AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0469690

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0469690 MtrunA17_Chr3g0124861 95.397 239 7 1 1 239 1 235 1.49e-163 451
Msa0469690 MtrunA17_Chr8g0352871 54.494 178 66 2 19 195 6 169 2.38e-59 185
Msa0469690 MtrunA17_Chr3g0082861 59.477 153 48 2 22 173 4 143 2.05e-58 182
Msa0469690 MtrunA17_Chr7g0256551 54.706 170 58 5 22 186 9 164 3.01e-54 174
Msa0469690 MtrunA17_Chr5g0414931 45.213 188 83 5 20 206 14 182 8.09e-49 160
Msa0469690 MtrunA17_Chr3g0128691 53.472 144 48 2 23 166 19 143 3.33e-48 156
Msa0469690 MtrunA17_Chr3g0118211 50.649 154 53 3 21 173 22 153 9.24e-48 156
Msa0469690 MtrunA17_Chr1g0170741 53.289 152 51 4 20 170 6 138 2.58e-46 152
Msa0469690 MtrunA17_Chr4g0059571 49.351 154 55 3 21 173 25 156 1.04e-44 149
Msa0469690 MtrunA17_Chr4g0021731 47.573 103 32 2 19 121 8 88 1.43e-25 95.9
Msa0469690 MtrunA17_Chr1g0181811 31.034 116 79 1 99 213 131 246 5.65e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0469690 AT2G41310.1 58.170 153 49 3 22 173 9 147 4.92e-59 186
Msa0469690 AT3G57040.2 57.051 156 54 3 19 173 6 149 1.85e-56 180
Msa0469690 AT3G57040.1 57.051 156 54 3 19 173 6 149 1.85e-56 180
Msa0469690 AT1G59940.1 47.647 170 66 3 11 179 22 169 4.91e-49 161
Msa0469690 AT3G48100.1 51.592 157 54 3 22 177 25 160 1.50e-47 155
Msa0469690 AT1G59940.2 47.647 170 66 3 11 179 114 261 2.11e-47 159
Msa0469690 AT1G10470.3 50.000 162 58 3 17 177 15 154 4.62e-47 156
Msa0469690 AT5G62920.1 53.947 152 47 3 22 172 25 154 6.86e-47 154
Msa0469690 AT1G10470.1 50.000 162 58 3 17 177 29 168 6.88e-47 156
Msa0469690 AT3G56380.1 51.299 154 53 3 19 171 17 149 2.39e-45 149
Msa0469690 AT1G19050.1 50.327 153 53 3 21 172 23 153 3.15e-45 150
Msa0469690 AT3G56380.2 50.323 155 54 3 19 171 17 150 7.37e-45 147
Msa0469690 AT1G74890.1 48.718 156 57 3 21 175 17 150 2.23e-44 148
Msa0469690 AT2G40670.1 46.835 158 54 3 20 171 27 160 1.25e-41 140
Msa0469690 AT2G40670.2 45.912 159 55 3 20 171 27 161 2.78e-41 139
Msa0469690 AT2G07440.1 53.846 52 24 0 122 173 10 61 8.64e-15 69.3
Msa0469690 AT1G10470.2 37.805 82 50 1 92 172 6 87 1.31e-13 67.4

Find 71 sgRNAs with CRISPR-Local

Find 204 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACCGCCAACCCTTTCATTT+TGG 0.085264 3_3:+71653573 None:intergenic
TGTGAGCACGTACTAATATT+AGG 0.212614 3_3:-71653463 Msa0469690:three_prime_UTR
CTGCACTGCAGGGATCTTCT+TGG 0.261594 3_3:-71654123 Msa0469690:intron
TCTATTTAATAGTAGTTATA+TGG 0.268250 3_3:+71653371 None:intergenic
TCTCTCATTCCCCTTTCAAT+TGG 0.305421 3_3:+71657259 None:intergenic
GATAAGGCTTTGAAATATCT+TGG 0.316965 3_3:-71656626 Msa0469690:CDS
TCTGATGACATAACCACAAC+TGG 0.369928 3_3:+71654091 None:intergenic
AATTGATATGGAGCTTGCTT+TGG 0.375819 3_3:-71656952 Msa0469690:exon
AAGCAAGCTCCATATCAATT+TGG 0.381935 3_3:+71656955 None:intergenic
TATTCATCACCAAATTGATA+TGG 0.391096 3_3:-71656964 Msa0469690:exon
CAAATAAAATCCAATTGAAA+GGG 0.426510 3_3:-71657269 Msa0469690:five_prime_UTR
ATTGATCTCTCTGGAGGCTC+AGG 0.433469 3_3:+71653598 None:intergenic
GTCCGAGTCTGTGGTGGTAG+TGG 0.436880 3_3:+71653702 None:intergenic
GGGAAGATGATGAATGAGAG+TGG 0.441227 3_3:+71657050 None:intergenic
GTGAGCACGTACTAATATTA+GGG 0.443815 3_3:-71653462 Msa0469690:three_prime_UTR
ATGACAGATTATTGCATGCC+TGG 0.445954 3_3:-71655348 Msa0469690:CDS
TAGTAGCTTTATTCTATTAG+TGG 0.456713 3_3:+71657105 None:intergenic
AATGAAAGGGTTGGCGGTGG+TGG 0.458526 3_3:-71653569 Msa0469690:CDS
GCTAATAGGTGCTTGGAAGA+AGG 0.458769 3_3:-71653838 Msa0469690:intron
CAACGTGGTGGAGAGAATCT+AGG 0.465538 3_3:-71657212 Msa0469690:five_prime_UTR
GTATACAAGTAGTAGAAATC+AGG 0.467196 3_3:+71653393 None:intergenic
AGTAGCTTTATTCTATTAGT+GGG 0.472302 3_3:+71657106 None:intergenic
CTGTGTGGACTCTGGAGATA+AGG 0.474705 3_3:-71656642 Msa0469690:CDS
ACTTACATGCTAATTCTAGA+AGG 0.476558 3_3:+71654061 None:intergenic
TCAAATAAAATCCAATTGAA+AGG 0.478763 3_3:-71657270 Msa0469690:five_prime_UTR
ATTACCTCTTGTAGCTGCAA+TGG 0.489838 3_3:+71656521 None:intergenic
CTAATAGGTGCTTGGAAGAA+GGG 0.490036 3_3:-71653837 Msa0469690:intron
TAATTTGGCTAATAGGTGCT+TGG 0.497490 3_3:-71653845 Msa0469690:intron
ACAGTTACCTGTGTGGACTC+TGG 0.503672 3_3:-71656650 Msa0469690:intron
CTCTGGAGGCTCAGGAGACA+TGG 0.506463 3_3:+71653606 None:intergenic
CATCAGCATGATGACCCAAT+GGG 0.522832 3_3:-71657324 None:intergenic
TTGGAAAGATGTTCCAGTTG+TGG 0.523397 3_3:-71654104 Msa0469690:CDS
AGATCAATGCCCAAAATGAA+AGG 0.528945 3_3:-71653583 Msa0469690:CDS
CTTAGAGATTCTTCATGCAA+AGG 0.532173 3_3:-71656825 Msa0469690:intron
TAGATGATAGTGTCATTGAT+AGG 0.538094 3_3:-71656866 Msa0469690:CDS
TTGGGCATTGATCTCTCTGG+AGG 0.540639 3_3:+71653592 None:intergenic
AAATCATAGCCACTCATCCC+AGG 0.545060 3_3:+71655330 None:intergenic
GTAGTAGAAATCAGGTTTCG+TGG 0.547136 3_3:+71653401 None:intergenic
AACGTGGTGGAGAGAATCTA+GGG 0.557971 3_3:-71657211 Msa0469690:five_prime_UTR
GCCCAAAATGAAAGGGTTGG+CGG 0.559802 3_3:-71653575 Msa0469690:CDS
ACTGTAGTGGAACAGTGGTA+TGG 0.562212 3_3:+71657144 None:intergenic
TGCAAATCTGATAACTGAAG+AGG 0.563149 3_3:+71653793 None:intergenic
GCAAGAACACTTTCATGTGT+TGG 0.563382 3_3:-71656892 Msa0469690:CDS
TTCCACTACCACCACAGACT+CGG 0.564431 3_3:-71653704 Msa0469690:CDS
CCATCAGCATGATGACCCAA+TGG 0.567336 3_3:-71657325 None:intergenic
GATCAATGCCCAAAATGAAA+GGG 0.575333 3_3:-71653582 Msa0469690:CDS
TGACAGATTATTGCATGCCT+GGG 0.579945 3_3:-71655347 Msa0469690:CDS
TGCAATGGTTGTTGAAGAGG+AGG 0.579983 3_3:+71656536 None:intergenic
TGAGAGTGGGAAAAGAGAGG+TGG 0.584714 3_3:+71657064 None:intergenic
AGCTGCAATGGTTGTTGAAG+AGG 0.586818 3_3:+71656533 None:intergenic
ACATTAATAGTATCAGCAAA+CGG 0.588740 3_3:-71653633 Msa0469690:CDS
TAATAGGTGCTTGGAAGAAG+GGG 0.593866 3_3:-71653836 Msa0469690:intron
GTTGTCGTCGTCCGAGTCTG+TGG 0.598775 3_3:+71653693 None:intergenic
AATGCCCAAAATGAAAGGGT+TGG 0.602173 3_3:-71653578 Msa0469690:CDS
GGAAGATGATGAATGAGAGT+GGG 0.604844 3_3:+71657051 None:intergenic
TTGATAGGATGCTTCTAGAG+AGG 0.609363 3_3:-71656851 Msa0469690:CDS
TAATAGTATCAGCAAACGGA+AGG 0.609687 3_3:-71653629 Msa0469690:CDS
ACAACCATTGCAGCTACAAG+AGG 0.610346 3_3:-71656525 Msa0469690:intron
AAATAAAATCCAATTGAAAG+GGG 0.615171 3_3:-71657268 Msa0469690:five_prime_UTR
TATTGCATGCCTGGGATGAG+TGG 0.615464 3_3:-71655339 Msa0469690:CDS
CAAAATGAAAGGGTTGGCGG+TGG 0.616336 3_3:-71653572 Msa0469690:CDS
TGATTTACTTAAAAGAGTCA+AGG 0.620876 3_3:-71655313 Msa0469690:intron
GAATGAGAGTGGGAAAAGAG+AGG 0.639186 3_3:+71657061 None:intergenic
GTCGTCGTCCGAGTCTGTGG+TGG 0.650795 3_3:+71653696 None:intergenic
TAATATAGAAGACTTTGTTG+AGG 0.651761 3_3:+71656571 None:intergenic
AAAATCGTACAAATTAAGAG+TGG 0.659476 3_3:-71653537 Msa0469690:three_prime_UTR
TGTTAAAACAGTTACCTGTG+TGG 0.666037 3_3:-71656657 Msa0469690:intron
CTTATCTCCAGAGTCCACAC+AGG 0.676893 3_3:+71656643 None:intergenic
GAGTAGTTGTACCAACGTGG+TGG 0.683701 3_3:-71657224 Msa0469690:five_prime_UTR
CTAGATTCTCTCCACCACGT+TGG 0.728914 3_3:+71657213 None:intergenic
AAAGAGTAGTTGTACCAACG+TGG 0.745673 3_3:-71657227 Msa0469690:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATATATACATGTTATATA+TGG - chr3_3:71654647-71654666 Msa0469690:intron 10.0%
!!! ACATTTTTTTTTTTTTTTGA+AGG - chr3_3:71654892-71654911 Msa0469690:intron 10.0%
!!! AACATTAAATTTTTATGTTT+GGG - chr3_3:71655277-71655296 Msa0469690:intron 10.0%
!!! AAGTATTTAAGATATTATAA+AGG + chr3_3:71656723-71656742 None:intergenic 10.0%
!! ATTTAATTAACAACTTAATT+TGG - chr3_3:71656840-71656859 Msa0469690:CDS 10.0%
!!! TTAGCTTTATTAATTTTATG+GGG + chr3_3:71654402-71654421 None:intergenic 15.0%
!!! CTTAGCTTTATTAATTTTAT+GGG + chr3_3:71654403-71654422 None:intergenic 15.0%
!!! ATATATATTCTCTTGTTTAT+AGG - chr3_3:71655011-71655030 Msa0469690:intron 15.0%
!!! GAACATTAAATTTTTATGTT+TGG - chr3_3:71655276-71655295 Msa0469690:intron 15.0%
!!! AGAAAATTAATTTTACTTCA+AGG - chr3_3:71655581-71655600 Msa0469690:intron 15.0%
!!! TTTGATATTTGATTTTTAAG+TGG + chr3_3:71656207-71656226 None:intergenic 15.0%
!!! TAAATTAAATCCTAACTTTT+TGG - chr3_3:71656417-71656436 Msa0469690:intron 15.0%
!!! AAATTAAATCCTAACTTTTT+GGG - chr3_3:71656418-71656437 Msa0469690:intron 15.0%
!! TCAAAAGAAGAAAAAATTAA+TGG + chr3_3:71656548-71656567 None:intergenic 15.0%
!! TCAAATAAAATCCAATTGAA+AGG - chr3_3:71653430-71653449 Msa0469690:three_prime_UTR 20.0%
!! CAAATAAAATCCAATTGAAA+GGG - chr3_3:71653431-71653450 Msa0469690:three_prime_UTR 20.0%
!! AAATAAAATCCAATTGAAAG+GGG - chr3_3:71653432-71653451 Msa0469690:three_prime_UTR 20.0%
!!! GCTTAGCTTTATTAATTTTA+TGG + chr3_3:71654404-71654423 None:intergenic 20.0%
!! TGAATGAAAGTAATTTGATA+AGG + chr3_3:71654607-71654626 None:intergenic 20.0%
!! ATACATGTTATATATGGATA+TGG - chr3_3:71654653-71654672 Msa0469690:intron 20.0%
!! GTACTACATATATATGATAA+TGG - chr3_3:71654859-71654878 Msa0469690:intron 20.0%
!! GAAAAAAGAATACAATTCAA+TGG - chr3_3:71655063-71655082 Msa0469690:intron 20.0%
!!! TCTCTTTTTTAAACTTGAAT+AGG - chr3_3:71655453-71655472 Msa0469690:intron 20.0%
!!! TATACTTCTTTTTGAATTGT+TGG + chr3_3:71655862-71655881 None:intergenic 20.0%
!!! ACAATTCAAAAAGAAGTATA+AGG - chr3_3:71655862-71655881 Msa0469690:intron 20.0%
!!! CAATTCAAAAAGAAGTATAA+GGG - chr3_3:71655863-71655882 Msa0469690:intron 20.0%
!! AACTATTCAAAATACATACA+AGG - chr3_3:71655942-71655961 Msa0469690:intron 20.0%
!! ATGCAACAATTTAAAGATTT+GGG + chr3_3:71656077-71656096 None:intergenic 20.0%
!!! AGATAGATTTAGTTCATATA+TGG + chr3_3:71656382-71656401 None:intergenic 20.0%
!! ACAAAAAAAGAATAGATACA+TGG + chr3_3:71656521-71656540 None:intergenic 20.0%
!! AAATACTTCTTCAAAGAAAA+AGG - chr3_3:71656735-71656754 Msa0469690:intron 20.0%
!!! AGTAGCTTTATTCTATTAGT+GGG + chr3_3:71653597-71653616 None:intergenic 25.0%
!!! TAGTAGCTTTATTCTATTAG+TGG + chr3_3:71653598-71653617 None:intergenic 25.0%
! TATTCATCACCAAATTGATA+TGG - chr3_3:71653736-71653755 Msa0469690:CDS 25.0%
! TCCTAATAATTGAGATTGTA+CGG + chr3_3:71653924-71653943 None:intergenic 25.0%
!! AGAATTTTGTGTACTGAATT+TGG - chr3_3:71653988-71654007 Msa0469690:intron 25.0%
!!! TTGGTTAAGTAATTGTTTTG+TGG - chr3_3:71654007-71654026 Msa0469690:intron 25.0%
! TAATATAGAAGACTTTGTTG+AGG + chr3_3:71654132-71654151 None:intergenic 25.0%
!! AACTATCTTGAAAACTTTTG+AGG + chr3_3:71654229-71654248 None:intergenic 25.0%
!!! TTAGTTTTGTCCAGAAAAAA+AGG - chr3_3:71654279-71654298 Msa0469690:intron 25.0%
!!! AGCAAATATACCTTTTTTTC+TGG + chr3_3:71654292-71654311 None:intergenic 25.0%
! TAACAATTTCAACAGTCTAA+AGG + chr3_3:71654545-71654564 None:intergenic 25.0%
! ATGAAAGTAATTTGATAAGG+AGG + chr3_3:71654604-71654623 None:intergenic 25.0%
!!! ATGTTGATGAAGGTAATTTT+TGG + chr3_3:71654691-71654710 None:intergenic 25.0%
!! GGAATTATATATGTTGATGA+AGG + chr3_3:71654701-71654720 None:intergenic 25.0%
!!! CCTAACTGTATTTTTTTTAG+AGG + chr3_3:71654722-71654741 None:intergenic 25.0%
!! CCTCTAAAAAAAATACAGTT+AGG - chr3_3:71654719-71654738 Msa0469690:intron 25.0%
! CATATATGACTTATGATACA+TGG - chr3_3:71654951-71654970 Msa0469690:intron 25.0%
! AGCATACATTAAATCAGATT+AGG - chr3_3:71655041-71655060 Msa0469690:intron 25.0%
! CATGGATCAAAACAATAATT+AGG + chr3_3:71655145-71655164 None:intergenic 25.0%
!! AAATCTCTCTTTTGTGAAAA+AGG - chr3_3:71655220-71655239 Msa0469690:intron 25.0%
! TGATTTACTTAAAAGAGTCA+AGG - chr3_3:71655387-71655406 Msa0469690:intron 25.0%
! TCAAGTTTAAAAAAGAGAGA+GGG + chr3_3:71655451-71655470 None:intergenic 25.0%
!! TTCAAGTTTAAAAAAGAGAG+AGG + chr3_3:71655452-71655471 None:intergenic 25.0%
!! AATTAATTTTACTTCAAGGC+TGG - chr3_3:71655585-71655604 Msa0469690:intron 25.0%
!!! AGTTTAATTTTCGTATCTTG+TGG - chr3_3:71655646-71655665 Msa0469690:intron 25.0%
!! TGTGACTTTAAAGATAGTTA+TGG - chr3_3:71655706-71655725 Msa0469690:intron 25.0%
!! CATCCAATTTTATTAGACAT+AGG - chr3_3:71655813-71655832 Msa0469690:intron 25.0%
! TCTCCTATGTCTAATAAAAT+TGG + chr3_3:71655819-71655838 None:intergenic 25.0%
!!! TTGATTTAGAGTCTTTGTTT+TGG + chr3_3:71655900-71655919 None:intergenic 25.0%
! TAAATCAAGAATTGAGAAAG+AGG - chr3_3:71655912-71655931 Msa0469690:intron 25.0%
! AAATCAAGAATTGAGAAAGA+GGG - chr3_3:71655913-71655932 Msa0469690:intron 25.0%
! AACAATTTAAAGATTTGGGA+GGG + chr3_3:71656073-71656092 None:intergenic 25.0%
! CATGCAACAATTTAAAGATT+TGG + chr3_3:71656078-71656097 None:intergenic 25.0%
! TGGAATAAAAATCACTGAAA+TGG + chr3_3:71656117-71656136 None:intergenic 25.0%
!!! ATTTTTAAGTGGATCATAGT+TGG + chr3_3:71656196-71656215 None:intergenic 25.0%
! TTATTAATTGTAACTACCCA+AGG + chr3_3:71656291-71656310 None:intergenic 25.0%
! AGAAAGAGTAAGAAAGAAAA+AGG - chr3_3:71656442-71656461 Msa0469690:intron 25.0%
!! AACAACTTAATTTGGCTAAT+AGG - chr3_3:71656848-71656867 Msa0469690:CDS 25.0%
!!! AAGAATTTTCAAGGGATTTT+AGG + chr3_3:71656951-71656970 None:intergenic 25.0%
! ACATTAATAGTATCAGCAAA+CGG - chr3_3:71657067-71657086 Msa0469690:five_prime_UTR 25.0%
! AAAATCGTACAAATTAAGAG+TGG - chr3_3:71657163-71657182 Msa0469690:five_prime_UTR 25.0%
!! CAAGATTTGTTTTTCCCATT+GGG + chr3_3:71653393-71653412 None:intergenic 30.0%
!! TCAAGATTTGTTTTTCCCAT+TGG + chr3_3:71653394-71653413 None:intergenic 30.0%
! TAGATGATAGTGTCATTGAT+AGG - chr3_3:71653834-71653853 Msa0469690:intron 30.0%
AGATTGTACGGAAAAAATGT+TGG + chr3_3:71653912-71653931 None:intergenic 30.0%
TCCGTACAATCTCAATTATT+AGG - chr3_3:71653920-71653939 Msa0469690:intron 30.0%
!! GATAAGGCTTTGAAATATCT+TGG - chr3_3:71654074-71654093 Msa0469690:CDS 30.0%
!! TATCTTGAAAACTTTTGAGG+AGG + chr3_3:71654226-71654245 None:intergenic 30.0%
! TCCTTTTTCCTTTCTTAGTT+AGG - chr3_3:71654477-71654496 Msa0469690:intron 30.0%
TCCTAACTAAGAAAGGAAAA+AGG + chr3_3:71654481-71654500 None:intergenic 30.0%
TCTTAAGTCCTAACTAAGAA+AGG + chr3_3:71654488-71654507 None:intergenic 30.0%
TTAGAAGAAAAAAACGCAGA+AGG + chr3_3:71654517-71654536 None:intergenic 30.0%
TTAGTAGTATTATGCTGAGT+GGG - chr3_3:71654562-71654581 Msa0469690:intron 30.0%
TAAACCAAAGCGACTTTATA+TGG - chr3_3:71654767-71654786 Msa0469690:intron 30.0%
TTTGTGAAAAAGGATACGTA+CGG - chr3_3:71655230-71655249 Msa0469690:intron 30.0%
AAAAAGAGAGAGGGAATTTA+GGG + chr3_3:71655442-71655461 None:intergenic 30.0%
AAAAAAGAGAGAGGGAATTT+AGG + chr3_3:71655443-71655462 None:intergenic 30.0%
!!! TTTAGAGTCTTTGTTTTGGT+GGG + chr3_3:71655896-71655915 None:intergenic 30.0%
!!! ATTTAGAGTCTTTGTTTTGG+TGG + chr3_3:71655897-71655916 None:intergenic 30.0%
! TTTTTCCTCAAAGAACATGA+AGG + chr3_3:71656035-71656054 None:intergenic 30.0%
CAACAATTTAAAGATTTGGG+AGG + chr3_3:71656074-71656093 None:intergenic 30.0%
! TGATTTTTATTCCACGACAA+AGG - chr3_3:71656123-71656142 Msa0469690:intron 30.0%
!! TTATTTTTCATCCTTTGTCG+TGG + chr3_3:71656137-71656156 None:intergenic 30.0%
! TAGATGTAATTTTGTCGACA+AGG + chr3_3:71656230-71656249 None:intergenic 30.0%
ATGATGAAACATGAGGTTAT+GGG + chr3_3:71656265-71656284 None:intergenic 30.0%
TATGATGAAACATGAGGTTA+TGG + chr3_3:71656266-71656285 None:intergenic 30.0%
ACTTACATGCTAATTCTAGA+AGG + chr3_3:71656642-71656661 None:intergenic 30.0%
! ATTGATGCAGCATTAGATTT+TGG + chr3_3:71656789-71656808 None:intergenic 30.0%
! CTCATCAGAAGAATTTTCAA+GGG + chr3_3:71656959-71656978 None:intergenic 30.0%
GTATACAAGTAGTAGAAATC+AGG + chr3_3:71657310-71657329 None:intergenic 30.0%
! AAAAAACTGTAGTGGAACAG+TGG + chr3_3:71653564-71653583 None:intergenic 35.0%
CATGAGAGAAAAAACTGTAG+TGG + chr3_3:71653572-71653591 None:intergenic 35.0%
TAAGTATAGAGAGAGAGAGA+GGG + chr3_3:71653674-71653693 None:intergenic 35.0%
AAGCAAGCTCCATATCAATT+TGG + chr3_3:71653748-71653767 None:intergenic 35.0%
! AATTGATATGGAGCTTGCTT+TGG - chr3_3:71653748-71653767 Msa0469690:CDS 35.0%
!! TGTTCTTGCTTCAAGTTTTG+TGG + chr3_3:71653797-71653816 None:intergenic 35.0%
CTTAGAGATTCTTCATGCAA+AGG - chr3_3:71653875-71653894 Msa0469690:intron 35.0%
TTCTTCATGCAAAGGTAGTT+AGG - chr3_3:71653883-71653902 Msa0469690:intron 35.0%
TGTTAAAACAGTTACCTGTG+TGG - chr3_3:71654043-71654062 Msa0469690:intron 35.0%
AGTCAACACGATAAAATGGA+TGG + chr3_3:71654453-71654472 None:intergenic 35.0%
GCTTAGTCAACACGATAAAA+TGG + chr3_3:71654457-71654476 None:intergenic 35.0%
GTTAGTAGTATTATGCTGAG+TGG - chr3_3:71654561-71654580 Msa0469690:intron 35.0%
TAGTAGTATTATGCTGAGTG+GGG - chr3_3:71654563-71654582 Msa0469690:intron 35.0%
AACTCCATATAAAGTCGCTT+TGG + chr3_3:71654774-71654793 None:intergenic 35.0%
CAAGAATATAGTGTCTGCTA+TGG - chr3_3:71655174-71655193 Msa0469690:intron 35.0%
! ATCATGTGCACTATTTTGCA+GGG - chr3_3:71655310-71655329 Msa0469690:intron 35.0%
TCATCAAAGTAACCTCTTAG+AGG + chr3_3:71655994-71656013 None:intergenic 35.0%
AAGGTGTATGATGAAACATG+AGG + chr3_3:71656272-71656291 None:intergenic 35.0%
TCATGTTTCATCATACACCT+TGG - chr3_3:71656271-71656290 Msa0469690:intron 35.0%
CATGTTTCATCATACACCTT+GGG - chr3_3:71656272-71656291 Msa0469690:intron 35.0%
CTCTTTCTACCCAAAAAGTT+AGG + chr3_3:71656430-71656449 None:intergenic 35.0%
TAATTTGGCTAATAGGTGCT+TGG - chr3_3:71656855-71656874 Msa0469690:CDS 35.0%
TGCAAATCTGATAACTGAAG+AGG + chr3_3:71656910-71656929 None:intergenic 35.0%
!! TCAAGGGATTTTAGGAAGTA+TGG + chr3_3:71656943-71656962 None:intergenic 35.0%
! GCTCATCAGAAGAATTTTCA+AGG + chr3_3:71656960-71656979 None:intergenic 35.0%
TAATAGTATCAGCAAACGGA+AGG - chr3_3:71657071-71657090 Msa0469690:five_prime_UTR 35.0%
AGATCAATGCCCAAAATGAA+AGG - chr3_3:71657117-71657136 Msa0469690:five_prime_UTR 35.0%
GATCAATGCCCAAAATGAAA+GGG - chr3_3:71657118-71657137 Msa0469690:five_prime_UTR 35.0%
TGTGAGCACGTACTAATATT+AGG - chr3_3:71657237-71657256 Msa0469690:five_prime_UTR 35.0%
GTGAGCACGTACTAATATTA+GGG - chr3_3:71657238-71657257 Msa0469690:five_prime_UTR 35.0%
TCTCTCATTCCCCTTTCAAT+TGG + chr3_3:71653444-71653463 None:intergenic 40.0%
AAAGAGTAGTTGTACCAACG+TGG - chr3_3:71653473-71653492 Msa0469690:three_prime_UTR 40.0%
GGAAGATGATGAATGAGAGT+GGG + chr3_3:71653652-71653671 None:intergenic 40.0%
AAGTATAGAGAGAGAGAGAG+GGG + chr3_3:71653673-71653692 None:intergenic 40.0%
GTAAGTATAGAGAGAGAGAG+AGG + chr3_3:71653675-71653694 None:intergenic 40.0%
GCAAGAACACTTTCATGTGT+TGG - chr3_3:71653808-71653827 Msa0469690:CDS 40.0%
! TTGATAGGATGCTTCTAGAG+AGG - chr3_3:71653849-71653868 Msa0469690:intron 40.0%
ATTACCTCTTGTAGCTGCAA+TGG + chr3_3:71654182-71654201 None:intergenic 40.0%
!! CTTGAAAACTTTTGAGGAGG+AGG + chr3_3:71654223-71654242 None:intergenic 40.0%
TGAGTGGGGATATTGAACTT+TGG - chr3_3:71654577-71654596 Msa0469690:intron 40.0%
GGAGGATCTGAAAGAGAAAT+TGG + chr3_3:71654798-71654817 None:intergenic 40.0%
TTCAGATCCTCCATTCATCT+AGG - chr3_3:71654806-71654825 Msa0469690:intron 40.0%
ATATTCTTGCGAGGAGATCA+TGG + chr3_3:71655163-71655182 None:intergenic 40.0%
! CATCATGTGCACTATTTTGC+AGG - chr3_3:71655309-71655328 Msa0469690:intron 40.0%
ATGACAGATTATTGCATGCC+TGG - chr3_3:71655352-71655371 Msa0469690:CDS 40.0%
TGACAGATTATTGCATGCCT+GGG - chr3_3:71655353-71655372 Msa0469690:CDS 40.0%
AGGGAATTTAGGGAAGATGT+CGG + chr3_3:71655432-71655451 None:intergenic 40.0%
AGTTCACTAGCACTTTGCAA+AGG - chr3_3:71655554-71655573 Msa0469690:intron 40.0%
AAAGAAAACTGCTTACGAGC+AGG - chr3_3:71655774-71655793 Msa0469690:intron 40.0%
TCAAAGTAACCTCTTAGAGG+TGG + chr3_3:71655991-71656010 None:intergenic 40.0%
TTCCACCTTCATGTTCTTTG+AGG - chr3_3:71656027-71656046 Msa0469690:intron 40.0%
TTCCTCAAAGAACATGAAGG+TGG + chr3_3:71656032-71656051 None:intergenic 40.0%
TTGGAAAGATGTTCCAGTTG+TGG - chr3_3:71656596-71656615 Msa0469690:CDS 40.0%
TCTGATGACATAACCACAAC+TGG + chr3_3:71656612-71656631 None:intergenic 40.0%
CTAATAGGTGCTTGGAAGAA+GGG - chr3_3:71656863-71656882 Msa0469690:CDS 40.0%
TAATAGGTGCTTGGAAGAAG+GGG - chr3_3:71656864-71656883 Msa0469690:CDS 40.0%
!!! ATTTTGGGCATTGATCTCTC+TGG + chr3_3:71657114-71657133 None:intergenic 40.0%
AATGCCCAAAATGAAAGGGT+TGG - chr3_3:71657122-71657141 Msa0469690:five_prime_UTR 40.0%
! ATTTTCGCGTGCATTTCTGT+CGG + chr3_3:71657278-71657297 None:intergenic 40.0%
GTAGTAGAAATCAGGTTTCG+TGG + chr3_3:71657302-71657321 None:intergenic 40.0%
! AACGTGGTGGAGAGAATCTA+GGG - chr3_3:71653489-71653508 Msa0469690:three_prime_UTR 45.0%
! ACTGTAGTGGAACAGTGGTA+TGG + chr3_3:71653559-71653578 None:intergenic 45.0%
GAATGAGAGTGGGAAAAGAG+AGG + chr3_3:71653642-71653661 None:intergenic 45.0%
GGGAAGATGATGAATGAGAG+TGG + chr3_3:71653653-71653672 None:intergenic 45.0%
TGCAATGGTTGTTGAAGAGG+AGG + chr3_3:71654167-71654186 None:intergenic 45.0%
AGCTGCAATGGTTGTTGAAG+AGG + chr3_3:71654170-71654189 None:intergenic 45.0%
ACAACCATTGCAGCTACAAG+AGG - chr3_3:71654175-71654194 Msa0469690:intron 45.0%
GTACGTACAGTGCAGTGAAA+AGG + chr3_3:71655121-71655140 None:intergenic 45.0%
GCAGACACTATATTCTTGCG+AGG + chr3_3:71655172-71655191 None:intergenic 45.0%
AAATCATAGCCACTCATCCC+AGG + chr3_3:71655373-71655392 None:intergenic 45.0%
GGAATTTAGGGAAGATGTCG+GGG + chr3_3:71655430-71655449 None:intergenic 45.0%
GGGAATTTAGGGAAGATGTC+GGG + chr3_3:71655431-71655450 None:intergenic 45.0%
AGCTAGAAACCACCTCTAAG+AGG - chr3_3:71655979-71655998 Msa0469690:intron 45.0%
GAAGGTGGAAGAGATTGTAG+AGG + chr3_3:71656017-71656036 None:intergenic 45.0%
GCTAATAGGTGCTTGGAAGA+AGG - chr3_3:71656862-71656881 Msa0469690:CDS 45.0%
! ACCGCCAACCCTTTCATTTT+GGG + chr3_3:71657129-71657148 None:intergenic 45.0%
! TTTTCGCGTGCATTTCTGTC+GGG + chr3_3:71657277-71657296 None:intergenic 45.0%
!!! TTTATTTTATATATTTTGAA+TGG - chr3_3:71655730-71655749 Msa0469690:intron 5.0%
GAGTAGTTGTACCAACGTGG+TGG - chr3_3:71653476-71653495 Msa0469690:three_prime_UTR 50.0%
CTAGATTCTCTCCACCACGT+TGG + chr3_3:71653490-71653509 None:intergenic 50.0%
! CAACGTGGTGGAGAGAATCT+AGG - chr3_3:71653488-71653507 Msa0469690:three_prime_UTR 50.0%
TGAGAGTGGGAAAAGAGAGG+TGG + chr3_3:71653639-71653658 None:intergenic 50.0%
ACAGTTACCTGTGTGGACTC+TGG - chr3_3:71654050-71654069 Msa0469690:intron 50.0%
CTTATCTCCAGAGTCCACAC+AGG + chr3_3:71654060-71654079 None:intergenic 50.0%
! CTGTGTGGACTCTGGAGATA+AGG - chr3_3:71654058-71654077 Msa0469690:intron 50.0%
TCCTCCATTCATCTAGGTCG+CGG - chr3_3:71654812-71654831 Msa0469690:intron 50.0%
TATTGCATGCCTGGGATGAG+TGG - chr3_3:71655361-71655380 Msa0469690:CDS 50.0%
ACACTGCTTACTGCACTGCA+GGG - chr3_3:71656567-71656586 Msa0469690:CDS 50.0%
TTCCACTACCACCACAGACT+CGG - chr3_3:71656996-71657015 Msa0469690:five_prime_UTR 50.0%
! ATTGATCTCTCTGGAGGCTC+AGG + chr3_3:71657105-71657124 None:intergenic 50.0%
!! TTGGGCATTGATCTCTCTGG+AGG + chr3_3:71657111-71657130 None:intergenic 50.0%
GCCCAAAATGAAAGGGTTGG+CGG - chr3_3:71657125-71657144 Msa0469690:five_prime_UTR 50.0%
! CACCGCCAACCCTTTCATTT+TGG + chr3_3:71657130-71657149 None:intergenic 50.0%
! CAAAATGAAAGGGTTGGCGG+TGG - chr3_3:71657128-71657147 Msa0469690:five_prime_UTR 50.0%
TCCGCGACCTAGATGAATGG+AGG + chr3_3:71654816-71654835 None:intergenic 55.0%
GCATCCGCGACCTAGATGAA+TGG + chr3_3:71654819-71654838 None:intergenic 55.0%
GACACTGCTTACTGCACTGC+AGG - chr3_3:71656566-71656585 Msa0469690:CDS 55.0%
CTGCACTGCAGGGATCTTCT+TGG - chr3_3:71656577-71656596 Msa0469690:CDS 55.0%
! AATGAAAGGGTTGGCGGTGG+TGG - chr3_3:71657131-71657150 Msa0469690:five_prime_UTR 55.0%
GTCCGAGTCTGTGGTGGTAG+TGG + chr3_3:71657001-71657020 None:intergenic 60.0%
GTTGTCGTCGTCCGAGTCTG+TGG + chr3_3:71657010-71657029 None:intergenic 60.0%
! CTCTGGAGGCTCAGGAGACA+TGG + chr3_3:71657097-71657116 None:intergenic 60.0%
GTCGTCGTCCGAGTCTGTGG+TGG + chr3_3:71657007-71657026 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 71653385 71657337 71653385 ID=Msa0469690;Name=Msa0469690
chr3_3 mRNA 71653385 71657337 71653385 ID=Msa0469690-mRNA-1;Parent=Msa0469690;Name=Msa0469690-mRNA-1;_AED=0.07;_eAED=0.07;_QI=370|1|1|1|1|1|5|179|239
chr3_3 exon 71653385 71653852 71653385 ID=Msa0469690-mRNA-1:exon:17633;Parent=Msa0469690-mRNA-1
chr3_3 exon 71654067 71654134 71654067 ID=Msa0469690-mRNA-1:exon:17632;Parent=Msa0469690-mRNA-1
chr3_3 exon 71655314 71655391 71655314 ID=Msa0469690-mRNA-1:exon:17631;Parent=Msa0469690-mRNA-1
chr3_3 exon 71656526 71656668 71656526 ID=Msa0469690-mRNA-1:exon:17630;Parent=Msa0469690-mRNA-1
chr3_3 exon 71656826 71657337 71656826 ID=Msa0469690-mRNA-1:exon:17629;Parent=Msa0469690-mRNA-1
chr3_3 five_prime_UTR 71656968 71657337 71656968 ID=Msa0469690-mRNA-1:five_prime_utr;Parent=Msa0469690-mRNA-1
chr3_3 CDS 71656826 71656967 71656826 ID=Msa0469690-mRNA-1:cds;Parent=Msa0469690-mRNA-1
chr3_3 CDS 71656526 71656668 71656526 ID=Msa0469690-mRNA-1:cds;Parent=Msa0469690-mRNA-1
chr3_3 CDS 71655314 71655391 71655314 ID=Msa0469690-mRNA-1:cds;Parent=Msa0469690-mRNA-1
chr3_3 CDS 71654067 71654134 71654067 ID=Msa0469690-mRNA-1:cds;Parent=Msa0469690-mRNA-1
chr3_3 CDS 71653564 71653852 71653564 ID=Msa0469690-mRNA-1:cds;Parent=Msa0469690-mRNA-1
chr3_3 three_prime_UTR 71653385 71653563 71653385 ID=Msa0469690-mRNA-1:three_prime_utr;Parent=Msa0469690-mRNA-1
Gene Sequence

>Msa0469690

ATGGAGCTTGCTTTGGATAGTAGAAAATTACAATTACAACCACAAAACTTGAAGCAAGAACACTTTCATGTGTTGGCTGTAGATGATAGTGTCATTGATAGGATGCTTCTAGAGAGGCTTCTTAGAGATTCTTCATGCAAAGTTACCTGTGTGGACTCTGGAGATAAGGCTTTGAAATATCTTGGATTGAATATTGATGAAATTGATAATAATTCTTCCTCAACAAAGTCTTCTATATTAGAATCATCATCTCCTCCTCTTCAACAACCATTGCAGCTACAAGAGGGAAGCAAAGTGAATTTGATCATGACAGATTATTGCATGCCTGGGATGAGTGGCTATGATTTACTTAAAAGAGTCAAGGGATCTTCTTGGAAAGATGTTCCAGTTGTGGTTATGTCATCAGAAAATGTACCTTCTAGAATTAGCATGTGCTTGGAAGAAGGGGCAGAAGAGTTTCTAATGAAGCCTCTTCAGTTATCAGATTTGCAGAAGCTTCAACCATACTTCCTAAAATCCCTTGAAAATTCTTCTGATGAGCAAGACTCTGCTAACAGTTCCACTACCACCACAGACTCGGACGACGACAACGATCTCATCGACAGCAACAATAACAATAATGATGATAACATTAATAGTATCAGCAAACGGAAGGCCATGTCTCCTGAGCCTCCAGAGAGATCAATGCCCAAAATGAAAGGGTTGGCGGTGGTGGTGTAA
Protein sequence

>Msa0469690

MELALDSRKLQLQPQNLKQEHFHVLAVDDSVIDRMLLERLLRDSSCKVTCVDSGDKALKYLGLNIDEIDNNSSSTKSSILESSSPPLQQPLQLQEGSKVNLIMTDYCMPGMSGYDLLKRVKGSSWKDVPVVVMSSENVPSRISMCLEEGAEEFLMKPLQLSDLQKLQPYFLKSLENSSDEQDSANSSTTTTDSDDDNDLIDSNNNNNDDNINSISKRKAMSPEPPERSMPKMKGLAVVV*