AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0605020


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0605020 MtrunA17_Chr4g0034301 99.375 160 1 0 1 160 1 160 1.12e-115 324
Msa0605020 MtrunA17_Chr2g0305551 81.333 150 28 0 8 157 13 162 2.59e-87 253
Msa0605020 MtrunA17_Chr2g0305541 81.879 149 27 0 9 157 9 157 5.26e-87 252
Msa0605020 MtrunA17_Chr4g0034311 83.221 149 25 0 9 157 185 333 2.15e-85 254
Msa0605020 MtrunA17_Chr4g0034311 79.487 156 29 1 2 157 10 162 9.10e-84 250
Msa0605020 MtrunA17_Chr1g0197501 48.000 150 77 1 8 156 22 171 2.49e-38 128
Msa0605020 MtrunA17_Chr5g0417631 47.651 149 74 2 8 156 2 146 6.74e-37 124
Msa0605020 MtrunA17_Chr5g0435991 45.638 149 77 2 8 156 2 146 1.57e-36 123
Msa0605020 MtrunA17_Chr5g0435621 45.638 149 77 2 8 156 2 146 3.37e-36 122
Msa0605020 MtrunA17_Chr1g0148751 47.651 149 73 3 8 156 2 145 2.53e-35 120
Msa0605020 MtrunA17_Chr5g0435981 46.309 149 75 3 8 156 2 145 3.15e-34 117
Msa0605020 MtrunA17_Chr5g0427351 45.270 148 77 2 9 156 3 146 6.57e-34 116
Msa0605020 MtrunA17_Chr5g0435611 44.000 150 80 2 8 157 2 147 3.41e-33 115
Msa0605020 MtrunA17_Chr1g0197531 44.295 149 80 1 8 156 2 147 5.68e-33 114
Msa0605020 MtrunA17_Chr1g0170851 41.892 148 83 1 9 156 3 147 8.18e-31 108
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0605020 AT2G16060.1 79.605 152 31 0 9 160 9 160 1.93e-88 255
Msa0605020 AT3G10520.1 52.667 150 71 0 8 157 5 154 6.93e-51 160

Find 32 sgRNAs with CRISPR-Local

Find 118 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACACTTACCCTTCAGAATA+TGG 0.210157 4_2:-50788642 Msa0605020:exon
CTACTACACCATATTTAAAA+TGG 0.249717 4_2:+50787783 None:intergenic
GGTGACAAAGTTTGCACTAT+TGG 0.325692 4_2:-50787589 Msa0605020:CDS
AAAAGATTCAAAAGTTCCTT+TGG 0.334733 4_2:-50788365 Msa0605020:CDS
TTCTCTGACCGTGACTTTAC+CGG 0.339739 4_2:+50787832 None:intergenic
CCTTCAGAATATGGGCACTT+TGG 0.384904 4_2:-50788633 Msa0605020:exon
TATAAACTAGAGAGTTTAAG+AGG 0.396192 4_2:+50787458 None:intergenic
AAGAAGAATTCTGCAGAGTT+AGG 0.397154 4_2:-50788551 Msa0605020:CDS
AACTAGAGAGTTTAAGAGGA+AGG 0.427518 4_2:+50787462 None:intergenic
ATAGCCGGTGACCACATTTC+AGG 0.433187 4_2:+50787546 None:intergenic
TAAGTGTTACGTGCTAATTA+AGG 0.453125 4_2:+50788657 None:intergenic
GCAGTTCAATTGCGGAAATC+CGG 0.465498 4_2:-50787851 Msa0605020:CDS
ATGGGCACTTTGGATACAAA+AGG 0.471419 4_2:-50788623 Msa0605020:CDS
CCCCATGCATTCTTCATAGC+CGG 0.478259 4_2:+50787531 None:intergenic
TAATTAAGGTATGTGATTTG+TGG 0.489143 4_2:+50788671 None:intergenic
TGCCATGTCTGTCTTTCTCA+TGG 0.508395 4_2:-50788317 Msa0605020:intron
ACACTTACCCTTCAGAATAT+GGG 0.519885 4_2:-50788641 Msa0605020:exon
CACCGGCTATGAAGAATGCA+TGG 0.521161 4_2:-50787533 Msa0605020:CDS
GGGAGAAGCTTATGATCAGC+TGG 0.524272 4_2:-50787511 Msa0605020:CDS
AAGCTCTTGTGGTGAAGTCA+TGG 0.525782 4_2:-50788583 Msa0605020:CDS
CCAAAGTGCCCATATTCTGA+AGG 0.558548 4_2:+50788633 None:intergenic
ACCGGCTATGAAGAATGCAT+GGG 0.592597 4_2:-50787532 Msa0605020:CDS
CAAAGTGCCCATATTCTGAA+GGG 0.600691 4_2:+50788634 None:intergenic
AGTACCTGAAATGTGGTCAC+CGG 0.611768 4_2:-50787550 Msa0605020:CDS
GTGAATCAGCAGTTCAATTG+CGG 0.612574 4_2:-50787859 Msa0605020:CDS
CATGGCATGAGGCTTGAGCT+TGG 0.612875 4_2:+50788333 None:intergenic
TGAAGAACAAGAAGCTCTTG+TGG 0.616139 4_2:-50788594 Msa0605020:CDS
AAGAAGCAGTACCTGAAATG+TGG 0.626355 4_2:-50787557 Msa0605020:CDS
GCGGAAATCCGGTAAAGTCA+CGG 0.634999 4_2:-50787840 Msa0605020:CDS
AGAAAGACAGACATGGCATG+AGG 0.665594 4_2:+50788322 None:intergenic
CCGGCTATGAAGAATGCATG+GGG 0.704265 4_2:-50787531 Msa0605020:CDS
TACCATGAGAAAGACAGACA+TGG 0.720993 4_2:+50788315 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAAGTATATTTTTTTTACT+AGG - chr4_2:50788421-50788440 Msa0605020:intron 10.0%
!!! TAAGTATATTTTTTTTACTA+GGG - chr4_2:50788422-50788441 Msa0605020:intron 10.0%
!!! TTTAATTTTTTGATTTTGTA+AGG - chr4_2:50788541-50788560 Msa0605020:CDS 10.0%
!!! ATAAGTATATTTTTTTTACT+AGG - chr4_2:50788421-50788440 Msa0605020:intron 10.0%
!!! TAAGTATATTTTTTTTACTA+GGG - chr4_2:50788422-50788441 Msa0605020:intron 10.0%
!!! TTTAATTTTTTGATTTTGTA+AGG - chr4_2:50788541-50788560 Msa0605020:CDS 10.0%
!! TTACATTAAAATTTGACATA+AGG - chr4_2:50788477-50788496 Msa0605020:intron 15.0%
!! TTACATTAAAATTTGACATA+AGG - chr4_2:50788477-50788496 Msa0605020:intron 15.0%
!!! CTAATTCAGTAACTAAATTT+TGG - chr4_2:50787921-50787940 Msa0605020:intron 20.0%
!! TATCATTTCTCATATTCAAA+TGG + chr4_2:50788059-50788078 None:intergenic 20.0%
!! TTAAAATTTGACATAAGGTT+TGG - chr4_2:50788482-50788501 Msa0605020:intron 20.0%
!!! CTAATTCAGTAACTAAATTT+TGG - chr4_2:50787921-50787940 Msa0605020:intron 20.0%
!! TATCATTTCTCATATTCAAA+TGG + chr4_2:50788059-50788078 None:intergenic 20.0%
!! TTAAAATTTGACATAAGGTT+TGG - chr4_2:50788482-50788501 Msa0605020:intron 20.0%
! TAATTAAGGTATGTGATTTG+TGG + chr4_2:50787483-50787502 None:intergenic 25.0%
!!! TTTTTTTGAAGTAAGTAGCT+AGG - chr4_2:50787631-50787650 Msa0605020:intron 25.0%
!!! TTCAACATTTTTTACTCAAG+AGG - chr4_2:50787660-50787679 Msa0605020:intron 25.0%
! AAAAGATTCAAAAGTTCCTT+TGG - chr4_2:50787786-50787805 Msa0605020:CDS 25.0%
! TTTAGTTACTGAATTAGAGA+CGG + chr4_2:50787918-50787937 None:intergenic 25.0%
! TTCTTATTGGTCAAAAACAA+TGG + chr4_2:50788160-50788179 None:intergenic 25.0%
!!! TTGTTTTTCTCACTTCTTAT+TGG + chr4_2:50788173-50788192 None:intergenic 25.0%
! AATAAGAAGTGAGAAAAACA+AGG - chr4_2:50788172-50788191 Msa0605020:intron 25.0%
! GCAATAACAAAATAATTAGC+TGG + chr4_2:50788266-50788285 None:intergenic 25.0%
! GAATCAAGCTTGAAAAAATT+AGG - chr4_2:50788339-50788358 Msa0605020:CDS 25.0%
! CTACTACACCATATTTAAAA+TGG + chr4_2:50788371-50788390 None:intergenic 25.0%
! TAATTAAGGTATGTGATTTG+TGG + chr4_2:50787483-50787502 None:intergenic 25.0%
!!! TTTTTTTGAAGTAAGTAGCT+AGG - chr4_2:50787631-50787650 Msa0605020:intron 25.0%
!!! TTCAACATTTTTTACTCAAG+AGG - chr4_2:50787660-50787679 Msa0605020:intron 25.0%
! AAAAGATTCAAAAGTTCCTT+TGG - chr4_2:50787786-50787805 Msa0605020:CDS 25.0%
! TTTAGTTACTGAATTAGAGA+CGG + chr4_2:50787918-50787937 None:intergenic 25.0%
! TTCTTATTGGTCAAAAACAA+TGG + chr4_2:50788160-50788179 None:intergenic 25.0%
!!! TTGTTTTTCTCACTTCTTAT+TGG + chr4_2:50788173-50788192 None:intergenic 25.0%
! AATAAGAAGTGAGAAAAACA+AGG - chr4_2:50788172-50788191 Msa0605020:intron 25.0%
! GCAATAACAAAATAATTAGC+TGG + chr4_2:50788266-50788285 None:intergenic 25.0%
! GAATCAAGCTTGAAAAAATT+AGG - chr4_2:50788339-50788358 Msa0605020:CDS 25.0%
! CTACTACACCATATTTAAAA+TGG + chr4_2:50788371-50788390 None:intergenic 25.0%
TAAGTGTTACGTGCTAATTA+AGG + chr4_2:50787497-50787516 None:intergenic 30.0%
AAGAGAAAGTTGTCAAAAAG+TGG + chr4_2:50788091-50788110 None:intergenic 30.0%
AAAACAATGGAGAGAGAAAA+AGG + chr4_2:50788147-50788166 None:intergenic 30.0%
TCAACAAAGTTGTCATCAAA+TGG - chr4_2:50788198-50788217 Msa0605020:intron 30.0%
! GGTGCTAACCATTTTAAATA+TGG - chr4_2:50788360-50788379 Msa0605020:CDS 30.0%
TAAGTGTTACGTGCTAATTA+AGG + chr4_2:50787497-50787516 None:intergenic 30.0%
AAGAGAAAGTTGTCAAAAAG+TGG + chr4_2:50788091-50788110 None:intergenic 30.0%
AAAACAATGGAGAGAGAAAA+AGG + chr4_2:50788147-50788166 None:intergenic 30.0%
TCAACAAAGTTGTCATCAAA+TGG - chr4_2:50788198-50788217 Msa0605020:intron 30.0%
! GGTGCTAACCATTTTAAATA+TGG - chr4_2:50788360-50788379 Msa0605020:CDS 30.0%
AACACTTACCCTTCAGAATA+TGG - chr4_2:50787509-50787528 Msa0605020:CDS 35.0%
ACACTTACCCTTCAGAATAT+GGG - chr4_2:50787510-50787529 Msa0605020:CDS 35.0%
AAGAAGAATTCTGCAGAGTT+AGG - chr4_2:50787600-50787619 Msa0605020:intron 35.0%
AAATCCGTCTCTAATGTACT+CGG + chr4_2:50787948-50787967 None:intergenic 35.0%
TGACAAAGTCAAAGCTTGAA+TGG + chr4_2:50788022-50788041 None:intergenic 35.0%
TCTCATATTCAAATGGTGAG+TGG + chr4_2:50788052-50788071 None:intergenic 35.0%
!! TTCAGGTACTGCTTCTTTTA+TGG + chr4_2:50788591-50788610 None:intergenic 35.0%
AACACTTACCCTTCAGAATA+TGG - chr4_2:50787509-50787528 Msa0605020:CDS 35.0%
ACACTTACCCTTCAGAATAT+GGG - chr4_2:50787510-50787529 Msa0605020:CDS 35.0%
AAGAAGAATTCTGCAGAGTT+AGG - chr4_2:50787600-50787619 Msa0605020:intron 35.0%
AAATCCGTCTCTAATGTACT+CGG + chr4_2:50787948-50787967 None:intergenic 35.0%
TGACAAAGTCAAAGCTTGAA+TGG + chr4_2:50788022-50788041 None:intergenic 35.0%
TCTCATATTCAAATGGTGAG+TGG + chr4_2:50788052-50788071 None:intergenic 35.0%
!! TTCAGGTACTGCTTCTTTTA+TGG + chr4_2:50788591-50788610 None:intergenic 35.0%
! CAAAGTGCCCATATTCTGAA+GGG + chr4_2:50787520-50787539 None:intergenic 40.0%
ATGGGCACTTTGGATACAAA+AGG - chr4_2:50787528-50787547 Msa0605020:CDS 40.0%
TGAAGAACAAGAAGCTCTTG+TGG - chr4_2:50787557-50787576 Msa0605020:CDS 40.0%
!! GAGAACAGTTTTTGAGCTGA+TGG + chr4_2:50787760-50787779 None:intergenic 40.0%
TACCATGAGAAAGACAGACA+TGG + chr4_2:50787839-50787858 None:intergenic 40.0%
AGAGAGAAAAAGGAAGAGAG+AGG + chr4_2:50788137-50788156 None:intergenic 40.0%
GTGAATCAGCAGTTCAATTG+CGG - chr4_2:50788292-50788311 Msa0605020:intron 40.0%
! TGTAGTAGACGAGCATTTTG+AGG - chr4_2:50788383-50788402 Msa0605020:CDS 40.0%
GGTGACAAAGTTTGCACTAT+TGG - chr4_2:50788562-50788581 Msa0605020:CDS 40.0%
AAGAAGCAGTACCTGAAATG+TGG - chr4_2:50788594-50788613 Msa0605020:CDS 40.0%
! CAAAGTGCCCATATTCTGAA+GGG + chr4_2:50787520-50787539 None:intergenic 40.0%
ATGGGCACTTTGGATACAAA+AGG - chr4_2:50787528-50787547 Msa0605020:CDS 40.0%
TGAAGAACAAGAAGCTCTTG+TGG - chr4_2:50787557-50787576 Msa0605020:CDS 40.0%
!! GAGAACAGTTTTTGAGCTGA+TGG + chr4_2:50787760-50787779 None:intergenic 40.0%
TACCATGAGAAAGACAGACA+TGG + chr4_2:50787839-50787858 None:intergenic 40.0%
AGAGAGAAAAAGGAAGAGAG+AGG + chr4_2:50788137-50788156 None:intergenic 40.0%
GTGAATCAGCAGTTCAATTG+CGG - chr4_2:50788292-50788311 Msa0605020:intron 40.0%
! TGTAGTAGACGAGCATTTTG+AGG - chr4_2:50788383-50788402 Msa0605020:CDS 40.0%
GGTGACAAAGTTTGCACTAT+TGG - chr4_2:50788562-50788581 Msa0605020:CDS 40.0%
AAGAAGCAGTACCTGAAATG+TGG - chr4_2:50788594-50788613 Msa0605020:CDS 40.0%
! CCAAAGTGCCCATATTCTGA+AGG + chr4_2:50787521-50787540 None:intergenic 45.0%
CCTTCAGAATATGGGCACTT+TGG - chr4_2:50787518-50787537 Msa0605020:CDS 45.0%
AAGCTCTTGTGGTGAAGTCA+TGG - chr4_2:50787568-50787587 Msa0605020:CDS 45.0%
!!! AGCTTGGTGTTTTGCTCCAA+AGG + chr4_2:50787805-50787824 None:intergenic 45.0%
AGAAAGACAGACATGGCATG+AGG + chr4_2:50787832-50787851 None:intergenic 45.0%
TGCCATGTCTGTCTTTCTCA+TGG - chr4_2:50787834-50787853 Msa0605020:CDS 45.0%
TGGACCGAGTACATTAGAGA+CGG - chr4_2:50787941-50787960 Msa0605020:intron 45.0%
AGTCAAAGCTTGAATGGTCG+TGG + chr4_2:50788016-50788035 None:intergenic 45.0%
GCAGTTCAATTGCGGAAATC+CGG - chr4_2:50788300-50788319 Msa0605020:intron 45.0%
TTCTCTGACCGTGACTTTAC+CGG + chr4_2:50788322-50788341 None:intergenic 45.0%
AGTACCTGAAATGTGGTCAC+CGG - chr4_2:50788601-50788620 Msa0605020:CDS 45.0%
ACCGGCTATGAAGAATGCAT+GGG - chr4_2:50788619-50788638 Msa0605020:CDS 45.0%
! CCAAAGTGCCCATATTCTGA+AGG + chr4_2:50787521-50787540 None:intergenic 45.0%
CCTTCAGAATATGGGCACTT+TGG - chr4_2:50787518-50787537 Msa0605020:CDS 45.0%
AAGCTCTTGTGGTGAAGTCA+TGG - chr4_2:50787568-50787587 Msa0605020:CDS 45.0%
!!! AGCTTGGTGTTTTGCTCCAA+AGG + chr4_2:50787805-50787824 None:intergenic 45.0%
AGAAAGACAGACATGGCATG+AGG + chr4_2:50787832-50787851 None:intergenic 45.0%
TGCCATGTCTGTCTTTCTCA+TGG - chr4_2:50787834-50787853 Msa0605020:CDS 45.0%
TGGACCGAGTACATTAGAGA+CGG - chr4_2:50787941-50787960 Msa0605020:intron 45.0%
AGTCAAAGCTTGAATGGTCG+TGG + chr4_2:50788016-50788035 None:intergenic 45.0%
GCAGTTCAATTGCGGAAATC+CGG - chr4_2:50788300-50788319 Msa0605020:intron 45.0%
TTCTCTGACCGTGACTTTAC+CGG + chr4_2:50788322-50788341 None:intergenic 45.0%
AGTACCTGAAATGTGGTCAC+CGG - chr4_2:50788601-50788620 Msa0605020:CDS 45.0%
ACCGGCTATGAAGAATGCAT+GGG - chr4_2:50788619-50788638 Msa0605020:CDS 45.0%
GCGGAAATCCGGTAAAGTCA+CGG - chr4_2:50788311-50788330 Msa0605020:intron 50.0%
ATAGCCGGTGACCACATTTC+AGG + chr4_2:50788608-50788627 None:intergenic 50.0%
! CACCGGCTATGAAGAATGCA+TGG - chr4_2:50788618-50788637 Msa0605020:CDS 50.0%
CCCCATGCATTCTTCATAGC+CGG + chr4_2:50788623-50788642 None:intergenic 50.0%
CCGGCTATGAAGAATGCATG+GGG - chr4_2:50788620-50788639 Msa0605020:CDS 50.0%
GGGAGAAGCTTATGATCAGC+TGG - chr4_2:50788640-50788659 Msa0605020:exon 50.0%
GCGGAAATCCGGTAAAGTCA+CGG - chr4_2:50788311-50788330 Msa0605020:intron 50.0%
ATAGCCGGTGACCACATTTC+AGG + chr4_2:50788608-50788627 None:intergenic 50.0%
! CACCGGCTATGAAGAATGCA+TGG - chr4_2:50788618-50788637 Msa0605020:CDS 50.0%
CCCCATGCATTCTTCATAGC+CGG + chr4_2:50788623-50788642 None:intergenic 50.0%
CCGGCTATGAAGAATGCATG+GGG - chr4_2:50788620-50788639 Msa0605020:CDS 50.0%
GGGAGAAGCTTATGATCAGC+TGG - chr4_2:50788640-50788659 Msa0605020:exon 50.0%
CATGGCATGAGGCTTGAGCT+TGG + chr4_2:50787821-50787840 None:intergenic 55.0%
CATGGCATGAGGCTTGAGCT+TGG + chr4_2:50787821-50787840 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 50787473 50788700 50787473 ID=Msa0605020;Name=Msa0605020
chr4_2 mRNA 50787473 50788700 50787473 ID=Msa0605020-mRNA-1;Parent=Msa0605020;Name=Msa0605020-mRNA-1;_AED=0.09;_eAED=0.09;_QI=55|1|0.75|1|1|1|4|0|160
chr4_2 exon 50787473 50787610 50787473 ID=Msa0605020-mRNA-1:exon:12031;Parent=Msa0605020-mRNA-1
chr4_2 exon 50787769 50787885 50787769 ID=Msa0605020-mRNA-1:exon:12030;Parent=Msa0605020-mRNA-1
chr4_2 exon 50788318 50788432 50788318 ID=Msa0605020-mRNA-1:exon:12029;Parent=Msa0605020-mRNA-1
chr4_2 exon 50788533 50788700 50788533 ID=Msa0605020-mRNA-1:exon:12028;Parent=Msa0605020-mRNA-1
chr4_2 five_prime_UTR 50788646 50788700 50788646 ID=Msa0605020-mRNA-1:five_prime_utr;Parent=Msa0605020-mRNA-1
chr4_2 CDS 50788533 50788645 50788533 ID=Msa0605020-mRNA-1:cds;Parent=Msa0605020-mRNA-1
chr4_2 CDS 50788318 50788432 50788318 ID=Msa0605020-mRNA-1:cds;Parent=Msa0605020-mRNA-1
chr4_2 CDS 50787769 50787885 50787769 ID=Msa0605020-mRNA-1:cds;Parent=Msa0605020-mRNA-1
chr4_2 CDS 50787473 50787610 50787473 ID=Msa0605020-mRNA-1:cds;Parent=Msa0605020-mRNA-1
Gene Sequence

>Msa0605020

ATGGGCACTTTGGATACAAAAGGTTTCACTGAAGAACAAGAAGCTCTTGTGGTGAAGTCATGGAATGCAATGAAGAAGAATTCTGCAGAGTTAGGACTAAAGCTTTTTTTGAAAATATTTGAGATTGCTCCATCAGCTCAAAAACTGTTCTCATTCTTAAAAGATTCAAAAGTTCCTTTGGAGCAAAACACCAAGCTCAAGCCTCATGCCATGTCTGTCTTTCTCATGACATGTGAATCAGCAGTTCAATTGCGGAAATCCGGTAAAGTCACGGTCAGAGAATCAAGCTTGAAAAAATTAGGTGCTAACCATTTTAAATATGGTGTAGTAGACGAGCATTTTGAGGTGACAAAGTTTGCACTATTGGAGACCATAAAAGAAGCAGTACCTGAAATGTGGTCACCGGCTATGAAGAATGCATGGGGAGAAGCTTATGATCAGCTGGTCAATGCTATCAAATCTGAAATGAAACCTTCCTCTTAA

Protein sequence

>Msa0605020

MGTLDTKGFTEEQEALVVKSWNAMKKNSAELGLKLFLKIFEIAPSAQKLFSFLKDSKVPLEQNTKLKPHAMSVFLMTCESAVQLRKSGKVTVRESSLKKLGANHFKYGVVDEHFEVTKFALLETIKEAVPEMWSPAMKNAWGEAYDQLVNAIKSEMKPSS*