AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0374410

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0374410 MtrunA17_Chr3g0124861 95.816 239 6 1 1 239 1 235 2.91e-164 453
Msa0374410 MtrunA17_Chr8g0352871 54.494 178 66 2 19 195 6 169 2.14e-59 185
Msa0374410 MtrunA17_Chr3g0082861 59.477 153 48 2 22 173 4 143 1.92e-58 182
Msa0374410 MtrunA17_Chr7g0256551 54.706 170 58 5 22 186 9 164 2.04e-54 174
Msa0374410 MtrunA17_Chr3g0128691 53.472 144 48 2 23 166 19 143 3.19e-48 156
Msa0374410 MtrunA17_Chr5g0414931 45.455 187 82 5 20 204 14 182 3.63e-48 159
Msa0374410 MtrunA17_Chr3g0118211 50.649 154 53 3 21 173 22 153 9.55e-48 156
Msa0374410 MtrunA17_Chr1g0170741 53.289 152 51 4 20 170 6 138 2.47e-46 152
Msa0374410 MtrunA17_Chr4g0059571 49.351 154 55 3 21 173 25 156 1.09e-44 149
Msa0374410 MtrunA17_Chr4g0021731 47.573 103 32 2 19 121 8 88 1.39e-25 96.3
Msa0374410 MtrunA17_Chr1g0181811 31.034 116 79 1 99 213 131 246 5.45e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0374410 AT2G41310.1 58.170 153 49 3 22 173 9 147 4.92e-59 186
Msa0374410 AT3G57040.2 57.051 156 54 3 19 173 6 149 1.95e-56 180
Msa0374410 AT3G57040.1 57.051 156 54 3 19 173 6 149 1.95e-56 180
Msa0374410 AT1G59940.1 47.647 170 66 3 11 179 22 169 4.55e-49 161
Msa0374410 AT3G48100.1 51.592 157 54 3 22 177 25 160 1.51e-47 155
Msa0374410 AT1G59940.2 47.647 170 66 3 11 179 114 261 1.86e-47 159
Msa0374410 AT1G10470.3 50.000 162 58 3 17 177 15 154 4.43e-47 156
Msa0374410 AT5G62920.1 53.947 152 47 3 22 172 25 154 6.29e-47 154
Msa0374410 AT1G10470.1 50.000 162 58 3 17 177 29 168 6.59e-47 156
Msa0374410 AT3G56380.1 51.299 154 53 3 19 171 17 149 2.50e-45 149
Msa0374410 AT1G19050.1 50.327 153 53 3 21 172 23 153 2.98e-45 150
Msa0374410 AT3G56380.2 50.323 155 54 3 19 171 17 150 7.37e-45 147
Msa0374410 AT1G74890.1 48.718 156 57 3 21 175 17 150 2.30e-44 148
Msa0374410 AT2G40670.1 46.835 158 54 3 20 171 27 160 1.31e-41 140
Msa0374410 AT2G40670.2 45.912 159 55 3 20 171 27 161 3.13e-41 139
Msa0374410 AT2G07440.1 53.846 52 24 0 122 173 10 61 8.37e-15 69.3
Msa0374410 AT1G10470.2 36.471 85 53 1 92 175 6 90 1.22e-13 67.4

Find 73 sgRNAs with CRISPR-Local

Find 203 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACCGCCAACCCTTTCATTT+TGG 0.085264 3_1:+73869800 None:intergenic
CTGCACTGCAGGGATCTTCT+TGG 0.261594 3_1:-73870350 Msa0374410:intron
TCTCTCATTCCCCTTTCAAT+TGG 0.305421 3_1:+73873487 None:intergenic
GATAAGGCTTTGAAATATCT+TGG 0.316965 3_1:-73872847 Msa0374410:CDS
TGCAATCTTTAAATTACTTT+GGG 0.329603 3_1:-73869569 None:intergenic
TTGCAATCTTTAAATTACTT+TGG 0.342091 3_1:-73869570 None:intergenic
TCTGATGACATAACCACAAC+TGG 0.369928 3_1:+73870318 None:intergenic
AATTGATATGGAGCTTGCTT+TGG 0.375819 3_1:-73873172 Msa0374410:exon
AAGCAAGCTCCATATCAATT+TGG 0.381935 3_1:+73873175 None:intergenic
TATTCATCACCAAATTGATA+TGG 0.391096 3_1:-73873184 Msa0374410:exon
CAAATAAAATCCAATTGAAA+GGG 0.426510 3_1:-73873497 Msa0374410:five_prime_UTR
CATTTGTACATAAATTTAAG+TGG 0.429695 3_1:+73869621 None:intergenic
ATTGATCTCTCTGGAGGCTC+AGG 0.433469 3_1:+73869825 None:intergenic
GTCCGAGTCTGTGGTGGTAG+TGG 0.436880 3_1:+73869929 None:intergenic
GGGAAGATGATGAATGAGAG+TGG 0.441227 3_1:+73873278 None:intergenic
ATGACAGATTATTGCATGCC+TGG 0.445954 3_1:-73871574 Msa0374410:CDS
TAGTAGCTTTATTCTATTAG+TGG 0.456713 3_1:+73873333 None:intergenic
AATGAAAGGGTTGGCGGTGG+TGG 0.458526 3_1:-73869796 Msa0374410:CDS
GCTAATAGGTGCTTGGAAGA+AGG 0.458769 3_1:-73870065 Msa0374410:intron
TGCAATATCTAGACGACAAA+GGG 0.464493 3_1:+73869588 None:intergenic
CAACGTGGTGGAGAGAATCT+AGG 0.465538 3_1:-73873440 Msa0374410:five_prime_UTR
AGTAGCTTTATTCTATTAGT+GGG 0.472302 3_1:+73873334 None:intergenic
CTGTGTGGACTCTGGAGATA+AGG 0.474705 3_1:-73872863 Msa0374410:CDS
ACTTACATGCTAATTCTAGA+AGG 0.476558 3_1:+73870288 None:intergenic
TCAAATAAAATCCAATTGAA+AGG 0.478763 3_1:-73873498 Msa0374410:five_prime_UTR
ATTACCTCTTGTAGCTGCAA+TGG 0.489838 3_1:+73872742 None:intergenic
CTAATAGGTGCTTGGAAGAA+GGG 0.490036 3_1:-73870064 Msa0374410:intron
TAATTTGGCTAATAGGTGCT+TGG 0.497490 3_1:-73870072 Msa0374410:intron
ACAGTTACCTGTGTGGACTC+TGG 0.503672 3_1:-73872871 Msa0374410:intron
CTCTGGAGGCTCAGGAGACA+TGG 0.506463 3_1:+73869833 None:intergenic
CATCAGCATGATGACCCAAT+GGG 0.522832 3_1:-73873552 None:intergenic
TTGGAAAGATGTTCCAGTTG+TGG 0.523397 3_1:-73870331 Msa0374410:CDS
AGATCAATGCCCAAAATGAA+AGG 0.528945 3_1:-73869810 Msa0374410:CDS
CTTAGAGATTCTTCATGCAA+AGG 0.532173 3_1:-73873045 Msa0374410:intron
TAGATGATAGTGTCATTGAT+AGG 0.538094 3_1:-73873086 Msa0374410:CDS
TTGGGCATTGATCTCTCTGG+AGG 0.540639 3_1:+73869819 None:intergenic
AAATCATAGCCACTCATCCC+AGG 0.545060 3_1:+73871556 None:intergenic
AACGTGGTGGAGAGAATCTA+GGG 0.557971 3_1:-73873439 Msa0374410:five_prime_UTR
GCCCAAAATGAAAGGGTTGG+CGG 0.559802 3_1:-73869802 Msa0374410:CDS
ACTGTAGTGGAACAGTGGTA+TGG 0.562212 3_1:+73873372 None:intergenic
TGCAAATCTGATAACTGAAG+AGG 0.563149 3_1:+73870020 None:intergenic
GCAAGAACACTTTCATGTGT+TGG 0.563382 3_1:-73873112 Msa0374410:CDS
TTCCACTACCACCACAGACT+CGG 0.564431 3_1:-73869931 Msa0374410:CDS
CCATCAGCATGATGACCCAA+TGG 0.567336 3_1:-73873553 None:intergenic
GATCAATGCCCAAAATGAAA+GGG 0.575333 3_1:-73869809 Msa0374410:CDS
TGACAGATTATTGCATGCCT+GGG 0.579945 3_1:-73871573 Msa0374410:CDS
TGCAATGGTTGTTGAAGAGG+AGG 0.579983 3_1:+73872757 None:intergenic
TGAGAGTGGGAAAAGAGAGG+TGG 0.584714 3_1:+73873292 None:intergenic
AGCTGCAATGGTTGTTGAAG+AGG 0.586818 3_1:+73872754 None:intergenic
TGTGGTGGTAGTGGAACTGT+TGG 0.587887 3_1:+73869938 None:intergenic
ACATTAATAGTATCAGCAAA+CGG 0.588740 3_1:-73869860 Msa0374410:CDS
TAATAGGTGCTTGGAAGAAG+GGG 0.593866 3_1:-73870063 Msa0374410:intron
GTTGTCGTCGTCCGAGTCTG+TGG 0.598775 3_1:+73869920 None:intergenic
AATGCCCAAAATGAAAGGGT+TGG 0.602173 3_1:-73869805 Msa0374410:CDS
GGAAGATGATGAATGAGAGT+GGG 0.604844 3_1:+73873279 None:intergenic
TTGATAGGATGCTTCTAGAG+AGG 0.609363 3_1:-73873071 Msa0374410:CDS
TAATAGTATCAGCAAACGGA+AGG 0.609687 3_1:-73869856 Msa0374410:CDS
ACAACCATTGCAGCTACAAG+AGG 0.610346 3_1:-73872746 Msa0374410:intron
AAATAAAATCCAATTGAAAG+GGG 0.615171 3_1:-73873496 Msa0374410:five_prime_UTR
TATTGCATGCCTGGGATGAG+TGG 0.615464 3_1:-73871565 Msa0374410:CDS
CAAAATGAAAGGGTTGGCGG+TGG 0.616336 3_1:-73869799 Msa0374410:CDS
TGATTTACTTAAAAGAGTCA+AGG 0.620876 3_1:-73871539 Msa0374410:intron
TTGCAATATCTAGACGACAA+AGG 0.621224 3_1:+73869587 None:intergenic
GAATGAGAGTGGGAAAAGAG+AGG 0.639186 3_1:+73873289 None:intergenic
ACATAAATTTAAGTGGCTAG+TGG 0.642603 3_1:+73869628 None:intergenic
GTCGTCGTCCGAGTCTGTGG+TGG 0.650795 3_1:+73869923 None:intergenic
TAATATAGAAGACTTTGTTG+AGG 0.651761 3_1:+73872792 None:intergenic
AAAATCGTACAAATTAAGAG+TGG 0.659476 3_1:-73869764 Msa0374410:three_prime_UTR
TGTTAAAACAGTTACCTGTG+TGG 0.666037 3_1:-73872878 Msa0374410:intron
CTTATCTCCAGAGTCCACAC+AGG 0.676893 3_1:+73872864 None:intergenic
GAGTAGTTGTACCAACGTGG+TGG 0.683701 3_1:-73873452 Msa0374410:five_prime_UTR
CTAGATTCTCTCCACCACGT+TGG 0.728914 3_1:+73873441 None:intergenic
AAAGAGTAGTTGTACCAACG+TGG 0.745673 3_1:-73873455 Msa0374410:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATATATACATGTTATATA+TGG - chr3_1:73870840-73870859 Msa0374410:intron 10.0%
!!! TATACATTTTTTTTTTTTGA+AGG - chr3_1:73871080-73871099 Msa0374410:intron 10.0%
!!! AACATTAAATTTTTATGTTT+GGG - chr3_1:73871465-73871484 Msa0374410:intron 10.0%
!!! AAGTATTTAAGATATAATAA+AGG + chr3_1:73872910-73872929 None:intergenic 10.0%
!! ATTTAATTAACAACTTAATT+TGG - chr3_1:73873027-73873046 Msa0374410:intron 10.0%
!!! TTAGCTTTATTAATTTTATG+GGG + chr3_1:73870595-73870614 None:intergenic 15.0%
!!! CTTAGCTTTATTAATTTTAT+GGG + chr3_1:73870596-73870615 None:intergenic 15.0%
!!! ATATATATTCTCTTGTTTAT+AGG - chr3_1:73871199-73871218 Msa0374410:intron 15.0%
!!! GAACATTAAATTTTTATGTT+TGG - chr3_1:73871464-73871483 Msa0374410:intron 15.0%
!!! AGAAAATTAATTTTACTTCA+AGG - chr3_1:73871769-73871788 Msa0374410:intron 15.0%
!!! TTTGATATTTGATTTTTAAG+TGG + chr3_1:73872394-73872413 None:intergenic 15.0%
!!! TAAATTAAATCCTAACATTT+TGG - chr3_1:73872604-73872623 Msa0374410:intron 15.0%
!!! AAATTAAATCCTAACATTTT+GGG - chr3_1:73872605-73872624 Msa0374410:intron 15.0%
!! TCAAAAGAAGAAAAAATTAA+TGG + chr3_1:73872735-73872754 None:intergenic 15.0%
!! TCAAATAAAATCCAATTGAA+AGG - chr3_1:73869616-73869635 Msa0374410:three_prime_UTR 20.0%
!! CAAATAAAATCCAATTGAAA+GGG - chr3_1:73869617-73869636 Msa0374410:three_prime_UTR 20.0%
!! AAATAAAATCCAATTGAAAG+GGG - chr3_1:73869618-73869637 Msa0374410:three_prime_UTR 20.0%
!!! GCTTAGCTTTATTAATTTTA+TGG + chr3_1:73870597-73870616 None:intergenic 20.0%
!! TGAATGAAAGTAATTTGATA+AGG + chr3_1:73870800-73870819 None:intergenic 20.0%
!! ATACATGTTATATATGGATA+TGG - chr3_1:73870846-73870865 Msa0374410:intron 20.0%
!! GTACTACATATATATGATAA+TGG - chr3_1:73871052-73871071 Msa0374410:intron 20.0%
!! GAAAAAAGAATACAATTCAA+TGG - chr3_1:73871251-73871270 Msa0374410:intron 20.0%
!!! TCTCTTTTTTAAACTTGAAT+AGG - chr3_1:73871641-73871660 Msa0374410:intron 20.0%
!!! TATACTTCTTTTTGAATTGT+TGG + chr3_1:73872049-73872068 None:intergenic 20.0%
!!! ACAATTCAAAAAGAAGTATA+AGG - chr3_1:73872049-73872068 Msa0374410:intron 20.0%
!!! CAATTCAAAAAGAAGTATAA+GGG - chr3_1:73872050-73872069 Msa0374410:intron 20.0%
!! AACTATTCAAAATACATACA+AGG - chr3_1:73872129-73872148 Msa0374410:intron 20.0%
!! ATGCAACAATTTAAAGATTT+GGG + chr3_1:73872264-73872283 None:intergenic 20.0%
!!! AGATAGATTTAGTTCATATA+TGG + chr3_1:73872569-73872588 None:intergenic 20.0%
!! ACAAAAAAAGAATAGATACA+TGG + chr3_1:73872708-73872727 None:intergenic 20.0%
!! AAATACTTCTTCAAAGAAAA+AGG - chr3_1:73872922-73872941 Msa0374410:intron 20.0%
!! CATTTGTACATAAATTTAAG+TGG + chr3_1:73873496-73873515 None:intergenic 20.0%
!!! AGTAGCTTTATTCTATTAGT+GGG + chr3_1:73869783-73869802 None:intergenic 25.0%
!!! TAGTAGCTTTATTCTATTAG+TGG + chr3_1:73869784-73869803 None:intergenic 25.0%
! TATTCATCACCAAATTGATA+TGG - chr3_1:73869930-73869949 Msa0374410:CDS 25.0%
! TCCTAATAATTGAGATTGTA+CGG + chr3_1:73870118-73870137 None:intergenic 25.0%
!! AGAATTTTGTGTACTGAATT+TGG - chr3_1:73870181-73870200 Msa0374410:intron 25.0%
!!! TTGGTTAAGTAATTGTTTTG+TGG - chr3_1:73870200-73870219 Msa0374410:intron 25.0%
! TAATATAGAAGACTTTGTTG+AGG + chr3_1:73870325-73870344 None:intergenic 25.0%
!! AACTATCTTGAAAACTTTTG+AGG + chr3_1:73870422-73870441 None:intergenic 25.0%
!!! TTAGTTTTGTCCAGAAAAAA+AGG - chr3_1:73870472-73870491 Msa0374410:intron 25.0%
!!! AGCAAATATACCTTTTTTTC+TGG + chr3_1:73870485-73870504 None:intergenic 25.0%
! TAACAATTTCAACAGTCTAA+AGG + chr3_1:73870738-73870757 None:intergenic 25.0%
! ATGAAAGTAATTTGATAAGG+AGG + chr3_1:73870797-73870816 None:intergenic 25.0%
!!! ATGTTGATGAAGGTAATTTT+TGG + chr3_1:73870884-73870903 None:intergenic 25.0%
!! GGAATTATATATGTTGATGA+AGG + chr3_1:73870894-73870913 None:intergenic 25.0%
!!! CCTAACTGTATTTTTTTTAG+AGG + chr3_1:73870915-73870934 None:intergenic 25.0%
!! CCTCTAAAAAAAATACAGTT+AGG - chr3_1:73870912-73870931 Msa0374410:intron 25.0%
! CATATATGACTTATGATACA+TGG - chr3_1:73871139-73871158 Msa0374410:intron 25.0%
! AGCATACATTAAATCAGATT+AGG - chr3_1:73871229-73871248 Msa0374410:intron 25.0%
! CATGGATCAAAACAATAATT+AGG + chr3_1:73871333-73871352 None:intergenic 25.0%
!! AAATCTCTCTTTTGTGAAAA+AGG - chr3_1:73871408-73871427 Msa0374410:intron 25.0%
! TGATTTACTTAAAAGAGTCA+AGG - chr3_1:73871575-73871594 Msa0374410:CDS 25.0%
! TCAAGTTTAAAAAAGAGAGA+GGG + chr3_1:73871639-73871658 None:intergenic 25.0%
!! TTCAAGTTTAAAAAAGAGAG+AGG + chr3_1:73871640-73871659 None:intergenic 25.0%
!! AATTAATTTTACTTCAAGGC+TGG - chr3_1:73871773-73871792 Msa0374410:intron 25.0%
!! TGTGACTTTAAAGATAGTTA+CGG - chr3_1:73871893-73871912 Msa0374410:intron 25.0%
!! CATCCAATTTTATTAGACAT+AGG - chr3_1:73872000-73872019 Msa0374410:intron 25.0%
! TCTCCTATGTCTAATAAAAT+TGG + chr3_1:73872006-73872025 None:intergenic 25.0%
!!! TTGATTTAGAGTCTTTGTTT+TGG + chr3_1:73872087-73872106 None:intergenic 25.0%
! TAAATCAAGAATTGAGAAAG+AGG - chr3_1:73872099-73872118 Msa0374410:intron 25.0%
! AAATCAAGAATTGAGAAAGA+GGG - chr3_1:73872100-73872119 Msa0374410:intron 25.0%
! AACAATTTAAAGATTTGGGA+GGG + chr3_1:73872260-73872279 None:intergenic 25.0%
! CATGCAACAATTTAAAGATT+TGG + chr3_1:73872265-73872284 None:intergenic 25.0%
! TGGAATAAAAATCACTGAAA+TGG + chr3_1:73872304-73872323 None:intergenic 25.0%
!!! ATTTTTAAGTGGATCATAGT+TGG + chr3_1:73872383-73872402 None:intergenic 25.0%
! TAATTAATTGTAACTACCCA+AGG + chr3_1:73872478-73872497 None:intergenic 25.0%
! AGAAAGAGTAAGAAAGAAAA+AGG - chr3_1:73872629-73872648 Msa0374410:intron 25.0%
!! AACAACTTAATTTGGCTAAT+AGG - chr3_1:73873035-73873054 Msa0374410:intron 25.0%
!!! AAGAATTTTCAAGGGATTTT+AGG + chr3_1:73873138-73873157 None:intergenic 25.0%
! ACATTAATAGTATCAGCAAA+CGG - chr3_1:73873254-73873273 Msa0374410:five_prime_UTR 25.0%
! AAAATCGTACAAATTAAGAG+TGG - chr3_1:73873350-73873369 Msa0374410:five_prime_UTR 25.0%
!! CAAGATTTGTTTTTCCCATT+GGG + chr3_1:73869579-73869598 None:intergenic 30.0%
!! TCAAGATTTGTTTTTCCCAT+TGG + chr3_1:73869580-73869599 None:intergenic 30.0%
! TAGATGATAGTGTCATTGAT+AGG - chr3_1:73870028-73870047 Msa0374410:CDS 30.0%
AGATTGTACGGAAAAAATGT+TGG + chr3_1:73870106-73870125 None:intergenic 30.0%
TCCGTACAATCTCAATTATT+AGG - chr3_1:73870114-73870133 Msa0374410:intron 30.0%
!! GATAAGGCTTTGAAATATCT+TGG - chr3_1:73870267-73870286 Msa0374410:intron 30.0%
!! TATCTTGAAAACTTTTGAGG+AGG + chr3_1:73870419-73870438 None:intergenic 30.0%
! TCCTTTTTCCTTTCTTAGTT+AGG - chr3_1:73870670-73870689 Msa0374410:intron 30.0%
TCCTAACTAAGAAAGGAAAA+AGG + chr3_1:73870674-73870693 None:intergenic 30.0%
TCTTAAGTCCTAACTAAGAA+AGG + chr3_1:73870681-73870700 None:intergenic 30.0%
TTAGAAGAAAAAAACGCAGA+AGG + chr3_1:73870710-73870729 None:intergenic 30.0%
TTAGTAGTATTATGCTGAGT+GGG - chr3_1:73870755-73870774 Msa0374410:intron 30.0%
TAAACCAAAGCGACTTTATA+TGG - chr3_1:73870960-73870979 Msa0374410:intron 30.0%
TTTGTGAAAAAGGATACGTA+CGG - chr3_1:73871418-73871437 Msa0374410:intron 30.0%
AAAAAGAGAGAGGGAATTTA+GGG + chr3_1:73871630-73871649 None:intergenic 30.0%
AAAAAAGAGAGAGGGAATTT+AGG + chr3_1:73871631-73871650 None:intergenic 30.0%
!! AGTTTAATTTTCGCATCTTG+TGG - chr3_1:73871833-73871852 Msa0374410:intron 30.0%
!!! TTTAGAGTCTTTGTTTTGGT+GGG + chr3_1:73872083-73872102 None:intergenic 30.0%
!!! ATTTAGAGTCTTTGTTTTGG+TGG + chr3_1:73872084-73872103 None:intergenic 30.0%
! TTTTTCCTCAAAGAACTTGA+AGG + chr3_1:73872222-73872241 None:intergenic 30.0%
CAACAATTTAAAGATTTGGG+AGG + chr3_1:73872261-73872280 None:intergenic 30.0%
! TGATTTTTATTCCACGACAA+AGG - chr3_1:73872310-73872329 Msa0374410:intron 30.0%
!! TTATTTTTCATCCTTTGTCG+TGG + chr3_1:73872324-73872343 None:intergenic 30.0%
! TAGATGTAATTTTGTCGACA+AGG + chr3_1:73872417-73872436 None:intergenic 30.0%
ATGATGAAACATGAGGTTAT+GGG + chr3_1:73872452-73872471 None:intergenic 30.0%
TATGATGAAACATGAGGTTA+TGG + chr3_1:73872453-73872472 None:intergenic 30.0%
ACTTACATGCTAATTCTAGA+AGG + chr3_1:73872829-73872848 None:intergenic 30.0%
! ATTGATGCAGCATTAGATTT+TGG + chr3_1:73872976-73872995 None:intergenic 30.0%
! CTCATCAGAAGAATTTTCAA+GGG + chr3_1:73873146-73873165 None:intergenic 30.0%
ACATAAATTTAAGTGGCTAG+TGG + chr3_1:73873489-73873508 None:intergenic 30.0%
! AAAAAACTGTAGTGGAACAG+TGG + chr3_1:73869750-73869769 None:intergenic 35.0%
CATGAGAGAAAAAACTGTAG+TGG + chr3_1:73869758-73869777 None:intergenic 35.0%
AAGCAAGCTCCATATCAATT+TGG + chr3_1:73869942-73869961 None:intergenic 35.0%
! AATTGATATGGAGCTTGCTT+TGG - chr3_1:73869942-73869961 Msa0374410:CDS 35.0%
!! TGTTCTTGCTTCAAGTTTTG+TGG + chr3_1:73869991-73870010 None:intergenic 35.0%
CTTAGAGATTCTTCATGCAA+AGG - chr3_1:73870069-73870088 Msa0374410:intron 35.0%
TTCTTCATGCAAAGGTAGTT+AGG - chr3_1:73870077-73870096 Msa0374410:intron 35.0%
TGTTAAAACAGTTACCTGTG+TGG - chr3_1:73870236-73870255 Msa0374410:intron 35.0%
AGTCAACACGATAAAATGGA+TGG + chr3_1:73870646-73870665 None:intergenic 35.0%
GCTTAGTCAACACGATAAAA+TGG + chr3_1:73870650-73870669 None:intergenic 35.0%
GTTAGTAGTATTATGCTGAG+TGG - chr3_1:73870754-73870773 Msa0374410:intron 35.0%
TAGTAGTATTATGCTGAGTG+GGG - chr3_1:73870756-73870775 Msa0374410:intron 35.0%
AACTCCATATAAAGTCGCTT+TGG + chr3_1:73870967-73870986 None:intergenic 35.0%
CAAGAATATAGTGTCTGCTA+TGG - chr3_1:73871362-73871381 Msa0374410:intron 35.0%
! ATCATGTGCACTATTTTGCA+GGG - chr3_1:73871498-73871517 Msa0374410:intron 35.0%
TCATCAAAGTAACCTCTTAG+AGG + chr3_1:73872181-73872200 None:intergenic 35.0%
AAGGTGTATGATGAAACATG+AGG + chr3_1:73872459-73872478 None:intergenic 35.0%
TCATGTTTCATCATACACCT+TGG - chr3_1:73872458-73872477 Msa0374410:intron 35.0%
CATGTTTCATCATACACCTT+GGG - chr3_1:73872459-73872478 Msa0374410:intron 35.0%
CTCTTTCTACCCAAAATGTT+AGG + chr3_1:73872617-73872636 None:intergenic 35.0%
TAATTTGGCTAATAGGTGCT+TGG - chr3_1:73873042-73873061 Msa0374410:intron 35.0%
TGCAAATCTGATAACTGAAG+AGG + chr3_1:73873097-73873116 None:intergenic 35.0%
!! TCAAGGGATTTTAGGAAGTA+TGG + chr3_1:73873130-73873149 None:intergenic 35.0%
! GCTCATCAGAAGAATTTTCA+AGG + chr3_1:73873147-73873166 None:intergenic 35.0%
TAATAGTATCAGCAAACGGA+AGG - chr3_1:73873258-73873277 Msa0374410:five_prime_UTR 35.0%
AGATCAATGCCCAAAATGAA+AGG - chr3_1:73873304-73873323 Msa0374410:five_prime_UTR 35.0%
GATCAATGCCCAAAATGAAA+GGG - chr3_1:73873305-73873324 Msa0374410:five_prime_UTR 35.0%
TGCAATATCTAGACGACAAA+GGG + chr3_1:73873529-73873548 None:intergenic 35.0%
TTGCAATATCTAGACGACAA+AGG + chr3_1:73873530-73873549 None:intergenic 35.0%
TCTCTCATTCCCCTTTCAAT+TGG + chr3_1:73869630-73869649 None:intergenic 40.0%
AAAGAGTAGTTGTACCAACG+TGG - chr3_1:73869659-73869678 Msa0374410:three_prime_UTR 40.0%
GGAAGATGATGAATGAGAGT+GGG + chr3_1:73869838-73869857 None:intergenic 40.0%
GCAAGAACACTTTCATGTGT+TGG - chr3_1:73870002-73870021 Msa0374410:CDS 40.0%
! TTGATAGGATGCTTCTAGAG+AGG - chr3_1:73870043-73870062 Msa0374410:CDS 40.0%
ATTACCTCTTGTAGCTGCAA+TGG + chr3_1:73870375-73870394 None:intergenic 40.0%
!! CTTGAAAACTTTTGAGGAGG+AGG + chr3_1:73870416-73870435 None:intergenic 40.0%
TGAGTGGGGATATTGAACTT+TGG - chr3_1:73870770-73870789 Msa0374410:intron 40.0%
GGAGGATCTGAAAGAGAAAT+TGG + chr3_1:73870991-73871010 None:intergenic 40.0%
TTCAGATCCTCCATTCATCT+AGG - chr3_1:73870999-73871018 Msa0374410:intron 40.0%
ATATTCTTGCGAGGAGATCA+TGG + chr3_1:73871351-73871370 None:intergenic 40.0%
! CATCATGTGCACTATTTTGC+AGG - chr3_1:73871497-73871516 Msa0374410:intron 40.0%
ATGACAGATTATTGCATGCC+TGG - chr3_1:73871540-73871559 Msa0374410:CDS 40.0%
TGACAGATTATTGCATGCCT+GGG - chr3_1:73871541-73871560 Msa0374410:CDS 40.0%
AGGGAATTTAGGGAAGATGT+CGG + chr3_1:73871620-73871639 None:intergenic 40.0%
AGTTCACTAGCACTTTGCAA+AGG - chr3_1:73871742-73871761 Msa0374410:intron 40.0%
AAAGAAAACTGCTTACGAGC+AGG - chr3_1:73871961-73871980 Msa0374410:intron 40.0%
TCAAAGTAACCTCTTAGAGG+TGG + chr3_1:73872178-73872197 None:intergenic 40.0%
! TTCCACCTTCAAGTTCTTTG+AGG - chr3_1:73872214-73872233 Msa0374410:intron 40.0%
TTCCTCAAAGAACTTGAAGG+TGG + chr3_1:73872219-73872238 None:intergenic 40.0%
TTGGAAAGATGTTCCAGTTG+TGG - chr3_1:73872783-73872802 Msa0374410:CDS 40.0%
TCTGATGACATAACCACAAC+TGG + chr3_1:73872799-73872818 None:intergenic 40.0%
CTAATAGGTGCTTGGAAGAA+GGG - chr3_1:73873050-73873069 Msa0374410:CDS 40.0%
TAATAGGTGCTTGGAAGAAG+GGG - chr3_1:73873051-73873070 Msa0374410:CDS 40.0%
!!! ATTTTGGGCATTGATCTCTC+TGG + chr3_1:73873301-73873320 None:intergenic 40.0%
AATGCCCAAAATGAAAGGGT+TGG - chr3_1:73873309-73873328 Msa0374410:five_prime_UTR 40.0%
! AACGTGGTGGAGAGAATCTA+GGG - chr3_1:73869675-73869694 Msa0374410:three_prime_UTR 45.0%
! ACTGTAGTGGAACAGTGGTA+TGG + chr3_1:73869745-73869764 None:intergenic 45.0%
GAATGAGAGTGGGAAAAGAG+AGG + chr3_1:73869828-73869847 None:intergenic 45.0%
GGGAAGATGATGAATGAGAG+TGG + chr3_1:73869839-73869858 None:intergenic 45.0%
TGCAATGGTTGTTGAAGAGG+AGG + chr3_1:73870360-73870379 None:intergenic 45.0%
AGCTGCAATGGTTGTTGAAG+AGG + chr3_1:73870363-73870382 None:intergenic 45.0%
ACAACCATTGCAGCTACAAG+AGG - chr3_1:73870368-73870387 Msa0374410:intron 45.0%
GTACGTACAGTGCAGTGAAA+AGG + chr3_1:73871309-73871328 None:intergenic 45.0%
GCAGACACTATATTCTTGCG+AGG + chr3_1:73871360-73871379 None:intergenic 45.0%
AAATCATAGCCACTCATCCC+AGG + chr3_1:73871561-73871580 None:intergenic 45.0%
GGAATTTAGGGAAGATGTCG+GGG + chr3_1:73871618-73871637 None:intergenic 45.0%
GGGAATTTAGGGAAGATGTC+GGG + chr3_1:73871619-73871638 None:intergenic 45.0%
AGCTAGAAACCACCTCTAAG+AGG - chr3_1:73872166-73872185 Msa0374410:intron 45.0%
GAAGGTGGAAGAGATTGTAG+AGG + chr3_1:73872204-73872223 None:intergenic 45.0%
GCTAATAGGTGCTTGGAAGA+AGG - chr3_1:73873049-73873068 Msa0374410:CDS 45.0%
! ACCGCCAACCCTTTCATTTT+GGG + chr3_1:73873316-73873335 None:intergenic 45.0%
!!! TTTATTTTATATATTTTGAA+TGG - chr3_1:73871917-73871936 Msa0374410:intron 5.0%
GAGTAGTTGTACCAACGTGG+TGG - chr3_1:73869662-73869681 Msa0374410:three_prime_UTR 50.0%
CTAGATTCTCTCCACCACGT+TGG + chr3_1:73869676-73869695 None:intergenic 50.0%
! CAACGTGGTGGAGAGAATCT+AGG - chr3_1:73869674-73869693 Msa0374410:three_prime_UTR 50.0%
TGAGAGTGGGAAAAGAGAGG+TGG + chr3_1:73869825-73869844 None:intergenic 50.0%
AGAGAGAGAGAGAGAGAGAG+GGG + chr3_1:73869859-73869878 None:intergenic 50.0%
GAGAGAGAGAGAGAGAGAGA+GGG + chr3_1:73869860-73869879 None:intergenic 50.0%
AGAGAGAGAGAGAGAGAGAG+AGG + chr3_1:73869859-73869878 None:intergenic 50.0%
ACAGTTACCTGTGTGGACTC+TGG - chr3_1:73870243-73870262 Msa0374410:intron 50.0%
CTTATCTCCAGAGTCCACAC+AGG + chr3_1:73870253-73870272 None:intergenic 50.0%
! CTGTGTGGACTCTGGAGATA+AGG - chr3_1:73870251-73870270 Msa0374410:intron 50.0%
TCCTCCATTCATCTAGGTCG+CGG - chr3_1:73871005-73871024 Msa0374410:intron 50.0%
TATTGCATGCCTGGGATGAG+TGG - chr3_1:73871549-73871568 Msa0374410:CDS 50.0%
ACACTGCTTACTGCACTGCA+GGG - chr3_1:73872754-73872773 Msa0374410:CDS 50.0%
!! TGTGGTGGTAGTGGAACTGT+TGG + chr3_1:73873179-73873198 None:intergenic 50.0%
TTCCACTACCACCACAGACT+CGG - chr3_1:73873183-73873202 Msa0374410:exon 50.0%
! ATTGATCTCTCTGGAGGCTC+AGG + chr3_1:73873292-73873311 None:intergenic 50.0%
!! TTGGGCATTGATCTCTCTGG+AGG + chr3_1:73873298-73873317 None:intergenic 50.0%
GCCCAAAATGAAAGGGTTGG+CGG - chr3_1:73873312-73873331 Msa0374410:five_prime_UTR 50.0%
! CACCGCCAACCCTTTCATTT+TGG + chr3_1:73873317-73873336 None:intergenic 50.0%
! CAAAATGAAAGGGTTGGCGG+TGG - chr3_1:73873315-73873334 Msa0374410:five_prime_UTR 50.0%
TCCGCGACCTAGATGAATGG+AGG + chr3_1:73871009-73871028 None:intergenic 55.0%
GCATCCGCGACCTAGATGAA+TGG + chr3_1:73871012-73871031 None:intergenic 55.0%
GACACTGCTTACTGCACTGC+AGG - chr3_1:73872753-73872772 Msa0374410:CDS 55.0%
CTGCACTGCAGGGATCTTCT+TGG - chr3_1:73872764-73872783 Msa0374410:CDS 55.0%
! AATGAAAGGGTTGGCGGTGG+TGG - chr3_1:73873318-73873337 Msa0374410:five_prime_UTR 55.0%
GTCCGAGTCTGTGGTGGTAG+TGG + chr3_1:73873188-73873207 None:intergenic 60.0%
GTTGTCGTCGTCCGAGTCTG+TGG + chr3_1:73873197-73873216 None:intergenic 60.0%
! CTCTGGAGGCTCAGGAGACA+TGG + chr3_1:73873284-73873303 None:intergenic 60.0%
GTCGTCGTCCGAGTCTGTGG+TGG + chr3_1:73873194-73873213 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 73869571 73873565 73869571 ID=Msa0374410;Name=Msa0374410
chr3_1 mRNA 73869571 73873565 73869571 ID=Msa0374410-mRNA-1;Parent=Msa0374410;Name=Msa0374410-mRNA-1;_AED=0.11;_eAED=0.11;_QI=378|1|1|1|1|1|5|220|239
chr3_1 exon 73869571 73870079 73869571 ID=Msa0374410-mRNA-1:exon:16882;Parent=Msa0374410-mRNA-1
chr3_1 exon 73870294 73870361 73870294 ID=Msa0374410-mRNA-1:exon:16881;Parent=Msa0374410-mRNA-1
chr3_1 exon 73871540 73871617 73871540 ID=Msa0374410-mRNA-1:exon:16880;Parent=Msa0374410-mRNA-1
chr3_1 exon 73872747 73872889 73872747 ID=Msa0374410-mRNA-1:exon:16879;Parent=Msa0374410-mRNA-1
chr3_1 exon 73873046 73873565 73873046 ID=Msa0374410-mRNA-1:exon:16878;Parent=Msa0374410-mRNA-1
chr3_1 five_prime_UTR 73873188 73873565 73873188 ID=Msa0374410-mRNA-1:five_prime_utr;Parent=Msa0374410-mRNA-1
chr3_1 CDS 73873046 73873187 73873046 ID=Msa0374410-mRNA-1:cds;Parent=Msa0374410-mRNA-1
chr3_1 CDS 73872747 73872889 73872747 ID=Msa0374410-mRNA-1:cds;Parent=Msa0374410-mRNA-1
chr3_1 CDS 73871540 73871617 73871540 ID=Msa0374410-mRNA-1:cds;Parent=Msa0374410-mRNA-1
chr3_1 CDS 73870294 73870361 73870294 ID=Msa0374410-mRNA-1:cds;Parent=Msa0374410-mRNA-1
chr3_1 CDS 73869791 73870079 73869791 ID=Msa0374410-mRNA-1:cds;Parent=Msa0374410-mRNA-1
chr3_1 three_prime_UTR 73869571 73869790 73869571 ID=Msa0374410-mRNA-1:three_prime_utr;Parent=Msa0374410-mRNA-1
Gene Sequence

>Msa0374410

ATGGAGCTTGCTTTGGATAGTAGAAAATTACAATTACAACCACAAAACTTGAAGCAAGAACACTTTCATGTGTTGGCTGTAGATGATAGTGTCATTGATAGGATGCTTCTAGAGAGGCTTCTTAGAGATTCTTCATGCAAAGTTACCTGTGTGGACTCTGGAGATAAGGCTTTGAAATATCTTGGATTGAATATTGATGAAATTGATAATAATTCTTCCTCAACAAAGTCTTCTATATTAGAATCATCATCTCCTCCTCTTCAACAACCATTGCAGCTACAAGAGGGAAGCAAAGTGAATTTGATCATGACAGATTATTGCATGCCTGGGATGAGTGGCTATGATTTACTTAAAAGAGTCAAGGGATCTTCTTGGAAAGATGTTCCAGTTGTGGTTATGTCATCAGAAAATGTACCTTCTAGAATTAGCATGTGCTTGGAAGAAGGGGCAGAAGAGTTTCTAATGAAGCCTCTTCAGTTATCAGATTTGCAGAAGCTTCAACCATACTTCCTAAAATCCCTTGAAAATTCTTCTGATGAGCAAGAATCTGCCAACAGTTCCACTACCACCACAGACTCGGACGACGACAACGATCTCATCGACAGCAACAATAACAATAATGATGATAACATTAATAGTATCAGCAAACGGAAGGCCATGTCTCCTGAGCCTCCAGAGAGATCAATGCCCAAAATGAAAGGGTTGGCGGTGGTGGTGTAA
Protein sequence

>Msa0374410

MELALDSRKLQLQPQNLKQEHFHVLAVDDSVIDRMLLERLLRDSSCKVTCVDSGDKALKYLGLNIDEIDNNSSSTKSSILESSSPPLQQPLQLQEGSKVNLIMTDYCMPGMSGYDLLKRVKGSSWKDVPVVVMSSENVPSRISMCLEEGAEEFLMKPLQLSDLQKLQPYFLKSLENSSDEQESANSSTTTTDSDDDNDLIDSNNNNNDDNINSISKRKAMSPEPPERSMPKMKGLAVVV*