Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0028150 | Msa1165210 | 0.800385 | 1.477555e-48 | -8.615850e-47 |
| Msa0079560 | Msa1165210 | 0.801472 | 8.861501e-49 | -8.615850e-47 |
| Msa0086160 | Msa1165210 | 0.889372 | 2.569959e-73 | -8.615850e-47 |
| Msa0602900 | Msa1165210 | 0.827963 | 1.170004e-54 | -8.615850e-47 |
| Msa0643960 | Msa1165210 | 0.822296 | 2.556119e-53 | -8.615850e-47 |
| Msa0656450 | Msa1165210 | 0.824864 | 6.407204e-54 | -8.615850e-47 |
| Msa0663400 | Msa1165210 | 0.823613 | 1.260471e-53 | -8.615850e-47 |
| Msa0798590 | Msa1165210 | 0.846268 | 2.416290e-59 | -8.615850e-47 |
| Msa0127230 | Msa1165210 | 0.800576 | 1.350629e-48 | -8.615850e-47 |
| Msa0172300 | Msa1165210 | 0.801273 | 9.733324e-49 | -8.615850e-47 |
| Msa0182100 | Msa1165210 | 0.834281 | 3.282506e-56 | -8.615850e-47 |
| Msa0192300 | Msa1165210 | 0.806686 | 7.292135e-50 | -8.615850e-47 |
| Msa0373350 | Msa1165210 | 0.860855 | 1.543567e-63 | -8.615850e-47 |
| Msa0377370 | Msa1165210 | 0.819430 | 1.166041e-52 | -8.615850e-47 |
| Msa0383690 | Msa1165210 | 0.800921 | 1.148655e-48 | -8.615850e-47 |
| Msa0421920 | Msa1165210 | 0.827447 | 1.556825e-54 | -8.615850e-47 |
| Msa0426940 | Msa1165210 | 0.836162 | 1.099678e-56 | -8.615850e-47 |
| Msa0433060 | Msa1165210 | 0.877262 | 7.234962e-69 | -8.615850e-47 |
| Msa1013430 | Msa1165210 | 0.841424 | 4.791397e-58 | -8.615850e-47 |
| Msa1031740 | Msa1165210 | 0.833486 | 5.188142e-56 | -8.615850e-47 |
| Msa1036880 | Msa1165210 | 0.803493 | 3.395293e-49 | -8.615850e-47 |
| Msa1070810 | Msa1165210 | 0.850475 | 1.658173e-60 | -8.615850e-47 |
| Msa1082050 | Msa1165210 | 0.803488 | 3.403468e-49 | -8.615850e-47 |
| Msa1085850 | Msa1165210 | 0.872874 | 2.273359e-67 | -8.615850e-47 |
| Msa0270160 | Msa1165210 | 0.836001 | 1.208347e-56 | -8.615850e-47 |
| Msa0301250 | Msa1165210 | 0.800351 | 1.501303e-48 | -8.615850e-47 |
| Msa0313060 | Msa1165210 | 0.818032 | 2.421490e-52 | -8.615850e-47 |
| Msa1115190 | Msa1165210 | 0.844700 | 6.426289e-59 | -8.615850e-47 |
| Msa1118790 | Msa1165210 | 0.860485 | 1.999048e-63 | -8.615850e-47 |
| Msa1130680 | Msa1165210 | 0.842557 | 2.405202e-58 | -8.615850e-47 |
| Msa1136880 | Msa1165210 | 0.803915 | 2.775267e-49 | -8.615850e-47 |
| Msa1158630 | Msa1165210 | 0.847479 | 1.127150e-59 | -8.615850e-47 |
| Msa1163980 | Msa1165210 | 0.841038 | 6.055303e-58 | -8.615850e-47 |
| Msa1165210 | Msa1219760 | 0.811896 | 5.565128e-51 | -8.615850e-47 |
| Msa1165210 | Msa1235950 | 0.812250 | 4.659272e-51 | -8.615850e-47 |
| Msa1165210 | Msa1235980 | 0.804406 | 2.193238e-49 | -8.615850e-47 |
| Msa1165210 | Msa1341780 | 0.817299 | 3.543223e-52 | -8.615850e-47 |
| Msa1165210 | Msa1366540 | 0.810166 | 1.319507e-50 | -8.615850e-47 |
| Msa1165210 | Msa1403520 | 0.839578 | 1.457273e-57 | -8.615850e-47 |
| Msa1165210 | Msa1407550 | 0.838709 | 2.447915e-57 | -8.615850e-47 |
| Msa1165210 | Msa1413570 | 0.822333 | 2.505014e-53 | -8.615850e-47 |
| Msa1165210 | Msa1446510 | 0.801649 | 8.148903e-49 | -8.615850e-47 |
| Msa1165210 | Msa1462580 | 0.866565 | 2.599074e-65 | -8.615850e-47 |
| Msa0521730 | Msa1165210 | 0.846059 | 2.754637e-59 | -8.615850e-47 |
| Msa0524340 | Msa1165210 | 0.806395 | 8.399286e-50 | -8.615850e-47 |
| Msa0524680 | Msa1165210 | 0.877970 | 4.097739e-69 | -8.615850e-47 |
| Msa0547920 | Msa1165210 | 0.805424 | 1.343946e-49 | -8.615850e-47 |
| Msa0568380 | Msa1165210 | 0.800020 | 1.753268e-48 | -8.615850e-47 |
| Msa0574780 | Msa1165210 | 0.832776 | 7.795115e-56 | -8.615850e-47 |
| Msa0578010 | Msa1165210 | 0.815755 | 7.856390e-52 | -8.615850e-47 |
| Msa0585570 | Msa1165210 | 0.827788 | 1.289112e-54 | -8.615850e-47 |
| Msa0601130 | Msa1165210 | 0.809546 | 1.793634e-50 | -8.615850e-47 |
| Msa0930890 | Msa1165210 | 0.837209 | 5.949460e-57 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 39 sgRNAs with CRISPR-Local
Find 47 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAGAGCAGCTTTAGGTGTTT+AGG | 0.205838 | 7_4:-57059120 | None:intergenic |
| AGAGCAGCTTTAGGTGTTTA+GGG | 0.312485 | 7_4:-57059119 | None:intergenic |
| TGCACCCTTCTTCTTTCCCT+TGG | 0.344547 | 7_4:-57059359 | None:intergenic |
| CAAGAGATGAGAGCAGCTTT+AGG | 0.353880 | 7_4:-57059128 | None:intergenic |
| TATACGGTGGGTTTCGAGAT+TGG | 0.359508 | 7_4:-57059080 | None:intergenic |
| ATACGGTGGGTTTCGAGATT+GGG | 0.371085 | 7_4:-57059079 | None:intergenic |
| AAAACATGAAGAGTGCAAAA+AGG | 0.392063 | 7_4:+57059019 | None:intergenic |
| TCCATGATCTTGTCTTCCAC+TGG | 0.448631 | 7_4:-57059406 | None:intergenic |
| GGTGGAAAAGCTGGTGCTCT+TGG | 0.478615 | 7_4:+57059472 | Msa1165210:three_prime_UTR |
| TCTCTTGCAACCCTCGCCGC+TGG | 0.489525 | 7_4:+57059144 | Msa1165210:CDS |
| ATCAAGGTCGGTGGAAAAGC+TGG | 0.491281 | 7_4:+57059463 | Msa1165210:three_prime_UTR |
| GTTTCGAGATTGGGCTGACC+TGG | 0.507018 | 7_4:-57059070 | None:intergenic |
| ATGAGTCGTGGTAACGCAGC+TGG | 0.519123 | 7_4:+57059334 | Msa1165210:three_prime_UTR |
| CACTGGCTTAGCACAGTCAA+TGG | 0.524581 | 7_4:-57059389 | None:intergenic |
| ATGATGCATAGATTCACAAA+AGG | 0.527231 | 7_4:+57059302 | Msa1165210:intron |
| TGGTGCCAAGGGAAAGAAGA+AGG | 0.529349 | 7_4:+57059354 | Msa1165210:three_prime_UTR |
| CTTGCTCTTCTCACGGGTAA+CGG | 0.534877 | 7_4:-57059506 | None:intergenic |
| TGGTAACGCAGCTGGTGCCA+AGG | 0.535573 | 7_4:+57059342 | Msa1165210:three_prime_UTR |
| GCATAGATTCACAAAAGGTA+AGG | 0.538383 | 7_4:+57059307 | Msa1165210:intron |
| GTTTCTTCAAGAGCGAATCA+AGG | 0.539769 | 7_4:+57059447 | Msa1165210:three_prime_UTR |
| TGAAGAGAGACCAGCGGCGA+GGG | 0.543454 | 7_4:-57059154 | None:intergenic |
| AGTGATCTTGCTCTTCTCAC+GGG | 0.552636 | 7_4:-57059512 | None:intergenic |
| CAGTGATCTTGCTCTTCTCA+CGG | 0.553223 | 7_4:-57059513 | None:intergenic |
| GCCAGTGGAAGACAAGATCA+TGG | 0.553292 | 7_4:+57059405 | Msa1165210:three_prime_UTR |
| GTAAATGAATTATTACCGCT+TGG | 0.554629 | 7_4:-57059557 | None:intergenic |
| GAGAGGGAATTACCGTTCGA+AGG | 0.562138 | 7_4:-57059179 | None:intergenic |
| CTTCTCACGGGTAACGGTGA+CGG | 0.568235 | 7_4:-57059500 | None:intergenic |
| GGTAACGCAGCTGGTGCCAA+GGG | 0.575575 | 7_4:+57059343 | Msa1165210:three_prime_UTR |
| CTCCACTAAATAAAATCACC+AGG | 0.599383 | 7_4:+57059052 | Msa1165210:CDS |
| CTTAGCACAGTCAATGGTGA+AGG | 0.610280 | 7_4:-57059383 | None:intergenic |
| CTTCAAGAGCGAATCAAGGT+CGG | 0.612896 | 7_4:+57059451 | Msa1165210:three_prime_UTR |
| CTTGGAAGAGTTACTGTCAG+AGG | 0.615382 | 7_4:-57059539 | None:intergenic |
| CATTGACTGTGCTAAGCCAG+TGG | 0.621872 | 7_4:+57059390 | Msa1165210:three_prime_UTR |
| TTGAAGAGAGACCAGCGGCG+AGG | 0.633100 | 7_4:-57059155 | None:intergenic |
| CAAGAGCGAATCAAGGTCGG+TGG | 0.643278 | 7_4:+57059454 | Msa1165210:three_prime_UTR |
| GGTGCCAAGGGAAAGAAGAA+GGG | 0.657143 | 7_4:+57059355 | Msa1165210:three_prime_UTR |
| TCTCTCTTCAAACCTTCGAA+CGG | 0.668536 | 7_4:+57059167 | Msa1165210:CDS |
| AAGGTTTGAAGAGAGACCAG+CGG | 0.677612 | 7_4:-57059160 | None:intergenic |
| CTGACAGTAACTCTTCCAAG+CGG | 0.747014 | 7_4:+57059542 | Msa1165210:three_prime_UTR |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTGATATGTGATTTTATA+CGG | - | chr7_4:57059099-57059118 | None:intergenic | 15.0% |
| !!! | CATTTTCATTTTCATAAACT+CGG | + | chr7_4:57059224-57059243 | Msa1165210:intron | 20.0% |
| ! | ATCTATTAACAGAAAATCAG+TGG | - | chr7_4:57059258-57059277 | None:intergenic | 25.0% |
| ! | GATATGTGATTTTATACGGT+GGG | - | chr7_4:57059095-57059114 | None:intergenic | 30.0% |
| ! | TGATATGTGATTTTATACGG+TGG | - | chr7_4:57059096-57059115 | None:intergenic | 30.0% |
| TAATCTATTCGTACAAGAGA+GGG | - | chr7_4:57059198-57059217 | None:intergenic | 30.0% | |
| ATAATCTATTCGTACAAGAG+AGG | - | chr7_4:57059199-57059218 | None:intergenic | 30.0% | |
| ATGATGCATAGATTCACAAA+AGG | + | chr7_4:57059302-57059321 | Msa1165210:intron | 30.0% | |
| GTAAATGAATTATTACCGCT+TGG | - | chr7_4:57059560-57059579 | None:intergenic | 30.0% | |
| CTCCACTAAATAAAATCACC+AGG | + | chr7_4:57059052-57059071 | Msa1165210:CDS | 35.0% | |
| !! | GACCTGGTGATTTTATTTAG+TGG | - | chr7_4:57059057-57059076 | None:intergenic | 35.0% |
| GCATAGATTCACAAAAGGTA+AGG | + | chr7_4:57059307-57059326 | Msa1165210:intron | 35.0% | |
| !! | AGAGCAGCTTTAGGTGTTTA+GGG | - | chr7_4:57059122-57059141 | None:intergenic | 40.0% |
| TCTCTCTTCAAACCTTCGAA+CGG | + | chr7_4:57059167-57059186 | Msa1165210:CDS | 40.0% | |
| AGGTAAGGAAAAATGAGTCG+TGG | + | chr7_4:57059322-57059341 | Msa1165210:exon | 40.0% | |
| GTTTCTTCAAGAGCGAATCA+AGG | + | chr7_4:57059447-57059466 | Msa1165210:three_prime_UTR | 40.0% | |
| ! | ATACGGTGGGTTTCGAGATT+GGG | - | chr7_4:57059082-57059101 | None:intergenic | 45.0% |
| ! | TATACGGTGGGTTTCGAGAT+TGG | - | chr7_4:57059083-57059102 | None:intergenic | 45.0% |
| !! | GAGAGCAGCTTTAGGTGTTT+AGG | - | chr7_4:57059123-57059142 | None:intergenic | 45.0% |
| CAAGAGATGAGAGCAGCTTT+AGG | - | chr7_4:57059131-57059150 | None:intergenic | 45.0% | |
| AAGGTTTGAAGAGAGACCAG+CGG | - | chr7_4:57059163-57059182 | None:intergenic | 45.0% | |
| CTTAGCACAGTCAATGGTGA+AGG | - | chr7_4:57059386-57059405 | None:intergenic | 45.0% | |
| TCCATGATCTTGTCTTCCAC+TGG | - | chr7_4:57059409-57059428 | None:intergenic | 45.0% | |
| CTTCAAGAGCGAATCAAGGT+CGG | + | chr7_4:57059451-57059470 | Msa1165210:three_prime_UTR | 45.0% | |
| AGTGATCTTGCTCTTCTCAC+GGG | - | chr7_4:57059515-57059534 | None:intergenic | 45.0% | |
| CAGTGATCTTGCTCTTCTCA+CGG | - | chr7_4:57059516-57059535 | None:intergenic | 45.0% | |
| CTTGGAAGAGTTACTGTCAG+AGG | - | chr7_4:57059542-57059561 | None:intergenic | 45.0% | |
| CTGACAGTAACTCTTCCAAG+CGG | + | chr7_4:57059542-57059561 | Msa1165210:three_prime_UTR | 45.0% | |
| GAGAGGGAATTACCGTTCGA+AGG | - | chr7_4:57059182-57059201 | None:intergenic | 50.0% | |
| ! | TGGTGCCAAGGGAAAGAAGA+AGG | + | chr7_4:57059354-57059373 | Msa1165210:three_prime_UTR | 50.0% |
| GGTGCCAAGGGAAAGAAGAA+GGG | + | chr7_4:57059355-57059374 | Msa1165210:three_prime_UTR | 50.0% | |
| TGCACCCTTCTTCTTTCCCT+TGG | - | chr7_4:57059362-57059381 | None:intergenic | 50.0% | |
| !! | CACTGGCTTAGCACAGTCAA+TGG | - | chr7_4:57059392-57059411 | None:intergenic | 50.0% |
| ! | CATTGACTGTGCTAAGCCAG+TGG | + | chr7_4:57059390-57059409 | Msa1165210:three_prime_UTR | 50.0% |
| GCCAGTGGAAGACAAGATCA+TGG | + | chr7_4:57059405-57059424 | Msa1165210:three_prime_UTR | 50.0% | |
| ATCAAGGTCGGTGGAAAAGC+TGG | + | chr7_4:57059463-57059482 | Msa1165210:three_prime_UTR | 50.0% | |
| CTTGCTCTTCTCACGGGTAA+CGG | - | chr7_4:57059509-57059528 | None:intergenic | 50.0% | |
| GTTTCGAGATTGGGCTGACC+TGG | - | chr7_4:57059073-57059092 | None:intergenic | 55.0% | |
| ! | ATGAGTCGTGGTAACGCAGC+TGG | + | chr7_4:57059334-57059353 | Msa1165210:three_prime_UTR | 55.0% |
| CAAGAGCGAATCAAGGTCGG+TGG | + | chr7_4:57059454-57059473 | Msa1165210:three_prime_UTR | 55.0% | |
| GGTGGAAAAGCTGGTGCTCT+TGG | + | chr7_4:57059472-57059491 | Msa1165210:three_prime_UTR | 55.0% | |
| ! | CTTCTCACGGGTAACGGTGA+CGG | - | chr7_4:57059503-57059522 | None:intergenic | 55.0% |
| TGAAGAGAGACCAGCGGCGA+GGG | - | chr7_4:57059157-57059176 | None:intergenic | 60.0% | |
| TTGAAGAGAGACCAGCGGCG+AGG | - | chr7_4:57059158-57059177 | None:intergenic | 60.0% | |
| ! | TGGTAACGCAGCTGGTGCCA+AGG | + | chr7_4:57059342-57059361 | Msa1165210:three_prime_UTR | 60.0% |
| ! | GGTAACGCAGCTGGTGCCAA+GGG | + | chr7_4:57059343-57059362 | Msa1165210:three_prime_UTR | 60.0% |
| TCTCTTGCAACCCTCGCCGC+TGG | + | chr7_4:57059144-57059163 | Msa1165210:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_4 | gene | 57059024 | 57059592 | 57059024 | ID=Msa1165210;Name=Msa1165210 |
| chr7_4 | mRNA | 57059024 | 57059592 | 57059024 | ID=Msa1165210-mRNA-1;Parent=Msa1165210;Name=Msa1165210-mRNA-1;_AED=0.12;_eAED=0.12;_QI=0|1|0.5|1|0|0|2|265|59 |
| chr7_4 | exon | 57059024 | 57059188 | 57059024 | ID=Msa1165210-mRNA-1:exon:17118;Parent=Msa1165210-mRNA-1 |
| chr7_4 | exon | 57059313 | 57059592 | 57059313 | ID=Msa1165210-mRNA-1:exon:17119;Parent=Msa1165210-mRNA-1 |
| chr7_4 | CDS | 57059024 | 57059188 | 57059024 | ID=Msa1165210-mRNA-1:cds;Parent=Msa1165210-mRNA-1 |
| chr7_4 | CDS | 57059313 | 57059327 | 57059313 | ID=Msa1165210-mRNA-1:cds;Parent=Msa1165210-mRNA-1 |
| chr7_4 | three_prime_UTR | 57059328 | 57059592 | 57059328 | ID=Msa1165210-mRNA-1:three_prime_utr;Parent=Msa1165210-mRNA-1 |
| Gene Sequence |
| Protein sequence |
