AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0330500

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0330500 MtrunA17_Chr1g0210231 82.353 51 6 2 38 87 221 269 4.27e-19 80.1
Msa0330500 MtrunA17_Chr8g0360481 68.627 51 13 2 38 87 203 251 1.87e-14 67.0
Msa0330500 MtrunA17_Chr1g0188261 50.725 69 28 2 19 87 170 232 9.23e-12 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0330500 AT3G10420.2 74.510 51 10 2 38 87 232 280 5.07e-16 71.6
Msa0330500 AT3G10420.1 74.510 51 10 2 38 87 232 280 7.44e-16 71.2
Msa0330500 AT1G73170.1 62.745 51 16 2 38 87 216 264 1.47e-12 62.0
Msa0330500 AT1G73170.3 62.745 51 16 2 38 87 216 264 1.89e-12 61.6
Msa0330500 AT1G73170.2 62.745 51 16 2 38 87 88 136 2.40e-12 61.2
Msa0330500 AT1G33290.1 50.000 70 27 3 19 87 169 231 7.21e-12 59.7
Msa0330500 AT1G33290.3 50.000 70 27 3 19 87 95 157 8.99e-12 59.3

Find 53 sgRNAs with CRISPR-Local

Find 185 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGGTCCCTCATTTGGCTTT+GGG 0.251206 2_4:+44978931 Msa0330500:three_prime_UTR
TCTGGTCCCTCATTTGGCTT+TGG 0.257586 2_4:+44978930 Msa0330500:three_prime_UTR
GGTTCCTCTGGTCCCTCATT+TGG 0.282892 2_4:+44978924 Msa0330500:three_prime_UTR
TTCAATGGGTTTAGTAATAT+CGG 0.290969 2_4:+44975727 Msa0330500:CDS
ACAAAGATGATGCAAGAAAT+TGG 0.300447 2_4:+44978968 Msa0330500:three_prime_UTR
GTGGATACATCCAATGAAAT+TGG 0.303806 2_4:+44977043 Msa0330500:CDS
GGTGGAGATGTTCTTCTTGC+AGG 0.311714 2_4:+44977067 Msa0330500:CDS
TTCATTGCTGCTGGTTCCTC+TGG 0.329963 2_4:+44978912 Msa0330500:three_prime_UTR
TCATCTTTGTCCTATTCTAT+AGG 0.336405 2_4:-44978955 Msa0330510:intron
ATCTCCACCTCCAATTTCAT+TGG 0.369281 2_4:-44977053 None:intergenic
CAATGCTGCATATTCACATT+TGG 0.374960 2_4:-44977124 None:intergenic
TGTTGGCTCTTCATTGCTGC+TGG 0.375727 2_4:+44978903 Msa0330500:three_prime_UTR
GTTGTTGTTAGATCTTTCAA+TGG 0.390304 2_4:+44975712 Msa0330500:CDS
GGGCTTAAATACTACTTGTT+GGG 0.410633 2_4:+44979154 Msa0330500:three_prime_UTR
TGGGCTTAAATACTACTTGT+TGG 0.414964 2_4:+44979153 Msa0330500:three_prime_UTR
GTGTGAAGGATTCAGCAATA+TGG 0.428666 2_4:+44979113 Msa0330500:three_prime_UTR
AAAGAGGATGCAAGTATTGT+TGG 0.439321 2_4:+44978886 Msa0330500:three_prime_UTR
CATGAAGCACGTAGCGATAG+TGG 0.449986 2_4:+44977024 Msa0330500:CDS
TTGTTGTTAGATCTTTCAAT+GGG 0.450923 2_4:+44975713 Msa0330500:CDS
CAAATGTGAATATGCAGCAT+TGG 0.470780 2_4:+44977125 Msa0330500:CDS
GGCTTTGGGACCTATAGAAT+AGG 0.480062 2_4:+44978945 Msa0330500:three_prime_UTR
GTTGCTGTTAGATCTTTCAA+TGG 0.483907 2_4:+44975687 Msa0330500:intron
GATGTTCTTCTTGCAGGCAT+AGG 0.487244 2_4:+44977073 Msa0330500:CDS
ATGTCTGAAGAAGCTTTGAT+TGG 0.490771 2_4:+44976969 Msa0330500:intron
ATACTTGCATCCTCTTTACA+TGG 0.501795 2_4:-44978880 Msa0330510:intron
TAAAGTTGAACATGGTAGAA+AGG 0.506168 2_4:-44979327 Msa0330510:intron
ATAAACTTTGAACATACAAA+AGG 0.508397 2_4:+44979003 Msa0330500:three_prime_UTR
TTGATTGGGCAGAGACTGCT+AGG 0.514063 2_4:+44976984 Msa0330500:CDS
TTGCTGTTAGATCTTTCAAT+GGG 0.517313 2_4:+44975688 Msa0330500:intron
TGGCAAATGAATGGCAATGT+TGG 0.517863 2_4:+44979133 Msa0330500:three_prime_UTR
GGCAAATGAATGGCAATGTT+GGG 0.524844 2_4:+44979134 Msa0330500:three_prime_UTR
CCACTGTCAGCATGAGAGAA+AGG 0.525406 2_4:-44979263 Msa0330510:intron
TAAATACATCATATTCTGCT+TGG 0.529650 2_4:-44979077 Msa0330510:intron
AAATGTGAATATGCAGCATT+GGG 0.532173 2_4:+44977126 Msa0330500:CDS
AACATACAAAAGGAGAAGAA+AGG 0.533617 2_4:+44979013 Msa0330500:three_prime_UTR
GAATCCTTCACACTATCTGC+AGG 0.534749 2_4:-44979103 Msa0330510:intron
CTTAAATACTACTTGTTGGG+AGG 0.541392 2_4:+44979157 Msa0330500:three_prime_UTR
CTTCTTGCAGGCATAGGCAT+AGG 0.545790 2_4:+44977079 Msa0330500:CDS
ACATCCAATGAAATTGGAGG+TGG 0.549569 2_4:+44977049 Msa0330500:CDS
GGCAGAGACTGCTAGGATGT+TGG 0.549676 2_4:+44976991 Msa0330500:CDS
ATAGGTCCCAAAGCCAAATG+AGG 0.563237 2_4:-44978937 Msa0330510:intron
TCAGCAATATGGCAAATGAA+TGG 0.566898 2_4:+44979124 Msa0330500:three_prime_UTR
CCTTTCTCTCATGCTGACAG+TGG 0.568781 2_4:+44979263 Msa0330500:three_prime_UTR
AGGATTAGAACCATGTAAAG+AGG 0.571409 2_4:+44978870 Msa0330500:intron
TGTCTGAAGAAGCTTTGATT+GGG 0.584292 2_4:+44976970 Msa0330500:intron
AGCACCTGCAGATAGTGTGA+AGG 0.594876 2_4:+44979099 Msa0330500:three_prime_UTR
GATACATCCAATGAAATTGG+AGG 0.601573 2_4:+44977046 Msa0330500:CDS
GCATAGGCATAGGCAAGGCA+AGG 0.608211 2_4:+44977089 Msa0330500:CDS
TGCAGGCATAGGCATAGGCA+AGG 0.611887 2_4:+44977084 Msa0330500:CDS
TAGGTCCCAAAGCCAAATGA+GGG 0.633837 2_4:-44978936 Msa0330510:intron
TTTACATGTAAAGTTGAACA+TGG 0.647592 2_4:-44979335 Msa0330510:intron
AAAGCCAAATGAGGGACCAG+AGG 0.666546 2_4:-44978928 Msa0330510:intron
TAGGCATAGGCAAGGCAAGG+AGG 0.705253 2_4:+44977092 Msa0330500:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAAAAAAAAAAAAAAATT+AGG - chr2_4:44977339-44977358 None:intergenic 0.0%
!!! ATTTTCTTTCAAAAAAAAAA+AGG + chr2_4:44976078-44976097 Msa0330500:intron 10.0%
!! TTAGATTAATTAACAAAAAA+AGG + chr2_4:44978068-44978087 Msa0330500:intron 10.0%
!! ATCTAATAACATAAAAAAAG+GGG - chr2_4:44975628-44975647 None:intergenic 15.0%
!! GATCTAATAACATAAAAAAA+GGG - chr2_4:44975629-44975648 None:intergenic 15.0%
!! AGATCTAATAACATAAAAAA+AGG - chr2_4:44975630-44975649 None:intergenic 15.0%
!! TTTAATTAGAGTTGAATAAT+AGG + chr2_4:44976121-44976140 Msa0330500:intron 15.0%
!!! TAATTATATGTTGAGAATTT+TGG + chr2_4:44976160-44976179 Msa0330500:intron 15.0%
!! TTACTTACATAAAATAAGTT+TGG - chr2_4:44976311-44976330 None:intergenic 15.0%
!! GTAAAAGAAAATAACAATAA+CGG + chr2_4:44976626-44976645 Msa0330500:intron 15.0%
!!! AAATTTTAATGTATGTGTTA+AGG - chr2_4:44976722-44976741 None:intergenic 15.0%
!! TCAAACTAGATAAAATAAAA+AGG - chr2_4:44976949-44976968 None:intergenic 15.0%
!! AAATTCTCAAAAAGATAAAA+AGG - chr2_4:44978001-44978020 None:intergenic 15.0%
!! AAGTTTAAATAATACTGAAA+TGG + chr2_4:44978360-44978379 Msa0330500:intron 15.0%
!! AGTTTAAATAATACTGAAAT+GGG + chr2_4:44978361-44978380 Msa0330500:intron 15.0%
!! TTTATTCTTATGTTAATGAT+AGG + chr2_4:44978850-44978869 Msa0330500:intron 15.0%
!!! CTTTACATTTTTACATAGTT+TGG - chr2_4:44975593-44975612 None:intergenic 20.0%
!! TCTAATAACATAAAAAAAGG+GGG - chr2_4:44975627-44975646 None:intergenic 20.0%
!! TTAATGATATGTAGAAAAGT+AGG + chr2_4:44975979-44975998 Msa0330500:intron 20.0%
!! ACAAAATAAATAAGAACAGT+AGG - chr2_4:44976035-44976054 None:intergenic 20.0%
!!! TTATTTTGTGCAAATGTATT+TGG + chr2_4:44976046-44976065 Msa0330500:intron 20.0%
!! TATATGTGTTAAAAACTAAC+CGG - chr2_4:44976453-44976472 None:intergenic 20.0%
!! AAAATAAAAAGGAAACTGAT+AGG - chr2_4:44976938-44976957 None:intergenic 20.0%
!!! ATTTTAGTAAGGTTTATGTA+AGG - chr2_4:44977412-44977431 None:intergenic 20.0%
!! TTGTTAAAAGGATATTCATT+TGG - chr2_4:44977621-44977640 None:intergenic 20.0%
!! TGTGTTATATGATTGTTAAA+AGG - chr2_4:44977633-44977652 None:intergenic 20.0%
!!! AATACCAATCTCTATTATTT+TGG + chr2_4:44977697-44977716 Msa0330500:intron 20.0%
!!! GCAATTAAATATTATCCTTT+TGG - chr2_4:44977845-44977864 None:intergenic 20.0%
!!! ATAAGTAGCATTTTTTTAGT+AGG + chr2_4:44977926-44977945 Msa0330500:intron 20.0%
!! AAGGATTCGATAAAAAAAAT+AGG - chr2_4:44977982-44978001 None:intergenic 20.0%
!! GTTTAAATAATACTGAAATG+GGG + chr2_4:44978362-44978381 Msa0330500:intron 20.0%
!!! ATGTTAATGAACCATTTTTT+TGG + chr2_4:44978813-44978832 Msa0330500:intron 20.0%
!! TTTATGTTTGTCCAAAAAAA+TGG - chr2_4:44978827-44978846 Msa0330510:intron 20.0%
!! ATAAACTTTGAACATACAAA+AGG + chr2_4:44979003-44979022 Msa0330500:three_prime_UTR 20.0%
!! TTGTTGTTAGATCTTTCAAT+GGG + chr2_4:44975713-44975732 Msa0330500:CDS 25.0%
!! TTCAATGGGTTTAGTAATAT+CGG + chr2_4:44975727-44975746 Msa0330500:CDS 25.0%
!! AAGTGGGATCTTATATTTTT+CGG + chr2_4:44975766-44975785 Msa0330500:intron 25.0%
!! ATATGTTGAGAATTTTGGAT+AGG + chr2_4:44976165-44976184 Msa0330500:intron 25.0%
!! GAATTTTGGATAGGAAAAAA+TGG + chr2_4:44976174-44976193 Msa0330500:intron 25.0%
! TCATGTTGAGATATAAATTG+AGG - chr2_4:44976265-44976284 None:intergenic 25.0%
! TAATTTCGATATATGCTTCA+AGG - chr2_4:44976343-44976362 None:intergenic 25.0%
! GACTTACGAAAAACTAAAAA+GGG - chr2_4:44976394-44976413 None:intergenic 25.0%
! ATTAGCATATCGAATAAGTT+TGG - chr2_4:44976416-44976435 None:intergenic 25.0%
! TATTCGATATGCTAATGAAT+TGG + chr2_4:44976420-44976439 Msa0330500:intron 25.0%
! AACTCATTTAAATGATTGCA+GGG + chr2_4:44976784-44976803 Msa0330500:intron 25.0%
! AGTCAAACATATCACAAAAA+GGG - chr2_4:44977202-44977221 None:intergenic 25.0%
! TAGTCAAACATATCACAAAA+AGG - chr2_4:44977203-44977222 None:intergenic 25.0%
! TTGATGTTGAGATCTAAATT+GGG - chr2_4:44977248-44977267 None:intergenic 25.0%
! TTTGATGTTGAGATCTAAAT+TGG - chr2_4:44977249-44977268 None:intergenic 25.0%
! GACTTACATAAAATGAAGTT+TGG - chr2_4:44977293-44977312 None:intergenic 25.0%
! AAAAATTAGGATATGCTTCA+AGG - chr2_4:44977326-44977345 None:intergenic 25.0%
! ATGAAGAAAAGTGATGTAAA+TGG - chr2_4:44977464-44977483 None:intergenic 25.0%
!! TCACTAAAAAATCAGTGTTT+TGG + chr2_4:44977563-44977582 Msa0330500:intron 25.0%
! GATACCAAAATAATAGAGAT+TGG - chr2_4:44977704-44977723 None:intergenic 25.0%
!! AAAAATGCTACTTATATGCT+TGG - chr2_4:44977921-44977940 None:intergenic 25.0%
!!! TTTTTAGTAGGATCATTTGA+TGG + chr2_4:44977938-44977957 Msa0330500:intron 25.0%
! TAAAAAAAATAGGCAAGTGA+GGG - chr2_4:44977972-44977991 None:intergenic 25.0%
! ATAAAAAAAATAGGCAAGTG+AGG - chr2_4:44977973-44977992 None:intergenic 25.0%
! GACATAACACAAGAAAAAAT+CGG - chr2_4:44978135-44978154 None:intergenic 25.0%
!!! TTTTTTCTTGTGTTATGTCT+AGG + chr2_4:44978136-44978155 Msa0330500:intron 25.0%
! TAATACAATTACACAAGCAA+AGG - chr2_4:44978205-44978224 None:intergenic 25.0%
! AATAACAACAATATTGAGTG+AGG + chr2_4:44978295-44978314 Msa0330500:intron 25.0%
! ATTTCTACCTACTTAACATT+TGG + chr2_4:44978427-44978446 Msa0330500:intron 25.0%
! TAAATACATCATATTCTGCT+TGG - chr2_4:44979080-44979099 Msa0330510:intron 25.0%
! TTTACATGTAAAGTTGAACA+TGG - chr2_4:44979338-44979357 Msa0330510:intron 25.0%
AAAAAAAGGGGGAAATAGTT+AGG - chr2_4:44975616-44975635 None:intergenic 30.0%
! TTGCTGTTAGATCTTTCAAT+GGG + chr2_4:44975688-44975707 Msa0330500:intron 30.0%
! GTTGTTGTTAGATCTTTCAA+TGG + chr2_4:44975712-44975731 Msa0330500:CDS 30.0%
TGATGATACTAAACTGAAGT+GGG + chr2_4:44975750-44975769 Msa0330500:intron 30.0%
TTGTGCAAATGTATTTGGTT+AGG + chr2_4:44976051-44976070 Msa0330500:intron 30.0%
GATATAAATTGACGAAGTCT+TGG - chr2_4:44976226-44976245 None:intergenic 30.0%
GGACTTACGAAAAACTAAAA+AGG - chr2_4:44976395-44976414 None:intergenic 30.0%
CAAATATCTGAGTATCTGAT+AGG + chr2_4:44976540-44976559 Msa0330500:intron 30.0%
ATGTTTCATGATTTAGTGGT+TGG + chr2_4:44976574-44976593 Msa0330500:intron 30.0%
GAACTCATTTAAATGATTGC+AGG + chr2_4:44976783-44976802 Msa0330500:intron 30.0%
ACTCATTTAAATGATTGCAG+GGG + chr2_4:44976785-44976804 Msa0330500:intron 30.0%
TTGATATGTCTATTGTGAAC+TGG + chr2_4:44976825-44976844 Msa0330500:intron 30.0%
! TGAACTGGTTTACTATTTAC+TGG + chr2_4:44976840-44976859 Msa0330500:intron 30.0%
AAATGTGAATATGCAGCATT+GGG + chr2_4:44977126-44977145 Msa0330500:CDS 30.0%
TGATGTTGAGATCTAAATTG+GGG - chr2_4:44977247-44977266 None:intergenic 30.0%
! TTTTCAATCACCATGAATGA+AGG + chr2_4:44977360-44977379 Msa0330500:intron 30.0%
TAGTAAGGTTTATGTAAGGA+AGG - chr2_4:44977408-44977427 None:intergenic 30.0%
!!! GGGAAGGTATTATTTTAGTA+AGG - chr2_4:44977423-44977442 None:intergenic 30.0%
!! AAAAATCAGTGTTTTGGACT+TGG + chr2_4:44977569-44977588 Msa0330500:intron 30.0%
!! AAAATCAGTGTTTTGGACTT+GGG + chr2_4:44977570-44977589 Msa0330500:intron 30.0%
! TTTTCAGTGATGTAGATAGA+AGG - chr2_4:44977670-44977689 None:intergenic 30.0%
TCTATCTACATCACTGAAAA+TGG + chr2_4:44977670-44977689 Msa0330500:intron 30.0%
! AGATATTCAAGATTTTGAGC+TGG + chr2_4:44978624-44978643 Msa0330500:intron 30.0%
! GATATTCAAGATTTTGAGCT+GGG + chr2_4:44978625-44978644 Msa0330500:intron 30.0%
!! TTTTTCTCCTTTGAGTTATG+TGG - chr2_4:44978726-44978745 Msa0330510:CDS 30.0%
TGTAAATCTGTGTTTGAACA+GGG - chr2_4:44978751-44978770 Msa0330510:intron 30.0%
TCATCTTTGTCCTATTCTAT+AGG - chr2_4:44978958-44978977 Msa0330510:intron 30.0%
ACAAAGATGATGCAAGAAAT+TGG + chr2_4:44978968-44978987 Msa0330500:three_prime_UTR 30.0%
AACATACAAAAGGAGAAGAA+AGG + chr2_4:44979013-44979032 Msa0330500:three_prime_UTR 30.0%
AAAGGAAAAACAACTCATGA+AGG + chr2_4:44979031-44979050 Msa0330500:three_prime_UTR 30.0%
TAAAGTTGAACATGGTAGAA+AGG - chr2_4:44979330-44979349 Msa0330510:intron 30.0%
CTAACAGCAACAATGAAAAC+AGG - chr2_4:44975678-44975697 None:intergenic 35.0%
! GTTGCTGTTAGATCTTTCAA+TGG + chr2_4:44975687-44975706 Msa0330500:intron 35.0%
GTGATGATACTAAACTGAAG+TGG + chr2_4:44975749-44975768 Msa0330500:intron 35.0%
CTTCTCATATGTAATCACAG+AGG + chr2_4:44975795-44975814 Msa0330500:intron 35.0%
AAATTGAGGAAGACAAGTTG+AGG - chr2_4:44976251-44976270 None:intergenic 35.0%
CTAATGAATTGGAGTTATGC+CGG + chr2_4:44976431-44976450 Msa0330500:intron 35.0%
! ATACTCCAACAAGTTGAAAG+TGG - chr2_4:44976491-44976510 None:intergenic 35.0%
GTGGATGTTTCATGATTTAG+TGG + chr2_4:44976570-44976589 Msa0330500:intron 35.0%
! AGTTTCTTTGAAAATGCCTC+TGG + chr2_4:44976896-44976915 Msa0330500:intron 35.0%
AAAACAGAAACAAAGTCCAG+AGG - chr2_4:44976915-44976934 None:intergenic 35.0%
! ATGTCTGAAGAAGCTTTGAT+TGG + chr2_4:44976969-44976988 Msa0330500:intron 35.0%
! TGTCTGAAGAAGCTTTGATT+GGG + chr2_4:44976970-44976989 Msa0330500:intron 35.0%
GTGGATACATCCAATGAAAT+TGG + chr2_4:44977043-44977062 Msa0330500:CDS 35.0%
GATACATCCAATGAAATTGG+AGG + chr2_4:44977046-44977065 Msa0330500:CDS 35.0%
CAATGCTGCATATTCACATT+TGG - chr2_4:44977127-44977146 None:intergenic 35.0%
CAAATGTGAATATGCAGCAT+TGG + chr2_4:44977125-44977144 Msa0330500:CDS 35.0%
TGGTACTAATCTCACTCATT+TGG + chr2_4:44977150-44977169 Msa0330500:intron 35.0%
! GTGATGTAAATGGTGATGAA+GGG - chr2_4:44977454-44977473 None:intergenic 35.0%
! AGTGATGTAAATGGTGATGA+AGG - chr2_4:44977455-44977474 None:intergenic 35.0%
!! AAATCAGTGTTTTGGACTTG+GGG + chr2_4:44977571-44977590 Msa0330500:intron 35.0%
! TTTGGCATCTGTGATTTTGA+GGG - chr2_4:44977603-44977622 None:intergenic 35.0%
! ATTTGGCATCTGTGATTTTG+AGG - chr2_4:44977604-44977623 None:intergenic 35.0%
TTTAGGTCTCAACATCCAAA+AGG + chr2_4:44977827-44977846 Msa0330500:intron 35.0%
ACAACAATATTGAGTGAGGA+TGG + chr2_4:44978299-44978318 Msa0330500:intron 35.0%
CAACAATATTGAGTGAGGAT+GGG + chr2_4:44978300-44978319 Msa0330500:intron 35.0%
CTTCAAGCCAAATGTTAAGT+AGG - chr2_4:44978437-44978456 None:intergenic 35.0%
TCAAAGTTTCCCAATGTGTT+TGG - chr2_4:44978680-44978699 Msa0330510:CDS 35.0%
ACATTGGGAAACTTTGATGA+AGG + chr2_4:44978683-44978702 Msa0330500:intron 35.0%
TTTGCTTCCACATAACTCAA+AGG + chr2_4:44978716-44978735 Msa0330500:intron 35.0%
GTGTAAATCTGTGTTTGAAC+AGG - chr2_4:44978752-44978771 Msa0330510:intron 35.0%
AGGATTAGAACCATGTAAAG+AGG + chr2_4:44978870-44978889 Msa0330500:intron 35.0%
ATACTTGCATCCTCTTTACA+TGG - chr2_4:44978883-44978902 Msa0330510:intron 35.0%
! AAAGAGGATGCAAGTATTGT+TGG + chr2_4:44978886-44978905 Msa0330500:three_prime_UTR 35.0%
TCAGCAATATGGCAAATGAA+TGG + chr2_4:44979124-44979143 Msa0330500:three_prime_UTR 35.0%
TGGGCTTAAATACTACTTGT+TGG + chr2_4:44979153-44979172 Msa0330500:three_prime_UTR 35.0%
GGGCTTAAATACTACTTGTT+GGG + chr2_4:44979154-44979173 Msa0330500:three_prime_UTR 35.0%
! CTTAAATACTACTTGTTGGG+AGG + chr2_4:44979157-44979176 Msa0330500:three_prime_UTR 35.0%
AGCTACCACTTTCAACTTGT+TGG + chr2_4:44976483-44976502 Msa0330500:intron 40.0%
GTATCTGATAGGAAAATGCG+TGG + chr2_4:44976551-44976570 Msa0330500:intron 40.0%
ACATCCAATGAAATTGGAGG+TGG + chr2_4:44977049-44977068 Msa0330500:CDS 40.0%
ATCTCCACCTCCAATTTCAT+TGG - chr2_4:44977056-44977075 None:intergenic 40.0%
GTTGAGATCTAAATTGGGGA+AGG - chr2_4:44977243-44977262 None:intergenic 40.0%
ACAATGCACTCCTTCATTCA+TGG - chr2_4:44977373-44977392 None:intergenic 40.0%
ATGAAGGGTTTATACAGGGA+AGG - chr2_4:44977439-44977458 None:intergenic 40.0%
GGTGATGAAGGGTTTATACA+GGG - chr2_4:44977443-44977462 None:intergenic 40.0%
! TGGTGATGAAGGGTTTATAC+AGG - chr2_4:44977444-44977463 None:intergenic 40.0%
!! ACACCTAGAGTGCTCATTTT+AGG + chr2_4:44977810-44977829 Msa0330500:intron 40.0%
AGACCTAAAATGAGCACTCT+AGG - chr2_4:44977816-44977835 None:intergenic 40.0%
! TAGTAGGATCATTTGATGGC+TGG + chr2_4:44977942-44977961 Msa0330500:intron 40.0%
AAAAAATAGGCAAGTGAGGG+AGG - chr2_4:44977969-44977988 None:intergenic 40.0%
GGGAGATTAGAATGCATTGA+AGG + chr2_4:44978645-44978664 Msa0330500:intron 40.0%
GAATGCATTGAAGGAGTTGT+TGG + chr2_4:44978654-44978673 Msa0330500:intron 40.0%
AGTTGTTGGCCAAACACATT+GGG + chr2_4:44978668-44978687 Msa0330500:intron 40.0%
GTGTGAAGGATTCAGCAATA+TGG + chr2_4:44979113-44979132 Msa0330500:three_prime_UTR 40.0%
TGGCAAATGAATGGCAATGT+TGG + chr2_4:44979133-44979152 Msa0330500:three_prime_UTR 40.0%
GGCAAATGAATGGCAATGTT+GGG + chr2_4:44979134-44979153 Msa0330500:three_prime_UTR 40.0%
!!! TCCTTTTTTCATGCTGACAG+TGG - chr2_4:44979421-44979440 Msa0330510:intron 40.0%
GATGTTCTTCTTGCAGGCAT+AGG + chr2_4:44977073-44977092 Msa0330500:CDS 45.0%
! GTGAATATGCAGCATTGGGT+TGG + chr2_4:44977130-44977149 Msa0330500:intron 45.0%
! AGAGTGCTCAGTTTAGTGCT+CGG + chr2_4:44977880-44977899 Msa0330500:intron 45.0%
GAGTTGTTGGCCAAACACAT+TGG + chr2_4:44978667-44978686 Msa0330500:intron 45.0%
GGAAACTTTGATGAAGGAGC+TGG + chr2_4:44978689-44978708 Msa0330500:intron 45.0%
TAGGTCCCAAAGCCAAATGA+GGG - chr2_4:44978939-44978958 Msa0330510:intron 45.0%
ATAGGTCCCAAAGCCAAATG+AGG - chr2_4:44978940-44978959 Msa0330510:intron 45.0%
!! GGCTTTGGGACCTATAGAAT+AGG + chr2_4:44978945-44978964 Msa0330500:three_prime_UTR 45.0%
GAATCCTTCACACTATCTGC+AGG - chr2_4:44979106-44979125 Msa0330510:intron 45.0%
! ATGCTGACAGTGGCTTTTGT+GGG + chr2_4:44979273-44979292 Msa0330500:three_prime_UTR 45.0%
GCCACTGTCAGCATGAAAAA+AGG + chr2_4:44979417-44979436 Msa0330500:three_prime_UTR 45.0%
AGAATCCTGCTCTTCGCATC+AGG + chr2_4:44976512-44976531 Msa0330500:intron 50.0%
TTGATTGGGCAGAGACTGCT+AGG + chr2_4:44976984-44977003 Msa0330500:CDS 50.0%
CATGAAGCACGTAGCGATAG+TGG + chr2_4:44977024-44977043 Msa0330500:CDS 50.0%
GGTGGAGATGTTCTTCTTGC+AGG + chr2_4:44977067-44977086 Msa0330500:CDS 50.0%
CTTCTTGCAGGCATAGGCAT+AGG + chr2_4:44977079-44977098 Msa0330500:CDS 50.0%
!! TGTTGGCTCTTCATTGCTGC+TGG + chr2_4:44978903-44978922 Msa0330500:three_prime_UTR 50.0%
!! TTCATTGCTGCTGGTTCCTC+TGG + chr2_4:44978912-44978931 Msa0330500:three_prime_UTR 50.0%
AAAGCCAAATGAGGGACCAG+AGG - chr2_4:44978931-44978950 Msa0330510:intron 50.0%
! TCTGGTCCCTCATTTGGCTT+TGG + chr2_4:44978930-44978949 Msa0330500:three_prime_UTR 50.0%
!! CTGGTCCCTCATTTGGCTTT+GGG + chr2_4:44978931-44978950 Msa0330500:three_prime_UTR 50.0%
AGCACCTGCAGATAGTGTGA+AGG + chr2_4:44979099-44979118 Msa0330500:three_prime_UTR 50.0%
CCACTGTCAGCATGAGAGAA+AGG - chr2_4:44979266-44979285 Msa0330510:intron 50.0%
CCTTTCTCTCATGCTGACAG+TGG + chr2_4:44979263-44979282 Msa0330500:three_prime_UTR 50.0%
! CATGCTGACAGTGGCTTTTG+TGG + chr2_4:44979272-44979291 Msa0330500:three_prime_UTR 50.0%
! GCGGCTGTTACAGCTTTTGT+GGG - chr2_4:44979450-44979469 Msa0330510:intron 50.0%
CATCACCTGATGCGAAGAGC+AGG - chr2_4:44976520-44976539 None:intergenic 55.0%
GGCAGAGACTGCTAGGATGT+TGG + chr2_4:44976991-44977010 Msa0330500:CDS 55.0%
TGCAGGCATAGGCATAGGCA+AGG + chr2_4:44977084-44977103 Msa0330500:CDS 55.0%
GCATAGGCATAGGCAAGGCA+AGG + chr2_4:44977089-44977108 Msa0330500:CDS 55.0%
TAGGCATAGGCAAGGCAAGG+AGG + chr2_4:44977092-44977111 Msa0330500:CDS 55.0%
GGTTCCTCTGGTCCCTCATT+TGG + chr2_4:44978924-44978943 Msa0330500:three_prime_UTR 55.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 44975574 44979469 44975574 ID=Msa0330500;Name=Msa0330500
chr2_4 mRNA 44975574 44979469 44975574 ID=Msa0330500-mRNA-1;Parent=Msa0330500;Name=Msa0330500-mRNA-1;_AED=0.21;_eAED=0.38;_QI=0|0|0.25|1|0|0|4|592|89
chr2_4 exon 44975574 44975598 44975574 ID=Msa0330500-mRNA-1:exon:13714;Parent=Msa0330500-mRNA-1
chr2_4 exon 44975698 44975767 44975698 ID=Msa0330500-mRNA-1:exon:13715;Parent=Msa0330500-mRNA-1
chr2_4 exon 44976979 44977147 44976979 ID=Msa0330500-mRNA-1:exon:13716;Parent=Msa0330500-mRNA-1
chr2_4 exon 44978872 44979469 44978872 ID=Msa0330500-mRNA-1:exon:13717;Parent=Msa0330500-mRNA-1
chr2_4 CDS 44975574 44975598 44975574 ID=Msa0330500-mRNA-1:cds;Parent=Msa0330500-mRNA-1
chr2_4 CDS 44975698 44975767 44975698 ID=Msa0330500-mRNA-1:cds;Parent=Msa0330500-mRNA-1
chr2_4 CDS 44976979 44977147 44976979 ID=Msa0330500-mRNA-1:cds;Parent=Msa0330500-mRNA-1
chr2_4 CDS 44978872 44978877 44978872 ID=Msa0330500-mRNA-1:cds;Parent=Msa0330500-mRNA-1
chr2_4 three_prime_UTR 44978878 44979469 44978878 ID=Msa0330500-mRNA-1:three_prime_utr;Parent=Msa0330500-mRNA-1
Gene Sequence

>Msa0330500

ATGGAGGATCATCGCACCAAACTATATCTTTCAATGGGTGTTGTTGTTAGATCTTTCAATGGGTTTAGTAATATCGGTGATGATACTAAACTGAAAAGCTTTGATTGGGCAGAGACTGCTAGGATGTTGGCAGATGAGTTCATGAAGCACGTAGCGATAGTGGATACATCCAATGAAATTGGAGGTGGAGATGTTCTTCTTGCAGGCATAGGCATAGGCAAGGCAAGGAGGATGCAAGTTCCAAATGTGAATATGCAGCATTGGGATTAG
Protein sequence

>Msa0330500

MEDHRTKLYLSMGVVVRSFNGFSNIGDDTKLKSFDWAETARMLADEFMKHVAIVDTSNEIGGGDVLLAGIGIGKARRMQVPNVNMQHWD*