AlfalfaGEDB | Msa0678040

M. sativa cultivar zhongmu-4 / Msa0678040

KEGG: K09422
GO: -
PFAM: PF00249.34

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0678040	XP_013443536.1	90.511	274	19	2	1	273	1	268	1.44e-171	485

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0678040	sp\|Q9SJX8\|MYB14_ARATH	48.241	199	89	1	1	199	1	185	1.56e-58	190

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0678040	A0A072TK55	90.511	274	19	2	1	273	1	268	6.87e-172	485

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TFs/TRs:

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Gene ID	Type	Classification
Msa0678040	TF	MYB

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0030160	Msa0678040	0.812688	3.737476e-51	-8.615850e-47
Msa0101240	Msa0678040	0.820595	6.311439e-53	-8.615850e-47
Msa0259440	Msa0678040	0.815702	8.073179e-52	-8.615850e-47
Msa0259580	Msa0678040	0.814154	1.779930e-51	-8.615850e-47
Msa0543130	Msa0678040	0.814465	1.519916e-51	-8.615850e-47
Msa0543140	Msa0678040	0.862586	4.563363e-64	-8.615850e-47
Msa0599140	Msa0678040	0.885356	8.724841e-72	-8.615850e-47
Msa0599150	Msa0678040	0.865865	4.330054e-65	-8.615850e-47
Msa0678040	Msa0715420	0.841840	3.722826e-58	-8.615850e-47
Msa0678040	Msa0772710	0.802564	5.284641e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0678040	MtrunA17_Chr4g0022901	90.511	274	19	2	1	273	1	268	1.32e-175	485
Msa0678040	MtrunA17_Chr4g0022911	77.133	293	39	4	1	272	1	286	8.76e-154	431
Msa0678040	MtrunA17_Chr4g0022931	74.909	275	58	6	1	272	1	267	1.99e-133	378
Msa0678040	MtrunA17_Chr2g0323351	46.715	274	112	7	1	272	1	242	2.53e-75	230
Msa0678040	MtrunA17_Chr1g0187761	48.000	200	93	3	1	199	1	190	4.49e-58	186
Msa0678040	MtrunA17_Chr1g0167661	42.292	253	121	8	1	242	1	239	4.95e-58	186
Msa0678040	MtrunA17_Chr7g0274581	48.421	190	85	2	1	180	1	187	1.00e-57	183
Msa0678040	MtrunA17_Chr1g0167691	46.734	199	94	2	1	199	1	187	1.69e-57	184
Msa0678040	MtrunA17_Chr7g0261281	67.227	119	39	0	1	119	1	119	3.44e-56	182
Msa0678040	MtrunA17_Chr4g0022881	63.158	114	42	0	1	114	1	114	2.21e-54	176

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0678040	AT2G31180.1	48.241	199	89	1	1	199	1	185	1.59e-59	190
Msa0678040	AT3G23250.1	45.536	224	101	6	1	203	1	224	8.48e-59	189
Msa0678040	AT1G06180.1	47.739	199	90	3	1	199	1	185	4.88e-57	183
Msa0678040	AT4G28110.1	64.957	117	41	0	1	117	1	117	1.18e-55	181
Msa0678040	AT4G38620.1	51.299	154	73	1	1	154	1	152	2.66e-54	177
Msa0678040	AT3G02940.2	62.393	117	44	0	1	117	1	117	1.46e-53	176
Msa0678040	AT4G21440.1	62.393	117	44	0	1	117	1	117	1.98e-53	177
Msa0678040	AT5G16770.4	61.538	117	45	0	1	117	1	117	3.03e-53	176
Msa0678040	AT5G16770.3	61.538	117	45	0	1	117	1	117	3.03e-53	176
Msa0678040	AT3G02940.1	62.393	117	44	0	1	117	1	117	3.28e-53	176

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Find 57 sgRNAs with CRISPR-Local

Find 158 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACTTTACTCAACAAGAAG+AGG	0.563987	4_4:-32828460	Msa0678040:CDS
AAAGGTATCAGTAGTTCAGT+TGG	0.561036	4_4:+32827907	None:intergenic
AAATGGGGATAAAGGGCCAT+TGG	0.578413	4_4:+32828197	None:intergenic
AACCATTGCGGCTGAGTTAC+CGG	0.309509	4_4:-32828394	Msa0678040:CDS
AACTGGATTGAGGAGTAATT+TGG	0.303507	4_4:+32828235	None:intergenic
AATCTTTCCAACACATCAAG+TGG	0.540288	4_4:-32828060	Msa0678040:CDS
ACATAATTGAATCCACACAA+GGG	0.713140	4_4:-32828275	Msa0678040:CDS
ACCAAAATCATCCAAAGAAG+TGG	0.496803	4_4:+32828131	None:intergenic
ACCATTGCGGCTGAGTTACC+GGG	0.548881	4_4:-32828393	Msa0678040:CDS
ACTACTGATACCTTTGTCGA+TGG	0.606426	4_4:-32827899	Msa0678040:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATTGCTTATGTCACTAGATA+TGG	-	chr4_4:32827921-32827940	Msa0678040:CDS	30.0%
CCTCCTTGAGATAAAATATA+TGG	-	chr4_4:32828026-32828045	Msa0678040:CDS	30.0%
AAACTTTACTCAACAAGAAG+AGG	-	chr4_4:32828164-32828183	Msa0678040:CDS	30.0%
ATCAGAATGCATAAAAAGCT+TGG	-	chr4_4:32828195-32828214	Msa0678040:CDS	30.0%
TCAGAATGCATAAAAAGCTT+GGG	-	chr4_4:32828196-32828215	Msa0678040:CDS	30.0%
ATAAAAAGCTTGGGAATAGA+TGG	-	chr4_4:32828205-32828224	Msa0678040:CDS	30.0%
ATAATGAAGTGAAGAACCAT+TGG	-	chr4_4:32828262-32828281	Msa0678040:CDS	30.0%
ACATAATTGAATCCACACAA+GGG	-	chr4_4:32828349-32828368	Msa0678040:CDS	30.0%
GATAATGAAAATGGGGATAA+AGG	+	chr4_4:32828438-32828457	None:intergenic	30.0%
ATTGCTTATGTCACTAGATA+TGG	-	chr4_4:32827921-32827940	Msa0678040:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_4	gene	32827846	32828800	32827846	ID=Msa0678040;Name=Msa0678040
chr4_4	mRNA	32827846	32828800	32827846	ID=Msa0678040-mRNA-1;Parent=Msa0678040;Name=Msa0678040-mRNA-1;_AED=0.05;_eAED=0.05;_QI=0\|0\|0\|1\|1\|1\|2\|0\|279
chr4_4	exon	32828668	32828800	32828668	ID=Msa0678040-mRNA-1:exon:8073;Parent=Msa0678040-mRNA-1
chr4_4	exon	32827846	32828552	32827846	ID=Msa0678040-mRNA-1:exon:8072;Parent=Msa0678040-mRNA-1
chr4_4	CDS	32828668	32828800	32828668	ID=Msa0678040-mRNA-1:cds;Parent=Msa0678040-mRNA-1
chr4_4	CDS	32827846	32828552	32827846	ID=Msa0678040-mRNA-1:cds;Parent=Msa0678040-mRNA-1

Gene Sequence

>Msa0678040

ATGGCAAGAACTCCTTCTTGTGACAAAAGTGGAATGAGGAAAGGAACATGGACTGCTGAAGAAGATAGGAAATTAATTGCTTATGTCACTAGATATGGTTGCTGGAATTGGCGACAACTCCCTAAGTTTGTAGGTCTTTCAAGGTGTGGGAAGAGTTGTAGACTTAGGTGGCTGAATTATCTAAGGCCTAATATCAAAAGAGGAAACTTTACTCAACAAGAAGAGGAGTTGATTATCAGAATGCATAAAAAGCTTGGGAATAGATGGTCAACCATTGCGGCTGAGTTACCGGGAAGAACAGATAATGAAGTGAAGAACCATTGGCACACTTCTCTCAAGAAGAGAGCTATAGATAACATAGTCACGAATGAAGAAACGAAATCAAAAGACATAATTGAATCCACACAAGGGAGAGATATATCCAATTACCAAATTACTCCTCAATCCAGTTCCCAAATCTCAGACACCAATGGCCCTTTATCCCCATTTTCATTATCTAGTGAAATCACTTCCACATCTTTAGATCACAATTCCACTTCTTTGGATGATTTTGGTTTTCTAGATTCATTTATAGAAGATGTGGATGAAAGTTTTTGGCTTCATAATCTTTCCAACACATCAAGTGGAACAGTGCAAAACCATACTACCGAAAGTTTTGTCATGGATAATGATTTTGGAAGCTTTTTAGATGCTTACAAGGTGTCAACAGTTGATAGTTTTTGGACACAACCATATGAGGTTGACATGTCCCTCGTTCCAACTGAACTACTGATACCTTTGTCGATGGAATCTGAAAATTTTTCTATAGTATATGACAAGATATATTTGGAGTTGAAATAA

Protein sequence

>Msa0678040

MARTPSCDKSGMRKGTWTAEEDRKLIAYVTRYGCWNWRQLPKFVGLSRCGKSCRLRWLNYLRPNIKRGNFTQQEEELIIRMHKKLGNRWSTIAAELPGRTDNEVKNHWHTSLKKRAIDNIVTNEETKSKDIIESTQGRDISNYQITPQSSSQISDTNGPLSPFSLSSEITSTSLDHNSTSLDDFGFLDSFIEDVDESFWLHNLSNTSSGTVQNHTTESFVMDNDFGSFLDAYKVSTVDSFWTQPYEVDMSLVPTELLIPLSMESENFSIVYDKIYLELK*