AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1398370

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1398370 MtrunA17_Chr3g0115421 99.329 149 1 0 1 149 1 149 7.87e-108 303
Msa1398370 MtrunA17_Chr8g0382721 62.745 153 53 2 1 149 1 153 2.58e-59 181
Msa1398370 MtrunA17_Chr3g0124411 60.390 154 54 3 1 149 1 152 1.72e-58 178
Msa1398370 MtrunA17_Chr4g0036931 51.613 155 65 3 5 149 2 156 2.91e-49 155
Msa1398370 MtrunA17_Chr1g0160201 51.020 147 69 2 5 149 2 147 7.50e-48 151
Msa1398370 MtrunA17_Chr3g0145621 47.436 156 75 2 1 149 1 156 9.58e-48 151
Msa1398370 MtrunA17_Chr5g0405811 48.077 156 70 3 5 149 2 157 3.29e-45 145
Msa1398370 MtrunA17_Chr5g0407821 46.897 145 76 1 5 149 2 145 1.59e-44 143
Msa1398370 MtrunA17_Chr6g0448861 44.805 154 76 3 5 149 2 155 4.73e-43 139
Msa1398370 MtrunA17_Chr7g0251491 43.537 147 79 3 5 149 26 170 6.88e-38 127
Msa1398370 MtrunA17_Chr5g0422891 38.095 147 88 2 5 149 2 147 1.25e-32 112
Msa1398370 MtrunA17_Chr2g0325601 33.333 138 73 3 30 149 6 142 8.99e-20 79.7
Msa1398370 MtrunA17_Chr1g0189221 48.148 81 38 1 26 102 53 133 2.76e-19 79.7
Msa1398370 MtrunA17_Chr6g0471231 38.168 131 66 4 33 149 11 140 7.58e-19 77.4
Msa1398370 MtrunA17_Chr1g0198341 50.000 66 33 0 25 90 3 68 6.72e-16 68.2
Msa1398370 MtrunA17_Chr7g0218981 43.939 66 37 0 25 90 3 68 3.36e-15 67.8
Msa1398370 MtrunA17_Chr6g0460361 37.963 108 56 4 52 149 4 110 5.44e-14 63.9
Msa1398370 MtrunA17_Chr7g0272571 43.077 65 37 0 27 91 34 98 6.72e-13 64.7
Msa1398370 MtrunA17_Chr1g0192131 39.744 78 46 1 15 91 4 81 9.60e-13 64.3
Msa1398370 MtrunA17_Chr7g0269081 40.000 65 39 0 26 90 22 86 1.29e-12 63.5
Msa1398370 MtrunA17_Chr1g0191671 44.615 65 36 0 26 90 10 74 2.00e-12 63.5
Msa1398370 MtrunA17_Chr1g0208301 43.077 65 37 0 26 90 13 77 2.41e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1398370 AT4G08570.1 76.000 150 35 1 1 149 1 150 4.39e-81 236
Msa1398370 AT4G39700.1 60.127 158 54 3 1 149 1 158 3.51e-57 176
Msa1398370 AT1G22990.1 55.921 152 59 3 5 149 2 152 6.52e-57 175
Msa1398370 AT1G22990.2 53.642 151 59 4 5 149 2 147 5.63e-53 164
Msa1398370 AT1G71050.1 51.974 152 70 3 1 149 1 152 2.51e-48 153
Msa1398370 AT4G38580.1 48.684 152 71 3 5 149 2 153 1.50e-45 146
Msa1398370 AT4G38580.2 48.684 152 71 3 5 149 2 153 1.50e-45 146
Msa1398370 AT5G66110.3 50.345 145 67 3 10 149 3 147 7.51e-45 144
Msa1398370 AT5G66110.1 50.345 145 67 3 10 149 3 147 7.51e-45 144
Msa1398370 AT5G66110.2 53.719 121 51 3 34 149 1 121 6.26e-40 130
Msa1398370 AT4G35060.1 41.447 152 82 3 5 149 2 153 3.72e-38 127
Msa1398370 AT5G17450.1 44.737 152 73 4 5 149 2 149 1.34e-36 123
Msa1398370 AT5G17450.2 46.078 102 47 3 52 149 8 105 2.60e-24 90.9
Msa1398370 AT2G18196.1 37.594 133 74 3 26 149 46 178 1.02e-23 91.3
Msa1398370 AT1G06330.1 35.135 148 71 3 27 149 3 150 2.65e-22 87.0
Msa1398370 AT2G18196.2 36.000 125 71 3 34 149 1 125 6.82e-20 80.1
Msa1398370 AT4G10465.1 35.000 140 82 3 19 149 44 183 4.10e-19 79.7
Msa1398370 AT3G56891.1 31.333 150 74 4 28 149 18 166 2.24e-18 77.4
Msa1398370 AT1G29100.2 34.014 147 74 4 25 149 2 147 6.94e-18 75.5
Msa1398370 AT3G56891.2 31.250 144 70 4 34 149 1 143 2.10e-17 74.3
Msa1398370 AT1G29100.1 33.803 142 71 4 30 149 1 141 8.03e-17 72.4
Msa1398370 AT1G66240.1 47.826 69 36 0 22 90 29 97 5.08e-16 69.7
Msa1398370 AT3G56240.3 46.970 66 35 0 26 91 3 68 2.86e-15 68.2
Msa1398370 AT3G56240.1 46.970 66 35 0 26 91 3 68 2.86e-15 68.2
Msa1398370 AT5G27690.1 45.161 62 34 0 30 91 34 95 7.73e-14 67.8
Msa1398370 AT1G56210.1 43.478 69 39 0 26 94 40 108 1.71e-13 66.6
Msa1398370 AT3G48970.1 36.879 141 71 6 25 149 2 140 3.69e-13 63.2
Msa1398370 AT3G48970.2 37.410 139 69 6 27 149 2 138 5.12e-13 62.8
Msa1398370 AT3G48970.3 37.410 139 69 6 27 149 2 138 5.12e-13 62.8
Msa1398370 AT1G66240.2 49.123 57 29 0 34 90 1 57 2.30e-12 58.9
Msa1398370 AT3G56240.2 44.828 58 32 0 34 91 1 58 4.61e-12 59.3

Find 47 sgRNAs with CRISPR-Local

Find 176 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATTAATTTCTCACCCCTTA+TGG 0.244698 tig0022248:+92665 Msa1398370:three_prime_UTR
AAGGTTAAGCATGTCCTTTC+TGG 0.256831 tig0022248:+91880 Msa1398370:CDS
GCAACCTTTCTAACCATATT+AGG 0.261201 tig0022248:-92534 None:intergenic
CATGTCCTTTCTGGTGTTAA+AGG 0.262150 tig0022248:+91889 Msa1398370:CDS
TCATAGGCTTGTGAAATATA+AGG 0.273413 tig0022248:-92498 None:intergenic
GATAATGATGATTGATTGTT+AGG 0.273640 tig0022248:+92865 Msa1398370:three_prime_UTR
GCAGCTTTCAACACTTTCTT+TGG 0.279745 tig0022248:-92414 None:intergenic
GTAAATCAGACAAGTATTCT+AGG 0.300083 tig0022248:-91774 None:intergenic
TGAGGAGAAAATAATGTAAT+AGG 0.320083 tig0022248:+92699 Msa1398370:three_prime_UTR
CAAGTATGAGAACAAAATTA+TGG 0.356872 tig0022248:-91592 None:intergenic
TAAGAAAGCACCTCCTAATA+TGG 0.356938 tig0022248:+92521 Msa1398370:CDS
GCCTTATGTTCCATACACTA+TGG 0.372587 tig0022248:+92467 Msa1398370:CDS
AGGAGGTGCTTTCTTATCAT+AGG 0.375360 tig0022248:-92514 None:intergenic
TACATAATAGAACAAGCATT+TGG 0.390614 tig0022248:-92624 None:intergenic
GAGATAGAGATATGTGATAG+AGG 0.407764 tig0022248:+92741 Msa1398370:three_prime_UTR
GATTGTTAGGGTGGTTGAAA+TGG 0.420007 tig0022248:+92878 Msa1398370:three_prime_UTR
TCAAAGTGTGATTACCATAA+GGG 0.421588 tig0022248:-92679 None:intergenic
ATTCAAGAAATAAACTACTT+TGG 0.423174 tig0022248:-91683 None:intergenic
CTCAAAGTGTGATTACCATA+AGG 0.439579 tig0022248:-92680 None:intergenic
CATAGGCTTGTGAAATATAA+GGG 0.443566 tig0022248:-92497 None:intergenic
CAAAGAAGAAGGTTGAGCTT+TGG 0.454326 tig0022248:+92445 Msa1398370:CDS
ACCTCCTAATATGGTTAGAA+AGG 0.456268 tig0022248:+92530 Msa1398370:CDS
AATTGACTGATTGAAGTGTA+TGG 0.461832 tig0022248:-91553 None:intergenic
ACCATAGTGTATGGAACATA+AGG 0.464116 tig0022248:-92468 None:intergenic
AACATAGCTATCATCAAAAG+TGG 0.479724 tig0022248:-92580 None:intergenic
GGGTGAGCCACCATAGTGTA+TGG 0.494549 tig0022248:-92477 None:intergenic
CTTATGGTAATCACACTTTG+AGG 0.514753 tig0022248:+92681 Msa1398370:three_prime_UTR
TGTATCTCTTAAAATCAGAA+TGG 0.514900 tig0022248:+91837 Msa1398370:CDS
TGCTCAATCAACAAAGAAGA+AGG 0.535682 tig0022248:+92434 Msa1398370:CDS
ATAATGATGATTGATTGTTA+GGG 0.541805 tig0022248:+92866 Msa1398370:three_prime_UTR
AGGAGCTAAGAAAGTGGATG+TGG 0.542853 tig0022248:+92350 Msa1398370:intron
AAGTGTATGGAAATATAGAA+AGG 0.553419 tig0022248:-91540 None:intergenic
TAAATCAGACAAGTATTCTA+GGG 0.567273 tig0022248:-91773 None:intergenic
CTGTGAAGGTTGTGCTCGCA+AGG 0.570301 tig0022248:+91861 Msa1398370:CDS
ATGATGATTGATTGTTAGGG+TGG 0.571522 tig0022248:+92869 Msa1398370:three_prime_UTR
ACCTTTCTAACCATATTAGG+AGG 0.574085 tig0022248:-92531 None:intergenic
CAGCAGAAAGTAACAGTGAG+TGG 0.576096 tig0022248:+92381 Msa1398370:CDS
TTGAACAGGAGCTAAGAAAG+TGG 0.579198 tig0022248:+92344 Msa1398370:intron
ATAGAGATATGTGATAGAGG+AGG 0.586370 tig0022248:+92744 Msa1398370:three_prime_UTR
CAAAGTGTGATTACCATAAG+GGG 0.600902 tig0022248:-92678 None:intergenic
CTTGAACAAACTAGAGTGGT+CGG 0.607671 tig0022248:+91611 Msa1398370:five_prime_UTR
AAAATCAGAATGGACTGTGA+AGG 0.610949 tig0022248:+91847 Msa1398370:CDS
TTATGTTCCATACACTATGG+TGG 0.612160 tig0022248:+92470 Msa1398370:CDS
AAGTACCTTTAACACCAGAA+AGG 0.613905 tig0022248:-91894 None:intergenic
CATACTTGAACAAACTAGAG+TGG 0.652576 tig0022248:+91607 Msa1398370:five_prime_UTR
TGGTTGAAATGGAACACACT+TGG 0.653186 tig0022248:+92889 Msa1398370:three_prime_UTR
AGTAAGCTAGAATCATTGTG+AGG 0.719657 tig0022248:+91712 Msa1398370:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATTATATATATATAGACA+TGG - tig0022248:91656-91675 None:intergenic 10.0%
!!! AATAATAATATAATTTTGCA+TGG + tig0022248:92822-92841 Msa1398370:three_prime_UTR 10.0%
!! TTATTATATATATATAGACA+TGG - tig0022248:91656-91675 None:intergenic 10.0%
!!! AATAATAATATAATTTTGCA+TGG + tig0022248:92822-92841 Msa1398370:three_prime_UTR 10.0%
!!! TAATGCATAATTTTAAATTG+TGG - tig0022248:92085-92104 None:intergenic 15.0%
!!! ATTGTTTTTAGTTCATTTAT+TGG + tig0022248:92306-92325 Msa1398370:intron 15.0%
!!! AATTAATTTGAAATTTGAAC+AGG + tig0022248:92330-92349 Msa1398370:intron 15.0%
!!! TAATGCATAATTTTAAATTG+TGG - tig0022248:92085-92104 None:intergenic 15.0%
!!! ATTGTTTTTAGTTCATTTAT+TGG + tig0022248:92306-92325 Msa1398370:intron 15.0%
!!! AATTAATTTGAAATTTGAAC+AGG + tig0022248:92330-92349 Msa1398370:intron 15.0%
!! ATTCAAGAAATAAACTACTT+TGG - tig0022248:91686-91705 None:intergenic 20.0%
!!! TATTGTTTTTAGTTCATTTG+AGG + tig0022248:92103-92122 Msa1398370:intron 20.0%
!!! ATTGTTTTTAGTTCATTTGA+GGG + tig0022248:92104-92123 Msa1398370:intron 20.0%
!! ATAATGATGATTGATTGTTA+GGG + tig0022248:92866-92885 Msa1398370:three_prime_UTR 20.0%
!! ATTCAAGAAATAAACTACTT+TGG - tig0022248:91686-91705 None:intergenic 20.0%
!!! TATTGTTTTTAGTTCATTTG+AGG + tig0022248:92103-92122 Msa1398370:intron 20.0%
!!! ATTGTTTTTAGTTCATTTGA+GGG + tig0022248:92104-92123 Msa1398370:intron 20.0%
!! ATAATGATGATTGATTGTTA+GGG + tig0022248:92866-92885 Msa1398370:three_prime_UTR 20.0%
! AAGTGTATGGAAATATAGAA+AGG - tig0022248:91543-91562 None:intergenic 25.0%
! CAAGTATGAGAACAAAATTA+TGG - tig0022248:91595-91614 None:intergenic 25.0%
! TAAATCAGACAAGTATTCTA+GGG - tig0022248:91776-91795 None:intergenic 25.0%
! TGTATCTCTTAAAATCAGAA+TGG + tig0022248:91837-91856 Msa1398370:CDS 25.0%
!!! TTGTTTTTAGTTCATTTGAG+GGG + tig0022248:92105-92124 Msa1398370:intron 25.0%
! CCTCTTGTGTAAAAAAAAAT+GGG + tig0022248:92198-92217 Msa1398370:intron 25.0%
! CTCTTGTGTAAAAAAAAATG+GGG + tig0022248:92199-92218 Msa1398370:intron 25.0%
! TACATAATAGAACAAGCATT+TGG - tig0022248:92627-92646 None:intergenic 25.0%
! TGAGGAGAAAATAATGTAAT+AGG + tig0022248:92699-92718 Msa1398370:three_prime_UTR 25.0%
! GATAATGATGATTGATTGTT+AGG + tig0022248:92865-92884 Msa1398370:three_prime_UTR 25.0%
! AAGTGTATGGAAATATAGAA+AGG - tig0022248:91543-91562 None:intergenic 25.0%
! CAAGTATGAGAACAAAATTA+TGG - tig0022248:91595-91614 None:intergenic 25.0%
! TAAATCAGACAAGTATTCTA+GGG - tig0022248:91776-91795 None:intergenic 25.0%
! TGTATCTCTTAAAATCAGAA+TGG + tig0022248:91837-91856 Msa1398370:CDS 25.0%
!!! TTGTTTTTAGTTCATTTGAG+GGG + tig0022248:92105-92124 Msa1398370:intron 25.0%
! CCTCTTGTGTAAAAAAAAAT+GGG + tig0022248:92198-92217 Msa1398370:intron 25.0%
! CTCTTGTGTAAAAAAAAATG+GGG + tig0022248:92199-92218 Msa1398370:intron 25.0%
! TACATAATAGAACAAGCATT+TGG - tig0022248:92627-92646 None:intergenic 25.0%
! TGAGGAGAAAATAATGTAAT+AGG + tig0022248:92699-92718 Msa1398370:three_prime_UTR 25.0%
! GATAATGATGATTGATTGTT+AGG + tig0022248:92865-92884 Msa1398370:three_prime_UTR 25.0%
AATTGACTGATTGAAGTGTA+TGG - tig0022248:91556-91575 None:intergenic 30.0%
AGCAAGAATAAGCAGAAAAA+TGG + tig0022248:91738-91757 Msa1398370:exon 30.0%
GCAAGAATAAGCAGAAAAAT+GGG + tig0022248:91739-91758 Msa1398370:exon 30.0%
GTAAATCAGACAAGTATTCT+AGG - tig0022248:91777-91796 None:intergenic 30.0%
! AAACGAGGCAATGATTTTTA+AGG - tig0022248:91950-91969 None:intergenic 30.0%
!! CATTGCCTCGTTTTAAATTA+CGG + tig0022248:91957-91976 Msa1398370:intron 30.0%
! ACTTTTATATTGCGCAATTG+TGG + tig0022248:92052-92071 Msa1398370:intron 30.0%
GCCTCTTGTGTAAAAAAAAA+TGG + tig0022248:92197-92216 Msa1398370:intron 30.0%
!!! CCCATTTTTTTTTACACAAG+AGG - tig0022248:92201-92220 None:intergenic 30.0%
GTGTAAAAAAAAATGGGGTA+AGG + tig0022248:92204-92223 Msa1398370:intron 30.0%
CATAGGCTTGTGAAATATAA+GGG - tig0022248:92500-92519 None:intergenic 30.0%
TCATAGGCTTGTGAAATATA+AGG - tig0022248:92501-92520 None:intergenic 30.0%
!! AAAAGTGGACTCTTTTATGT+TGG - tig0022248:92568-92587 None:intergenic 30.0%
AACATAGCTATCATCAAAAG+TGG - tig0022248:92583-92602 None:intergenic 30.0%
TCAAAGTGTGATTACCATAA+GGG - tig0022248:92682-92701 None:intergenic 30.0%
AATTGACTGATTGAAGTGTA+TGG - tig0022248:91556-91575 None:intergenic 30.0%
AGCAAGAATAAGCAGAAAAA+TGG + tig0022248:91738-91757 Msa1398370:exon 30.0%
GCAAGAATAAGCAGAAAAAT+GGG + tig0022248:91739-91758 Msa1398370:exon 30.0%
GTAAATCAGACAAGTATTCT+AGG - tig0022248:91777-91796 None:intergenic 30.0%
! AAACGAGGCAATGATTTTTA+AGG - tig0022248:91950-91969 None:intergenic 30.0%
!! CATTGCCTCGTTTTAAATTA+CGG + tig0022248:91957-91976 Msa1398370:intron 30.0%
! ACTTTTATATTGCGCAATTG+TGG + tig0022248:92052-92071 Msa1398370:intron 30.0%
GCCTCTTGTGTAAAAAAAAA+TGG + tig0022248:92197-92216 Msa1398370:intron 30.0%
!!! CCCATTTTTTTTTACACAAG+AGG - tig0022248:92201-92220 None:intergenic 30.0%
GTGTAAAAAAAAATGGGGTA+AGG + tig0022248:92204-92223 Msa1398370:intron 30.0%
CATAGGCTTGTGAAATATAA+GGG - tig0022248:92500-92519 None:intergenic 30.0%
TCATAGGCTTGTGAAATATA+AGG - tig0022248:92501-92520 None:intergenic 30.0%
!! AAAAGTGGACTCTTTTATGT+TGG - tig0022248:92568-92587 None:intergenic 30.0%
AACATAGCTATCATCAAAAG+TGG - tig0022248:92583-92602 None:intergenic 30.0%
TCAAAGTGTGATTACCATAA+GGG - tig0022248:92682-92701 None:intergenic 30.0%
CATACTTGAACAAACTAGAG+TGG + tig0022248:91607-91626 Msa1398370:five_prime_UTR 35.0%
AGTAAGCTAGAATCATTGTG+AGG + tig0022248:91712-91731 Msa1398370:five_prime_UTR 35.0%
!! AAAATCAGAATGGACTGTGA+AGG + tig0022248:91847-91866 Msa1398370:CDS 35.0%
AAGTACCTTTAACACCAGAA+AGG - tig0022248:91897-91916 None:intergenic 35.0%
! TGCAACCGTAATTTAAAACG+AGG - tig0022248:91965-91984 None:intergenic 35.0%
!!! CTACAGCAGTCGATATTTTT+TGG - tig0022248:92021-92040 None:intergenic 35.0%
TGCTCAATCAACAAAGAAGA+AGG + tig0022248:92434-92453 Msa1398370:CDS 35.0%
ACCATAGTGTATGGAACATA+AGG - tig0022248:92471-92490 None:intergenic 35.0%
TTATGTTCCATACACTATGG+TGG + tig0022248:92470-92489 Msa1398370:CDS 35.0%
!! TAAGAAAGCACCTCCTAATA+TGG + tig0022248:92521-92540 Msa1398370:CDS 35.0%
ACCTCCTAATATGGTTAGAA+AGG + tig0022248:92530-92549 Msa1398370:CDS 35.0%
ACCTTTCTAACCATATTAGG+AGG - tig0022248:92534-92553 None:intergenic 35.0%
GCAACCTTTCTAACCATATT+AGG - tig0022248:92537-92556 None:intergenic 35.0%
GATTAATTTCTCACCCCTTA+TGG + tig0022248:92665-92684 Msa1398370:three_prime_UTR 35.0%
CAAAGTGTGATTACCATAAG+GGG - tig0022248:92681-92700 None:intergenic 35.0%
CTCAAAGTGTGATTACCATA+AGG - tig0022248:92683-92702 None:intergenic 35.0%
CTTATGGTAATCACACTTTG+AGG + tig0022248:92681-92700 Msa1398370:three_prime_UTR 35.0%
GAGATAGAGATATGTGATAG+AGG + tig0022248:92741-92760 Msa1398370:three_prime_UTR 35.0%
ATAGAGATATGTGATAGAGG+AGG + tig0022248:92744-92763 Msa1398370:three_prime_UTR 35.0%
ATGATGATTGATTGTTAGGG+TGG + tig0022248:92869-92888 Msa1398370:three_prime_UTR 35.0%
CATACTTGAACAAACTAGAG+TGG + tig0022248:91607-91626 Msa1398370:five_prime_UTR 35.0%
AGTAAGCTAGAATCATTGTG+AGG + tig0022248:91712-91731 Msa1398370:five_prime_UTR 35.0%
!! AAAATCAGAATGGACTGTGA+AGG + tig0022248:91847-91866 Msa1398370:CDS 35.0%
AAGTACCTTTAACACCAGAA+AGG - tig0022248:91897-91916 None:intergenic 35.0%
! TGCAACCGTAATTTAAAACG+AGG - tig0022248:91965-91984 None:intergenic 35.0%
!!! CTACAGCAGTCGATATTTTT+TGG - tig0022248:92021-92040 None:intergenic 35.0%
TGCTCAATCAACAAAGAAGA+AGG + tig0022248:92434-92453 Msa1398370:CDS 35.0%
ACCATAGTGTATGGAACATA+AGG - tig0022248:92471-92490 None:intergenic 35.0%
TTATGTTCCATACACTATGG+TGG + tig0022248:92470-92489 Msa1398370:CDS 35.0%
!! TAAGAAAGCACCTCCTAATA+TGG + tig0022248:92521-92540 Msa1398370:CDS 35.0%
ACCTCCTAATATGGTTAGAA+AGG + tig0022248:92530-92549 Msa1398370:CDS 35.0%
ACCTTTCTAACCATATTAGG+AGG - tig0022248:92534-92553 None:intergenic 35.0%
GCAACCTTTCTAACCATATT+AGG - tig0022248:92537-92556 None:intergenic 35.0%
GATTAATTTCTCACCCCTTA+TGG + tig0022248:92665-92684 Msa1398370:three_prime_UTR 35.0%
CAAAGTGTGATTACCATAAG+GGG - tig0022248:92681-92700 None:intergenic 35.0%
CTCAAAGTGTGATTACCATA+AGG - tig0022248:92683-92702 None:intergenic 35.0%
CTTATGGTAATCACACTTTG+AGG + tig0022248:92681-92700 Msa1398370:three_prime_UTR 35.0%
GAGATAGAGATATGTGATAG+AGG + tig0022248:92741-92760 Msa1398370:three_prime_UTR 35.0%
ATAGAGATATGTGATAGAGG+AGG + tig0022248:92744-92763 Msa1398370:three_prime_UTR 35.0%
ATGATGATTGATTGTTAGGG+TGG + tig0022248:92869-92888 Msa1398370:three_prime_UTR 35.0%
CTTGAACAAACTAGAGTGGT+CGG + tig0022248:91611-91630 Msa1398370:five_prime_UTR 40.0%
! AAGCAGAAAAATGGGAGTAC+AGG + tig0022248:91747-91766 Msa1398370:exon 40.0%
! AGCAGAAAAATGGGAGTACA+GGG + tig0022248:91748-91767 Msa1398370:exon 40.0%
AAGGTTAAGCATGTCCTTTC+TGG + tig0022248:91880-91899 Msa1398370:CDS 40.0%
! CATGTCCTTTCTGGTGTTAA+AGG + tig0022248:91889-91908 Msa1398370:CDS 40.0%
ATTGTCGTCATGTGACTGAA+AGG + tig0022248:92150-92169 Msa1398370:intron 40.0%
TTACACAAGAGGCTGTTTAC+AGG - tig0022248:92190-92209 None:intergenic 40.0%
CGTACAATACACCAAATGGT+GGG + tig0022248:92230-92249 Msa1398370:intron 40.0%
TTGAACAGGAGCTAAGAAAG+TGG + tig0022248:92344-92363 Msa1398370:intron 40.0%
GCAGCTTTCAACACTTTCTT+TGG - tig0022248:92417-92436 None:intergenic 40.0%
CAAAGAAGAAGGTTGAGCTT+TGG + tig0022248:92445-92464 Msa1398370:CDS 40.0%
GCCTTATGTTCCATACACTA+TGG + tig0022248:92467-92486 Msa1398370:CDS 40.0%
!! AGGAGGTGCTTTCTTATCAT+AGG - tig0022248:92517-92536 None:intergenic 40.0%
GATTGTTAGGGTGGTTGAAA+TGG + tig0022248:92878-92897 Msa1398370:three_prime_UTR 40.0%
TGGTTGAAATGGAACACACT+TGG + tig0022248:92889-92908 Msa1398370:three_prime_UTR 40.0%
CTTGAACAAACTAGAGTGGT+CGG + tig0022248:91611-91630 Msa1398370:five_prime_UTR 40.0%
! AAGCAGAAAAATGGGAGTAC+AGG + tig0022248:91747-91766 Msa1398370:exon 40.0%
! AGCAGAAAAATGGGAGTACA+GGG + tig0022248:91748-91767 Msa1398370:exon 40.0%
AAGGTTAAGCATGTCCTTTC+TGG + tig0022248:91880-91899 Msa1398370:CDS 40.0%
! CATGTCCTTTCTGGTGTTAA+AGG + tig0022248:91889-91908 Msa1398370:CDS 40.0%
ATTGTCGTCATGTGACTGAA+AGG + tig0022248:92150-92169 Msa1398370:intron 40.0%
TTACACAAGAGGCTGTTTAC+AGG - tig0022248:92190-92209 None:intergenic 40.0%
CGTACAATACACCAAATGGT+GGG + tig0022248:92230-92249 Msa1398370:intron 40.0%
TTGAACAGGAGCTAAGAAAG+TGG + tig0022248:92344-92363 Msa1398370:intron 40.0%
GCAGCTTTCAACACTTTCTT+TGG - tig0022248:92417-92436 None:intergenic 40.0%
CAAAGAAGAAGGTTGAGCTT+TGG + tig0022248:92445-92464 Msa1398370:CDS 40.0%
GCCTTATGTTCCATACACTA+TGG + tig0022248:92467-92486 Msa1398370:CDS 40.0%
!! AGGAGGTGCTTTCTTATCAT+AGG - tig0022248:92517-92536 None:intergenic 40.0%
GATTGTTAGGGTGGTTGAAA+TGG + tig0022248:92878-92897 Msa1398370:three_prime_UTR 40.0%
TGGTTGAAATGGAACACACT+TGG + tig0022248:92889-92908 Msa1398370:three_prime_UTR 40.0%
! CAATTTTACCCGTTGCGACA+AGG - tig0022248:92134-92153 None:intergenic 45.0%
GCTGCGTACAATACACCAAA+TGG + tig0022248:92226-92245 Msa1398370:intron 45.0%
GCGTACAATACACCAAATGG+TGG + tig0022248:92229-92248 Msa1398370:intron 45.0%
AGGAGCTAAGAAAGTGGATG+TGG + tig0022248:92350-92369 Msa1398370:intron 45.0%
CAGCAGAAAGTAACAGTGAG+TGG + tig0022248:92381-92400 Msa1398370:CDS 45.0%
! CAATTTTACCCGTTGCGACA+AGG - tig0022248:92134-92153 None:intergenic 45.0%
GCTGCGTACAATACACCAAA+TGG + tig0022248:92226-92245 Msa1398370:intron 45.0%
GCGTACAATACACCAAATGG+TGG + tig0022248:92229-92248 Msa1398370:intron 45.0%
AGGAGCTAAGAAAGTGGATG+TGG + tig0022248:92350-92369 Msa1398370:intron 45.0%
CAGCAGAAAGTAACAGTGAG+TGG + tig0022248:92381-92400 Msa1398370:CDS 45.0%
!! TATATATATATAATAAACAA+AGG + tig0022248:91660-91679 Msa1398370:five_prime_UTR 5.0%
!! TATATATATATAATAAACAA+AGG + tig0022248:91660-91679 Msa1398370:five_prime_UTR 5.0%
CATACGCAAGGTTCGAGAAG+GGG - tig0022248:92257-92276 None:intergenic 50.0%
GCATACGCAAGGTTCGAGAA+GGG - tig0022248:92258-92277 None:intergenic 50.0%
CTTCTCGAACCTTGCGTATG+CGG + tig0022248:92257-92276 Msa1398370:intron 50.0%
TTCTCGAACCTTGCGTATGC+GGG + tig0022248:92258-92277 Msa1398370:intron 50.0%
CATACGCAAGGTTCGAGAAG+GGG - tig0022248:92257-92276 None:intergenic 50.0%
GCATACGCAAGGTTCGAGAA+GGG - tig0022248:92258-92277 None:intergenic 50.0%
CTTCTCGAACCTTGCGTATG+CGG + tig0022248:92257-92276 Msa1398370:intron 50.0%
TTCTCGAACCTTGCGTATGC+GGG + tig0022248:92258-92277 Msa1398370:intron 50.0%
CTGTGAAGGTTGTGCTCGCA+AGG + tig0022248:91861-91880 Msa1398370:CDS 55.0%
GAGGGGTAACCTTGTCGCAA+CGG + tig0022248:92122-92141 Msa1398370:intron 55.0%
AGGGGTAACCTTGTCGCAAC+GGG + tig0022248:92123-92142 Msa1398370:intron 55.0%
CGCATACGCAAGGTTCGAGA+AGG - tig0022248:92259-92278 None:intergenic 55.0%
CTAAAGCTCCCGCATACGCA+AGG - tig0022248:92269-92288 None:intergenic 55.0%
! ATGCGGGAGCTTTAGTGCAC+TGG + tig0022248:92274-92293 Msa1398370:intron 55.0%
! TGCGGGAGCTTTAGTGCACT+GGG + tig0022248:92275-92294 Msa1398370:intron 55.0%
GGGTGAGCCACCATAGTGTA+TGG - tig0022248:92480-92499 None:intergenic 55.0%
CTGTGAAGGTTGTGCTCGCA+AGG + tig0022248:91861-91880 Msa1398370:CDS 55.0%
GAGGGGTAACCTTGTCGCAA+CGG + tig0022248:92122-92141 Msa1398370:intron 55.0%
AGGGGTAACCTTGTCGCAAC+GGG + tig0022248:92123-92142 Msa1398370:intron 55.0%
CGCATACGCAAGGTTCGAGA+AGG - tig0022248:92259-92278 None:intergenic 55.0%
CTAAAGCTCCCGCATACGCA+AGG - tig0022248:92269-92288 None:intergenic 55.0%
! ATGCGGGAGCTTTAGTGCAC+TGG + tig0022248:92274-92293 Msa1398370:intron 55.0%
! TGCGGGAGCTTTAGTGCACT+GGG + tig0022248:92275-92294 Msa1398370:intron 55.0%
GGGTGAGCCACCATAGTGTA+TGG - tig0022248:92480-92499 None:intergenic 55.0%
CGAGAAGGGGTCCCACCATT+TGG - tig0022248:92244-92263 None:intergenic 60.0%
CGAGAAGGGGTCCCACCATT+TGG - tig0022248:92244-92263 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
tig0022248 gene 91526 92912 91526 ID=Msa1398370;Name=Msa1398370
tig0022248 mRNA 91526 92912 91526 ID=Msa1398370-mRNA-1;Parent=Msa1398370;Name=Msa1398370-mRNA-1;_AED=0.00;_eAED=0.00;_QI=231|1|1|1|1|1|2|265|149
tig0022248 exon 91526 91910 91526 ID=Msa1398370-mRNA-1:exon:16538;Parent=Msa1398370-mRNA-1
tig0022248 exon 92352 92912 92352 ID=Msa1398370-mRNA-1:exon:16539;Parent=Msa1398370-mRNA-1
tig0022248 five_prime_UTR 91526 91756 91526 ID=Msa1398370-mRNA-1:five_prime_utr;Parent=Msa1398370-mRNA-1
tig0022248 CDS 91757 91910 91757 ID=Msa1398370-mRNA-1:cds;Parent=Msa1398370-mRNA-1
tig0022248 CDS 92352 92647 92352 ID=Msa1398370-mRNA-1:cds;Parent=Msa1398370-mRNA-1
tig0022248 three_prime_UTR 92648 92912 92648 ID=Msa1398370-mRNA-1:three_prime_utr;Parent=Msa1398370-mRNA-1
Gene Sequence

>Msa1398370

ATGGGAGTACAGGGTACCCTAGAATACTTGTCTGATTTACTCAGTAGCACAAAAAAGAAAAAGAAGAAGCAAACTCAAACTGTATCTCTTAAAATCAGAATGGACTGTGAAGGTTGTGCTCGCAAGGTTAAGCATGTCCTTTCTGGTGTTAAAGGAGCTAAGAAAGTGGATGTGGACTTGAAGCAGCAGAAAGTAACAGTGAGTGGATATGTTGAACCAAAGAAAGTGTTGAAAGCTGCTCAATCAACAAAGAAGAAGGTTGAGCTTTGGCCTTATGTTCCATACACTATGGTGGCTCACCCTTATATTTCACAAGCCTATGATAAGAAAGCACCTCCTAATATGGTTAGAAAGGTTGCTGACACTTCCAACATAAAAGAGTCCACTTTTGATGATAGCTATGTTGAAATGTTCAGTGATGAAAATCCAAATGCTTGTTCTATTATGTAG
Protein sequence

>Msa1398370

MGVQGTLEYLSDLLSSTKKKKKKQTQTVSLKIRMDCEGCARKVKHVLSGVKGAKKVDVDLKQQKVTVSGYVEPKKVLKAAQSTKKKVELWPYVPYTMVAHPYISQAYDKKAPPNMVRKVADTSNIKESTFDDSYVEMFSDENPNACSIM*