AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0384190

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0384190 MtrunA17_Chr3g0141681 96.774 310 10 0 1 310 1 310 0.0 603
Msa0384190 MtrunA17_Chr3g0141691 76.452 310 71 1 1 310 1 308 1.06e-173 483
Msa0384190 MtrunA17_Chr3g0144701 72.876 306 81 1 5 310 9 312 1.26e-167 468
Msa0384190 MtrunA17_Chr5g0404471 70.227 309 90 1 2 310 3 309 9.10e-158 442
Msa0384190 MtrunA17_Chr4g0035131 61.765 306 115 1 3 308 43 346 2.53e-138 395
Msa0384190 MtrunA17_Chr4g0035111 58.442 308 126 1 1 308 3 308 1.28e-132 380
Msa0384190 MtrunA17_Chr5g0404511 57.321 321 123 4 1 310 1 318 4.52e-124 357
Msa0384190 MtrunA17_Chr5g0404481 57.009 321 124 4 1 310 1 318 1.38e-123 356
Msa0384190 MtrunA17_Chr4g0035121 49.032 310 154 2 3 310 2 309 1.47e-103 305
Msa0384190 MtrunA17_Chr5g0409651 46.645 313 155 5 4 309 3 310 1.47e-93 280
Msa0384190 MtrunA17_Chr8g0369391 46.178 314 160 4 3 310 2 312 1.20e-92 277
Msa0384190 MtrunA17_Chr3g0078901 45.338 311 160 5 2 310 3 305 1.08e-90 273
Msa0384190 MtrunA17_Chr5g0409641 46.452 310 154 5 4 306 3 307 1.42e-90 272
Msa0384190 MtrunA17_Chr3g0078831 44.839 310 163 4 2 310 3 305 1.57e-87 265
Msa0384190 MtrunA17_Chr1g0198771 39.159 309 183 3 4 310 13 318 5.10e-75 234
Msa0384190 MtrunA17_Chr3g0078941 43.961 207 109 3 104 310 12 211 7.42e-54 175
Msa0384190 MtrunA17_Chr3g0078951 50.000 106 49 3 2 106 3 105 9.22e-23 91.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0384190 AT4G39230.1 72.258 310 84 1 1 310 1 308 1.87e-166 465
Msa0384190 AT1G75280.1 71.987 307 85 1 3 309 4 309 3.85e-157 441
Msa0384190 AT1G75290.1 67.532 308 100 0 2 309 3 310 2.14e-152 430
Msa0384190 AT1G75290.2 67.532 308 100 0 2 309 30 337 1.03e-151 429
Msa0384190 AT1G75300.1 63.043 322 102 3 2 309 3 321 2.90e-141 402
Msa0384190 AT1G19540.1 60.390 308 120 1 5 310 3 310 2.67e-129 371
Msa0384190 AT1G19540.2 62.451 253 93 1 5 255 3 255 4.83e-105 307
Msa0384190 AT4G34540.1 47.727 308 154 2 3 310 6 306 1.10e-99 295
Msa0384190 AT1G32100.1 44.586 314 164 4 3 310 8 317 1.19e-83 255
Msa0384190 AT4G13660.1 44.762 315 164 4 2 310 7 317 1.22e-83 255
Msa0384190 AT4G13660.2 44.762 315 164 4 2 310 7 317 1.22e-83 255

Find 93 sgRNAs with CRISPR-Local

Find 196 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCAATAGCACGTCGAATTT+TGG 0.137471 3_1:+87271650 None:intergenic
ACATTGAACAAATGGAATTT+AGG 0.142995 3_1:-87270538 Msa0384190:three_prime_UTR
TTGAATGAATAAATGTTCTT+TGG 0.240740 3_1:+87271220 None:intergenic
TTGTCAAAATGTTGCCTTCT+TGG 0.255177 3_1:-87270687 Msa0384190:three_prime_UTR
TGAATGAATAAATGTTCTTT+GGG 0.284153 3_1:+87271221 None:intergenic
CTCAATAATTTCAAAACTTT+AGG 0.291956 3_1:-87273066 Msa0384190:CDS
CATTGAACAAATGGAATTTA+GGG 0.292864 3_1:-87270537 Msa0384190:three_prime_UTR
GAAGATTATCGCTGCAATTA+AGG 0.319373 3_1:-87272737 Msa0384190:CDS
TATATGACGACTTTGTCTTT+TGG 0.336858 3_1:+87271531 None:intergenic
CAAAACTTTAGGCGTCAATT+TGG 0.338853 3_1:-87273055 Msa0384190:CDS
ATATGAGTTGTTTGTTACTT+TGG 0.343840 3_1:-87270821 Msa0384190:three_prime_UTR
AAAGACAAAGTCGTCATATA+TGG 0.346716 3_1:-87271528 Msa0384190:CDS
TTTGTGATTGAGCCATCTTT+TGG 0.365839 3_1:-87270950 Msa0384190:CDS
ACAGTTGTGTACTTGACATC+TGG 0.375136 3_1:+87270905 None:intergenic
GCCTTGTGGGAGAAGAAGAT+TGG 0.381870 3_1:-87271187 Msa0384190:CDS
TGGATCATCCACAGCTCTAA+TGG 0.384230 3_1:+87271276 None:intergenic
ATCGAAAGTAATTAAGGAAC+AGG 0.389535 3_1:-87273252 Msa0384190:five_prime_UTR
GTGTCTAGCAACTACTTTGC+TGG 0.392084 3_1:-87271603 Msa0384190:CDS
GAATTGATCGAAAGTAATTA+AGG 0.402098 3_1:-87273258 Msa0384190:five_prime_UTR
ATCGATAATACCACGTTGAT+TGG 0.408391 3_1:+87271010 None:intergenic
GGAGGTGGGGCGAATTGTCC+TGG 0.408477 3_1:+87271555 None:intergenic
AGAATCTTATTCAATGTCCT+TGG 0.431387 3_1:+87271256 None:intergenic
CAGGGGAAACATTGAACAAA+TGG 0.436351 3_1:-87270546 Msa0384190:three_prime_UTR
CAACGAGCTTGTAGCCTTGT+GGG 0.437114 3_1:-87271200 Msa0384190:CDS
AAACTTCTCAAGGATATTCA+AGG 0.440563 3_1:-87271130 Msa0384190:intron
TTTCATTTGCTATGTAAACT+TGG 0.451614 3_1:-87270753 Msa0384190:three_prime_UTR
TAAGGAGGCTGGTAACGTTA+AGG 0.454571 3_1:-87272719 Msa0384190:intron
TTGAGGAGATTAGCCTTGGC+AGG 0.461709 3_1:+87273084 None:intergenic
AATACCACGTTGATTGGAAT+AGG 0.469491 3_1:+87271016 None:intergenic
TCCTTGAGAAGTTTATCCTC+TGG 0.484267 3_1:+87271139 None:intergenic
CTCCCCACATTAGCTCAGCC+AGG 0.490923 3_1:-87271573 Msa0384190:CDS
GATCAGCGAGGGTGGATTCA+CGG 0.492277 3_1:+87273106 None:intergenic
TGCTTAGTCAAACTATGAGC+AGG 0.501678 3_1:-87270565 Msa0384190:three_prime_UTR
CCAGCAAAATCTGCATTTGA+AGG 0.504040 3_1:-87271678 Msa0384190:CDS
TTGCCTATAATTAAGAGGGC+TGG 0.507755 3_1:-87273326 None:intergenic
TTCACGGACCAATGCAAAAG+TGG 0.510674 3_1:+87273122 None:intergenic
TACTTCAAACGAACTGATCA+AGG 0.512935 3_1:+87270873 None:intergenic
ATCGCTGCAATTAAGGAGGC+TGG 0.516620 3_1:-87272730 Msa0384190:CDS
GTAGAAGCAAGTGCAAAAGC+TGG 0.517859 3_1:-87273150 Msa0384190:CDS
GGTAGGCCACGCTCTGATTG+AGG 0.518193 3_1:-87272767 Msa0384190:CDS
ATTATTGAGGAGATTAGCCT+TGG 0.522079 3_1:+87273080 None:intergenic
TCCAGAGGATAAACTTCTCA+AGG 0.522426 3_1:-87271140 Msa0384190:CDS
CCTTGTGGGAGAAGAAGATT+GGG 0.523149 3_1:-87271186 Msa0384190:CDS
TAACACACGCGTTGCATCCA+TGG 0.523365 3_1:-87273229 Msa0384190:exon
CACCACTCGGTGTTTGTGAA+AGG 0.525734 3_1:-87270986 Msa0384190:CDS
TTGTCCTGGCTGAGCTAATG+TGG 0.535528 3_1:+87271569 None:intergenic
TGTCTAGCAACTACTTTGCT+GGG 0.539689 3_1:-87271602 Msa0384190:CDS
TAAGCCTCAACCCCAAAAGA+TGG 0.540084 3_1:+87270938 None:intergenic
CCCAATCTTCTTCTCCCACA+AGG 0.547082 3_1:+87271186 None:intergenic
CAGTTGTGTACTTGACATCT+GGG 0.555264 3_1:+87270906 None:intergenic
GTTGCTAGACACATATGTGT+AGG 0.558044 3_1:+87271614 None:intergenic
TCAACGAGCTTGTAGCCTTG+TGG 0.567546 3_1:-87271201 Msa0384190:CDS
GAATGAATAAATGTTCTTTG+GGG 0.568027 3_1:+87271222 None:intergenic
AATGAATAAATGTTCTTTGG+GGG 0.568257 3_1:+87271223 None:intergenic
TCTGTATGATCATGAGAACT+TGG 0.570219 3_1:-87272827 Msa0384190:CDS
AACATTCACCATTAGAGCTG+TGG 0.581419 3_1:-87271284 Msa0384190:CDS
AGTAATCAAGCAGGTAGATG+TGG 0.585296 3_1:-87272800 Msa0384190:CDS
GGTAGATGTGGTCATATCTA+CGG 0.586142 3_1:-87272788 Msa0384190:CDS
TGTCCTGGCTGAGCTAATGT+GGG 0.586984 3_1:+87271570 None:intergenic
CTTGGTGAAAGTAATCAAGC+AGG 0.588181 3_1:-87272809 Msa0384190:CDS
TTGCTAGACACATATGTGTA+GGG 0.589350 3_1:+87271615 None:intergenic
GCTCATAGTTTGACTAAGCA+CGG 0.593610 3_1:+87270568 None:intergenic
CACCCTCGCTGATCCTGCCA+AGG 0.595340 3_1:-87273097 Msa0384190:CDS
GCTTAGTCAAACTATGAGCA+GGG 0.598406 3_1:-87270564 Msa0384190:three_prime_UTR
AAAGTCGTCATATATGGCGA+TGG 0.599327 3_1:-87271522 Msa0384190:CDS
GGTATTATCGATCCACCACT+CGG 0.602530 3_1:-87270999 Msa0384190:CDS
CAGCAAAATCTGCATTTGAA+GGG 0.606294 3_1:-87271677 Msa0384190:CDS
CGACGTGCTATTGAAGCGGA+AGG 0.614282 3_1:-87271642 Msa0384190:CDS
AAACTCAGATGACACCAAGA+AGG 0.618173 3_1:+87270673 None:intergenic
TTAGAGCTGTGGATGATCCA+AGG 0.628287 3_1:-87271273 Msa0384190:CDS
ATTGAACAAATGGAATTTAG+GGG 0.628382 3_1:-87270536 Msa0384190:three_prime_UTR
AATTCGACGTGCTATTGAAG+CGG 0.628628 3_1:-87271646 Msa0384190:CDS
CTTTCACAAACACCGAGTGG+TGG 0.628695 3_1:+87270987 None:intergenic
TCACGGACCAATGCAAAAGT+GGG 0.629925 3_1:+87273123 None:intergenic
TTCAACAAGGAAGATGACAT+TGG 0.630695 3_1:-87271307 Msa0384190:CDS
CTTGGCAGGATCAGCGAGGG+TGG 0.631457 3_1:+87273098 None:intergenic
ACGTCATTCCCAAATTCCGA+AGG 0.641793 3_1:+87271723 None:intergenic
ATCGGAGGAACAGGTTACAT+CGG 0.642196 3_1:-87273183 Msa0384190:CDS
GATGTGGTCATATCTACGGT+AGG 0.649142 3_1:-87272784 Msa0384190:CDS
CTTGATCCTCAATCAGAGCG+TGG 0.652635 3_1:+87272761 None:intergenic
ATGTACAGCGGTGTTCAACA+AGG 0.653602 3_1:-87271320 Msa0384190:intron
TGACGTTGATCGTGTCCATG+CGG 0.662937 3_1:-87271706 Msa0384190:CDS
AGCCTTGGCAGGATCAGCGA+GGG 0.665918 3_1:+87273095 None:intergenic
GATTATCGCTGCAATTAAGG+AGG 0.668758 3_1:-87272734 Msa0384190:CDS
CTTAGTCAAACTATGAGCAG+GGG 0.671198 3_1:-87270563 Msa0384190:three_prime_UTR
CACCTTTCACAAACACCGAG+TGG 0.683877 3_1:+87270984 None:intergenic
TATGGCGATGGAAATCCCAA+AGG 0.691099 3_1:-87271510 Msa0384190:intron
TGTTACTTTGGAGTTTGACG+CGG 0.700227 3_1:-87270809 Msa0384190:three_prime_UTR
AGATGTCAAGTACACAACTG+TGG 0.707472 3_1:-87270903 Msa0384190:CDS
TAGCCTTGGCAGGATCAGCG+AGG 0.707487 3_1:+87273094 None:intergenic
TGTCAAGTACACAACTGTGG+AGG 0.712034 3_1:-87270900 Msa0384190:CDS
GTCCTGGCTGAGCTAATGTG+GGG 0.743725 3_1:+87271571 None:intergenic
GGCTCCTATTCCAATCAACG+TGG 0.763745 3_1:-87271020 Msa0384190:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATAATATTAAGTTTGAAA+TGG - chr3_1:87270955-87270974 Msa0384190:CDS 10.0%
!!! GAATAGTTTTATTTTTTTTA+AGG - chr3_1:87271796-87271815 Msa0384190:intron 10.0%
!!! AAATAGTTTTATTTTTTTTG+AGG - chr3_1:87271829-87271848 Msa0384190:intron 10.0%
!!! AATAGTTTTATTTTTTTTGA+GGG - chr3_1:87271830-87271849 Msa0384190:intron 10.0%
!! AATCAAATTCATTAAAAAAG+TGG + chr3_1:87270824-87270843 None:intergenic 15.0%
!!! ATTTTTTAAGAAAAGTTGTT+AGG - chr3_1:87271416-87271435 Msa0384190:intron 15.0%
!!! TTTTTTAAGAAAAGTTGTTA+GGG - chr3_1:87271417-87271436 Msa0384190:intron 15.0%
!!! ATAGTTTTATTTTTTTTGAG+GGG - chr3_1:87271831-87271850 Msa0384190:intron 15.0%
!! AATATAATCAGAATATTAGT+TGG + chr3_1:87271889-87271908 None:intergenic 15.0%
!!! CTGATTATATTTTTCAATAA+AGG - chr3_1:87271898-87271917 Msa0384190:intron 15.0%
!!! TTTAATTTGAGAGTTTTTTA+GGG + chr3_1:87272026-87272045 None:intergenic 15.0%
!!! AACCTTTTTTGAATATTATT+CGG + chr3_1:87273113-87273132 None:intergenic 15.0%
!!! CTAAAGTTTTGAAATTATTG+AGG + chr3_1:87270763-87270782 None:intergenic 20.0%
!! CTCAATAATTTCAAAACTTT+AGG - chr3_1:87270761-87270780 Msa0384190:three_prime_UTR 20.0%
!! AAATTCATTAAAAAAGTGGA+AGG + chr3_1:87270820-87270839 None:intergenic 20.0%
!! AATGGTAATTAAATTATTGC+AGG - chr3_1:87270973-87270992 Msa0384190:CDS 20.0%
!! ATGGTAATTAAATTATTGCA+GGG - chr3_1:87270974-87270993 Msa0384190:CDS 20.0%
!! AAAAATGTGAAAAAGACAAA+GGG + chr3_1:87271402-87271421 None:intergenic 20.0%
!! AAAAAATGTGAAAAAGACAA+AGG + chr3_1:87271403-87271422 None:intergenic 20.0%
!! ATGCATCTAGTAATTTAATA+AGG - chr3_1:87271574-87271593 Msa0384190:CDS 20.0%
!! TTAATAAGGAGTTTACATTT+AGG - chr3_1:87271588-87271607 Msa0384190:CDS 20.0%
!! TAATAAGGAGTTTACATTTA+GGG - chr3_1:87271589-87271608 Msa0384190:CDS 20.0%
!! AAAAATAGTTAGTACTACTA+CGG - chr3_1:87271859-87271878 Msa0384190:intron 20.0%
!!! GTTTAATTTGAGAGTTTTTT+AGG + chr3_1:87272027-87272046 None:intergenic 20.0%
!!! TCATTTTCTTAAATCATTAC+CGG + chr3_1:87272364-87272383 None:intergenic 20.0%
!! TGAATGAATAAATGTTCTTT+GGG + chr3_1:87272609-87272628 None:intergenic 20.0%
!! TTGAATGAATAAATGTTCTT+TGG + chr3_1:87272610-87272629 None:intergenic 20.0%
!!! ACCTTTTTTGAATATTATTC+GGG + chr3_1:87273112-87273131 None:intergenic 20.0%
! GAATTGATCGAAAGTAATTA+AGG - chr3_1:87270569-87270588 Msa0384190:three_prime_UTR 25.0%
!! AAAAGCAAGATTTTGTTCAT+CGG - chr3_1:87270626-87270645 Msa0384190:three_prime_UTR 25.0%
! ATATCGTGTTTGTATATCAT+TGG - chr3_1:87270931-87270950 Msa0384190:CDS 25.0%
! TGGTAATTAAATTATTGCAG+GGG - chr3_1:87270975-87270994 Msa0384190:CDS 25.0%
! CATTTGGATTATGACTAAAT+GGG + chr3_1:87271262-87271281 None:intergenic 25.0%
! TCATTTGGATTATGACTAAA+TGG + chr3_1:87271263-87271282 None:intergenic 25.0%
! AAAATGTGAAAAAGACAAAG+GGG + chr3_1:87271401-87271420 None:intergenic 25.0%
! TTACATATTTCAAGACAAGT+TGG - chr3_1:87271512-87271531 Msa0384190:CDS 25.0%
! GGGACAAATATAAAATCAAA+TGG - chr3_1:87271609-87271628 Msa0384190:CDS 25.0%
! AGTATATGAAAATGTTTGCA+AGG + chr3_1:87271772-87271791 None:intergenic 25.0%
! TATTTCTTGTATATGTACAG+CGG - chr3_1:87272495-87272514 Msa0384190:intron 25.0%
! AATGAATAAATGTTCTTTGG+GGG + chr3_1:87272607-87272626 None:intergenic 25.0%
! GAATGAATAAATGTTCTTTG+GGG + chr3_1:87272608-87272627 None:intergenic 25.0%
!! ATATGAGTTGTTTGTTACTT+TGG - chr3_1:87273006-87273025 Msa0384190:intron 25.0%
!!! TTTCATTTGCTATGTAAACT+TGG - chr3_1:87273074-87273093 Msa0384190:CDS 25.0%
! ACCCGAATAATATTCAAAAA+AGG - chr3_1:87273108-87273127 Msa0384190:CDS 25.0%
! ACATTGAACAAATGGAATTT+AGG - chr3_1:87273289-87273308 Msa0384190:five_prime_UTR 25.0%
! CATTGAACAAATGGAATTTA+GGG - chr3_1:87273290-87273309 Msa0384190:five_prime_UTR 25.0%
! ATTGAACAAATGGAATTTAG+GGG - chr3_1:87273291-87273310 Msa0384190:five_prime_UTR 25.0%
!! GAAAAAACTCTAAAGCAGTT+TGG - chr3_1:87270539-87270558 Msa0384190:three_prime_UTR 30.0%
ATCGAAAGTAATTAAGGAAC+AGG - chr3_1:87270575-87270594 Msa0384190:three_prime_UTR 30.0%
TTCATTAAAAAAGTGGAAGG+AGG + chr3_1:87270817-87270836 None:intergenic 30.0%
GAGTTAATATTTCCAGCTAA+AGG + chr3_1:87270907-87270926 None:intergenic 30.0%
GGTAATTAAATTATTGCAGG+GGG - chr3_1:87270976-87270995 Msa0384190:CDS 30.0%
AAACCAGAAAAAAGGGTAAA+AGG + chr3_1:87271201-87271220 None:intergenic 30.0%
!!! TTTTACCCTTTTTTCTGGTT+TGG - chr3_1:87271200-87271219 Msa0384190:CDS 30.0%
!! ACAAGTTGGTAGTATCAATT+AGG - chr3_1:87271526-87271545 Msa0384190:CDS 30.0%
!! CAAGTTGGTAGTATCAATTA+GGG - chr3_1:87271527-87271546 Msa0384190:CDS 30.0%
!! AAGTTGGTAGTATCAATTAG+GGG - chr3_1:87271528-87271547 Msa0384190:CDS 30.0%
TCGTTTGAACACAAAGTTAA+AGG + chr3_1:87271989-87272008 None:intergenic 30.0%
!!! TTTGAGAGTTTTTTAGGGTT+CGG + chr3_1:87272021-87272040 None:intergenic 30.0%
!!! AGATTTTTTCCTTCGGAATT+TGG - chr3_1:87272095-87272114 Msa0384190:intron 30.0%
!!! GATTTTTTCCTTCGGAATTT+GGG - chr3_1:87272096-87272115 Msa0384190:intron 30.0%
! TATATGACGACTTTGTCTTT+TGG + chr3_1:87272299-87272318 None:intergenic 30.0%
AAAGACAAAGTCGTCATATA+TGG - chr3_1:87272299-87272318 Msa0384190:intron 30.0%
!! CATGTTAGTTTGTTACCTTT+GGG + chr3_1:87272335-87272354 None:intergenic 30.0%
!! ACATGTTAGTTTGTTACCTT+TGG + chr3_1:87272336-87272355 None:intergenic 30.0%
AAGGTAACAAACTAACATGT+TGG - chr3_1:87272336-87272355 Msa0384190:intron 30.0%
AGAATCTTATTCAATGTCCT+TGG + chr3_1:87272574-87272593 None:intergenic 30.0%
!!! TGGAAGAAAAGTTTTTTCGA+CGG + chr3_1:87272671-87272690 None:intergenic 30.0%
AAACTTCTCAAGGATATTCA+AGG - chr3_1:87272697-87272716 Msa0384190:intron 30.0%
! TATATTCCCTTTTATTGCAG+AGG - chr3_1:87272786-87272805 Msa0384190:CDS 30.0%
TGAAATAAGAAGCAACAAGT+AGG + chr3_1:87273059-87273078 None:intergenic 30.0%
!! ATTATTGAGGAGATTAGCCT+TGG + chr3_1:87270750-87270769 None:intergenic 35.0%
CAAAACTTTAGGCGTCAATT+TGG - chr3_1:87270772-87270791 Msa0384190:intron 35.0%
GGAGGAATATATTACGTACA+GGG + chr3_1:87270799-87270818 None:intergenic 35.0%
AGGAGGAATATATTACGTAC+AGG + chr3_1:87270800-87270819 None:intergenic 35.0%
TCTGTATGATCATGAGAACT+TGG - chr3_1:87271000-87271019 Msa0384190:CDS 35.0%
GAAGATTATCGCTGCAATTA+AGG - chr3_1:87271090-87271109 Msa0384190:intron 35.0%
AGAAGCCAAACCAGAAAAAA+GGG + chr3_1:87271208-87271227 None:intergenic 35.0%
AAGAAGCCAAACCAGAAAAA+AGG + chr3_1:87271209-87271228 None:intergenic 35.0%
! GACAAAGGGGTATACATATA+GGG + chr3_1:87271388-87271407 None:intergenic 35.0%
! AGACAAAGGGGTATACATAT+AGG + chr3_1:87271389-87271408 None:intergenic 35.0%
! TATCAATTAGGGGCATGTTT+AGG - chr3_1:87271538-87271557 Msa0384190:CDS 35.0%
CTCTCAAATTAAACTCTTGC+TGG - chr3_1:87272033-87272052 Msa0384190:intron 35.0%
CAAATTAAACTCTTGCTGGT+AGG - chr3_1:87272037-87272056 Msa0384190:intron 35.0%
!! GCTGGTAGGAATTTTAACAT+TGG - chr3_1:87272051-87272070 Msa0384190:intron 35.0%
CAGCAAAATCTGCATTTGAA+GGG - chr3_1:87272150-87272169 Msa0384190:intron 35.0%
! TTCAATAGCACGTCGAATTT+TGG + chr3_1:87272180-87272199 None:intergenic 35.0%
TTGCTAGACACATATGTGTA+GGG + chr3_1:87272215-87272234 None:intergenic 35.0%
TTCAACAAGGAAGATGACAT+TGG - chr3_1:87272520-87272539 Msa0384190:intron 35.0%
! CGAAAAAACTTTTCTTCCAG+AGG - chr3_1:87272672-87272691 Msa0384190:intron 35.0%
! AATACCACGTTGATTGGAAT+AGG + chr3_1:87272814-87272833 None:intergenic 35.0%
ATCGATAATACCACGTTGAT+TGG + chr3_1:87272820-87272839 None:intergenic 35.0%
! TTTGTGATTGAGCCATCTTT+TGG - chr3_1:87272877-87272896 Msa0384190:intron 35.0%
! TTGTGATTGAGCCATCTTTT+GGG - chr3_1:87272878-87272897 Msa0384190:intron 35.0%
TACTTCAAACGAACTGATCA+AGG + chr3_1:87272957-87272976 None:intergenic 35.0%
TTGTCAAAATGTTGCCTTCT+TGG - chr3_1:87273140-87273159 Msa0384190:CDS 35.0%
!! AATCTTGCTTTTCTCAGCCA+TGG + chr3_1:87270618-87270637 None:intergenic 40.0%
! AGCAAGATTTTGTTCATCGG+AGG - chr3_1:87270629-87270648 Msa0384190:three_prime_UTR 40.0%
! ATTTTGTTCATCGGAGGAAC+AGG - chr3_1:87270635-87270654 Msa0384190:three_prime_UTR 40.0%
CTTGGTGAAAGTAATCAAGC+AGG - chr3_1:87271018-87271037 Msa0384190:CDS 40.0%
AGTAATCAAGCAGGTAGATG+TGG - chr3_1:87271027-87271046 Msa0384190:intron 40.0%
GGTAGATGTGGTCATATCTA+CGG - chr3_1:87271039-87271058 Msa0384190:intron 40.0%
GATTATCGCTGCAATTAAGG+AGG - chr3_1:87271093-87271112 Msa0384190:intron 40.0%
! GTGCCTTTTACCCTTTTTTC+TGG - chr3_1:87271195-87271214 Msa0384190:CDS 40.0%
! AAGGGGTATACATATAGGGA+CGG + chr3_1:87271384-87271403 None:intergenic 40.0%
TCATGTACACCACACAAAAG+TGG - chr3_1:87271692-87271711 Msa0384190:CDS 40.0%
AAAGTGGACAGTCACAATCA+TGG - chr3_1:87271708-87271727 Msa0384190:CDS 40.0%
TGTGTCAAAGTCCAAATTGC+CGG + chr3_1:87271746-87271765 None:intergenic 40.0%
!!! TCCGCAGAGATTTTTTCCTT+CGG - chr3_1:87272088-87272107 Msa0384190:intron 40.0%
TCCGAAGGAAAAAATCTCTG+CGG + chr3_1:87272092-87272111 None:intergenic 40.0%
! CCTTCAAATGCAGATTTTGC+TGG + chr3_1:87272152-87272171 None:intergenic 40.0%
CCAGCAAAATCTGCATTTGA+AGG - chr3_1:87272149-87272168 Msa0384190:intron 40.0%
! AATTCGACGTGCTATTGAAG+CGG - chr3_1:87272181-87272200 Msa0384190:intron 40.0%
GTTGCTAGACACATATGTGT+AGG + chr3_1:87272216-87272235 None:intergenic 40.0%
TGTCTAGCAACTACTTTGCT+GGG - chr3_1:87272225-87272244 Msa0384190:intron 40.0%
! ATGACGACTTTGTCTTTTGG+AGG + chr3_1:87272296-87272315 None:intergenic 40.0%
AAAGTCGTCATATATGGCGA+TGG - chr3_1:87272305-87272324 Msa0384190:intron 40.0%
ACAAACTAACATGTTGGCAC+CGG - chr3_1:87272342-87272361 Msa0384190:intron 40.0%
AACATTCACCATTAGAGCTG+TGG - chr3_1:87272543-87272562 Msa0384190:intron 40.0%
TCCAGAGGATAAACTTCTCA+AGG - chr3_1:87272687-87272706 Msa0384190:intron 40.0%
TCCTTGAGAAGTTTATCCTC+TGG + chr3_1:87272691-87272710 None:intergenic 40.0%
TAGGAGCCTCTGCAATAAAA+GGG + chr3_1:87272795-87272814 None:intergenic 40.0%
ATAGGAGCCTCTGCAATAAA+AGG + chr3_1:87272796-87272815 None:intergenic 40.0%
! TGTGATTGAGCCATCTTTTG+GGG - chr3_1:87272879-87272898 Msa0384190:intron 40.0%
CAGTTGTGTACTTGACATCT+GGG + chr3_1:87272924-87272943 None:intergenic 40.0%
ACAGTTGTGTACTTGACATC+TGG + chr3_1:87272925-87272944 None:intergenic 40.0%
AGATGTCAAGTACACAACTG+TGG - chr3_1:87272924-87272943 Msa0384190:intron 40.0%
!! TGTTACTTTGGAGTTTGACG+CGG - chr3_1:87273018-87273037 Msa0384190:intron 40.0%
TAAGAAGCAACAAGTAGGAG+AGG + chr3_1:87273054-87273073 None:intergenic 40.0%
AAACTCAGATGACACCAAGA+AGG + chr3_1:87273157-87273176 None:intergenic 40.0%
GCTCATAGTTTGACTAAGCA+CGG + chr3_1:87273262-87273281 None:intergenic 40.0%
TGCTTAGTCAAACTATGAGC+AGG - chr3_1:87273262-87273281 Msa0384190:five_prime_UTR 40.0%
GCTTAGTCAAACTATGAGCA+GGG - chr3_1:87273263-87273282 Msa0384190:five_prime_UTR 40.0%
CTTAGTCAAACTATGAGCAG+GGG - chr3_1:87273264-87273283 Msa0384190:five_prime_UTR 40.0%
CAGGGGAAACATTGAACAAA+TGG - chr3_1:87273281-87273300 Msa0384190:five_prime_UTR 40.0%
ATCGGAGGAACAGGTTACAT+CGG - chr3_1:87270644-87270663 Msa0384190:three_prime_UTR 45.0%
GTAGAAGCAAGTGCAAAAGC+TGG - chr3_1:87270677-87270696 Msa0384190:three_prime_UTR 45.0%
!! TGGACATCCCACTTTTGCAT+TGG - chr3_1:87270697-87270716 Msa0384190:three_prime_UTR 45.0%
TCACGGACCAATGCAAAAGT+GGG + chr3_1:87270707-87270726 None:intergenic 45.0%
TTCACGGACCAATGCAAAAG+TGG + chr3_1:87270708-87270727 None:intergenic 45.0%
ACAGAGAGTGATCCTTTAGC+TGG - chr3_1:87270892-87270911 Msa0384190:CDS 45.0%
GATGTGGTCATATCTACGGT+AGG - chr3_1:87271043-87271062 Msa0384190:intron 45.0%
! TAAGGAGGCTGGTAACGTTA+AGG - chr3_1:87271108-87271127 Msa0384190:intron 45.0%
GGTATACATATAGGGACGGA+GGG + chr3_1:87271380-87271399 None:intergenic 45.0%
!! TGTGACTGTCCACTTTTGTG+TGG + chr3_1:87271704-87271723 None:intergenic 45.0%
TGGACAGTCACAATCATGGT+GGG - chr3_1:87271712-87271731 Msa0384190:CDS 45.0%
ACGTCATTCCCAAATTCCGA+AGG + chr3_1:87272107-87272126 None:intergenic 45.0%
! ATTTTGCTGGATCTACCGCA+TGG + chr3_1:87272139-87272158 None:intergenic 45.0%
GTGTCTAGCAACTACTTTGC+TGG - chr3_1:87272224-87272243 Msa0384190:intron 45.0%
!! TTTGTCTTTTGGAGGAGGTG+GGG + chr3_1:87272288-87272307 None:intergenic 45.0%
!! CTTTGTCTTTTGGAGGAGGT+GGG + chr3_1:87272289-87272308 None:intergenic 45.0%
!! ACTTTGTCTTTTGGAGGAGG+TGG + chr3_1:87272290-87272309 None:intergenic 45.0%
!! ACGACTTTGTCTTTTGGAGG+AGG + chr3_1:87272293-87272312 None:intergenic 45.0%
TATGGCGATGGAAATCCCAA+AGG - chr3_1:87272317-87272336 Msa0384190:intron 45.0%
ATGTACAGCGGTGTTCAACA+AGG - chr3_1:87272507-87272526 Msa0384190:intron 45.0%
! TGGATCATCCACAGCTCTAA+TGG + chr3_1:87272554-87272573 None:intergenic 45.0%
TTAGAGCTGTGGATGATCCA+AGG - chr3_1:87272554-87272573 Msa0384190:intron 45.0%
CCTTGTGGGAGAAGAAGATT+GGG - chr3_1:87272641-87272660 Msa0384190:intron 45.0%
GGTATTATCGATCCACCACT+CGG - chr3_1:87272828-87272847 Msa0384190:CDS 45.0%
TAAGCCTCAACCCCAAAAGA+TGG + chr3_1:87272892-87272911 None:intergenic 45.0%
TGTCAAGTACACAACTGTGG+AGG - chr3_1:87272927-87272946 Msa0384190:intron 45.0%
!! AATATAATATAATATAATTC+TGG - chr3_1:87271151-87271170 Msa0384190:CDS 5.0%
!! ATAAATAAATATTTATCATT+TGG + chr3_1:87271278-87271297 None:intergenic 5.0%
!!! ATTTAAATATTTTTTATTTG+AGG - chr3_1:87271648-87271667 Msa0384190:CDS 5.0%
TAACACACGCGTTGCATCCA+TGG - chr3_1:87270598-87270617 Msa0384190:three_prime_UTR 50.0%
!! TTGAGGAGATTAGCCTTGGC+AGG + chr3_1:87270746-87270765 None:intergenic 50.0%
CTTGATCCTCAATCAGAGCG+TGG + chr3_1:87271069-87271088 None:intergenic 50.0%
ATCGCTGCAATTAAGGAGGC+TGG - chr3_1:87271097-87271116 Msa0384190:intron 50.0%
GGGTATACATATAGGGACGG+AGG + chr3_1:87271381-87271400 None:intergenic 50.0%
GTGGACAGTCACAATCATGG+TGG - chr3_1:87271711-87271730 Msa0384190:CDS 50.0%
! AGTCACAATCATGGTGGGAG+TGG - chr3_1:87271717-87271736 Msa0384190:CDS 50.0%
! GTCACAATCATGGTGGGAGT+GGG - chr3_1:87271718-87271737 Msa0384190:CDS 50.0%
TGACGTTGATCGTGTCCATG+CGG - chr3_1:87272121-87272140 Msa0384190:intron 50.0%
TGTCCTGGCTGAGCTAATGT+GGG + chr3_1:87272260-87272279 None:intergenic 50.0%
TTGTCCTGGCTGAGCTAATG+TGG + chr3_1:87272261-87272280 None:intergenic 50.0%
! TCAACGAGCTTGTAGCCTTG+TGG - chr3_1:87272626-87272645 Msa0384190:intron 50.0%
!! CAACGAGCTTGTAGCCTTGT+GGG - chr3_1:87272627-87272646 Msa0384190:intron 50.0%
GCCTTGTGGGAGAAGAAGAT+TGG - chr3_1:87272640-87272659 Msa0384190:intron 50.0%
CCCAATCTTCTTCTCCCACA+AGG + chr3_1:87272644-87272663 None:intergenic 50.0%
GGCTCCTATTCCAATCAACG+TGG - chr3_1:87272807-87272826 Msa0384190:CDS 50.0%
CTTTCACAAACACCGAGTGG+TGG + chr3_1:87272843-87272862 None:intergenic 50.0%
!! CACCACTCGGTGTTTGTGAA+AGG - chr3_1:87272841-87272860 Msa0384190:intron 50.0%
CACCTTTCACAAACACCGAG+TGG + chr3_1:87272846-87272865 None:intergenic 50.0%
!!! TGAGCCATCTTTTGGGGTTG+AGG - chr3_1:87272885-87272904 Msa0384190:intron 50.0%
! GATCAGCGAGGGTGGATTCA+CGG + chr3_1:87270724-87270743 None:intergenic 55.0%
! ATCATGGTGGGAGTGGGATC+CGG - chr3_1:87271724-87271743 Msa0384190:CDS 55.0%
! CGACGTGCTATTGAAGCGGA+AGG - chr3_1:87272185-87272204 Msa0384190:intron 55.0%
GTCCTGGCTGAGCTAATGTG+GGG + chr3_1:87272259-87272278 None:intergenic 55.0%
AGCCTTGGCAGGATCAGCGA+GGG + chr3_1:87270735-87270754 None:intergenic 60.0%
! TAGCCTTGGCAGGATCAGCG+AGG + chr3_1:87270736-87270755 None:intergenic 60.0%
GGTAGGCCACGCTCTGATTG+AGG - chr3_1:87271060-87271079 Msa0384190:intron 60.0%
GGGAGTGGGATCCGGCAATT+TGG - chr3_1:87271732-87271751 Msa0384190:CDS 60.0%
CTCCCCACATTAGCTCAGCC+AGG - chr3_1:87272254-87272273 Msa0384190:intron 60.0%
CTTGGCAGGATCAGCGAGGG+TGG + chr3_1:87270732-87270751 None:intergenic 65.0%
CACCCTCGCTGATCCTGCCA+AGG - chr3_1:87270730-87270749 Msa0384190:three_prime_UTR 65.0%
GGAGGTGGGGCGAATTGTCC+TGG + chr3_1:87272275-87272294 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 87270516 87273333 87270516 ID=Msa0384190;Name=Msa0384190
chr3_1 mRNA 87270516 87273333 87270516 ID=Msa0384190-mRNA-1;Parent=Msa0384190;Name=Msa0384190-mRNA-1;_AED=0.28;_eAED=0.28;_QI=101|1|1|1|0.8|0.66|6|339|310
chr3_1 exon 87270516 87270782 87270516 ID=Msa0384190-mRNA-1:exon:23292;Parent=Msa0384190-mRNA-1
chr3_1 exon 87270805 87271043 87270805 ID=Msa0384190-mRNA-1:exon:23291;Parent=Msa0384190-mRNA-1
chr3_1 exon 87271131 87271334 87271131 ID=Msa0384190-mRNA-1:exon:23290;Parent=Msa0384190-mRNA-1
chr3_1 exon 87271511 87271754 87271511 ID=Msa0384190-mRNA-1:exon:23289;Parent=Msa0384190-mRNA-1
chr3_1 exon 87272720 87272854 87272720 ID=Msa0384190-mRNA-1:exon:23288;Parent=Msa0384190-mRNA-1
chr3_1 exon 87273050 87273333 87273050 ID=Msa0384190-mRNA-1:exon:23287;Parent=Msa0384190-mRNA-1
chr3_1 five_prime_UTR 87273233 87273333 87273233 ID=Msa0384190-mRNA-1:five_prime_utr;Parent=Msa0384190-mRNA-1
chr3_1 CDS 87273050 87273232 87273050 ID=Msa0384190-mRNA-1:cds;Parent=Msa0384190-mRNA-1
chr3_1 CDS 87272720 87272854 87272720 ID=Msa0384190-mRNA-1:cds;Parent=Msa0384190-mRNA-1
chr3_1 CDS 87271511 87271754 87271511 ID=Msa0384190-mRNA-1:cds;Parent=Msa0384190-mRNA-1
chr3_1 CDS 87271131 87271334 87271131 ID=Msa0384190-mRNA-1:cds;Parent=Msa0384190-mRNA-1
chr3_1 CDS 87270877 87271043 87270877 ID=Msa0384190-mRNA-1:cds;Parent=Msa0384190-mRNA-1
chr3_1 three_prime_UTR 87270805 87270876 87270805 ID=Msa0384190-mRNA-1:three_prime_utr;Parent=Msa0384190-mRNA-1
chr3_1 three_prime_UTR 87270516 87270782 87270516 ID=Msa0384190-mRNA-1:three_prime_utr;Parent=Msa0384190-mRNA-1
Gene Sequence

>Msa0384190

ATGGCTGAGAAAAGCAAGATTTTGTTCATCGGAGGAACAGGTTACATCGGAAAACATATAGTAGAAGCAAGTGCAAAAGCTGGACATCCCACTTTTGCATTGGTCCGTGAATCCACCCTCGCTGATCCTGCCAAGGCTAATCTCCTCAATAATTTCAAAACTTTAGGCGTCAATTTGGTCCCTGGGGATCTGTATGATCATGAGAACTTGGTGAAAGTAATCAAGCAGGTAGATGTGGTCATATCTACGGTAGGCCACGCTCTGATTGAGGATCAAGTGAAGATTATCGCTGCAATTAAGGAGGCTGGTAACGTTAAGAGATTTTTTCCTTCGGAATTTGGGAATGACGTTGATCGTGTCCATGCGGTAGATCCAGCAAAATCTGCATTTGAAGGGAAAGCCAAAATTCGACGTGCTATTGAAGCGGAAGGCATACCCTACACATATGTGTCTAGCAACTACTTTGCTGGGTATTTTCTCCCCACATTAGCTCAGCCAGGACAATTCGCCCCACCTCCTCCAAAAGACAAAGTCGTCATATATGGCGATGGAAATCCCAAAGCGGTGTTCAACAAGGAAGATGACATTGGAACATTCACCATTAGAGCTGTGGATGATCCAAGGACATTGAATAAGATTCTCTACATAAAGCCCCCAAAGAACATTTATTCATTCAACGAGCTTGTAGCCTTGTGGGAGAAGAAGATTGGGAAGACCGTCGAAAAAACTTTTCTTCCAGAGGATAAACTTCTCAAGGATATTCAAGAGGCTCCTATTCCAATCAACGTGGTATTATCGATCCACCACTCGGTGTTTGTGAAAGGTGATCACACAAACTTTGTGATTGAGCCATCTTTTGGGGTTGAGGCTTATGAGTTGTACCCAGATGTCAAGTACACAACTGTGGAGGAATACCTTGATCAGTTCGTTTGA
Protein sequence

>Msa0384190

MAEKSKILFIGGTGYIGKHIVEASAKAGHPTFALVRESTLADPAKANLLNNFKTLGVNLVPGDLYDHENLVKVIKQVDVVISTVGHALIEDQVKIIAAIKEAGNVKRFFPSEFGNDVDRVHAVDPAKSAFEGKAKIRRAIEAEGIPYTYVSSNYFAGYFLPTLAQPGQFAPPPPKDKVVIYGDGNPKAVFNKEDDIGTFTIRAVDDPRTLNKILYIKPPKNIYSFNELVALWEKKIGKTVEKTFLPEDKLLKDIQEAPIPINVVLSIHHSVFVKGDHTNFVIEPSFGVEAYELYPDVKYTTVEEYLDQFV*