AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0772710

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0772710 MtrunA17_Chr5g0402701 92.929 198 13 1 26 223 1 197 1.54e-119 356
Msa0772710 MtrunA17_Chr5g0402701 89.247 186 18 1 226 409 287 472 3.23e-110 332
Msa0772710 MtrunA17_Chr5g0402811 71.123 187 28 2 223 409 4 164 3.67e-87 264
Msa0772710 MtrunA17_Chr5g0402691 90.083 121 10 1 275 393 273 393 7.35e-66 216
Msa0772710 MtrunA17_Chr5g0402671 46.729 214 93 3 26 223 1 209 4.03e-42 158
Msa0772710 MtrunA17_Chr5g0402771 45.673 208 102 2 26 223 1 207 8.25e-42 157
Msa0772710 MtrunA17_Chr5g0402721 46.766 201 97 5 26 223 1 194 4.27e-37 143
Msa0772710 MtrunA17_Chr5g0402681 44.500 200 102 5 26 223 1 193 7.32e-33 131
Msa0772710 MtrunA17_Chr5g0402761 38.813 219 106 4 26 223 1 212 4.41e-32 123
Msa0772710 MtrunA17_Chr8g0387631 37.795 127 79 0 283 409 79 205 5.43e-21 91.7
Msa0772710 MtrunA17_Chr8g0374551 30.348 201 133 5 27 223 3 200 6.50e-18 86.3
Msa0772710 MtrunA17_Chr8g0348131 37.302 126 79 0 283 408 83 208 1.73e-17 82.0
Msa0772710 MtrunA17_Chr1g0202401 33.858 127 84 0 282 408 71 197 2.72e-17 80.9
Msa0772710 MtrunA17_Chr8g0374561 32.843 204 128 6 27 222 3 205 9.92e-16 79.3
Msa0772710 MtrunA17_Chr3g0095441 30.827 133 92 0 277 409 58 190 3.18e-14 72.0
Msa0772710 MtrunA17_Chr6g0453331 32.331 133 90 0 278 410 13 145 3.98e-13 67.8
Msa0772710 MtrunA17_Chr5g0403281 49.254 67 34 0 26 92 1 67 5.15e-13 64.3
Msa0772710 MtrunA17_Chr5g0442361 34.815 135 86 1 277 409 57 191 5.85e-13 68.2
Msa0772710 MtrunA17_Chr8g0376101 26.316 133 98 0 277 409 36 168 4.60e-12 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0772710 AT2G23640.1 35.616 146 83 1 264 409 13 147 1.80e-25 103
Msa0772710 AT5G41600.1 33.594 128 85 0 283 410 73 200 6.28e-19 86.3
Msa0772710 AT3G61560.3 35.714 126 81 0 283 408 73 198 2.25e-18 83.6
Msa0772710 AT3G61560.1 35.714 126 81 0 283 408 73 198 2.56e-18 84.3
Msa0772710 AT3G61560.2 35.714 126 81 0 283 408 73 198 2.56e-18 84.3
Msa0772710 AT1G64090.1 35.938 128 82 0 283 410 69 196 5.86e-18 83.6
Msa0772710 AT1G64090.2 35.938 128 82 0 283 410 85 212 6.68e-18 83.6
Msa0772710 AT4G23630.1 33.071 127 85 0 283 409 94 220 6.90e-18 83.6
Msa0772710 AT4G23630.2 33.071 127 85 0 283 409 94 220 6.90e-18 83.6
Msa0772710 AT2G46170.1 34.921 126 82 0 283 408 73 198 3.08e-17 81.3
Msa0772710 AT4G11220.1 32.283 127 86 0 283 409 90 216 4.83e-17 81.3
Msa0772710 AT3G10260.1 27.879 165 110 1 254 409 28 192 9.66e-14 71.2
Msa0772710 AT3G10260.2 27.879 165 110 1 254 409 28 192 9.66e-14 71.2
Msa0772710 AT3G10260.3 27.879 165 110 1 254 409 48 212 1.44e-13 70.9
Msa0772710 AT3G19460.1 30.233 129 90 0 282 410 26 154 2.16e-13 68.9
Msa0772710 AT3G19460.5 30.233 129 90 0 282 410 26 154 2.16e-13 68.9
Msa0772710 AT3G19460.4 30.233 129 90 0 282 410 26 154 2.16e-13 68.9
Msa0772710 AT2G46170.2 32.692 104 70 0 283 386 73 176 5.98e-12 64.7

Find 100 sgRNAs with CRISPR-Local

Find 210 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCCACCATTTAAACTAAAA+TGG 0.148717 5_2:-47060079 None:intergenic
TCTTCAAATGAATCTTTCTT+TGG 0.231488 5_2:+47058056 Msa0772710:CDS
TTGTTCCATTTGAGAATAAA+AGG 0.254108 5_2:+47057842 Msa0772710:CDS
ATGGGAATCAAATAAGGTTT+TGG 0.296861 5_2:+47057902 Msa0772710:CDS
ACTTCCTCACTCTCATTTCA+TGG 0.305776 5_2:+47059295 Msa0772710:CDS
CATTATTACTTGCATAGTTA+TGG 0.328058 5_2:+47057480 Msa0772710:CDS
TTGCAATGCTTTGGCTCCTC+TGG 0.328181 5_2:+47057621 Msa0772710:CDS
GTGGAATCAAACAAATGTTT+TGG 0.331082 5_2:-47057442 None:intergenic
AGATTCATGGGAATCAAATA+AGG 0.331240 5_2:+47057896 Msa0772710:CDS
GTTGGATAATAAATTTGATT+TGG 0.341168 5_2:+47060030 Msa0772710:three_prime_UTR
AATTCAAGCCTCAACAAGTT+TGG 0.347496 5_2:-47059659 None:intergenic
GAACTCAAGTTATTTATAGA+TGG 0.356153 5_2:-47057401 None:intergenic
TGAAGCTTTGCTTGTTGAGC+TGG 0.364796 5_2:-47057781 None:intergenic
CCTCTGTTGAATGCTTGAAT+TGG 0.373089 5_2:-47057559 None:intergenic
AACTAAGTACAAGTATTCTA+TGG 0.379368 5_2:+47059196 Msa0772710:CDS
GCCGAAAAGTGTAAGCATAT+TGG 0.387337 5_2:-47059355 None:intergenic
TTCCTAACATTTATTTATAT+AGG 0.392131 5_2:+47059842 Msa0772710:CDS
CTTGAGATGATACTTTAATT+CGG 0.398518 5_2:-47060309 None:intergenic
CTAACACTTCATTAGTTCAA+TGG 0.403190 5_2:-47060054 None:intergenic
TTCCAAATACCTTGGATTTG+TGG 0.404601 5_2:-47058081 None:intergenic
TCAAGCCTTGGAGTAAATTC+AGG 0.412808 5_2:-47057946 None:intergenic
GAATGAGAGGGAGTTGAGAA+AGG 0.414282 5_2:-47059054 None:intergenic
GCATGTGTTTGTGGGAGTTA+TGG 0.420480 5_2:+47059778 Msa0772710:CDS
GTTCTTGCGGAAAAGATTCA+TGG 0.421247 5_2:+47057883 Msa0772710:CDS
TACCTATATAAATAAATGTT+AGG 0.429849 5_2:-47059844 None:intergenic
AGAAAGATTCATTTGAAGAG+TGG 0.429858 5_2:-47058052 None:intergenic
TCAACTCCCTCTCATTCATC+AGG 0.436068 5_2:+47059060 Msa0772710:CDS
GAAAGATTCATTTGAAGAGT+GGG 0.443477 5_2:-47058051 None:intergenic
GGTATGACCCTTCCTGCAAA+TGG 0.453078 5_2:+47059969 Msa0772710:three_prime_UTR
TGTTGAAGGACATGGTACTA+TGG 0.458002 5_2:+47059163 Msa0772710:CDS
GTGTAAGCATATTGGAATAG+AGG 0.464032 5_2:-47059347 None:intergenic
CTGCCTCTTTCGAGAGTGGA+AGG 0.465383 5_2:-47057592 None:intergenic
CTCTGTTGAATGCTTGAATT+GGG 0.471195 5_2:-47057558 None:intergenic
ATCAATAAGGTGGTTGTTCG+TGG 0.471872 5_2:+47059736 Msa0772710:CDS
AGATGCATGGAGTGGTTTGT+TGG 0.476896 5_2:+47060012 Msa0772710:three_prime_UTR
AATAGTTTGCAACGATTCGT+TGG 0.477214 5_2:+47057654 Msa0772710:CDS
AGAAACTTGACGCTCTTCAC+TGG 0.480016 5_2:+47057806 Msa0772710:CDS
GGGAGTTATGGCTACGTTGT+TGG 0.486141 5_2:+47059790 Msa0772710:CDS
GAAAGATTGGCATGTGTTTG+TGG 0.488969 5_2:+47059769 Msa0772710:CDS
AAGATTGAGTTGATCAAGAA+TGG 0.492616 5_2:+47057985 Msa0772710:CDS
AACAGCAACATGGTTGTTGA+TGG 0.492674 5_2:+47059257 Msa0772710:CDS
AATGTAGGCAAAGGTAAAGT+GGG 0.497067 5_2:+47057350 Msa0772710:five_prime_UTR
CTCTTATGTAGGAGCCTGCA+TGG 0.498378 5_2:+47059817 Msa0772710:CDS
AAGGGATTTGATGAAGCTGT+TGG 0.504716 5_2:+47057861 Msa0772710:CDS
AGCTGTCGTTGGAGCTGTGG+TGG 0.506052 5_2:+47057519 Msa0772710:CDS
AAATGTAGGCAAAGGTAAAG+TGG 0.507798 5_2:+47057349 Msa0772710:five_prime_UTR
ATAGTTTGCAACGATTCGTT+GGG 0.509757 5_2:+47057655 Msa0772710:CDS
TGGTAAGCATAAAGAAAGAT+TGG 0.509969 5_2:+47059756 Msa0772710:CDS
AGAAGAAACAGCTGTGAGAA+TGG 0.511563 5_2:+47059685 Msa0772710:CDS
AAAGATTGGCATGTGTTTGT+GGG 0.514124 5_2:+47059770 Msa0772710:CDS
CAATCTTCAACTCAGTGAAA+AGG 0.517566 5_2:-47057969 None:intergenic
TGTTCAATAGGTATAGTAGC+TGG 0.519195 5_2:+47059948 Msa0772710:three_prime_UTR
TGTTCCATTTGAGAATAAAA+GGG 0.520222 5_2:+47057843 Msa0772710:CDS
TACCCTTCCACTCTCGAAAG+AGG 0.522302 5_2:+47057589 Msa0772710:CDS
TCAAGCCTCAACAAGTTTGG+TGG 0.523210 5_2:-47059656 None:intergenic
AAGAAACTACAAGGAAATGT+AGG 0.524795 5_2:+47057335 None:intergenic
GACAAACAGTTCTTGAAAGT+AGG 0.527060 5_2:-47057754 None:intergenic
TTGTTGGTAATCTCTTATGT+AGG 0.529651 5_2:+47059806 Msa0772710:CDS
CGTTGGAGCTGTGGTGGGAG+AGG 0.529725 5_2:+47057525 Msa0772710:CDS
TAGTACTATTGTTCTAATTG+CGG 0.541056 5_2:+47059233 Msa0772710:CDS
ATTAATACAACCAATTCGAG+AGG 0.544615 5_2:-47059912 None:intergenic
CTAGTGAGTGGAGCTGTCGT+TGG 0.549910 5_2:+47057508 Msa0772710:CDS
ACTAATTCTGTATCTTCACT+TGG 0.555941 5_2:-47057682 None:intergenic
AAATGTTAGGAAGTCCATGC+AGG 0.559443 5_2:-47059831 None:intergenic
TTCTTGCGGAAAAGATTCAT+GGG 0.561706 5_2:+47057884 Msa0772710:CDS
GTACAAGTATTCTATGGAGG+AGG 0.565591 5_2:+47059202 Msa0772710:CDS
TGATGGCAATGACATGTTGA+AGG 0.566160 5_2:+47059149 Msa0772710:CDS
TCAGTGAAAAGGTCAAGCCT+TGG 0.568065 5_2:-47057958 None:intergenic
ATAATTACCTGATGAATGAG+AGG 0.575714 5_2:-47059067 None:intergenic
TGGCAAAGACAGTTCGAGCA+TGG 0.575869 5_2:+47059705 Msa0772710:CDS
GGGAACCACCAAACTTGTTG+AGG 0.576992 5_2:+47059651 Msa0772710:intron
TCTTTGGATCCACAAATCCA+AGG 0.578168 5_2:+47058072 Msa0772710:CDS
TATTACTTGCATAGTTATGG+CGG 0.585172 5_2:+47057483 Msa0772710:CDS
GAATTAGTAGAGACTATCAC+AGG 0.590087 5_2:+47057697 Msa0772710:CDS
TGCCTCTTTCGAGAGTGGAA+GGG 0.592328 5_2:-47057591 None:intergenic
GGTGGCAAGAACATAATGAC+AGG 0.597015 5_2:+47059105 Msa0772710:CDS
GCTGTCGTTGGAGCTGTGGT+GGG 0.600299 5_2:+47057520 Msa0772710:CDS
GATGAAGCTGTTGGTTCTTG+CGG 0.600796 5_2:+47057870 Msa0772710:CDS
TGGAGCTGTCGTTGGAGCTG+TGG 0.601920 5_2:+47057516 Msa0772710:CDS
CTACAAGGAAATGTAGGCAA+AGG 0.605410 5_2:+47057341 None:intergenic
GGATAAGATCAAGAGATGCA+TGG 0.608295 5_2:+47059999 Msa0772710:three_prime_UTR
AAGTTCCTGAATTTACTCCA+AGG 0.608416 5_2:+47057941 Msa0772710:CDS
AGGACATGGTACTATGGAGG+AGG 0.613160 5_2:+47059169 Msa0772710:CDS
TTAATACAACCAATTCGAGA+GGG 0.613461 5_2:-47059911 None:intergenic
GATGATACTTTAATTCGGAG+AGG 0.622366 5_2:-47060304 None:intergenic
TGAGCCATGAAATGAGAGTG+AGG 0.624540 5_2:-47059299 None:intergenic
GGAGTAAATTCAGGAACTTG+AGG 0.628397 5_2:-47057937 None:intergenic
TAATTGCGGCAACAGCAACA+TGG 0.630959 5_2:+47059247 Msa0772710:CDS
CAATGACATGTTGAAGGACA+TGG 0.631115 5_2:+47059155 Msa0772710:CDS
TAAGTACAAGTATTCTATGG+AGG 0.635194 5_2:+47059199 Msa0772710:CDS
CTTCCTGCAAATGGAAAACG+AGG 0.635933 5_2:+47059978 Msa0772710:three_prime_UTR
TAATTACCTGATGAATGAGA+GGG 0.642402 5_2:-47059066 None:intergenic
GTAAATTCAGGAACTTGAGG+AGG 0.652603 5_2:-47057934 None:intergenic
ACTATTATCTCTTCAACCAG+AGG 0.664964 5_2:-47057637 None:intergenic
GTTATGGCGGATCTAGTGAG+TGG 0.668719 5_2:+47057496 Msa0772710:CDS
TGAAGGACATGGTACTATGG+AGG 0.672764 5_2:+47059166 Msa0772710:CDS
AGATCAAGAGATGCATGGAG+TGG 0.673853 5_2:+47060004 Msa0772710:three_prime_UTR
TAATACAACCAATTCGAGAG+GGG 0.675100 5_2:-47059910 None:intergenic
ATGTAGGCAAAGGTAAAGTG+GGG 0.682400 5_2:+47057351 Msa0772710:five_prime_UTR
CCAATTCAAGCATTCAACAG+AGG 0.722891 5_2:+47057559 Msa0772710:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTAAAACTTTCTTTTTT+TGG - chr5_2:47058639-47058658 None:intergenic 10.0%
!! AAATATAAGATCTTATAATC+TGG - chr5_2:47058425-47058444 None:intergenic 15.0%
!! AAAAAAAAAATTGTTGAACA+AGG - chr5_2:47058503-47058522 None:intergenic 15.0%
!!! TCATTTTTATAAGAAACAAA+TGG + chr5_2:47058677-47058696 Msa0772710:intron 15.0%
!!! CATTTTTATAAGAAACAAAT+GGG + chr5_2:47058678-47058697 Msa0772710:intron 15.0%
!! TTATTTATTGTGTGAAATTT+AGG + chr5_2:47059630-47059649 Msa0772710:intron 15.0%
!! TATTTATTGTGTGAAATTTA+GGG + chr5_2:47059631-47059650 Msa0772710:intron 15.0%
!! TTCCTAACATTTATTTATAT+AGG + chr5_2:47059842-47059861 Msa0772710:CDS 15.0%
!! TACCTATATAAATAAATGTT+AGG - chr5_2:47059847-47059866 None:intergenic 15.0%
!!! TTTTTTTTGTTTTGTTCAAT+AGG + chr5_2:47059936-47059955 Msa0772710:three_prime_UTR 15.0%
!!! ATATCCAATATTATTAGTCT+TGG + chr5_2:47058346-47058365 Msa0772710:intron 20.0%
!!! AAGATCTTATATTTTTTGAC+AGG + chr5_2:47058431-47058450 Msa0772710:intron 20.0%
!! TCATCATATCATATATTGAA+AGG + chr5_2:47058575-47058594 Msa0772710:intron 20.0%
!! CATCATATCATATATTGAAA+GGG + chr5_2:47058576-47058595 Msa0772710:intron 20.0%
!!! TTGTTTCTTATAAAAATGAC+CGG - chr5_2:47058676-47058695 None:intergenic 20.0%
!!! GTACATTTTATTGACTTTTA+AGG + chr5_2:47058793-47058812 Msa0772710:intron 20.0%
!! ATGATATTGTTCAAATTCAA+AGG - chr5_2:47058979-47058998 None:intergenic 20.0%
!! GGTAAAAAGTAGATAATTAA+AGG - chr5_2:47059892-47059911 None:intergenic 20.0%
!! GTTGGATAATAAATTTGATT+TGG + chr5_2:47060030-47060049 Msa0772710:three_prime_UTR 20.0%
!!! TAGATCCATTTTAGTTTAAA+TGG + chr5_2:47060074-47060093 Msa0772710:three_prime_UTR 20.0%
! GAACTCAAGTTATTTATAGA+TGG - chr5_2:47057404-47057423 None:intergenic 25.0%
! CATTATTACTTGCATAGTTA+TGG + chr5_2:47057480-47057499 Msa0772710:CDS 25.0%
! TTGTTCCATTTGAGAATAAA+AGG + chr5_2:47057842-47057861 Msa0772710:CDS 25.0%
! TGTTCCATTTGAGAATAAAA+GGG + chr5_2:47057843-47057862 Msa0772710:CDS 25.0%
!! AAATCCCTTTTATTCTCAAA+TGG - chr5_2:47057850-47057869 None:intergenic 25.0%
! TCTTCAAATGAATCTTTCTT+TGG + chr5_2:47058056-47058075 Msa0772710:CDS 25.0%
! TCATCCAAGACTAATAATAT+TGG - chr5_2:47058353-47058372 None:intergenic 25.0%
! AAAATAAAAAATACTCCCTC+CGG + chr5_2:47058654-47058673 Msa0772710:intron 25.0%
!!! TCAACTAAAGTTGATGTATT+TGG - chr5_2:47058740-47058759 None:intergenic 25.0%
!! CCTTTTTATAAGAGACAAAT+TGG - chr5_2:47058763-47058782 None:intergenic 25.0%
! CCAATTTGTCTCTTATAAAA+AGG + chr5_2:47058760-47058779 Msa0772710:intron 25.0%
! TCAATAAAATGTACTATCTC+CGG - chr5_2:47058787-47058806 None:intergenic 25.0%
! ATATATAGTATGTATTGCCT+CGG - chr5_2:47058836-47058855 None:intergenic 25.0%
! TTAATATGGGTAATGTTAAC+CGG - chr5_2:47058883-47058902 None:intergenic 25.0%
! TATTGTTCAAATTCAAAGGT+AGG - chr5_2:47058975-47058994 None:intergenic 25.0%
!!! TTCATCAGGTAATTATTTTG+TGG + chr5_2:47059074-47059093 Msa0772710:CDS 25.0%
! AACTAAGTACAAGTATTCTA+TGG + chr5_2:47059196-47059215 Msa0772710:CDS 25.0%
! TAGTACTATTGTTCTAATTG+CGG + chr5_2:47059233-47059252 Msa0772710:CDS 25.0%
! AATGTCTCATATATGTGTAT+TGG + chr5_2:47059512-47059531 Msa0772710:intron 25.0%
!!! ACTTATTCATTCTAAAGTGT+TGG + chr5_2:47059572-47059591 Msa0772710:intron 25.0%
!! CATGGATTGAAAAATCAATA+AGG + chr5_2:47059723-47059742 Msa0772710:CDS 25.0%
!!! ATCCATTTTAGTTTAAATGG+TGG + chr5_2:47060077-47060096 Msa0772710:three_prime_UTR 25.0%
!!! TCCATTTTAGTTTAAATGGT+GGG + chr5_2:47060078-47060097 Msa0772710:three_prime_UTR 25.0%
!! CTCTTTTATATAAGCTTCAA+AGG - chr5_2:47060240-47060259 None:intergenic 25.0%
! CTTGAGATGATACTTTAATT+CGG - chr5_2:47060312-47060331 None:intergenic 25.0%
! TAGATGGAGATTGTGATTTT+TGG - chr5_2:47057388-47057407 None:intergenic 30.0%
! GTGGAATCAAACAAATGTTT+TGG - chr5_2:47057445-47057464 None:intergenic 30.0%
!!! AATGCGTTTTGTTGTTTTTG+TGG - chr5_2:47057464-47057483 None:intergenic 30.0%
TATTACTTGCATAGTTATGG+CGG + chr5_2:47057483-47057502 Msa0772710:CDS 30.0%
ACTAATTCTGTATCTTCACT+TGG - chr5_2:47057685-47057704 None:intergenic 30.0%
AGATTCATGGGAATCAAATA+AGG + chr5_2:47057896-47057915 Msa0772710:CDS 30.0%
! ATGGGAATCAAATAAGGTTT+TGG + chr5_2:47057902-47057921 Msa0772710:CDS 30.0%
!! AATCAAATAAGGTTTTGGTG+TGG + chr5_2:47057907-47057926 Msa0772710:CDS 30.0%
!! AAGATTGAGTTGATCAAGAA+TGG + chr5_2:47057985-47058004 Msa0772710:CDS 30.0%
GAAAGATTCATTTGAAGAGT+GGG - chr5_2:47058054-47058073 None:intergenic 30.0%
AGAAAGATTCATTTGAAGAG+TGG - chr5_2:47058055-47058074 None:intergenic 30.0%
TGATTGAATTCCAAATACCT+TGG - chr5_2:47058092-47058111 None:intergenic 30.0%
! TGTACAATGTACTTCAAGTT+GGG - chr5_2:47058230-47058249 None:intergenic 30.0%
TTGTACAATGTACTTCAAGT+TGG - chr5_2:47058231-47058250 None:intergenic 30.0%
GAAGATGCAATTAACGAATT+GGG + chr5_2:47058299-47058318 Msa0772710:intron 30.0%
!! TATTAGTCTTGGATGATGTT+TGG + chr5_2:47058357-47058376 Msa0772710:intron 30.0%
TATAATCTGGCAATTGGAAT+CGG - chr5_2:47058412-47058431 None:intergenic 30.0%
AGATCTTATAATCTGGCAAT+TGG - chr5_2:47058418-47058437 None:intergenic 30.0%
AAAATTGTTGAACAAGGTGA+CGG - chr5_2:47058497-47058516 None:intergenic 30.0%
TTCTTATAAAAATGACCGGA+GGG - chr5_2:47058672-47058691 None:intergenic 30.0%
TTTCTTATAAAAATGACCGG+AGG - chr5_2:47058673-47058692 None:intergenic 30.0%
TTGTCTCTTATAAAAAGGAC+CGG + chr5_2:47058765-47058784 Msa0772710:intron 30.0%
! AATTGATAACACAATTTCCG+AGG + chr5_2:47058816-47058835 Msa0772710:intron 30.0%
TGTAGACATTGGAAATATTG+TGG - chr5_2:47059016-47059035 None:intergenic 30.0%
TAATTACCTGATGAATGAGA+GGG - chr5_2:47059069-47059088 None:intergenic 30.0%
ATAATTACCTGATGAATGAG+AGG - chr5_2:47059070-47059089 None:intergenic 30.0%
! AATGACAGGCAATAATTTTG+TGG + chr5_2:47059119-47059138 Msa0772710:CDS 30.0%
TAAGTACAAGTATTCTATGG+AGG + chr5_2:47059199-47059218 Msa0772710:CDS 30.0%
! TCCAATATGCTTACACTTTT+CGG + chr5_2:47059354-47059373 Msa0772710:CDS 30.0%
GAAATTTGCATTAGAAGTAG+AGG - chr5_2:47059400-47059419 None:intergenic 30.0%
TTCCATTATGTAGACTAACA+CGG + chr5_2:47059427-47059446 Msa0772710:intron 30.0%
CAAAACTAAAGTGTGTCTAA+TGG - chr5_2:47059538-47059557 None:intergenic 30.0%
GGATTGAAAAATCAATAAGG+TGG + chr5_2:47059726-47059745 Msa0772710:CDS 30.0%
TGGTAAGCATAAAGAAAGAT+TGG + chr5_2:47059756-47059775 Msa0772710:CDS 30.0%
!! TTGTTGGTAATCTCTTATGT+AGG + chr5_2:47059806-47059825 Msa0772710:CDS 30.0%
TTAATACAACCAATTCGAGA+GGG - chr5_2:47059914-47059933 None:intergenic 30.0%
ATTAATACAACCAATTCGAG+AGG - chr5_2:47059915-47059934 None:intergenic 30.0%
CTAACACTTCATTAGTTCAA+TGG - chr5_2:47060057-47060076 None:intergenic 30.0%
!!! CCATTTTAGTTTAAATGGTG+GGG + chr5_2:47060079-47060098 Msa0772710:three_prime_UTR 30.0%
AAACCAAGTCAGTAAAAACA+AGG + chr5_2:47060274-47060293 Msa0772710:three_prime_UTR 30.0%
!!! TAACCTTGTTTTTACTGACT+TGG - chr5_2:47060280-47060299 None:intergenic 30.0%
AAATGTAGGCAAAGGTAAAG+TGG + chr5_2:47057349-47057368 Msa0772710:five_prime_UTR 35.0%
AATGTAGGCAAAGGTAAAGT+GGG + chr5_2:47057350-47057369 Msa0772710:five_prime_UTR 35.0%
CTCTGTTGAATGCTTGAATT+GGG - chr5_2:47057561-47057580 None:intergenic 35.0%
CAGAAAAACTTGCAATGCTT+TGG + chr5_2:47057612-47057631 Msa0772710:CDS 35.0%
ACTATTATCTCTTCAACCAG+AGG - chr5_2:47057640-47057659 None:intergenic 35.0%
! AATAGTTTGCAACGATTCGT+TGG + chr5_2:47057654-47057673 Msa0772710:CDS 35.0%
! ATAGTTTGCAACGATTCGTT+GGG + chr5_2:47057655-47057674 Msa0772710:CDS 35.0%
GAATTAGTAGAGACTATCAC+AGG + chr5_2:47057697-47057716 Msa0772710:CDS 35.0%
GACAAACAGTTCTTGAAAGT+AGG - chr5_2:47057757-47057776 None:intergenic 35.0%
TTCTTGCGGAAAAGATTCAT+GGG + chr5_2:47057884-47057903 Msa0772710:CDS 35.0%
AAGTTCCTGAATTTACTCCA+AGG + chr5_2:47057941-47057960 Msa0772710:CDS 35.0%
CAATCTTCAACTCAGTGAAA+AGG - chr5_2:47057972-47057991 None:intergenic 35.0%
! GTTTCTGTGTTTCTTTTGAC+TGG + chr5_2:47058009-47058028 Msa0772710:CDS 35.0%
!! TGTGTTTCTTTTGACTGGTT+TGG + chr5_2:47058014-47058033 Msa0772710:CDS 35.0%
!!! GTGTTTCTTTTGACTGGTTT+GGG + chr5_2:47058015-47058034 Msa0772710:CDS 35.0%
! ATCCACAAATCCAAGGTATT+TGG + chr5_2:47058079-47058098 Msa0772710:intron 35.0%
TTCCAAATACCTTGGATTTG+TGG - chr5_2:47058084-47058103 None:intergenic 35.0%
GAAAATGTTTCCACGAAACT+TGG - chr5_2:47058186-47058205 None:intergenic 35.0%
CGAAGATGCAATTAACGAAT+TGG + chr5_2:47058298-47058317 Msa0772710:intron 35.0%
! ACGAATTGGGACTTCTATTT+TGG + chr5_2:47058312-47058331 Msa0772710:intron 35.0%
AAATTGTTGAACAAGGTGAC+GGG - chr5_2:47058496-47058515 None:intergenic 35.0%
!! TCTGTTGTTTCTGTAGACAT+TGG - chr5_2:47059027-47059046 None:intergenic 35.0%
!! AATTATTTTGTGGCGCAAGA+TGG + chr5_2:47059084-47059103 Msa0772710:CDS 35.0%
! AATTTTGTGGCGCAAAATGA+TGG + chr5_2:47059132-47059151 Msa0772710:CDS 35.0%
GTGTAAGCATATTGGAATAG+AGG - chr5_2:47059350-47059369 None:intergenic 35.0%
TTTGCATTAGAAGTAGAGGA+AGG - chr5_2:47059396-47059415 None:intergenic 35.0%
! ATCCGTGTTAGTCTACATAA+TGG - chr5_2:47059432-47059451 None:intergenic 35.0%
! GGTGCTATGTGCTAATTAAT+AGG + chr5_2:47059593-47059612 Msa0772710:intron 35.0%
AATTCAAGCCTCAACAAGTT+TGG - chr5_2:47059662-47059681 None:intergenic 35.0%
AAAGATTGGCATGTGTTTGT+GGG + chr5_2:47059770-47059789 Msa0772710:CDS 35.0%
TAATACAACCAATTCGAGAG+GGG - chr5_2:47059913-47059932 None:intergenic 35.0%
TGTTCAATAGGTATAGTAGC+TGG + chr5_2:47059948-47059967 Msa0772710:three_prime_UTR 35.0%
CCCCACCATTTAAACTAAAA+TGG - chr5_2:47060082-47060101 None:intergenic 35.0%
GATGATACTTTAATTCGGAG+AGG - chr5_2:47060307-47060326 None:intergenic 35.0%
ATGTAGGCAAAGGTAAAGTG+GGG + chr5_2:47057351-47057370 Msa0772710:five_prime_UTR 40.0%
CCTCTGTTGAATGCTTGAAT+TGG - chr5_2:47057562-47057581 None:intergenic 40.0%
CCAATTCAAGCATTCAACAG+AGG + chr5_2:47057559-47057578 Msa0772710:CDS 40.0%
AAGGGATTTGATGAAGCTGT+TGG + chr5_2:47057861-47057880 Msa0772710:CDS 40.0%
GTTCTTGCGGAAAAGATTCA+TGG + chr5_2:47057883-47057902 Msa0772710:CDS 40.0%
GTAAATTCAGGAACTTGAGG+AGG - chr5_2:47057937-47057956 None:intergenic 40.0%
GGAGTAAATTCAGGAACTTG+AGG - chr5_2:47057940-47057959 None:intergenic 40.0%
TCAAGCCTTGGAGTAAATTC+AGG - chr5_2:47057949-47057968 None:intergenic 40.0%
TCTTTGGATCCACAAATCCA+AGG + chr5_2:47058072-47058091 Msa0772710:CDS 40.0%
!! TCTTCGTCACTTTGAAACTC+AGG - chr5_2:47058284-47058303 None:intergenic 40.0%
GATATGATGACAGAATGCTG+TGG - chr5_2:47058565-47058584 None:intergenic 40.0%
TATGCTCCTAAATCAGTGTC+CGG + chr5_2:47058853-47058872 Msa0772710:intron 40.0%
TGATGGCAATGACATGTTGA+AGG + chr5_2:47059149-47059168 Msa0772710:CDS 40.0%
CAATGACATGTTGAAGGACA+TGG + chr5_2:47059155-47059174 Msa0772710:CDS 40.0%
TGTTGAAGGACATGGTACTA+TGG + chr5_2:47059163-47059182 Msa0772710:CDS 40.0%
GTACAAGTATTCTATGGAGG+AGG + chr5_2:47059202-47059221 Msa0772710:CDS 40.0%
AACAGCAACATGGTTGTTGA+TGG + chr5_2:47059257-47059276 Msa0772710:CDS 40.0%
ACTTCCTCACTCTCATTTCA+TGG + chr5_2:47059295-47059314 Msa0772710:CDS 40.0%
! GCCGAAAAGTGTAAGCATAT+TGG - chr5_2:47059358-47059377 None:intergenic 40.0%
TTAGAAGTAGAGGAAGGAGA+AGG - chr5_2:47059390-47059409 None:intergenic 40.0%
AGAAGAAACAGCTGTGAGAA+TGG + chr5_2:47059685-47059704 Msa0772710:CDS 40.0%
ATCAATAAGGTGGTTGTTCG+TGG + chr5_2:47059736-47059755 Msa0772710:CDS 40.0%
GAAAGATTGGCATGTGTTTG+TGG + chr5_2:47059769-47059788 Msa0772710:CDS 40.0%
AAATGTTAGGAAGTCCATGC+AGG - chr5_2:47059834-47059853 None:intergenic 40.0%
! ACTTTTTACCCCTCTCGAAT+TGG + chr5_2:47059902-47059921 Msa0772710:three_prime_UTR 40.0%
! TCGTTTTCCATTTGCAGGAA+GGG - chr5_2:47059979-47059998 None:intergenic 40.0%
! TATCCTCGTTTTCCATTTGC+AGG - chr5_2:47059984-47060003 None:intergenic 40.0%
GGATAAGATCAAGAGATGCA+TGG + chr5_2:47059999-47060018 Msa0772710:three_prime_UTR 40.0%
! TTTTCTGCCTCTTTCGAGAG+TGG - chr5_2:47057599-47057618 None:intergenic 45.0%
! TGAAGCTTTGCTTGTTGAGC+TGG - chr5_2:47057784-47057803 None:intergenic 45.0%
AGAAACTTGACGCTCTTCAC+TGG + chr5_2:47057806-47057825 Msa0772710:CDS 45.0%
!! GATGAAGCTGTTGGTTCTTG+CGG + chr5_2:47057870-47057889 Msa0772710:CDS 45.0%
TCAGTGAAAAGGTCAAGCCT+TGG - chr5_2:47057961-47057980 None:intergenic 45.0%
! TCTTGACTAGCCAAGTTTCG+TGG + chr5_2:47058173-47058192 Msa0772710:intron 45.0%
AAGTTGGGAGTTCTTGAGAC+GGG - chr5_2:47058215-47058234 None:intergenic 45.0%
! CAAGTTGGGAGTTCTTGAGA+CGG - chr5_2:47058216-47058235 None:intergenic 45.0%
TGATGACAGAATGCTGTGGT+TGG - chr5_2:47058561-47058580 None:intergenic 45.0%
GGTCATGTTAACTAGTACCC+CGG + chr5_2:47058597-47058616 Msa0772710:intron 45.0%
GTCATGTTAACTAGTACCCC+GGG + chr5_2:47058598-47058617 Msa0772710:intron 45.0%
TCATGTTAACTAGTACCCCG+GGG + chr5_2:47058599-47058618 Msa0772710:intron 45.0%
ATGCTCCTAAATCAGTGTCC+GGG + chr5_2:47058854-47058873 Msa0772710:intron 45.0%
GAATGAGAGGGAGTTGAGAA+AGG - chr5_2:47059057-47059076 None:intergenic 45.0%
TCAACTCCCTCTCATTCATC+AGG + chr5_2:47059060-47059079 Msa0772710:CDS 45.0%
! TATTTTGTGGCGCAAGATGG+TGG + chr5_2:47059087-47059106 Msa0772710:CDS 45.0%
GGTGGCAAGAACATAATGAC+AGG + chr5_2:47059105-47059124 Msa0772710:CDS 45.0%
TGAAGGACATGGTACTATGG+AGG + chr5_2:47059166-47059185 Msa0772710:CDS 45.0%
TAATTGCGGCAACAGCAACA+TGG + chr5_2:47059247-47059266 Msa0772710:CDS 45.0%
TGAGCCATGAAATGAGAGTG+AGG - chr5_2:47059302-47059321 None:intergenic 45.0%
GTAGACTAACACGGATACGT+AGG + chr5_2:47059436-47059455 Msa0772710:intron 45.0%
TCAAGCCTCAACAAGTTTGG+TGG - chr5_2:47059659-47059678 None:intergenic 45.0%
GCATGTGTTTGTGGGAGTTA+TGG + chr5_2:47059778-47059797 Msa0772710:CDS 45.0%
! CTCGTTTTCCATTTGCAGGA+AGG - chr5_2:47059980-47059999 None:intergenic 45.0%
CTTCCTGCAAATGGAAAACG+AGG + chr5_2:47059978-47059997 Msa0772710:three_prime_UTR 45.0%
AGATCAAGAGATGCATGGAG+TGG + chr5_2:47060004-47060023 Msa0772710:three_prime_UTR 45.0%
! AGATGCATGGAGTGGTTTGT+TGG + chr5_2:47060012-47060031 Msa0772710:three_prime_UTR 45.0%
!!! TTTAAACTTTATTTTAATAT+GGG - chr5_2:47058896-47058915 None:intergenic 5.0%
!!! ATTTAAACTTTATTTTAATA+TGG - chr5_2:47058897-47058916 None:intergenic 5.0%
!! GTTATGGCGGATCTAGTGAG+TGG + chr5_2:47057496-47057515 Msa0772710:CDS 50.0%
TACCCTTCCACTCTCGAAAG+AGG + chr5_2:47057589-47057608 Msa0772710:CDS 50.0%
TGCCTCTTTCGAGAGTGGAA+GGG - chr5_2:47057594-47057613 None:intergenic 50.0%
! TTGCAATGCTTTGGCTCCTC+TGG + chr5_2:47057621-47057640 Msa0772710:CDS 50.0%
GTCTTGGATGATGTTTGGCC+TGG + chr5_2:47058362-47058381 Msa0772710:intron 50.0%
TCAACAAGTCCTTCTGAGCC+AGG - chr5_2:47058383-47058402 None:intergenic 50.0%
!! TGTTGAACAAGGTGACGGGT+AGG - chr5_2:47058492-47058511 None:intergenic 50.0%
TAAATCAGTGTCCGGGACAC+CGG + chr5_2:47058861-47058880 Msa0772710:intron 50.0%
GGTAATGTTAACCGGTGTCC+CGG - chr5_2:47058875-47058894 None:intergenic 50.0%
AGGACATGGTACTATGGAGG+AGG + chr5_2:47059169-47059188 Msa0772710:CDS 50.0%
!! GGGAACCACCAAACTTGTTG+AGG + chr5_2:47059651-47059670 Msa0772710:intron 50.0%
TGGCAAAGACAGTTCGAGCA+TGG + chr5_2:47059705-47059724 Msa0772710:CDS 50.0%
GGGAGTTATGGCTACGTTGT+TGG + chr5_2:47059790-47059809 Msa0772710:CDS 50.0%
CTCTTATGTAGGAGCCTGCA+TGG + chr5_2:47059817-47059836 Msa0772710:CDS 50.0%
GGTATGACCCTTCCTGCAAA+TGG + chr5_2:47059969-47059988 Msa0772710:three_prime_UTR 50.0%
CTAGTGAGTGGAGCTGTCGT+TGG + chr5_2:47057508-47057527 Msa0772710:CDS 55.0%
CTGCCTCTTTCGAGAGTGGA+AGG - chr5_2:47057595-47057614 None:intergenic 55.0%
CAAACACTGTTTGCCCGCTG+TGG + chr5_2:47058248-47058267 Msa0772710:intron 55.0%
TGTTTGCCCGCTGTGGACTT+TGG + chr5_2:47058255-47058274 Msa0772710:intron 55.0%
AGGCATCCAAAGTCCACAGC+GGG - chr5_2:47058264-47058283 None:intergenic 55.0%
CAGGCATCCAAAGTCCACAG+CGG - chr5_2:47058265-47058284 None:intergenic 55.0%
GATGTTTGGCCTGGCTCAGA+AGG + chr5_2:47058371-47058390 Msa0772710:intron 55.0%
TTAACTAGTACCCCGGGGCA+CGG + chr5_2:47058604-47058623 Msa0772710:intron 55.0%
!! GGTGTCCCGGACACTGATTT+AGG - chr5_2:47058862-47058881 None:intergenic 55.0%
TGGAGCTGTCGTTGGAGCTG+TGG + chr5_2:47057516-47057535 Msa0772710:CDS 60.0%
AGCTGTCGTTGGAGCTGTGG+TGG + chr5_2:47057519-47057538 Msa0772710:CDS 60.0%
GCTGTCGTTGGAGCTGTGGT+GGG + chr5_2:47057520-47057539 Msa0772710:CDS 60.0%
TAACTAGTACCCCGGGGCAC+GGG + chr5_2:47058605-47058624 Msa0772710:intron 60.0%
TGGTTCCTTAACCCGTGCCC+CGG - chr5_2:47058619-47058638 None:intergenic 60.0%
! CGTTGGAGCTGTGGTGGGAG+AGG + chr5_2:47057525-47057544 Msa0772710:CDS 65.0%
GTTCCTTAACCCGTGCCCCG+GGG - chr5_2:47058617-47058636 None:intergenic 65.0%
GGTTCCTTAACCCGTGCCCC+GGG - chr5_2:47058618-47058637 None:intergenic 65.0%
! GTACCCCGGGGCACGGGTTA+AGG + chr5_2:47058611-47058630 Msa0772710:intron 70.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 47057347 47060340 47057347 ID=Msa0772710;Name=Msa0772710
chr5_2 mRNA 47057347 47060340 47057347 ID=Msa0772710-mRNA-1;Parent=Msa0772710;Name=Msa0772710-mRNA-1;_AED=0.09;_eAED=0.09;_QI=77|0.5|1|1|1|1|3|469|410
chr5_2 exon 47057347 47058093 47057347 ID=Msa0772710-mRNA-1:exon:14096;Parent=Msa0772710-mRNA-1
chr5_2 exon 47059037 47059379 47059037 ID=Msa0772710-mRNA-1:exon:14097;Parent=Msa0772710-mRNA-1
chr5_2 exon 47059652 47060340 47059652 ID=Msa0772710-mRNA-1:exon:14098;Parent=Msa0772710-mRNA-1
chr5_2 five_prime_UTR 47057347 47057423 47057347 ID=Msa0772710-mRNA-1:five_prime_utr;Parent=Msa0772710-mRNA-1
chr5_2 CDS 47057424 47058093 47057424 ID=Msa0772710-mRNA-1:cds;Parent=Msa0772710-mRNA-1
chr5_2 CDS 47059037 47059379 47059037 ID=Msa0772710-mRNA-1:cds;Parent=Msa0772710-mRNA-1
chr5_2 CDS 47059652 47059871 47059652 ID=Msa0772710-mRNA-1:cds;Parent=Msa0772710-mRNA-1
chr5_2 three_prime_UTR 47059872 47060340 47059872 ID=Msa0772710-mRNA-1:three_prime_utr;Parent=Msa0772710-mRNA-1
Gene Sequence

>Msa0772710

ATGACTTTTCTTTCTCATCCAAAACATTTGTTTGATTCCACAAAAACAACAAAACGCATTATTACTTGCATAGTTATGGCGGATCTAGTGAGTGGAGCTGTCGTTGGAGCTGTGGTGGGAGAGGCTATGAAATACCCAATTCAAGCATTCAACAGAGGTCGACATTACCCTTCCACTCTCGAAAGAGGCAGAAAAACTTGCAATGCTTTGGCTCCTCTGGTTGAAGAGATAATAGTTTGCAACGATTCGTTGGGTCGACCAAGTGAAGATACAGAATTAGTAGAGACTATCACAGGAGAGCTCACAGAGCTTGTACGAAAAAGCAAGAAGCCTACTTTCAAGAACTGTTTGTCTTGTCCAGCTCAACAAGCAAAGCTTCAGAAGAAACTTGACGCTCTTCACTGGCATTCGTCTGTTGTTGTTCCATTTGAGAATAAAAGGGATTTGATGAAGCTGTTGGTTCTTGCGGAAAAGATTCATGGGAATCAAATAAGGTTTTGGTGTGGTGCTCCTCCTCAAGTTCCTGAATTTACTCCAAGGCTTGACCTTTTCACTGAGTTGAAGATTGAGTTGATCAAGAATGGTGTTTCTGTGTTTCTTTTGACTGGTTTGGGTGCTTCTGTTAAACCCACTCTTCAAATGAATCTTTCTTTGGATCCACAAATCCAAGAAACAACAGAAACAAAACCTTTCTCAACTCCCTCTCATTCATCAGGTAATTATTTTGTGGCGCAAGATGGTGGCAAGAACATAATGACAGGCAATAATTTTGTGGCGCAAAATGATGGCAATGACATGTTGAAGGACATGGTACTATGGAGGAGGAAGAAACTAAGTACAAGTATTCTATGGAGGAGGAAGAAACTTAGTACTATTGTTCTAATTGCGGCAACAGCAACATGGTTGTTGATGGAAGTTTATCAATTTAACTTCCTCACTCTCATTTCATGGCTCACTATATTTGTTGTTACTTCAATATTCCTCTATTCCAATATGCTTACACTTTTCGGCAAGGAACCACCAAACTTGTTGAGGCTTGAATTGAAAGAAGAAACAGCTGTGAGAATGGCAAAGACAGTTCGAGCATGGATTGAAAAATCAATAAGGTGGTTGTTCGTGGTAAGCATAAAGAAAGATTGGCATGTGTTTGTGGGAGTTATGGCTACGTTGTTGGTAATCTCTTATGTAGGAGCCTGCATGGACTTCCTAACATTTATTTATATAGGTACATGA
Protein sequence

>Msa0772710

MTFLSHPKHLFDSTKTTKRIITCIVMADLVSGAVVGAVVGEAMKYPIQAFNRGRHYPSTLERGRKTCNALAPLVEEIIVCNDSLGRPSEDTELVETITGELTELVRKSKKPTFKNCLSCPAQQAKLQKKLDALHWHSSVVVPFENKRDLMKLLVLAEKIHGNQIRFWCGAPPQVPEFTPRLDLFTELKIELIKNGVSVFLLTGLGASVKPTLQMNLSLDPQIQETTETKPFSTPSHSSGNYFVAQDGGKNIMTGNNFVAQNDGNDMLKDMVLWRRKKLSTSILWRRKKLSTIVLIAATATWLLMEVYQFNFLTLISWLTIFVVTSIFLYSNMLTLFGKEPPNLLRLELKEETAVRMAKTVRAWIEKSIRWLFVVSIKKDWHVFVGVMATLLVISYVGACMDFLTFIYIGT*