AlfalfaGEDB | Msa0074320

M. sativa cultivar zhongmu-4 / Msa0074320

KEGG: K02918
GO: -
PFAM: PF00831.26

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0074320	sp\|Q9M5L0\|RL35_EUPES	90.984	122	11	0	20	141	1	122	2.61e-72	215

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0074320	A0A1S2XV31	97.561	123	3	0	20	142	1	123	2.72e-76	231

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0002490	Msa0074320	0.839754	1.311157e-57	-8.615850e-47
Msa0003720	Msa0074320	0.827010	1.980465e-54	-8.615850e-47
Msa0003780	Msa0074320	0.849898	2.405252e-60	-8.615850e-47
Msa0004950	Msa0074320	0.801941	7.100982e-49	-8.615850e-47
Msa0004980	Msa0074320	0.821675	3.559085e-53	-8.615850e-47
Msa0012080	Msa0074320	0.808025	3.791939e-50	-8.615850e-47
Msa0016670	Msa0074320	0.859563	3.793109e-63	-8.615850e-47
Msa0018620	Msa0074320	0.812550	4.006376e-51	-8.615850e-47
Msa0021810	Msa0074320	0.817432	3.307355e-52	-8.615850e-47
Msa0022970	Msa0074320	0.824389	8.289033e-54	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
Msa0074320	MtrunA17_Chr1g0182051	96.667	120	4	23	142	3	122	4.71e-77	224
Msa0074320	MtrunA17_Chr4g0030641	89.431	123	13	20	142	1	123	2.85e-73	214
Msa0074320	MtrunA17_Chr1g0212921	91.667	120	10	23	142	2	121	1.55e-72	213
Msa0074320	MtrunA17_Chr4g0036351	87.395	119	15	24	142	3	121	2.22e-67	199
Msa0074320	MtrunA17_Chr7g0249361	85.366	123	18	20	142	46	168	2.31e-67	201
Msa0074320	MtrunA17_Chr1g0206641	88.889	36	4	33	68	5	40	8.20e-14	61.6
Msa0074320	MtrunA17_Chr5g0419591	87.879	33	4	86	118	2	34	5.22e-13	59.7

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0074320	AT5G02610.1	88.525	122	14	0	20	141	1	122	1.39e-70	208
Msa0074320	AT3G09500.1	88.525	122	14	0	20	141	1	122	2.90e-70	207
Msa0074320	AT2G39390.1	88.525	122	14	0	20	141	1	122	4.21e-70	207
Msa0074320	AT3G55170.5	86.066	122	17	0	20	141	1	122	2.72e-68	202
Msa0074320	AT3G55170.2	86.066	122	17	0	20	141	1	122	2.72e-68	202
Msa0074320	AT3G55170.1	86.066	122	17	0	20	141	1	122	2.72e-68	202
Msa0074320	AT5G02610.2	74.483	145	14	1	20	141	1	145	9.06e-65	194
Msa0074320	AT3G55170.4	85.859	99	14	0	20	118	1	99	2.52e-52	161
Msa0074320	AT3G55170.3	85.859	99	14	0	20	118	1	99	2.52e-52	161

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Find 32 sgRNAs with CRISPR-Local

Find 312 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGTATGCAATCAAAGCTT+AGG	0.473169	1_2:-48746474	Msa0074320:exon
AAGACGCCTTACCAAACACC+AGG	0.624051	1_2:-48747894	Msa0074320:intron
AAGGCGTCTTCTGATAGCAC+GGG	0.605672	1_2:+48747907	None:intergenic
AATTCATTAACTTGTATGGG+AGG	0.562957	1_2:+48746179	None:intergenic
ACGGCTATGGAGTGATAGTG+AGG	0.672283	1_2:+48748398	None:intergenic
AGGTGCACCGCCGGTGACTT+TGG	0.448506	1_2:+48748161	None:intergenic
AGTGTTTAAGTAGTCTTGAA+AGG	0.499675	1_2:+48746208	None:intergenic
AGTTCAATATTTCAAATAAC+TGG	0.271293	1_2:+48746426	None:intergenic
ATCTATCTTGAGACTTCCGA+AGG	0.580195	1_2:-48746303	Msa0074320:three_prime_UTR
ATGCAATCAAAGCTTAGGCG+AGG	0.662311	1_2:-48746469	Msa0074320:exon

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATAACACATCGTCGTTAAAA+TGG	-	chr1_2:48746241-48746260	Msa0074320:three_prime_UTR	30.0%
CGTTCCTGAATCAAAAAAAA+TGG	-	chr1_2:48746318-48746337	Msa0074320:three_prime_UTR	30.0%
AATTCTCACTTCAAATGCAA+TGG	+	chr1_2:48746427-48746446	None:intergenic	30.0%
TTATATGGAAGCTGCTTATA+TGG	-	chr1_2:48746598-48746617	Msa0074320:intron	30.0%
AATCATTGCTACAACACTAT+TGG	+	chr1_2:48746896-48746915	None:intergenic	30.0%
TCATAGAGATATAACAAGTG+TGG	-	chr1_2:48747105-48747124	Msa0074320:intron	30.0%
GTTAGCTAAATTATCGATGT+GGG	+	chr1_2:48747164-48747183	None:intergenic	30.0%
TGTTAGCTAAATTATCGATG+TGG	+	chr1_2:48747165-48747184	None:intergenic	30.0%
TGCAAATTCTTGAAGAATTC+TGG	-	chr1_2:48747372-48747391	Msa0074320:intron	30.0%
TATTCTACATTGTAGCAGTT+AGG	-	chr1_2:48747527-48747546	Msa0074320:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_2	gene	48746163	48748555	48746163	ID=Msa0074320;Name=Msa0074320
chr1_2	mRNA	48746163	48748555	48746163	ID=Msa0074320-mRNA-1;Parent=Msa0074320;Name=Msa0074320-mRNA-1;_AED=0.03;_eAED=0.03;_QI=0\|0.75\|0.6\|0.8\|1\|1\|5\|312\|142
chr1_2	exon	48746163	48746549	48746163	ID=Msa0074320-mRNA-1:exon:14904;Parent=Msa0074320-mRNA-1
chr1_2	exon	48747895	48748051	48747895	ID=Msa0074320-mRNA-1:exon:14903;Parent=Msa0074320-mRNA-1
chr1_2	exon	48748144	48748279	48748144	ID=Msa0074320-mRNA-1:exon:14902;Parent=Msa0074320-mRNA-1
chr1_2	exon	48748378	48748433	48748378	ID=Msa0074320-mRNA-1:exon:14901;Parent=Msa0074320-mRNA-1
chr1_2	exon	48748551	48748555	48748551	ID=Msa0074320-mRNA-1:exon:14900;Parent=Msa0074320-mRNA-1
chr1_2	CDS	48748551	48748555	48748551	ID=Msa0074320-mRNA-1:cds;Parent=Msa0074320-mRNA-1
chr1_2	CDS	48748378	48748433	48748378	ID=Msa0074320-mRNA-1:cds;Parent=Msa0074320-mRNA-1
chr1_2	CDS	48748144	48748279	48748144	ID=Msa0074320-mRNA-1:cds;Parent=Msa0074320-mRNA-1
chr1_2	CDS	48747895	48748051	48747895	ID=Msa0074320-mRNA-1:cds;Parent=Msa0074320-mRNA-1
chr1_2	CDS	48746475	48746549	48746475	ID=Msa0074320-mRNA-1:cds;Parent=Msa0074320-mRNA-1
chr1_2	three_prime_UTR	48746163	48746474	48746163	ID=Msa0074320-mRNA-1:three_prime_utr;Parent=Msa0074320-mRNA-1

Gene Sequence

>Msa0074320

ATGGGGGTTTCTTCAGTTCCTCACTATCACTCCATAGCCGTTCCTGAATCAAAAAAAATGGTGAGAATTAAGGTGTTCGAGTTGAGGAACAAACCCAAAGCTGATTTGCTGAACCAGCTGAAGGATCTGAAGGCGGAGCTCGCTTTGCTTCGAGTCGCCAAAGTCACCGGCGGTGCACCTAACAAGCTCTCCAAAATCAAAGTTGTGAGGCTTTCAATTGCACAAGTGTTGACAGTGATTTCACAGAAGCAGAAAGCTGCGCTCAGAGAGGTTTACAAAAATAAGAAGTATTTACCACTTGATCTTCGTCCTAAGAAGACCCGTGCTATCAGAAGACGCCTTACCAAACACCAGGCATCGTTGAAGACAGAGCGAGAGAAGAAGAAGGAGATATACTTTCCACTAAGAAAGTATGCAATCAAAGCTTAG

Protein sequence

>Msa0074320

MGVSSVPHYHSIAVPESKKMVRIKVFELRNKPKADLLNQLKDLKAELALLRVAKVTGGAPNKLSKIKVVRLSIAQVLTVISQKQKAALREVYKNKKYLPLDLRPKKTRAIRRRLTKHQASLKTEREKKKEIYFPLRKYAIKA*