AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0320180

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0320180 MtrunA17_Chr2g0295091 96.815 157 5 0 1 157 1 157 2.52e-107 302
Msa0320180 MtrunA17_Chr2g0295141 95.541 157 7 0 1 157 1 157 6.33e-107 301
Msa0320180 MtrunA17_Chr2g0295104 91.720 157 13 0 1 157 1 157 9.48e-102 288
Msa0320180 MtrunA17_Chr2g0295124 89.809 157 16 0 1 157 1 157 1.07e-98 281
Msa0320180 MtrunA17_Chr6g0462311 66.242 157 53 0 1 157 1 157 2.21e-70 209
Msa0320180 MtrunA17_Chr4g0067951 66.242 157 53 0 1 157 1 157 7.13e-70 208
Msa0320180 MtrunA17_Chr2g0295021 63.694 157 57 0 1 157 1 157 7.49e-68 202
Msa0320180 MtrunA17_Chr2g0295001 63.694 157 57 0 1 157 1 157 7.49e-68 202
Msa0320180 MtrunA17_Chr2g0295064 63.694 157 57 0 1 157 68 224 3.36e-66 201
Msa0320180 MtrunA17_Chr2g0295051 61.783 157 60 0 1 157 1 157 1.29e-65 197
Msa0320180 MtrunA17_Chr2g0295041 59.236 157 64 0 1 157 1 157 2.48e-62 189
Msa0320180 MtrunA17_Chr6g0462291 64.286 140 50 0 1 140 1 140 2.19e-59 181
Msa0320180 MtrunA17_Chr2g0295081 49.045 157 80 0 1 157 1 157 7.52e-50 157
Msa0320180 MtrunA17_Chr4g0067921 42.857 161 85 5 1 157 1 158 2.84e-32 112
Msa0320180 MtrunA17_Chr2g0295211 32.051 156 105 1 1 155 1 156 6.49e-30 106
Msa0320180 MtrunA17_Chr4g0067841 33.758 157 103 1 1 156 1 157 1.08e-29 106
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 63 sgRNAs with CRISPR-Local

Find 180 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCTATTGAGGACTTTAATT+TGG 0.098681 2_4:+20471834 Msa0320180:three_prime_UTR
GGTTTAATTGGCTAGTAATT+AGG 0.164037 2_4:-20471813 None:intergenic
GTGAGGTTTGAGTGCTATTT+TGG 0.186747 2_4:+20471967 Msa0320180:three_prime_UTR
AGTCCTCAATAGGGTTTAAT+TGG 0.205101 2_4:-20471825 None:intergenic
GTTTAATTGGCTAGTAATTA+GGG 0.278519 2_4:-20471812 None:intergenic
GGATAGCTTCTTAATAGTTC+CGG 0.311830 2_4:-20470926 None:intergenic
TACAGCTTAGTGGGAGGAAC+AGG 0.324066 2_4:+20471597 Msa0320180:CDS
CCTATTGAGGACTTTAATTT+GGG 0.325571 2_4:+20471835 Msa0320180:three_prime_UTR
CTTGATAAGTCCAGTTCCTT+TGG 0.329858 2_4:-20471763 None:intergenic
TACATGTGTGTTGGCTTTGT+TGG 0.344945 2_4:+20471939 Msa0320180:three_prime_UTR
TTCAACAATTTCAACACTTT+GGG 0.350751 2_4:-20470890 None:intergenic
CCCAAATTAAAGTCCTCAAT+AGG 0.362125 2_4:-20471835 None:intergenic
CTTCAACAATTTCAACACTT+TGG 0.364915 2_4:-20470891 None:intergenic
TGAGTATACATCAAGGTCTT+TGG 0.372404 2_4:+20471912 Msa0320180:three_prime_UTR
TGGGCAGCAGAAATCACCTT+TGG 0.387864 2_4:-20470871 None:intergenic
AGATAGAGTTGCATCACCTT+TGG 0.394288 2_4:-20471715 None:intergenic
GATAGCTTCTTAATAGTTCC+GGG 0.397748 2_4:-20470925 None:intergenic
GTTGAAATTGTTGAAGGAAA+TGG 0.402462 2_4:+20470898 Msa0320180:CDS
CCTATGTTGTGTTGGTGATA+AGG 0.406752 2_4:+20472007 Msa0320180:three_prime_UTR
CCAAATTAAAGTCCTCAATA+GGG 0.411845 2_4:-20471834 None:intergenic
GAGACTAAGGCCAAAGGAAC+TGG 0.415366 2_4:+20471753 Msa0320180:CDS
AGAGCCTTGTAGAGTTTAGC+TGG 0.426244 2_4:-20470823 None:intergenic
GGGCAGCAGAAATCACCTTT+GGG 0.449440 2_4:-20470870 None:intergenic
TCAGTGGATGAGGCAAACTT+TGG 0.464386 2_4:+20471567 Msa0320180:CDS
TGCTAAAACGTAGCCCTCAA+TGG 0.474394 2_4:-20471787 None:intergenic
TGGATATAACTACAGCTTAG+TGG 0.476164 2_4:+20471587 Msa0320180:CDS
AAAATTGTTGCTGGCTCTGA+TGG 0.476544 2_4:+20471657 Msa0320180:CDS
TTTGGCTCGTACATGTGTGT+TGG 0.491488 2_4:+20471930 Msa0320180:three_prime_UTR
ATTAAATTAAATTAAAATGA+CGG 0.493877 2_4:-20472085 None:intergenic
CAAAGTGTTGAAATTGTTGA+AGG 0.502542 2_4:+20470892 Msa0320180:CDS
CAAATTAGATTCAGTGGATG+AGG 0.505903 2_4:+20471557 Msa0320180:CDS
GGATATAACTACAGCTTAGT+GGG 0.506709 2_4:+20471588 Msa0320180:CDS
ATCTGAATTACCTTCAACAA+TGG 0.509250 2_4:-20470947 None:intergenic
TAATTTGTGTAGCACAAAGT+TGG 0.512097 2_4:-20471541 None:intergenic
CCTTATCACCAACACAACAT+AGG 0.514089 2_4:-20472007 None:intergenic
GGACTTATCAAGGCCATTGA+GGG 0.514208 2_4:+20471774 Msa0320180:CDS
TATTTATTGCATAATACAGA+TGG 0.515188 2_4:-20472037 None:intergenic
CAAAGGAACTGGACTTATCA+AGG 0.516806 2_4:+20471764 Msa0320180:CDS
AAGAAGCTATCCATTGTTGA+AGG 0.523757 2_4:+20470937 Msa0320180:CDS
AGCAGTACGTGAGGAGACTA+AGG 0.536209 2_4:+20471740 Msa0320180:CDS
GAGTTGCATCACCTTTGGTA+TGG 0.537356 2_4:-20471710 None:intergenic
TAGCCAATTAAACCCTATTG+AGG 0.542857 2_4:+20471822 Msa0320180:exon
AATAATTAAAGTTTATGATG+CGG 0.546841 2_4:+20471878 Msa0320180:three_prime_UTR
GGCTCCAGCTAAACTCTACA+AGG 0.556705 2_4:+20470819 Msa0320180:CDS
GCTACACAAATTAGATTCAG+TGG 0.559555 2_4:+20471551 Msa0320180:CDS
TTTCACTGAAATCTTAACAA+TGG 0.567421 2_4:-20471685 None:intergenic
ATTGTTGCTGGCTCTGATGG+TGG 0.570543 2_4:+20471660 Msa0320180:CDS
TGGCTTTGTTGGATGTTGTG+AGG 0.571354 2_4:+20471950 Msa0320180:three_prime_UTR
TGGACTTATCAAGGCCATTG+AGG 0.572485 2_4:+20471773 Msa0320180:CDS
GTTGAAGGAAATGGAGGACC+CGG 0.599694 2_4:+20470907 Msa0320180:CDS
GTAGAGTTTAGCTGGAGCCA+CGG 0.602676 2_4:-20470815 None:intergenic
TGCTGATGAAATCGTCCCAA+AGG 0.606260 2_4:+20470855 Msa0320180:CDS
CTTAGTGGGAGGAACAGGGT+TGG 0.609437 2_4:+20471602 Msa0320180:CDS
ATATGCAAGAAGTAAATGAA+AGG 0.619747 2_4:-20470725 None:intergenic
GAAATTGTTGAAGGAAATGG+AGG 0.626795 2_4:+20470901 Msa0320180:CDS
TCAGTGAAATACCATACCAA+AGG 0.652605 2_4:+20471699 Msa0320180:CDS
ATATATATATCAAGATAACA+AGG 0.670405 2_4:-20470752 None:intergenic
CGTGAGGAGACTAAGGCCAA+AGG 0.670663 2_4:+20471747 Msa0320180:CDS
TCTATCTGAAGCAGTACGTG+AGG 0.676642 2_4:+20471731 Msa0320180:CDS
AGTGTGTTGAGTATACATCA+AGG 0.679518 2_4:+20471905 Msa0320180:three_prime_UTR
ACAGCTTAGTGGGAGGAACA+GGG 0.680453 2_4:+20471598 Msa0320180:CDS
TATAACTACAGCTTAGTGGG+AGG 0.697586 2_4:+20471591 Msa0320180:CDS
TGATGAACATGTCTCAACCG+TGG 0.717160 2_4:+20470798 Msa0320180:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTATATAAATAATTATAA+TGG + chr2_4:20471498-20471517 Msa0320180:intron 0.0%
!! AATTATATAAATAATTATAA+TGG + chr2_4:20471498-20471517 Msa0320180:intron 0.0%
!!! TTTGTCAATTTTTAAATAAA+AGG - chr2_4:20471092-20471111 None:intergenic 10.0%
!!! AAATAAAAGTATAACATAAA+TGG - chr2_4:20471303-20471322 None:intergenic 10.0%
!!! TTTGTCAATTTTTAAATAAA+AGG - chr2_4:20471092-20471111 None:intergenic 10.0%
!!! AAATAAAAGTATAACATAAA+TGG - chr2_4:20471303-20471322 None:intergenic 10.0%
!! ATATATATATCAAGATAACA+AGG - chr2_4:20470755-20470774 None:intergenic 15.0%
!! ATCTTGATATATATATATCA+TGG + chr2_4:20470759-20470778 Msa0320180:exon 15.0%
!! TCTTGATATATATATATCAT+GGG + chr2_4:20470760-20470779 Msa0320180:exon 15.0%
!!! AATTTTATACCCAAAAAAAA+AGG + chr2_4:20471216-20471235 Msa0320180:intron 15.0%
!!! ATTTAACTTCCTTTTTTTTT+GGG - chr2_4:20471228-20471247 None:intergenic 15.0%
!!! AATTTAACTTCCTTTTTTTT+TGG - chr2_4:20471229-20471248 None:intergenic 15.0%
!! AATAATTAAAGTTTATGATG+CGG + chr2_4:20471878-20471897 Msa0320180:three_prime_UTR 15.0%
!! ATATATATATCAAGATAACA+AGG - chr2_4:20470755-20470774 None:intergenic 15.0%
!! ATCTTGATATATATATATCA+TGG + chr2_4:20470759-20470778 Msa0320180:exon 15.0%
!! TCTTGATATATATATATCAT+GGG + chr2_4:20470760-20470779 Msa0320180:exon 15.0%
!!! AATTTTATACCCAAAAAAAA+AGG + chr2_4:20471216-20471235 Msa0320180:intron 15.0%
!!! ATTTAACTTCCTTTTTTTTT+GGG - chr2_4:20471228-20471247 None:intergenic 15.0%
!!! AATTTAACTTCCTTTTTTTT+TGG - chr2_4:20471229-20471248 None:intergenic 15.0%
!! AATAATTAAAGTTTATGATG+CGG + chr2_4:20471878-20471897 Msa0320180:three_prime_UTR 15.0%
!! AGTTAAATTCATTATACCAA+AGG + chr2_4:20471178-20471197 Msa0320180:intron 20.0%
!!! TAATTTAACTTTCTTTCCTT+TGG - chr2_4:20471197-20471216 None:intergenic 20.0%
!! AATAGTAATCCAATTCTATT+TGG + chr2_4:20471265-20471284 Msa0320180:intron 20.0%
!! TATTTATTGCATAATACAGA+TGG - chr2_4:20472040-20472059 None:intergenic 20.0%
!! AGTTAAATTCATTATACCAA+AGG + chr2_4:20471178-20471197 Msa0320180:intron 20.0%
!!! TAATTTAACTTTCTTTCCTT+TGG - chr2_4:20471197-20471216 None:intergenic 20.0%
!! AATAGTAATCCAATTCTATT+TGG + chr2_4:20471265-20471284 Msa0320180:intron 20.0%
!! TATTTATTGCATAATACAGA+TGG - chr2_4:20472040-20472059 None:intergenic 20.0%
! ATATGCAAGAAGTAAATGAA+AGG - chr2_4:20470728-20470747 None:intergenic 25.0%
! TTCAACAATTTCAACACTTT+GGG - chr2_4:20470893-20470912 None:intergenic 25.0%
! AATAGAATCTTGAGAAATGA+AGG + chr2_4:20471326-20471345 Msa0320180:intron 25.0%
! ATAATTATTAGACATAACCC+TGG - chr2_4:20471372-20471391 None:intergenic 25.0%
! AGGGTTATGTCTAATAATTA+TGG + chr2_4:20471371-20471390 Msa0320180:intron 25.0%
!!! CATCTGAATCATTTTTTTGA+AGG - chr2_4:20471398-20471417 None:intergenic 25.0%
! TTCAGATGATTAATGATACT+CGG + chr2_4:20471410-20471429 Msa0320180:intron 25.0%
! TATAATGGTTATATTGCAGA+TGG + chr2_4:20471513-20471532 Msa0320180:intron 25.0%
! TTTCACTGAAATCTTAACAA+TGG - chr2_4:20471688-20471707 None:intergenic 25.0%
! GTTTAATTGGCTAGTAATTA+GGG - chr2_4:20471815-20471834 None:intergenic 25.0%
! ATATGCAAGAAGTAAATGAA+AGG - chr2_4:20470728-20470747 None:intergenic 25.0%
! TTCAACAATTTCAACACTTT+GGG - chr2_4:20470893-20470912 None:intergenic 25.0%
! AATAGAATCTTGAGAAATGA+AGG + chr2_4:20471326-20471345 Msa0320180:intron 25.0%
! ATAATTATTAGACATAACCC+TGG - chr2_4:20471372-20471391 None:intergenic 25.0%
! AGGGTTATGTCTAATAATTA+TGG + chr2_4:20471371-20471390 Msa0320180:intron 25.0%
!!! CATCTGAATCATTTTTTTGA+AGG - chr2_4:20471398-20471417 None:intergenic 25.0%
! TTCAGATGATTAATGATACT+CGG + chr2_4:20471410-20471429 Msa0320180:intron 25.0%
! TATAATGGTTATATTGCAGA+TGG + chr2_4:20471513-20471532 Msa0320180:intron 25.0%
! TTTCACTGAAATCTTAACAA+TGG - chr2_4:20471688-20471707 None:intergenic 25.0%
! GTTTAATTGGCTAGTAATTA+GGG - chr2_4:20471815-20471834 None:intergenic 25.0%
CTTCAACAATTTCAACACTT+TGG - chr2_4:20470894-20470913 None:intergenic 30.0%
!! CAAAGTGTTGAAATTGTTGA+AGG + chr2_4:20470892-20470911 Msa0320180:CDS 30.0%
GTTGAAATTGTTGAAGGAAA+TGG + chr2_4:20470898-20470917 Msa0320180:CDS 30.0%
ATCTGAATTACCTTCAACAA+TGG - chr2_4:20470950-20470969 None:intergenic 30.0%
CATGACTATCCAAATAGAAT+TGG - chr2_4:20471277-20471296 None:intergenic 30.0%
! TAATTTGTGTAGCACAAAGT+TGG - chr2_4:20471544-20471563 None:intergenic 30.0%
! TTTGAGACAAAAATTGTTGC+TGG + chr2_4:20471648-20471667 Msa0320180:CDS 30.0%
GGTTTAATTGGCTAGTAATT+AGG - chr2_4:20471816-20471835 None:intergenic 30.0%
CCAAATTAAAGTCCTCAATA+GGG - chr2_4:20471837-20471856 None:intergenic 30.0%
! CCTATTGAGGACTTTAATTT+GGG + chr2_4:20471835-20471854 Msa0320180:three_prime_UTR 30.0%
AACAAAAACCTATGTTGTGT+TGG + chr2_4:20471999-20472018 Msa0320180:three_prime_UTR 30.0%
CTTCAACAATTTCAACACTT+TGG - chr2_4:20470894-20470913 None:intergenic 30.0%
!! CAAAGTGTTGAAATTGTTGA+AGG + chr2_4:20470892-20470911 Msa0320180:CDS 30.0%
GTTGAAATTGTTGAAGGAAA+TGG + chr2_4:20470898-20470917 Msa0320180:CDS 30.0%
ATCTGAATTACCTTCAACAA+TGG - chr2_4:20470950-20470969 None:intergenic 30.0%
CATGACTATCCAAATAGAAT+TGG - chr2_4:20471277-20471296 None:intergenic 30.0%
! TAATTTGTGTAGCACAAAGT+TGG - chr2_4:20471544-20471563 None:intergenic 30.0%
! TTTGAGACAAAAATTGTTGC+TGG + chr2_4:20471648-20471667 Msa0320180:CDS 30.0%
GGTTTAATTGGCTAGTAATT+AGG - chr2_4:20471816-20471835 None:intergenic 30.0%
CCAAATTAAAGTCCTCAATA+GGG - chr2_4:20471837-20471856 None:intergenic 30.0%
! CCTATTGAGGACTTTAATTT+GGG + chr2_4:20471835-20471854 Msa0320180:three_prime_UTR 30.0%
AACAAAAACCTATGTTGTGT+TGG + chr2_4:20471999-20472018 Msa0320180:three_prime_UTR 30.0%
GAAATTGTTGAAGGAAATGG+AGG + chr2_4:20470901-20470920 Msa0320180:CDS 35.0%
GATAGCTTCTTAATAGTTCC+GGG - chr2_4:20470928-20470947 None:intergenic 35.0%
GGATAGCTTCTTAATAGTTC+CGG - chr2_4:20470929-20470948 None:intergenic 35.0%
AAGAAGCTATCCATTGTTGA+AGG + chr2_4:20470937-20470956 Msa0320180:CDS 35.0%
! TTGTACCGACAAACTTTGAT+CGG - chr2_4:20471051-20471070 None:intergenic 35.0%
ATAATACATAAGCTCGTTCC+AGG + chr2_4:20471351-20471370 Msa0320180:intron 35.0%
TAATACATAAGCTCGTTCCA+GGG + chr2_4:20471352-20471371 Msa0320180:intron 35.0%
GCTACACAAATTAGATTCAG+TGG + chr2_4:20471551-20471570 Msa0320180:CDS 35.0%
CAAATTAGATTCAGTGGATG+AGG + chr2_4:20471557-20471576 Msa0320180:CDS 35.0%
TGGATATAACTACAGCTTAG+TGG + chr2_4:20471587-20471606 Msa0320180:CDS 35.0%
GGATATAACTACAGCTTAGT+GGG + chr2_4:20471588-20471607 Msa0320180:CDS 35.0%
TCAGTGAAATACCATACCAA+AGG + chr2_4:20471699-20471718 Msa0320180:CDS 35.0%
TAGCCAATTAAACCCTATTG+AGG + chr2_4:20471822-20471841 Msa0320180:exon 35.0%
AGTCCTCAATAGGGTTTAAT+TGG - chr2_4:20471828-20471847 None:intergenic 35.0%
CCCTATTGAGGACTTTAATT+TGG + chr2_4:20471834-20471853 Msa0320180:three_prime_UTR 35.0%
CCCAAATTAAAGTCCTCAAT+AGG - chr2_4:20471838-20471857 None:intergenic 35.0%
AGTGTGTTGAGTATACATCA+AGG + chr2_4:20471905-20471924 Msa0320180:three_prime_UTR 35.0%
TGAGTATACATCAAGGTCTT+TGG + chr2_4:20471912-20471931 Msa0320180:three_prime_UTR 35.0%
!!! TGAGGTTTGAGTGCTATTTT+GGG + chr2_4:20471968-20471987 Msa0320180:three_prime_UTR 35.0%
GAAATTGTTGAAGGAAATGG+AGG + chr2_4:20470901-20470920 Msa0320180:CDS 35.0%
GATAGCTTCTTAATAGTTCC+GGG - chr2_4:20470928-20470947 None:intergenic 35.0%
GGATAGCTTCTTAATAGTTC+CGG - chr2_4:20470929-20470948 None:intergenic 35.0%
AAGAAGCTATCCATTGTTGA+AGG + chr2_4:20470937-20470956 Msa0320180:CDS 35.0%
! TTGTACCGACAAACTTTGAT+CGG - chr2_4:20471051-20471070 None:intergenic 35.0%
ATAATACATAAGCTCGTTCC+AGG + chr2_4:20471351-20471370 Msa0320180:intron 35.0%
TAATACATAAGCTCGTTCCA+GGG + chr2_4:20471352-20471371 Msa0320180:intron 35.0%
GCTACACAAATTAGATTCAG+TGG + chr2_4:20471551-20471570 Msa0320180:CDS 35.0%
CAAATTAGATTCAGTGGATG+AGG + chr2_4:20471557-20471576 Msa0320180:CDS 35.0%
TGGATATAACTACAGCTTAG+TGG + chr2_4:20471587-20471606 Msa0320180:CDS 35.0%
GGATATAACTACAGCTTAGT+GGG + chr2_4:20471588-20471607 Msa0320180:CDS 35.0%
TCAGTGAAATACCATACCAA+AGG + chr2_4:20471699-20471718 Msa0320180:CDS 35.0%
TAGCCAATTAAACCCTATTG+AGG + chr2_4:20471822-20471841 Msa0320180:exon 35.0%
AGTCCTCAATAGGGTTTAAT+TGG - chr2_4:20471828-20471847 None:intergenic 35.0%
CCCTATTGAGGACTTTAATT+TGG + chr2_4:20471834-20471853 Msa0320180:three_prime_UTR 35.0%
CCCAAATTAAAGTCCTCAAT+AGG - chr2_4:20471838-20471857 None:intergenic 35.0%
AGTGTGTTGAGTATACATCA+AGG + chr2_4:20471905-20471924 Msa0320180:three_prime_UTR 35.0%
TGAGTATACATCAAGGTCTT+TGG + chr2_4:20471912-20471931 Msa0320180:three_prime_UTR 35.0%
!!! TGAGGTTTGAGTGCTATTTT+GGG + chr2_4:20471968-20471987 Msa0320180:three_prime_UTR 35.0%
GACAAACTTTGATCGGTTGT+CGG - chr2_4:20471044-20471063 None:intergenic 40.0%
GACAACCGATCAAAGTTTGT+CGG + chr2_4:20471043-20471062 Msa0320180:intron 40.0%
GACAAACATGATGCGACAAA+TGG + chr2_4:20471106-20471125 Msa0320180:intron 40.0%
TATAACTACAGCTTAGTGGG+AGG + chr2_4:20471591-20471610 Msa0320180:CDS 40.0%
!! AAAATTGTTGCTGGCTCTGA+TGG + chr2_4:20471657-20471676 Msa0320180:CDS 40.0%
AGATAGAGTTGCATCACCTT+TGG - chr2_4:20471718-20471737 None:intergenic 40.0%
CTTGATAAGTCCAGTTCCTT+TGG - chr2_4:20471766-20471785 None:intergenic 40.0%
CAAAGGAACTGGACTTATCA+AGG + chr2_4:20471764-20471783 Msa0320180:CDS 40.0%
!! TACATGTGTGTTGGCTTTGT+TGG + chr2_4:20471939-20471958 Msa0320180:three_prime_UTR 40.0%
!!! GTGAGGTTTGAGTGCTATTT+TGG + chr2_4:20471967-20471986 Msa0320180:three_prime_UTR 40.0%
CCTTATCACCAACACAACAT+AGG - chr2_4:20472010-20472029 None:intergenic 40.0%
!! CCTATGTTGTGTTGGTGATA+AGG + chr2_4:20472007-20472026 Msa0320180:three_prime_UTR 40.0%
GACAAACTTTGATCGGTTGT+CGG - chr2_4:20471044-20471063 None:intergenic 40.0%
GACAACCGATCAAAGTTTGT+CGG + chr2_4:20471043-20471062 Msa0320180:intron 40.0%
GACAAACATGATGCGACAAA+TGG + chr2_4:20471106-20471125 Msa0320180:intron 40.0%
TATAACTACAGCTTAGTGGG+AGG + chr2_4:20471591-20471610 Msa0320180:CDS 40.0%
!! AAAATTGTTGCTGGCTCTGA+TGG + chr2_4:20471657-20471676 Msa0320180:CDS 40.0%
AGATAGAGTTGCATCACCTT+TGG - chr2_4:20471718-20471737 None:intergenic 40.0%
CTTGATAAGTCCAGTTCCTT+TGG - chr2_4:20471766-20471785 None:intergenic 40.0%
CAAAGGAACTGGACTTATCA+AGG + chr2_4:20471764-20471783 Msa0320180:CDS 40.0%
!! TACATGTGTGTTGGCTTTGT+TGG + chr2_4:20471939-20471958 Msa0320180:three_prime_UTR 40.0%
!!! GTGAGGTTTGAGTGCTATTT+TGG + chr2_4:20471967-20471986 Msa0320180:three_prime_UTR 40.0%
CCTTATCACCAACACAACAT+AGG - chr2_4:20472010-20472029 None:intergenic 40.0%
!! CCTATGTTGTGTTGGTGATA+AGG + chr2_4:20472007-20472026 Msa0320180:three_prime_UTR 40.0%
TGATGAACATGTCTCAACCG+TGG + chr2_4:20470798-20470817 Msa0320180:CDS 45.0%
! AGAGCCTTGTAGAGTTTAGC+TGG - chr2_4:20470826-20470845 None:intergenic 45.0%
TGCTGATGAAATCGTCCCAA+AGG + chr2_4:20470855-20470874 Msa0320180:CDS 45.0%
TCAGTGGATGAGGCAAACTT+TGG + chr2_4:20471567-20471586 Msa0320180:CDS 45.0%
GAGTTGCATCACCTTTGGTA+TGG - chr2_4:20471713-20471732 None:intergenic 45.0%
! TCTATCTGAAGCAGTACGTG+AGG + chr2_4:20471731-20471750 Msa0320180:CDS 45.0%
TGGACTTATCAAGGCCATTG+AGG + chr2_4:20471773-20471792 Msa0320180:CDS 45.0%
GGACTTATCAAGGCCATTGA+GGG + chr2_4:20471774-20471793 Msa0320180:CDS 45.0%
TGCTAAAACGTAGCCCTCAA+TGG - chr2_4:20471790-20471809 None:intergenic 45.0%
! TTTGGCTCGTACATGTGTGT+TGG + chr2_4:20471930-20471949 Msa0320180:three_prime_UTR 45.0%
!! TGGCTTTGTTGGATGTTGTG+AGG + chr2_4:20471950-20471969 Msa0320180:three_prime_UTR 45.0%
TGATGAACATGTCTCAACCG+TGG + chr2_4:20470798-20470817 Msa0320180:CDS 45.0%
! AGAGCCTTGTAGAGTTTAGC+TGG - chr2_4:20470826-20470845 None:intergenic 45.0%
TGCTGATGAAATCGTCCCAA+AGG + chr2_4:20470855-20470874 Msa0320180:CDS 45.0%
TCAGTGGATGAGGCAAACTT+TGG + chr2_4:20471567-20471586 Msa0320180:CDS 45.0%
GAGTTGCATCACCTTTGGTA+TGG - chr2_4:20471713-20471732 None:intergenic 45.0%
! TCTATCTGAAGCAGTACGTG+AGG + chr2_4:20471731-20471750 Msa0320180:CDS 45.0%
TGGACTTATCAAGGCCATTG+AGG + chr2_4:20471773-20471792 Msa0320180:CDS 45.0%
GGACTTATCAAGGCCATTGA+GGG + chr2_4:20471774-20471793 Msa0320180:CDS 45.0%
TGCTAAAACGTAGCCCTCAA+TGG - chr2_4:20471790-20471809 None:intergenic 45.0%
! TTTGGCTCGTACATGTGTGT+TGG + chr2_4:20471930-20471949 Msa0320180:three_prime_UTR 45.0%
!! TGGCTTTGTTGGATGTTGTG+AGG + chr2_4:20471950-20471969 Msa0320180:three_prime_UTR 45.0%
!! ATTAAATTAAATTAAAATGA+CGG - chr2_4:20472088-20472107 None:intergenic 5.0%
!! ATTAAATTAAATTAAAATGA+CGG - chr2_4:20472088-20472107 None:intergenic 5.0%
GTAGAGTTTAGCTGGAGCCA+CGG - chr2_4:20470818-20470837 None:intergenic 50.0%
GGCTCCAGCTAAACTCTACA+AGG + chr2_4:20470819-20470838 Msa0320180:CDS 50.0%
GGGCAGCAGAAATCACCTTT+GGG - chr2_4:20470873-20470892 None:intergenic 50.0%
TGGGCAGCAGAAATCACCTT+TGG - chr2_4:20470874-20470893 None:intergenic 50.0%
GTTGAAGGAAATGGAGGACC+CGG + chr2_4:20470907-20470926 Msa0320180:CDS 50.0%
TACAGCTTAGTGGGAGGAAC+AGG + chr2_4:20471597-20471616 Msa0320180:CDS 50.0%
ACAGCTTAGTGGGAGGAACA+GGG + chr2_4:20471598-20471617 Msa0320180:CDS 50.0%
!! ATTGTTGCTGGCTCTGATGG+TGG + chr2_4:20471660-20471679 Msa0320180:CDS 50.0%
AGCAGTACGTGAGGAGACTA+AGG + chr2_4:20471740-20471759 Msa0320180:CDS 50.0%
GAGACTAAGGCCAAAGGAAC+TGG + chr2_4:20471753-20471772 Msa0320180:CDS 50.0%
GTAGAGTTTAGCTGGAGCCA+CGG - chr2_4:20470818-20470837 None:intergenic 50.0%
GGCTCCAGCTAAACTCTACA+AGG + chr2_4:20470819-20470838 Msa0320180:CDS 50.0%
GGGCAGCAGAAATCACCTTT+GGG - chr2_4:20470873-20470892 None:intergenic 50.0%
TGGGCAGCAGAAATCACCTT+TGG - chr2_4:20470874-20470893 None:intergenic 50.0%
GTTGAAGGAAATGGAGGACC+CGG + chr2_4:20470907-20470926 Msa0320180:CDS 50.0%
TACAGCTTAGTGGGAGGAAC+AGG + chr2_4:20471597-20471616 Msa0320180:CDS 50.0%
ACAGCTTAGTGGGAGGAACA+GGG + chr2_4:20471598-20471617 Msa0320180:CDS 50.0%
!! ATTGTTGCTGGCTCTGATGG+TGG + chr2_4:20471660-20471679 Msa0320180:CDS 50.0%
AGCAGTACGTGAGGAGACTA+AGG + chr2_4:20471740-20471759 Msa0320180:CDS 50.0%
GAGACTAAGGCCAAAGGAAC+TGG + chr2_4:20471753-20471772 Msa0320180:CDS 50.0%
CTTAGTGGGAGGAACAGGGT+TGG + chr2_4:20471602-20471621 Msa0320180:CDS 55.0%
CGTGAGGAGACTAAGGCCAA+AGG + chr2_4:20471747-20471766 Msa0320180:CDS 55.0%
CTTAGTGGGAGGAACAGGGT+TGG + chr2_4:20471602-20471621 Msa0320180:CDS 55.0%
CGTGAGGAGACTAAGGCCAA+AGG + chr2_4:20471747-20471766 Msa0320180:CDS 55.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 20470679 20472123 20470679 ID=Msa0320180;Name=Msa0320180
chr2_4 mRNA 20470679 20472123 20470679 ID=Msa0320180-mRNA-1;Parent=Msa0320180;Name=Msa0320180-mRNA-1;_AED=0.35;_eAED=0.35;_QI=99|1|1|1|1|1|2|299|157
chr2_4 exon 20470679 20470958 20470679 ID=Msa0320180-mRNA-1:exon:7529;Parent=Msa0320180-mRNA-1
chr2_4 exon 20471532 20472123 20471532 ID=Msa0320180-mRNA-1:exon:7530;Parent=Msa0320180-mRNA-1
chr2_4 five_prime_UTR 20470679 20470777 20470679 ID=Msa0320180-mRNA-1:five_prime_utr;Parent=Msa0320180-mRNA-1
chr2_4 CDS 20470778 20470958 20470778 ID=Msa0320180-mRNA-1:cds;Parent=Msa0320180-mRNA-1
chr2_4 CDS 20471532 20471824 20471532 ID=Msa0320180-mRNA-1:cds;Parent=Msa0320180-mRNA-1
chr2_4 three_prime_UTR 20471825 20472123 20471825 ID=Msa0320180-mRNA-1:three_prime_utr;Parent=Msa0320180-mRNA-1
Gene Sequence

>Msa0320180

ATGGGTGTTTTTACTTTCAATGATGAACATGTCTCAACCGTGGCTCCAGCTAAACTCTACAAGGCTCTTGCAAAAGATGCTGATGAAATCGTCCCAAAGGTGATTTCTGCTGCCCAAAGTGTTGAAATTGTTGAAGGAAATGGAGGACCCGGAACTATTAAGAAGCTATCCATTGTTGAAGATGGCAAAACCAACTTTGTGCTACACAAATTAGATTCAGTGGATGAGGCAAACTTTGGATATAACTACAGCTTAGTGGGAGGAACAGGGTTGGATGAAAGTTTAGAGAAAGTTGAATTTGAGACAAAAATTGTTGCTGGCTCTGATGGTGGATCCATTGTTAAGATTTCAGTGAAATACCATACCAAAGGTGATGCAACTCTATCTGAAGCAGTACGTGAGGAGACTAAGGCCAAAGGAACTGGACTTATCAAGGCCATTGAGGGCTACGTTTTAGCAAACCCTAATTACTAG
Protein sequence

>Msa0320180

MGVFTFNDEHVSTVAPAKLYKALAKDADEIVPKVISAAQSVEIVEGNGGPGTIKKLSIVEDGKTNFVLHKLDSVDEANFGYNYSLVGGTGLDESLEKVEFETKIVAGSDGGSIVKISVKYHTKGDATLSEAVREETKAKGTGLIKAIEGYVLANPNY*