AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0464390

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0464390 MtrunA17_Chr3g0115421 99.329 149 1 0 1 149 1 149 7.87e-108 303
Msa0464390 MtrunA17_Chr8g0382721 62.745 153 53 2 1 149 1 153 2.58e-59 181
Msa0464390 MtrunA17_Chr3g0124411 60.390 154 54 3 1 149 1 152 1.72e-58 178
Msa0464390 MtrunA17_Chr4g0036931 51.613 155 65 3 5 149 2 156 2.91e-49 155
Msa0464390 MtrunA17_Chr1g0160201 51.020 147 69 2 5 149 2 147 7.50e-48 151
Msa0464390 MtrunA17_Chr3g0145621 47.436 156 75 2 1 149 1 156 9.58e-48 151
Msa0464390 MtrunA17_Chr5g0405811 48.077 156 70 3 5 149 2 157 3.29e-45 145
Msa0464390 MtrunA17_Chr5g0407821 46.897 145 76 1 5 149 2 145 1.59e-44 143
Msa0464390 MtrunA17_Chr6g0448861 44.805 154 76 3 5 149 2 155 4.73e-43 139
Msa0464390 MtrunA17_Chr7g0251491 43.537 147 79 3 5 149 26 170 6.88e-38 127
Msa0464390 MtrunA17_Chr5g0422891 38.095 147 88 2 5 149 2 147 1.25e-32 112
Msa0464390 MtrunA17_Chr2g0325601 33.333 138 73 3 30 149 6 142 8.99e-20 79.7
Msa0464390 MtrunA17_Chr1g0189221 48.148 81 38 1 26 102 53 133 2.76e-19 79.7
Msa0464390 MtrunA17_Chr6g0471231 38.168 131 66 4 33 149 11 140 7.58e-19 77.4
Msa0464390 MtrunA17_Chr1g0198341 50.000 66 33 0 25 90 3 68 6.72e-16 68.2
Msa0464390 MtrunA17_Chr7g0218981 43.939 66 37 0 25 90 3 68 3.36e-15 67.8
Msa0464390 MtrunA17_Chr6g0460361 37.963 108 56 4 52 149 4 110 5.44e-14 63.9
Msa0464390 MtrunA17_Chr7g0272571 43.077 65 37 0 27 91 34 98 6.72e-13 64.7
Msa0464390 MtrunA17_Chr1g0192131 39.744 78 46 1 15 91 4 81 9.60e-13 64.3
Msa0464390 MtrunA17_Chr7g0269081 40.000 65 39 0 26 90 22 86 1.29e-12 63.5
Msa0464390 MtrunA17_Chr1g0191671 44.615 65 36 0 26 90 10 74 2.00e-12 63.5
Msa0464390 MtrunA17_Chr1g0208301 43.077 65 37 0 26 90 13 77 2.41e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0464390 AT4G08570.1 76.000 150 35 1 1 149 1 150 4.39e-81 236
Msa0464390 AT4G39700.1 60.127 158 54 3 1 149 1 158 3.51e-57 176
Msa0464390 AT1G22990.1 55.921 152 59 3 5 149 2 152 6.52e-57 175
Msa0464390 AT1G22990.2 53.642 151 59 4 5 149 2 147 5.63e-53 164
Msa0464390 AT1G71050.1 51.974 152 70 3 1 149 1 152 2.51e-48 153
Msa0464390 AT4G38580.1 48.684 152 71 3 5 149 2 153 1.50e-45 146
Msa0464390 AT4G38580.2 48.684 152 71 3 5 149 2 153 1.50e-45 146
Msa0464390 AT5G66110.3 50.345 145 67 3 10 149 3 147 7.51e-45 144
Msa0464390 AT5G66110.1 50.345 145 67 3 10 149 3 147 7.51e-45 144
Msa0464390 AT5G66110.2 53.719 121 51 3 34 149 1 121 6.26e-40 130
Msa0464390 AT4G35060.1 41.447 152 82 3 5 149 2 153 3.72e-38 127
Msa0464390 AT5G17450.1 44.737 152 73 4 5 149 2 149 1.34e-36 123
Msa0464390 AT5G17450.2 46.078 102 47 3 52 149 8 105 2.60e-24 90.9
Msa0464390 AT2G18196.1 37.594 133 74 3 26 149 46 178 1.02e-23 91.3
Msa0464390 AT1G06330.1 35.135 148 71 3 27 149 3 150 2.65e-22 87.0
Msa0464390 AT2G18196.2 36.000 125 71 3 34 149 1 125 6.82e-20 80.1
Msa0464390 AT4G10465.1 35.000 140 82 3 19 149 44 183 4.10e-19 79.7
Msa0464390 AT3G56891.1 31.333 150 74 4 28 149 18 166 2.24e-18 77.4
Msa0464390 AT1G29100.2 34.014 147 74 4 25 149 2 147 6.94e-18 75.5
Msa0464390 AT3G56891.2 31.250 144 70 4 34 149 1 143 2.10e-17 74.3
Msa0464390 AT1G29100.1 33.803 142 71 4 30 149 1 141 8.03e-17 72.4
Msa0464390 AT1G66240.1 47.826 69 36 0 22 90 29 97 5.08e-16 69.7
Msa0464390 AT3G56240.3 46.970 66 35 0 26 91 3 68 2.86e-15 68.2
Msa0464390 AT3G56240.1 46.970 66 35 0 26 91 3 68 2.86e-15 68.2
Msa0464390 AT5G27690.1 45.161 62 34 0 30 91 34 95 7.73e-14 67.8
Msa0464390 AT1G56210.1 43.478 69 39 0 26 94 40 108 1.71e-13 66.6
Msa0464390 AT3G48970.1 36.879 141 71 6 25 149 2 140 3.69e-13 63.2
Msa0464390 AT3G48970.2 37.410 139 69 6 27 149 2 138 5.12e-13 62.8
Msa0464390 AT3G48970.3 37.410 139 69 6 27 149 2 138 5.12e-13 62.8
Msa0464390 AT1G66240.2 49.123 57 29 0 34 90 1 57 2.30e-12 58.9
Msa0464390 AT3G56240.2 44.828 58 32 0 34 91 1 58 4.61e-12 59.3

Find 47 sgRNAs with CRISPR-Local

Find 176 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATTAATTTCTCACCCCTTA+TGG 0.244698 3_3:+64291231 Msa0464390:three_prime_UTR
AAGGTTAAGCATGTCCTTTC+TGG 0.256831 3_3:+64290446 Msa0464390:CDS
GCAACCTTTCTAACCATATT+AGG 0.261201 3_3:-64291100 None:intergenic
CATGTCCTTTCTGGTGTTAA+AGG 0.262150 3_3:+64290455 Msa0464390:CDS
TCATAGGCTTGTGAAATATA+AGG 0.273413 3_3:-64291064 None:intergenic
GATAATGATGATTGATTGTT+AGG 0.273640 3_3:+64291431 Msa0464390:three_prime_UTR
GCAGCTTTCAACACTTTCTT+TGG 0.279745 3_3:-64290980 None:intergenic
GTAAATCAGACAAGTATTCT+AGG 0.300083 3_3:-64290340 None:intergenic
TGAGGAGAAAATAATGTAAT+AGG 0.320083 3_3:+64291265 Msa0464390:three_prime_UTR
CAAGTATGAGAACAAAATTA+TGG 0.356872 3_3:-64290158 None:intergenic
TAAGAAAGCACCTCCTAATA+TGG 0.356938 3_3:+64291087 Msa0464390:CDS
GCCTTATGTTCCATACACTA+TGG 0.372587 3_3:+64291033 Msa0464390:CDS
AGGAGGTGCTTTCTTATCAT+AGG 0.375360 3_3:-64291080 None:intergenic
TACATAATAGAACAAGCATT+TGG 0.390614 3_3:-64291190 None:intergenic
GAGATAGAGATATGTGATAG+AGG 0.407764 3_3:+64291307 Msa0464390:three_prime_UTR
GATTGTTAGGGTGGTTGAAA+TGG 0.420007 3_3:+64291444 Msa0464390:three_prime_UTR
TCAAAGTGTGATTACCATAA+GGG 0.421588 3_3:-64291245 None:intergenic
ATTCAAGAAATAAACTACTT+TGG 0.423174 3_3:-64290249 None:intergenic
CTCAAAGTGTGATTACCATA+AGG 0.439579 3_3:-64291246 None:intergenic
CATAGGCTTGTGAAATATAA+GGG 0.443566 3_3:-64291063 None:intergenic
CAAAGAAGAAGGTTGAGCTT+TGG 0.454326 3_3:+64291011 Msa0464390:CDS
ACCTCCTAATATGGTTAGAA+AGG 0.456268 3_3:+64291096 Msa0464390:CDS
AATTGACTGATTGAAGTGTA+TGG 0.461832 3_3:-64290119 None:intergenic
ACCATAGTGTATGGAACATA+AGG 0.464116 3_3:-64291034 None:intergenic
AACATAGCTATCATCAAAAG+TGG 0.479724 3_3:-64291146 None:intergenic
GGGTGAGCCACCATAGTGTA+TGG 0.494549 3_3:-64291043 None:intergenic
CTTATGGTAATCACACTTTG+AGG 0.514753 3_3:+64291247 Msa0464390:three_prime_UTR
TGTATCTCTTAAAATCAGAA+TGG 0.514900 3_3:+64290403 Msa0464390:CDS
TGCTCAATCAACAAAGAAGA+AGG 0.535682 3_3:+64291000 Msa0464390:CDS
ATAATGATGATTGATTGTTA+GGG 0.541805 3_3:+64291432 Msa0464390:three_prime_UTR
AGGAGCTAAGAAAGTGGATG+TGG 0.542853 3_3:+64290916 Msa0464390:intron
AAGTGTATGGAAATATAGAA+AGG 0.553419 3_3:-64290106 None:intergenic
TAAATCAGACAAGTATTCTA+GGG 0.567273 3_3:-64290339 None:intergenic
CTGTGAAGGTTGTGCTCGCA+AGG 0.570301 3_3:+64290427 Msa0464390:CDS
ATGATGATTGATTGTTAGGG+TGG 0.571522 3_3:+64291435 Msa0464390:three_prime_UTR
ACCTTTCTAACCATATTAGG+AGG 0.574085 3_3:-64291097 None:intergenic
CAGCAGAAAGTAACAGTGAG+TGG 0.576096 3_3:+64290947 Msa0464390:CDS
TTGAACAGGAGCTAAGAAAG+TGG 0.579198 3_3:+64290910 Msa0464390:intron
ATAGAGATATGTGATAGAGG+AGG 0.586370 3_3:+64291310 Msa0464390:three_prime_UTR
CAAAGTGTGATTACCATAAG+GGG 0.600902 3_3:-64291244 None:intergenic
CTTGAACAAACTAGAGTGGT+CGG 0.607671 3_3:+64290177 Msa0464390:five_prime_UTR
AAAATCAGAATGGACTGTGA+AGG 0.610949 3_3:+64290413 Msa0464390:CDS
TTATGTTCCATACACTATGG+TGG 0.612160 3_3:+64291036 Msa0464390:CDS
AAGTACCTTTAACACCAGAA+AGG 0.613905 3_3:-64290460 None:intergenic
CATACTTGAACAAACTAGAG+TGG 0.652576 3_3:+64290173 Msa0464390:five_prime_UTR
TGGTTGAAATGGAACACACT+TGG 0.653186 3_3:+64291455 Msa0464390:three_prime_UTR
AGTAAGCTAGAATCATTGTG+AGG 0.719657 3_3:+64290278 Msa0464390:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATTATATATATATAGACA+TGG - chr3_3:64290222-64290241 None:intergenic 10.0%
!!! AATAATAATATAATTTTGCA+TGG + chr3_3:64291388-64291407 Msa0464390:three_prime_UTR 10.0%
!! TTATTATATATATATAGACA+TGG - chr3_3:64290222-64290241 None:intergenic 10.0%
!!! AATAATAATATAATTTTGCA+TGG + chr3_3:64291388-64291407 Msa0464390:three_prime_UTR 10.0%
!!! TAATGCATAATTTTAAATTG+TGG - chr3_3:64290651-64290670 None:intergenic 15.0%
!!! ATTGTTTTTAGTTCATTTAT+TGG + chr3_3:64290872-64290891 Msa0464390:intron 15.0%
!!! AATTAATTTGAAATTTGAAC+AGG + chr3_3:64290896-64290915 Msa0464390:intron 15.0%
!!! TAATGCATAATTTTAAATTG+TGG - chr3_3:64290651-64290670 None:intergenic 15.0%
!!! ATTGTTTTTAGTTCATTTAT+TGG + chr3_3:64290872-64290891 Msa0464390:intron 15.0%
!!! AATTAATTTGAAATTTGAAC+AGG + chr3_3:64290896-64290915 Msa0464390:intron 15.0%
!! ATTCAAGAAATAAACTACTT+TGG - chr3_3:64290252-64290271 None:intergenic 20.0%
!!! TATTGTTTTTAGTTCATTTG+AGG + chr3_3:64290669-64290688 Msa0464390:intron 20.0%
!!! ATTGTTTTTAGTTCATTTGA+GGG + chr3_3:64290670-64290689 Msa0464390:intron 20.0%
!! ATAATGATGATTGATTGTTA+GGG + chr3_3:64291432-64291451 Msa0464390:three_prime_UTR 20.0%
!! ATTCAAGAAATAAACTACTT+TGG - chr3_3:64290252-64290271 None:intergenic 20.0%
!!! TATTGTTTTTAGTTCATTTG+AGG + chr3_3:64290669-64290688 Msa0464390:intron 20.0%
!!! ATTGTTTTTAGTTCATTTGA+GGG + chr3_3:64290670-64290689 Msa0464390:intron 20.0%
!! ATAATGATGATTGATTGTTA+GGG + chr3_3:64291432-64291451 Msa0464390:three_prime_UTR 20.0%
! AAGTGTATGGAAATATAGAA+AGG - chr3_3:64290109-64290128 None:intergenic 25.0%
! CAAGTATGAGAACAAAATTA+TGG - chr3_3:64290161-64290180 None:intergenic 25.0%
! TAAATCAGACAAGTATTCTA+GGG - chr3_3:64290342-64290361 None:intergenic 25.0%
! TGTATCTCTTAAAATCAGAA+TGG + chr3_3:64290403-64290422 Msa0464390:CDS 25.0%
!!! TTGTTTTTAGTTCATTTGAG+GGG + chr3_3:64290671-64290690 Msa0464390:intron 25.0%
! CCTCTTGTGTAAAAAAAAAT+GGG + chr3_3:64290764-64290783 Msa0464390:intron 25.0%
! CTCTTGTGTAAAAAAAAATG+GGG + chr3_3:64290765-64290784 Msa0464390:intron 25.0%
! TACATAATAGAACAAGCATT+TGG - chr3_3:64291193-64291212 None:intergenic 25.0%
! TGAGGAGAAAATAATGTAAT+AGG + chr3_3:64291265-64291284 Msa0464390:three_prime_UTR 25.0%
! GATAATGATGATTGATTGTT+AGG + chr3_3:64291431-64291450 Msa0464390:three_prime_UTR 25.0%
! AAGTGTATGGAAATATAGAA+AGG - chr3_3:64290109-64290128 None:intergenic 25.0%
! CAAGTATGAGAACAAAATTA+TGG - chr3_3:64290161-64290180 None:intergenic 25.0%
! TAAATCAGACAAGTATTCTA+GGG - chr3_3:64290342-64290361 None:intergenic 25.0%
! TGTATCTCTTAAAATCAGAA+TGG + chr3_3:64290403-64290422 Msa0464390:CDS 25.0%
!!! TTGTTTTTAGTTCATTTGAG+GGG + chr3_3:64290671-64290690 Msa0464390:intron 25.0%
! CCTCTTGTGTAAAAAAAAAT+GGG + chr3_3:64290764-64290783 Msa0464390:intron 25.0%
! CTCTTGTGTAAAAAAAAATG+GGG + chr3_3:64290765-64290784 Msa0464390:intron 25.0%
! TACATAATAGAACAAGCATT+TGG - chr3_3:64291193-64291212 None:intergenic 25.0%
! TGAGGAGAAAATAATGTAAT+AGG + chr3_3:64291265-64291284 Msa0464390:three_prime_UTR 25.0%
! GATAATGATGATTGATTGTT+AGG + chr3_3:64291431-64291450 Msa0464390:three_prime_UTR 25.0%
AATTGACTGATTGAAGTGTA+TGG - chr3_3:64290122-64290141 None:intergenic 30.0%
AGCAAGAATAAGCAGAAAAA+TGG + chr3_3:64290304-64290323 Msa0464390:exon 30.0%
GCAAGAATAAGCAGAAAAAT+GGG + chr3_3:64290305-64290324 Msa0464390:exon 30.0%
GTAAATCAGACAAGTATTCT+AGG - chr3_3:64290343-64290362 None:intergenic 30.0%
! AAACGAGGCAATGATTTTTA+AGG - chr3_3:64290516-64290535 None:intergenic 30.0%
!! CATTGCCTCGTTTTAAATTA+CGG + chr3_3:64290523-64290542 Msa0464390:intron 30.0%
! ACTTTTATATTGCGCAATTG+TGG + chr3_3:64290618-64290637 Msa0464390:intron 30.0%
GCCTCTTGTGTAAAAAAAAA+TGG + chr3_3:64290763-64290782 Msa0464390:intron 30.0%
!!! CCCATTTTTTTTTACACAAG+AGG - chr3_3:64290767-64290786 None:intergenic 30.0%
GTGTAAAAAAAAATGGGGTA+AGG + chr3_3:64290770-64290789 Msa0464390:intron 30.0%
CATAGGCTTGTGAAATATAA+GGG - chr3_3:64291066-64291085 None:intergenic 30.0%
TCATAGGCTTGTGAAATATA+AGG - chr3_3:64291067-64291086 None:intergenic 30.0%
!! AAAAGTGGACTCTTTTATGT+TGG - chr3_3:64291134-64291153 None:intergenic 30.0%
AACATAGCTATCATCAAAAG+TGG - chr3_3:64291149-64291168 None:intergenic 30.0%
TCAAAGTGTGATTACCATAA+GGG - chr3_3:64291248-64291267 None:intergenic 30.0%
AATTGACTGATTGAAGTGTA+TGG - chr3_3:64290122-64290141 None:intergenic 30.0%
AGCAAGAATAAGCAGAAAAA+TGG + chr3_3:64290304-64290323 Msa0464390:exon 30.0%
GCAAGAATAAGCAGAAAAAT+GGG + chr3_3:64290305-64290324 Msa0464390:exon 30.0%
GTAAATCAGACAAGTATTCT+AGG - chr3_3:64290343-64290362 None:intergenic 30.0%
! AAACGAGGCAATGATTTTTA+AGG - chr3_3:64290516-64290535 None:intergenic 30.0%
!! CATTGCCTCGTTTTAAATTA+CGG + chr3_3:64290523-64290542 Msa0464390:intron 30.0%
! ACTTTTATATTGCGCAATTG+TGG + chr3_3:64290618-64290637 Msa0464390:intron 30.0%
GCCTCTTGTGTAAAAAAAAA+TGG + chr3_3:64290763-64290782 Msa0464390:intron 30.0%
!!! CCCATTTTTTTTTACACAAG+AGG - chr3_3:64290767-64290786 None:intergenic 30.0%
GTGTAAAAAAAAATGGGGTA+AGG + chr3_3:64290770-64290789 Msa0464390:intron 30.0%
CATAGGCTTGTGAAATATAA+GGG - chr3_3:64291066-64291085 None:intergenic 30.0%
TCATAGGCTTGTGAAATATA+AGG - chr3_3:64291067-64291086 None:intergenic 30.0%
!! AAAAGTGGACTCTTTTATGT+TGG - chr3_3:64291134-64291153 None:intergenic 30.0%
AACATAGCTATCATCAAAAG+TGG - chr3_3:64291149-64291168 None:intergenic 30.0%
TCAAAGTGTGATTACCATAA+GGG - chr3_3:64291248-64291267 None:intergenic 30.0%
CATACTTGAACAAACTAGAG+TGG + chr3_3:64290173-64290192 Msa0464390:five_prime_UTR 35.0%
AGTAAGCTAGAATCATTGTG+AGG + chr3_3:64290278-64290297 Msa0464390:five_prime_UTR 35.0%
!! AAAATCAGAATGGACTGTGA+AGG + chr3_3:64290413-64290432 Msa0464390:CDS 35.0%
AAGTACCTTTAACACCAGAA+AGG - chr3_3:64290463-64290482 None:intergenic 35.0%
! TGCAACCGTAATTTAAAACG+AGG - chr3_3:64290531-64290550 None:intergenic 35.0%
!!! CTACAGCAGTCGATATTTTT+TGG - chr3_3:64290587-64290606 None:intergenic 35.0%
TGCTCAATCAACAAAGAAGA+AGG + chr3_3:64291000-64291019 Msa0464390:CDS 35.0%
ACCATAGTGTATGGAACATA+AGG - chr3_3:64291037-64291056 None:intergenic 35.0%
TTATGTTCCATACACTATGG+TGG + chr3_3:64291036-64291055 Msa0464390:CDS 35.0%
!! TAAGAAAGCACCTCCTAATA+TGG + chr3_3:64291087-64291106 Msa0464390:CDS 35.0%
ACCTCCTAATATGGTTAGAA+AGG + chr3_3:64291096-64291115 Msa0464390:CDS 35.0%
ACCTTTCTAACCATATTAGG+AGG - chr3_3:64291100-64291119 None:intergenic 35.0%
GCAACCTTTCTAACCATATT+AGG - chr3_3:64291103-64291122 None:intergenic 35.0%
GATTAATTTCTCACCCCTTA+TGG + chr3_3:64291231-64291250 Msa0464390:three_prime_UTR 35.0%
CAAAGTGTGATTACCATAAG+GGG - chr3_3:64291247-64291266 None:intergenic 35.0%
CTCAAAGTGTGATTACCATA+AGG - chr3_3:64291249-64291268 None:intergenic 35.0%
CTTATGGTAATCACACTTTG+AGG + chr3_3:64291247-64291266 Msa0464390:three_prime_UTR 35.0%
GAGATAGAGATATGTGATAG+AGG + chr3_3:64291307-64291326 Msa0464390:three_prime_UTR 35.0%
ATAGAGATATGTGATAGAGG+AGG + chr3_3:64291310-64291329 Msa0464390:three_prime_UTR 35.0%
ATGATGATTGATTGTTAGGG+TGG + chr3_3:64291435-64291454 Msa0464390:three_prime_UTR 35.0%
CATACTTGAACAAACTAGAG+TGG + chr3_3:64290173-64290192 Msa0464390:five_prime_UTR 35.0%
AGTAAGCTAGAATCATTGTG+AGG + chr3_3:64290278-64290297 Msa0464390:five_prime_UTR 35.0%
!! AAAATCAGAATGGACTGTGA+AGG + chr3_3:64290413-64290432 Msa0464390:CDS 35.0%
AAGTACCTTTAACACCAGAA+AGG - chr3_3:64290463-64290482 None:intergenic 35.0%
! TGCAACCGTAATTTAAAACG+AGG - chr3_3:64290531-64290550 None:intergenic 35.0%
!!! CTACAGCAGTCGATATTTTT+TGG - chr3_3:64290587-64290606 None:intergenic 35.0%
TGCTCAATCAACAAAGAAGA+AGG + chr3_3:64291000-64291019 Msa0464390:CDS 35.0%
ACCATAGTGTATGGAACATA+AGG - chr3_3:64291037-64291056 None:intergenic 35.0%
TTATGTTCCATACACTATGG+TGG + chr3_3:64291036-64291055 Msa0464390:CDS 35.0%
!! TAAGAAAGCACCTCCTAATA+TGG + chr3_3:64291087-64291106 Msa0464390:CDS 35.0%
ACCTCCTAATATGGTTAGAA+AGG + chr3_3:64291096-64291115 Msa0464390:CDS 35.0%
ACCTTTCTAACCATATTAGG+AGG - chr3_3:64291100-64291119 None:intergenic 35.0%
GCAACCTTTCTAACCATATT+AGG - chr3_3:64291103-64291122 None:intergenic 35.0%
GATTAATTTCTCACCCCTTA+TGG + chr3_3:64291231-64291250 Msa0464390:three_prime_UTR 35.0%
CAAAGTGTGATTACCATAAG+GGG - chr3_3:64291247-64291266 None:intergenic 35.0%
CTCAAAGTGTGATTACCATA+AGG - chr3_3:64291249-64291268 None:intergenic 35.0%
CTTATGGTAATCACACTTTG+AGG + chr3_3:64291247-64291266 Msa0464390:three_prime_UTR 35.0%
GAGATAGAGATATGTGATAG+AGG + chr3_3:64291307-64291326 Msa0464390:three_prime_UTR 35.0%
ATAGAGATATGTGATAGAGG+AGG + chr3_3:64291310-64291329 Msa0464390:three_prime_UTR 35.0%
ATGATGATTGATTGTTAGGG+TGG + chr3_3:64291435-64291454 Msa0464390:three_prime_UTR 35.0%
CTTGAACAAACTAGAGTGGT+CGG + chr3_3:64290177-64290196 Msa0464390:five_prime_UTR 40.0%
! AAGCAGAAAAATGGGAGTAC+AGG + chr3_3:64290313-64290332 Msa0464390:exon 40.0%
! AGCAGAAAAATGGGAGTACA+GGG + chr3_3:64290314-64290333 Msa0464390:exon 40.0%
AAGGTTAAGCATGTCCTTTC+TGG + chr3_3:64290446-64290465 Msa0464390:CDS 40.0%
! CATGTCCTTTCTGGTGTTAA+AGG + chr3_3:64290455-64290474 Msa0464390:CDS 40.0%
ATTGTCGTCATGTGACTGAA+AGG + chr3_3:64290716-64290735 Msa0464390:intron 40.0%
TTACACAAGAGGCTGTTTAC+AGG - chr3_3:64290756-64290775 None:intergenic 40.0%
CGTACAATACACCAAATGGT+GGG + chr3_3:64290796-64290815 Msa0464390:intron 40.0%
TTGAACAGGAGCTAAGAAAG+TGG + chr3_3:64290910-64290929 Msa0464390:intron 40.0%
GCAGCTTTCAACACTTTCTT+TGG - chr3_3:64290983-64291002 None:intergenic 40.0%
CAAAGAAGAAGGTTGAGCTT+TGG + chr3_3:64291011-64291030 Msa0464390:CDS 40.0%
GCCTTATGTTCCATACACTA+TGG + chr3_3:64291033-64291052 Msa0464390:CDS 40.0%
!! AGGAGGTGCTTTCTTATCAT+AGG - chr3_3:64291083-64291102 None:intergenic 40.0%
GATTGTTAGGGTGGTTGAAA+TGG + chr3_3:64291444-64291463 Msa0464390:three_prime_UTR 40.0%
TGGTTGAAATGGAACACACT+TGG + chr3_3:64291455-64291474 Msa0464390:three_prime_UTR 40.0%
CTTGAACAAACTAGAGTGGT+CGG + chr3_3:64290177-64290196 Msa0464390:five_prime_UTR 40.0%
! AAGCAGAAAAATGGGAGTAC+AGG + chr3_3:64290313-64290332 Msa0464390:exon 40.0%
! AGCAGAAAAATGGGAGTACA+GGG + chr3_3:64290314-64290333 Msa0464390:exon 40.0%
AAGGTTAAGCATGTCCTTTC+TGG + chr3_3:64290446-64290465 Msa0464390:CDS 40.0%
! CATGTCCTTTCTGGTGTTAA+AGG + chr3_3:64290455-64290474 Msa0464390:CDS 40.0%
ATTGTCGTCATGTGACTGAA+AGG + chr3_3:64290716-64290735 Msa0464390:intron 40.0%
TTACACAAGAGGCTGTTTAC+AGG - chr3_3:64290756-64290775 None:intergenic 40.0%
CGTACAATACACCAAATGGT+GGG + chr3_3:64290796-64290815 Msa0464390:intron 40.0%
TTGAACAGGAGCTAAGAAAG+TGG + chr3_3:64290910-64290929 Msa0464390:intron 40.0%
GCAGCTTTCAACACTTTCTT+TGG - chr3_3:64290983-64291002 None:intergenic 40.0%
CAAAGAAGAAGGTTGAGCTT+TGG + chr3_3:64291011-64291030 Msa0464390:CDS 40.0%
GCCTTATGTTCCATACACTA+TGG + chr3_3:64291033-64291052 Msa0464390:CDS 40.0%
!! AGGAGGTGCTTTCTTATCAT+AGG - chr3_3:64291083-64291102 None:intergenic 40.0%
GATTGTTAGGGTGGTTGAAA+TGG + chr3_3:64291444-64291463 Msa0464390:three_prime_UTR 40.0%
TGGTTGAAATGGAACACACT+TGG + chr3_3:64291455-64291474 Msa0464390:three_prime_UTR 40.0%
! CAATTTTACCCGTTGCGACA+AGG - chr3_3:64290700-64290719 None:intergenic 45.0%
GCTGCGTACAATACACCAAA+TGG + chr3_3:64290792-64290811 Msa0464390:intron 45.0%
GCGTACAATACACCAAATGG+TGG + chr3_3:64290795-64290814 Msa0464390:intron 45.0%
AGGAGCTAAGAAAGTGGATG+TGG + chr3_3:64290916-64290935 Msa0464390:intron 45.0%
CAGCAGAAAGTAACAGTGAG+TGG + chr3_3:64290947-64290966 Msa0464390:CDS 45.0%
! CAATTTTACCCGTTGCGACA+AGG - chr3_3:64290700-64290719 None:intergenic 45.0%
GCTGCGTACAATACACCAAA+TGG + chr3_3:64290792-64290811 Msa0464390:intron 45.0%
GCGTACAATACACCAAATGG+TGG + chr3_3:64290795-64290814 Msa0464390:intron 45.0%
AGGAGCTAAGAAAGTGGATG+TGG + chr3_3:64290916-64290935 Msa0464390:intron 45.0%
CAGCAGAAAGTAACAGTGAG+TGG + chr3_3:64290947-64290966 Msa0464390:CDS 45.0%
!! TATATATATATAATAAACAA+AGG + chr3_3:64290226-64290245 Msa0464390:five_prime_UTR 5.0%
!! TATATATATATAATAAACAA+AGG + chr3_3:64290226-64290245 Msa0464390:five_prime_UTR 5.0%
CATACGCAAGGTTCGAGAAG+GGG - chr3_3:64290823-64290842 None:intergenic 50.0%
GCATACGCAAGGTTCGAGAA+GGG - chr3_3:64290824-64290843 None:intergenic 50.0%
CTTCTCGAACCTTGCGTATG+CGG + chr3_3:64290823-64290842 Msa0464390:intron 50.0%
TTCTCGAACCTTGCGTATGC+GGG + chr3_3:64290824-64290843 Msa0464390:intron 50.0%
CATACGCAAGGTTCGAGAAG+GGG - chr3_3:64290823-64290842 None:intergenic 50.0%
GCATACGCAAGGTTCGAGAA+GGG - chr3_3:64290824-64290843 None:intergenic 50.0%
CTTCTCGAACCTTGCGTATG+CGG + chr3_3:64290823-64290842 Msa0464390:intron 50.0%
TTCTCGAACCTTGCGTATGC+GGG + chr3_3:64290824-64290843 Msa0464390:intron 50.0%
CTGTGAAGGTTGTGCTCGCA+AGG + chr3_3:64290427-64290446 Msa0464390:CDS 55.0%
GAGGGGTAACCTTGTCGCAA+CGG + chr3_3:64290688-64290707 Msa0464390:intron 55.0%
AGGGGTAACCTTGTCGCAAC+GGG + chr3_3:64290689-64290708 Msa0464390:intron 55.0%
CGCATACGCAAGGTTCGAGA+AGG - chr3_3:64290825-64290844 None:intergenic 55.0%
CTAAAGCTCCCGCATACGCA+AGG - chr3_3:64290835-64290854 None:intergenic 55.0%
! ATGCGGGAGCTTTAGTGCAC+TGG + chr3_3:64290840-64290859 Msa0464390:intron 55.0%
! TGCGGGAGCTTTAGTGCACT+GGG + chr3_3:64290841-64290860 Msa0464390:intron 55.0%
GGGTGAGCCACCATAGTGTA+TGG - chr3_3:64291046-64291065 None:intergenic 55.0%
CTGTGAAGGTTGTGCTCGCA+AGG + chr3_3:64290427-64290446 Msa0464390:CDS 55.0%
GAGGGGTAACCTTGTCGCAA+CGG + chr3_3:64290688-64290707 Msa0464390:intron 55.0%
AGGGGTAACCTTGTCGCAAC+GGG + chr3_3:64290689-64290708 Msa0464390:intron 55.0%
CGCATACGCAAGGTTCGAGA+AGG - chr3_3:64290825-64290844 None:intergenic 55.0%
CTAAAGCTCCCGCATACGCA+AGG - chr3_3:64290835-64290854 None:intergenic 55.0%
! ATGCGGGAGCTTTAGTGCAC+TGG + chr3_3:64290840-64290859 Msa0464390:intron 55.0%
! TGCGGGAGCTTTAGTGCACT+GGG + chr3_3:64290841-64290860 Msa0464390:intron 55.0%
GGGTGAGCCACCATAGTGTA+TGG - chr3_3:64291046-64291065 None:intergenic 55.0%
CGAGAAGGGGTCCCACCATT+TGG - chr3_3:64290810-64290829 None:intergenic 60.0%
CGAGAAGGGGTCCCACCATT+TGG - chr3_3:64290810-64290829 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 64290092 64291478 64290092 ID=Msa0464390;Name=Msa0464390
chr3_3 mRNA 64290092 64291478 64290092 ID=Msa0464390-mRNA-1;Parent=Msa0464390;Name=Msa0464390-mRNA-1;_AED=0.00;_eAED=0.00;_QI=231|1|1|1|1|1|2|265|149
chr3_3 exon 64290092 64290476 64290092 ID=Msa0464390-mRNA-1:exon:14107;Parent=Msa0464390-mRNA-1
chr3_3 exon 64290918 64291478 64290918 ID=Msa0464390-mRNA-1:exon:14108;Parent=Msa0464390-mRNA-1
chr3_3 five_prime_UTR 64290092 64290322 64290092 ID=Msa0464390-mRNA-1:five_prime_utr;Parent=Msa0464390-mRNA-1
chr3_3 CDS 64290323 64290476 64290323 ID=Msa0464390-mRNA-1:cds;Parent=Msa0464390-mRNA-1
chr3_3 CDS 64290918 64291213 64290918 ID=Msa0464390-mRNA-1:cds;Parent=Msa0464390-mRNA-1
chr3_3 three_prime_UTR 64291214 64291478 64291214 ID=Msa0464390-mRNA-1:three_prime_utr;Parent=Msa0464390-mRNA-1
Gene Sequence

>Msa0464390

ATGGGAGTACAGGGTACCCTAGAATACTTGTCTGATTTACTCAGTAGCACAAAAAAGAAAAAGAAGAAGCAAACTCAAACTGTATCTCTTAAAATCAGAATGGACTGTGAAGGTTGTGCTCGCAAGGTTAAGCATGTCCTTTCTGGTGTTAAAGGAGCTAAGAAAGTGGATGTGGACTTGAAGCAGCAGAAAGTAACAGTGAGTGGATATGTTGAACCAAAGAAAGTGTTGAAAGCTGCTCAATCAACAAAGAAGAAGGTTGAGCTTTGGCCTTATGTTCCATACACTATGGTGGCTCACCCTTATATTTCACAAGCCTATGATAAGAAAGCACCTCCTAATATGGTTAGAAAGGTTGCTGACACTTCCAACATAAAAGAGTCCACTTTTGATGATAGCTATGTTGAAATGTTCAGTGATGAAAATCCAAATGCTTGTTCTATTATGTAG
Protein sequence

>Msa0464390

MGVQGTLEYLSDLLSSTKKKKKKQTQTVSLKIRMDCEGCARKVKHVLSGVKGAKKVDVDLKQQKVTVSGYVEPKKVLKAAQSTKKKVELWPYVPYTMVAHPYISQAYDKKAPPNMVRKVADTSNIKESTFDDSYVEMFSDENPNACSIM*