AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0619200

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0619200 MtrunA17_Chr4g0052681 81.351 370 66 1 1 367 227 596 0.0 600
Msa0619200 MtrunA17_Chr8g0383081 77.465 355 77 2 1 355 224 575 0.0 569
Msa0619200 MtrunA17_Chr8g0383041 75.462 379 86 2 1 375 227 602 0.0 565
Msa0619200 MtrunA17_Chr8g0383061 80.088 226 41 1 129 354 1 222 1.34e-122 353
Msa0619200 MtrunA17_Chr8g0383091 91.781 146 10 1 129 274 1 144 3.24e-93 275
Msa0619200 MtrunA17_Chr8g0383071 75.524 143 35 0 1 143 168 310 1.78e-74 233
Msa0619200 MtrunA17_Chr4g0053391 40.672 268 150 3 79 346 1 259 1.73e-64 206
Msa0619200 MtrunA17_Chr8g0383211 36.950 341 205 4 2 340 314 646 1.03e-62 212
Msa0619200 MtrunA17_Chr7g0263231 30.816 331 223 3 2 329 741 1068 2.30e-47 172
Msa0619200 MtrunA17_Chr1g0192041 42.857 182 103 1 30 210 60 241 1.07e-45 157
Msa0619200 MtrunA17_Chr8g0359341 33.234 334 208 5 2 331 386 708 5.04e-45 164
Msa0619200 MtrunA17_Chr2g0308821 28.402 338 235 3 2 335 330 664 2.44e-43 159
Msa0619200 MtrunA17_Chr7g0263261 30.211 331 225 3 2 329 682 1009 3.29e-42 157
Msa0619200 MtrunA17_Chr5g0408131 34.389 221 136 4 129 347 23 236 6.68e-37 134
Msa0619200 MtrunA17_Chr8g0354631 26.781 351 228 10 2 329 358 702 6.59e-35 135
Msa0619200 MtrunA17_Chr2g0333261 28.107 338 229 7 2 330 347 679 9.07e-35 135
Msa0619200 MtrunA17_Chr4g0003151 26.269 335 237 5 2 329 328 659 9.93e-35 135
Msa0619200 MtrunA17_Chr8g0383051 68.966 87 25 2 1 85 239 325 5.12e-32 123
Msa0619200 MtrunA17_Chr5g0402891 25.824 364 251 8 2 351 243 601 6.05e-31 124
Msa0619200 MtrunA17_Chr8g0383171 30.102 196 122 4 151 341 58 243 5.09e-22 94.4
Msa0619200 MtrunA17_Chr4g0052611 28.996 269 144 9 123 369 6 249 1.16e-21 92.8
Msa0619200 MtrunA17_Chr3g0108001 43.269 104 48 3 261 362 5 99 4.57e-20 84.7
Msa0619200 MtrunA17_Chr8g0390371 41.026 78 46 0 261 338 9 86 1.17e-16 74.7
Msa0619200 MtrunA17_Chr4g0062411 23.839 323 219 7 32 348 367 668 9.50e-14 72.8
Msa0619200 MtrunA17_Chr1g0162951 50.980 51 25 0 282 332 1 51 4.13e-12 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0619200 AT3G42170.1 33.333 330 196 5 3 329 366 674 1.43e-52 186
Msa0619200 AT3G42170.2 33.333 330 196 5 3 329 344 652 1.45e-52 185
Msa0619200 AT1G18560.1 25.490 255 140 7 126 359 463 688 7.57e-11 64.3

Find 99 sgRNAs with CRISPR-Local

Find 153 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAGCAAGTTTGACAAATTT+TGG 0.118977 4_2:+70312598 Msa0619200:CDS
TCTCATCTTGGTTTGCAAAA+TGG 0.163789 4_2:+70312058 Msa0619200:five_prime_UTR
CTCATCTTGGTTTGCAAAAT+GGG 0.245980 4_2:+70312059 Msa0619200:five_prime_UTR
GAGTAGAAGGGAGAAATTTA+AGG 0.250530 4_2:+70312218 Msa0619200:CDS
ACGAAGTTGTACATGCTATT+TGG 0.252127 4_2:+70312747 Msa0619200:CDS
GATATGGTTTATGCACTTAC+TGG 0.279825 4_2:+70313605 Msa0619200:three_prime_UTR
TGGCATGCAAAGTACTATTC+AGG 0.287867 4_2:+70312797 Msa0619200:CDS
TGAGGCATTCAAAGAGTAGA+AGG 0.319331 4_2:+70312205 Msa0619200:CDS
ATGTTGGCAGTTTCACAAAA+AGG 0.324708 4_2:-70312450 None:intergenic
TTACTTAGATGAATCAAGTT+TGG 0.331942 4_2:+70313040 Msa0619200:CDS
AAAGTTAAAGATATCCTATT+AGG 0.333650 4_2:-70311797 None:intergenic
ATTTCCAGATCTATCAATCT+TGG 0.351942 4_2:+70313121 Msa0619200:CDS
ATCTAAGCTTCAACAAGAAT+TGG 0.358923 4_2:+70311766 Msa0619200:five_prime_UTR
TCTAGCCAAAGTACTTCTTC+TGG 0.359157 4_2:+70312777 Msa0619200:CDS
ATTCTAGCTTCCACATTCAT+AGG 0.364577 4_2:-70313242 None:intergenic
CTTCAATAACAAGTTGTTGA+TGG 0.370337 4_2:-70312995 None:intergenic
TACAGTGTTGTTCTTGCATT+GGG 0.376545 4_2:+70312627 Msa0619200:CDS
TGATTGAACTGTTGAATGAT+TGG 0.387676 4_2:+70311960 Msa0619200:five_prime_UTR
AATTGGCTACCATTCCTAAT+AGG 0.389770 4_2:+70311783 Msa0619200:five_prime_UTR
CAACAATTGAAATCTCATCT+TGG 0.396385 4_2:+70312046 Msa0619200:five_prime_UTR
GTAGGAGGTATAACAGCATT+TGG 0.404852 4_2:-70311698 None:intergenic
TATGTATGGCTGCTTGGATA+TGG 0.407136 4_2:+70313589 Msa0619200:three_prime_UTR
CAACAACTTGTTATTGAAGC+TGG 0.409267 4_2:+70312999 Msa0619200:CDS
GATTGAACTGTTGAATGATT+GGG 0.421976 4_2:+70311961 Msa0619200:five_prime_UTR
TAGTTCTGTTCGCTTGCATC+AGG 0.424044 4_2:+70312269 Msa0619200:CDS
CTCTCGCCGCCGTCTCCTTA+CGG 0.425085 4_2:+70311252 Msa0619200:five_prime_UTR
GTTTAGATTAAAACTATGTA+TGG 0.425819 4_2:+70313575 Msa0619200:three_prime_UTR
TTGAAAGTGCTCTTAAATAT+CGG 0.431487 4_2:+70312328 Msa0619200:CDS
GCACTGGCATGGTAGAAGAT+TGG 0.432488 4_2:-70312821 None:intergenic
TTTAAATTTGATTGTTGAAG+GGG 0.435695 4_2:+70312129 Msa0619200:five_prime_UTR
TGAAAGTCTTCACTTGTATG+AGG 0.439474 4_2:+70312359 Msa0619200:CDS
CAAGCCAAGATTGATAGATC+TGG 0.449154 4_2:-70313125 None:intergenic
ATTTAAGGAATGCATTGAAA+AGG 0.457935 4_2:+70312233 Msa0619200:CDS
GTCACTCATTTGTACATGAA+AGG 0.459961 4_2:+70311740 Msa0619200:five_prime_UTR
TGTAGTGTTTACCCTGCCAC+TGG 0.464555 4_2:+70311917 Msa0619200:five_prime_UTR
TCACTCATTTGTACATGAAA+GGG 0.472542 4_2:+70311741 Msa0619200:five_prime_UTR
TCAAAGCGAATCACGGGTTG+TGG 0.477617 4_2:+70311586 Msa0619200:five_prime_UTR
TAAAACTATGTATGGCTGCT+TGG 0.479040 4_2:+70313583 Msa0619200:three_prime_UTR
TGTGATGGAGAGTTCTTTCA+CGG 0.480748 4_2:+70312088 Msa0619200:five_prime_UTR
TAATTATGAGTTATGTCCTT+CGG 0.481954 4_2:+70312383 Msa0619200:CDS
CACATCTCACCACCAGTGGC+AGG 0.482440 4_2:-70311929 None:intergenic
CAAAATGGGTTGTTGTGTGA+TGG 0.482766 4_2:+70312073 Msa0619200:five_prime_UTR
ATCAGAAGCGACAGTGGCAA+TGG 0.483070 4_2:-70313165 None:intergenic
AAAGGAAGTTGCAACTAGTA+AGG 0.484075 4_2:+70312724 Msa0619200:CDS
GCATGCCAGAAGAAGTACTT+TGG 0.489819 4_2:-70312782 None:intergenic
AAGGAATGCATTGAAAAGGT+TGG 0.494889 4_2:+70312237 Msa0619200:CDS
CCACATTCATAGGCAGCATA+AGG 0.497176 4_2:-70313232 None:intergenic
TAAGCCTCAAGAACATTTGT+AGG 0.497271 4_2:-70311716 None:intergenic
TAGTTGCAACTTCCTTTCAC+AGG 0.501132 4_2:-70312718 None:intergenic
GAAATTATGAATGAAATTGA+CGG 0.501191 4_2:+70313432 Msa0619200:CDS
CTCTCCGATGTCTCATGTAC+AGG 0.507445 4_2:+70311278 Msa0619200:five_prime_UTR
AACTCCAAGTCTTTATCTCA+CGG 0.512124 4_2:+70312849 Msa0619200:CDS
TCAGAAGCGACAGTGGCAAT+GGG 0.512443 4_2:-70313164 None:intergenic
GGCATGCAAAGTACTATTCA+GGG 0.514294 4_2:+70312798 Msa0619200:CDS
TGAGCGGTCAAACAAATGTA+AGG 0.517830 4_2:-70311848 None:intergenic
ACGTGTTCTAGTTGATACTC+TGG 0.520472 4_2:+70312533 Msa0619200:CDS
CTAGAATTTGTACTCGCAGT+TGG 0.528677 4_2:+70313258 Msa0619200:CDS
ACCTCCTACAAATGTTCTTG+AGG 0.536836 4_2:+70311712 Msa0619200:five_prime_UTR
AGAAGAGAAAGTGAGAAATG+AGG 0.540268 4_2:-70311218 None:intergenic
ATGGTTTATGCACTTACTGG+TGG 0.549227 4_2:+70313608 Msa0619200:three_prime_UTR
TAAAGATATCCTATTAGGAA+TGG 0.555256 4_2:-70311792 None:intergenic
GGTAGATCAAAGCGAATCAC+GGG 0.561365 4_2:+70311580 Msa0619200:five_prime_UTR
AGGTAGATCAAAGCGAATCA+CGG 0.562854 4_2:+70311579 Msa0619200:five_prime_UTR
CGTTGTGTCATTAATCATGT+TGG 0.563914 4_2:-70312466 None:intergenic
ATTGAACTGTTGAATGATTG+GGG 0.569657 4_2:+70311962 Msa0619200:five_prime_UTR
CCTTATGCTGCCTATGAATG+TGG 0.570956 4_2:+70313232 Msa0619200:CDS
TGTTGTTCTTGCATTGGGAG+CGG 0.571678 4_2:+70312632 Msa0619200:CDS
GTACAGTGTTGTTCTTGCAT+TGG 0.574507 4_2:+70312626 Msa0619200:CDS
GAATTGGAAGTTAAACAGTA+AGG 0.575075 4_2:+70311883 Msa0619200:five_prime_UTR
TATGTCCTTCGGCAGAAGAG+TGG 0.576340 4_2:+70312394 Msa0619200:CDS
ACATGGATGTATTAGAATGG+TGG 0.578505 4_2:+70313081 Msa0619200:CDS
TTGATGCATCAACCTGTGAA+AGG 0.579104 4_2:+70312706 Msa0619200:CDS
TGCTTGCTAATGTTTAATGT+CGG 0.580071 4_2:+70313691 Msa0619200:three_prime_UTR
TTATGAAGAGAATGGCTGAA+AGG 0.593683 4_2:+70312571 Msa0619200:CDS
TTCACACATCTCACCACCAG+TGG 0.597954 4_2:-70311933 None:intergenic
TTAAATTTGATTGTTGAAGG+GGG 0.598852 4_2:+70312130 Msa0619200:five_prime_UTR
GCCTCAAGAACATTTGTAGG+AGG 0.599415 4_2:-70311713 None:intergenic
CTCGAAATCAGAAGCGACAG+TGG 0.599746 4_2:-70313171 None:intergenic
CGTGTTCTAGTTGATACTCT+GGG 0.603053 4_2:+70312534 Msa0619200:CDS
ATGTTGAAAATTTAATACGT+GGG 0.609160 4_2:-70312662 None:intergenic
TTTGATGATGACTGCGATGG+TGG 0.609880 4_2:+70313492 Msa0619200:CDS
CAAAGGGTAAATCAAGTGCA+AGG 0.611050 4_2:-70311628 None:intergenic
GAGAGAGCATCATGTTTGTG+AGG 0.611082 4_2:+70312187 Msa0619200:exon
AAGACATGGATGTATTAGAA+TGG 0.611743 4_2:+70313078 Msa0619200:CDS
GGAGAGTCACCGTAAGGAGA+CGG 0.613693 4_2:-70311261 None:intergenic
GTGATGGAGAGTTCTTTCAC+GGG 0.615672 4_2:+70312089 Msa0619200:five_prime_UTR
AGATGAAGTTATGAAGAGAA+TGG 0.624092 4_2:+70312563 Msa0619200:CDS
GACATCGGAGAGTCACCGTA+AGG 0.624904 4_2:-70311267 None:intergenic
AATGTTGAAAATTTAATACG+TGG 0.625350 4_2:-70312663 None:intergenic
AGTGTTTACCCTGCCACTGG+TGG 0.629471 4_2:+70311920 Msa0619200:five_prime_UTR
ACATCTCACCACCAGTGGCA+GGG 0.637351 4_2:-70311928 None:intergenic
AAGTGAGAAATGAGGTACGC+GGG 0.639129 4_2:-70311210 None:intergenic
GAGGCATTCAAAGAGTAGAA+GGG 0.646358 4_2:+70312206 Msa0619200:CDS
TAAACCGTGAGATAAAGACT+TGG 0.651980 4_2:-70312853 None:intergenic
AATACCTGTACATGAGACAT+CGG 0.655981 4_2:-70311282 None:intergenic
TGGAGCACGAGGGAATGAGG+AGG 0.698468 4_2:+70311657 Msa0619200:five_prime_UTR
ATTCGCGTCAACAAAATGAG+CGG 0.717347 4_2:-70311864 None:intergenic
GAGTCACCGTAAGGAGACGG+CGG 0.730294 4_2:-70311258 None:intergenic
AAAGTGAGAAATGAGGTACG+CGG 0.757437 4_2:-70311211 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATTATACCTATATAATTT+AGG + chr4_2:70311405-70311424 Msa0619200:intron 10.0%
!! TATTATACCTATATAATTTA+GGG + chr4_2:70311406-70311425 Msa0619200:intron 10.0%
!!! TGTAAGTTTATATATATTTT+TGG + chr4_2:70312881-70312900 Msa0619200:intron 10.0%
!! ATTATACCTATATAATTTAG+GGG + chr4_2:70311407-70311426 Msa0619200:intron 15.0%
!!! TATCTTTAACTTTTGATTTA+TGG + chr4_2:70311807-70311826 Msa0619200:five_prime_UTR 15.0%
!!! ATCTTTAACTTTTGATTTAT+GGG + chr4_2:70311808-70311827 Msa0619200:five_prime_UTR 15.0%
!!! TTTTAAATTTGATTGTTGAA+GGG + chr4_2:70312128-70312147 Msa0619200:five_prime_UTR 15.0%
!!! TTTAGTTTATGATGATTTTT+TGG + chr4_2:70313545-70313564 Msa0619200:three_prime_UTR 15.0%
!!! TGTTTTTAATTTTTATTGTG+TGG + chr4_2:70313661-70313680 Msa0619200:three_prime_UTR 15.0%
!! GATAAGAACCTTATAAAAAA+AGG - chr4_2:70311320-70311339 None:intergenic 20.0%
!! AAAGTTAAAGATATCCTATT+AGG - chr4_2:70311800-70311819 None:intergenic 20.0%
!!! GTTTTAAATTTGATTGTTGA+AGG + chr4_2:70312127-70312146 Msa0619200:five_prime_UTR 20.0%
!! TTTAAATTTGATTGTTGAAG+GGG + chr4_2:70312129-70312148 Msa0619200:five_prime_UTR 20.0%
!! AAAAAATACAAATTGGAAGT+AGG - chr4_2:70312495-70312514 None:intergenic 20.0%
!! AACTTGCAAAAAATACAAAT+TGG - chr4_2:70312502-70312521 None:intergenic 20.0%
!!! ATTTGTATTTTTTGCAAGTT+TGG + chr4_2:70312502-70312521 Msa0619200:CDS 20.0%
!! ATGTTGAAAATTTAATACGT+GGG - chr4_2:70312665-70312684 None:intergenic 20.0%
!! AATGTTGAAAATTTAATACG+TGG - chr4_2:70312666-70312685 None:intergenic 20.0%
!!! ACGTATTAAATTTTCAACAT+TGG + chr4_2:70312665-70312684 Msa0619200:CDS 20.0%
!! TTAAACAAATTCAAGATAGA+AGG - chr4_2:70312924-70312943 None:intergenic 20.0%
!! GAAATTATGAATGAAATTGA+CGG + chr4_2:70313432-70313451 Msa0619200:CDS 20.0%
!! GTTTAGATTAAAACTATGTA+TGG + chr4_2:70313575-70313594 Msa0619200:three_prime_UTR 20.0%
!!! TCAAACTGCCTTTTTTTATA+AGG + chr4_2:70311309-70311328 Msa0619200:intron 25.0%
! ATAAAATCCGTCAAAATTGA+CGG - chr4_2:70311369-70311388 None:intergenic 25.0%
!!! TGACGGATTTTATATACTTT+AGG + chr4_2:70311376-70311395 Msa0619200:intron 25.0%
!!! GACGGATTTTATATACTTTA+GGG + chr4_2:70311377-70311396 Msa0619200:intron 25.0%
! TTTAGTCCCCTAAATTATAT+AGG - chr4_2:70311416-70311435 None:intergenic 25.0%
! ATAATGCTGAAATGTAATCT+AGG + chr4_2:70311514-70311533 Msa0619200:intron 25.0%
!!! TGATTTACCCTTTGATTTTT+TGG + chr4_2:70311637-70311656 Msa0619200:five_prime_UTR 25.0%
! TAAAGATATCCTATTAGGAA+TGG - chr4_2:70311795-70311814 None:intergenic 25.0%
! TTAAATTTGATTGTTGAAGG+GGG + chr4_2:70312130-70312149 Msa0619200:five_prime_UTR 25.0%
! ATTTAAGGAATGCATTGAAA+AGG + chr4_2:70312233-70312252 Msa0619200:CDS 25.0%
!!! TTGAAAGTGCTCTTAAATAT+CGG + chr4_2:70312328-70312347 Msa0619200:CDS 25.0%
! TAATTATGAGTTATGTCCTT+CGG + chr4_2:70312383-70312402 Msa0619200:CDS 25.0%
! ATTACATTTCACAAGTGAAA+AGG - chr4_2:70312959-70312978 None:intergenic 25.0%
! TTTCACTTGTGAAATGTAAT+AGG + chr4_2:70312959-70312978 Msa0619200:intron 25.0%
! TTACTTAGATGAATCAAGTT+TGG + chr4_2:70313040-70313059 Msa0619200:CDS 25.0%
! TCTACATATCAAAATTGAAG+AGG - chr4_2:70313384-70313403 None:intergenic 25.0%
!!! ATTTTGATATGTAGATGAAG+AGG + chr4_2:70313389-70313408 Msa0619200:intron 25.0%
!!! ATGATGATTTTTTGGCTTTA+TGG + chr4_2:70313553-70313572 Msa0619200:three_prime_UTR 25.0%
! TGAAAATCCGTCAATTTTGA+CGG + chr4_2:70311359-70311378 Msa0619200:intron 30.0%
TGATAGTTTAGAGACTATTC+TGG + chr4_2:70311448-70311467 Msa0619200:intron 30.0%
AGTCATGTCACAAGAAAAAA+TGG + chr4_2:70311544-70311563 Msa0619200:five_prime_UTR 30.0%
AAAAATGGAATTTGACGATG+AGG + chr4_2:70311559-70311578 Msa0619200:five_prime_UTR 30.0%
TCACTCATTTGTACATGAAA+GGG + chr4_2:70311741-70311760 Msa0619200:five_prime_UTR 30.0%
ATCTAAGCTTCAACAAGAAT+TGG + chr4_2:70311766-70311785 Msa0619200:five_prime_UTR 30.0%
GAATTGGAAGTTAAACAGTA+AGG + chr4_2:70311883-70311902 Msa0619200:five_prime_UTR 30.0%
TGATTGAACTGTTGAATGAT+TGG + chr4_2:70311960-70311979 Msa0619200:five_prime_UTR 30.0%
GATTGAACTGTTGAATGATT+GGG + chr4_2:70311961-70311980 Msa0619200:five_prime_UTR 30.0%
ATTGAACTGTTGAATGATTG+GGG + chr4_2:70311962-70311981 Msa0619200:five_prime_UTR 30.0%
CAACAATTGAAATCTCATCT+TGG + chr4_2:70312046-70312065 Msa0619200:five_prime_UTR 30.0%
AGATGAAGTTATGAAGAGAA+TGG + chr4_2:70312563-70312582 Msa0619200:CDS 30.0%
! TGAGCAAGTTTGACAAATTT+TGG + chr4_2:70312598-70312617 Msa0619200:CDS 30.0%
CTTCAATAACAAGTTGTTGA+TGG - chr4_2:70312998-70313017 None:intergenic 30.0%
! TTTTCGTTGTTATGAAGACA+TGG + chr4_2:70313064-70313083 Msa0619200:CDS 30.0%
AAGACATGGATGTATTAGAA+TGG + chr4_2:70313078-70313097 Msa0619200:CDS 30.0%
ATTTCCAGATCTATCAATCT+TGG + chr4_2:70313121-70313140 Msa0619200:CDS 30.0%
! TTGTACAATTTTGTCAGCAA+TGG + chr4_2:70313281-70313300 Msa0619200:CDS 30.0%
TTAATAATTGAATAGACCCG+TGG - chr4_2:70313361-70313380 None:intergenic 30.0%
AGAAGAGAAAGTGAGAAATG+AGG - chr4_2:70311221-70311240 None:intergenic 35.0%
AATACCTGTACATGAGACAT+CGG - chr4_2:70311285-70311304 None:intergenic 35.0%
CTGAAATGTAATCTAGGTTG+TGG + chr4_2:70311520-70311539 Msa0619200:intron 35.0%
TCGTGCTCCAAAAAATCAAA+GGG - chr4_2:70311647-70311666 None:intergenic 35.0%
!!! TTTGATTTTTTGGAGCACGA+GGG + chr4_2:70311647-70311666 Msa0619200:five_prime_UTR 35.0%
TAAGCCTCAAGAACATTTGT+AGG - chr4_2:70311719-70311738 None:intergenic 35.0%
GTCACTCATTTGTACATGAA+AGG + chr4_2:70311740-70311759 Msa0619200:five_prime_UTR 35.0%
AATTGGCTACCATTCCTAAT+AGG + chr4_2:70311783-70311802 Msa0619200:five_prime_UTR 35.0%
!! TCTCATCTTGGTTTGCAAAA+TGG + chr4_2:70312058-70312077 Msa0619200:five_prime_UTR 35.0%
!! CTCATCTTGGTTTGCAAAAT+GGG + chr4_2:70312059-70312078 Msa0619200:five_prime_UTR 35.0%
! GCTCTCTCTAATTTTAGACA+CGG - chr4_2:70312175-70312194 None:intergenic 35.0%
GAGTAGAAGGGAGAAATTTA+AGG + chr4_2:70312218-70312237 Msa0619200:CDS 35.0%
AAGGAATGCATTGAAAAGGT+TGG + chr4_2:70312237-70312256 Msa0619200:CDS 35.0%
TGAAAGTCTTCACTTGTATG+AGG + chr4_2:70312359-70312378 Msa0619200:CDS 35.0%
ATGTTGGCAGTTTCACAAAA+AGG - chr4_2:70312453-70312472 None:intergenic 35.0%
! CGTTGTGTCATTAATCATGT+TGG - chr4_2:70312469-70312488 None:intergenic 35.0%
TTATGAAGAGAATGGCTGAA+AGG + chr4_2:70312571-70312590 Msa0619200:CDS 35.0%
! TACAGTGTTGTTCTTGCATT+GGG + chr4_2:70312627-70312646 Msa0619200:CDS 35.0%
AAAGGAAGTTGCAACTAGTA+AGG + chr4_2:70312724-70312743 Msa0619200:CDS 35.0%
ACGAAGTTGTACATGCTATT+TGG + chr4_2:70312747-70312766 Msa0619200:CDS 35.0%
AACTCCAAGTCTTTATCTCA+CGG + chr4_2:70312849-70312868 Msa0619200:CDS 35.0%
TAAACCGTGAGATAAAGACT+TGG - chr4_2:70312856-70312875 None:intergenic 35.0%
AAGTGAAAAGGAAAGCAATG+AGG - chr4_2:70312947-70312966 None:intergenic 35.0%
!! CAACAACTTGTTATTGAAGC+TGG + chr4_2:70312999-70313018 Msa0619200:CDS 35.0%
ACATGGATGTATTAGAATGG+TGG + chr4_2:70313081-70313100 Msa0619200:CDS 35.0%
!!! TTCTGATTTCGAGTTTTGCA+TGG + chr4_2:70313181-70313200 Msa0619200:CDS 35.0%
!!! TCTGATTTCGAGTTTTGCAT+GGG + chr4_2:70313182-70313201 Msa0619200:CDS 35.0%
!! ATTCTAGCTTCCACATTCAT+AGG - chr4_2:70313245-70313264 None:intergenic 35.0%
CAAAACAACACGATTAGACA+TGG - chr4_2:70313334-70313353 None:intergenic 35.0%
!!! TCGTGTTGTTTTGATAACCA+CGG + chr4_2:70313341-70313360 Msa0619200:intron 35.0%
AATTGACGGCGATGAAAAAA+TGG + chr4_2:70313446-70313465 Msa0619200:CDS 35.0%
TAAAACTATGTATGGCTGCT+TGG + chr4_2:70313583-70313602 Msa0619200:three_prime_UTR 35.0%
GATATGGTTTATGCACTTAC+TGG + chr4_2:70313605-70313624 Msa0619200:three_prime_UTR 35.0%
AAAGTGAGAAATGAGGTACG+CGG - chr4_2:70311214-70311233 None:intergenic 40.0%
TTTAGGGGACTAAAATGACG+AGG + chr4_2:70311422-70311441 Msa0619200:intron 40.0%
AGGTAGATCAAAGCGAATCA+CGG + chr4_2:70311579-70311598 Msa0619200:five_prime_UTR 40.0%
CAAAGGGTAAATCAAGTGCA+AGG - chr4_2:70311631-70311650 None:intergenic 40.0%
CTCGTGCTCCAAAAAATCAA+AGG - chr4_2:70311648-70311667 None:intergenic 40.0%
!!! CTTTGATTTTTTGGAGCACG+AGG + chr4_2:70311646-70311665 Msa0619200:five_prime_UTR 40.0%
! GTAGGAGGTATAACAGCATT+TGG - chr4_2:70311701-70311720 None:intergenic 40.0%
ACCTCCTACAAATGTTCTTG+AGG + chr4_2:70311712-70311731 Msa0619200:five_prime_UTR 40.0%
TGAGCGGTCAAACAAATGTA+AGG - chr4_2:70311851-70311870 None:intergenic 40.0%
ATTCGCGTCAACAAAATGAG+CGG - chr4_2:70311867-70311886 None:intergenic 40.0%
! CTCATTTTGTTGACGCGAAT+TGG + chr4_2:70311867-70311886 Msa0619200:five_prime_UTR 40.0%
!! CAAAATGGGTTGTTGTGTGA+TGG + chr4_2:70312073-70312092 Msa0619200:five_prime_UTR 40.0%
! TGTGATGGAGAGTTCTTTCA+CGG + chr4_2:70312088-70312107 Msa0619200:five_prime_UTR 40.0%
! TGAGGCATTCAAAGAGTAGA+AGG + chr4_2:70312205-70312224 Msa0619200:CDS 40.0%
! GAGGCATTCAAAGAGTAGAA+GGG + chr4_2:70312206-70312225 Msa0619200:CDS 40.0%
! ACGTGTTCTAGTTGATACTC+TGG + chr4_2:70312533-70312552 Msa0619200:CDS 40.0%
! CGTGTTCTAGTTGATACTCT+GGG + chr4_2:70312534-70312553 Msa0619200:CDS 40.0%
! GTACAGTGTTGTTCTTGCAT+TGG + chr4_2:70312626-70312645 Msa0619200:CDS 40.0%
TTGATGCATCAACCTGTGAA+AGG + chr4_2:70312706-70312725 Msa0619200:CDS 40.0%
TAGTTGCAACTTCCTTTCAC+AGG - chr4_2:70312721-70312740 None:intergenic 40.0%
! TCTAGCCAAAGTACTTCTTC+TGG + chr4_2:70312777-70312796 Msa0619200:CDS 40.0%
! TGGCATGCAAAGTACTATTC+AGG + chr4_2:70312797-70312816 Msa0619200:CDS 40.0%
! GGCATGCAAAGTACTATTCA+GGG + chr4_2:70312798-70312817 Msa0619200:CDS 40.0%
CAAGCCAAGATTGATAGATC+TGG - chr4_2:70313128-70313147 None:intergenic 40.0%
CTAGAATTTGTACTCGCAGT+TGG + chr4_2:70313258-70313277 Msa0619200:CDS 40.0%
!!! CGTGTTGTTTTGATAACCAC+GGG + chr4_2:70313342-70313361 Msa0619200:intron 40.0%
!!! GATTTTGATGATGACTGCGA+TGG + chr4_2:70313489-70313508 Msa0619200:CDS 40.0%
TATGTATGGCTGCTTGGATA+TGG + chr4_2:70313589-70313608 Msa0619200:three_prime_UTR 40.0%
ATGGTTTATGCACTTACTGG+TGG + chr4_2:70313608-70313627 Msa0619200:three_prime_UTR 40.0%
AAGTGAGAAATGAGGTACGC+GGG - chr4_2:70311213-70311232 None:intergenic 45.0%
GGTAGATCAAAGCGAATCAC+GGG + chr4_2:70311580-70311599 Msa0619200:five_prime_UTR 45.0%
GCCTCAAGAACATTTGTAGG+AGG - chr4_2:70311716-70311735 None:intergenic 45.0%
! GTGATGGAGAGTTCTTTCAC+GGG + chr4_2:70312089-70312108 Msa0619200:five_prime_UTR 45.0%
!! GAGAGAGCATCATGTTTGTG+AGG + chr4_2:70312187-70312206 Msa0619200:exon 45.0%
! TAGTTCTGTTCGCTTGCATC+AGG + chr4_2:70312269-70312288 Msa0619200:CDS 45.0%
TGTTGTTCTTGCATTGGGAG+CGG + chr4_2:70312632-70312651 Msa0619200:CDS 45.0%
!! GCATGCCAGAAGAAGTACTT+TGG - chr4_2:70312785-70312804 None:intergenic 45.0%
! GACTTGGAGTTCTTTTGCAC+TGG - chr4_2:70312840-70312859 None:intergenic 45.0%
CCACATTCATAGGCAGCATA+AGG - chr4_2:70313235-70313254 None:intergenic 45.0%
CCTTATGCTGCCTATGAATG+TGG + chr4_2:70313232-70313251 Msa0619200:CDS 45.0%
!! TTTGATGATGACTGCGATGG+TGG + chr4_2:70313492-70313511 Msa0619200:CDS 45.0%
CTCTCCGATGTCTCATGTAC+AGG + chr4_2:70311278-70311297 Msa0619200:five_prime_UTR 50.0%
! TCAAAGCGAATCACGGGTTG+TGG + chr4_2:70311586-70311605 Msa0619200:five_prime_UTR 50.0%
!!! TTTTGGAGCACGAGGGAATG+AGG + chr4_2:70311654-70311673 Msa0619200:five_prime_UTR 50.0%
TGTAGTGTTTACCCTGCCAC+TGG + chr4_2:70311917-70311936 Msa0619200:five_prime_UTR 50.0%
TTCACACATCTCACCACCAG+TGG - chr4_2:70311936-70311955 None:intergenic 50.0%
TATGTCCTTCGGCAGAAGAG+TGG + chr4_2:70312394-70312413 Msa0619200:CDS 50.0%
! CTTTTCCACTCTTCTGCCGA+AGG - chr4_2:70312402-70312421 None:intergenic 50.0%
! GCACTGGCATGGTAGAAGAT+TGG - chr4_2:70312824-70312843 None:intergenic 50.0%
!! GGAGTTCTTTTGCACTGGCA+TGG - chr4_2:70312835-70312854 None:intergenic 50.0%
TCAGAAGCGACAGTGGCAAT+GGG - chr4_2:70313167-70313186 None:intergenic 50.0%
ATCAGAAGCGACAGTGGCAA+TGG - chr4_2:70313168-70313187 None:intergenic 50.0%
CTCGAAATCAGAAGCGACAG+TGG - chr4_2:70313174-70313193 None:intergenic 50.0%
GGAGAGTCACCGTAAGGAGA+CGG - chr4_2:70311264-70311283 None:intergenic 55.0%
GACATCGGAGAGTCACCGTA+AGG - chr4_2:70311270-70311289 None:intergenic 55.0%
AGTGTTTACCCTGCCACTGG+TGG + chr4_2:70311920-70311939 Msa0619200:five_prime_UTR 55.0%
ACATCTCACCACCAGTGGCA+GGG - chr4_2:70311931-70311950 None:intergenic 55.0%
GAGTCACCGTAAGGAGACGG+CGG - chr4_2:70311261-70311280 None:intergenic 60.0%
TGGAGCACGAGGGAATGAGG+AGG + chr4_2:70311657-70311676 Msa0619200:five_prime_UTR 60.0%
CACATCTCACCACCAGTGGC+AGG - chr4_2:70311932-70311951 None:intergenic 60.0%
CTCTCGCCGCCGTCTCCTTA+CGG + chr4_2:70311252-70311271 Msa0619200:five_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 70311192 70313713 70311192 ID=Msa0619200;Name=Msa0619200
chr4_2 mRNA 70311192 70313713 70311192 ID=Msa0619200-mRNA-1;Parent=Msa0619200;Name=Msa0619200-mRNA-1;_AED=0.00;_eAED=0.00;_QI=770|1|1|1|0.66|0.5|4|189|375
chr4_2 exon 70311192 70311299 70311192 ID=Msa0619200-mRNA-1:exon:20451;Parent=Msa0619200-mRNA-1
chr4_2 exon 70311536 70312881 70311536 ID=Msa0619200-mRNA-1:exon:20452;Parent=Msa0619200-mRNA-1
chr4_2 exon 70312981 70313302 70312981 ID=Msa0619200-mRNA-1:exon:20453;Parent=Msa0619200-mRNA-1
chr4_2 exon 70313403 70313713 70313403 ID=Msa0619200-mRNA-1:exon:20454;Parent=Msa0619200-mRNA-1
chr4_2 five_prime_UTR 70311192 70311299 70311192 ID=Msa0619200-mRNA-1:five_prime_utr;Parent=Msa0619200-mRNA-1
chr4_2 five_prime_UTR 70311536 70312197 70311536 ID=Msa0619200-mRNA-1:five_prime_utr;Parent=Msa0619200-mRNA-1
chr4_2 CDS 70312198 70312881 70312198 ID=Msa0619200-mRNA-1:cds;Parent=Msa0619200-mRNA-1
chr4_2 CDS 70312981 70313302 70312981 ID=Msa0619200-mRNA-1:cds;Parent=Msa0619200-mRNA-1
chr4_2 CDS 70313403 70313524 70313403 ID=Msa0619200-mRNA-1:cds;Parent=Msa0619200-mRNA-1
chr4_2 three_prime_UTR 70313525 70313713 70313525 ID=Msa0619200-mRNA-1:three_prime_utr;Parent=Msa0619200-mRNA-1
Gene Sequence

>Msa0619200

ATGTTTGTGAGGCATTCAAAGAGTAGAAGGGAGAAATTTAAGGAATGCATTGAAAAGGTTGGTGCTGTTGATAGTTCTGTTCGCTTGCATCAGGACTCGTCTATGAGTCTGAACTCAACTTATTTGATGCTTGAAAGTGCTCTTAAATATCGGCGTGTGTTTGAAAGTCTTCACTTGTATGAGGATAATTATGAGTTATGTCCTTCGGCAGAAGAGTGGAAAAGAGTAGAAAAGATTTGTGCATTCTTGTTGCCTTTTTGTGAAACTGCCAACATGATTAATGACACAACGCATCCTACTTCCAATTTGTATTTTTTGCAAGTTTGGAAAGTTGAACGTGTTCTAGTTGATACTCTGGGAGATGAAGATGAAGTTATGAAGAGAATGGCTGAAAGGATGATGAGCAAGTTTGACAAATTTTGGAATGAGTACAGTGTTGTTCTTGCATTGGGAGCGGTTCTTGACCCACGTATTAAATTTTCAACATTGGCTTATTGTTACTCAAAACTTGATGCATCAACCTGTGAAAGGAAGTTGCAACTAGTAAGGACGAAGTTGTACATGCTATTTGGCAAGTATTCTAGCCAAAGTACTTCTTCTGGCATGCAAAGTACTATTCAGGGCCAATCTTCTACCATGCCAGTGCAAAAGAACTCCAAGTCTTTATCTCACGGTTTATTCGATGAATTGAAAATGCACCATCAACAACTTGTTATTGAAGCTGGAAAATCTCAACTTGATGTTTACTTAGATGAATCAAGTTTGGATTTTCGTTGTTATGAAGACATGGATGTATTAGAATGGTGGAAGAGTAATAATAATCGATTTCCAGATCTATCAATCTTGGCTTGTGACTTGTTAAGTGTTCCCATTGCCACTGTCGCTTCTGATTTCGAGTTTTGCATGGGCGCACGCGTCTTTGAAAAGTATAAAGACCTTATGCTGCCTATGAATGTGGAAGCTAGAATTTGTACTCGCAGTTGGTTGTACAATTTTGTCAGCAATGATGAAGAGGACGATGATGATGACTTTGAAGAAATTATGAATGAAATTGACGGCGATGAAAAAATGGAGAAAGCATCAAATGATTTTGATTTTGATGATGACTGCGATGGTGGTGATGATTGA
Protein sequence

>Msa0619200

MFVRHSKSRREKFKECIEKVGAVDSSVRLHQDSSMSLNSTYLMLESALKYRRVFESLHLYEDNYELCPSAEEWKRVEKICAFLLPFCETANMINDTTHPTSNLYFLQVWKVERVLVDTLGDEDEVMKRMAERMMSKFDKFWNEYSVVLALGAVLDPRIKFSTLAYCYSKLDASTCERKLQLVRTKLYMLFGKYSSQSTSSGMQSTIQGQSSTMPVQKNSKSLSHGLFDELKMHHQQLVIEAGKSQLDVYLDESSLDFRCYEDMDVLEWWKSNNNRFPDLSILACDLLSVPIATVASDFEFCMGARVFEKYKDLMLPMNVEARICTRSWLYNFVSNDEEDDDDDFEEIMNEIDGDEKMEKASNDFDFDDDCDGGDD*