AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0281010

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0281010 MtrunA17_Chr2g0295091 96.815 157 5 0 1 157 1 157 2.52e-107 302
Msa0281010 MtrunA17_Chr2g0295141 95.541 157 7 0 1 157 1 157 6.33e-107 301
Msa0281010 MtrunA17_Chr2g0295104 91.720 157 13 0 1 157 1 157 9.48e-102 288
Msa0281010 MtrunA17_Chr2g0295124 89.809 157 16 0 1 157 1 157 1.07e-98 281
Msa0281010 MtrunA17_Chr6g0462311 66.242 157 53 0 1 157 1 157 2.21e-70 209
Msa0281010 MtrunA17_Chr4g0067951 66.242 157 53 0 1 157 1 157 7.13e-70 208
Msa0281010 MtrunA17_Chr2g0295021 63.694 157 57 0 1 157 1 157 7.49e-68 202
Msa0281010 MtrunA17_Chr2g0295001 63.694 157 57 0 1 157 1 157 7.49e-68 202
Msa0281010 MtrunA17_Chr2g0295064 63.694 157 57 0 1 157 68 224 3.36e-66 201
Msa0281010 MtrunA17_Chr2g0295051 61.783 157 60 0 1 157 1 157 1.29e-65 197
Msa0281010 MtrunA17_Chr2g0295041 59.236 157 64 0 1 157 1 157 2.48e-62 189
Msa0281010 MtrunA17_Chr6g0462291 64.286 140 50 0 1 140 1 140 2.19e-59 181
Msa0281010 MtrunA17_Chr2g0295081 49.045 157 80 0 1 157 1 157 7.52e-50 157
Msa0281010 MtrunA17_Chr4g0067921 42.857 161 85 5 1 157 1 158 2.84e-32 112
Msa0281010 MtrunA17_Chr2g0295211 32.051 156 105 1 1 155 1 156 6.49e-30 106
Msa0281010 MtrunA17_Chr4g0067841 33.758 157 103 1 1 156 1 157 1.08e-29 106
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 65 sgRNAs with CRISPR-Local

Find 180 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCTATTGAGGACTTTAATT+TGG 0.098681 2_3:+16185857 Msa0281010:three_prime_UTR
GGTTTAATTGGCTAGTAATT+AGG 0.164037 2_3:-16185836 None:intergenic
GTGAGGTTTGAGTGCTATTT+TGG 0.186747 2_3:+16185990 Msa0281010:three_prime_UTR
AGTCCTCAATAGGGTTTAAT+TGG 0.205101 2_3:-16185848 None:intergenic
GTTTAATTGGCTAGTAATTA+GGG 0.278519 2_3:-16185835 None:intergenic
GGATAGCTTCTTAATAGTTC+CGG 0.311830 2_3:-16184950 None:intergenic
TACAGCTTAGTGGGAGGAAC+AGG 0.324066 2_3:+16185620 Msa0281010:CDS
CCTATTGAGGACTTTAATTT+GGG 0.325571 2_3:+16185858 Msa0281010:three_prime_UTR
CTTGATAAGTCCAGTTCCTT+TGG 0.329858 2_3:-16185786 None:intergenic
TACATGTGTGTTGGCTTTGT+TGG 0.344945 2_3:+16185962 Msa0281010:three_prime_UTR
TTCAACAATTTCAACACTTT+GGG 0.350751 2_3:-16184914 None:intergenic
CCCAAATTAAAGTCCTCAAT+AGG 0.362125 2_3:-16185858 None:intergenic
CTTCAACAATTTCAACACTT+TGG 0.364915 2_3:-16184915 None:intergenic
TGAGTATACATCAAGGTCTT+TGG 0.372404 2_3:+16185935 Msa0281010:three_prime_UTR
TGGGCAGCAGAAATCACCTT+TGG 0.387864 2_3:-16184895 None:intergenic
AGATAGAGTTGCATCACCTT+TGG 0.394288 2_3:-16185738 None:intergenic
GATAGCTTCTTAATAGTTCC+GGG 0.397748 2_3:-16184949 None:intergenic
GTTGAAATTGTTGAAGGAAA+TGG 0.402462 2_3:+16184922 Msa0281010:CDS
CCTATGTTGTGTTGGTGATA+AGG 0.406752 2_3:+16186030 Msa0281010:three_prime_UTR
CCAAATTAAAGTCCTCAATA+GGG 0.411845 2_3:-16185857 None:intergenic
GAGACTAAGGCCAAAGGAAC+TGG 0.415366 2_3:+16185776 Msa0281010:CDS
TTATCTTGATATATATATCA+TGG 0.416963 2_3:+16184783 Msa0281010:exon
AGAGCCTTGTAGAGTTTAGC+TGG 0.426244 2_3:-16184847 None:intergenic
GGGCAGCAGAAATCACCTTT+GGG 0.449440 2_3:-16184894 None:intergenic
TCAGTGGATGAGGCAAACTT+TGG 0.464386 2_3:+16185590 Msa0281010:CDS
TGCTAAAACGTAGCCCTCAA+TGG 0.474394 2_3:-16185810 None:intergenic
TGGATATAACTACAGCTTAG+TGG 0.476164 2_3:+16185610 Msa0281010:CDS
AAAATTGTTGCTGGCTCTGA+TGG 0.476544 2_3:+16185680 Msa0281010:CDS
TTTGGCTCGTACATGTGTGT+TGG 0.491488 2_3:+16185953 Msa0281010:three_prime_UTR
ATTAAATTAAATTAAAATGA+CGG 0.493877 2_3:-16186108 None:intergenic
CAAAGTGTTGAAATTGTTGA+AGG 0.502542 2_3:+16184916 Msa0281010:CDS
CAAATTAGATTCAGTGGATG+AGG 0.505903 2_3:+16185580 Msa0281010:CDS
GGATATAACTACAGCTTAGT+GGG 0.506709 2_3:+16185611 Msa0281010:CDS
ATCTGAATTACCTTCAACAA+TGG 0.509250 2_3:-16184971 None:intergenic
TAATTTGTGTAGCACAAAGT+TGG 0.512097 2_3:-16185564 None:intergenic
CCTTATCACCAACACAACAT+AGG 0.514089 2_3:-16186030 None:intergenic
GGACTTATCAAGGCCATTGA+GGG 0.514208 2_3:+16185797 Msa0281010:CDS
CAAAGGAACTGGACTTATCA+AGG 0.516806 2_3:+16185787 Msa0281010:CDS
AAGAAGCTATCCATTGTTGA+AGG 0.523757 2_3:+16184961 Msa0281010:CDS
AGCAGTACGTGAGGAGACTA+AGG 0.536209 2_3:+16185763 Msa0281010:CDS
GAGTTGCATCACCTTTGGTA+TGG 0.537356 2_3:-16185733 None:intergenic
TAGCCAATTAAACCCTATTG+AGG 0.542857 2_3:+16185845 Msa0281010:exon
AATAATTAAAGTTTATGATG+CGG 0.546841 2_3:+16185901 Msa0281010:three_prime_UTR
GGCTCCAGCTAAACTCTACA+AGG 0.556705 2_3:+16184843 Msa0281010:CDS
TATCTTGATATATATATCAT+GGG 0.556982 2_3:+16184784 Msa0281010:exon
GCTACACAAATTAGATTCAG+TGG 0.559555 2_3:+16185574 Msa0281010:CDS
TTTCACTGAAATCTTAACAA+TGG 0.567421 2_3:-16185708 None:intergenic
ATTGTTGCTGGCTCTGATGG+TGG 0.570543 2_3:+16185683 Msa0281010:CDS
TGGCTTTGTTGGATGTTGTG+AGG 0.571354 2_3:+16185973 Msa0281010:three_prime_UTR
TATATATTGCATAATACAGA+TGG 0.571471 2_3:-16186060 None:intergenic
TGGACTTATCAAGGCCATTG+AGG 0.572485 2_3:+16185796 Msa0281010:CDS
GTTGAAGGAAATGGAGGACC+CGG 0.599694 2_3:+16184931 Msa0281010:CDS
GTAGAGTTTAGCTGGAGCCA+CGG 0.602676 2_3:-16184839 None:intergenic
TGCTGATGAAATCGTCCCAA+AGG 0.606260 2_3:+16184879 Msa0281010:CDS
CTTAGTGGGAGGAACAGGGT+TGG 0.609437 2_3:+16185625 Msa0281010:CDS
ATATGCAAGAAGTAAATGAA+AGG 0.619747 2_3:-16184751 None:intergenic
GAAATTGTTGAAGGAAATGG+AGG 0.626795 2_3:+16184925 Msa0281010:CDS
TCAGTGAAATACCATACCAA+AGG 0.652605 2_3:+16185722 Msa0281010:CDS
CGTGAGGAGACTAAGGCCAA+AGG 0.670663 2_3:+16185770 Msa0281010:CDS
ATATATATATCAAGATAACA+AGG 0.672343 2_3:-16184778 None:intergenic
TCTATCTGAAGCAGTACGTG+AGG 0.676642 2_3:+16185754 Msa0281010:CDS
AGTGTGTTGAGTATACATCA+AGG 0.679518 2_3:+16185928 Msa0281010:three_prime_UTR
ACAGCTTAGTGGGAGGAACA+GGG 0.680453 2_3:+16185621 Msa0281010:CDS
TATAACTACAGCTTAGTGGG+AGG 0.697586 2_3:+16185614 Msa0281010:CDS
TGATGAACATGTCTCAACCG+TGG 0.717160 2_3:+16184822 Msa0281010:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTATATAAATAATTATAA+TGG + chr2_3:16185521-16185540 Msa0281010:intron 0.0%
!! AATTATATAAATAATTATAA+TGG + chr2_3:16185521-16185540 Msa0281010:intron 0.0%
!!! TTTGTCAATTTTTAAATAAA+AGG - chr2_3:16185116-16185135 None:intergenic 10.0%
!!! AAATAAAAGTATAACATAAA+TGG - chr2_3:16185327-16185346 None:intergenic 10.0%
!!! TTTGTCAATTTTTAAATAAA+AGG - chr2_3:16185116-16185135 None:intergenic 10.0%
!!! AAATAAAAGTATAACATAAA+TGG - chr2_3:16185327-16185346 None:intergenic 10.0%
!! ATATATATATCAAGATAACA+AGG - chr2_3:16184781-16184800 None:intergenic 15.0%
!! TTATCTTGATATATATATCA+TGG + chr2_3:16184783-16184802 Msa0281010:exon 15.0%
!! TATCTTGATATATATATCAT+GGG + chr2_3:16184784-16184803 Msa0281010:exon 15.0%
!!! AATTTTATACCCAAAAAAAA+AGG + chr2_3:16185240-16185259 Msa0281010:intron 15.0%
!!! ATTTAACTTCCTTTTTTTTT+GGG - chr2_3:16185252-16185271 None:intergenic 15.0%
!!! AATTTAACTTCCTTTTTTTT+TGG - chr2_3:16185253-16185272 None:intergenic 15.0%
!! AATAATTAAAGTTTATGATG+CGG + chr2_3:16185901-16185920 Msa0281010:three_prime_UTR 15.0%
!! ATATATATATCAAGATAACA+AGG - chr2_3:16184781-16184800 None:intergenic 15.0%
!! TTATCTTGATATATATATCA+TGG + chr2_3:16184783-16184802 Msa0281010:exon 15.0%
!! TATCTTGATATATATATCAT+GGG + chr2_3:16184784-16184803 Msa0281010:exon 15.0%
!!! AATTTTATACCCAAAAAAAA+AGG + chr2_3:16185240-16185259 Msa0281010:intron 15.0%
!!! ATTTAACTTCCTTTTTTTTT+GGG - chr2_3:16185252-16185271 None:intergenic 15.0%
!!! AATTTAACTTCCTTTTTTTT+TGG - chr2_3:16185253-16185272 None:intergenic 15.0%
!! AATAATTAAAGTTTATGATG+CGG + chr2_3:16185901-16185920 Msa0281010:three_prime_UTR 15.0%
!! AGTTAAATTCATTATACCAA+AGG + chr2_3:16185202-16185221 Msa0281010:intron 20.0%
!!! TAATTTAACTTTCTTTCCTT+TGG - chr2_3:16185221-16185240 None:intergenic 20.0%
!! AATAGTAATCCAATTCTATT+TGG + chr2_3:16185289-16185308 Msa0281010:intron 20.0%
!! TATATATTGCATAATACAGA+TGG - chr2_3:16186063-16186082 None:intergenic 20.0%
!! AGTTAAATTCATTATACCAA+AGG + chr2_3:16185202-16185221 Msa0281010:intron 20.0%
!!! TAATTTAACTTTCTTTCCTT+TGG - chr2_3:16185221-16185240 None:intergenic 20.0%
!! AATAGTAATCCAATTCTATT+TGG + chr2_3:16185289-16185308 Msa0281010:intron 20.0%
!! TATATATTGCATAATACAGA+TGG - chr2_3:16186063-16186082 None:intergenic 20.0%
! ATATGCAAGAAGTAAATGAA+AGG - chr2_3:16184754-16184773 None:intergenic 25.0%
! TTCAACAATTTCAACACTTT+GGG - chr2_3:16184917-16184936 None:intergenic 25.0%
! AATAGAATCTTGAGAAATGA+AGG + chr2_3:16185350-16185369 Msa0281010:intron 25.0%
! ATAATTATTAGACATAACCC+TGG - chr2_3:16185396-16185415 None:intergenic 25.0%
! AGGGTTATGTCTAATAATTA+TGG + chr2_3:16185395-16185414 Msa0281010:intron 25.0%
!!! CATCTGAATCATTTTTTTGA+AGG - chr2_3:16185422-16185441 None:intergenic 25.0%
! TTCAGATGATTAATGATACT+CGG + chr2_3:16185434-16185453 Msa0281010:intron 25.0%
! TATAATGGTTATATTGCAGA+TGG + chr2_3:16185536-16185555 Msa0281010:intron 25.0%
! TTTCACTGAAATCTTAACAA+TGG - chr2_3:16185711-16185730 None:intergenic 25.0%
! GTTTAATTGGCTAGTAATTA+GGG - chr2_3:16185838-16185857 None:intergenic 25.0%
! ATATGCAAGAAGTAAATGAA+AGG - chr2_3:16184754-16184773 None:intergenic 25.0%
! TTCAACAATTTCAACACTTT+GGG - chr2_3:16184917-16184936 None:intergenic 25.0%
! AATAGAATCTTGAGAAATGA+AGG + chr2_3:16185350-16185369 Msa0281010:intron 25.0%
! ATAATTATTAGACATAACCC+TGG - chr2_3:16185396-16185415 None:intergenic 25.0%
! AGGGTTATGTCTAATAATTA+TGG + chr2_3:16185395-16185414 Msa0281010:intron 25.0%
!!! CATCTGAATCATTTTTTTGA+AGG - chr2_3:16185422-16185441 None:intergenic 25.0%
! TTCAGATGATTAATGATACT+CGG + chr2_3:16185434-16185453 Msa0281010:intron 25.0%
! TATAATGGTTATATTGCAGA+TGG + chr2_3:16185536-16185555 Msa0281010:intron 25.0%
! TTTCACTGAAATCTTAACAA+TGG - chr2_3:16185711-16185730 None:intergenic 25.0%
! GTTTAATTGGCTAGTAATTA+GGG - chr2_3:16185838-16185857 None:intergenic 25.0%
CTTCAACAATTTCAACACTT+TGG - chr2_3:16184918-16184937 None:intergenic 30.0%
!! CAAAGTGTTGAAATTGTTGA+AGG + chr2_3:16184916-16184935 Msa0281010:CDS 30.0%
GTTGAAATTGTTGAAGGAAA+TGG + chr2_3:16184922-16184941 Msa0281010:CDS 30.0%
ATCTGAATTACCTTCAACAA+TGG - chr2_3:16184974-16184993 None:intergenic 30.0%
CATGACTATCCAAATAGAAT+TGG - chr2_3:16185301-16185320 None:intergenic 30.0%
! TAATTTGTGTAGCACAAAGT+TGG - chr2_3:16185567-16185586 None:intergenic 30.0%
! TTTGAGACAAAAATTGTTGC+TGG + chr2_3:16185671-16185690 Msa0281010:CDS 30.0%
GGTTTAATTGGCTAGTAATT+AGG - chr2_3:16185839-16185858 None:intergenic 30.0%
CCAAATTAAAGTCCTCAATA+GGG - chr2_3:16185860-16185879 None:intergenic 30.0%
! CCTATTGAGGACTTTAATTT+GGG + chr2_3:16185858-16185877 Msa0281010:three_prime_UTR 30.0%
AACAAAAACCTATGTTGTGT+TGG + chr2_3:16186022-16186041 Msa0281010:three_prime_UTR 30.0%
CTTCAACAATTTCAACACTT+TGG - chr2_3:16184918-16184937 None:intergenic 30.0%
!! CAAAGTGTTGAAATTGTTGA+AGG + chr2_3:16184916-16184935 Msa0281010:CDS 30.0%
GTTGAAATTGTTGAAGGAAA+TGG + chr2_3:16184922-16184941 Msa0281010:CDS 30.0%
ATCTGAATTACCTTCAACAA+TGG - chr2_3:16184974-16184993 None:intergenic 30.0%
CATGACTATCCAAATAGAAT+TGG - chr2_3:16185301-16185320 None:intergenic 30.0%
! TAATTTGTGTAGCACAAAGT+TGG - chr2_3:16185567-16185586 None:intergenic 30.0%
! TTTGAGACAAAAATTGTTGC+TGG + chr2_3:16185671-16185690 Msa0281010:CDS 30.0%
GGTTTAATTGGCTAGTAATT+AGG - chr2_3:16185839-16185858 None:intergenic 30.0%
CCAAATTAAAGTCCTCAATA+GGG - chr2_3:16185860-16185879 None:intergenic 30.0%
! CCTATTGAGGACTTTAATTT+GGG + chr2_3:16185858-16185877 Msa0281010:three_prime_UTR 30.0%
AACAAAAACCTATGTTGTGT+TGG + chr2_3:16186022-16186041 Msa0281010:three_prime_UTR 30.0%
GAAATTGTTGAAGGAAATGG+AGG + chr2_3:16184925-16184944 Msa0281010:CDS 35.0%
GATAGCTTCTTAATAGTTCC+GGG - chr2_3:16184952-16184971 None:intergenic 35.0%
GGATAGCTTCTTAATAGTTC+CGG - chr2_3:16184953-16184972 None:intergenic 35.0%
AAGAAGCTATCCATTGTTGA+AGG + chr2_3:16184961-16184980 Msa0281010:CDS 35.0%
! TTGTACCGACAAACTTTGAT+CGG - chr2_3:16185075-16185094 None:intergenic 35.0%
ATAATACATAAGCTCGTTCC+AGG + chr2_3:16185375-16185394 Msa0281010:intron 35.0%
TAATACATAAGCTCGTTCCA+GGG + chr2_3:16185376-16185395 Msa0281010:intron 35.0%
GCTACACAAATTAGATTCAG+TGG + chr2_3:16185574-16185593 Msa0281010:CDS 35.0%
CAAATTAGATTCAGTGGATG+AGG + chr2_3:16185580-16185599 Msa0281010:CDS 35.0%
TGGATATAACTACAGCTTAG+TGG + chr2_3:16185610-16185629 Msa0281010:CDS 35.0%
GGATATAACTACAGCTTAGT+GGG + chr2_3:16185611-16185630 Msa0281010:CDS 35.0%
TCAGTGAAATACCATACCAA+AGG + chr2_3:16185722-16185741 Msa0281010:CDS 35.0%
TAGCCAATTAAACCCTATTG+AGG + chr2_3:16185845-16185864 Msa0281010:exon 35.0%
AGTCCTCAATAGGGTTTAAT+TGG - chr2_3:16185851-16185870 None:intergenic 35.0%
CCCTATTGAGGACTTTAATT+TGG + chr2_3:16185857-16185876 Msa0281010:three_prime_UTR 35.0%
CCCAAATTAAAGTCCTCAAT+AGG - chr2_3:16185861-16185880 None:intergenic 35.0%
AGTGTGTTGAGTATACATCA+AGG + chr2_3:16185928-16185947 Msa0281010:three_prime_UTR 35.0%
TGAGTATACATCAAGGTCTT+TGG + chr2_3:16185935-16185954 Msa0281010:three_prime_UTR 35.0%
!!! TGAGGTTTGAGTGCTATTTT+GGG + chr2_3:16185991-16186010 Msa0281010:three_prime_UTR 35.0%
GAAATTGTTGAAGGAAATGG+AGG + chr2_3:16184925-16184944 Msa0281010:CDS 35.0%
GATAGCTTCTTAATAGTTCC+GGG - chr2_3:16184952-16184971 None:intergenic 35.0%
GGATAGCTTCTTAATAGTTC+CGG - chr2_3:16184953-16184972 None:intergenic 35.0%
AAGAAGCTATCCATTGTTGA+AGG + chr2_3:16184961-16184980 Msa0281010:CDS 35.0%
! TTGTACCGACAAACTTTGAT+CGG - chr2_3:16185075-16185094 None:intergenic 35.0%
ATAATACATAAGCTCGTTCC+AGG + chr2_3:16185375-16185394 Msa0281010:intron 35.0%
TAATACATAAGCTCGTTCCA+GGG + chr2_3:16185376-16185395 Msa0281010:intron 35.0%
GCTACACAAATTAGATTCAG+TGG + chr2_3:16185574-16185593 Msa0281010:CDS 35.0%
CAAATTAGATTCAGTGGATG+AGG + chr2_3:16185580-16185599 Msa0281010:CDS 35.0%
TGGATATAACTACAGCTTAG+TGG + chr2_3:16185610-16185629 Msa0281010:CDS 35.0%
GGATATAACTACAGCTTAGT+GGG + chr2_3:16185611-16185630 Msa0281010:CDS 35.0%
TCAGTGAAATACCATACCAA+AGG + chr2_3:16185722-16185741 Msa0281010:CDS 35.0%
TAGCCAATTAAACCCTATTG+AGG + chr2_3:16185845-16185864 Msa0281010:exon 35.0%
AGTCCTCAATAGGGTTTAAT+TGG - chr2_3:16185851-16185870 None:intergenic 35.0%
CCCTATTGAGGACTTTAATT+TGG + chr2_3:16185857-16185876 Msa0281010:three_prime_UTR 35.0%
CCCAAATTAAAGTCCTCAAT+AGG - chr2_3:16185861-16185880 None:intergenic 35.0%
AGTGTGTTGAGTATACATCA+AGG + chr2_3:16185928-16185947 Msa0281010:three_prime_UTR 35.0%
TGAGTATACATCAAGGTCTT+TGG + chr2_3:16185935-16185954 Msa0281010:three_prime_UTR 35.0%
!!! TGAGGTTTGAGTGCTATTTT+GGG + chr2_3:16185991-16186010 Msa0281010:three_prime_UTR 35.0%
GACAAACTTTGATCGGTTGT+CGG - chr2_3:16185068-16185087 None:intergenic 40.0%
GACAACCGATCAAAGTTTGT+CGG + chr2_3:16185067-16185086 Msa0281010:intron 40.0%
GACAAACATGATGCGACAAA+TGG + chr2_3:16185130-16185149 Msa0281010:intron 40.0%
TATAACTACAGCTTAGTGGG+AGG + chr2_3:16185614-16185633 Msa0281010:CDS 40.0%
!! AAAATTGTTGCTGGCTCTGA+TGG + chr2_3:16185680-16185699 Msa0281010:CDS 40.0%
AGATAGAGTTGCATCACCTT+TGG - chr2_3:16185741-16185760 None:intergenic 40.0%
CTTGATAAGTCCAGTTCCTT+TGG - chr2_3:16185789-16185808 None:intergenic 40.0%
CAAAGGAACTGGACTTATCA+AGG + chr2_3:16185787-16185806 Msa0281010:CDS 40.0%
!! TACATGTGTGTTGGCTTTGT+TGG + chr2_3:16185962-16185981 Msa0281010:three_prime_UTR 40.0%
!!! GTGAGGTTTGAGTGCTATTT+TGG + chr2_3:16185990-16186009 Msa0281010:three_prime_UTR 40.0%
CCTTATCACCAACACAACAT+AGG - chr2_3:16186033-16186052 None:intergenic 40.0%
!! CCTATGTTGTGTTGGTGATA+AGG + chr2_3:16186030-16186049 Msa0281010:three_prime_UTR 40.0%
GACAAACTTTGATCGGTTGT+CGG - chr2_3:16185068-16185087 None:intergenic 40.0%
GACAACCGATCAAAGTTTGT+CGG + chr2_3:16185067-16185086 Msa0281010:intron 40.0%
GACAAACATGATGCGACAAA+TGG + chr2_3:16185130-16185149 Msa0281010:intron 40.0%
TATAACTACAGCTTAGTGGG+AGG + chr2_3:16185614-16185633 Msa0281010:CDS 40.0%
!! AAAATTGTTGCTGGCTCTGA+TGG + chr2_3:16185680-16185699 Msa0281010:CDS 40.0%
AGATAGAGTTGCATCACCTT+TGG - chr2_3:16185741-16185760 None:intergenic 40.0%
CTTGATAAGTCCAGTTCCTT+TGG - chr2_3:16185789-16185808 None:intergenic 40.0%
CAAAGGAACTGGACTTATCA+AGG + chr2_3:16185787-16185806 Msa0281010:CDS 40.0%
!! TACATGTGTGTTGGCTTTGT+TGG + chr2_3:16185962-16185981 Msa0281010:three_prime_UTR 40.0%
!!! GTGAGGTTTGAGTGCTATTT+TGG + chr2_3:16185990-16186009 Msa0281010:three_prime_UTR 40.0%
CCTTATCACCAACACAACAT+AGG - chr2_3:16186033-16186052 None:intergenic 40.0%
!! CCTATGTTGTGTTGGTGATA+AGG + chr2_3:16186030-16186049 Msa0281010:three_prime_UTR 40.0%
TGATGAACATGTCTCAACCG+TGG + chr2_3:16184822-16184841 Msa0281010:CDS 45.0%
! AGAGCCTTGTAGAGTTTAGC+TGG - chr2_3:16184850-16184869 None:intergenic 45.0%
TGCTGATGAAATCGTCCCAA+AGG + chr2_3:16184879-16184898 Msa0281010:CDS 45.0%
TCAGTGGATGAGGCAAACTT+TGG + chr2_3:16185590-16185609 Msa0281010:CDS 45.0%
GAGTTGCATCACCTTTGGTA+TGG - chr2_3:16185736-16185755 None:intergenic 45.0%
! TCTATCTGAAGCAGTACGTG+AGG + chr2_3:16185754-16185773 Msa0281010:CDS 45.0%
TGGACTTATCAAGGCCATTG+AGG + chr2_3:16185796-16185815 Msa0281010:CDS 45.0%
GGACTTATCAAGGCCATTGA+GGG + chr2_3:16185797-16185816 Msa0281010:CDS 45.0%
TGCTAAAACGTAGCCCTCAA+TGG - chr2_3:16185813-16185832 None:intergenic 45.0%
! TTTGGCTCGTACATGTGTGT+TGG + chr2_3:16185953-16185972 Msa0281010:three_prime_UTR 45.0%
!! TGGCTTTGTTGGATGTTGTG+AGG + chr2_3:16185973-16185992 Msa0281010:three_prime_UTR 45.0%
TGATGAACATGTCTCAACCG+TGG + chr2_3:16184822-16184841 Msa0281010:CDS 45.0%
! AGAGCCTTGTAGAGTTTAGC+TGG - chr2_3:16184850-16184869 None:intergenic 45.0%
TGCTGATGAAATCGTCCCAA+AGG + chr2_3:16184879-16184898 Msa0281010:CDS 45.0%
TCAGTGGATGAGGCAAACTT+TGG + chr2_3:16185590-16185609 Msa0281010:CDS 45.0%
GAGTTGCATCACCTTTGGTA+TGG - chr2_3:16185736-16185755 None:intergenic 45.0%
! TCTATCTGAAGCAGTACGTG+AGG + chr2_3:16185754-16185773 Msa0281010:CDS 45.0%
TGGACTTATCAAGGCCATTG+AGG + chr2_3:16185796-16185815 Msa0281010:CDS 45.0%
GGACTTATCAAGGCCATTGA+GGG + chr2_3:16185797-16185816 Msa0281010:CDS 45.0%
TGCTAAAACGTAGCCCTCAA+TGG - chr2_3:16185813-16185832 None:intergenic 45.0%
! TTTGGCTCGTACATGTGTGT+TGG + chr2_3:16185953-16185972 Msa0281010:three_prime_UTR 45.0%
!! TGGCTTTGTTGGATGTTGTG+AGG + chr2_3:16185973-16185992 Msa0281010:three_prime_UTR 45.0%
!! ATTAAATTAAATTAAAATGA+CGG - chr2_3:16186111-16186130 None:intergenic 5.0%
!! ATTAAATTAAATTAAAATGA+CGG - chr2_3:16186111-16186130 None:intergenic 5.0%
GTAGAGTTTAGCTGGAGCCA+CGG - chr2_3:16184842-16184861 None:intergenic 50.0%
GGCTCCAGCTAAACTCTACA+AGG + chr2_3:16184843-16184862 Msa0281010:CDS 50.0%
GGGCAGCAGAAATCACCTTT+GGG - chr2_3:16184897-16184916 None:intergenic 50.0%
TGGGCAGCAGAAATCACCTT+TGG - chr2_3:16184898-16184917 None:intergenic 50.0%
GTTGAAGGAAATGGAGGACC+CGG + chr2_3:16184931-16184950 Msa0281010:CDS 50.0%
TACAGCTTAGTGGGAGGAAC+AGG + chr2_3:16185620-16185639 Msa0281010:CDS 50.0%
ACAGCTTAGTGGGAGGAACA+GGG + chr2_3:16185621-16185640 Msa0281010:CDS 50.0%
!! ATTGTTGCTGGCTCTGATGG+TGG + chr2_3:16185683-16185702 Msa0281010:CDS 50.0%
AGCAGTACGTGAGGAGACTA+AGG + chr2_3:16185763-16185782 Msa0281010:CDS 50.0%
GAGACTAAGGCCAAAGGAAC+TGG + chr2_3:16185776-16185795 Msa0281010:CDS 50.0%
GTAGAGTTTAGCTGGAGCCA+CGG - chr2_3:16184842-16184861 None:intergenic 50.0%
GGCTCCAGCTAAACTCTACA+AGG + chr2_3:16184843-16184862 Msa0281010:CDS 50.0%
GGGCAGCAGAAATCACCTTT+GGG - chr2_3:16184897-16184916 None:intergenic 50.0%
TGGGCAGCAGAAATCACCTT+TGG - chr2_3:16184898-16184917 None:intergenic 50.0%
GTTGAAGGAAATGGAGGACC+CGG + chr2_3:16184931-16184950 Msa0281010:CDS 50.0%
TACAGCTTAGTGGGAGGAAC+AGG + chr2_3:16185620-16185639 Msa0281010:CDS 50.0%
ACAGCTTAGTGGGAGGAACA+GGG + chr2_3:16185621-16185640 Msa0281010:CDS 50.0%
!! ATTGTTGCTGGCTCTGATGG+TGG + chr2_3:16185683-16185702 Msa0281010:CDS 50.0%
AGCAGTACGTGAGGAGACTA+AGG + chr2_3:16185763-16185782 Msa0281010:CDS 50.0%
GAGACTAAGGCCAAAGGAAC+TGG + chr2_3:16185776-16185795 Msa0281010:CDS 50.0%
CTTAGTGGGAGGAACAGGGT+TGG + chr2_3:16185625-16185644 Msa0281010:CDS 55.0%
CGTGAGGAGACTAAGGCCAA+AGG + chr2_3:16185770-16185789 Msa0281010:CDS 55.0%
CTTAGTGGGAGGAACAGGGT+TGG + chr2_3:16185625-16185644 Msa0281010:CDS 55.0%
CGTGAGGAGACTAAGGCCAA+AGG + chr2_3:16185770-16185789 Msa0281010:CDS 55.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 16184705 16186146 16184705 ID=Msa0281010;Name=Msa0281010
chr2_3 mRNA 16184705 16186146 16184705 ID=Msa0281010-mRNA-1;Parent=Msa0281010;Name=Msa0281010-mRNA-1;_AED=0.26;_eAED=0.26;_QI=97|1|1|1|1|1|2|299|157
chr2_3 exon 16184705 16184982 16184705 ID=Msa0281010-mRNA-1:exon:6666;Parent=Msa0281010-mRNA-1
chr2_3 exon 16185555 16186146 16185555 ID=Msa0281010-mRNA-1:exon:6667;Parent=Msa0281010-mRNA-1
chr2_3 five_prime_UTR 16184705 16184801 16184705 ID=Msa0281010-mRNA-1:five_prime_utr;Parent=Msa0281010-mRNA-1
chr2_3 CDS 16184802 16184982 16184802 ID=Msa0281010-mRNA-1:cds;Parent=Msa0281010-mRNA-1
chr2_3 CDS 16185555 16185847 16185555 ID=Msa0281010-mRNA-1:cds;Parent=Msa0281010-mRNA-1
chr2_3 three_prime_UTR 16185848 16186146 16185848 ID=Msa0281010-mRNA-1:three_prime_utr;Parent=Msa0281010-mRNA-1
Gene Sequence

>Msa0281010

ATGGGTGTTTTTACTTTCAATGATGAACATGTCTCAACCGTGGCTCCAGCTAAACTCTACAAGGCTCTTGCAAAAGATGCTGATGAAATCGTCCCAAAGGTGATTTCTGCTGCCCAAAGTGTTGAAATTGTTGAAGGAAATGGAGGACCCGGAACTATTAAGAAGCTATCCATTGTTGAAGATGGCAAAACCAACTTTGTGCTACACAAATTAGATTCAGTGGATGAGGCAAACTTTGGATATAACTACAGCTTAGTGGGAGGAACAGGGTTGGATGAAAGTTTAGAGAAAGTTGAATTTGAGACAAAAATTGTTGCTGGCTCTGATGGTGGATCCATTGTTAAGATTTCAGTGAAATACCATACCAAAGGTGATGCAACTCTATCTGAAGCAGTACGTGAGGAGACTAAGGCCAAAGGAACTGGACTTATCAAGGCCATTGAGGGCTACGTTTTAGCAAACCCTAATTACTAG
Protein sequence

>Msa0281010

MGVFTFNDEHVSTVAPAKLYKALAKDADEIVPKVISAAQSVEIVEGNGGPGTIKKLSIVEDGKTNFVLHKLDSVDEANFGYNYSLVGGTGLDESLEKVEFETKIVAGSDGGSIVKISVKYHTKGDATLSEAVREETKAKGTGLIKAIEGYVLANPNY*