AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0625140


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0625140 MtrunA17_Chr4g0000551 85.915 71 10 0 8 78 1 71 1.73e-41 130
Msa0625140 MtrunA17_Chr5g0417121 56.944 72 28 2 1 72 1 69 2.55e-22 81.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0625140 AT1G51650.1 50.000 72 33 2 1 72 1 69 1.74e-19 74.7

Find 26 sgRNAs with CRISPR-Local

Find 150 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTCAAATTCTCTCTCTTTC+TGG 0.136195 4_3:+4135373 Msa0625140:CDS
GTGGCAGATGCCCTGAATTT+TGG 0.222704 4_3:+4137718 Msa0625140:three_prime_UTR
ACTTGACAGCCAATCTTATT+AGG 0.245215 4_3:+4135326 Msa0625140:CDS
ACCATGCAAATCCAAAATTC+AGG 0.300935 4_3:-4137729 None:intergenic
ACATGGATCGACAGTATTTC+CGG 0.360913 4_3:+4135421 Msa0625140:CDS
CGACGTCGTCTTGATTCCTC+TGG 0.371709 4_3:-4137584 None:intergenic
GCAGAGGAAAAGTAGCGATT+TGG 0.379656 4_3:+4135269 Msa0625140:CDS
AAGGCAATTCCTAATAAGAT+TGG 0.392366 4_3:-4135335 None:intergenic
TCTTATTAGGAATTGCCTTA+AGG 0.405336 4_3:+4135339 Msa0625140:CDS
CTCTGGGATGTCTGTATGAA+TGG 0.437480 4_3:-4137567 None:intergenic
GTAGCGATTTGGAGAGCAGC+AGG 0.448954 4_3:+4135280 Msa0625140:CDS
GAAGCTAATCTGAGATCTGC+TGG 0.451980 4_3:+4137626 Msa0625140:three_prime_UTR
AATAAGATTGGCTGTCAAGT+TGG 0.465236 4_3:-4135323 None:intergenic
GTAACGAAAACAAGTGTAAG+AGG 0.479461 4_3:+4135194 Msa0625140:five_prime_UTR
CGATCAATGGCGTCAGGCAG+AGG 0.495109 4_3:+4135253 Msa0625140:exon
GATAATCGATCAATGGCGTC+AGG 0.508621 4_3:+4135247 Msa0625140:exon
CGTATCAGATAATCGATCAA+TGG 0.522049 4_3:+4135240 Msa0625140:exon
ATACATCTAAAACTCTAGAA+AGG 0.525234 4_3:-4137669 None:intergenic
TCGCTGAAACGCATTCATGA+TGG 0.530943 4_3:+4137604 Msa0625140:exon
CCATGCAAATCCAAAATTCA+GGG 0.535223 4_3:-4137728 None:intergenic
TTCAGTACTTGCTTCCCACA+TGG 0.547985 4_3:+4135404 Msa0625140:CDS
GACGTCGTCTTGATTCCTCT+GGG 0.578095 4_3:-4137583 None:intergenic
GAAATACTGTCGATCCATGT+GGG 0.608685 4_3:-4135418 None:intergenic
TAGCGATTTGGAGAGCAGCA+GGG 0.642009 4_3:+4135281 Msa0625140:CDS
GGAAATACTGTCGATCCATG+TGG 0.645523 4_3:-4135419 None:intergenic
CATTCATACAGACATCCCAG+AGG 0.796801 4_3:+4137568 Msa0625140:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTGAATGTATTTTTTAAA+GGG - chr4_3:4137045-4137064 None:intergenic 10.0%
!!! TTCATTTTCTTAAGTTATTA+CGG + chr4_3:4135909-4135928 Msa0625140:intron 15.0%
!!! ATTGTTTTCATTATTATTAG+TGG + chr4_3:4136189-4136208 Msa0625140:intron 15.0%
!!! ATTTTTCTTTGGAAAATAAT+TGG + chr4_3:4136925-4136944 Msa0625140:intron 15.0%
!!! CATTTGAATGTATTTTTTAA+AGG - chr4_3:4137046-4137065 None:intergenic 15.0%
!! TATACAAAACTAAATAAACT+TGG - chr4_3:4137501-4137520 None:intergenic 15.0%
!!! TTTTTTTCTTTCTAAGAACT+CGG - chr4_3:4135587-4135606 None:intergenic 20.0%
!! CCTATCATCTTAATTAATTA+GGG - chr4_3:4135777-4135796 None:intergenic 20.0%
!! CCTAATTAATTAAGATGATA+GGG + chr4_3:4135775-4135794 Msa0625140:intron 20.0%
!!! TCATTTTCTTAAGTTATTAC+GGG + chr4_3:4135910-4135929 Msa0625140:intron 20.0%
!!! ATTTTGTCTCAAAAATATCA+TGG - chr4_3:4136325-4136344 None:intergenic 20.0%
!!! CAATTTAGTCTAAAATATCA+TGG - chr4_3:4136445-4136464 None:intergenic 20.0%
!!! ATATTTTAGACTAAATTGAG+AGG + chr4_3:4136447-4136466 Msa0625140:intron 20.0%
!! TCAATTAAACAGAAATGAAA+TGG - chr4_3:4136685-4136704 None:intergenic 20.0%
!! TTCATTTCTGTTTAATTGAT+TGG + chr4_3:4136686-4136705 Msa0625140:intron 20.0%
!!! TTTTGGTAAAGATTTTTCTT+TGG + chr4_3:4136914-4136933 Msa0625140:intron 20.0%
!!! AGAATTTTATCGCTTTTAAT+CGG - chr4_3:4136972-4136991 None:intergenic 20.0%
!! ATTTCACTTTGAATCATAAT+TGG + chr4_3:4137089-4137108 Msa0625140:intron 20.0%
!!! TTTAATGTGATGTTTTCATT+AGG + chr4_3:4137191-4137210 Msa0625140:intron 20.0%
!! ATCATAACCAAAAAAGAAAA+CGG - chr4_3:4137696-4137715 None:intergenic 20.0%
! CCATAATGTTAACTTTCTTA+GGG + chr4_3:4135682-4135701 Msa0625140:intron 25.0%
! CCCTAATTAATTAAGATGAT+AGG + chr4_3:4135774-4135793 Msa0625140:intron 25.0%
! CCCTATCATCTTAATTAATT+AGG - chr4_3:4135778-4135797 None:intergenic 25.0%
!!! GAACACATTTTTTAGATTGA+AGG + chr4_3:4135815-4135834 Msa0625140:intron 25.0%
! AGATGATTTAGAAACTACTT+TGG + chr4_3:4136005-4136024 Msa0625140:intron 25.0%
!!! CATTTTCATTGATTTCTGAA+AGG - chr4_3:4136161-4136180 None:intergenic 25.0%
! TCATTTAATTACCTAACTTG+TGG + chr4_3:4136520-4136539 Msa0625140:intron 25.0%
! TTTGTGCTTTATGAAGAAAA+GGG - chr4_3:4136595-4136614 None:intergenic 25.0%
!! TTTTGTGCTTTATGAAGAAA+AGG - chr4_3:4136596-4136615 None:intergenic 25.0%
!!! CTTTTTCAAACTTTTGTCAT+TGG + chr4_3:4136728-4136747 Msa0625140:intron 25.0%
!!! CAATGAGTTTTGCTTTAAAT+TGG + chr4_3:4136992-4137011 Msa0625140:intron 25.0%
! TATTGATCTTAATCAGTTTG+AGG - chr4_3:4137128-4137147 None:intergenic 25.0%
! ATGAAAACATCACATTAAAG+TGG - chr4_3:4137190-4137209 None:intergenic 25.0%
!! ATACATCTAAAACTCTAGAA+AGG - chr4_3:4137672-4137691 None:intergenic 25.0%
!! ATGTATTCCGTTTTCTTTTT+TGG + chr4_3:4137686-4137705 Msa0625140:three_prime_UTR 25.0%
AAGGCAATTCCTAATAAGAT+TGG - chr4_3:4135338-4135357 None:intergenic 30.0%
TCTTATTAGGAATTGCCTTA+AGG + chr4_3:4135339-4135358 Msa0625140:CDS 30.0%
AATAGAAGAAGAGAGAAAGA+GGG - chr4_3:4135463-4135482 None:intergenic 30.0%
AAATAGAAGAAGAGAGAAAG+AGG - chr4_3:4135464-4135483 None:intergenic 30.0%
GCCATAATGTTAACTTTCTT+AGG + chr4_3:4135681-4135700 Msa0625140:intron 30.0%
CCCTAAGAAAGTTAACATTA+TGG - chr4_3:4135685-4135704 None:intergenic 30.0%
! CAAGCTTTGTAGAAACAAAT+GGG - chr4_3:4135722-4135741 None:intergenic 30.0%
! TCAAGCTTTGTAGAAACAAA+TGG - chr4_3:4135723-4135742 None:intergenic 30.0%
!! ATTTGTTTCTACAAAGCTTG+AGG + chr4_3:4135722-4135741 Msa0625140:intron 30.0%
!! TTTGTTTCTACAAAGCTTGA+GGG + chr4_3:4135723-4135742 Msa0625140:intron 30.0%
CTGTAGTATCTTGTCTATAT+CGG + chr4_3:4135945-4135964 Msa0625140:intron 30.0%
AATAGAAAACACTGTCACAA+AGG - chr4_3:4136052-4136071 None:intergenic 30.0%
GAAGATAAAAATGCTAGCTA+GGG - chr4_3:4136226-4136245 None:intergenic 30.0%
AGAAGATAAAAATGCTAGCT+AGG - chr4_3:4136227-4136246 None:intergenic 30.0%
! GAGAGGTTATTCTAAAGAAA+TGG + chr4_3:4136464-4136483 Msa0625140:intron 30.0%
AAAGGATTATCTCAGAACAT+GGG - chr4_3:4136619-4136638 None:intergenic 30.0%
AAAAGGATTATCTCAGAACA+TGG - chr4_3:4136620-4136639 None:intergenic 30.0%
! GATAATCCTTTTCTGTTTAG+TGG + chr4_3:4136628-4136647 Msa0625140:intron 30.0%
!!! CTTTTAATCGGTTTGAAGTT+TGG - chr4_3:4136960-4136979 None:intergenic 30.0%
! AAATGACACTTACGTGATTT+TGG + chr4_3:4137061-4137080 Msa0625140:intron 30.0%
! ATCTTAATCAGTTTGAGGTT+TGG - chr4_3:4137123-4137142 None:intergenic 30.0%
! CCAATTTGAAGTAAAACTCA+AGG - chr4_3:4137159-4137178 None:intergenic 30.0%
! CCTTGAGTTTTACTTCAAAT+TGG + chr4_3:4137156-4137175 Msa0625140:intron 30.0%
!!! TACAAGTGTACCTCTTTTTT+TGG - chr4_3:4137343-4137362 None:intergenic 30.0%
! TAAAAGAGGTACACTTGTTA+AGG + chr4_3:4137361-4137380 Msa0625140:intron 30.0%
TTAAGGATCTAAGGAGAAAA+GGG + chr4_3:4137378-4137397 Msa0625140:intron 30.0%
!!! TCTTTTTTGGTTATGATGTG+TGG + chr4_3:4137699-4137718 Msa0625140:three_prime_UTR 30.0%
GTAACGAAAACAAGTGTAAG+AGG + chr4_3:4135194-4135213 Msa0625140:five_prime_UTR 35.0%
CGTATCAGATAATCGATCAA+TGG + chr4_3:4135240-4135259 Msa0625140:exon 35.0%
! AATAAGATTGGCTGTCAAGT+TGG - chr4_3:4135326-4135345 None:intergenic 35.0%
ACTTGACAGCCAATCTTATT+AGG + chr4_3:4135326-4135345 Msa0625140:CDS 35.0%
!! GAGAGAATTTGAGTTTTGTG+AGG - chr4_3:4135367-4135386 None:intergenic 35.0%
ACTCAAATTCTCTCTCTTTC+TGG + chr4_3:4135373-4135392 Msa0625140:CDS 35.0%
ATAGAAGAAGAGAGAAAGAG+GGG - chr4_3:4135462-4135481 None:intergenic 35.0%
TTACCCTTCCTTCATTACTT+CGG + chr4_3:4135491-4135510 Msa0625140:intron 35.0%
GCTTAAACAATGTCTCTTGT+TGG + chr4_3:4135641-4135660 Msa0625140:intron 35.0%
TCTTGTTGGAACTTGAGTTT+GGG + chr4_3:4135655-4135674 Msa0625140:intron 35.0%
!! TTTTAGATTGAAGGTGTGAG+TGG + chr4_3:4135824-4135843 Msa0625140:intron 35.0%
!! AACCACATATTTTGTTGTGC+CGG - chr4_3:4135876-4135895 None:intergenic 35.0%
CATCTCAAAATTTGTCCCAT+AGG - chr4_3:4135990-4136009 None:intergenic 35.0%
! ACTACTTTGGAGAGAAATTG+AGG + chr4_3:4136018-4136037 Msa0625140:intron 35.0%
! CTACTTTGGAGAGAAATTGA+GGG + chr4_3:4136019-4136038 Msa0625140:intron 35.0%
AATACTCCACTTCACTTCAT+CGG - chr4_3:4136127-4136146 None:intergenic 35.0%
! TTCTAAAGAAATGGTGAGTG+TGG + chr4_3:4136473-4136492 Msa0625140:intron 35.0%
TCATTGCCACTAAACAGAAA+AGG - chr4_3:4136637-4136656 None:intergenic 35.0%
AGAAATGAAATGGAAACGCA+GGG - chr4_3:4136675-4136694 None:intergenic 35.0%
TCGACAAATAGATGTTACAC+AGG - chr4_3:4136832-4136851 None:intergenic 35.0%
ACATTCAACTCACACTTACA+TGG + chr4_3:4136891-4136910 Msa0625140:intron 35.0%
! AACTCACACTTACATGGTTT+TGG + chr4_3:4136897-4136916 Msa0625140:intron 35.0%
ACTGTGTGAAACTGTGTTTA+AGG - chr4_3:4137293-4137312 None:intergenic 35.0%
CACTTAGAAGCCAAAAAAAG+AGG + chr4_3:4137330-4137349 Msa0625140:intron 35.0%
! AAGAGGTACACTTGTAAAAG+AGG + chr4_3:4137347-4137366 Msa0625140:intron 35.0%
! GTACACTTGTTAAGGATCTA+AGG + chr4_3:4137369-4137388 Msa0625140:intron 35.0%
GTTAAGGATCTAAGGAGAAA+AGG + chr4_3:4137377-4137396 Msa0625140:intron 35.0%
CCATGCAAATCCAAAATTCA+GGG - chr4_3:4137731-4137750 None:intergenic 35.0%
ACCATGCAAATCCAAAATTC+AGG - chr4_3:4137732-4137751 None:intergenic 35.0%
!! GAGTTTTGTGAGGTTCCTTA+AGG - chr4_3:4135357-4135376 None:intergenic 40.0%
GAAATACTGTCGATCCATGT+GGG - chr4_3:4135421-4135440 None:intergenic 40.0%
ACATGGATCGACAGTATTTC+CGG + chr4_3:4135421-4135440 Msa0625140:CDS 40.0%
TAGAAGAAGAGAGAAAGAGG+GGG - chr4_3:4135461-4135480 None:intergenic 40.0%
CAACCGAAGTAATGAAGGAA+GGG - chr4_3:4135497-4135516 None:intergenic 40.0%
TCAACCGAAGTAATGAAGGA+AGG - chr4_3:4135498-4135517 None:intergenic 40.0%
TAGGTCAACCGAAGTAATGA+AGG - chr4_3:4135502-4135521 None:intergenic 40.0%
TTCATTACTTCGGTTGACCT+AGG + chr4_3:4135501-4135520 Msa0625140:intron 40.0%
GGCTTCAGACTATTGAGTAT+TGG + chr4_3:4135528-4135547 Msa0625140:intron 40.0%
! TTTCTAAGAACTCGGTATCC+GGG - chr4_3:4135579-4135598 None:intergenic 40.0%
! CTTTCTAAGAACTCGGTATC+CGG - chr4_3:4135580-4135599 None:intergenic 40.0%
CTCTTGTTGGAACTTGAGTT+TGG + chr4_3:4135654-4135673 Msa0625140:intron 40.0%
GCTTTGTAGAAACAAATGGG+TGG - chr4_3:4135719-4135738 None:intergenic 40.0%
! TATCGGTGCTTCATAGATAG+AGG + chr4_3:4135962-4135981 Msa0625140:intron 40.0%
! ATCGGTGCTTCATAGATAGA+GGG + chr4_3:4135963-4135982 Msa0625140:intron 40.0%
TTCATAGATAGAGGGACCTA+TGG + chr4_3:4135971-4135990 Msa0625140:intron 40.0%
TCATAGATAGAGGGACCTAT+GGG + chr4_3:4135972-4135991 Msa0625140:intron 40.0%
AAGAAACCGATGAAGTGAAG+TGG + chr4_3:4136118-4136137 Msa0625140:intron 40.0%
!! AATTTCTGGTGCCACAAGTT+AGG - chr4_3:4136534-4136553 None:intergenic 40.0%
!! GCGATGAGTACTGAAATTTC+TGG - chr4_3:4136548-4136567 None:intergenic 40.0%
TTCAGTACTCATCGCTACAA+TGG + chr4_3:4136553-4136572 Msa0625140:intron 40.0%
TACTCATCGCTACAATGGTT+AGG + chr4_3:4136558-4136577 Msa0625140:intron 40.0%
CAGAAATGAAATGGAAACGC+AGG - chr4_3:4136676-4136695 None:intergenic 40.0%
AGGGTAACTTCACATTACAG+TGG - chr4_3:4137026-4137045 None:intergenic 40.0%
GGCGTTAAATGCAAATCATC+AGG - chr4_3:4137480-4137499 None:intergenic 40.0%
! CCCTGAATTTTGGATTTGCA+TGG + chr4_3:4137728-4137747 Msa0625140:three_prime_UTR 40.0%
GATAATCGATCAATGGCGTC+AGG + chr4_3:4135247-4135266 Msa0625140:exon 45.0%
GCAGAGGAAAAGTAGCGATT+TGG + chr4_3:4135269-4135288 Msa0625140:CDS 45.0%
TTCAGTACTTGCTTCCCACA+TGG + chr4_3:4135404-4135423 Msa0625140:CDS 45.0%
GGAAATACTGTCGATCCATG+TGG - chr4_3:4135422-4135441 None:intergenic 45.0%
! ATAGTCTGAAGCCAAGACCT+AGG - chr4_3:4135521-4135540 None:intergenic 45.0%
!! CTATTGAGTATTGGAGCGCT+TGG + chr4_3:4135537-4135556 Msa0625140:intron 45.0%
! ATTGGAGCGCTTGGATTAAC+CGG + chr4_3:4135546-4135565 Msa0625140:intron 45.0%
! TTCTAAGAACTCGGTATCCG+GGG - chr4_3:4135578-4135597 None:intergenic 45.0%
GAGTGGTGTCTAACATGTGT+TGG + chr4_3:4135841-4135860 Msa0625140:intron 45.0%
CACCGGCACAACAAAATATG+TGG + chr4_3:4135871-4135890 Msa0625140:intron 45.0%
AGAAATGGTGAGTGTGGAGT+AGG + chr4_3:4136479-4136498 Msa0625140:intron 45.0%
TGTGGAGTAGGAACTAGGAA+TGG + chr4_3:4136491-4136510 Msa0625140:intron 45.0%
TGAAATGGAAACGCAGGGAA+GGG - chr4_3:4136670-4136689 None:intergenic 45.0%
ATGAAATGGAAACGCAGGGA+AGG - chr4_3:4136671-4136690 None:intergenic 45.0%
AGGATCTAAGGAGAAAAGGG+TGG + chr4_3:4137381-4137400 Msa0625140:intron 45.0%
!! CTCTGGGATGTCTGTATGAA+TGG - chr4_3:4137570-4137589 None:intergenic 45.0%
CATTCATACAGACATCCCAG+AGG + chr4_3:4137568-4137587 Msa0625140:CDS 45.0%
TCGCTGAAACGCATTCATGA+TGG + chr4_3:4137604-4137623 Msa0625140:exon 45.0%
! GAAGCTAATCTGAGATCTGC+TGG + chr4_3:4137626-4137645 Msa0625140:three_prime_UTR 45.0%
! TAGCGATTTGGAGAGCAGCA+GGG + chr4_3:4135281-4135300 Msa0625140:CDS 50.0%
! GGGGGTCTTACTGAGTTTTC+CGG - chr4_3:4135443-4135462 None:intergenic 50.0%
ACTTCGGTTGACCTAGGTCT+TGG + chr4_3:4135507-4135526 Msa0625140:intron 50.0%
GTGAGTGTGGAGTAGGAACT+AGG + chr4_3:4136486-4136505 Msa0625140:intron 50.0%
GACGTCGTCTTGATTCCTCT+GGG - chr4_3:4137586-4137605 None:intergenic 50.0%
! GTGGCAGATGCCCTGAATTT+TGG + chr4_3:4137718-4137737 Msa0625140:three_prime_UTR 50.0%
! GTAGCGATTTGGAGAGCAGC+AGG + chr4_3:4135280-4135299 Msa0625140:CDS 55.0%
GGATTAACCGGTCCTTTCCC+CGG + chr4_3:4135558-4135577 Msa0625140:intron 55.0%
AGAACTCGGTATCCGGGGAA+AGG - chr4_3:4135573-4135592 None:intergenic 55.0%
! CATGTGTTGGTGTGCGACAC+CGG + chr4_3:4135854-4135873 Msa0625140:intron 55.0%
CGACGTCGTCTTGATTCCTC+TGG - chr4_3:4137587-4137606 None:intergenic 55.0%
CGATCAATGGCGTCAGGCAG+AGG + chr4_3:4135253-4135272 Msa0625140:exon 60.0%
TCGGTATCCGGGGAAAGGAC+CGG - chr4_3:4135568-4135587 None:intergenic 60.0%
! AACGCAGGGAAGGGTGTGAG+TGG - chr4_3:4136661-4136680 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 4135183 4137772 4135183 ID=Msa0625140;Name=Msa0625140
chr4_3 mRNA 4135183 4137772 4135183 ID=Msa0625140-mRNA-1;Parent=Msa0625140;Name=Msa0625140-mRNA-1;_AED=0.00;_eAED=0.00;_QI=76|1|1|1|0|0.5|2|162|78
chr4_3 exon 4135183 4135451 4135183 ID=Msa0625140-mRNA-1:exon:984;Parent=Msa0625140-mRNA-1
chr4_3 exon 4137567 4137772 4137567 ID=Msa0625140-mRNA-1:exon:985;Parent=Msa0625140-mRNA-1
chr4_3 five_prime_UTR 4135183 4135258 4135183 ID=Msa0625140-mRNA-1:five_prime_utr;Parent=Msa0625140-mRNA-1
chr4_3 CDS 4135259 4135451 4135259 ID=Msa0625140-mRNA-1:cds;Parent=Msa0625140-mRNA-1
chr4_3 CDS 4137567 4137610 4137567 ID=Msa0625140-mRNA-1:cds;Parent=Msa0625140-mRNA-1
chr4_3 three_prime_UTR 4137611 4137772 4137611 ID=Msa0625140-mRNA-1:three_prime_utr;Parent=Msa0625140-mRNA-1
Gene Sequence

>Msa0625140

ATGGCGTCAGGCAGAGGAAAAGTAGCGATTTGGAGAGCAGCAGGGATGACATACACAGCATATTCCAACTTGACAGCCAATCTTATTAGGAATTGCCTTAAGGAACCTCACAAAACTCAAATTCTCTCTCTTTCTGGCGAGAACGTTCAGTACTTGCTTCCCACATGGATCGACAGTATTTCCGGAAAACTCACCATTCATACAGACATCCCAGAGGAATCAAGACGACGTCGCTGA

Protein sequence

>Msa0625140

MASGRGKVAIWRAAGMTYTAYSNLTANLIRNCLKEPHKTQILSLSGENVQYLLPTWIDSISGKLTIHTDIPEESRRRR*