AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0548420

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0548420 MtrunA17_Chr4g0031521 97.616 713 8 3 1 713 1 704 0.0 1420
Msa0548420 MtrunA17_Chr4g0054811 39.239 841 314 21 1 711 1 774 3.94e-165 494
Msa0548420 MtrunA17_Chr7g0238501 56.721 305 93 3 409 713 290 555 3.07e-110 345
Msa0548420 MtrunA17_Chr7g0238501 30.790 367 185 8 1 348 1 317 1.85e-37 147
Msa0548420 MtrunA17_Chr1g0193691 51.786 336 139 2 399 711 555 890 1.98e-109 352
Msa0548420 MtrunA17_Chr1g0193691 34.747 495 231 17 1 445 1 453 3.47e-60 218
Msa0548420 MtrunA17_Chr4g0039061 57.807 301 89 3 413 713 229 491 2.89e-108 337
Msa0548420 MtrunA17_Chr4g0039061 67.188 64 21 0 261 324 173 236 6.30e-23 103
Msa0548420 MtrunA17_Chr4g0067301 40.809 272 133 1 433 704 244 487 5.38e-62 215
Msa0548420 MtrunA17_Chr4g0067301 49.254 67 34 0 258 324 132 198 3.55e-12 69.3
Msa0548420 MtrunA17_Chr2g0295991 40.074 272 133 2 433 704 235 476 6.44e-60 209
Msa0548420 MtrunA17_Chr2g0295991 47.945 73 38 0 261 333 137 209 1.09e-13 74.3
Msa0548420 MtrunA17_Chr8g0371321 31.786 280 149 5 434 708 115 357 5.75e-36 140
Msa0548420 MtrunA17_Chr1g0213351 31.228 285 154 5 426 705 108 355 9.83e-36 139
Msa0548420 MtrunA17_Chr1g0181901 30.693 303 147 6 436 708 114 383 1.12e-32 130
Msa0548420 MtrunA17_Chr3g0089691 32.333 300 142 12 421 705 8 261 7.84e-31 123
Msa0548420 MtrunA17_Chr7g0263281 30.824 279 151 6 435 708 108 349 8.88e-31 124
Msa0548420 MtrunA17_Chr7g0250061 28.214 280 159 5 434 708 112 354 1.54e-30 124
Msa0548420 MtrunA17_Chr3g0089711 31.849 292 141 11 429 705 99 347 5.17e-30 122
Msa0548420 MtrunA17_Chr3g0089721 31.849 292 141 11 429 705 99 347 6.83e-30 122
Msa0548420 MtrunA17_Chr1g0211251 28.929 280 157 5 434 708 117 359 1.66e-28 118
Msa0548420 MtrunA17_Chr2g0294291 27.636 275 157 5 435 704 113 350 2.69e-28 117
Msa0548420 MtrunA17_Chr7g0223641 30.588 255 108 6 458 704 3 196 4.42e-28 112
Msa0548420 MtrunA17_Chr1g0207571 30.686 277 150 7 435 705 98 338 1.67e-27 115
Msa0548420 MtrunA17_Chr1g0175381 27.208 283 161 8 437 713 118 361 1.93e-25 109
Msa0548420 MtrunA17_Chr7g0256751 31.834 289 139 10 429 702 100 345 7.74e-25 108
Msa0548420 MtrunA17_Chr7g0256771 30.992 242 112 10 418 648 95 292 1.71e-19 90.1
Msa0548420 MtrunA17_Chr4g0063051 26.772 254 124 7 458 709 120 313 5.32e-17 83.2
Msa0548420 MtrunA17_Chr7g0256761 28.022 182 109 6 534 709 20 185 1.35e-14 73.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0548420 AT1G07630.1 68.050 723 160 15 1 713 1 662 0.0 930
Msa0548420 AT2G28890.1 68.194 720 156 15 1 713 1 654 0.0 920
Msa0548420 AT5G02400.1 49.581 716 278 19 19 713 21 674 0.0 608
Msa0548420 AT3G09400.1 47.814 732 281 17 1 713 1 650 0.0 563
Msa0548420 AT3G09400.2 45.612 695 277 17 1 676 1 613 1.12e-165 491
Msa0548420 AT2G46920.3 47.970 394 158 9 357 711 457 842 4.35e-107 345
Msa0548420 AT2G46920.3 37.955 440 177 14 1 376 1 408 5.25e-68 240
Msa0548420 AT2G46920.1 47.970 394 158 9 357 711 457 842 4.35e-107 345
Msa0548420 AT2G46920.1 37.955 440 177 14 1 376 1 408 5.25e-68 240
Msa0548420 AT2G46920.2 47.970 394 158 9 357 711 457 842 4.35e-107 345
Msa0548420 AT2G46920.2 37.955 440 177 14 1 376 1 408 5.25e-68 240
Msa0548420 AT2G46920.4 47.970 394 158 9 357 711 457 842 4.35e-107 345
Msa0548420 AT2G46920.4 37.955 440 177 14 1 376 1 408 5.25e-68 240
Msa0548420 AT2G35350.1 53.605 319 118 7 410 711 473 778 7.22e-102 330
Msa0548420 AT2G35350.1 33.850 387 144 15 1 331 1 331 1.66e-39 156
Msa0548420 AT3G16560.1 37.821 312 156 3 393 704 207 480 1.46e-64 223
Msa0548420 AT3G16560.1 47.297 74 39 0 248 321 133 206 2.35e-12 70.5
Msa0548420 AT3G16560.4 37.821 312 156 3 393 704 207 480 1.46e-64 223
Msa0548420 AT3G16560.4 47.297 74 39 0 248 321 133 206 2.35e-12 70.5
Msa0548420 AT3G16560.2 37.821 312 156 3 393 704 207 480 1.46e-64 223
Msa0548420 AT3G16560.2 47.297 74 39 0 248 321 133 206 2.35e-12 70.5
Msa0548420 AT3G16560.3 35.915 284 144 3 393 676 207 452 1.30e-51 186
Msa0548420 AT3G16560.3 47.297 74 39 0 248 321 133 206 2.07e-12 70.5
Msa0548420 AT3G55050.1 31.541 279 145 6 435 706 121 360 2.18e-34 135
Msa0548420 AT3G55050.2 31.541 279 145 6 435 706 121 360 2.18e-34 135
Msa0548420 AT3G12620.2 30.208 288 159 5 426 708 111 361 3.82e-34 135
Msa0548420 AT3G12620.1 30.208 288 159 5 426 708 111 361 3.82e-34 135
Msa0548420 AT3G51370.2 30.421 309 162 7 407 705 4 269 1.36e-33 131
Msa0548420 AT3G17090.1 30.545 275 148 4 435 704 122 358 1.81e-33 133
Msa0548420 AT4G38520.2 31.502 273 145 5 435 702 118 353 5.70e-33 132
Msa0548420 AT4G38520.1 31.502 273 145 5 435 702 118 353 5.70e-33 132
Msa0548420 AT5G66080.2 31.399 293 156 6 418 705 1 253 2.39e-32 127
Msa0548420 AT3G51370.1 30.325 277 151 4 434 705 115 354 3.05e-32 129
Msa0548420 AT3G51370.3 30.325 277 151 4 434 705 115 354 3.05e-32 129
Msa0548420 AT5G66080.1 31.769 277 147 5 434 705 118 357 1.21e-31 127
Msa0548420 AT5G02760.1 32.000 275 143 5 435 704 109 344 6.90e-30 122
Msa0548420 AT4G33920.2 29.603 277 156 5 434 705 14 256 1.87e-29 119
Msa0548420 AT4G33920.1 29.603 277 156 5 434 705 97 339 4.33e-29 120
Msa0548420 AT5G06750.2 29.032 279 159 5 435 708 37 281 1.74e-28 117
Msa0548420 AT5G06750.3 29.032 279 159 5 435 708 119 363 2.75e-28 118
Msa0548420 AT5G06750.1 29.032 279 159 5 435 708 119 363 2.75e-28 118
Msa0548420 AT4G33920.3 31.076 251 134 5 460 705 10 226 1.08e-26 110
Msa0548420 AT3G17090.2 31.050 219 112 3 435 648 122 306 2.41e-24 104

Find 202 sgRNAs with CRISPR-Local

Find 268 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAATCCGGAACTTGCTTTAA+TGG 0.204565 4_1:-42462081 Msa0548420:CDS
ACGGTTACTGTTGTTGTTTC+CGG 0.205809 4_1:+42463417 None:intergenic
AGAAAGCCGAACCTGATTAT+TGG 0.247572 4_1:-42461977 Msa0548420:CDS
TTCCATCATTGTTATTTCTT+TGG 0.250349 4_1:-42460613 Msa0548420:CDS
TGGGAAAATTAGACAGGATT+TGG 0.255176 4_1:-42461952 Msa0548420:CDS
CAAGTGGAACAATGCACTTC+TGG 0.269345 4_1:-42461060 Msa0548420:CDS
TAAAGGATCCGTTATTAATA+TGG 0.281615 4_1:+42463381 None:intergenic
TTTGTTATGGGAAACAAAAT+AGG 0.293503 4_1:-42463460 Msa0548420:exon
TGTTAATCATTCAGATGTTT+TGG 0.294451 4_1:-42462174 Msa0548420:CDS
AGCTTCTCTCATAGTGGATT+AGG 0.296940 4_1:-42462923 Msa0548420:CDS
TTTGGTGGTGTTGGTGGTGG+TGG 0.324321 4_1:-42463097 Msa0548420:CDS
GCTTCTCTCATAGTGGATTA+GGG 0.330402 4_1:-42462922 Msa0548420:CDS
GGGAGATAGTAGAGCTGTTT+TGG 0.332474 4_1:-42462003 Msa0548420:CDS
TTTGTTCATAAGGAGCTTAA+AGG 0.334724 4_1:-42462518 Msa0548420:CDS
ATATGGATATGTCGTGTTTA+CGG 0.335656 4_1:+42463398 None:intergenic
GCCGTTGTCGACGGCGTTCA+TGG 0.337832 4_1:-42463224 Msa0548420:CDS
AAAGGGTTGCTTTGGGATGA+TGG 0.341522 4_1:-42462500 Msa0548420:CDS
TTTGAGTAGTGAAGGTAGTT+TGG 0.342225 4_1:-42462711 Msa0548420:CDS
AGGAGCTTAAAGGGTTGCTT+TGG 0.344311 4_1:-42462508 Msa0548420:CDS
TATATTATGATGATGATATA+AGG 0.344834 4_1:+42460543 None:intergenic
TTTCTTTGGAAGGAAGGATA+TGG 0.347821 4_1:-42460599 Msa0548420:CDS
GGTGGTGTTGGTGGTGGTGG+TGG 0.349063 4_1:-42463094 Msa0548420:CDS
AATCCGGAACTTGCTTTAAT+GGG 0.351962 4_1:-42462080 Msa0548420:CDS
GTTGATGATGATGTTGTTGA+TGG 0.361250 4_1:-42462437 Msa0548420:CDS
TGTTGAGCAGGGTCTCCTTC+AGG 0.362431 4_1:+42460861 None:intergenic
CAGAAGTGCATTGTTCCACT+TGG 0.376775 4_1:+42461061 None:intergenic
AAGGGTTGCTTTGGGATGAT+GGG 0.382657 4_1:-42462499 Msa0548420:CDS
GTGGTCAACGATCGTGTTAA+AGG 0.382777 4_1:-42461676 Msa0548420:CDS
TAGTTCTTGTTCCTAGTACT+TGG 0.392726 4_1:-42463543 Msa0548420:five_prime_UTR
GAATTTGAAACCGCGTGATT+CGG 0.400287 4_1:-42462459 Msa0548420:CDS
ACCAACACCACCAAAGTTAC+CGG 0.401059 4_1:+42463105 None:intergenic
GAAAATAAACCACGATCAAT+CGG 0.401422 4_1:+42462998 None:intergenic
CTCAAGGTTACTCGAGCATT+TGG 0.402324 4_1:-42461649 Msa0548420:CDS
CCCAATCCAATAATCAGGTT+CGG 0.403305 4_1:+42461971 None:intergenic
TTGTTCATAAGGAGCTTAAA+GGG 0.406793 4_1:-42462517 Msa0548420:CDS
CCACCGGAGTGGATTCTTGC+CGG 0.406897 4_1:-42462773 Msa0548420:CDS
TCGAAATTCCACAAGGTGAT+AGG 0.408336 4_1:-42460656 Msa0548420:CDS
GAAGATAAACGGGTCGGGTC+GGG 0.411687 4_1:+42463322 None:intergenic
GCCGAACCTGATTATTGGAT+TGG 0.412405 4_1:-42461972 Msa0548420:CDS
TATAATAATAAACAAAATCC+AGG 0.412450 4_1:+42460283 None:intergenic
AGGAGACCCTGCTCAACATT+TGG 0.412890 4_1:-42460856 Msa0548420:CDS
GAACTTTGGAAGAAGATAAA+CGG 0.420518 4_1:+42463311 None:intergenic
AAAGGAGATGGAAGTGTGAA+TGG 0.425538 4_1:-42462268 Msa0548420:CDS
AATCTGTGGTGTTTAAGATA+TGG 0.429109 4_1:+42460987 None:intergenic
GAACCCATTAAAGCAAGTTC+CGG 0.431709 4_1:+42462077 None:intergenic
GATGGAAGTGTGAATGGGAT+AGG 0.433001 4_1:-42462262 Msa0548420:CDS
AAGCTCTTTCGCGAGCTTTG+AGG 0.433880 4_1:-42462151 Msa0548420:CDS
GGAGCTTAAAGGGTTGCTTT+GGG 0.434258 4_1:-42462507 Msa0548420:CDS
AACAAATAATCAGGTGCATC+AGG 0.434263 4_1:+42462557 None:intergenic
AACTTTGGTGGTGTTGGTGG+TGG 0.437354 4_1:-42463100 Msa0548420:CDS
ATTCTCCTCGTGCTTCTTCG+CGG 0.439380 4_1:+42462292 None:intergenic
CGGAGGCGCCGGAGATTGTT+CGG 0.441737 4_1:+42463266 None:intergenic
GACATGAACCCACGCTCAAT+CGG 0.443578 4_1:+42463025 None:intergenic
ACCACGGCACAAGGATCATC+CGG 0.449572 4_1:+42461694 None:intergenic
CAATGGGCTCAAGGGAAAGC+AGG 0.451112 4_1:-42462656 Msa0548420:CDS
CAATGCATGATCTTGAATCA+TGG 0.452610 4_1:-42461911 Msa0548420:CDS
TCCGGTAACTTTGGTGGTGT+TGG 0.452955 4_1:-42463106 Msa0548420:CDS
CCGAACCTGATTATTGGATT+GGG 0.454169 4_1:-42461971 Msa0548420:CDS
TTCCAAAGAAATAACAATGA+TGG 0.456946 4_1:+42460611 None:intergenic
AGATTTGACAACAAATAATC+AGG 0.457442 4_1:+42462548 None:intergenic
TTGGATTGGGAAAATTAGAC+AGG 0.458394 4_1:-42461958 Msa0548420:CDS
AGAGCTTGATAGAAGATTGA+AGG 0.459767 4_1:-42462231 Msa0548420:CDS
AAAACTTACCTCTTCAACAT+TGG 0.461053 4_1:+42461821 None:intergenic
TGGTGCCGGATTTCTCAAAC+AGG 0.467257 4_1:-42461629 Msa0548420:intron
TATCTTAAACACCACAGATT+AGG 0.469227 4_1:-42460984 Msa0548420:CDS
AAACTGATCAACGCCGGTGC+CGG 0.470846 4_1:+42462952 None:intergenic
AACTTTGGAAGAAGATAAAC+GGG 0.472058 4_1:+42463312 None:intergenic
TGTTTCTGAGGAACATGGTT+GGG 0.473614 4_1:-42462612 Msa0548420:CDS
TAAGATGGTGATGGAGAATC+CGG 0.475384 4_1:-42462096 Msa0548420:CDS
TTTGGAAGATGATGATTCGT+TGG 0.476363 4_1:-42462693 Msa0548420:CDS
ATAACGGATCCTTTAGATGA+AGG 0.479407 4_1:-42463373 Msa0548420:CDS
TTCTTGTAATGTTGATGAAA+GGG 0.479413 4_1:-42462049 Msa0548420:CDS
TTATGATGATGATATAAGGT+TGG 0.479889 4_1:+42460547 None:intergenic
TCTTCGACCAAATGTTGAGC+AGG 0.483562 4_1:+42460849 None:intergenic
TTCACCGCCACACCTTTGAT+CGG 0.483901 4_1:+42462800 None:intergenic
TGACCAAGTCCTTCATCTAA+AGG 0.485372 4_1:+42463364 None:intergenic
GGATTTGGAGAGGATTAATG+AGG 0.485566 4_1:-42461937 Msa0548420:CDS
TATGGTTGGCAAGTAATGTA+TGG 0.487207 4_1:+42461005 None:intergenic
ATCATTGTTATTTCTTTGGA+AGG 0.487899 4_1:-42460609 Msa0548420:CDS
AAACAAAATAGGAAAATTAA+CGG 0.488093 4_1:-42463449 Msa0548420:CDS
CAGAGAAGCTTCTCTCATAG+TGG 0.489328 4_1:-42462929 Msa0548420:CDS
TTGAAAGATTAGTGGGAACT+AGG 0.489496 4_1:+42463631 None:intergenic
TTGTTATTTCTTTGGAAGGA+AGG 0.491846 4_1:-42460605 Msa0548420:CDS
AACATTGGTACTATGATCCT+TGG 0.492918 4_1:+42461836 None:intergenic
TTCATGGATTTATACTCCTA+CGG 0.494403 4_1:-42463208 Msa0548420:CDS
TTGGGATGATGGGGTTTCAT+CGG 0.500864 4_1:-42462489 Msa0548420:CDS
AGAAGATAAACGGGTCGGGT+CGG 0.501483 4_1:+42463321 None:intergenic
AAATTAACGGTATGTTTCAC+CGG 0.503917 4_1:-42463436 Msa0548420:CDS
ACGAACCTGTTTGAGAAATC+CGG 0.503935 4_1:+42461624 None:intergenic
ATTGAGCGTGGGTTCATGTC+CGG 0.505314 4_1:-42463022 Msa0548420:CDS
AGGGTTGCTTTGGGATGATG+GGG 0.507169 4_1:-42462498 Msa0548420:CDS
GGTAACTTTGGTGGTGTTGG+TGG 0.507182 4_1:-42463103 Msa0548420:CDS
CGTTGAAGTGTTGGCGCTGA+CGG 0.507965 4_1:+42463246 None:intergenic
AGTTTGAATTTGAGTAGTGA+AGG 0.511209 4_1:-42462719 Msa0548420:CDS
ACAACTTTCCGAACAATCTC+CGG 0.511363 4_1:-42463274 Msa0548420:CDS
CGCTCTAACGGACCGGAACT+CGG 0.513564 4_1:+42463064 None:intergenic
TCCGTTGCAACCGATTCCGA+AGG 0.513883 4_1:-42463134 Msa0548420:CDS
TGATGGTGTTGTTGTGAAAG+AGG 0.514346 4_1:-42462420 Msa0548420:CDS
TTAGAGCGTGGGTTTATGTC+CGG 0.514791 4_1:-42463049 Msa0548420:CDS
CGACATATCCATATTAATAA+CGG 0.515144 4_1:-42463389 Msa0548420:CDS
GATCAAAGGTGTGGCGGTGA+AGG 0.515393 4_1:-42462798 Msa0548420:CDS
GATAAAGATTGCTCCGGCAC+CGG 0.515415 4_1:-42462965 Msa0548420:CDS
AAGGAGATGGAAGTGTGAAT+GGG 0.515548 4_1:-42462267 Msa0548420:CDS
GTTCCGGTCCGTTAGAGCGT+GGG 0.516393 4_1:-42463060 Msa0548420:CDS
TCGTTTATATTTAAATTAGG+AGG 0.518447 4_1:-42460353 Msa0548420:three_prime_UTR
GACATAAACCCACGCTCTAA+CGG 0.522963 4_1:+42463052 None:intergenic
TTGGAAGATGATGATTCGTT+GGG 0.524167 4_1:-42462692 Msa0548420:CDS
ATGGAAGTGTGAATGGGATA+GGG 0.524574 4_1:-42462261 Msa0548420:CDS
GGTTTCTTCAGAGTGAACTT+TGG 0.525914 4_1:+42463297 None:intergenic
TGGGAAGTCAGAATCTTCAA+TGG 0.526449 4_1:-42462673 Msa0548420:CDS
ATGAATAGAAACCAAGTACT+AGG 0.526919 4_1:+42463532 None:intergenic
AGAAGCACGAGGAGAATCAA+AGG 0.529931 4_1:-42462286 Msa0548420:CDS
TTTCGCGCTGCAAAGAAAGC+TGG 0.530460 4_1:-42460819 Msa0548420:intron
TGAAGTGTTGGCGCTGACGG+AGG 0.531029 4_1:+42463249 None:intergenic
GATGTTTATGTGATGAATGT+GGG 0.536014 4_1:-42462023 Msa0548420:CDS
CGATCGTTGACCACGGCACA+AGG 0.537467 4_1:+42461685 None:intergenic
CAATGTGTTGATCAAGAGAA+AGG 0.538305 4_1:-42462374 Msa0548420:CDS
ATGTCCGGTCCGATTGAGCG+TGG 0.539933 4_1:-42463034 Msa0548420:CDS
AGAAGTGCATTGTTCCACTT+GGG 0.541493 4_1:+42461062 None:intergenic
GTTGGCGCTGACGGAGGCGC+CGG 0.543294 4_1:+42463255 None:intergenic
CGACAACGGCGTTGAAGTGT+TGG 0.543904 4_1:+42463237 None:intergenic
CACTTCAACGCCGTTGTCGA+CGG 0.543934 4_1:-42463233 Msa0548420:CDS
TCTATTGTTACTCCGATCAA+AGG 0.544710 4_1:-42462812 Msa0548420:CDS
AGATGTTTATGTGATGAATG+TGG 0.546822 4_1:-42462024 Msa0548420:CDS
TCTTGATGTTGCGGATAAGA+TGG 0.547525 4_1:-42462111 Msa0548420:CDS
GGGAAGTCAGAATCTTCAAT+GGG 0.550933 4_1:-42462672 Msa0548420:CDS
GCCTTCGGAATCGGTTGCAA+CGG 0.551003 4_1:+42463133 None:intergenic
GTTACTCGAGCATTTGGTGC+CGG 0.551671 4_1:-42461643 Msa0548420:CDS
AGTTCCGGTCCGTTAGAGCG+TGG 0.554284 4_1:-42463061 Msa0548420:CDS
CAGAATAATAAAAGAACACC+CGG 0.555589 4_1:-42461713 Msa0548420:CDS
CAGAATCTTCAATGGGCTCA+AGG 0.556444 4_1:-42462665 Msa0548420:CDS
TTCATCACATTACAACCTGA+AGG 0.557463 4_1:-42460876 Msa0548420:CDS
GAGAGAGATTGTGAAGTGAA+TGG 0.558486 4_1:+42463666 None:intergenic
TGGAAGAAGATAAACGGGTC+GGG 0.560735 4_1:+42463317 None:intergenic
GTTCTTGTAATGTTGATGAA+AGG 0.566141 4_1:-42462050 Msa0548420:CDS
GATCCTTTAGATGAAGGACT+TGG 0.568383 4_1:-42463367 Msa0548420:CDS
TGTCCGGTCCGATTGAGCGT+GGG 0.569809 4_1:-42463033 Msa0548420:CDS
GGAGATTGTTCGGAAAGTTG+TGG 0.572383 4_1:+42463276 None:intergenic
GTTATTGTTTGCAGCCCAAG+TGG 0.572427 4_1:-42461076 Msa0548420:intron
AATGGGCTCAAGGGAAAGCA+GGG 0.572972 4_1:-42462655 Msa0548420:CDS
TCCATGAACGCCGTCGACAA+CGG 0.574282 4_1:+42463223 None:intergenic
AAATTAGACAGGATTTGGAG+AGG 0.574734 4_1:-42461947 Msa0548420:CDS
AAACCCACGCTCTAACGGAC+CGG 0.575313 4_1:+42463057 None:intergenic
TGTTCATGTTGTTGTTTCTG+AGG 0.576807 4_1:-42462624 Msa0548420:CDS
AATGCATGATCTTGAATCAT+GGG 0.577709 4_1:-42461910 Msa0548420:CDS
AGAATCTTCAATGGGCTCAA+GGG 0.581264 4_1:-42462664 Msa0548420:CDS
GTTGTTGTTTCTGAGGAACA+TGG 0.582318 4_1:-42462617 Msa0548420:CDS
TGTGGCGGTGAAGGAGCCAC+CGG 0.582479 4_1:-42462789 Msa0548420:CDS
GAACCCACGCTCAATCGGAC+CGG 0.583850 4_1:+42463030 None:intergenic
CCGGAGCAATCTTTATCCAT+CGG 0.583904 4_1:+42462971 None:intergenic
TTTCGGTTCGACCAAGATCG+AGG 0.584381 4_1:-42462898 Msa0548420:CDS
TTGTTTCTGAGGAACATGGT+TGG 0.588748 4_1:-42462613 Msa0548420:CDS
GGAGTAATTATGTAAATCAG+TGG 0.593753 4_1:-42460332 Msa0548420:three_prime_UTR
ACGAGGAGAATCAAAGGAGA+TGG 0.596338 4_1:-42462280 Msa0548420:CDS
AGCTGAAAAGCACTTAAACT+AGG 0.596987 4_1:+42461861 None:intergenic
ATGTAAATCAGTGGATAGTG+AGG 0.597119 4_1:-42460323 Msa0548420:three_prime_UTR
TCATAGTACCAATGTTGAAG+AGG 0.597252 4_1:-42461829 Msa0548420:intron
ATTCTCATTATGCTTCTCAC+CGG 0.598472 4_1:+42462754 None:intergenic
ATGTCCGGTCCGATTGATCG+TGG 0.599187 4_1:-42463007 Msa0548420:CDS
AACATCAAGATAAGACTCCT+CGG 0.601800 4_1:+42462124 None:intergenic
TCTTGTAATGTTGATGAAAG+GGG 0.604649 4_1:-42462048 Msa0548420:CDS
AGATTGAAGGAGCAATTAAG+TGG 0.604819 4_1:-42462218 Msa0548420:CDS
TTGGAAGAAGATAAACGGGT+CGG 0.606093 4_1:+42463316 None:intergenic
GCAATCTTTATCCATCGGAC+CGG 0.606492 4_1:+42462976 None:intergenic
CTTCGACCAAATGTTGAGCA+GGG 0.606676 4_1:+42460850 None:intergenic
ATAATAATAAACAAAATCCA+GGG 0.607632 4_1:+42460284 None:intergenic
GATTGTAAAGTTGTAAACCC+TGG 0.609142 4_1:-42460301 Msa0548420:three_prime_UTR
TCGACACTCGTGAATAGCGA+GGG 0.610488 4_1:+42460458 None:intergenic
CCCGGATGATCCTTGTGCCG+TGG 0.620027 4_1:-42461695 Msa0548420:CDS
CCGGCAAGAATCCACTCCGG+TGG 0.621535 4_1:+42462773 None:intergenic
TCGTGTTAAAGGATCTCTCA+AGG 0.624885 4_1:-42461665 Msa0548420:CDS
TGTGGTGTTTAAGATATGGT+TGG 0.627286 4_1:+42460991 None:intergenic
GGAGTCTTATCTTGATGTTG+CGG 0.628468 4_1:-42462120 Msa0548420:CDS
ATGAGATATATGCTGCCGAA+GGG 0.630709 4_1:-42460505 Msa0548420:three_prime_UTR
TATGAGATATATGCTGCCGA+AGG 0.630877 4_1:-42460506 Msa0548420:three_prime_UTR
TAACGCCGCCTATCACCTTG+TGG 0.632464 4_1:+42460648 None:intergenic
CGGTGAAGGAGCCACCGGAG+TGG 0.633574 4_1:-42462784 Msa0548420:CDS
CAAGTATTGATAGAGTCCGT+CGG 0.633991 4_1:+42460932 None:intergenic
CCACGGCACAAGGATCATCC+GGG 0.636074 4_1:+42461695 None:intergenic
CCGATGGATAAAGATTGCTC+CGG 0.640588 4_1:-42462971 Msa0548420:CDS
TAAACCACGATCAATCGGAC+CGG 0.640729 4_1:+42463003 None:intergenic
TGTTGCGGATAAGATGGTGA+TGG 0.644164 4_1:-42462105 Msa0548420:CDS
CTCGACACTCGTGAATAGCG+AGG 0.645488 4_1:+42460457 None:intergenic
TACTCCGATCAAAGGTGTGG+CGG 0.647922 4_1:-42462804 Msa0548420:CDS
ATGATTAACAGAATTATCGT+CGG 0.649688 4_1:+42462187 None:intergenic
GCGAGCTTTGAGGAAAACCG+AGG 0.665283 4_1:-42462141 Msa0548420:CDS
AGATGCCGCGAAGAAGCACG+AGG 0.671726 4_1:-42462297 Msa0548420:CDS
AAATTCCACAAGGTGATAGG+CGG 0.673197 4_1:-42460653 Msa0548420:CDS
TGTTACTCCGATCAAAGGTG+TGG 0.675105 4_1:-42462807 Msa0548420:CDS
AGCTCTATCAAAACAACCGT+AGG 0.682369 4_1:+42463192 None:intergenic
TCACCGGCAAGAATCCACTC+CGG 0.682589 4_1:+42462770 None:intergenic
TTTAACACGATCGTTGACCA+CGG 0.685294 4_1:+42461678 None:intergenic
ATTTCGAAGACTCTGTCACG+TGG 0.685701 4_1:-42462842 Msa0548420:CDS
TTGAAGGAGCAATTAAGTGG+CGG 0.690975 4_1:-42462215 Msa0548420:CDS
TCTCTGAAACTGATCAACGC+CGG 0.696982 4_1:+42462946 None:intergenic
CGACACTCGTGAATAGCGAG+GGG 0.698659 4_1:+42460459 None:intergenic
ATGTTTAGAATAGACTACGT+AGG 0.701650 4_1:-42461035 Msa0548420:CDS
CAATGATGGAAACATCGTCG+TGG 0.706639 4_1:+42460625 None:intergenic
ATGGGCTCAAGGGAAAGCAG+GGG 0.712223 4_1:-42462654 Msa0548420:CDS
TCATCATCAACCGAATCACG+CGG 0.743225 4_1:+42462449 None:intergenic
GAATTGCTCGAAATTCCACA+AGG 0.753407 4_1:-42460663 Msa0548420:CDS
GACACTCGTGAATAGCGAGG+GGG 0.766486 4_1:+42460460 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGTTTTTTAATTTTTGTTAT+GGG - chr4_1:42460412-42460431 Msa0548420:three_prime_UTR 10.0%
!!! TTTTCGTTTATATTTAAATT+AGG - chr4_1:42463528-42463547 Msa0548420:five_prime_UTR 10.0%
!!! GTGTTTTTTAATTTTTGTTA+TGG - chr4_1:42460411-42460430 Msa0548420:three_prime_UTR 15.0%
!! AAACAAAATAGGAAAATTAA+CGG - chr4_1:42460435-42460454 Msa0548420:three_prime_UTR 15.0%
!!! ATTAATTTTAAGAAACTCAA+CGG - chr4_1:42462325-42462344 Msa0548420:CDS 15.0%
!!! TATATTATGATGATGATATA+AGG + chr4_1:42463344-42463363 None:intergenic 15.0%
!!! TTTTTGCTTATATTTAAGTT+CGG + chr4_1:42463466-42463485 None:intergenic 15.0%
!! ATAATAATAAACAAAATCCA+GGG + chr4_1:42463603-42463622 None:intergenic 15.0%
!! TATAATAATAAACAAAATCC+AGG + chr4_1:42463604-42463623 None:intergenic 15.0%
!!! TCTTTATAGTTTTGTTCATA+AGG - chr4_1:42461356-42461375 Msa0548420:intron 20.0%
!! ATTTCAAAGAAAAAAAAGAG+AGG - chr4_1:42461540-42461559 Msa0548420:intron 20.0%
!! AAGTGTTTGATTATAATTTC+AGG - chr4_1:42462141-42462160 Msa0548420:CDS 20.0%
!! GTTGAGTTTCTTAAAATTAA+TGG + chr4_1:42462326-42462345 None:intergenic 20.0%
!! TTATTAATCTTCTACAAGAA+TGG + chr4_1:42462544-42462563 None:intergenic 20.0%
!! TTCTTTCCTTTCATAAAAAA+TGG - chr4_1:42462587-42462606 Msa0548420:CDS 20.0%
!!! AATGTTCCATTTTTTATGAA+AGG + chr4_1:42462596-42462615 None:intergenic 20.0%
!!! GAATCAAAAATTTGTCTTTT+TGG + chr4_1:42462926-42462945 None:intergenic 20.0%
!! TATTCTTCTTAATCTTAATC+AGG - chr4_1:42463185-42463204 Msa0548420:CDS 20.0%
!! TCGTTTATATTTAAATTAGG+AGG - chr4_1:42463531-42463550 Msa0548420:five_prime_UTR 20.0%
!!! ATGAAAAGATTTTTAAGGAA+TGG + chr4_1:42463666-42463685 None:intergenic 20.0%
!! TTTTGTGTTGAAAGATTAGT+GGG + chr4_1:42460263-42460282 None:intergenic 25.0%
!!! ATAACTCGTTTTTCATGTTT+TGG + chr4_1:42460286-42460305 None:intergenic 25.0%
!! GAACTATGAAACAAGTTTTA+AGG + chr4_1:42460327-42460346 None:intergenic 25.0%
!! TTTGTTATGGGAAACAAAAT+AGG - chr4_1:42460424-42460443 Msa0548420:three_prime_UTR 25.0%
! CGACATATCCATATTAATAA+CGG - chr4_1:42460495-42460514 Msa0548420:three_prime_UTR 25.0%
! TAAAGGATCCGTTATTAATA+TGG + chr4_1:42460506-42460525 None:intergenic 25.0%
! AGATTTGACAACAAATAATC+AGG + chr4_1:42461339-42461358 None:intergenic 25.0%
! ATGATTAACAGAATTATCGT+CGG + chr4_1:42461700-42461719 None:intergenic 25.0%
!! TGTTAATCATTCAGATGTTT+TGG - chr4_1:42461710-42461729 Msa0548420:CDS 25.0%
!! TTCTTGTAATGTTGATGAAA+GGG - chr4_1:42461835-42461854 Msa0548420:CDS 25.0%
!! AATCATATATCCTATCCTTT+TGG + chr4_1:42462448-42462467 None:intergenic 25.0%
! TGAAAATCCATTAAAGAGTT+AGG + chr4_1:42462623-42462642 None:intergenic 25.0%
! TAACATTTGTAGTTAGAGTT+AGG + chr4_1:42462792-42462811 None:intergenic 25.0%
! GCAATTGTGAAATCTTAAAA+TGG + chr4_1:42463102-42463121 None:intergenic 25.0%
! TTCTTAATCTTAATCAGGTT+TGG - chr4_1:42463190-42463209 Msa0548420:CDS 25.0%
!!! TTCCATCATTGTTATTTCTT+TGG - chr4_1:42463271-42463290 Msa0548420:CDS 25.0%
! TTCCAAAGAAATAACAATGA+TGG + chr4_1:42463276-42463295 None:intergenic 25.0%
!!! ATCATTGTTATTTCTTTGGA+AGG - chr4_1:42463275-42463294 Msa0548420:CDS 25.0%
!! TTATGATGATGATATAAGGT+TGG + chr4_1:42463340-42463359 None:intergenic 25.0%
! GTTTTGTGTTGAAAGATTAG+TGG + chr4_1:42460264-42460283 None:intergenic 30.0%
ATGAATAGAAACCAAGTACT+AGG + chr4_1:42460355-42460374 None:intergenic 30.0%
AAATTAACGGTATGTTTCAC+CGG - chr4_1:42460448-42460467 Msa0548420:three_prime_UTR 30.0%
ATATGGATATGTCGTGTTTA+CGG + chr4_1:42460489-42460508 None:intergenic 30.0%
! AACTTTGGAAGAAGATAAAC+GGG + chr4_1:42460575-42460594 None:intergenic 30.0%
! GAACTTTGGAAGAAGATAAA+CGG + chr4_1:42460576-42460595 None:intergenic 30.0%
TTCATGGATTTATACTCCTA+CGG - chr4_1:42460676-42460695 Msa0548420:CDS 30.0%
GAAAATAAACCACGATCAAT+CGG + chr4_1:42460889-42460908 None:intergenic 30.0%
!! ATTGATCGTGGTTTATTTTC+CGG - chr4_1:42460889-42460908 Msa0548420:CDS 30.0%
AGTTTGAATTTGAGTAGTGA+AGG - chr4_1:42461165-42461184 Msa0548420:intron 30.0%
! GTTTTTGTTGGGATTTATGA+TGG - chr4_1:42461294-42461313 Msa0548420:intron 30.0%
!!! GGGATTTATGATGGTTTTAA+TGG - chr4_1:42461303-42461322 Msa0548420:intron 30.0%
TTTGTTCATAAGGAGCTTAA+AGG - chr4_1:42461366-42461385 Msa0548420:intron 30.0%
TTGTTCATAAGGAGCTTAAA+GGG - chr4_1:42461367-42461386 Msa0548420:intron 30.0%
! GTTCTTGTAATGTTGATGAA+AGG - chr4_1:42461834-42461853 Msa0548420:CDS 30.0%
! TCTTGTAATGTTGATGAAAG+GGG - chr4_1:42461836-42461855 Msa0548420:CDS 30.0%
AGATGTTTATGTGATGAATG+TGG - chr4_1:42461860-42461879 Msa0548420:CDS 30.0%
GATGTTTATGTGATGAATGT+GGG - chr4_1:42461861-42461880 Msa0548420:CDS 30.0%
! ATTTTCCCAATCCAATAATC+AGG + chr4_1:42461921-42461940 None:intergenic 30.0%
AATGCATGATCTTGAATCAT+GGG - chr4_1:42461974-42461993 Msa0548420:CDS 30.0%
AAAACTTACCTCTTCAACAT+TGG + chr4_1:42462066-42462085 None:intergenic 30.0%
CAGAATAATAAAAGAACACC+CGG - chr4_1:42462171-42462190 Msa0548420:CDS 30.0%
!! ATTTTAAGAAACTCAACGGA+AGG - chr4_1:42462329-42462348 Msa0548420:CDS 30.0%
! ATATCCTATCCTTTTGGATT+TGG + chr4_1:42462442-42462461 None:intergenic 30.0%
! ATTTGTTTCTCTTGTCACAA+AGG - chr4_1:42462501-42462520 Msa0548420:CDS 30.0%
ATTGACACCTAACTCTTTAA+TGG - chr4_1:42462613-42462632 Msa0548420:CDS 30.0%
TGTCATATTAATGCTAAGCA+TGG - chr4_1:42462647-42462666 Msa0548420:CDS 30.0%
AACAGAAAATGTTTCACACT+TGG - chr4_1:42462717-42462736 Msa0548420:CDS 30.0%
! ATGTTTAGAATAGACTACGT+AGG - chr4_1:42462849-42462868 Msa0548420:CDS 30.0%
! AATCTGTGGTGTTTAAGATA+TGG + chr4_1:42462900-42462919 None:intergenic 30.0%
TATCTTAAACACCACAGATT+AGG - chr4_1:42462900-42462919 Msa0548420:CDS 30.0%
!! TTGTTATTTCTTTGGAAGGA+AGG - chr4_1:42463279-42463298 Msa0548420:CDS 30.0%
GGAGTAATTATGTAAATCAG+TGG - chr4_1:42463552-42463571 Msa0548420:five_prime_UTR 30.0%
TTGAAAGATTAGTGGGAACT+AGG + chr4_1:42460256-42460275 None:intergenic 35.0%
TAGTTCTTGTTCCTAGTACT+TGG - chr4_1:42460341-42460360 Msa0548420:three_prime_UTR 35.0%
!! ATAACGGATCCTTTAGATGA+AGG - chr4_1:42460511-42460530 Msa0548420:three_prime_UTR 35.0%
!!! TCATAGTGGATTAGGGTTTT+CGG - chr4_1:42460969-42460988 Msa0548420:CDS 35.0%
! CATTTTTCTTTCCTCGATCT+TGG + chr4_1:42461000-42461019 None:intergenic 35.0%
CAAGATCGAGGAAAGAAAAA+TGG - chr4_1:42460998-42461017 Msa0548420:CDS 35.0%
TCTATTGTTACTCCGATCAA+AGG - chr4_1:42461072-42461091 Msa0548420:intron 35.0%
ATTCTCATTATGCTTCTCAC+CGG + chr4_1:42461133-42461152 None:intergenic 35.0%
TTTGAGTAGTGAAGGTAGTT+TGG - chr4_1:42461173-42461192 Msa0548420:intron 35.0%
TTTGGAAGATGATGATTCGT+TGG - chr4_1:42461191-42461210 Msa0548420:intron 35.0%
TTGGAAGATGATGATTCGTT+GGG - chr4_1:42461192-42461211 Msa0548420:intron 35.0%
!! TGTTCATGTTGTTGTTTCTG+AGG - chr4_1:42461260-42461279 Msa0548420:intron 35.0%
! AACATGGTTGGGTTTTTGTT+GGG - chr4_1:42461283-42461302 Msa0548420:intron 35.0%
AACAAATAATCAGGTGCATC+AGG + chr4_1:42461330-42461349 None:intergenic 35.0%
!! GTTGATGATGATGTTGTTGA+TGG - chr4_1:42461447-42461466 Msa0548420:intron 35.0%
! CAATGTGTTGATCAAGAGAA+AGG - chr4_1:42461510-42461529 Msa0548420:intron 35.0%
AGAGCTTGATAGAAGATTGA+AGG - chr4_1:42461653-42461672 Msa0548420:CDS 35.0%
AGATTGAAGGAGCAATTAAG+TGG - chr4_1:42461666-42461685 Msa0548420:CDS 35.0%
AACATCAAGATAAGACTCCT+CGG + chr4_1:42461763-42461782 None:intergenic 35.0%
! AATCCGGAACTTGCTTTAAT+GGG - chr4_1:42461804-42461823 Msa0548420:intron 35.0%
! TTGGATTGGGAAAATTAGAC+AGG - chr4_1:42461926-42461945 Msa0548420:CDS 35.0%
TGGGAAAATTAGACAGGATT+TGG - chr4_1:42461932-42461951 Msa0548420:CDS 35.0%
AAATTAGACAGGATTTGGAG+AGG - chr4_1:42461937-42461956 Msa0548420:CDS 35.0%
CAATGCATGATCTTGAATCA+TGG - chr4_1:42461973-42461992 Msa0548420:CDS 35.0%
!! AGCTGAAAAGCACTTAAACT+AGG + chr4_1:42462026-42462045 None:intergenic 35.0%
AACATTGGTACTATGATCCT+TGG + chr4_1:42462051-42462070 None:intergenic 35.0%
TCATAGTACCAATGTTGAAG+AGG - chr4_1:42462055-42462074 Msa0548420:CDS 35.0%
CCAAAACAGAAGTCTAAAAG+AGG + chr4_1:42462091-42462110 None:intergenic 35.0%
! CCTCTTTTAGACTTCTGTTT+TGG - chr4_1:42462088-42462107 Msa0548420:CDS 35.0%
! GGATGGATTTAGATTGCTTA+CGG - chr4_1:42462350-42462369 Msa0548420:CDS 35.0%
ATTTCAGGCATTCACAAAGT+CGG + chr4_1:42462403-42462422 None:intergenic 35.0%
! CTATCCTTTTGGATTTGGAT+TGG + chr4_1:42462437-42462456 None:intergenic 35.0%
CAATCCAAATCCAAAAGGAT+AGG - chr4_1:42462435-42462454 Msa0548420:CDS 35.0%
TGTGACAAGAGAAACAAATG+TGG + chr4_1:42462500-42462519 None:intergenic 35.0%
CACAAAGGAATACATATGCT+AGG - chr4_1:42462516-42462535 Msa0548420:CDS 35.0%
TATGGTTGGCAAGTAATGTA+TGG + chr4_1:42462882-42462901 None:intergenic 35.0%
! TGTGGTGTTTAAGATATGGT+TGG + chr4_1:42462896-42462915 None:intergenic 35.0%
!! TTTTTGATTCTATGCTCCGA+CGG - chr4_1:42462936-42462955 Msa0548420:CDS 35.0%
TTCATCACATTACAACCTGA+AGG - chr4_1:42463008-42463027 Msa0548420:CDS 35.0%
! TTTCTTTGGAAGGAAGGATA+TGG - chr4_1:42463285-42463304 Msa0548420:CDS 35.0%
ATGTAAATCAGTGGATAGTG+AGG - chr4_1:42463561-42463580 Msa0548420:five_prime_UTR 35.0%
GATTGTAAAGTTGTAAACCC+TGG - chr4_1:42463583-42463602 Msa0548420:five_prime_UTR 35.0%
!! ACGGTTACTGTTGTTGTTTC+CGG + chr4_1:42460470-42460489 None:intergenic 40.0%
GATCCTTTAGATGAAGGACT+TGG - chr4_1:42460517-42460536 Msa0548420:three_prime_UTR 40.0%
TGACCAAGTCCTTCATCTAA+AGG + chr4_1:42460523-42460542 None:intergenic 40.0%
TTGGAAGAAGATAAACGGGT+CGG + chr4_1:42460571-42460590 None:intergenic 40.0%
GGTTTCTTCAGAGTGAACTT+TGG + chr4_1:42460590-42460609 None:intergenic 40.0%
ACAACTTTCCGAACAATCTC+CGG - chr4_1:42460610-42460629 Msa0548420:CDS 40.0%
AGCTCTATCAAAACAACCGT+AGG + chr4_1:42460695-42460714 None:intergenic 40.0%
AAGTTACCGGAAAAAGCCTT+CGG + chr4_1:42460769-42460788 None:intergenic 40.0%
!! AAGGCTTTTTCCGGTAACTT+TGG - chr4_1:42460769-42460788 Msa0548420:intron 40.0%
!! AGCTTCTCTCATAGTGGATT+AGG - chr4_1:42460961-42460980 Msa0548420:CDS 40.0%
!! GCTTCTCTCATAGTGGATTA+GGG - chr4_1:42460962-42460981 Msa0548420:CDS 40.0%
TGGGAAGTCAGAATCTTCAA+TGG - chr4_1:42461211-42461230 Msa0548420:intron 40.0%
GGGAAGTCAGAATCTTCAAT+GGG - chr4_1:42461212-42461231 Msa0548420:intron 40.0%
! AGAATCTTCAATGGGCTCAA+GGG - chr4_1:42461220-42461239 Msa0548420:intron 40.0%
!! GTTGTTGTTTCTGAGGAACA+TGG - chr4_1:42461267-42461286 Msa0548420:intron 40.0%
!! TTGTTTCTGAGGAACATGGT+TGG - chr4_1:42461271-42461290 Msa0548420:intron 40.0%
! TGTTTCTGAGGAACATGGTT+GGG - chr4_1:42461272-42461291 Msa0548420:intron 40.0%
! GAACATGGTTGGGTTTTTGT+TGG - chr4_1:42461282-42461301 Msa0548420:intron 40.0%
! GAATTTGAAACCGCGTGATT+CGG - chr4_1:42461425-42461444 Msa0548420:intron 40.0%
!! TGATGGTGTTGTTGTGAAAG+AGG - chr4_1:42461464-42461483 Msa0548420:intron 40.0%
! AAAGGAGATGGAAGTGTGAA+TGG - chr4_1:42461616-42461635 Msa0548420:intron 40.0%
! AAGGAGATGGAAGTGTGAAT+GGG - chr4_1:42461617-42461636 Msa0548420:intron 40.0%
! ATGGAAGTGTGAATGGGATA+GGG - chr4_1:42461623-42461642 Msa0548420:intron 40.0%
TTGAAGGAGCAATTAAGTGG+CGG - chr4_1:42461669-42461688 Msa0548420:CDS 40.0%
! GGAGTCTTATCTTGATGTTG+CGG - chr4_1:42461764-42461783 Msa0548420:intron 40.0%
TCTTGATGTTGCGGATAAGA+TGG - chr4_1:42461773-42461792 Msa0548420:intron 40.0%
!! TAAGATGGTGATGGAGAATC+CGG - chr4_1:42461788-42461807 Msa0548420:intron 40.0%
! GAATCCGGAACTTGCTTTAA+TGG - chr4_1:42461803-42461822 Msa0548420:intron 40.0%
GAACCCATTAAAGCAAGTTC+CGG + chr4_1:42461810-42461829 None:intergenic 40.0%
AGAAAGCCGAACCTGATTAT+TGG - chr4_1:42461907-42461926 Msa0548420:CDS 40.0%
CCCAATCCAATAATCAGGTT+CGG + chr4_1:42461916-42461935 None:intergenic 40.0%
CCGAACCTGATTATTGGATT+GGG - chr4_1:42461913-42461932 Msa0548420:CDS 40.0%
GGATTTGGAGAGGATTAATG+AGG - chr4_1:42461947-42461966 Msa0548420:CDS 40.0%
! AAGTGCTTTTCAGCTTACCA+AGG - chr4_1:42462031-42462050 Msa0548420:CDS 40.0%
TTTAACACGATCGTTGACCA+CGG + chr4_1:42462209-42462228 None:intergenic 40.0%
TCGTGTTAAAGGATCTCTCA+AGG - chr4_1:42462219-42462238 Msa0548420:CDS 40.0%
ACGAACCTGTTTGAGAAATC+CGG + chr4_1:42462263-42462282 None:intergenic 40.0%
TAAGAAACTCAACGGAAGGA+TGG - chr4_1:42462333-42462352 Msa0548420:CDS 40.0%
!!! TTTTGGATTTGGATTGGCTG+CGG + chr4_1:42462431-42462450 None:intergenic 40.0%
! AGAAGTGCATTGTTCCACTT+GGG + chr4_1:42462825-42462844 None:intergenic 40.0%
!! TGTCTTTTTGGCCTAATCTG+TGG + chr4_1:42462914-42462933 None:intergenic 40.0%
!! CAAGTATTGATAGAGTCCGT+CGG + chr4_1:42462955-42462974 None:intergenic 40.0%
GAATTGCTCGAAATTCCACA+AGG - chr4_1:42463221-42463240 Msa0548420:CDS 40.0%
TCGAAATTCCACAAGGTGAT+AGG - chr4_1:42463228-42463247 Msa0548420:CDS 40.0%
AAATTCCACAAGGTGATAGG+CGG - chr4_1:42463231-42463250 Msa0548420:CDS 40.0%
TATGAGATATATGCTGCCGA+AGG - chr4_1:42463378-42463397 Msa0548420:CDS 40.0%
ATGAGATATATGCTGCCGAA+GGG - chr4_1:42463379-42463398 Msa0548420:CDS 40.0%
TGGAAGAAGATAAACGGGTC+GGG + chr4_1:42460570-42460589 None:intergenic 45.0%
GGAGATTGTTCGGAAAGTTG+TGG + chr4_1:42460611-42460630 None:intergenic 45.0%
!! GCTTTTTCCGGTAACTTTGG+TGG - chr4_1:42460772-42460791 Msa0548420:intron 45.0%
ACCAACACCACCAAAGTTAC+CGG + chr4_1:42460782-42460801 None:intergenic 45.0%
GACATAAACCCACGCTCTAA+CGG + chr4_1:42460835-42460854 None:intergenic 45.0%
TTAGAGCGTGGGTTTATGTC+CGG - chr4_1:42460835-42460854 Msa0548420:CDS 45.0%
TAAACCACGATCAATCGGAC+CGG + chr4_1:42460884-42460903 None:intergenic 45.0%
!! TGGTTTATTTTCCGGTCCGA+TGG - chr4_1:42460897-42460916 Msa0548420:CDS 45.0%
GCAATCTTTATCCATCGGAC+CGG + chr4_1:42460911-42460930 None:intergenic 45.0%
CCGGAGCAATCTTTATCCAT+CGG + chr4_1:42460916-42460935 None:intergenic 45.0%
CCGATGGATAAAGATTGCTC+CGG - chr4_1:42460913-42460932 Msa0548420:CDS 45.0%
!! TCTCTGAAACTGATCAACGC+CGG + chr4_1:42460941-42460960 None:intergenic 45.0%
CAGAGAAGCTTCTCTCATAG+TGG - chr4_1:42460955-42460974 Msa0548420:CDS 45.0%
ATTTCGAAGACTCTGTCACG+TGG - chr4_1:42461042-42461061 Msa0548420:CDS 45.0%
TGTTACTCCGATCAAAGGTG+TGG - chr4_1:42461077-42461096 Msa0548420:intron 45.0%
! CAGAATCTTCAATGGGCTCA+AGG - chr4_1:42461219-42461238 Msa0548420:intron 45.0%
AGGAGCTTAAAGGGTTGCTT+TGG - chr4_1:42461376-42461395 Msa0548420:intron 45.0%
! GGAGCTTAAAGGGTTGCTTT+GGG - chr4_1:42461377-42461396 Msa0548420:intron 45.0%
!! AAAGGGTTGCTTTGGGATGA+TGG - chr4_1:42461384-42461403 Msa0548420:intron 45.0%
!! AAGGGTTGCTTTGGGATGAT+GGG - chr4_1:42461385-42461404 Msa0548420:intron 45.0%
!! TTGGGATGATGGGGTTTCAT+CGG - chr4_1:42461395-42461414 Msa0548420:intron 45.0%
TCATCATCAACCGAATCACG+CGG + chr4_1:42461438-42461457 None:intergenic 45.0%
! AGAAGCACGAGGAGAATCAA+AGG - chr4_1:42461598-42461617 Msa0548420:intron 45.0%
ACGAGGAGAATCAAAGGAGA+TGG - chr4_1:42461604-42461623 Msa0548420:intron 45.0%
! GATGGAAGTGTGAATGGGAT+AGG - chr4_1:42461622-42461641 Msa0548420:intron 45.0%
TGTTGCGGATAAGATGGTGA+TGG - chr4_1:42461779-42461798 Msa0548420:intron 45.0%
! GGGAGATAGTAGAGCTGTTT+TGG - chr4_1:42461881-42461900 Msa0548420:CDS 45.0%
GCCGAACCTGATTATTGGAT+TGG - chr4_1:42461912-42461931 Msa0548420:CDS 45.0%
GTGGTCAACGATCGTGTTAA+AGG - chr4_1:42462208-42462227 Msa0548420:CDS 45.0%
CTCAAGGTTACTCGAGCATT+TGG - chr4_1:42462235-42462254 Msa0548420:CDS 45.0%
AATCCCACACAGTTAACACG+AGG - chr4_1:42462377-42462396 Msa0548420:CDS 45.0%
GCAGCCAATCCAAATCCAAA+AGG - chr4_1:42462430-42462449 Msa0548420:CDS 45.0%
GTTATTGTTTGCAGCCCAAG+TGG - chr4_1:42462808-42462827 Msa0548420:CDS 45.0%
! CAGAAGTGCATTGTTCCACT+TGG + chr4_1:42462826-42462845 None:intergenic 45.0%
CAAGTGGAACAATGCACTTC+TGG - chr4_1:42462824-42462843 Msa0548420:CDS 45.0%
CTTCGACCAAATGTTGAGCA+GGG + chr4_1:42463037-42463056 None:intergenic 45.0%
TCTTCGACCAAATGTTGAGC+AGG + chr4_1:42463038-42463057 None:intergenic 45.0%
! CAATGATGGAAACATCGTCG+TGG + chr4_1:42463262-42463281 None:intergenic 45.0%
AAAAAGGATGCGTGACCCTT+CGG + chr4_1:42463397-42463416 None:intergenic 45.0%
AGAAGATAAACGGGTCGGGT+CGG + chr4_1:42460566-42460585 None:intergenic 50.0%
CCGGAAAAAGCCTTCGGAAT+CGG + chr4_1:42460763-42460782 None:intergenic 50.0%
!! CCGATTCCGAAGGCTTTTTC+CGG - chr4_1:42460760-42460779 Msa0548420:intron 50.0%
!! TCCGGTAACTTTGGTGGTGT+TGG - chr4_1:42460778-42460797 Msa0548420:intron 50.0%
!! GGTAACTTTGGTGGTGTTGG+TGG - chr4_1:42460781-42460800 Msa0548420:intron 50.0%
!! AACTTTGGTGGTGTTGGTGG+TGG - chr4_1:42460784-42460803 Msa0548420:intron 50.0%
GACATGAACCCACGCTCAAT+CGG + chr4_1:42460862-42460881 None:intergenic 50.0%
ATTGAGCGTGGGTTCATGTC+CGG - chr4_1:42460862-42460881 Msa0548420:CDS 50.0%
GATAAAGATTGCTCCGGCAC+CGG - chr4_1:42460919-42460938 Msa0548420:CDS 50.0%
! TTTCGGTTCGACCAAGATCG+AGG - chr4_1:42460986-42461005 Msa0548420:CDS 50.0%
! TACTCCGATCAAAGGTGTGG+CGG - chr4_1:42461080-42461099 Msa0548420:intron 50.0%
TTCACCGCCACACCTTTGAT+CGG + chr4_1:42461087-42461106 None:intergenic 50.0%
! AATGGGCTCAAGGGAAAGCA+GGG - chr4_1:42461229-42461248 Msa0548420:intron 50.0%
!! AGGGTTGCTTTGGGATGATG+GGG - chr4_1:42461386-42461405 Msa0548420:intron 50.0%
ATTCTCCTCGTGCTTCTTCG+CGG + chr4_1:42461595-42461614 None:intergenic 50.0%
AAGCTCTTTCGCGAGCTTTG+AGG - chr4_1:42461733-42461752 Msa0548420:intron 50.0%
!! GTTACTCGAGCATTTGGTGC+CGG - chr4_1:42462241-42462260 Msa0548420:CDS 50.0%
! TGGTGCCGGATTTCTCAAAC+AGG - chr4_1:42462255-42462274 Msa0548420:CDS 50.0%
TTGGCTGCGGTCACAATTTC+AGG + chr4_1:42462418-42462437 None:intergenic 50.0%
AGGAGACCCTGCTCAACATT+TGG - chr4_1:42463028-42463047 Msa0548420:CDS 50.0%
TTTCGCGCTGCAAAGAAAGC+TGG - chr4_1:42463065-42463084 Msa0548420:CDS 50.0%
GCGAGGGGGTAAGAACAAAA+AGG + chr4_1:42463413-42463432 None:intergenic 50.0%
TCGACACTCGTGAATAGCGA+GGG + chr4_1:42463429-42463448 None:intergenic 50.0%
GAAGATAAACGGGTCGGGTC+GGG + chr4_1:42460565-42460584 None:intergenic 55.0%
!! CGTTGAAGTGTTGGCGCTGA+CGG + chr4_1:42460641-42460660 None:intergenic 55.0%
! CGACAACGGCGTTGAAGTGT+TGG + chr4_1:42460650-42460669 None:intergenic 55.0%
CACTTCAACGCCGTTGTCGA+CGG - chr4_1:42460651-42460670 Msa0548420:CDS 55.0%
TCCATGAACGCCGTCGACAA+CGG + chr4_1:42460664-42460683 None:intergenic 55.0%
! TCCGTTGCAACCGATTCCGA+AGG - chr4_1:42460750-42460769 Msa0548420:intron 55.0%
GCCTTCGGAATCGGTTGCAA+CGG + chr4_1:42460754-42460773 None:intergenic 55.0%
!! TTTGGTGGTGTTGGTGGTGG+TGG - chr4_1:42460787-42460806 Msa0548420:intron 55.0%
!! GGTGGTGGTTTTCCGAGTTC+CGG - chr4_1:42460808-42460827 Msa0548420:intron 55.0%
! AAACCCACGCTCTAACGGAC+CGG + chr4_1:42460830-42460849 None:intergenic 55.0%
ATGTCCGGTCCGATTGATCG+TGG - chr4_1:42460877-42460896 Msa0548420:CDS 55.0%
AAACTGATCAACGCCGGTGC+CGG + chr4_1:42460935-42460954 None:intergenic 55.0%
! GATCAAAGGTGTGGCGGTGA+AGG - chr4_1:42461086-42461105 Msa0548420:intron 55.0%
TCACCGGCAAGAATCCACTC+CGG + chr4_1:42461117-42461136 None:intergenic 55.0%
! CAATGGGCTCAAGGGAAAGC+AGG - chr4_1:42461228-42461247 Msa0548420:intron 55.0%
ATGGGCTCAAGGGAAAGCAG+GGG - chr4_1:42461230-42461249 Msa0548420:intron 55.0%
!! GCGAGCTTTGAGGAAAACCG+AGG - chr4_1:42461743-42461762 Msa0548420:intron 55.0%
ACCACGGCACAAGGATCATC+CGG + chr4_1:42462193-42462212 None:intergenic 55.0%
CGGCCTCGTGTTAACTGTGT+GGG + chr4_1:42462383-42462402 None:intergenic 55.0%
TCGGCCTCGTGTTAACTGTG+TGG + chr4_1:42462384-42462403 None:intergenic 55.0%
TGTTGAGCAGGGTCTCCTTC+AGG + chr4_1:42463026-42463045 None:intergenic 55.0%
TAACGCCGCCTATCACCTTG+TGG + chr4_1:42463239-42463258 None:intergenic 55.0%
GACACTCGTGAATAGCGAGG+GGG + chr4_1:42463427-42463446 None:intergenic 55.0%
CGACACTCGTGAATAGCGAG+GGG + chr4_1:42463428-42463447 None:intergenic 55.0%
CTCGACACTCGTGAATAGCG+AGG + chr4_1:42463430-42463449 None:intergenic 55.0%
!! TGAAGTGTTGGCGCTGACGG+AGG + chr4_1:42460638-42460657 None:intergenic 60.0%
! CGCTCTAACGGACCGGAACT+CGG + chr4_1:42460823-42460842 None:intergenic 60.0%
AGTTCCGGTCCGTTAGAGCG+TGG - chr4_1:42460823-42460842 Msa0548420:CDS 60.0%
GTTCCGGTCCGTTAGAGCGT+GGG - chr4_1:42460824-42460843 Msa0548420:CDS 60.0%
ATGTCCGGTCCGATTGAGCG+TGG - chr4_1:42460850-42460869 Msa0548420:CDS 60.0%
TGTCCGGTCCGATTGAGCGT+GGG - chr4_1:42460851-42460870 Msa0548420:CDS 60.0%
GAACCCACGCTCAATCGGAC+CGG + chr4_1:42460857-42460876 None:intergenic 60.0%
! CCACCGGAGTGGATTCTTGC+CGG - chr4_1:42461111-42461130 Msa0548420:intron 60.0%
! AGATGCCGCGAAGAAGCACG+AGG - chr4_1:42461587-42461606 Msa0548420:intron 60.0%
CCACGGCACAAGGATCATCC+GGG + chr4_1:42462192-42462211 None:intergenic 60.0%
CGATCGTTGACCACGGCACA+AGG + chr4_1:42462202-42462221 None:intergenic 60.0%
!! CGGAGGCGCCGGAGATTGTT+CGG + chr4_1:42460621-42460640 None:intergenic 65.0%
GCCGTTGTCGACGGCGTTCA+TGG - chr4_1:42460660-42460679 Msa0548420:CDS 65.0%
!! GGTGGTGTTGGTGGTGGTGG+TGG - chr4_1:42460790-42460809 Msa0548420:intron 65.0%
!! GGTGTTGGTGGTGGTGGTGG+TGG - chr4_1:42460793-42460812 Msa0548420:intron 65.0%
TGTGGCGGTGAAGGAGCCAC+CGG - chr4_1:42461095-42461114 Msa0548420:intron 65.0%
CCGGCAAGAATCCACTCCGG+TGG + chr4_1:42461114-42461133 None:intergenic 65.0%
CCCGGATGATCCTTGTGCCG+TGG - chr4_1:42462189-42462208 Msa0548420:CDS 65.0%
CGGTGAAGGAGCCACCGGAG+TGG - chr4_1:42461100-42461119 Msa0548420:intron 70.0%
!! GTTGGCGCTGACGGAGGCGC+CGG + chr4_1:42460632-42460651 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 42460219 42463687 42460219 ID=Msa0548420;Name=Msa0548420
chr4_1 mRNA 42460219 42463687 42460219 ID=Msa0548420-mRNA-1;Parent=Msa0548420;Name=Msa0548420-mRNA-1;_AED=0.03;_eAED=0.03;_QI=211|1|1|1|1|1|4|365|713
chr4_1 exon 42460219 42460699 42460219 ID=Msa0548420-mRNA-1:exon:10925;Parent=Msa0548420-mRNA-1
chr4_1 exon 42460820 42461084 42460820 ID=Msa0548420-mRNA-1:exon:10924;Parent=Msa0548420-mRNA-1
chr4_1 exon 42461630 42461743 42461630 ID=Msa0548420-mRNA-1:exon:10923;Parent=Msa0548420-mRNA-1
chr4_1 exon 42461830 42463687 42461830 ID=Msa0548420-mRNA-1:exon:10922;Parent=Msa0548420-mRNA-1
chr4_1 five_prime_UTR 42463477 42463687 42463477 ID=Msa0548420-mRNA-1:five_prime_utr;Parent=Msa0548420-mRNA-1
chr4_1 CDS 42461830 42463476 42461830 ID=Msa0548420-mRNA-1:cds;Parent=Msa0548420-mRNA-1
chr4_1 CDS 42461630 42461743 42461630 ID=Msa0548420-mRNA-1:cds;Parent=Msa0548420-mRNA-1
chr4_1 CDS 42460820 42461084 42460820 ID=Msa0548420-mRNA-1:cds;Parent=Msa0548420-mRNA-1
chr4_1 CDS 42460584 42460699 42460584 ID=Msa0548420-mRNA-1:cds;Parent=Msa0548420-mRNA-1
chr4_1 three_prime_UTR 42460219 42460583 42460219 ID=Msa0548420-mRNA-1:three_prime_utr;Parent=Msa0548420-mRNA-1
Gene Sequence

>Msa0548420

ATGGGAAACAAAATAGGAAAATTAACGGTATGTTTCACCGGAAACAACAACAGTAACCGTAAACACGACATATCCATATTAATAACGGATCCTTTAGATGAAGGACTTGGTCACTCTTTCTGTTACGTTAGACCCGACCCGACCCGTTTATCTTCTTCCAAAGTTCACTCTGAAGAAACCACAACTTTCCGAACAATCTCCGGCGCCTCCGTCAGCGCCAACACTTCAACGCCGTTGTCGACGGCGTTCATGGATTTATACTCCTACGGTTGTTTTGATAGAGCTGCAGCTTTTGAAAGTTCAACTTCTTTTGCTTCTCTTCCGTTGCAACCGATTCCGAAGGCTTTTTCCGGTAACTTTGGTGGTGTTGGTGGTGGTGGTGGTGGTTTTCCGAGTTCCGGTCCGTTAGAGCGTGGGTTTATGTCCGGTCCGATTGAGCGTGGGTTCATGTCCGGTCCGATTGATCGTGGTTTATTTTCCGGTCCGATGGATAAAGATTGCTCCGGCACCGGCGTTGATCAGTTTCAGAGAAGCTTCTCTCATAGTGGATTAGGGTTTTCGGTTCGACCAAGATCGAGGAAAGAAAAATGGATTCGTGTTCTTCAACGAGCGATTTCGAAGACTCTGTCACGTGGACAGAATTCTATTGTTACTCCGATCAAAGGTGTGGCGGTGAAGGAGCCACCGGAGTGGATTCTTGCCGGTGAGAAGCATAATGAGAATTTGACTGTGAGTAGTTTGAATTTGAGTAGTGAAGGTAGTTTGGAAGATGATGATTCGTTGGGAAGTCAGAATCTTCAATGGGCTCAAGGGAAAGCAGGGGAAGATCGTGTTCATGTTGTTGTTTCTGAGGAACATGGTTGGGTTTTTGTTGGGATTTATGATGGTTTTAATGGTCCTGATGCACCTGATTATTTGTTGTCAAATCTTTATAGTTTTGTTCATAAGGAGCTTAAAGGGTTGCTTTGGGATGATGGGGTTTCATCGGATAATTCGAATTTGAAACCGCGTGATTCGGTTGATGATGATGTTGTTGATGGTGTTGTTGTGAAAGAGGATATGTTGTTTGATGATTGTTCTCAATGTGTTGATCAAGAGAAAGGTGATTCGATTTCAAAGAAAAAAAAGAGAGGTAAGAATTCGAAGAATAAGTACAAAGATGCCGCGAAGAAGCACGAGGAGAATCAAAGGAGATGGAAGTGTGAATGGGATAGGGAGAGATTAGAGCTTGATAGAAGATTGAAGGAGCAATTAAGTGGCGGTGATGCCGACGATAATTCTGTTAATCATTCAGATGTTTTGGAAGCTCTTTCGCGAGCTTTGAGGAAAACCGAGGAGTCTTATCTTGATGTTGCGGATAAGATGGTGATGGAGAATCCGGAACTTGCTTTAATGGGTTCATGTGTTCTTGTAATGTTGATGAAAGGGGAAGATGTTTATGTGATGAATGTGGGAGATAGTAGAGCTGTTTTGGCACAGAAAGCCGAACCTGATTATTGGATTGGGAAAATTAGACAGGATTTGGAGAGGATTAATGAGGAGACAATGCATGATCTTGAATCATGGGATGATGCTGATAAATCAAATGTTGTACCTAGTTTAAGTGCTTTTCAGCTTACCAAGGATCATAGTACCAATGTTGAAGAGGAAGTAATCAGAATAATAAAAGAACACCCGGATGATCCTTGTGCCGTGGTCAACGATCGTGTTAAAGGATCTCTCAAGGTTACTCGAGCATTTGGTGCCGGATTTCTCAAACAGCCCAAGTGGAACAATGCACTTCTGGAGATGTTTAGAATAGACTACGTAGGAAATTGTCCATACATTACTTGCCAACCATATCTTAAACACCACAGATTAGGCCAAAAAGACAAATTTTTGATTCTATGCTCCGACGGACTCTATCAATACTTGTCAAACGAAGAAGCAGTAGCTGAAGTTGAGCTTTTCATCACATTACAACCTGAAGGAGACCCTGCTCAACATTTGGTCGAAGAAGTCTTGTTTCGCGCTGCAAAGAAAGCTGGTTTGGACTTTCATGAATTGCTCGAAATTCCACAAGGTGATAGGCGGCGTTACCACGACGATGTTTCCATCATTGTTATTTCTTTGGAAGGAAGGATATGGAGATCATGTGTATAA
Protein sequence

>Msa0548420

MGNKIGKLTVCFTGNNNSNRKHDISILITDPLDEGLGHSFCYVRPDPTRLSSSKVHSEETTTFRTISGASVSANTSTPLSTAFMDLYSYGCFDRAAAFESSTSFASLPLQPIPKAFSGNFGGVGGGGGGFPSSGPLERGFMSGPIERGFMSGPIDRGLFSGPMDKDCSGTGVDQFQRSFSHSGLGFSVRPRSRKEKWIRVLQRAISKTLSRGQNSIVTPIKGVAVKEPPEWILAGEKHNENLTVSSLNLSSEGSLEDDDSLGSQNLQWAQGKAGEDRVHVVVSEEHGWVFVGIYDGFNGPDAPDYLLSNLYSFVHKELKGLLWDDGVSSDNSNLKPRDSVDDDVVDGVVVKEDMLFDDCSQCVDQEKGDSISKKKKRGKNSKNKYKDAAKKHEENQRRWKCEWDRERLELDRRLKEQLSGGDADDNSVNHSDVLEALSRALRKTEESYLDVADKMVMENPELALMGSCVLVMLMKGEDVYVMNVGDSRAVLAQKAEPDYWIGKIRQDLERINEETMHDLESWDDADKSNVVPSLSAFQLTKDHSTNVEEEVIRIIKEHPDDPCAVVNDRVKGSLKVTRAFGAGFLKQPKWNNALLEMFRIDYVGNCPYITCQPYLKHHRLGQKDKFLILCSDGLYQYLSNEEAVAEVELFITLQPEGDPAQHLVEEVLFRAAKKAGLDFHELLEIPQGDRRRYHDDVSIIVISLEGRIWRSCV*