Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0187690 | XP_004494985.1 | 97.561 | 123 | 3 | 0 | 1 | 123 | 1 | 123 | 5.14e-76 | 231 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0187690 | sp|Q9M5L0|RL35_EUPES | 90.984 | 122 | 11 | 0 | 1 | 122 | 1 | 122 | 1.94e-72 | 214 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0187690 | A0A1S2XV31 | 97.561 | 123 | 3 | 0 | 1 | 123 | 1 | 123 | 2.45e-76 | 231 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0002490 | Msa0187690 | 0.836256 | 1.040701e-56 | -8.615850e-47 |
| Msa0002750 | Msa0187690 | 0.802923 | 4.454475e-49 | -8.615850e-47 |
| Msa0003720 | Msa0187690 | 0.819370 | 1.203631e-52 | -8.615850e-47 |
| Msa0003780 | Msa0187690 | 0.857992 | 1.118030e-62 | -8.615850e-47 |
| Msa0004950 | Msa0187690 | 0.804345 | 2.258547e-49 | -8.615850e-47 |
| Msa0004980 | Msa0187690 | 0.827363 | 1.630829e-54 | -8.615850e-47 |
| Msa0016670 | Msa0187690 | 0.861185 | 1.224836e-63 | -8.615850e-47 |
| Msa0018620 | Msa0187690 | 0.804903 | 1.727552e-49 | -8.615850e-47 |
| Msa0021810 | Msa0187690 | 0.815054 | 1.125371e-51 | -8.615850e-47 |
| Msa0022970 | Msa0187690 | 0.823626 | 1.251966e-53 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0187690 | MtrunA17_Chr1g0182051 | 96.667 | 120 | 4 | 0 | 4 | 123 | 3 | 122 | 2.78e-77 | 224 |
| Msa0187690 | MtrunA17_Chr4g0030641 | 89.431 | 123 | 13 | 0 | 1 | 123 | 1 | 123 | 2.06e-73 | 214 |
| Msa0187690 | MtrunA17_Chr1g0212921 | 91.667 | 120 | 10 | 0 | 4 | 123 | 2 | 121 | 5.06e-73 | 213 |
| Msa0187690 | MtrunA17_Chr4g0036351 | 87.395 | 119 | 15 | 0 | 5 | 123 | 3 | 121 | 1.16e-67 | 199 |
| Msa0187690 | MtrunA17_Chr7g0249361 | 85.366 | 123 | 18 | 0 | 1 | 123 | 46 | 168 | 1.52e-67 | 201 |
| Msa0187690 | MtrunA17_Chr1g0206641 | 88.889 | 36 | 4 | 0 | 14 | 49 | 5 | 40 | 4.97e-14 | 61.6 |
| Msa0187690 | MtrunA17_Chr5g0419591 | 87.879 | 33 | 4 | 0 | 67 | 99 | 2 | 34 | 2.72e-13 | 59.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0187690 | AT5G02610.1 | 88.525 | 122 | 14 | 0 | 1 | 122 | 1 | 122 | 5.75e-71 | 208 |
| Msa0187690 | AT2G39390.1 | 88.525 | 122 | 14 | 0 | 1 | 122 | 1 | 122 | 1.20e-70 | 207 |
| Msa0187690 | AT3G09500.1 | 88.525 | 122 | 14 | 0 | 1 | 122 | 1 | 122 | 1.32e-70 | 207 |
| Msa0187690 | AT3G55170.5 | 86.066 | 122 | 17 | 0 | 1 | 122 | 1 | 122 | 1.11e-68 | 202 |
| Msa0187690 | AT3G55170.2 | 86.066 | 122 | 17 | 0 | 1 | 122 | 1 | 122 | 1.11e-68 | 202 |
| Msa0187690 | AT3G55170.1 | 86.066 | 122 | 17 | 0 | 1 | 122 | 1 | 122 | 1.11e-68 | 202 |
| Msa0187690 | AT5G02610.2 | 74.483 | 145 | 14 | 1 | 1 | 122 | 1 | 145 | 3.72e-65 | 194 |
| Msa0187690 | AT3G55170.4 | 85.859 | 99 | 14 | 0 | 1 | 99 | 1 | 99 | 6.81e-53 | 162 |
| Msa0187690 | AT3G55170.3 | 85.859 | 99 | 14 | 0 | 1 | 99 | 1 | 99 | 6.81e-53 | 162 |
Find 34 sgRNAs with CRISPR-Local
Find 284 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGTATGCAATCAAAGCTT+AGG | 0.473169 | 2_1:+2401119 | Msa0187690:CDS |
| AAGACGCCTTACCAAACACC+AGG | 0.624051 | 2_1:+2399700 | Msa0187690:CDS |
| AAGGCGTCTTCTGATAGCAC+GGG | 0.605672 | 2_1:-2399687 | None:intergenic |
| AATTCATTAACTTGTATGGG+AGG | 0.562957 | 2_1:-2401414 | None:intergenic |
| ACCCTACAGTTAAAATCGCA+AGG | 0.605131 | 2_1:-2399165 | None:intergenic |
| ACGGCTATGGAGTGATAGTG+AGG | 0.672283 | 2_1:-2399198 | None:intergenic |
| AGGTGCACCGCCGGTGACTT+TGG | 0.448506 | 2_1:-2399435 | None:intergenic |
| AGTGTTTAAGTAGTCTTGAA+AGG | 0.499675 | 2_1:-2401385 | None:intergenic |
| AGTTCAATATTTCAAATAAC+TGG | 0.271293 | 2_1:-2401167 | None:intergenic |
| ATAACACATCGTCGTTAAAA+TGG | 0.393667 | 2_1:+2399141 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| CGTTCCTGAATCAAAAAAAA+TGG | + | chr2_1:2399218-2399237 | Msa0187690:exon | 30.0% | |
| AATTCTCACTTCAAATGCAA+TGG | - | chr2_1:2399327-2399346 | None:intergenic | 30.0% | |
| TTATATGGAAGCTGCTTATA+TGG | + | chr2_1:2399496-2399515 | Msa0187690:intron | 30.0% | |
| AATCATTGCTACAACACTAT+TGG | - | chr2_1:2399794-2399813 | None:intergenic | 30.0% | |
| TCATAGAGATATAACAAGTG+TGG | + | chr2_1:2400003-2400022 | Msa0187690:intron | 30.0% | |
| GTTAGCTAAATTATCGATGT+GGG | - | chr2_1:2400062-2400081 | None:intergenic | 30.0% | |
| TGTTAGCTAAATTATCGATG+TGG | - | chr2_1:2400063-2400082 | None:intergenic | 30.0% | |
| TGCAAATTCTTGAAGAATTC+TGG | + | chr2_1:2400270-2400289 | Msa0187690:intron | 30.0% | |
| TATTCTACATTGTAGCAGTT+AGG | + | chr2_1:2400424-2400443 | Msa0187690:intron | 30.0% | |
| ATTCTACATTGTAGCAGTTA+GGG | + | chr2_1:2400425-2400444 | Msa0187690:intron | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_1 | gene | 2399155 | 2401452 | 2399155 | ID=Msa0187690;Name=Msa0187690 |
| chr2_1 | mRNA | 2399155 | 2401452 | 2399155 | ID=Msa0187690-mRNA-1;Parent=Msa0187690;Name=Msa0187690-mRNA-1;_AED=0.06;_eAED=0.06;_QI=82|1|1|1|1|1|4|312|123 |
| chr2_1 | exon | 2399155 | 2399240 | 2399155 | ID=Msa0187690-mRNA-1:exon:1155;Parent=Msa0187690-mRNA-1 |
| chr2_1 | exon | 2399339 | 2399474 | 2399339 | ID=Msa0187690-mRNA-1:exon:1156;Parent=Msa0187690-mRNA-1 |
| chr2_1 | exon | 2399565 | 2399721 | 2399565 | ID=Msa0187690-mRNA-1:exon:1157;Parent=Msa0187690-mRNA-1 |
| chr2_1 | exon | 2401066 | 2401452 | 2401066 | ID=Msa0187690-mRNA-1:exon:1158;Parent=Msa0187690-mRNA-1 |
| chr2_1 | five_prime_UTR | 2399155 | 2399236 | 2399155 | ID=Msa0187690-mRNA-1:five_prime_utr;Parent=Msa0187690-mRNA-1 |
| chr2_1 | CDS | 2399237 | 2399240 | 2399237 | ID=Msa0187690-mRNA-1:cds;Parent=Msa0187690-mRNA-1 |
| chr2_1 | CDS | 2399339 | 2399474 | 2399339 | ID=Msa0187690-mRNA-1:cds;Parent=Msa0187690-mRNA-1 |
| chr2_1 | CDS | 2399565 | 2399721 | 2399565 | ID=Msa0187690-mRNA-1:cds;Parent=Msa0187690-mRNA-1 |
| chr2_1 | CDS | 2401066 | 2401140 | 2401066 | ID=Msa0187690-mRNA-1:cds;Parent=Msa0187690-mRNA-1 |
| chr2_1 | three_prime_UTR | 2401141 | 2401452 | 2401141 | ID=Msa0187690-mRNA-1:three_prime_utr;Parent=Msa0187690-mRNA-1 |
| Gene Sequence |
>Msa0187690 ATGGTGAGAATTAAGGTGTTCGAGTTGAGGAACAAACCCAAAGCTGATTTGCTGAACCAGCTGAAGGATCTGAAGGCGGAGCTCGCTTTGCTTCGAGTCGCCAAAGTCACCGGCGGTGCACCTAACAAGCTCTCCAAAATCAAAGTTGTGAGGCTTTCAATTGCACAAGTGTTGACAGTGATTTCACAGAAGCAGAAAGCTGCGCTCAGAGAGGTTTACAAAAATAAGAAGTATTTACCACTTGATCTTCGTCCTAAGAAGACCCGTGCTATCAGAAGACGCCTTACCAAACACCAGGCATCGTTGAAGACAGAGCGAGAGAAGAAGAAGGAGATATACTTTCCACTAAGAAAGTATGCAATCAAAGCTTAG |
| Protein sequence |
>Msa0187690 MVRIKVFELRNKPKADLLNQLKDLKAELALLRVAKVTGGAPNKLSKIKVVRLSIAQVLTVISQKQKAALREVYKNKKYLPLDLRPKKTRAIRRRLTKHQASLKTEREKKKEIYFPLRKYAIKA* |
