AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0661620

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0661620 MtrunA17_Chr3g0144051 91.667 132 10 1 1 131 1 132 7.61e-74 219
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0661620 AT1G20770.1 57.143 84 31 2 22 101 30 112 1.24e-27 102
Msa0661620 AT1G20770.2 57.143 84 31 2 22 101 30 112 4.30e-27 99.8

Find 59 sgRNAs with CRISPR-Local

Find 200 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAAGTCGCTTTGCAATTTA+TGG 0.047989 4_3:+73107445 Msa0661620:CDS
GCGCGTTAATAGTTCTTTAT+TGG 0.185278 4_3:+73107833 Msa0661620:CDS
TGTAATTTCAACTTTATATC+AGG 0.192109 4_3:+73108320 Msa0661620:three_prime_UTR
TCTTCTAATTTAGTACTTTC+AGG 0.205802 4_3:-73108132 None:intergenic
ATTGCTACTTCTTCATTCTT+TGG 0.255169 4_3:-73109235 None:intergenic
AGCTAGTGCTAGATATTCTA+TGG 0.274041 4_3:+73107803 Msa0661620:CDS
GTCAATGGGGAAAACATATT+TGG 0.285598 4_3:-73108221 None:intergenic
CACACTTCACGTTGCAGAAA+TGG 0.314124 4_3:+73108091 Msa0661620:CDS
GACCGAGCATTGCAAGATTT+TGG 0.314762 4_3:+73110477 Msa0661620:three_prime_UTR
GTTGGGTCGCACGAAGCTTT+GGG 0.323316 4_3:-73109000 None:intergenic
TCCTGTTAAGTTCTCTGTTA+TGG 0.337078 4_3:-73108466 None:intergenic
AGTTGGGTCGCACGAAGCTT+TGG 0.351397 4_3:-73109001 None:intergenic
GAGAAGAGAGGTTTGTTTGA+AGG 0.357896 4_3:+73107391 Msa0661620:CDS
TTGCATTATGTTCAGAAGAT+AGG 0.359977 4_3:-73108272 None:intergenic
CTCCATTTGTGATGCCGCTC+TGG 0.374189 4_3:-73110445 None:intergenic
AGAAGAGAGGTTTGTTTGAA+GGG 0.396297 4_3:+73107392 Msa0661620:CDS
ACCATAACAGAGAACTTAAC+AGG 0.406915 4_3:+73108465 Msa0661620:three_prime_UTR
CTTCCACCTCTTGATGAAGT+TGG 0.430151 4_3:-73109209 None:intergenic
TGAAGAAGTAGCAATCCTTC+AGG 0.436833 4_3:+73109243 Msa0661620:three_prime_UTR
TATCGATGCCAGCAGTCAAT+GGG 0.440676 4_3:-73108235 None:intergenic
ACACTTCACGTTGCAGAAAT+GGG 0.458799 4_3:+73108092 Msa0661620:CDS
AGACCAAATGAAAAGTTTCT+CGG 0.463551 4_3:+73110520 Msa0661620:three_prime_UTR
AGTAATAGTATGAAATCATC+TGG 0.464799 4_3:+73108177 Msa0661620:CDS
ATATTCATCACAGGGATGGC+TGG 0.466276 4_3:+73107776 Msa0661620:intron
CTATCGATGCCAGCAGTCAA+TGG 0.475477 4_3:-73108236 None:intergenic
TGCCAGAGCGGCATCACAAA+TGG 0.476797 4_3:+73110443 Msa0661620:three_prime_UTR
AATCTCAAGTTGAATCAAGT+TGG 0.477106 4_3:+73110619 Msa0661620:three_prime_UTR
GCTGGCATCGATAGTGTAAA+GGG 0.480873 4_3:+73108245 Msa0661620:three_prime_UTR
ACCTTTCTCAAATGACAGTT+GGG 0.486844 4_3:-73109017 None:intergenic
CACCTTTCTCAAATGACAGT+TGG 0.489015 4_3:-73109018 None:intergenic
ATCCCGAAAATCATAGTGAA+TGG 0.490710 4_3:+73107361 Msa0661620:exon
GTAATTTCAACTTTATATCA+GGG 0.501664 4_3:+73108321 Msa0661620:three_prime_UTR
CACCAAAATCTTGCAATGCT+CGG 0.503585 4_3:-73110479 None:intergenic
TGCTGGCATCGATAGTGTAA+AGG 0.510744 4_3:+73108244 Msa0661620:three_prime_UTR
TAGCAGCTGAATGGAATTTG+AGG 0.516503 4_3:-73107857 None:intergenic
TGGAGGAGAGAGAATCGGAA+AGG 0.522502 4_3:-73107490 None:intergenic
TCAATGTTGTAGCAGCTGAA+TGG 0.524811 4_3:-73107866 None:intergenic
TCTCTCCTCCACACTTCGAC+AGG 0.526939 4_3:+73107502 Msa0661620:CDS
GGAGGAGAGAGAATCGGAAA+GGG 0.529857 4_3:-73107489 None:intergenic
ATACTACTAAAAGCATATAG+TGG 0.535069 4_3:-73108394 None:intergenic
AAGTGTGGAGGAGAGAGAAT+CGG 0.545779 4_3:-73107495 None:intergenic
ATAGTGGCAAAATCATATTA+CGG 0.550281 4_3:-73108378 None:intergenic
TTGGGTCGCACGAAGCTTTG+GGG 0.558578 4_3:-73108999 None:intergenic
AATGGAAGAAGAGAGAAGAG+AGG 0.571337 4_3:+73107379 Msa0661620:exon
ACTTTCAGGTTCGTGTTCAG+AGG 0.572436 4_3:-73108118 None:intergenic
ACCCAACTGTCATTTGAGAA+AGG 0.572755 4_3:+73109016 Msa0661620:three_prime_UTR
AGACAAGAATGAGCAGCCAC+AGG 0.580041 4_3:+73108152 Msa0661620:CDS
TTCATACTATTACTATCCTG+TGG 0.594361 4_3:-73108168 None:intergenic
AAGATATCCGAAAATCAAGA+TGG 0.595526 4_3:+73107888 Msa0661620:CDS
CTACAAACAGTGTAAAAGTG+TGG 0.621576 4_3:+73107310 None:intergenic
GCTAGTGCTAGATATTCTAT+GGG 0.628225 4_3:+73107804 Msa0661620:CDS
TAACCAACTTCATCAAGAGG+TGG 0.630984 4_3:+73109206 Msa0661620:three_prime_UTR
CATTTCTGCAACGTGAAGTG+TGG 0.652174 4_3:-73108090 None:intergenic
GTAAAAGTGTGGAAATCTAG+CGG 0.657247 4_3:+73107321 Msa0661620:five_prime_UTR
TGTATGTACCTGTCGAAGTG+TGG 0.657763 4_3:-73107510 None:intergenic
CTGGCATCGATAGTGTAAAG+GGG 0.665023 4_3:+73108246 Msa0661620:three_prime_UTR
TGGCATCGATAGTGTAAAGG+GGG 0.684960 4_3:+73108247 Msa0661620:three_prime_UTR
ATCGATGCCAGCAGTCAATG+GGG 0.713158 4_3:-73108234 None:intergenic
ATGTACCTGTCGAAGTGTGG+AGG 0.713176 4_3:-73107507 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTAATAATAACCTTT+TGG + chr4_3:73108737-73108756 Msa0661620:intron 10.0%
!!! ACATTTACAATATTTTTTTA+CGG + chr4_3:73110022-73110041 Msa0661620:intron 10.0%
!! ATTTAAAAAGATAAGTTATT+GGG + chr4_3:73110341-73110360 Msa0661620:intron 10.0%
!!! AATTTTGTCTTTTATAGTTA+AGG + chr4_3:73107913-73107932 Msa0661620:intron 15.0%
!!! AGATTCTTTTAAAAAGATAA+TGG + chr4_3:73108548-73108567 Msa0661620:intron 15.0%
!!! TTTAACAAAAGATCTTTTAA+AGG + chr4_3:73108632-73108651 Msa0661620:intron 15.0%
!!! AACATAATTAGTTTTTACAT+AGG + chr4_3:73109531-73109550 Msa0661620:intron 15.0%
!!! CATTTACAATATTTTTTTAC+GGG + chr4_3:73110023-73110042 Msa0661620:intron 15.0%
!! GATTTAAAAAGATAAGTTAT+TGG + chr4_3:73110340-73110359 Msa0661620:intron 15.0%
!!! AATTTCTTATTCTTTTATTC+AGG + chr4_3:73110419-73110438 Msa0661620:intron 15.0%
!!! TTTTTTCAGTTTATTACTTT+TGG + chr4_3:73110654-73110673 Msa0661620:three_prime_UTR 15.0%
!!! AAATTTACCATCTTGATTTT+CGG - chr4_3:73107898-73107917 None:intergenic 20.0%
!! TGTAATTTCAACTTTATATC+AGG + chr4_3:73108320-73108339 Msa0661620:three_prime_UTR 20.0%
!! GTAATTTCAACTTTATATCA+GGG + chr4_3:73108321-73108340 Msa0661620:three_prime_UTR 20.0%
!! TTCAAAAGAGCTATAATAAA+CGG - chr4_3:73108576-73108595 None:intergenic 20.0%
!!! TTTAAGTATTTTCATCCTTT+TGG + chr4_3:73108703-73108722 Msa0661620:intron 20.0%
!! AAAAAAAAAGTTGTACCAAA+AGG - chr4_3:73108721-73108740 None:intergenic 20.0%
!! TTGAATTACTGTTAAAATCT+TGG + chr4_3:73108915-73108934 Msa0661620:intron 20.0%
!!! ATGTTTGATTTTAAGCAAAA+AGG - chr4_3:73109516-73109535 None:intergenic 20.0%
!! AATAACTTTATACAACACTT+TGG - chr4_3:73109638-73109657 None:intergenic 20.0%
!! ATAAAGTTATTCTTCAAAAC+AGG + chr4_3:73109647-73109666 Msa0661620:intron 20.0%
!! AAAAAAAAATACATAGGCTT+GGG - chr4_3:73109782-73109801 None:intergenic 20.0%
!! TAAAAAAAAATACATAGGCT+TGG - chr4_3:73109783-73109802 None:intergenic 20.0%
!!! GCCTATGTATTTTTTTTTAA+TGG + chr4_3:73109784-73109803 Msa0661620:intron 20.0%
!! GCCATTAAAAAAAAATACAT+AGG - chr4_3:73109788-73109807 None:intergenic 20.0%
!!! TTTTTTTTAATGGCAAATGT+TGG + chr4_3:73109794-73109813 Msa0661620:intron 20.0%
!! AATTACAGGAATTTAATTCA+AGG - chr4_3:73110230-73110249 None:intergenic 20.0%
!! TTGAATTAAATTCCTGTAAT+TGG + chr4_3:73110229-73110248 Msa0661620:intron 20.0%
!! TGAATTAAATTCCTGTAATT+GGG + chr4_3:73110230-73110249 Msa0661620:intron 20.0%
! GAATTTGATTAGGTTATGTA+TGG - chr4_3:73107541-73107560 None:intergenic 25.0%
! GAGTTGAATTGAATTTGATT+AGG - chr4_3:73107551-73107570 None:intergenic 25.0%
! AATAAGCTGAAAACAACTTA+TGG + chr4_3:73107677-73107696 Msa0661620:intron 25.0%
! TGTGATGAATATCTTAAAGA+AGG - chr4_3:73107768-73107787 None:intergenic 25.0%
!!! TTCTTTAAGATATTCATCAC+AGG + chr4_3:73107767-73107786 Msa0661620:intron 25.0%
!!! TCTTTAAGATATTCATCACA+GGG + chr4_3:73107768-73107787 Msa0661620:intron 25.0%
! GTACATGTTATGTTACAAAA+AGG - chr4_3:73108001-73108020 None:intergenic 25.0%
! TGGTTCATATATTAAAACAG+AGG - chr4_3:73108044-73108063 None:intergenic 25.0%
! TCTTCTAATTTAGTACTTTC+AGG - chr4_3:73108135-73108154 None:intergenic 25.0%
! AGTAATAGTATGAAATCATC+TGG + chr4_3:73108177-73108196 Msa0661620:CDS 25.0%
! ATAGTGGCAAAATCATATTA+CGG - chr4_3:73108381-73108400 None:intergenic 25.0%
!! ATACTACTAAAAGCATATAG+TGG - chr4_3:73108397-73108416 None:intergenic 25.0%
!!! TTGTTTCTCATTTTCTTACT+TGG + chr4_3:73108422-73108441 Msa0661620:three_prime_UTR 25.0%
! TCAAAAGAGCTATAATAAAC+GGG - chr4_3:73108575-73108594 None:intergenic 25.0%
! AAAAAAAAAGTTGTGCCAAA+AGG - chr4_3:73108755-73108774 None:intergenic 25.0%
! GGGTGAATAAATTATAGATT+TGG + chr4_3:73108855-73108874 Msa0661620:intron 25.0%
! AATTACTGTTAAAATCTTGG+TGG + chr4_3:73108918-73108937 Msa0661620:intron 25.0%
! ACACAAAGTAAGGAAATTTA+AGG - chr4_3:73109061-73109080 None:intergenic 25.0%
! AGATAAAATAAGGAGAAATG+AGG - chr4_3:73109278-73109297 None:intergenic 25.0%
! AGTAGAAGAGAGATAAAATA+AGG - chr4_3:73109288-73109307 None:intergenic 25.0%
! ATAAGTTCATATCAAGACTA+TGG + chr4_3:73109324-73109343 Msa0661620:intron 25.0%
!!! TTAGTTTTTACATAGGAACT+AGG + chr4_3:73109538-73109557 Msa0661620:intron 25.0%
! AAACATTCACTTAGCATTTA+GGG - chr4_3:73109915-73109934 None:intergenic 25.0%
! TAAACATTCACTTAGCATTT+AGG - chr4_3:73109916-73109935 None:intergenic 25.0%
! ATGTCACAAATTGATTGATA+AGG + chr4_3:73109954-73109973 Msa0661620:intron 25.0%
! TTTACGGGATAACATTTATA+AGG + chr4_3:73110038-73110057 Msa0661620:intron 25.0%
! TTACGGGATAACATTTATAA+GGG + chr4_3:73110039-73110058 Msa0661620:intron 25.0%
! AATTTAATTCAAGGTCTGTT+TGG - chr4_3:73110221-73110240 None:intergenic 25.0%
! GAATTAAATTCCTGTAATTG+GGG + chr4_3:73110231-73110250 Msa0661620:intron 25.0%
! TTCCATTCACTATGATTTTC+GGG - chr4_3:73107366-73107385 None:intergenic 30.0%
! CTTCCATTCACTATGATTTT+CGG - chr4_3:73107367-73107386 None:intergenic 30.0%
AACAATTTCAGATGATGCAA+TGG + chr4_3:73107721-73107740 Msa0661620:intron 30.0%
AGGTTATCAAATCAACAATC+AGG - chr4_3:73107748-73107767 None:intergenic 30.0%
AAGATATCCGAAAATCAAGA+TGG + chr4_3:73107888-73107907 Msa0661620:CDS 30.0%
TTCATACTATTACTATCCTG+TGG - chr4_3:73108171-73108190 None:intergenic 30.0%
TTGCATTATGTTCAGAAGAT+AGG - chr4_3:73108275-73108294 None:intergenic 30.0%
TTCTAATATCTTTGCTCTCA+TGG + chr4_3:73108825-73108844 Msa0661620:intron 30.0%
!! AAAATCTTGGTGGTTTTCAT+AGG + chr4_3:73108928-73108947 Msa0661620:intron 30.0%
AAAACTGCATACACAAAGTA+AGG - chr4_3:73109071-73109090 None:intergenic 30.0%
! TTTTAACCAACTTCATCAAG+AGG + chr4_3:73109203-73109222 Msa0661620:three_prime_UTR 30.0%
! ATTGCTACTTCTTCATTCTT+TGG - chr4_3:73109238-73109257 None:intergenic 30.0%
!! TCTCTCTTCTACTCTTATTT+TGG + chr4_3:73109295-73109314 Msa0661620:intron 30.0%
! TCAGTCCCTTTTCTTTAATA+TGG + chr4_3:73109487-73109506 Msa0661620:intron 30.0%
GGTCTCCATATTAAAGAAAA+GGG - chr4_3:73109495-73109514 None:intergenic 30.0%
AGGTCTCCATATTAAAGAAA+AGG - chr4_3:73109496-73109515 None:intergenic 30.0%
AAACACCTTATGTTATACGA+TGG - chr4_3:73109568-73109587 None:intergenic 30.0%
TTTATACAACACTTTGGTTG+AGG - chr4_3:73109632-73109651 None:intergenic 30.0%
TGTCAAATAATAAACCACAG+TGG + chr4_3:73109714-73109733 Msa0661620:intron 30.0%
AAATAATAAACCACAGTGGA+TGG + chr4_3:73109718-73109737 Msa0661620:intron 30.0%
AAACGATGAGTAATGTTATG+AGG + chr4_3:73109742-73109761 Msa0661620:intron 30.0%
AACGATGAGTAATGTTATGA+GGG + chr4_3:73109743-73109762 Msa0661620:intron 30.0%
!!! ATTTTTTTAACTCCTTCCTC+TGG + chr4_3:73109828-73109847 Msa0661620:intron 30.0%
!!! TTTTTTTAACTCCTTCCTCT+GGG + chr4_3:73109829-73109848 Msa0661620:intron 30.0%
AACATTCACTTAGCATTTAG+GGG - chr4_3:73109914-73109933 None:intergenic 30.0%
! GTTTACCCTTGTTTGATATT+TGG + chr4_3:73109931-73109950 Msa0661620:intron 30.0%
GACATCCAAATATCAAACAA+GGG - chr4_3:73109939-73109958 None:intergenic 30.0%
TGACATCCAAATATCAAACA+AGG - chr4_3:73109940-73109959 None:intergenic 30.0%
TTGTAAAATGAGAAGCGAAT+GGG - chr4_3:73110094-73110113 None:intergenic 30.0%
TAGAAAAGCCATATTGTGAT+CGG - chr4_3:73110122-73110141 None:intergenic 30.0%
AGACCAAATGAAAAGTTTCT+CGG + chr4_3:73110520-73110539 Msa0661620:three_prime_UTR 30.0%
!! AATCTCAAGTTGAATCAAGT+TGG + chr4_3:73110619-73110638 Msa0661620:three_prime_UTR 30.0%
! GTAAAAGTGTGGAAATCTAG+CGG + chr4_3:73107321-73107340 Msa0661620:five_prime_UTR 35.0%
ATCCCGAAAATCATAGTGAA+TGG + chr4_3:73107361-73107380 Msa0661620:exon 35.0%
! AGAAGAGAGGTTTGTTTGAA+GGG + chr4_3:73107392-73107411 Msa0661620:CDS 35.0%
! CAAAGTCGCTTTGCAATTTA+TGG + chr4_3:73107445-73107464 Msa0661620:CDS 35.0%
ATTTCATTGCCAACATTCAG+AGG + chr4_3:73107574-73107593 Msa0661620:intron 35.0%
GAAATTTCTCCTCTGAATGT+TGG - chr4_3:73107586-73107605 None:intergenic 35.0%
!! GCTGTTTTCATAAGCTATTC+TGG + chr4_3:73107642-73107661 Msa0661620:intron 35.0%
TAAGATATTCATCACAGGGA+TGG + chr4_3:73107772-73107791 Msa0661620:intron 35.0%
AGCTAGTGCTAGATATTCTA+TGG + chr4_3:73107803-73107822 Msa0661620:CDS 35.0%
GCTAGTGCTAGATATTCTAT+GGG + chr4_3:73107804-73107823 Msa0661620:CDS 35.0%
GCGCGTTAATAGTTCTTTAT+TGG + chr4_3:73107833-73107852 Msa0661620:CDS 35.0%
GAATCCTACTAGTATCATCT+TGG + chr4_3:73107948-73107967 Msa0661620:intron 35.0%
GTCAATGGGGAAAACATATT+TGG - chr4_3:73108224-73108243 None:intergenic 35.0%
ACCATAACAGAGAACTTAAC+AGG + chr4_3:73108465-73108484 Msa0661620:three_prime_UTR 35.0%
TCCTGTTAAGTTCTCTGTTA+TGG - chr4_3:73108469-73108488 None:intergenic 35.0%
GGAAGCTTCAAATGAGAAAT+TGG + chr4_3:73108653-73108672 Msa0661620:intron 35.0%
AGCTTCTCATAAAGTAGAGT+TGG + chr4_3:73108795-73108814 Msa0661620:intron 35.0%
CTCATAAAGTAGAGTTGGAT+TGG + chr4_3:73108800-73108819 Msa0661620:intron 35.0%
TAGTTCAGATGCTCATGTTA+AGG - chr4_3:73108891-73108910 None:intergenic 35.0%
! GTGGTTTTCATAGGTTCAAA+AGG + chr4_3:73108937-73108956 Msa0661620:intron 35.0%
CAAAACTCCAAAAACTGACA+AGG - chr4_3:73108975-73108994 None:intergenic 35.0%
ACCTTTCTCAAATGACAGTT+GGG - chr4_3:73109020-73109039 None:intergenic 35.0%
ATGAGGTTATCTATACCTGA+AGG - chr4_3:73109261-73109280 None:intergenic 35.0%
ATGAGTAATGTTATGAGGGA+CGG + chr4_3:73109747-73109766 Msa0661620:intron 35.0%
TGAGCTAAGAGAAAAGGAAT+TGG - chr4_3:73109871-73109890 None:intergenic 35.0%
! CTGATTTGAGCTAAGAGAAA+AGG - chr4_3:73109877-73109896 None:intergenic 35.0%
ACATTCACTTAGCATTTAGG+GGG - chr4_3:73109913-73109932 None:intergenic 35.0%
TGTAAAATGAGAAGCGAATG+GGG - chr4_3:73110093-73110112 None:intergenic 35.0%
CTTGTAAAATGAGAAGCGAA+TGG - chr4_3:73110095-73110114 None:intergenic 35.0%
AGAAAAGCCATATTGTGATC+GGG - chr4_3:73110121-73110140 None:intergenic 35.0%
!! CAAGAGTATCGATCACAATA+TGG + chr4_3:73110159-73110178 Msa0661620:intron 35.0%
! CAAGGAAAGATCAAAGGTTT+TGG - chr4_3:73110257-73110276 None:intergenic 35.0%
!!! GAGTATTTGTTTTATCACGC+TGG + chr4_3:73110392-73110411 Msa0661620:intron 35.0%
! ACACCGAGAAACTTTTCATT+TGG - chr4_3:73110526-73110545 None:intergenic 35.0%
AATGGAAGAAGAGAGAAGAG+AGG + chr4_3:73107379-73107398 Msa0661620:exon 40.0%
! GAGAAGAGAGGTTTGTTTGA+AGG + chr4_3:73107391-73107410 Msa0661620:CDS 40.0%
AAGCTATTCTGGTGAGCTTA+TGG + chr4_3:73107653-73107672 Msa0661620:intron 40.0%
GCTGAAAACAACTTATGGTG+AGG + chr4_3:73107682-73107701 Msa0661620:intron 40.0%
TAGCAGCTGAATGGAATTTG+AGG - chr4_3:73107860-73107879 None:intergenic 40.0%
TCAATGTTGTAGCAGCTGAA+TGG - chr4_3:73107869-73107888 None:intergenic 40.0%
CATGCCAAGATGATACTAGT+AGG - chr4_3:73107955-73107974 None:intergenic 40.0%
ACCATTGCACTCGATTCAAT+AGG + chr4_3:73108060-73108079 Msa0661620:intron 40.0%
ACCTATTGAATCGAGTGCAA+TGG - chr4_3:73108064-73108083 None:intergenic 40.0%
ACACTTCACGTTGCAGAAAT+GGG + chr4_3:73108092-73108111 Msa0661620:CDS 40.0%
!!! CTTGGTTTTGCAAACTACTG+AGG + chr4_3:73108440-73108459 Msa0661620:three_prime_UTR 40.0%
TTTGCTCTCATGGATTCACA+GGG + chr4_3:73108835-73108854 Msa0661620:intron 40.0%
! CACAAGTCCTTGTCAGTTTT+TGG + chr4_3:73108965-73108984 Msa0661620:three_prime_UTR 40.0%
! ACCCAACTGTCATTTGAGAA+AGG + chr4_3:73109016-73109035 Msa0661620:three_prime_UTR 40.0%
CACCTTTCTCAAATGACAGT+TGG - chr4_3:73109021-73109040 None:intergenic 40.0%
! CAACTGTCATTTGAGAAAGG+TGG + chr4_3:73109019-73109038 Msa0661620:intron 40.0%
TAACCAACTTCATCAAGAGG+TGG + chr4_3:73109206-73109225 Msa0661620:three_prime_UTR 40.0%
TGAAGAAGTAGCAATCCTTC+AGG + chr4_3:73109243-73109262 Msa0661620:three_prime_UTR 40.0%
GCATGCCATCGTATAACATA+AGG + chr4_3:73109560-73109579 Msa0661620:intron 40.0%
TCCAGTTTCAAACACCATGA+GGG + chr4_3:73109685-73109704 Msa0661620:intron 40.0%
GCTAAGAGAAAAGGAATTGG+AGG - chr4_3:73109868-73109887 None:intergenic 40.0%
GTAAAATGAGAAGCGAATGG+GGG - chr4_3:73110092-73110111 None:intergenic 40.0%
ATTCAAGGTCTGTTTGGACT+GGG - chr4_3:73110215-73110234 None:intergenic 40.0%
AATTCAAGGTCTGTTTGGAC+TGG - chr4_3:73110216-73110235 None:intergenic 40.0%
TAGGAGCAAGGAAAGATCAA+AGG - chr4_3:73110263-73110282 None:intergenic 40.0%
CACCAAAATCTTGCAATGCT+CGG - chr4_3:73110482-73110501 None:intergenic 40.0%
AAGTGTGGAGGAGAGAGAAT+CGG - chr4_3:73107498-73107517 None:intergenic 45.0%
TGTATGTACCTGTCGAAGTG+TGG - chr4_3:73107513-73107532 None:intergenic 45.0%
ATATTCATCACAGGGATGGC+TGG + chr4_3:73107776-73107795 Msa0661620:intron 45.0%
CATTTCTGCAACGTGAAGTG+TGG - chr4_3:73108093-73108112 None:intergenic 45.0%
CACACTTCACGTTGCAGAAA+TGG + chr4_3:73108091-73108110 Msa0661620:CDS 45.0%
!! ACTTTCAGGTTCGTGTTCAG+AGG - chr4_3:73108121-73108140 None:intergenic 45.0%
!! ATGTTTTCCCCATTGACTGC+TGG + chr4_3:73108227-73108246 Msa0661620:exon 45.0%
TATCGATGCCAGCAGTCAAT+GGG - chr4_3:73108238-73108257 None:intergenic 45.0%
!! TGCTGGCATCGATAGTGTAA+AGG + chr4_3:73108244-73108263 Msa0661620:three_prime_UTR 45.0%
!! GCTGGCATCGATAGTGTAAA+GGG + chr4_3:73108245-73108264 Msa0661620:three_prime_UTR 45.0%
!! CTGGCATCGATAGTGTAAAG+GGG + chr4_3:73108246-73108265 Msa0661620:three_prime_UTR 45.0%
!! TGGCATCGATAGTGTAAAGG+GGG + chr4_3:73108247-73108266 Msa0661620:three_prime_UTR 45.0%
CTTTGCTCTCATGGATTCAC+AGG + chr4_3:73108834-73108853 Msa0661620:intron 45.0%
CTTCCACCTCTTGATGAAGT+TGG - chr4_3:73109212-73109231 None:intergenic 45.0%
GACAAGTATCTCACACACTC+TGG - chr4_3:73109434-73109453 None:intergenic 45.0%
CTCCAGTTTCAAACACCATG+AGG + chr4_3:73109684-73109703 Msa0661620:intron 45.0%
! ACCCTCATGGTGTTTGAAAC+TGG - chr4_3:73109689-73109708 None:intergenic 45.0%
! TTCAAACACCATGAGGGTGT+TGG + chr4_3:73109691-73109710 Msa0661620:intron 45.0%
ATTTGACACCAACACCCTCA+TGG - chr4_3:73109702-73109721 None:intergenic 45.0%
GAAAAGGAATTGGAGGTCCT+GGG - chr4_3:73109861-73109880 None:intergenic 45.0%
AGAAAAGGAATTGGAGGTCC+TGG - chr4_3:73109862-73109881 None:intergenic 45.0%
CACTTAGCATTTAGGGGGTT+GGG - chr4_3:73109908-73109927 None:intergenic 45.0%
TCACTTAGCATTTAGGGGGT+TGG - chr4_3:73109909-73109928 None:intergenic 45.0%
CAAGAGTCCCGATCACAATA+TGG + chr4_3:73110111-73110130 Msa0661620:intron 45.0%
TTCAAGGTCTGTTTGGACTG+GGG - chr4_3:73110214-73110233 None:intergenic 45.0%
!! AAGGTTTTGGCCCCAATTAC+AGG - chr4_3:73110244-73110263 None:intergenic 45.0%
! TTTTATTCAGGCTGCCAGAG+CGG + chr4_3:73110431-73110450 Msa0661620:intron 45.0%
!! GACCGAGCATTGCAAGATTT+TGG + chr4_3:73110477-73110496 Msa0661620:three_prime_UTR 45.0%
GGAGGAGAGAGAATCGGAAA+GGG - chr4_3:73107492-73107511 None:intergenic 50.0%
TGGAGGAGAGAGAATCGGAA+AGG - chr4_3:73107493-73107512 None:intergenic 50.0%
ATGTACCTGTCGAAGTGTGG+AGG - chr4_3:73107510-73107529 None:intergenic 50.0%
AGACAAGAATGAGCAGCCAC+AGG + chr4_3:73108152-73108171 Msa0661620:CDS 50.0%
ATCGATGCCAGCAGTCAATG+GGG - chr4_3:73108237-73108256 None:intergenic 50.0%
CTATCGATGCCAGCAGTCAA+TGG - chr4_3:73108239-73108258 None:intergenic 50.0%
GTGAGATACTTGTCTCGCTG+TGG + chr4_3:73109440-73109459 Msa0661620:intron 50.0%
TCATCGTTTGCCATCCACTG+TGG - chr4_3:73109731-73109750 None:intergenic 50.0%
! TCTGTTTGGACTGGGGGTTT+AGG - chr4_3:73110207-73110226 None:intergenic 50.0%
! TCAAGGTCTGTTTGGACTGG+GGG - chr4_3:73110213-73110232 None:intergenic 50.0%
TCCTTGCTCCTAAAGCTGAG+AGG + chr4_3:73110271-73110290 Msa0661620:intron 50.0%
!! TCCTCTCAGCTTTAGGAGCA+AGG - chr4_3:73110275-73110294 None:intergenic 50.0%
CTGTCTCTCCTCTCAGCTTT+AGG - chr4_3:73110282-73110301 None:intergenic 50.0%
TCTCTCCTCCACACTTCGAC+AGG + chr4_3:73107502-73107521 Msa0661620:CDS 55.0%
TTGGGTCGCACGAAGCTTTG+GGG - chr4_3:73109002-73109021 None:intergenic 55.0%
GTTGGGTCGCACGAAGCTTT+GGG - chr4_3:73109003-73109022 None:intergenic 55.0%
AGTTGGGTCGCACGAAGCTT+TGG - chr4_3:73109004-73109023 None:intergenic 55.0%
CTGGGTTCAAACCCAGAGGA+AGG - chr4_3:73109843-73109862 None:intergenic 55.0%
CTTCCTCTGGGTTTGAACCC+AGG + chr4_3:73109841-73109860 Msa0661620:intron 55.0%
TGCCAGAGCGGCATCACAAA+TGG + chr4_3:73110443-73110462 Msa0661620:three_prime_UTR 55.0%
CTCCATTTGTGATGCCGCTC+TGG - chr4_3:73110448-73110467 None:intergenic 55.0%
GGTCCTGGGTTCAAACCCAG+AGG - chr4_3:73109847-73109866 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 73107321 73110701 73107321 ID=Msa0661620;Name=Msa0661620
chr4_3 mRNA 73107321 73110701 73107321 ID=Msa0661620-mRNA-1;Parent=Msa0661620;Name=Msa0661620-mRNA-1;_AED=0.05;_eAED=0.05;_QI=59|1|1|1|0.4|0.33|6|727|141
chr4_3 exon 73107321 73107523 73107321 ID=Msa0661620-mRNA-1:exon:22004;Parent=Msa0661620-mRNA-1
chr4_3 exon 73107789 73107909 73107789 ID=Msa0661620-mRNA-1:exon:22005;Parent=Msa0661620-mRNA-1
chr4_3 exon 73108082 73108506 73108082 ID=Msa0661620-mRNA-1:exon:22006;Parent=Msa0661620-mRNA-1
chr4_3 exon 73108950 73109037 73108950 ID=Msa0661620-mRNA-1:exon:22007;Parent=Msa0661620-mRNA-1
chr4_3 exon 73109151 73109264 73109151 ID=Msa0661620-mRNA-1:exon:22008;Parent=Msa0661620-mRNA-1
chr4_3 exon 73110441 73110701 73110441 ID=Msa0661620-mRNA-1:exon:22009;Parent=Msa0661620-mRNA-1
chr4_3 five_prime_UTR 73107321 73107379 73107321 ID=Msa0661620-mRNA-1:five_prime_utr;Parent=Msa0661620-mRNA-1
chr4_3 CDS 73107380 73107523 73107380 ID=Msa0661620-mRNA-1:cds;Parent=Msa0661620-mRNA-1
chr4_3 CDS 73107789 73107909 73107789 ID=Msa0661620-mRNA-1:cds;Parent=Msa0661620-mRNA-1
chr4_3 CDS 73108082 73108242 73108082 ID=Msa0661620-mRNA-1:cds;Parent=Msa0661620-mRNA-1
chr4_3 three_prime_UTR 73108243 73108506 73108243 ID=Msa0661620-mRNA-1:three_prime_utr;Parent=Msa0661620-mRNA-1
chr4_3 three_prime_UTR 73108950 73109037 73108950 ID=Msa0661620-mRNA-1:three_prime_utr;Parent=Msa0661620-mRNA-1
chr4_3 three_prime_UTR 73109151 73109264 73109151 ID=Msa0661620-mRNA-1:three_prime_utr;Parent=Msa0661620-mRNA-1
chr4_3 three_prime_UTR 73110441 73110701 73110441 ID=Msa0661620-mRNA-1:three_prime_utr;Parent=Msa0661620-mRNA-1
Gene Sequence

>Msa0661620

ATGGAAGAAGAGAGAAGAGAGGTTTGTTTGAAGGGTGAAGAAGAAGTTGTTATTAAAGATGAAGACAAAGTCGCTTTGCAATTTATGGATTCACTTCATAACTATCTATCCCTTTCCGATTCTCTCTCCTCCACACTTCGACAGGGATGGCTGGAGTTAGCTAGTGCTAGATATTCTATGGGTACTTCGCGCGTTAATAGTTCTTTATTGGACCTCAAATTCCATTCAGCTGCTACAACATTGAAGATATCCGAAAATCAAGATGGTACACAACCACACTTCACGTTGCAGAAATGGGTATCCTCTGAACACGAACCTGAAAGTACTAAATTAGAAGACAAGAATGAGCAGCCACAGGATAGTAATAGTATGAAATCATCTGGTACACTTTTCTTCTCTCAATACCAAATATGTTTTCCCCATTGA
Protein sequence

>Msa0661620

MEEERREVCLKGEEEVVIKDEDKVALQFMDSLHNYLSLSDSLSSTLRQGWLELASARYSMGTSRVNSSLLDLKFHSAATTLKISENQDGTQPHFTLQKWVSSEHEPESTKLEDKNEQPQDSNSMKSSGTLFFSQYQICFPH*