AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0646670


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0646670 MtrunA17_Chr4g0034301 99.375 160 1 0 1 160 1 160 1.12e-115 324
Msa0646670 MtrunA17_Chr2g0305551 81.333 150 28 0 8 157 13 162 2.59e-87 253
Msa0646670 MtrunA17_Chr2g0305541 81.879 149 27 0 9 157 9 157 5.26e-87 252
Msa0646670 MtrunA17_Chr4g0034311 83.221 149 25 0 9 157 185 333 2.15e-85 254
Msa0646670 MtrunA17_Chr4g0034311 79.487 156 29 1 2 157 10 162 9.10e-84 250
Msa0646670 MtrunA17_Chr1g0197501 48.000 150 77 1 8 156 22 171 2.49e-38 128
Msa0646670 MtrunA17_Chr5g0417631 47.651 149 74 2 8 156 2 146 6.74e-37 124
Msa0646670 MtrunA17_Chr5g0435991 45.638 149 77 2 8 156 2 146 1.57e-36 123
Msa0646670 MtrunA17_Chr5g0435621 45.638 149 77 2 8 156 2 146 3.37e-36 122
Msa0646670 MtrunA17_Chr1g0148751 47.651 149 73 3 8 156 2 145 2.53e-35 120
Msa0646670 MtrunA17_Chr5g0435981 46.309 149 75 3 8 156 2 145 3.15e-34 117
Msa0646670 MtrunA17_Chr5g0427351 45.270 148 77 2 9 156 3 146 6.57e-34 116
Msa0646670 MtrunA17_Chr5g0435611 44.000 150 80 2 8 157 2 147 3.41e-33 115
Msa0646670 MtrunA17_Chr1g0197531 44.295 149 80 1 8 156 2 147 5.68e-33 114
Msa0646670 MtrunA17_Chr1g0170851 41.892 148 83 1 9 156 3 147 8.18e-31 108
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0646670 AT2G16060.1 79.605 152 31 0 9 160 9 160 1.93e-88 255
Msa0646670 AT3G10520.1 52.667 150 71 0 8 157 5 154 6.93e-51 160

Find 32 sgRNAs with CRISPR-Local

Find 116 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACACTTACCCTTCAGAATA+TGG 0.210157 4_3:+52450724 Msa0646670:exon
CTACTACACCATATTTAAAA+TGG 0.249717 4_3:-52451594 None:intergenic
GGTGACAAAGTTTGCACTAT+TGG 0.325692 4_3:+52451788 Msa0646670:intron
AAAAGATTCAAAAGTTCCTT+TGG 0.334733 4_3:+52451001 Msa0646670:CDS
TTCTCTGACCGTGACTTTAC+CGG 0.339739 4_3:-52451545 None:intergenic
CCTTCAGAATATGGGCACTT+TGG 0.384904 4_3:+52450733 Msa0646670:exon
TATAAACTAGAGAGTTTAAG+AGG 0.396192 4_3:-52451919 None:intergenic
AAGAAGAATTCTGCAGAGTT+AGG 0.397154 4_3:+52450815 Msa0646670:CDS
AACTAGAGAGTTTAAGAGGA+AGG 0.427518 4_3:-52451915 None:intergenic
ATAGCCGGTGACCACATTTC+AGG 0.433187 4_3:-52451831 None:intergenic
TAAGTGTTACGTGCTAATTA+AGG 0.453125 4_3:-52450709 None:intergenic
GCAGTTCAATTGCGGAAATC+CGG 0.465498 4_3:+52451526 Msa0646670:CDS
ATGGGCACTTTGGATACAAA+AGG 0.471419 4_3:+52450743 Msa0646670:CDS
CCCCATGCATTCTTCATAGC+CGG 0.478259 4_3:-52451846 None:intergenic
TAATTAAGGTATGTGATTTG+TGG 0.489143 4_3:-52450695 None:intergenic
TGCCATGTCTGTCTTTCTCA+TGG 0.508395 4_3:+52451049 Msa0646670:CDS
ACACTTACCCTTCAGAATAT+GGG 0.519885 4_3:+52450725 Msa0646670:exon
CACCGGCTATGAAGAATGCA+TGG 0.521161 4_3:+52451844 Msa0646670:CDS
GGGAGAAGCTTATGATCAGC+TGG 0.524272 4_3:+52451866 Msa0646670:CDS
AAGCTCTTGTGGTGAAGTCA+TGG 0.525782 4_3:+52450783 Msa0646670:CDS
CCAAAGTGCCCATATTCTGA+AGG 0.558548 4_3:-52450733 None:intergenic
ACCGGCTATGAAGAATGCAT+GGG 0.592597 4_3:+52451845 Msa0646670:CDS
CAAAGTGCCCATATTCTGAA+GGG 0.600691 4_3:-52450732 None:intergenic
AGTACCTGAAATGTGGTCAC+CGG 0.611768 4_3:+52451827 Msa0646670:CDS
GTGAATCAGCAGTTCAATTG+CGG 0.612574 4_3:+52451518 Msa0646670:CDS
CATGGCATGAGGCTTGAGCT+TGG 0.612875 4_3:-52451033 None:intergenic
TGAAGAACAAGAAGCTCTTG+TGG 0.616139 4_3:+52450772 Msa0646670:CDS
AAGAAGCAGTACCTGAAATG+TGG 0.626355 4_3:+52451820 Msa0646670:CDS
GCGGAAATCCGGTAAAGTCA+CGG 0.634999 4_3:+52451537 Msa0646670:CDS
AGAAAGACAGACATGGCATG+AGG 0.665594 4_3:-52451044 None:intergenic
CCGGCTATGAAGAATGCATG+GGG 0.704265 4_3:+52451846 Msa0646670:CDS
TACCATGAGAAAGACAGACA+TGG 0.720993 4_3:-52451051 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAAGTATATTTTTTTTACT+AGG + chr4_3:52451647-52451666 Msa0646670:intron 10.0%
!!! TAAGTATATTTTTTTTACTA+GGG + chr4_3:52451648-52451667 Msa0646670:intron 10.0%
!!! TTTAATTTTTTGATTTTGTA+AGG + chr4_3:52451767-52451786 Msa0646670:intron 10.0%
!!! ATAAGTATATTTTTTTTACT+AGG + chr4_3:52451647-52451666 Msa0646670:intron 10.0%
!!! TAAGTATATTTTTTTTACTA+GGG + chr4_3:52451648-52451667 Msa0646670:intron 10.0%
!!! TTTAATTTTTTGATTTTGTA+AGG + chr4_3:52451767-52451786 Msa0646670:intron 10.0%
!! TTACATTAAAATTTGACATA+AGG + chr4_3:52451703-52451722 Msa0646670:intron 15.0%
!! TTACATTAAAATTTGACATA+AGG + chr4_3:52451703-52451722 Msa0646670:intron 15.0%
!!! CTAATTCAGTAACTAAATTT+TGG + chr4_3:52451136-52451155 Msa0646670:intron 20.0%
!! TATCATTTCTCATATTCAAA+TGG - chr4_3:52451274-52451293 None:intergenic 20.0%
!! TTAAAATTTGACATAAGGTT+TGG + chr4_3:52451708-52451727 Msa0646670:intron 20.0%
!!! CTAATTCAGTAACTAAATTT+TGG + chr4_3:52451136-52451155 Msa0646670:intron 20.0%
!! TATCATTTCTCATATTCAAA+TGG - chr4_3:52451274-52451293 None:intergenic 20.0%
!! TTAAAATTTGACATAAGGTT+TGG + chr4_3:52451708-52451727 Msa0646670:intron 20.0%
! TAATTAAGGTATGTGATTTG+TGG - chr4_3:52450698-52450717 None:intergenic 25.0%
!!! TTTTTTTGAAGTAAGTAGCT+AGG + chr4_3:52450846-52450865 Msa0646670:intron 25.0%
!!! TTCAACATTTTTTACTCAAG+AGG + chr4_3:52450875-52450894 Msa0646670:intron 25.0%
! AAAAGATTCAAAAGTTCCTT+TGG + chr4_3:52451001-52451020 Msa0646670:CDS 25.0%
! TTTAGTTACTGAATTAGAGA+CGG - chr4_3:52451133-52451152 None:intergenic 25.0%
! AAAACAATAGAGAGAGAAAA+AGG - chr4_3:52451373-52451392 None:intergenic 25.0%
!!! TTGTTTTTCTCACTTCTTAT+TGG - chr4_3:52451399-52451418 None:intergenic 25.0%
! AATAAGAAGTGAGAAAAACA+AGG + chr4_3:52451398-52451417 Msa0646670:intron 25.0%
! GCAATAACAAAATAATTAGC+TGG - chr4_3:52451492-52451511 None:intergenic 25.0%
! GAATCAAGCTTGAAAAAATT+AGG + chr4_3:52451565-52451584 Msa0646670:CDS 25.0%
! CTACTACACCATATTTAAAA+TGG - chr4_3:52451597-52451616 None:intergenic 25.0%
! TAATTAAGGTATGTGATTTG+TGG - chr4_3:52450698-52450717 None:intergenic 25.0%
!!! TTTTTTTGAAGTAAGTAGCT+AGG + chr4_3:52450846-52450865 Msa0646670:intron 25.0%
!!! TTCAACATTTTTTACTCAAG+AGG + chr4_3:52450875-52450894 Msa0646670:intron 25.0%
! AAAAGATTCAAAAGTTCCTT+TGG + chr4_3:52451001-52451020 Msa0646670:CDS 25.0%
! TTTAGTTACTGAATTAGAGA+CGG - chr4_3:52451133-52451152 None:intergenic 25.0%
! AAAACAATAGAGAGAGAAAA+AGG - chr4_3:52451373-52451392 None:intergenic 25.0%
!!! TTGTTTTTCTCACTTCTTAT+TGG - chr4_3:52451399-52451418 None:intergenic 25.0%
! AATAAGAAGTGAGAAAAACA+AGG + chr4_3:52451398-52451417 Msa0646670:intron 25.0%
! GCAATAACAAAATAATTAGC+TGG - chr4_3:52451492-52451511 None:intergenic 25.0%
! GAATCAAGCTTGAAAAAATT+AGG + chr4_3:52451565-52451584 Msa0646670:CDS 25.0%
! CTACTACACCATATTTAAAA+TGG - chr4_3:52451597-52451616 None:intergenic 25.0%
TAAGTGTTACGTGCTAATTA+AGG - chr4_3:52450712-52450731 None:intergenic 30.0%
GTCAAAAAGTTGTCAAAAAG+TGG - chr4_3:52451306-52451325 None:intergenic 30.0%
TCAACAAAGTTGTCATCAAA+TGG + chr4_3:52451424-52451443 Msa0646670:intron 30.0%
! GGTGCTAACCATTTTAAATA+TGG + chr4_3:52451586-52451605 Msa0646670:CDS 30.0%
TAAGTGTTACGTGCTAATTA+AGG - chr4_3:52450712-52450731 None:intergenic 30.0%
GTCAAAAAGTTGTCAAAAAG+TGG - chr4_3:52451306-52451325 None:intergenic 30.0%
TCAACAAAGTTGTCATCAAA+TGG + chr4_3:52451424-52451443 Msa0646670:intron 30.0%
! GGTGCTAACCATTTTAAATA+TGG + chr4_3:52451586-52451605 Msa0646670:CDS 30.0%
AACACTTACCCTTCAGAATA+TGG + chr4_3:52450724-52450743 Msa0646670:exon 35.0%
ACACTTACCCTTCAGAATAT+GGG + chr4_3:52450725-52450744 Msa0646670:exon 35.0%
AAGAAGAATTCTGCAGAGTT+AGG + chr4_3:52450815-52450834 Msa0646670:CDS 35.0%
AAATCCGTCTCTAATGTACT+CGG - chr4_3:52451163-52451182 None:intergenic 35.0%
TGACAAAGTCAAAGCTTGAA+TGG - chr4_3:52451237-52451256 None:intergenic 35.0%
TCTCATATTCAAATGGTGAG+TGG - chr4_3:52451267-52451286 None:intergenic 35.0%
!! TTCAGGTACTGCTTCTTTTA+TGG - chr4_3:52451817-52451836 None:intergenic 35.0%
AACACTTACCCTTCAGAATA+TGG + chr4_3:52450724-52450743 Msa0646670:exon 35.0%
ACACTTACCCTTCAGAATAT+GGG + chr4_3:52450725-52450744 Msa0646670:exon 35.0%
AAGAAGAATTCTGCAGAGTT+AGG + chr4_3:52450815-52450834 Msa0646670:CDS 35.0%
AAATCCGTCTCTAATGTACT+CGG - chr4_3:52451163-52451182 None:intergenic 35.0%
TGACAAAGTCAAAGCTTGAA+TGG - chr4_3:52451237-52451256 None:intergenic 35.0%
TCTCATATTCAAATGGTGAG+TGG - chr4_3:52451267-52451286 None:intergenic 35.0%
!! TTCAGGTACTGCTTCTTTTA+TGG - chr4_3:52451817-52451836 None:intergenic 35.0%
! CAAAGTGCCCATATTCTGAA+GGG - chr4_3:52450735-52450754 None:intergenic 40.0%
ATGGGCACTTTGGATACAAA+AGG + chr4_3:52450743-52450762 Msa0646670:CDS 40.0%
TGAAGAACAAGAAGCTCTTG+TGG + chr4_3:52450772-52450791 Msa0646670:CDS 40.0%
!! GAGAACAGTTTTTGAGCTGA+TGG - chr4_3:52450975-52450994 None:intergenic 40.0%
TACCATGAGAAAGACAGACA+TGG - chr4_3:52451054-52451073 None:intergenic 40.0%
AGAGAGAAAAAGGAAGAGAG+AGG - chr4_3:52451363-52451382 None:intergenic 40.0%
GTGAATCAGCAGTTCAATTG+CGG + chr4_3:52451518-52451537 Msa0646670:CDS 40.0%
! TGTAGTAGACGAGCATTTTG+AGG + chr4_3:52451609-52451628 Msa0646670:CDS 40.0%
GGTGACAAAGTTTGCACTAT+TGG + chr4_3:52451788-52451807 Msa0646670:intron 40.0%
AAGAAGCAGTACCTGAAATG+TGG + chr4_3:52451820-52451839 Msa0646670:CDS 40.0%
! CAAAGTGCCCATATTCTGAA+GGG - chr4_3:52450735-52450754 None:intergenic 40.0%
ATGGGCACTTTGGATACAAA+AGG + chr4_3:52450743-52450762 Msa0646670:CDS 40.0%
TGAAGAACAAGAAGCTCTTG+TGG + chr4_3:52450772-52450791 Msa0646670:CDS 40.0%
!! GAGAACAGTTTTTGAGCTGA+TGG - chr4_3:52450975-52450994 None:intergenic 40.0%
TACCATGAGAAAGACAGACA+TGG - chr4_3:52451054-52451073 None:intergenic 40.0%
AGAGAGAAAAAGGAAGAGAG+AGG - chr4_3:52451363-52451382 None:intergenic 40.0%
GTGAATCAGCAGTTCAATTG+CGG + chr4_3:52451518-52451537 Msa0646670:CDS 40.0%
! TGTAGTAGACGAGCATTTTG+AGG + chr4_3:52451609-52451628 Msa0646670:CDS 40.0%
GGTGACAAAGTTTGCACTAT+TGG + chr4_3:52451788-52451807 Msa0646670:intron 40.0%
AAGAAGCAGTACCTGAAATG+TGG + chr4_3:52451820-52451839 Msa0646670:CDS 40.0%
! CCAAAGTGCCCATATTCTGA+AGG - chr4_3:52450736-52450755 None:intergenic 45.0%
CCTTCAGAATATGGGCACTT+TGG + chr4_3:52450733-52450752 Msa0646670:exon 45.0%
AAGCTCTTGTGGTGAAGTCA+TGG + chr4_3:52450783-52450802 Msa0646670:CDS 45.0%
!!! AGCTTGGTGTTTTGCTCCAA+AGG - chr4_3:52451020-52451039 None:intergenic 45.0%
AGAAAGACAGACATGGCATG+AGG - chr4_3:52451047-52451066 None:intergenic 45.0%
TGCCATGTCTGTCTTTCTCA+TGG + chr4_3:52451049-52451068 Msa0646670:CDS 45.0%
TGGACCGAGTACATTAGAGA+CGG + chr4_3:52451156-52451175 Msa0646670:intron 45.0%
AGTCAAAGCTTGAATGGTCG+TGG - chr4_3:52451231-52451250 None:intergenic 45.0%
GCAGTTCAATTGCGGAAATC+CGG + chr4_3:52451526-52451545 Msa0646670:CDS 45.0%
TTCTCTGACCGTGACTTTAC+CGG - chr4_3:52451548-52451567 None:intergenic 45.0%
AGTACCTGAAATGTGGTCAC+CGG + chr4_3:52451827-52451846 Msa0646670:CDS 45.0%
ACCGGCTATGAAGAATGCAT+GGG + chr4_3:52451845-52451864 Msa0646670:CDS 45.0%
! CCAAAGTGCCCATATTCTGA+AGG - chr4_3:52450736-52450755 None:intergenic 45.0%
CCTTCAGAATATGGGCACTT+TGG + chr4_3:52450733-52450752 Msa0646670:exon 45.0%
AAGCTCTTGTGGTGAAGTCA+TGG + chr4_3:52450783-52450802 Msa0646670:CDS 45.0%
!!! AGCTTGGTGTTTTGCTCCAA+AGG - chr4_3:52451020-52451039 None:intergenic 45.0%
AGAAAGACAGACATGGCATG+AGG - chr4_3:52451047-52451066 None:intergenic 45.0%
TGCCATGTCTGTCTTTCTCA+TGG + chr4_3:52451049-52451068 Msa0646670:CDS 45.0%
TGGACCGAGTACATTAGAGA+CGG + chr4_3:52451156-52451175 Msa0646670:intron 45.0%
AGTCAAAGCTTGAATGGTCG+TGG - chr4_3:52451231-52451250 None:intergenic 45.0%
GCAGTTCAATTGCGGAAATC+CGG + chr4_3:52451526-52451545 Msa0646670:CDS 45.0%
TTCTCTGACCGTGACTTTAC+CGG - chr4_3:52451548-52451567 None:intergenic 45.0%
AGTACCTGAAATGTGGTCAC+CGG + chr4_3:52451827-52451846 Msa0646670:CDS 45.0%
ACCGGCTATGAAGAATGCAT+GGG + chr4_3:52451845-52451864 Msa0646670:CDS 45.0%
GCGGAAATCCGGTAAAGTCA+CGG + chr4_3:52451537-52451556 Msa0646670:CDS 50.0%
ATAGCCGGTGACCACATTTC+AGG - chr4_3:52451834-52451853 None:intergenic 50.0%
! CACCGGCTATGAAGAATGCA+TGG + chr4_3:52451844-52451863 Msa0646670:CDS 50.0%
CCCCATGCATTCTTCATAGC+CGG - chr4_3:52451849-52451868 None:intergenic 50.0%
CCGGCTATGAAGAATGCATG+GGG + chr4_3:52451846-52451865 Msa0646670:CDS 50.0%
GGGAGAAGCTTATGATCAGC+TGG + chr4_3:52451866-52451885 Msa0646670:CDS 50.0%
GCGGAAATCCGGTAAAGTCA+CGG + chr4_3:52451537-52451556 Msa0646670:CDS 50.0%
ATAGCCGGTGACCACATTTC+AGG - chr4_3:52451834-52451853 None:intergenic 50.0%
! CACCGGCTATGAAGAATGCA+TGG + chr4_3:52451844-52451863 Msa0646670:CDS 50.0%
CCCCATGCATTCTTCATAGC+CGG - chr4_3:52451849-52451868 None:intergenic 50.0%
CCGGCTATGAAGAATGCATG+GGG + chr4_3:52451846-52451865 Msa0646670:CDS 50.0%
GGGAGAAGCTTATGATCAGC+TGG + chr4_3:52451866-52451885 Msa0646670:CDS 50.0%
CATGGCATGAGGCTTGAGCT+TGG - chr4_3:52451036-52451055 None:intergenic 55.0%
CATGGCATGAGGCTTGAGCT+TGG - chr4_3:52451036-52451055 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 52450688 52451926 52450688 ID=Msa0646670;Name=Msa0646670
chr4_3 mRNA 52450688 52451926 52450688 ID=Msa0646670-mRNA-1;Parent=Msa0646670;Name=Msa0646670-mRNA-1;_AED=0.12;_eAED=0.12;_QI=55|1|0.75|1|1|1|4|0|160
chr4_3 exon 52450688 52450855 52450688 ID=Msa0646670-mRNA-1:exon:12862;Parent=Msa0646670-mRNA-1
chr4_3 exon 52450956 52451070 52450956 ID=Msa0646670-mRNA-1:exon:12863;Parent=Msa0646670-mRNA-1
chr4_3 exon 52451514 52451630 52451514 ID=Msa0646670-mRNA-1:exon:12864;Parent=Msa0646670-mRNA-1
chr4_3 exon 52451789 52451926 52451789 ID=Msa0646670-mRNA-1:exon:12865;Parent=Msa0646670-mRNA-1
chr4_3 five_prime_UTR 52450688 52450742 52450688 ID=Msa0646670-mRNA-1:five_prime_utr;Parent=Msa0646670-mRNA-1
chr4_3 CDS 52450743 52450855 52450743 ID=Msa0646670-mRNA-1:cds;Parent=Msa0646670-mRNA-1
chr4_3 CDS 52450956 52451070 52450956 ID=Msa0646670-mRNA-1:cds;Parent=Msa0646670-mRNA-1
chr4_3 CDS 52451514 52451630 52451514 ID=Msa0646670-mRNA-1:cds;Parent=Msa0646670-mRNA-1
chr4_3 CDS 52451789 52451926 52451789 ID=Msa0646670-mRNA-1:cds;Parent=Msa0646670-mRNA-1
Gene Sequence

>Msa0646670

ATGGGCACTTTGGATACAAAAGGTTTCACTGAAGAACAAGAAGCTCTTGTGGTGAAGTCATGGAATGCAATGAAGAAGAATTCTGCAGAGTTAGGACTAAAGCTTTTTTTGAAAATATTTGAGATTGCTCCATCAGCTCAAAAACTGTTCTCATTCTTAAAAGATTCAAAAGTTCCTTTGGAGCAAAACACCAAGCTCAAGCCTCATGCCATGTCTGTCTTTCTCATGACATGTGAATCAGCAGTTCAATTGCGGAAATCCGGTAAAGTCACGGTCAGAGAATCAAGCTTGAAAAAATTAGGTGCTAACCATTTTAAATATGGTGTAGTAGACGAGCATTTTGAGGTGACAAAGTTTGCACTATTGGAGACCATAAAAGAAGCAGTACCTGAAATGTGGTCACCGGCTATGAAGAATGCATGGGGAGAAGCTTATGATCAGCTGGTCAATGCTATCAAATCTGAAATGAAACCTTCCTCTTAA

Protein sequence

>Msa0646670

MGTLDTKGFTEEQEALVVKSWNAMKKNSAELGLKLFLKIFEIAPSAQKLFSFLKDSKVPLEQNTKLKPHAMSVFLMTCESAVQLRKSGKVTVRESSLKKLGANHFKYGVVDEHFEVTKFALLETIKEAVPEMWSPAMKNAWGEAYDQLVNAIKSEMKPSS*