAA+TGG - chr4_2:47750775-47750794 Msa0603310:intron 40.0%
GGGAAGTCAGAATCTTCAAT+GGG - chr4_2:47750776-47750795 Msa0603310:intron 40.0%
! AGAATCTTCAATGGGCTCAA+GGG - chr4_2:47750784-47750803 Msa0603310:intron 40.0%
!! GTTGTTGTTTCTGAGGAACA+TGG - chr4_2:47750831-47750850 Msa0603310:intron 40.0%
!! TTGTTTCTGAGGAACATGGT+TGG - chr4_2:47750835-47750854 Msa0603310:intron 40.0%
! TGTTTCTGAGGAACATGGTT+GGG - chr4_2:47750836-47750855 Msa0603310:intron 40.0%
! GAACATGGTTGGGTTTTTGT+TGG - chr4_2:47750846-47750865 Msa0603310:intron 40.0%
! GAATTTGAAACCGCGTGATT+CGG - chr4_2:47750989-47751008 Msa0603310:intron 40.0%
!! TGATGGTGTTGTTGTGAAAG+AGG - chr4_2:47751028-47751047 Msa0603310:intron 40.0%
! AAAGGAGATGGAAGTGTGAA+TGG - chr4_2:47751180-47751199 Msa0603310:intron 40.0%
! AAGGAGATGGAAGTGTGAAT+GGG - chr4_2:47751181-47751200 Msa0603310:intron 40.0%
! ATGGAAGTGTGAATGGGATA+GGG - chr4_2:47751187-47751206 Msa0603310:intron 40.0%
TTGAAGGAGCAATTAAGTGG+CGG - chr4_2:47751233-47751252 Msa0603310:CDS 40.0%
! GGAGTCTTATCTTGATGTTG+CGG - chr4_2:47751328-47751347 Msa0603310:intron 40.0%
TCTTGATGTTGCGGATAAGA+TGG - chr4_2:47751337-47751356 Msa0603310:intron 40.0%
!! TAAGATGGTGATGGAGAATC+CGG - chr4_2:47751352-47751371 Msa0603310:intron 40.0%
! GAATCCGGAACTTGCTTTAA+TGG - chr4_2:47751367-47751386 Msa0603310:intron 40.0%
GAACCCATTAAAGCAAGTTC+CGG + chr4_2:47751374-47751393 None:intergenic 40.0%
TTGGATCGGGAAAATTAGAC+AGG - chr4_2:47751490-47751509 Msa0603310:CDS 40.0%
CGGGAAAATTAGACAGGATT+TGG - chr4_2:47751496-47751515 Msa0603310:CDS 40.0%
! AAGTGCTTTTCAGCTTACCA+AGG - chr4_2:47751595-47751614 Msa0603310:CDS 40.0%
ACGAACCTGTTTGAGAAATC+CGG + chr4_2:47751827-47751846 None:intergenic 40.0%
TAAGAAACTCAACGGAAGGA+TGG - chr4_2:47751895-47751914 Msa0603310:CDS 40.0%
! GGATGGATTTAGATTGCTTG+CGG - chr4_2:47751912-47751931 Msa0603310:CDS 40.0%
!!! TTTTGGATTTGGATTGGCTG+CGG + chr4_2:47751989-47752008 None:intergenic 40.0%
! AGAAGTGCATTGTTCCACTT+GGG + chr4_2:47752383-47752402 None:intergenic 40.0%
!! TGTCTTTTTGGCCTAATCTG+TGG + chr4_2:47752472-47752491 None:intergenic 40.0%
!! CAAGTATTGATAGAGTCCGT+CGG + chr4_2:47752513-47752532 None:intergenic 40.0%
GAATTGCTCGAAATTCCACA+AGG - chr4_2:47752779-47752798 Msa0603310:CDS 40.0%
TCGAAATTCCACAAGGTGAT+AGG - chr4_2:47752786-47752805 Msa0603310:CDS 40.0%
AAATTCCACAAGGTGATAGG+CGG - chr4_2:47752789-47752808 Msa0603310:CDS 40.0%
TATGAGATATATGCTGCCGA+AGG - chr4_2:47752936-47752955 Msa0603310:CDS 40.0%
ATGAGATATATGCTGCCGAA+GGG - chr4_2:47752937-47752956 Msa0603310:CDS 40.0%
TGGAAGAAGATAAACGGGTC+GGG + chr4_2:47750143-47750162 None:intergenic 45.0%
GGAGATTGTTCGGAAAGTTG+TGG + chr4_2:47750184-47750203 None:intergenic 45.0%
!! GCTTTTTCCGGTAACTTTGG+TGG - chr4_2:47750345-47750364 Msa0603310:intron 45.0%
! TTTTCCGGTAACTTTGGTGG+TGG - chr4_2:47750348-47750367 Msa0603310:intron 45.0%
GACATAAACCCACGCTCTAA+CGG + chr4_2:47750399-47750418 None:intergenic 45.0%
TTAGAGCGTGGGTTTATGTC+CGG - chr4_2:47750399-47750418 Msa0603310:CDS 45.0%
TAAACCACGATCAATCGGAC+CGG + chr4_2:47750448-47750467 None:intergenic 45.0%
!! TGGTTTATTTTCCGGTCCGA+TGG - chr4_2:47750461-47750480 Msa0603310:CDS 45.0%
GCTATCTTTATCCATCGGAC+CGG + chr4_2:47750475-47750494 None:intergenic 45.0%
CCGGAGCTATCTTTATCCAT+CGG + chr4_2:47750480-47750499 None:intergenic 45.0%
CCGATGGATAAAGATAGCTC+CGG - chr4_2:47750477-47750496 Msa0603310:CDS 45.0%
!! TCTCTGAAACTGATCAACGC+CGG + chr4_2:47750505-47750524 None:intergenic 45.0%
CAGAGAAGCTTCTCTCATAG+TGG - chr4_2:47750519-47750538 Msa0603310:CDS 45.0%
ATTTCGAAGACTCTGTCACG+TGG - chr4_2:47750606-47750625 Msa0603310:CDS 45.0%
! TACTCCGATCAAAGGTGTAC+CGG - chr4_2:47750644-47750663 Msa0603310:intron 45.0%
TTCACCGGTACACCTTTGAT+CGG + chr4_2:47750651-47750670 None:intergenic 45.0%
! CAGAATCTTCAATGGGCTCA+AGG - chr4_2:47750783-47750802 Msa0603310:intron 45.0%
AGGAGCTTAAAGGGTTGCTT+TGG - chr4_2:47750940-47750959 Msa0603310:intron 45.0%
! GGAGCTTAAAGGGTTGCTTT+GGG - chr4_2:47750941-47750960 Msa0603310:intron 45.0%
!! AAAGGGTTGCTTTGGGATGA+TGG - chr4_2:47750948-47750967 Msa0603310:intron 45.0%
!! AAGGGTTGCTTTGGGATGAT+GGG - chr4_2:47750949-47750968 Msa0603310:intron 45.0%
!! TTGGGATGATGGGGTTTCAT+CGG - chr4_2:47750959-47750978 Msa0603310:intron 45.0%
TCATCATCAACCGAATCACG+CGG + chr4_2:47751002-47751021 None:intergenic 45.0%
! AGAAGCACGAGGAGAATCAA+AGG - chr4_2:47751162-47751181 Msa0603310:intron 45.0%
ACGAGGAGAATCAAAGGAGA+TGG - chr4_2:47751168-47751187 Msa0603310:intron 45.0%
! GATGGAAGTGTGAATGGGAT+AGG - chr4_2:47751186-47751205 Msa0603310:intron 45.0%
TGTTGCGGATAAGATGGTGA+TGG - chr4_2:47751343-47751362 Msa0603310:intron 45.0%
GCCGAACCTGATTATTGGAT+CGG - chr4_2:47751476-47751495 Msa0603310:CDS 45.0%
CCCGATCCAATAATCAGGTT+CGG + chr4_2:47751480-47751499 None:intergenic 45.0%
CCGAACCTGATTATTGGATC+GGG - chr4_2:47751477-47751496 Msa0603310:CDS 45.0%
TCACAGCACAAGGATCATCT+GGG + chr4_2:47751756-47751775 None:intergenic 45.0%
ATCACAGCACAAGGATCATC+TGG + chr4_2:47751757-47751776 None:intergenic 45.0%
CGATCATTGATCACAGCACA+AGG + chr4_2:47751766-47751785 None:intergenic 45.0%
TCGTGTTAAGGGATCTCTCA+AGG - chr4_2:47751783-47751802 Msa0603310:CDS 45.0%
CTCAAGGTTACTCGAGCATT+TGG - chr4_2:47751799-47751818 Msa0603310:CDS 45.0%
AATCCCACACAGTTAACACG+AGG - chr4_2:47751939-47751958 Msa0603310:CDS 45.0%
AAGCCTCGTGTTAACTGTGT+GGG + chr4_2:47751945-47751964 None:intergenic 45.0%
AAAGCCTCGTGTTAACTGTG+TGG + chr4_2:47751946-47751965 None:intergenic 45.0%
GCAGCCAATCCAAATCCAAA+AGG - chr4_2:47751988-47752007 Msa0603310:CDS 45.0%
GTTATTGTTTGCAGCCCAAG+TGG - chr4_2:47752366-47752385 Msa0603310:CDS 45.0%
! CAGAAGTGCATTGTTCCACT+TGG + chr4_2:47752384-47752403 None:intergenic 45.0%
CAAGTGGAACAATGCACTTC+TGG - chr4_2:47752382-47752401 Msa0603310:CDS 45.0%
CTTCGACCAAATGTTGAGCA+GGG + chr4_2:47752595-47752614 None:intergenic 45.0%
TCTTCGACCAAATGTTGAGC+AGG + chr4_2:47752596-47752615 None:intergenic 45.0%
! CAATGATGGAAACATCGTCG+TGG + chr4_2:47752820-47752839 None:intergenic 45.0%
AAAAAGGATGCGTGACCCTT+CGG + chr4_2:47752955-47752974 None:intergenic 45.0%
TCAACACTCGTGAATAGCGA+GGG + chr4_2:47752987-47753006 None:intergenic 45.0%
AGAAGATAAACGGGTCGGGT+CGG + chr4_2:47750139-47750158 None:intergenic 50.0%
CCGGAAAAAGCCTTCGGAAT+CGG + chr4_2:47750336-47750355 None:intergenic 50.0%
!! CCGATTCCGAAGGCTTTTTC+CGG - chr4_2:47750333-47750352 Msa0603310:intron 50.0%
ACCACCACCACCAAAGTTAC+CGG + chr4_2:47750355-47750374 None:intergenic 50.0%
GACATGAACCCACGCTCAAT+CGG + chr4_2:47750426-47750445 None:intergenic 50.0%
ATTGAGCGTGGGTTCATGTC+CGG - chr4_2:47750426-47750445 Msa0603310:CDS 50.0%
GATAAAGATAGCTCCGGCAC+CGG - chr4_2:47750483-47750502 Msa0603310:CDS 50.0%
! TTTCGGTTCGACCAAGATCG+AGG - chr4_2:47750550-47750569 Msa0603310:CDS 50.0%
AAGACTCTGTCACGTGGACA+GGG - chr4_2:47750612-47750631 Msa0603310:CDS 50.0%
! GATCAAAGGTGTACCGGTGA+AGG - chr4_2:47750650-47750669 Msa0603310:intron 50.0%
! AATGGGCTCAAGGGAAAGCA+GGG - chr4_2:47750793-47750812 Msa0603310:intron 50.0%
!! AGGGTTGCTTTGGGATGATG+GGG - chr4_2:47750950-47750969 Msa0603310:intron 50.0%
ATTCTCCTCGTGCTTCTTCG+CGG + chr4_2:47751159-47751178 None:intergenic 50.0%
AAGCTCTTTCGCGAGCTTTG+AGG - chr4_2:47751297-47751316 Msa0603310:intron 50.0%
! GGGGGATAGTAGAGCTGTTT+TGG - chr4_2:47751445-47751464 Msa0603310:CDS 50.0%
!! GTTACTCGAGCATTTGGTGC+CGG - chr4_2:47751805-47751824 Msa0603310:CDS 50.0%
! TGGTGCCGGATTTCTCAAAC+AGG - chr4_2:47751819-47751838 Msa0603310:CDS 50.0%
AGGAGACCCTGCTCAACATT+TGG - chr4_2:47752586-47752605 Msa0603310:CDS 50.0%
TTTCGCGCTGCAAAGAAAGC+TGG - chr4_2:47752623-47752642 Msa0603310:CDS 50.0%
GCGAGGGGGTAAGAACAAAA+AGG + chr4_2:47752971-47752990 None:intergenic 50.0%
AACACTCGTGAATAGCGAGG+GGG + chr4_2:47752985-47753004 None:intergenic 50.0%
CAACACTCGTGAATAGCGAG+GGG + chr4_2:47752986-47753005 None:intergenic 50.0%
CTCAACACTCGTGAATAGCG+AGG + chr4_2:47752988-47753007 None:intergenic 50.0%
GAAGATAAACGGGTCGGGTC+GGG + chr4_2:47750138-47750157 None:intergenic 55.0%
!! CGTTGAAGTGTTGGCGCTGA+CGG + chr4_2:47750214-47750233 None:intergenic 55.0%
! CGACAACGGCGTTGAAGTGT+TGG + chr4_2:47750223-47750242 None:intergenic 55.0%
CACTTCAACGCCGTTGTCGA+CGG - chr4_2:47750224-47750243 Msa0603310:CDS 55.0%
TCCATGAACGCCGTCGACAA+CGG + chr4_2:47750237-47750256 None:intergenic 55.0%
! TCCGTTGCAACCGATTCCGA+AGG - chr4_2:47750323-47750342 Msa0603310:intron 55.0%
GCCTTCGGAATCGGTTGCAA+CGG + chr4_2:47750327-47750346 None:intergenic 55.0%
!! TCCGGTAACTTTGGTGGTGG+TGG - chr4_2:47750351-47750370 Msa0603310:intron 55.0%
!! GGTAACTTTGGTGGTGGTGG+TGG - chr4_2:47750354-47750373 Msa0603310:intron 55.0%
!! AACTTTGGTGGTGGTGGTGG+TGG - chr4_2:47750357-47750376 Msa0603310:intron 55.0%
!! GGTGGTGGTTTTCCGAGTTC+CGG - chr4_2:47750372-47750391 Msa0603310:intron 55.0%
! AAACCCACGCTCTAACGGAC+CGG + chr4_2:47750394-47750413 None:intergenic 55.0%
ATGTCCGGTCCGATTGATCG+TGG - chr4_2:47750441-47750460 Msa0603310:CDS 55.0%
AAACTGATCAACGCCGGTGC+CGG + chr4_2:47750499-47750518 None:intergenic 55.0%
GAAGACTCTGTCACGTGGAC+AGG - chr4_2:47750611-47750630 Msa0603310:CDS 55.0%
TCACCGGCAAGAATCCACTC+CGG + chr4_2:47750681-47750700 None:intergenic 55.0%
! CAATGGGCTCAAGGGAAAGC+AGG - chr4_2:47750792-47750811 Msa0603310:intron 55.0%
ATGGGCTCAAGGGAAAGCAG+GGG - chr4_2:47750794-47750813 Msa0603310:intron 55.0%
!! GCGAGCTTTGAGGAAAACCG+AGG - chr4_2:47751307-47751326 Msa0603310:intron 55.0%
TGTTGAGCAGGGTCTCCTTC+AGG + chr4_2:47752584-47752603 None:intergenic 55.0%
TAACGCCGCCTATCACCTTG+TGG + chr4_2:47752797-47752816 None:intergenic 55.0%
!! TGAAGTGTTGGCGCTGACGG+AGG + chr4_2:47750211-47750230 None:intergenic 60.0%
! CGCTCTAACGGACCGGAACT+CGG + chr4_2:47750387-47750406 None:intergenic 60.0%
AGTTCCGGTCCGTTAGAGCG+TGG - chr4_2:47750387-47750406 Msa0603310:intron 60.0%
GTTCCGGTCCGTTAGAGCGT+GGG - chr4_2:47750388-47750407 Msa0603310:intron 60.0%
ATGTCCGGTCCGATTGAGCG+TGG - chr4_2:47750414-47750433 Msa0603310:CDS 60.0%
TGTCCGGTCCGATTGAGCGT+GGG - chr4_2:47750415-47750434 Msa0603310:CDS 60.0%
GAACCCACGCTCAATCGGAC+CGG + chr4_2:47750421-47750440 None:intergenic 60.0%
! TGTACCGGTGAAGGAGCCAC+CGG - chr4_2:47750659-47750678 Msa0603310:intron 60.0%
! CCACCGGAGTGGATTCTTGC+CGG - chr4_2:47750675-47750694 Msa0603310:intron 60.0%
! AGATGCCGCGAAGAAGCACG+AGG - chr4_2:47751151-47751170 Msa0603310:intron 60.0%
!! CGGAGGCGCCGGAGATTGTT+CGG + chr4_2:47750194-47750213 None:intergenic 65.0%
GCCGTTGTCGACGGCGTTCA+TGG - chr4_2:47750233-47750252 Msa0603310:CDS 65.0%
CACTCCGGTGGCTCCTTCAC+CGG + chr4_2:47750666-47750685 None:intergenic 65.0%
CCGGCAAGAATCCACTCCGG+TGG + chr4_2:47750678-47750697 None:intergenic 65.0%
CGGTGAAGGAGCCACCGGAG+TGG - chr4_2:47750664-47750683 Msa0603310:intron 70.0%
!! GTTGGCGCTGACGGAGGCGC+CGG + chr4_2:47750205-47750224 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 47749792 47753244 47749792 ID=Msa0603310;Name=Msa0603310
chr4_2 mRNA 47749792 47753244 47749792 ID=Msa0603310-mRNA-1;Parent=Msa0603310;Name=Msa0603310-mRNA-1;_AED=0.02;_eAED=0.02;_QI=211|1|1|1|1|1|4|364|710
chr4_2 exon 47749792 47750271 47749792 ID=Msa0603310-mRNA-1:exon:10919;Parent=Msa0603310-mRNA-1
chr4_2 exon 47750392 47750656 47750392 ID=Msa0603310-mRNA-1:exon:10918;Parent=Msa0603310-mRNA-1
chr4_2 exon 47751196 47751309 47751196 ID=Msa0603310-mRNA-1:exon:10917;Parent=Msa0603310-mRNA-1
chr4_2 exon 47751396 47753244 47751396 ID=Msa0603310-mRNA-1:exon:10916;Parent=Msa0603310-mRNA-1
chr4_2 five_prime_UTR 47753034 47753244 47753034 ID=Msa0603310-mRNA-1:five_prime_utr;Parent=Msa0603310-mRNA-1
chr4_2 CDS 47751396 47753033 47751396 ID=Msa0603310-mRNA-1:cds;Parent=Msa0603310-mRNA-1
chr4_2 CDS 47751196 47751309 47751196 ID=Msa0603310-mRNA-1:cds;Parent=Msa0603310-mRNA-1
chr4_2 CDS 47750392 47750656 47750392 ID=Msa0603310-mRNA-1:cds;Parent=Msa0603310-mRNA-1
chr4_2 CDS 47750156 47750271 47750156 ID=Msa0603310-mRNA-1:cds;Parent=Msa0603310-mRNA-1
chr4_2 three_prime_UTR 47749792 47750155 47749792 ID=Msa0603310-mRNA-1:three_prime_utr;Parent=Msa0603310-mRNA-1
Gene Sequence

>Msa0603310

ATGGGAAACAAAATAGGAAAATTAACGGTATGTTTCACCGGAAACAACAACAGTAACCGTAAACACGACATATCCATATTAATAACGGATCCTTTAGATGAAGGACTTGGTCACTCTTTCTGTTACGTTAGACCCGACCCGACCCGTTTATCTTCTTCCAAAGTTCACTCTGAAGAAACCACAACTTTCCGAACAATCTCCGGCGCCTCCGTCAGCGCCAACACTTCAACGCCGTTGTCGACGGCGTTCATGGATTTATACTCCTACGGTTGTTTTGATAGAGCTGCAGCTTTTGAAAGTTCAACTTCTTTTGCTTCTCTTCCGTTGCAACCGATTCCGAAGGCTTTTTCCGGTAACTTTGGTGGTGGTGGTGGTGGTTTTCCGAGTTCCGGTCCGTTAGAGCGTGGGTTTATGTCCGGTCCGATTGAGCGTGGGTTCATGTCCGGTCCGATTGATCGTGGTTTATTTTCCGGTCCGATGGATAAAGATAGCTCCGGCACCGGCGTTGATCAGTTTCAGAGAAGCTTCTCTCATAGTGGATTAGGTTTTTCGGTTCGACCAAGATCGAGGAAAGAAAAATGGATTCGTGTTCTTCAACGTGCGATTTCGAAGACTCTGTCACGTGGACAGGGTTCTATTGTTACTCCGATCAAAGGTGTACCGGTGAAGGAGCCACCGGAGTGGATTCTTGCCGGTGAGAAGCACAATGAGAATTTGACTGTGAGTAGTTTGAATTTGAGTAGTGAAGGTAGTTTGGAAGATGATGATTCGTTGGGAAGTCAGAATCTTCAATGGGCTCAAGGGAAAGCAGGGGAAGATCGTGTTCATGTTGTTGTTTCTGAGGAACATGGTTGGGTTTTTGTTGGGATTTATGATGGTTTTAATGGTCCTGATGCACCTGATTATTTGTTGTCAAATCTTTATAGTTTTGTTCATAAGGAGCTTAAAGGGTTGCTTTGGGATGATGGGGTTTCATCGGATAATTCGAATTTGAAACCGCGTGATTCGGTTGATGATGATGTTGTTGATGGTGTTGTTGTGAAAGAGGATATGTTGTTTGATGATTGTTCTCAATGTGTTGATCAAGAGAAAGGTGATTCGATTTCAAAGAAAAAAAAGAGAGGTAAGAATTCGAAGAATAAGTACAAAGATGCCGCGAAGAAGCACGAGGAGAATCAAAGGAGATGGAAGTGTGAATGGGATAGGGAGAGATTAGAGCTTGATAGAAGATTGAAGGAGCAATTAAGTGGCGGTGATGCCGACGATAATTCTGTTAATCATTCAGATGTTTTGGAAGCTCTTTCGCGAGCTTTGAGGAAAACCGAGGAGTCTTATCTTGATGTTGCGGATAAGATGGTGATGGAGAATCCGGAACTTGCTTTAATGGGTTCATGTGTTCTTGTAATGTTGATGAAAGGGGAAGATGTTTATGTGATGAATGTGGGGGATAGTAGAGCTGTTTTGGCACAAAAAGCCGAACCTGATTATTGGATCGGGAAAATTAGACAGGATTTGGAGAGGATTAATGAAGAGACAATGCATGATCTTGAATCATGGGATGATGCTGATAAATCAAATGTTGTACCTAGTTTAAGTGCTTTTCAGCTTACCAAGGATCATAGTACCAATGTTGAAGAGGAAGTAATCAGAATAATAAAAGAGCACCCAGATGATCCTTGTGCTGTGATCAATGATCGTGTTAAGGGATCTCTCAAGGTTACTCGAGCATTTGGTGCCGGATTTCTCAAACAGCCCAAGTGGAACAATGCACTTCTGGAGATGTTTAGAATAGACTACGTAGGAAATTCTCCATACATTACTTGCCAACCATATCTTAAACACCACAGATTAGGCCAAAAAGACAAATTTTTGATTCTATGCTCCGACGGACTCTATCAATACTTGTCAAACGAAGAAGCAGTAGCTGAAGTTGAGCTTTTCATCACATTACAACCTGAAGGAGACCCTGCTCAACATTTGGTCGAAGAAGTCTTGTTTCGCGCTGCAAAGAAAGCTGGTCTGGACTTTCATGAATTGCTCGAAATTCCACAAGGTGATAGGCGGCGTTACCACGACGATGTTTCCATCATTGTTATTTCTTTGGAAGGAAGGATATGGAGATCATGTGTATAA
Protein sequence

>Msa0603310

MGNKIGKLTVCFTGNNNSNRKHDISILITDPLDEGLGHSFCYVRPDPTRLSSSKVHSEETTTFRTISGASVSANTSTPLSTAFMDLYSYGCFDRAAAFESSTSFASLPLQPIPKAFSGNFGGGGGGFPSSGPLERGFMSGPIERGFMSGPIDRGLFSGPMDKDSSGTGVDQFQRSFSHSGLGFSVRPRSRKEKWIRVLQRAISKTLSRGQGSIVTPIKGVPVKEPPEWILAGEKHNENLTVSSLNLSSEGSLEDDDSLGSQNLQWAQGKAGEDRVHVVVSEEHGWVFVGIYDGFNGPDAPDYLLSNLYSFVHKELKGLLWDDGVSSDNSNLKPRDSVDDDVVDGVVVKEDMLFDDCSQCVDQEKGDSISKKKKRGKNSKNKYKDAAKKHEENQRRWKCEWDRERLELDRRLKEQLSGGDADDNSVNHSDVLEALSRALRKTEESYLDVADKMVMENPELALMGSCVLVMLMKGEDVYVMNVGDSRAVLAQKAEPDYWIGKIRQDLERINEETMHDLESWDDADKSNVVPSLSAFQLTKDHSTNVEEEVIRIIKEHPDDPCAVINDRVKGSLKVTRAFGAGFLKQPKWNNALLEMFRIDYVGNSPYITCQPYLKHHRLGQKDKFLILCSDGLYQYLSNEEAVAEVELFITLQPEGDPAQHLVEEVLFRAAKKAGLDFHELLEIPQGDRRRYHDDVSIIVISLEGRIWRSCV*