AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0623080

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0623080 MtrunA17_Chr4g0030551 96.223 556 11 1 1 556 1 546 0.0 1083
Msa0623080 MtrunA17_Chr2g0327711 77.049 549 111 4 12 556 7 544 0.0 824
Msa0623080 MtrunA17_Chr7g0263421 58.692 535 206 4 22 556 1 520 0.0 598
Msa0623080 MtrunA17_Chr7g0276681 55.926 540 215 8 17 556 30 546 0.0 555
Msa0623080 MtrunA17_Chr1g0212971 45.745 564 255 10 16 556 58 593 2.56e-151 447
Msa0623080 MtrunA17_Chr1g0161231 42.751 538 272 9 35 556 14 531 3.67e-141 418
Msa0623080 MtrunA17_Chr8g0391711 44.112 501 249 8 66 556 59 538 6.75e-135 402
Msa0623080 MtrunA17_Chr7g0263471 42.832 558 268 10 19 556 56 582 8.12e-126 381
Msa0623080 MtrunA17_Chr2g0331191 26.003 573 315 17 28 524 67 606 2.07e-37 146
Msa0623080 MtrunA17_Chr2g0331191 25.188 266 182 5 32 296 2 251 3.42e-15 78.6
Msa0623080 MtrunA17_Chr2g0331201 25.400 563 310 17 32 517 71 600 2.95e-32 131
Msa0623080 MtrunA17_Chr2g0331201 24.706 255 150 7 72 298 38 278 1.42e-16 83.2
Msa0623080 MtrunA17_Chr5g0439161 26.004 473 284 16 67 501 207 651 9.63e-18 87.0
Msa0623080 MtrunA17_Chr5g0439361 22.955 379 256 8 133 490 5 368 9.67e-16 79.7
Msa0623080 MtrunA17_Chr7g0262731 28.897 263 150 9 74 315 9 255 1.13e-14 77.0
Msa0623080 MtrunA17_Chr7g0262731 28.505 214 141 6 147 351 39 249 5.14e-13 71.6
Msa0623080 MtrunA17_Chr8g0376971 40.367 109 63 1 152 258 188 296 1.46e-14 76.6
Msa0623080 MtrunA17_Chr8g0376971 25.822 213 134 3 108 304 188 392 1.73e-12 70.1
Msa0623080 MtrunA17_Chr8g0376971 26.540 211 122 7 103 292 98 296 1.53e-11 67.0
Msa0623080 MtrunA17_Chr8g0370361 24.497 298 179 7 109 394 47 310 1.71e-14 75.9
Msa0623080 MtrunA17_Chr3g0122941 32.683 205 126 5 58 258 94 290 1.78e-14 76.6
Msa0623080 MtrunA17_Chr3g0122941 25.850 294 177 11 35 304 109 385 7.52e-14 74.7
Msa0623080 MtrunA17_Chr3g0122941 26.829 205 124 4 100 292 100 290 9.57e-12 67.8
Msa0623080 MtrunA17_Chr5g0418551 33.750 160 101 4 153 310 58 214 2.52e-14 73.2
Msa0623080 MtrunA17_Chr6g0458951 31.308 214 118 6 108 304 192 393 2.68e-14 75.9
Msa0623080 MtrunA17_Chr8g0367671 23.762 303 183 6 108 396 47 315 3.77e-14 74.7
Msa0623080 MtrunA17_Chr8g0361991 24.207 347 204 9 74 396 11 322 1.71e-13 72.8
Msa0623080 MtrunA17_Chr3g0136651 22.717 449 237 15 107 487 3 409 3.90e-13 71.6
Msa0623080 MtrunA17_Chr8g0364031 24.906 265 173 4 74 325 10 261 4.18e-13 71.6
Msa0623080 MtrunA17_Chr1g0211241 28.500 200 130 4 109 308 139 325 5.28e-13 71.6
Msa0623080 MtrunA17_Chr8g0366431 25.175 286 157 8 183 440 32 288 2.57e-12 68.6
Msa0623080 MtrunA17_Chr5g0439191 33.766 154 93 5 145 290 1 153 2.86e-12 65.5
Msa0623080 MtrunA17_Chr2g0293861 32.680 153 94 5 146 290 27 178 4.76e-12 65.5
Msa0623080 MtrunA17_Chr5g0439351 20.972 391 260 8 129 490 315 685 5.02e-12 68.9
Msa0623080 MtrunA17_Chr8g0354071 22.687 335 209 8 134 442 10 320 6.72e-12 67.4
Msa0623080 MtrunA17_Chr1g0178581 24.926 337 171 10 106 394 9 311 1.63e-11 66.6
Msa0623080 MtrunA17_Chr3g0093531 22.687 335 209 8 134 442 10 320 2.41e-11 65.9
Msa0623080 MtrunA17_Chr8g0353311 23.793 290 154 6 151 394 48 316 3.25e-11 65.5
Msa0623080 MtrunA17_Chr2g0328031 26.818 220 126 5 71 271 12 215 9.00e-11 64.3
Msa0623080 MtrunA17_Chr3g0083331 22.086 326 209 8 104 394 5 320 9.38e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0623080 AT1G07710.2 68.393 560 156 6 1 556 1 543 0.0 715
Msa0623080 AT1G07710.1 68.393 560 156 6 1 556 1 543 0.0 715
Msa0623080 AT5G60070.1 63.604 555 183 6 7 556 8 548 0.0 677
Msa0623080 AT5G02620.4 59.963 542 191 5 19 556 5 524 0.0 625
Msa0623080 AT5G02620.1 59.963 542 191 5 19 556 5 524 0.0 625
Msa0623080 AT5G02620.3 59.963 542 191 5 19 556 5 524 0.0 625
Msa0623080 AT5G02620.5 57.699 565 190 6 19 556 5 547 0.0 613
Msa0623080 AT5G02620.2 57.699 565 190 6 19 556 5 547 0.0 613
Msa0623080 AT2G31820.1 54.792 553 226 9 8 556 130 662 0.0 558
Msa0623080 AT1G05640.1 55.797 552 212 11 15 556 98 627 0.0 546
Msa0623080 AT3G12360.1 45.242 557 264 10 16 556 59 590 3.39e-144 429
Msa0623080 AT3G12360.2 45.242 557 264 10 16 556 47 578 4.22e-144 428
Msa0623080 AT3G09550.1 43.548 558 269 10 19 556 76 607 2.94e-133 401
Msa0623080 AT3G09550.3 43.548 558 269 10 19 556 76 607 2.94e-133 401
Msa0623080 AT3G09550.2 43.548 558 269 10 19 556 83 614 3.50e-133 401
Msa0623080 AT2G01680.1 43.260 497 249 5 35 517 14 491 8.88e-132 395
Msa0623080 AT1G34050.1 26.326 509 318 12 21 517 75 538 1.42e-36 144
Msa0623080 AT2G24600.3 25.440 511 314 19 32 511 72 546 1.15e-35 141
Msa0623080 AT2G24600.4 25.440 511 314 19 32 511 72 546 1.15e-35 141
Msa0623080 AT5G54710.1 25.095 526 324 14 32 533 103 582 1.40e-33 135
Msa0623080 AT5G54710.2 25.095 526 324 14 32 533 148 627 1.79e-33 135
Msa0623080 AT5G54710.2 23.154 298 177 5 32 300 19 293 1.03e-12 71.2
Msa0623080 AT5G54710.2 27.143 210 122 2 151 333 20 225 4.62e-12 69.3
Msa0623080 AT1G10340.2 25.385 520 335 16 31 511 37 542 1.49e-31 129
Msa0623080 AT1G10340.1 25.047 527 332 17 31 511 37 546 1.86e-30 125
Msa0623080 AT4G03500.1 24.231 553 342 18 4 496 64 599 2.84e-28 120
Msa0623080 AT4G03500.2 24.231 553 342 18 4 496 41 576 3.33e-28 119
Msa0623080 AT4G03500.3 24.231 553 342 18 4 496 41 576 3.33e-28 119
Msa0623080 AT4G14390.1 26.606 436 267 11 33 438 168 580 3.98e-25 110
Msa0623080 AT4G14390.2 26.606 436 266 12 33 438 149 560 7.95e-25 109
Msa0623080 AT4G14390.4 26.606 436 266 12 33 438 168 579 8.35e-25 109
Msa0623080 AT1G03670.1 24.540 489 279 14 29 482 144 577 1.70e-24 108
Msa0623080 AT1G03670.1 24.847 326 202 9 4 304 33 340 1.01e-16 84.0
Msa0623080 AT4G03470.1 23.846 520 303 16 27 501 149 620 7.84e-24 106
Msa0623080 AT5G54700.2 26.706 337 175 10 175 496 23 302 1.93e-22 99.4
Msa0623080 AT5G50140.1 23.540 531 305 18 27 527 57 516 3.63e-22 100
Msa0623080 AT5G50140.1 23.929 280 179 7 80 341 6 269 3.72e-12 69.3
Msa0623080 AT4G03460.1 23.983 467 273 15 32 439 81 524 1.12e-21 99.4
Msa0623080 AT4G03460.1 33.824 136 79 2 144 268 75 210 6.63e-13 72.0
Msa0623080 AT4G03480.2 25.570 395 222 10 67 439 309 653 2.22e-21 98.6
Msa0623080 AT4G03480.1 25.570 395 222 10 67 439 312 656 2.60e-21 98.6
Msa0623080 AT2G24600.2 26.055 403 240 16 32 409 72 441 4.39e-21 97.4
Msa0623080 AT2G24600.1 26.055 403 240 16 32 409 72 441 4.39e-21 97.4
Msa0623080 AT4G03460.2 23.341 437 258 13 62 439 74 492 4.65e-21 97.4
Msa0623080 AT4G10720.2 26.012 346 202 10 109 437 41 349 1.19e-20 95.1
Msa0623080 AT4G10720.1 27.063 303 173 8 109 394 41 312 2.72e-20 94.4
Msa0623080 AT4G05040.3 24.943 437 280 11 30 439 148 563 4.79e-20 94.4
Msa0623080 AT4G05040.3 28.161 174 110 3 150 308 82 255 8.75e-11 65.1
Msa0623080 AT4G05040.2 24.943 437 280 11 30 439 148 563 4.79e-20 94.4
Msa0623080 AT4G05040.2 28.161 174 110 3 150 308 82 255 8.75e-11 65.1
Msa0623080 AT4G05040.5 24.943 437 280 11 30 439 148 563 4.79e-20 94.4
Msa0623080 AT4G05040.5 28.161 174 110 3 150 308 82 255 8.75e-11 65.1
Msa0623080 AT4G05040.4 24.943 437 280 11 30 439 148 563 4.79e-20 94.4
Msa0623080 AT4G05040.4 28.161 174 110 3 150 308 82 255 8.75e-11 65.1
Msa0623080 AT4G05040.1 24.943 437 280 11 30 439 148 563 4.79e-20 94.4
Msa0623080 AT4G05040.1 28.161 174 110 3 150 308 82 255 8.75e-11 65.1
Msa0623080 AT4G05040.6 24.943 437 280 11 30 439 148 563 4.79e-20 94.4
Msa0623080 AT4G05040.6 28.161 174 110 3 150 308 82 255 8.75e-11 65.1
Msa0623080 AT4G05040.7 24.943 437 280 11 30 439 148 563 4.79e-20 94.4
Msa0623080 AT4G05040.7 28.161 174 110 3 150 308 82 255 8.75e-11 65.1
Msa0623080 AT5G54700.1 24.927 341 197 10 180 496 34 339 4.29e-19 90.9
Msa0623080 AT5G51160.1 25.135 370 219 11 145 471 43 397 7.61e-19 89.7
Msa0623080 AT4G14390.3 26.087 391 241 11 33 393 149 521 3.32e-17 85.1
Msa0623080 AT4G14400.2 23.542 463 254 11 33 439 71 489 4.90e-17 85.1
Msa0623080 AT4G14400.3 23.542 463 254 11 33 439 71 489 4.90e-17 85.1
Msa0623080 AT4G14400.1 23.542 463 254 11 33 439 137 555 7.37e-17 84.3
Msa0623080 AT4G03490.2 20.898 512 285 15 62 510 64 518 2.51e-16 82.8
Msa0623080 AT4G03490.4 21.333 525 305 15 62 510 84 576 2.62e-16 82.8
Msa0623080 AT4G03490.3 21.333 525 305 15 62 510 84 576 3.07e-16 82.8
Msa0623080 AT4G03490.5 21.333 525 305 15 62 510 74 566 3.07e-16 82.4
Msa0623080 AT4G03490.1 20.898 512 285 15 62 510 74 528 4.51e-16 82.0
Msa0623080 AT4G03440.1 24.806 387 238 14 67 439 269 616 5.56e-15 78.6
Msa0623080 AT4G03440.1 23.826 298 187 9 30 307 158 435 3.94e-11 66.2
Msa0623080 AT4G03440.2 24.806 387 238 14 67 439 262 609 6.07e-15 78.6
Msa0623080 AT4G03440.2 23.826 298 187 9 30 307 151 428 4.50e-11 66.2
Msa0623080 AT4G03440.3 24.806 387 238 14 67 439 262 609 6.07e-15 78.6
Msa0623080 AT4G03440.3 23.826 298 187 9 30 307 151 428 4.50e-11 66.2
Msa0623080 AT5G15500.2 25.215 349 206 10 71 398 3 317 8.84e-15 77.4
Msa0623080 AT3G04140.1 25.532 329 196 12 35 326 106 422 1.29e-13 74.3
Msa0623080 AT3G01750.2 26.459 257 140 8 35 258 109 349 4.17e-13 72.8
Msa0623080 AT3G01750.1 26.459 257 140 8 35 258 109 349 4.17e-13 72.8
Msa0623080 AT2G03430.1 31.373 204 123 7 66 267 43 231 4.33e-13 69.7
Msa0623080 AT2G03430.1 33.503 197 108 8 64 255 8 186 2.99e-11 64.3
Msa0623080 AT4G03450.1 24.453 274 169 6 27 298 101 338 3.41e-11 66.6
Msa0623080 AT4G03450.2 24.453 274 169 6 27 298 101 338 3.54e-11 66.2
Msa0623080 AT5G54620.1 28.571 147 103 1 128 272 14 160 4.34e-11 65.5
Msa0623080 AT1G14500.1 23.673 245 158 5 109 336 41 273 8.56e-11 64.7

Find 127 sgRNAs with CRISPR-Local

Find 232 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAATTGGGGTAACTATTAT+TGG 0.156648 4_3:-242720 Msa0623080:CDS
AATAAACATGGTTGATAATA+AGG 0.174079 4_3:-244248 Msa0623080:CDS
CATTGGGTACTATGAGTTAT+TGG 0.186716 4_3:-242677 Msa0623080:CDS
TTCTTCCTTTGAGCCCTTAC+TGG 0.196107 4_3:+245462 None:intergenic
CTATGAGTTATTGGGTTATT+AGG 0.231803 4_3:-242668 Msa0623080:CDS
TCAACCATGTTTATTGTTGA+TGG 0.242519 4_3:+244256 None:intergenic
TACCTGAGTTCTTCCCTTCC+TGG 0.243726 4_3:+244201 None:intergenic
GCTGCTAAACAAGGGGATTT+AGG 0.260704 4_3:-245168 Msa0623080:intron
ACAAAAGGAGTTCATTAGTT+TGG 0.269564 4_3:-245537 Msa0623080:five_prime_UTR
TTTCCTCGAGGTATGTGTTT+TGG 0.279074 4_3:+242990 None:intergenic
ACCCAATGTTGTAGCCATTA+TGG 0.293380 4_3:+242692 None:intergenic
TGTAACGCGGACTGATAAAA+AGG 0.298877 4_3:-244350 Msa0623080:CDS
CTTTATGTTGCTGCTGAGTA+TGG 0.312082 4_3:-245282 Msa0623080:CDS
AGATATTGTAAAGATCTTAA+TGG 0.322743 4_3:-244593 Msa0623080:CDS
CAATTGGGGTAACTATTATT+GGG 0.329062 4_3:-242719 Msa0623080:CDS
ATGGTTATGTTGATATGGTT+AGG 0.342881 4_3:-245263 Msa0623080:CDS
TTGCATTCTGCTGCAAGAAA+TGG 0.353298 4_3:-244418 Msa0623080:CDS
AAACAAAGGTGGCTTGCAAT+TGG 0.357257 4_3:-242735 Msa0623080:CDS
AACAAAGGTGGCTTGCAATT+GGG 0.360089 4_3:-242734 Msa0623080:CDS
ATTGGGTACTATGAGTTATT+GGG 0.363533 4_3:-242676 Msa0623080:CDS
CAGATTATCAAGTTGATACT+TGG 0.370218 4_3:-243395 Msa0623080:CDS
TGAGTATGGTTATGTTGATA+TGG 0.383585 4_3:-245268 Msa0623080:CDS
TGGGAACATGGCTTCTTTGA+AGG 0.392889 4_3:-245379 Msa0623080:CDS
CAAACACGAAGAAGCGTTCA+AGG 0.397506 4_3:-243149 Msa0623080:CDS
GTTTGGAGAATTGTATTCAA+TGG 0.398213 4_3:-245520 Msa0623080:exon
TGCTGCAAGAAATGGACATT+TGG 0.400973 4_3:-244410 Msa0623080:CDS
TATTATTGGGACTACCATAA+TGG 0.409865 4_3:-242706 Msa0623080:CDS
TTCAGTTTCCATGTCTTACA+TGG 0.411173 4_3:-246475 None:intergenic
ACCATAATGGCTACAACATT+GGG 0.411626 4_3:-242693 Msa0623080:CDS
TTTCATCTCCCTCGCGGTTG+TGG 0.414269 4_3:-242895 Msa0623080:CDS
TACCATAATGGCTACAACAT+TGG 0.414943 4_3:-242694 Msa0623080:CDS
GGATCGTCCACGAATTGGCC+TGG 0.416257 4_3:+243011 None:intergenic
TCTTTGAAGGATACAGTTGA+TGG 0.424259 4_3:-245366 Msa0623080:CDS
GCTGGAATTAAAGCAAGAAA+TGG 0.432637 4_3:-245210 Msa0623080:CDS
TTACATTCGGCAGCAAGAGC+TGG 0.434837 4_3:-245399 Msa0623080:CDS
AGCCTCAGGCTGCACCAGTA+AGG 0.434924 4_3:-245476 Msa0623080:CDS
TATTATGATCTTGCTGATGC+TGG 0.442983 4_3:-245228 Msa0623080:CDS
ATAAACATGGTTGATAATAA+GGG 0.448264 4_3:-244247 Msa0623080:CDS
GCAGCCATCTTCACCGTCCC+AGG 0.449913 4_3:-243029 Msa0623080:CDS
TTTAGTGGAACACACAAGTA+AGG 0.450272 4_3:-245933 Msa0623080:intron
CGATGATACGCCGTTACATT+CGG 0.459070 4_3:-245412 Msa0623080:CDS
TGGTTATGTTGATATGGTTA+GGG 0.459988 4_3:-245262 Msa0623080:CDS
GGCGCTTCACATGGCTGTGA+AGG 0.462145 4_3:-244320 Msa0623080:CDS
TTAGACACAGCGGAGAAAAC+AGG 0.464410 4_3:-243323 Msa0623080:CDS
AGCTGAAACTGTGCAGCCTC+AGG 0.466637 4_3:-245490 Msa0623080:CDS
AGTATTCATCGTGTTTGATT+CGG 0.466809 4_3:-242925 Msa0623080:CDS
ATTGAAGTCATAAGAACAAA+AGG 0.469472 4_3:-245552 Msa0623080:five_prime_UTR
ACAAAGGTGGCTTGCAATTG+GGG 0.480789 4_3:-242733 Msa0623080:CDS
GCGATTCTTGAGAAGGAGCC+AGG 0.483357 4_3:-244376 Msa0623080:CDS
GAAAATCTTCAATGGGAAGT+AGG 0.483651 4_3:-242620 Msa0623080:CDS
AGGGAACTAATATATGATGT+TGG 0.483762 4_3:+245984 None:intergenic
GCCTCAGGCTGCACCAGTAA+GGG 0.485466 4_3:-245475 Msa0623080:CDS
GAGTATAAGAAAATCTTCAA+TGG 0.486162 4_3:-242628 Msa0623080:CDS
GCAACAATTGCTAGAAGTAA+TGG 0.488412 4_3:-244451 Msa0623080:CDS
GATCGTCCACGAATTGGCCT+GGG 0.491991 4_3:+243012 None:intergenic
AGTATAAGAAAATCTTCAAT+GGG 0.497524 4_3:-242627 Msa0623080:CDS
TCAGATATACATGCACCAAC+AGG 0.503945 4_3:+246296 None:intergenic
AAGCAAGTGTTGTGAAGTAG+TGG 0.505499 4_3:+246406 None:intergenic
TTAGGCATAGAATCAATTTG+AGG 0.507230 4_3:-242650 Msa0623080:CDS
CTTCATATTGCTGCTAAACA+AGG 0.509684 4_3:-245177 Msa0623080:CDS
TACATTCGGCAGCAAGAGCT+GGG 0.514491 4_3:-245398 Msa0623080:CDS
GTGAAATATCTATTAGAAGC+AGG 0.514806 4_3:-244484 Msa0623080:CDS
AAAATGACGAAACAATTGAC+GGG 0.518046 4_3:-245441 Msa0623080:CDS
CATCTCCCTCGCGGTTGTGG+TGG 0.520207 4_3:-242892 Msa0623080:CDS
CACCGTCCCAGGCCAATTCG+TGG 0.520703 4_3:-243018 Msa0623080:CDS
ATGCACCAACAGGAAACAAA+AGG 0.529042 4_3:+246306 None:intergenic
TTGGCCTGGGACGGTGAAGA+TGG 0.531539 4_3:+243025 None:intergenic
CGAAAGCAACCGTTGCTATG+AGG 0.535432 4_3:+243051 None:intergenic
CTTGGACAAAGTGAAACAAA+TGG 0.535956 4_3:-243377 Msa0623080:CDS
GCGCTTCACATGGCTGTGAA+GGG 0.537881 4_3:-244319 Msa0623080:CDS
TGCAAGAAATGGACATTTGG+AGG 0.543958 4_3:-244407 Msa0623080:CDS
CAAAGCAAAGAAGCAAATGA+TGG 0.544079 4_3:-242841 Msa0623080:CDS
TTTGACGAGTGTGTTCTAGT+TGG 0.546084 4_3:+243168 None:intergenic
TCAGTCCGCGTTACAACACC+TGG 0.546749 4_3:+244358 None:intergenic
GCCCTTACTGGTGCAGCCTG+AGG 0.548134 4_3:+245474 None:intergenic
TACAGTTGATGGTGCTGAAG+AGG 0.548363 4_3:-245355 Msa0623080:CDS
TTGTGTTGCAGCAGTGTGCA+AGG 0.551289 4_3:+244522 None:intergenic
GGCAGCAAGAGCTGGGAACA+TGG 0.556799 4_3:-245391 Msa0623080:CDS
CGCTTCACATGGCTGTGAAG+GGG 0.560548 4_3:-244318 Msa0623080:CDS
GAAAATGACGAAACAATTGA+CGG 0.563779 4_3:-245442 Msa0623080:CDS
TGTGAAAGCGATTCTTGAGA+AGG 0.565310 4_3:-244383 Msa0623080:CDS
CTAATATATGATGTTGGTGT+AGG 0.567365 4_3:+245990 None:intergenic
GCAGAGTCTTGTGGTAGTAG+AGG 0.567525 4_3:-244296 Msa0623080:CDS
AGCAAGTGTTGTGAAGTAGT+GGG 0.567960 4_3:+246407 None:intergenic
ATTAGAAGCAGGTAGTAGCT+TGG 0.570550 4_3:-244473 Msa0623080:CDS
CTGCACCAGTAAGGGCTCAA+AGG 0.574302 4_3:-245467 Msa0623080:CDS
GAGGACCGCGACGACTGTTG+TGG 0.574532 4_3:+243070 None:intergenic
TTCATATTGCTGCTAAACAA+GGG 0.576566 4_3:-245176 Msa0623080:CDS
ACAGTTGATGGTGCTGAAGA+GGG 0.580772 4_3:-245354 Msa0623080:CDS
TGCAAGGCTGTTGTGTTGGA+CGG 0.580949 4_3:+244538 None:intergenic
ATATTTGCAAAGCAGAATCA+AGG 0.582207 4_3:-245318 Msa0623080:CDS
AACCAGGAAGGGAAGAACTC+AGG 0.589165 4_3:-244203 Msa0623080:intron
CTCAACAAAATGCACACAGA+AGG 0.589714 4_3:-243113 Msa0623080:CDS
TTGCACACTGCTGCAACACA+AGG 0.589896 4_3:-244520 Msa0623080:CDS
TCAGTTTCCATGTCTTACAT+GGG 0.591587 4_3:-246474 None:intergenic
GGCATGGCAGTAAACAAAAG+TGG 0.596158 4_3:-243356 Msa0623080:CDS
GCTCTTGCTGCCGAATGTAA+CGG 0.602344 4_3:+245402 None:intergenic
GGGACAGACGGCGCTTCACA+TGG 0.602708 4_3:-244329 Msa0623080:CDS
CTTCAATGGGAAGTAGGTCA+AGG 0.604224 4_3:-242614 Msa0623080:CDS
ATACCTCGAGGAAAATCCCT+TGG 0.605699 4_3:-242981 Msa0623080:CDS
AATTGCACAATGGAAAAGAG+TGG 0.606592 4_3:+242482 None:intergenic
GAGTGTGTTCTAGTTGGTGA+TGG 0.607313 4_3:+243174 None:intergenic
TCATATTGCTGCTAAACAAG+GGG 0.612141 4_3:-245175 Msa0623080:CDS
TTGCATATAGCAACCAGGAA+GGG 0.612446 4_3:-244214 Msa0623080:CDS
CAGCGTTGCATATAGCAACC+AGG 0.616173 4_3:-244219 Msa0623080:CDS
TGTGAAGGGGCAGAGTCTTG+TGG 0.617503 4_3:-244305 Msa0623080:CDS
TTCTGAATTGTCAATGACTG+TGG 0.617789 4_3:-244563 Msa0623080:CDS
CACGATGAATACTAGAAACG+CGG 0.624298 4_3:+242935 None:intergenic
GCAAGTGTTGTGAAGTAGTG+GGG 0.625343 4_3:+246408 None:intergenic
GTTGCATATAGCAACCAGGA+AGG 0.628632 4_3:-244215 Msa0623080:CDS
ACAAAGTGAAACAAATGGCA+TGG 0.631554 4_3:-243372 Msa0623080:CDS
GTCCACGAATTGGCCTGGGA+CGG 0.639953 4_3:+243016 None:intergenic
AGTGTGCAAGGCTGTTGTGT+TGG 0.641164 4_3:+244534 None:intergenic
CTATTATTAACAAACTAATG+TGG 0.644964 4_3:-242818 Msa0623080:CDS
CAAGTGTTGTGAAGTAGTGG+GGG 0.647416 4_3:+246409 None:intergenic
AAAAGCATTCTTACAGAACA+CGG 0.650500 4_3:-243287 Msa0623080:CDS
CGTCGCGGTCCTCATAGCAA+CGG 0.655824 4_3:-243060 Msa0623080:CDS
AGATCCATCAACAATAAACA+TGG 0.658313 4_3:-244260 Msa0623080:CDS
AGGAGCCAGGTGTTGTAACG+CGG 0.665895 4_3:-244363 Msa0623080:CDS
TTGAACCACCACAACCGCGA+GGG 0.673202 4_3:+242887 None:intergenic
ACGATGAATACTAGAAACGC+GGG 0.673783 4_3:+242936 None:intergenic
TTTGCAAAGCAGAATCAAGG+TGG 0.681219 4_3:-245315 Msa0623080:CDS
AAACTCCACAACAGTCGTCG+CGG 0.698686 4_3:-243075 Msa0623080:CDS
TTTGAACCACCACAACCGCG+AGG 0.726688 4_3:+242886 None:intergenic
AAATGACGAAACAATTGACG+GGG 0.731993 4_3:-245440 Msa0623080:CDS
GATCCAAAACACATACCTCG+AGG 0.745710 4_3:-242993 Msa0623080:CDS
CGAAACAGCATTAGACACAG+CGG 0.776505 4_3:-243333 Msa0623080:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAAAAATAAAATAAAAAA+TGG + chr4_3:243163-243182 None:intergenic 0.0%
!!! TCTAAATATTTTTACTATAT+TGG - chr4_3:242835-242854 Msa0623080:CDS 10.0%
!! AGTAAAAATATTTAGAAGAA+AGG + chr4_3:242831-242850 None:intergenic 15.0%
!! AAGAAAAAAAAAATCAAAGA+TGG + chr4_3:243111-243130 None:intergenic 15.0%
!! TTAATTCAATCCTAAAAAAT+TGG - chr4_3:245093-245112 Msa0623080:intron 15.0%
!!! TAAAAAAGCATTGAGATTTA+AGG + chr4_3:242997-243016 None:intergenic 20.0%
!! TCAGTGAAAAAATAAATAAG+AGG + chr4_3:243056-243075 None:intergenic 20.0%
!! TTATTATGATGAATGCTTAA+AGG - chr4_3:243077-243096 Msa0623080:CDS 20.0%
!!! TTTTGTGTTTTTGTTTTTGT+TGG - chr4_3:243335-243354 Msa0623080:CDS 20.0%
!!! AATCTCGATATTTTAAATTG+CGG - chr4_3:243873-243892 Msa0623080:intron 20.0%
!!! CATTTCTATTATTCAAGTTT+AGG + chr4_3:244215-244234 None:intergenic 20.0%
!!! TATCTATTTTATTTTGAACC+AGG - chr4_3:244303-244322 Msa0623080:CDS 20.0%
!!! ATCTATTTTATTTTGAACCA+GGG - chr4_3:244304-244323 Msa0623080:CDS 20.0%
!! AGATATTGTAAAGATCTTAA+TGG - chr4_3:244353-244372 Msa0623080:CDS 20.0%
!!! AATAAACATGGTTGATAATA+AGG - chr4_3:244698-244717 Msa0623080:intron 20.0%
!!! ATAAACATGGTTGATAATAA+GGG - chr4_3:244699-244718 Msa0623080:intron 20.0%
!!! ACGTTATAAACCAATTTTTT+AGG + chr4_3:245106-245125 None:intergenic 20.0%
!! ATACAATTCAATTATGCATA+AGG + chr4_3:245456-245475 None:intergenic 20.0%
!! CTATTATTAACAAACTAATG+TGG - chr4_3:246128-246147 Msa0623080:intron 20.0%
!! AGTATAAGAAAATCTTCAAT+GGG - chr4_3:246319-246338 Msa0623080:five_prime_UTR 20.0%
! TATGAAAAAACAAGATGAGA+GGG + chr4_3:242499-242518 None:intergenic 25.0%
! TTATGAAAAAACAAGATGAG+AGG + chr4_3:242500-242519 None:intergenic 25.0%
! AAGAAGAAAAAGAACAAAAG+AGG + chr4_3:242800-242819 None:intergenic 25.0%
!! AGATTTAAGGATAGCTTTTA+GGG + chr4_3:242984-243003 None:intergenic 25.0%
!!! AAATCTCAATGCTTTTTTAG+TGG - chr4_3:242998-243017 Msa0623080:CDS 25.0%
!! TTTTATCAGAGCAATGATTT+TGG - chr4_3:243177-243196 Msa0623080:CDS 25.0%
!!! ATCTTCTTTGATTTGTTTCA+AGG - chr4_3:243274-243293 Msa0623080:CDS 25.0%
! ATTGAAGTCATAAGAACAAA+AGG - chr4_3:243394-243413 Msa0623080:CDS 25.0%
! AAAATTCTATGACTGCAATT+TGG + chr4_3:244005-244024 None:intergenic 25.0%
!!! TTTTAAAAGAAAACCTTGAG+TGG - chr4_3:244023-244042 Msa0623080:intron 25.0%
! CAACTTGTGTTTATTTACTA+CGG + chr4_3:244181-244200 None:intergenic 25.0%
! AATAATAGAAATGTGTTGCA+AGG - chr4_3:244222-244241 Msa0623080:CDS 25.0%
! AACAAGATTTGATCAATGTT+CGG + chr4_3:244257-244276 None:intergenic 25.0%
! AACATTGATCAAATCTTGTT+TGG - chr4_3:244257-244276 Msa0623080:CDS 25.0%
! CTTTGAACAAAGATAAAGAA+GGG + chr4_3:244958-244977 None:intergenic 25.0%
! ACTTTGAACAAAGATAAAGA+AGG + chr4_3:244959-244978 None:intergenic 25.0%
! TATGTGAATAATGTGTTAGA+TGG - chr4_3:245028-245047 Msa0623080:intron 25.0%
! AATCTGAACAGAGTTTATAA+AGG + chr4_3:245138-245157 None:intergenic 25.0%
! ACTGTTAATGTAAAGAACAT+AGG - chr4_3:245260-245279 Msa0623080:CDS 25.0%
! TTGAATTGTATCTACCTATT+TGG - chr4_3:245465-245484 Msa0623080:CDS 25.0%
!! AATTAACCTCTATCAACTTT+TGG - chr4_3:245523-245542 Msa0623080:exon 25.0%
! GAGTATAAGAAAATCTTCAA+TGG - chr4_3:246318-246337 Msa0623080:five_prime_UTR 25.0%
!!! ACGGTCTTTTTTTTTGTTAC+AGG - chr4_3:242929-242948 Msa0623080:CDS 30.0%
!!! CGGTCTTTTTTTTTGTTACA+GGG - chr4_3:242930-242949 Msa0623080:CDS 30.0%
CTAATATATGATGTTGGTGT+AGG + chr4_3:242959-242978 None:intergenic 30.0%
AGGGAACTAATATATGATGT+TGG + chr4_3:242965-242984 None:intergenic 30.0%
! GAGATTTAAGGATAGCTTTT+AGG + chr4_3:242985-243004 None:intergenic 30.0%
ACAAAAGGAGTTCATTAGTT+TGG - chr4_3:243409-243428 Msa0623080:intron 30.0%
GTTTGGAGAATTGTATTCAA+TGG - chr4_3:243426-243445 Msa0623080:intron 30.0%
GAAAATGACGAAACAATTGA+CGG - chr4_3:243504-243523 Msa0623080:intron 30.0%
AAAATGACGAAACAATTGAC+GGG - chr4_3:243505-243524 Msa0623080:intron 30.0%
ATATTTGCAAAGCAGAATCA+AGG - chr4_3:243628-243647 Msa0623080:intron 30.0%
TGAGTATGGTTATGTTGATA+TGG - chr4_3:243678-243697 Msa0623080:intron 30.0%
ATGGTTATGTTGATATGGTT+AGG - chr4_3:243683-243702 Msa0623080:intron 30.0%
TGGTTATGTTGATATGGTTA+GGG - chr4_3:243684-243703 Msa0623080:intron 30.0%
TTCATATTGCTGCTAAACAA+GGG - chr4_3:243770-243789 Msa0623080:intron 30.0%
! GTTCTTTTGTGTTTTGTGAA+AGG - chr4_3:243838-243857 Msa0623080:intron 30.0%
AATCTCAGTTCTGTTGTTAT+TGG - chr4_3:244106-244125 Msa0623080:intron 30.0%
AACCTATTCACTTATCAGTA+AGG + chr4_3:244137-244156 None:intergenic 30.0%
! TTACTACGGTTTAGTTTACA+AGG + chr4_3:244167-244186 None:intergenic 30.0%
GTGAAATATCTATTAGAAGC+AGG - chr4_3:244462-244481 Msa0623080:CDS 30.0%
AGATCCATCAACAATAAACA+TGG - chr4_3:244686-244705 Msa0623080:intron 30.0%
TCAACCATGTTTATTGTTGA+TGG + chr4_3:244693-244712 None:intergenic 30.0%
AACTCAGGTAAATTTGACTA+TGG - chr4_3:244758-244777 Msa0623080:intron 30.0%
GACATGTTTATCAACAAAGA+TGG + chr4_3:244869-244888 None:intergenic 30.0%
CATGTCGTCACTTAAAATTT+CGG - chr4_3:244883-244902 Msa0623080:intron 30.0%
CTATGAAGTACAAACACTTA+CGG + chr4_3:245354-245373 None:intergenic 30.0%
!! CAGATTATCAAGTTGATACT+TGG - chr4_3:245551-245570 Msa0623080:five_prime_UTR 30.0%
!! AAAAGCATTCTTACAGAACA+CGG - chr4_3:245659-245678 Msa0623080:intron 30.0%
! TGTTTTCGGTTGAGATTTTA+TGG + chr4_3:245702-245721 None:intergenic 30.0%
AGTATTCATCGTGTTTGATT+CGG - chr4_3:246021-246040 Msa0623080:intron 30.0%
!!! GCTTATATCTGTGTCATTTT+TGG - chr4_3:246162-246181 Msa0623080:intron 30.0%
!!! GCTTTGTCTTTTTTAGTTGT+GGG - chr4_3:246184-246203 Msa0623080:intron 30.0%
!!! CTTTGTCTTTTTTAGTTGTG+GGG - chr4_3:246185-246204 Msa0623080:intron 30.0%
CAATTGGGGTAACTATTATT+GGG - chr4_3:246227-246246 Msa0623080:five_prime_UTR 30.0%
TATTATTGGGACTACCATAA+TGG - chr4_3:246240-246259 Msa0623080:five_prime_UTR 30.0%
ATTGGGTACTATGAGTTATT+GGG - chr4_3:246270-246289 Msa0623080:five_prime_UTR 30.0%
CTATGAGTTATTGGGTTATT+AGG - chr4_3:246278-246297 Msa0623080:five_prime_UTR 30.0%
TTAGGCATAGAATCAATTTG+AGG - chr4_3:246296-246315 Msa0623080:five_prime_UTR 30.0%
TTTAGTGGAACACACAAGTA+AGG - chr4_3:243013-243032 Msa0623080:CDS 35.0%
!! AGAGCAATGATTTTGGAGAA+AGG - chr4_3:243184-243203 Msa0623080:CDS 35.0%
AAATGACGAAACAATTGACG+GGG - chr4_3:243506-243525 Msa0623080:intron 35.0%
! TCTTTGAAGGATACAGTTGA+TGG - chr4_3:243580-243599 Msa0623080:intron 35.0%
TATTATGATCTTGCTGATGC+TGG - chr4_3:243718-243737 Msa0623080:intron 35.0%
GCTGGAATTAAAGCAAGAAA+TGG - chr4_3:243736-243755 Msa0623080:intron 35.0%
CTTCATATTGCTGCTAAACA+AGG - chr4_3:243769-243788 Msa0623080:intron 35.0%
TCATATTGCTGCTAAACAAG+GGG - chr4_3:243771-243790 Msa0623080:intron 35.0%
ACAAGGGGATTTAGGTATTA+TGG - chr4_3:243786-243805 Msa0623080:intron 35.0%
TTTGTGAAAGGAGTAATGAG+TGG - chr4_3:243850-243869 Msa0623080:intron 35.0%
TGTCTTCGCAATCATAATTG+AGG + chr4_3:243960-243979 None:intergenic 35.0%
GTGTCATAAAAATCCACTCA+AGG + chr4_3:244039-244058 None:intergenic 35.0%
AAAGCACAAAATGAGCAACT+TGG + chr4_3:244061-244080 None:intergenic 35.0%
TGCCTTACTGATAAGTGAAT+AGG - chr4_3:244132-244151 Msa0623080:intron 35.0%
AAACTGTTAGAACAAAACCC+TGG + chr4_3:244324-244343 None:intergenic 35.0%
TTCTGAATTGTCAATGACTG+TGG - chr4_3:244383-244402 Msa0623080:CDS 35.0%
GCAACAATTGCTAGAAGTAA+TGG - chr4_3:244495-244514 Msa0623080:CDS 35.0%
AAGATAGTGTTCACAAGAAG+TGG - chr4_3:244827-244846 Msa0623080:intron 35.0%
ATCTCACAAACTGTGTTTGT+AGG + chr4_3:244925-244944 None:intergenic 35.0%
TCTTTGTTCAAAGTGTGACT+TGG - chr4_3:244965-244984 Msa0623080:intron 35.0%
CTTTGTTCAAAGTGTGACTT+GGG - chr4_3:244966-244985 Msa0623080:intron 35.0%
! GTATGTGTAAGGGGTATATA+GGG + chr4_3:244993-245012 None:intergenic 35.0%
!! CCAATTTTGGTATGTGTAAG+GGG + chr4_3:245002-245021 None:intergenic 35.0%
!! CCCAATTTTGGTATGTGTAA+GGG + chr4_3:245003-245022 None:intergenic 35.0%
CCCTTACACATACCAAAATT+GGG - chr4_3:245000-245019 Msa0623080:intron 35.0%
TTGGTACTATCCTAGAACTT+GGG - chr4_3:245064-245083 Msa0623080:intron 35.0%
AGTGCTTTGTAGGATAATAG+CGG + chr4_3:245194-245213 None:intergenic 35.0%
TGTCAATGTCGTATATGTGT+CGG + chr4_3:245221-245240 None:intergenic 35.0%
ATGTAAAGAACATAGGACAC+CGG - chr4_3:245267-245286 Msa0623080:CDS 35.0%
TGAACTCGTATGATACCTAT+TGG + chr4_3:245295-245314 None:intergenic 35.0%
GTACAAACACTTACGGAATT+AGG + chr4_3:245347-245366 None:intergenic 35.0%
ACTTCATAGCTGCTAATTCT+TGG - chr4_3:245365-245384 Msa0623080:CDS 35.0%
CTTCATAGCTGCTAATTCTT+GGG - chr4_3:245366-245385 Msa0623080:CDS 35.0%
TTCATAGCTGCTAATTCTTG+GGG - chr4_3:245367-245386 Msa0623080:CDS 35.0%
TACCTATTTGGATTAAGTGC+TGG - chr4_3:245477-245496 Msa0623080:CDS 35.0%
ATCCAGCACTTAATCCAAAT+AGG + chr4_3:245482-245501 None:intergenic 35.0%
!! GATTAAGTGCTGGATTATTC+AGG - chr4_3:245487-245506 Msa0623080:CDS 35.0%
!! GCATTTCCAAAAGTTGATAG+AGG + chr4_3:245532-245551 None:intergenic 35.0%
CTTGGACAAAGTGAAACAAA+TGG - chr4_3:245569-245588 Msa0623080:five_prime_UTR 35.0%
ACAAAGTGAAACAAATGGCA+TGG - chr4_3:245574-245593 Msa0623080:five_prime_UTR 35.0%
CAAAGCAAAGAAGCAAATGA+TGG - chr4_3:246105-246124 Msa0623080:intron 35.0%
!!! GGCTTTGTCTTTTTTAGTTG+TGG - chr4_3:246183-246202 Msa0623080:intron 35.0%
TAGTTGTGGGGAAAAAACAA+AGG - chr4_3:246197-246216 Msa0623080:intron 35.0%
GCAATTGGGGTAACTATTAT+TGG - chr4_3:246226-246245 Msa0623080:five_prime_UTR 35.0%
TACCATAATGGCTACAACAT+TGG - chr4_3:246252-246271 Msa0623080:five_prime_UTR 35.0%
ACCATAATGGCTACAACATT+GGG - chr4_3:246253-246272 Msa0623080:five_prime_UTR 35.0%
CATTGGGTACTATGAGTTAT+TGG - chr4_3:246269-246288 Msa0623080:five_prime_UTR 35.0%
GAAAATCTTCAATGGGAAGT+AGG - chr4_3:246326-246345 Msa0623080:five_prime_UTR 35.0%
! AGCAAGTGTTGTGAAGTAGT+GGG + chr4_3:242542-242561 None:intergenic 40.0%
! AAGCAAGTGTTGTGAAGTAG+TGG + chr4_3:242543-242562 None:intergenic 40.0%
!! CACTACCTTTTGTTTCCTGT+TGG - chr4_3:242635-242654 Msa0623080:CDS 40.0%
ATGCACCAACAGGAAACAAA+AGG + chr4_3:242643-242662 None:intergenic 40.0%
TCAGATATACATGCACCAAC+AGG + chr4_3:242653-242672 None:intergenic 40.0%
TTTGCAAAGCAGAATCAAGG+TGG - chr4_3:243631-243650 Msa0623080:intron 40.0%
CTTTATGTTGCTGCTGAGTA+TGG - chr4_3:243664-243683 Msa0623080:intron 40.0%
ATTAGAAGCAGGTAGTAGCT+TGG - chr4_3:244473-244492 Msa0623080:CDS 40.0%
TTGCATTCTGCTGCAAGAAA+TGG - chr4_3:244528-244547 Msa0623080:CDS 40.0%
TGCTGCAAGAAATGGACATT+TGG - chr4_3:244536-244555 Msa0623080:CDS 40.0%
TGCAAGAAATGGACATTTGG+AGG - chr4_3:244539-244558 Msa0623080:CDS 40.0%
TGTGAAAGCGATTCTTGAGA+AGG - chr4_3:244563-244582 Msa0623080:CDS 40.0%
! TGTAACGCGGACTGATAAAA+AGG - chr4_3:244596-244615 Msa0623080:CDS 40.0%
! GTAACGCGGACTGATAAAAA+GGG - chr4_3:244597-244616 Msa0623080:CDS 40.0%
GACTGATAAAAAGGGACAGA+CGG - chr4_3:244605-244624 Msa0623080:CDS 40.0%
TTGCATATAGCAACCAGGAA+GGG - chr4_3:244732-244751 Msa0623080:intron 40.0%
! GGTATGTGTAAGGGGTATAT+AGG + chr4_3:244994-245013 None:intergenic 40.0%
CCCCTTACACATACCAAAAT+TGG - chr4_3:244999-245018 Msa0623080:intron 40.0%
!! GCCCAATTTTGGTATGTGTA+AGG + chr4_3:245004-245023 None:intergenic 40.0%
ACACATACCAAAATTGGGCT+TGG - chr4_3:245005-245024 Msa0623080:intron 40.0%
! TCACATACCAAGCCCAATTT+TGG + chr4_3:245015-245034 None:intergenic 40.0%
TGTTAGATGGCTTGATGAAC+AGG - chr4_3:245041-245060 Msa0623080:intron 40.0%
GTTGGTACTATCCTAGAACT+TGG - chr4_3:245063-245082 Msa0623080:intron 40.0%
TTAAGCACAACCCAAGTTCT+AGG + chr4_3:245077-245096 None:intergenic 40.0%
TGCTTTGTAGGATAATAGCG+GGG + chr4_3:245192-245211 None:intergenic 40.0%
GTGCTTTGTAGGATAATAGC+GGG + chr4_3:245193-245212 None:intergenic 40.0%
!! TCTCTTGTTGCAGCTGTTTT+CGG + chr4_3:245716-245735 None:intergenic 40.0%
TTTGACGAGTGTGTTCTAGT+TGG + chr4_3:245781-245800 None:intergenic 40.0%
CTCAACAAAATGCACACAGA+AGG - chr4_3:245833-245852 Msa0623080:intron 40.0%
! TTTCCTCGAGGTATGTGTTT+TGG + chr4_3:245959-245978 None:intergenic 40.0%
!!! AGCTATTTTTGCTTCGCCAA+GGG + chr4_3:245984-246003 None:intergenic 40.0%
!!! AAGCTATTTTTGCTTCGCCA+AGG + chr4_3:245985-246004 None:intergenic 40.0%
ACGATGAATACTAGAAACGC+GGG + chr4_3:246013-246032 None:intergenic 40.0%
CACGATGAATACTAGAAACG+CGG + chr4_3:246014-246033 None:intergenic 40.0%
TTGTGGGGAAAAAACAAAGG+TGG - chr4_3:246200-246219 Msa0623080:intron 40.0%
! AAACAAAGGTGGCTTGCAAT+TGG - chr4_3:246211-246230 Msa0623080:five_prime_UTR 40.0%
! AACAAAGGTGGCTTGCAATT+GGG - chr4_3:246212-246231 Msa0623080:five_prime_UTR 40.0%
ACCCAATGTTGTAGCCATTA+TGG + chr4_3:246257-246276 None:intergenic 40.0%
! CAAGTGTTGTGAAGTAGTGG+GGG + chr4_3:242540-242559 None:intergenic 45.0%
! GCAAGTGTTGTGAAGTAGTG+GGG + chr4_3:242541-242560 None:intergenic 45.0%
TTCTTCCTTTGAGCCCTTAC+TGG + chr4_3:243487-243506 None:intergenic 45.0%
CGATGATACGCCGTTACATT+CGG - chr4_3:243534-243553 Msa0623080:intron 45.0%
TGGGAACATGGCTTCTTTGA+AGG - chr4_3:243567-243586 Msa0623080:intron 45.0%
!! TACAGTTGATGGTGCTGAAG+AGG - chr4_3:243591-243610 Msa0623080:intron 45.0%
!! ACAGTTGATGGTGCTGAAGA+GGG - chr4_3:243592-243611 Msa0623080:intron 45.0%
GCTGCTAAACAAGGGGATTT+AGG - chr4_3:243778-243797 Msa0623080:intron 45.0%
TTGCGGTTGAAGTCATGTTG+CGG - chr4_3:243890-243909 Msa0623080:intron 45.0%
GTTGCATATAGCAACCAGGA+AGG - chr4_3:244731-244750 Msa0623080:intron 45.0%
AGATGGCTTGATGAACAGGT+TGG - chr4_3:245045-245064 Msa0623080:intron 45.0%
CGTATGATACCTATTGGTGC+CGG + chr4_3:245289-245308 None:intergenic 45.0%
GGCATGGCAGTAAACAAAAG+TGG - chr4_3:245590-245609 Msa0623080:intron 45.0%
CGAAACAGCATTAGACACAG+CGG - chr4_3:245613-245632 Msa0623080:intron 45.0%
TTAGACACAGCGGAGAAAAC+AGG - chr4_3:245623-245642 Msa0623080:intron 45.0%
!! GAGTGTGTTCTAGTTGGTGA+TGG + chr4_3:245775-245794 None:intergenic 45.0%
!! CAAACACGAAGAAGCGTTCA+AGG - chr4_3:245797-245816 Msa0623080:intron 45.0%
!! GTTTTGGATCGTCCACGAAT+TGG + chr4_3:245943-245962 None:intergenic 45.0%
GATCCAAAACACATACCTCG+AGG - chr4_3:245953-245972 Msa0623080:five_prime_UTR 45.0%
ATACCTCGAGGAAAATCCCT+TGG - chr4_3:245965-245984 Msa0623080:five_prime_UTR 45.0%
! ACAAAGGTGGCTTGCAATTG+GGG - chr4_3:246213-246232 Msa0623080:five_prime_UTR 45.0%
CTTCAATGGGAAGTAGGTCA+AGG - chr4_3:246332-246351 Msa0623080:five_prime_UTR 45.0%
!! TGTTAATATATTAATATTAA+CGG - chr4_3:242910-242929 Msa0623080:CDS 5.0%
! AGTAGTGGGGGCTAATGCAA+TGG + chr4_3:242528-242547 None:intergenic 50.0%
GCTCTTGCTGCCGAATGTAA+CGG + chr4_3:243547-243566 None:intergenic 50.0%
TTACATTCGGCAGCAAGAGC+TGG - chr4_3:243547-243566 Msa0623080:intron 50.0%
TACATTCGGCAGCAAGAGCT+GGG - chr4_3:243548-243567 Msa0623080:intron 50.0%
TGCAAGGCTGTTGTGTTGGA+CGG + chr4_3:244411-244430 None:intergenic 50.0%
AGTGTGCAAGGCTGTTGTGT+TGG + chr4_3:244415-244434 None:intergenic 50.0%
TTGTGTTGCAGCAGTGTGCA+AGG + chr4_3:244427-244446 None:intergenic 50.0%
TTGCACACTGCTGCAACACA+AGG - chr4_3:244426-244445 Msa0623080:CDS 50.0%
! GCAGAGTCTTGTGGTAGTAG+AGG - chr4_3:244650-244669 Msa0623080:intron 50.0%
CAGCGTTGCATATAGCAACC+AGG - chr4_3:244727-244746 Msa0623080:intron 50.0%
AACCAGGAAGGGAAGAACTC+AGG - chr4_3:244743-244762 Msa0623080:intron 50.0%
TACCTGAGTTCTTCCCTTCC+TGG + chr4_3:244748-244767 None:intergenic 50.0%
TGTCGGAGTCAGTGCTTTGT+AGG + chr4_3:245204-245223 None:intergenic 50.0%
AAACTCCACAACAGTCGTCG+CGG - chr4_3:245871-245890 Msa0623080:intron 50.0%
CGAAAGCAACCGTTGCTATG+AGG + chr4_3:245898-245917 None:intergenic 50.0%
AGCTGAAACTGTGCAGCCTC+AGG - chr4_3:243456-243475 Msa0623080:intron 55.0%
CTGCACCAGTAAGGGCTCAA+AGG - chr4_3:243479-243498 Msa0623080:intron 55.0%
GCGATTCTTGAGAAGGAGCC+AGG - chr4_3:244570-244589 Msa0623080:CDS 55.0%
! AGGAGCCAGGTGTTGTAACG+CGG - chr4_3:244583-244602 Msa0623080:CDS 55.0%
TCAGTCCGCGTTACAACACC+TGG + chr4_3:244591-244610 None:intergenic 55.0%
GCGCTTCACATGGCTGTGAA+GGG - chr4_3:244627-244646 Msa0623080:intron 55.0%
CGCTTCACATGGCTGTGAAG+GGG - chr4_3:244628-244647 Msa0623080:intron 55.0%
TGTGAAGGGGCAGAGTCTTG+TGG - chr4_3:244641-244660 Msa0623080:intron 55.0%
CATAGGACACCGGCACCAAT+AGG - chr4_3:245277-245296 Msa0623080:CDS 55.0%
! GATCGTCCACGAATTGGCCT+GGG + chr4_3:245937-245956 None:intergenic 55.0%
! TCGCCAAGGGATTTTCCTCG+AGG + chr4_3:245971-245990 None:intergenic 55.0%
TTTCATCTCCCTCGCGGTTG+TGG - chr4_3:246051-246070 Msa0623080:intron 55.0%
TTGAACCACCACAACCGCGA+GGG + chr4_3:246062-246081 None:intergenic 55.0%
TTTGAACCACCACAACCGCG+AGG + chr4_3:246063-246082 None:intergenic 55.0%
AGCCTCAGGCTGCACCAGTA+AGG - chr4_3:243470-243489 Msa0623080:intron 60.0%
GCCTCAGGCTGCACCAGTAA+GGG - chr4_3:243471-243490 Msa0623080:intron 60.0%
GGCAGCAAGAGCTGGGAACA+TGG - chr4_3:243555-243574 Msa0623080:intron 60.0%
GGCGCTTCACATGGCTGTGA+AGG - chr4_3:244626-244645 Msa0623080:intron 60.0%
CGTCGCGGTCCTCATAGCAA+CGG - chr4_3:245886-245905 Msa0623080:intron 60.0%
TTGGCCTGGGACGGTGAAGA+TGG + chr4_3:245924-245943 None:intergenic 60.0%
! GTCCACGAATTGGCCTGGGA+CGG + chr4_3:245933-245952 None:intergenic 60.0%
GGATCGTCCACGAATTGGCC+TGG + chr4_3:245938-245957 None:intergenic 60.0%
! CGCGCTTTTCATCTCCCTCG+CGG - chr4_3:246045-246064 Msa0623080:intron 60.0%
GCCCTTACTGGTGCAGCCTG+AGG + chr4_3:243475-243494 None:intergenic 65.0%
GGGACAGACGGCGCTTCACA+TGG - chr4_3:244617-244636 Msa0623080:CDS 65.0%
GAGGACCGCGACGACTGTTG+TGG + chr4_3:245879-245898 None:intergenic 65.0%
GCAGCCATCTTCACCGTCCC+AGG - chr4_3:245917-245936 Msa0623080:intron 65.0%
CACCGTCCCAGGCCAATTCG+TGG - chr4_3:245928-245947 Msa0623080:intron 65.0%
CATCTCCCTCGCGGTTGTGG+TGG - chr4_3:246054-246073 Msa0623080:intron 65.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 242486 246482 242486 ID=Msa0623080;Name=Msa0623080
chr4_3 mRNA 242486 246482 242486 ID=Msa0623080-mRNA-1;Parent=Msa0623080;Name=Msa0623080-mRNA-1;_AED=0.12;_eAED=0.12;_QI=491|0.8|0.83|1|0.4|0.33|6|53|556
chr4_3 exon 242486 243414 242486 ID=Msa0623080-mRNA-1:exon:48;Parent=Msa0623080-mRNA-1
chr4_3 exon 244204 244643 244204 ID=Msa0623080-mRNA-1:exon:47;Parent=Msa0623080-mRNA-1
chr4_3 exon 245169 245603 245169 ID=Msa0623080-mRNA-1:exon:46;Parent=Msa0623080-mRNA-1
chr4_3 exon 245735 245782 245735 ID=Msa0623080-mRNA-1:exon:45;Parent=Msa0623080-mRNA-1
chr4_3 exon 245934 246017 245934 ID=Msa0623080-mRNA-1:exon:44;Parent=Msa0623080-mRNA-1
chr4_3 exon 246204 246482 246204 ID=Msa0623080-mRNA-1:exon:43;Parent=Msa0623080-mRNA-1
chr4_3 five_prime_UTR 246204 246482 246204 ID=Msa0623080-mRNA-1:five_prime_utr;Parent=Msa0623080-mRNA-1
chr4_3 five_prime_UTR 245934 246017 245934 ID=Msa0623080-mRNA-1:five_prime_utr;Parent=Msa0623080-mRNA-1
chr4_3 five_prime_UTR 245735 245782 245735 ID=Msa0623080-mRNA-1:five_prime_utr;Parent=Msa0623080-mRNA-1
chr4_3 five_prime_UTR 245524 245603 245524 ID=Msa0623080-mRNA-1:five_prime_utr;Parent=Msa0623080-mRNA-1
chr4_3 CDS 245169 245523 245169 ID=Msa0623080-mRNA-1:cds;Parent=Msa0623080-mRNA-1
chr4_3 CDS 244204 244643 244204 ID=Msa0623080-mRNA-1:cds;Parent=Msa0623080-mRNA-1
chr4_3 CDS 242539 243414 242539 ID=Msa0623080-mRNA-1:cds;Parent=Msa0623080-mRNA-1
chr4_3 three_prime_UTR 242486 242538 242486 ID=Msa0623080-mRNA-1:three_prime_utr;Parent=Msa0623080-mRNA-1
Gene Sequence

>Msa0623080

ATGGACAAAGAAGCTGAAACTGTGCAGCCTCAGGCTGCACCAGTAAGGGCTCAAAGGAAGAAAATGACGAAACAATTGACGGGGAAGCGCGATGATACGCCGTTACATTCGGCAGCAAGAGCTGGGAACATGGCTTCTTTGAAGGATACAGTTGATGGTGCTGAAGAGGGTAAACTGCGCGAAATATTTGCAAAGCAGAATCAAGGTGGAGAAACAGCACTTTATGTTGCTGCTGAGTATGGTTATGTTGATATGGTTAGGGAGATGATTCAGTATTATGATCTTGCTGATGCTGGAATTAAAGCAAGAAATGGTTTTGATGCACTTCATATTGCTGCTAAACAAGGGGATTTAGGGTTTTGTTCTAACAGTTTCTTTGTTGTAGATATTGTAAAGATCTTAATGGAAGCTCATTCTGAATTGTCAATGACTGTGGATCCGTCCAACACAACAGCCTTGCACACTGCTGCAACACAAGGACATACTGAGATAGTGAAATATCTATTAGAAGCAGGTAGTAGCTTGGCAACAATTGCTAGAAGTAATGGTAAAACAGCTTTGCATTCTGCTGCAAGAAATGGACATTTGGAGGTTGTGAAAGCGATTCTTGAGAAGGAGCCAGGTGTTGTAACGCGGACTGATAAAAAGGGACAGACGGCGCTTCACATGGCTGTGAAGGGGCAGAGTCTTGTGGTAGTAGAGGAGTTGATAAAAGCAGATCCATCAACAATAAACATGGTTGATAATAAGGGTAATACAGCGTTGCATATAGCAACCAGGAAGGGAAGAACTCAGATTATCAAGTTGATACTTGGACAAAGTGAAACAAATGGCATGGCAGTAAACAAAAGTGGCGAAACAGCATTAGACACAGCGGAGAAAACAGGAAACTCTGAAGTAAAAAGCATTCTTACAGAACACGGTGTTCAAAGCAGCAAATCCATAAAATCTCAACCGAAAACAGCTGCAACAAGAGAATTAAAACAAACAGTCAGTGACATAAAGCATGAAGTCCATCACCAACTAGAACACACTCGTCAAACACGAAGAAGCGTTCAAGGAATAGCAAAACGTCTCAACAAAATGCACACAGAAGGACTCAACAATGCGATAAACTCCACAACAGTCGTCGCGGTCCTCATAGCAACGGTTGCTTTCGCAGCCATCTTCACCGTCCCAGGCCAATTCGTGGACGATCCAAAACACATACCTCGAGGAAAATCCCTTGGCGAAGCAAAAATAGCTTCAGAACCCGCGTTTCTAGTATTCATCGTGTTTGATTCGGTCGCGCTTTTCATCTCCCTCGCGGTTGTGGTGGTTCAAACATCAATAGTTGTTGTTGAAAGCAAAGCAAAGAAGCAAATGATGGCTATTATTAACAAACTAATGTGGTTAGCTTGTGTGCTTATATCTGTGTCATTTTTGGCTTTGTCTTTTTTAGTTGTGGGGAAAAAACAAAGGTGGCTTGCAATTGGGGTAACTATTATTGGGACTACCATAATGGCTACAACATTGGGTACTATGAGTTATTGGGTTATTAGGCATAGAATCAATTTGAGGAGTATAAGAAAATCTTCAATGGGAAGTAGGTCAAGGTCTTTTTCAGTTTCAGTTGTTATGTCTGATTCTGAGATATTAAACAATGAGCGTAATAAAATGTATGCTATTTAG
Protein sequence

>Msa0623080

MDKEAETVQPQAAPVRAQRKKMTKQLTGKRDDTPLHSAARAGNMASLKDTVDGAEEGKLREIFAKQNQGGETALYVAAEYGYVDMVREMIQYYDLADAGIKARNGFDALHIAAKQGDLGFCSNSFFVVDIVKILMEAHSELSMTVDPSNTTALHTAATQGHTEIVKYLLEAGSSLATIARSNGKTALHSAARNGHLEVVKAILEKEPGVVTRTDKKGQTALHMAVKGQSLVVVEELIKADPSTINMVDNKGNTALHIATRKGRTQIIKLILGQSETNGMAVNKSGETALDTAEKTGNSEVKSILTEHGVQSSKSIKSQPKTAATRELKQTVSDIKHEVHHQLEHTRQTRRSVQGIAKRLNKMHTEGLNNAINSTTVVAVLIATVAFAAIFTVPGQFVDDPKHIPRGKSLGEAKIASEPAFLVFIVFDSVALFISLAVVVVQTSIVVVESKAKKQMMAIINKLMWLACVLISVSFLALSFLVVGKKQRWLAIGVTIIGTTIMATTLGTMSYWVIRHRINLRSIRKSSMGSRSRSFSVSVVMSDSEILNNERNKMYAI*