Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0312160 | ABN08111.1 | 83.796 | 216 | 7 | 2 | 1 | 188 | 345 | 560 | 2.51e-122 | 367 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0312160 | sp|Q501D1|Y1490_ARATH | 39.908 | 218 | 91 | 3 | 3 | 187 | 360 | 570 | 6.78e-48 | 167 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0017510 | Msa0312160 | 0.810162 | 1.322267e-50 | -8.615850e-47 |
| Msa0079080 | Msa0312160 | 0.801949 | 7.072946e-49 | -8.615850e-47 |
| Msa0110970 | Msa0312160 | 0.803553 | 3.299497e-49 | -8.615850e-47 |
| Msa0157290 | Msa0312160 | 0.810776 | 9.743228e-51 | -8.615850e-47 |
| Msa0243100 | Msa0312160 | 0.803991 | 2.676963e-49 | -8.615850e-47 |
| Msa0312160 | Msa0376350 | 0.800365 | 1.491202e-48 | -8.615850e-47 |
| Msa0312160 | Msa0424790 | 0.803170 | 3.961365e-49 | -8.615850e-47 |
| Msa0312160 | Msa0565730 | 0.805713 | 1.168781e-49 | -8.615850e-47 |
| Msa0312160 | Msa0662500 | 0.802813 | 4.695436e-49 | -8.615850e-47 |
| Msa0312160 | Msa0861870 | 0.801759 | 7.738840e-49 | -8.615850e-47 |
| Msa0312160 | Msa0880140 | 0.806650 | 7.422112e-50 | -8.615850e-47 |
| Msa0312160 | Msa0935670 | 0.806526 | 7.880507e-50 | -8.615850e-47 |
| Msa0312160 | Msa0947660 | 0.800468 | 1.421176e-48 | -8.615850e-47 |
| Msa0312160 | Msa1154710 | 0.808103 | 3.650569e-50 | -8.615850e-47 |
| Msa0312160 | Msa1209460 | 0.819105 | 1.383381e-52 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0312160 | MtrunA17_Chr2g0309171 | 83.962 | 212 | 6 | 2 | 1 | 184 | 319 | 530 | 9.78e-118 | 359 |
| Msa0312160 | MtrunA17_Chr2g0309171 | 69.907 | 216 | 37 | 2 | 1 | 188 | 937 | 1152 | 8.14e-101 | 314 |
| Msa0312160 | MtrunA17_Chr2g0309181 | 71.296 | 216 | 30 | 3 | 1 | 188 | 375 | 586 | 1.01e-103 | 310 |
| Msa0312160 | MtrunA17_Chr2g0309191 | 62.500 | 216 | 44 | 3 | 1 | 188 | 347 | 553 | 6.93e-88 | 268 |
| Msa0312160 | MtrunA17_Chr4g0074491 | 60.648 | 216 | 57 | 2 | 1 | 188 | 332 | 547 | 9.99e-85 | 259 |
| Msa0312160 | MtrunA17_Chr2g0310081 | 47.706 | 218 | 66 | 5 | 1 | 188 | 380 | 579 | 1.65e-60 | 197 |
| Msa0312160 | MtrunA17_Chr5g0395701 | 45.161 | 217 | 80 | 3 | 2 | 187 | 397 | 605 | 5.91e-57 | 188 |
| Msa0312160 | MtrunA17_Chr1g0190341 | 46.083 | 217 | 77 | 4 | 3 | 187 | 350 | 558 | 1.59e-56 | 186 |
| Msa0312160 | MtrunA17_Chr1g0190371 | 46.083 | 217 | 77 | 4 | 3 | 187 | 350 | 558 | 1.59e-56 | 186 |
| Msa0312160 | MtrunA17_Chr4g0039581 | 43.519 | 216 | 83 | 3 | 3 | 187 | 360 | 567 | 5.66e-51 | 172 |
| Msa0312160 | MtrunA17_Chr2g0309131 | 42.925 | 212 | 54 | 5 | 1 | 187 | 316 | 485 | 5.15e-48 | 162 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0312160 | AT2G44260.1 | 46.296 | 216 | 81 | 4 | 1 | 187 | 343 | 552 | 2.22e-59 | 194 |
| Msa0312160 | AT2G44260.2 | 46.296 | 216 | 81 | 4 | 1 | 187 | 373 | 582 | 4.28e-59 | 194 |
| Msa0312160 | AT2G44230.1 | 46.330 | 218 | 80 | 4 | 1 | 188 | 332 | 542 | 1.81e-58 | 192 |
| Msa0312160 | AT3G04350.2 | 42.202 | 218 | 86 | 4 | 3 | 188 | 335 | 544 | 1.08e-53 | 179 |
| Msa0312160 | AT3G01870.1 | 44.131 | 213 | 85 | 5 | 1 | 187 | 379 | 583 | 1.27e-53 | 179 |
| Msa0312160 | AT3G04350.1 | 42.202 | 218 | 86 | 4 | 3 | 188 | 357 | 566 | 2.15e-53 | 179 |
| Msa0312160 | AT3G01880.1 | 44.131 | 213 | 86 | 5 | 1 | 187 | 384 | 589 | 1.19e-52 | 177 |
| Msa0312160 | AT5G18490.1 | 42.202 | 218 | 84 | 5 | 3 | 188 | 345 | 552 | 2.23e-51 | 173 |
| Msa0312160 | AT5G43950.1 | 40.553 | 217 | 91 | 3 | 2 | 187 | 355 | 564 | 2.37e-50 | 171 |
| Msa0312160 | AT1G04090.1 | 39.908 | 218 | 91 | 3 | 3 | 187 | 360 | 570 | 6.90e-49 | 167 |
Find 48 sgRNAs with CRISPR-Local
Find 62 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCCTCTCCCAAGGCTTCTTT+TGG | 0.234375 | 2_4:+7204177 | None:intergenic |
| TTGGTTTCTGCAGAATATTT+AGG | 0.246737 | 2_4:-7204348 | Msa0312160:intron |
| GAGTTTATAGATATTAAATT+AGG | 0.262170 | 2_4:-7204679 | Msa0312160:CDS |
| CTCAACACAATGCTGGTAAA+TGG | 0.273775 | 2_4:-7204570 | Msa0312160:CDS |
| ATTGGGAGCATGTGACATTA+AGG | 0.285039 | 2_4:-7204633 | Msa0312160:CDS |
| TGGTTTCTGCAGAATATTTA+GGG | 0.297210 | 2_4:-7204347 | Msa0312160:intron |
| AACTCTACTTTAGCTCTTCC+AGG | 0.339774 | 2_4:+7204697 | None:intergenic |
| GTAACTTCGATGGTCAATTA+TGG | 0.344009 | 2_4:-7204606 | Msa0312160:CDS |
| GTCCAAAGGAAAAGGGTAAT+TGG | 0.344024 | 2_4:-7204155 | Msa0312160:CDS |
| CATGCTGGCCTTAATCTACT+AGG | 0.360540 | 2_4:-7204442 | Msa0312160:CDS |
| AATGTGGCTAAGGCGTTGCC+TGG | 0.415179 | 2_4:-7204240 | Msa0312160:CDS |
| ATTGTTTATGCTTCTTTGCA+TGG | 0.417458 | 2_4:-7204481 | Msa0312160:CDS |
| TTCCTTTGGACCCTCTCCCA+AGG | 0.455897 | 2_4:+7204167 | None:intergenic |
| GGGAGAGGGTCCAAAGGAAA+AGG | 0.460332 | 2_4:-7204163 | Msa0312160:CDS |
| ACCAAAAGAAGCCTTGGGAG+AGG | 0.462759 | 2_4:-7204178 | Msa0312160:CDS |
| GATATTAAATTAGGAAAAGC+AGG | 0.463543 | 2_4:-7204670 | Msa0312160:CDS |
| TGCAAAGAAGCATAAACAAT+AGG | 0.464083 | 2_4:+7204484 | None:intergenic |
| CAGGTGAACATGTTGGTGAT+TGG | 0.472172 | 2_4:-7204651 | Msa0312160:CDS |
| CAAAACTACTAGCATCACTT+TGG | 0.490875 | 2_4:+7204524 | None:intergenic |
| GAGTTTACCAAAAGAAGCCT+TGG | 0.494113 | 2_4:-7204184 | Msa0312160:CDS |
| ATTCACTGATATTGCTATGT+GGG | 0.497104 | 2_4:-7204740 | Msa0312160:CDS |
| CTAAAATAATTCAACCATGC+TGG | 0.503659 | 2_4:+7204309 | None:intergenic |
| AGGTGAACATGTTGGTGATT+GGG | 0.508342 | 2_4:-7204650 | Msa0312160:CDS |
| GCTCTTCCAGGTCCATTGAA+AGG | 0.515609 | 2_4:+7204709 | None:intergenic |
| AGTTTACCAAAAGAAGCCTT+GGG | 0.519013 | 2_4:-7204183 | Msa0312160:CDS |
| TTGGGAGCATGTGACATTAA+GGG | 0.526297 | 2_4:-7204632 | Msa0312160:CDS |
| TCTAGATCAAAGTCAATATG+TGG | 0.527060 | 2_4:+7204276 | None:intergenic |
| AAACCATCTCTTAACTTCCC+AGG | 0.534968 | 2_4:+7204222 | None:intergenic |
| GTGAATGTTCCTCCATACAT+TGG | 0.538131 | 2_4:+7204757 | None:intergenic |
| TACTTCTCTCAACACAATGC+TGG | 0.544284 | 2_4:-7204577 | Msa0312160:CDS |
| GTACATGTGAAACCAATGTA+TGG | 0.546199 | 2_4:-7204769 | None:intergenic |
| CATTCACTGATATTGCTATG+TGG | 0.559656 | 2_4:-7204741 | Msa0312160:CDS |
| CCAAAAGAAGCCTTGGGAGA+GGG | 0.560515 | 2_4:-7204177 | Msa0312160:CDS |
| CATGCATCATATCCACATGC+TGG | 0.566284 | 2_4:-7204457 | Msa0312160:CDS |
| AAGGAAAAGGGTAATTGGAA+TGG | 0.567477 | 2_4:-7204150 | Msa0312160:CDS |
| ATGCTGGCCTTAATCTACTA+GGG | 0.568045 | 2_4:-7204441 | Msa0312160:CDS |
| GGAGAGGGTCCAAAGGAAAA+GGG | 0.568766 | 2_4:-7204162 | Msa0312160:CDS |
| TGAATTGCAGAATGTGGCTA+AGG | 0.571192 | 2_4:-7204250 | Msa0312160:CDS |
| ATGTGGCTAAGGCGTTGCCT+GGG | 0.589419 | 2_4:-7204239 | Msa0312160:CDS |
| TTGCCTGGGAAGTTAAGAGA+TGG | 0.591065 | 2_4:-7204225 | Msa0312160:CDS |
| CTGAAGTTAAAGAGCCAGCA+TGG | 0.591737 | 2_4:-7204323 | Msa0312160:CDS |
| GGAAAAGCAGGTGAACATGT+TGG | 0.601860 | 2_4:-7204658 | Msa0312160:CDS |
| AGATATTGAATTGCAGAATG+TGG | 0.619484 | 2_4:-7204256 | Msa0312160:CDS |
| AGCCTTGGGAGAGGGTCCAA+AGG | 0.628021 | 2_4:-7204169 | Msa0312160:CDS |
| CAGCATGTGGATATGATGCA+TGG | 0.658754 | 2_4:+7204458 | None:intergenic |
| AGTAGATTAAGGCCAGCATG+TGG | 0.670839 | 2_4:+7204445 | None:intergenic |
| TTAAGGGTAAGTAACTTCGA+TGG | 0.678056 | 2_4:-7204616 | Msa0312160:CDS |
| CATGTGAAACCAATGTATGG+AGG | 0.726903 | 2_4:-7204766 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GAGTTTATAGATATTAAATT+AGG | - | chr2_4:7204212-7204231 | Msa0312160:CDS | 15.0% |
| !! | TAATAAAGGTAGAAATGTTA+TGG | - | chr2_4:7204503-7204522 | Msa0312160:CDS | 20.0% |
| !!! | ATGGTTTTGAAAGTATTATT+AGG | - | chr2_4:7204685-7204704 | Msa0312160:CDS | 20.0% |
| ! | GATATTAAATTAGGAAAAGC+AGG | - | chr2_4:7204221-7204240 | Msa0312160:CDS | 25.0% |
| !! | TAAACAATAGGTCTTTTTGT+TGG | + | chr2_4:7204398-7204417 | None:intergenic | 25.0% |
| !!! | AGCATGGTTGAATTATTTTA+GGG | - | chr2_4:7204584-7204603 | Msa0312160:CDS | 25.0% |
| ATTCACTGATATTGCTATGT+GGG | - | chr2_4:7204151-7204170 | Msa0312160:CDS | 30.0% | |
| !!! | TGGGTTTTTTATCCTTTCAA+TGG | - | chr2_4:7204170-7204189 | Msa0312160:CDS | 30.0% |
| ! | TGCAAAGAAGCATAAACAAT+AGG | + | chr2_4:7204410-7204429 | None:intergenic | 30.0% |
| ATTGTTTATGCTTCTTTGCA+TGG | - | chr2_4:7204410-7204429 | Msa0312160:intron | 30.0% | |
| ! | CATTTTTCCCTAGTAGATTA+AGG | + | chr2_4:7204460-7204479 | None:intergenic | 30.0% |
| AGGTAGAAATGTTATGGATA+TGG | - | chr2_4:7204509-7204528 | Msa0312160:CDS | 30.0% | |
| GGTAGAAATGTTATGGATAT+GGG | - | chr2_4:7204510-7204529 | Msa0312160:CDS | 30.0% | |
| GGATATGGGAAAATATGTAT+TGG | - | chr2_4:7204524-7204543 | Msa0312160:CDS | 30.0% | |
| TTGGTTTCTGCAGAATATTT+AGG | - | chr2_4:7204543-7204562 | Msa0312160:CDS | 30.0% | |
| TGGTTTCTGCAGAATATTTA+GGG | - | chr2_4:7204544-7204563 | Msa0312160:CDS | 30.0% | |
| CTAAAATAATTCAACCATGC+TGG | + | chr2_4:7204585-7204604 | None:intergenic | 30.0% | |
| ! | CAGCATGGTTGAATTATTTT+AGG | - | chr2_4:7204583-7204602 | Msa0312160:CDS | 30.0% |
| !!! | GTTGAATTATTTTAGGGAGT+GGG | - | chr2_4:7204590-7204609 | Msa0312160:CDS | 30.0% |
| !!! | TTGAATTATTTTAGGGAGTG+GGG | - | chr2_4:7204591-7204610 | Msa0312160:CDS | 30.0% |
| TCTAGATCAAAGTCAATATG+TGG | + | chr2_4:7204618-7204637 | None:intergenic | 30.0% | |
| AGATATTGAATTGCAGAATG+TGG | - | chr2_4:7204635-7204654 | Msa0312160:CDS | 30.0% | |
| CATTCACTGATATTGCTATG+TGG | - | chr2_4:7204150-7204169 | Msa0312160:CDS | 35.0% | |
| ! | TTTTATCCTTTCAATGGACC+TGG | - | chr2_4:7204176-7204195 | Msa0312160:CDS | 35.0% |
| TTAAGGGTAAGTAACTTCGA+TGG | - | chr2_4:7204275-7204294 | Msa0312160:CDS | 35.0% | |
| GTAACTTCGATGGTCAATTA+TGG | - | chr2_4:7204285-7204304 | Msa0312160:CDS | 35.0% | |
| CAAAACTACTAGCATCACTT+TGG | + | chr2_4:7204370-7204389 | None:intergenic | 35.0% | |
| TACTAGGGAAAAATGTGAGT+AGG | - | chr2_4:7204465-7204484 | Msa0312160:CDS | 35.0% | |
| !!! | GGTTGAATTATTTTAGGGAG+TGG | - | chr2_4:7204589-7204608 | Msa0312160:CDS | 35.0% |
| AGTTTACCAAAAGAAGCCTT+GGG | - | chr2_4:7204708-7204727 | Msa0312160:CDS | 35.0% | |
| ! | TTCCAATTACCCTTTTCCTT+TGG | + | chr2_4:7204741-7204760 | None:intergenic | 35.0% |
| AAGGAAAAGGGTAATTGGAA+TGG | - | chr2_4:7204741-7204760 | Msa0312160:CDS | 35.0% | |
| !! | AACTCTACTTTAGCTCTTCC+AGG | + | chr2_4:7204197-7204216 | None:intergenic | 40.0% |
| !! | AGGTGAACATGTTGGTGATT+GGG | - | chr2_4:7204241-7204260 | Msa0312160:CDS | 40.0% |
| ! | ATTGGGAGCATGTGACATTA+AGG | - | chr2_4:7204258-7204277 | Msa0312160:CDS | 40.0% |
| ! | TTGGGAGCATGTGACATTAA+GGG | - | chr2_4:7204259-7204278 | Msa0312160:CDS | 40.0% |
| TACTTCTCTCAACACAATGC+TGG | - | chr2_4:7204314-7204333 | Msa0312160:CDS | 40.0% | |
| CTCAACACAATGCTGGTAAA+TGG | - | chr2_4:7204321-7204340 | Msa0312160:CDS | 40.0% | |
| !! | ATGCTGGCCTTAATCTACTA+GGG | - | chr2_4:7204450-7204469 | Msa0312160:CDS | 40.0% |
| GCACGAGATGACACTAATAA+AGG | - | chr2_4:7204489-7204508 | Msa0312160:CDS | 40.0% | |
| TGAATTGCAGAATGTGGCTA+AGG | - | chr2_4:7204641-7204660 | Msa0312160:CDS | 40.0% | |
| AAACCATCTCTTAACTTCCC+AGG | + | chr2_4:7204672-7204691 | None:intergenic | 40.0% | |
| GAGTTTACCAAAAGAAGCCT+TGG | - | chr2_4:7204707-7204726 | Msa0312160:CDS | 40.0% | |
| GTCCAAAGGAAAAGGGTAAT+TGG | - | chr2_4:7204736-7204755 | Msa0312160:CDS | 40.0% | |
| GGAAAAGCAGGTGAACATGT+TGG | - | chr2_4:7204233-7204252 | Msa0312160:CDS | 45.0% | |
| !! | CAGGTGAACATGTTGGTGAT+TGG | - | chr2_4:7204240-7204259 | Msa0312160:CDS | 45.0% |
| CAGCATGTGGATATGATGCA+TGG | + | chr2_4:7204436-7204455 | None:intergenic | 45.0% | |
| CATGCATCATATCCACATGC+TGG | - | chr2_4:7204434-7204453 | Msa0312160:CDS | 45.0% | |
| AGTAGATTAAGGCCAGCATG+TGG | + | chr2_4:7204449-7204468 | None:intergenic | 45.0% | |
| !! | CATGCTGGCCTTAATCTACT+AGG | - | chr2_4:7204449-7204468 | Msa0312160:CDS | 45.0% |
| CTGAAGTTAAAGAGCCAGCA+TGG | - | chr2_4:7204568-7204587 | Msa0312160:CDS | 45.0% | |
| TTGCCTGGGAAGTTAAGAGA+TGG | - | chr2_4:7204666-7204685 | Msa0312160:CDS | 45.0% | |
| GCTCTTCCAGGTCCATTGAA+AGG | + | chr2_4:7204185-7204204 | None:intergenic | 50.0% | |
| ACCAAAAGAAGCCTTGGGAG+AGG | - | chr2_4:7204713-7204732 | Msa0312160:CDS | 50.0% | |
| CCAAAAGAAGCCTTGGGAGA+GGG | - | chr2_4:7204714-7204733 | Msa0312160:CDS | 50.0% | |
| GGAGAGGGTCCAAAGGAAAA+GGG | - | chr2_4:7204729-7204748 | Msa0312160:CDS | 50.0% | |
| !! | AATGTGGCTAAGGCGTTGCC+TGG | - | chr2_4:7204651-7204670 | Msa0312160:CDS | 55.0% |
| !! | ATGTGGCTAAGGCGTTGCCT+GGG | - | chr2_4:7204652-7204671 | Msa0312160:CDS | 55.0% |
| ! | CCCTCTCCCAAGGCTTCTTT+TGG | + | chr2_4:7204717-7204736 | None:intergenic | 55.0% |
| TTCCTTTGGACCCTCTCCCA+AGG | + | chr2_4:7204727-7204746 | None:intergenic | 55.0% | |
| GGGAGAGGGTCCAAAGGAAA+AGG | - | chr2_4:7204728-7204747 | Msa0312160:CDS | 55.0% | |
| AGCCTTGGGAGAGGGTCCAA+AGG | - | chr2_4:7204722-7204741 | Msa0312160:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_4 | gene | 7204137 | 7204776 | 7204137 | ID=Msa0312160;Name=Msa0312160 |
| chr2_4 | mRNA | 7204137 | 7204776 | 7204137 | ID=Msa0312160-mRNA-1;Parent=Msa0312160;Name=Msa0312160-mRNA-1;_AED=0.44;_eAED=1.00;_QI=0|0|0|1|1|1|2|0|188 |
| chr2_4 | exon | 7204431 | 7204776 | 7204431 | ID=Msa0312160-mRNA-1:exon:2801;Parent=Msa0312160-mRNA-1 |
| chr2_4 | exon | 7204137 | 7204357 | 7204137 | ID=Msa0312160-mRNA-1:exon:2800;Parent=Msa0312160-mRNA-1 |
| chr2_4 | CDS | 7204431 | 7204776 | 7204431 | ID=Msa0312160-mRNA-1:cds;Parent=Msa0312160-mRNA-1 |
| chr2_4 | CDS | 7204137 | 7204357 | 7204137 | ID=Msa0312160-mRNA-1:cds;Parent=Msa0312160-mRNA-1 |
| Gene Sequence |
| Protein sequence |
