AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0259580

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0259580 MtrunA17_Chr2g0333851 95.327 321 6 5 1 317 1 316 0.0 614
Msa0259580 MtrunA17_Chr4g0001361 59.202 326 108 8 5 317 4 317 1.54e-105 311
Msa0259580 MtrunA17_Chr1g0150281 46.996 283 126 7 41 314 9 276 1.00e-71 223
Msa0259580 MtrunA17_Chr3g0136961 45.818 275 116 7 56 314 29 286 9.91e-69 216
Msa0259580 MtrunA17_Chr1g0150271 36.940 268 133 7 51 316 6 239 6.66e-40 140
Msa0259580 MtrunA17_Chr7g0270831 27.900 319 145 8 57 310 100 398 3.71e-30 118
Msa0259580 MtrunA17_Chr4g0075201 30.804 224 112 5 49 236 116 332 6.06e-28 113
Msa0259580 MtrunA17_Chr2g0288871 33.333 237 94 8 49 231 139 365 1.96e-26 109
Msa0259580 MtrunA17_Chr3g0099641 30.435 230 111 6 47 231 86 311 4.90e-24 101
Msa0259580 MtrunA17_Chr4g0051181 54.321 81 32 2 151 231 228 303 6.95e-24 101
Msa0259580 MtrunA17_Chr2g0302391 31.203 266 124 8 55 275 70 321 1.64e-23 100
Msa0259580 MtrunA17_Chr7g0216891 48.810 84 41 1 146 229 196 277 7.85e-23 99.0
Msa0259580 MtrunA17_Chr4g0060271 52.500 80 36 1 152 231 238 315 8.22e-23 99.0
Msa0259580 MtrunA17_Chr7g0270191 51.852 81 36 2 151 231 127 204 1.62e-22 95.1
Msa0259580 MtrunA17_Chr1g0194481 55.844 77 31 2 156 232 145 218 1.77e-22 95.9
Msa0259580 MtrunA17_Chr2g0294231 54.321 81 34 2 156 236 190 267 5.49e-22 95.1
Msa0259580 MtrunA17_Chr4g0051881 51.948 77 34 2 156 232 165 238 1.96e-21 93.2
Msa0259580 MtrunA17_Chr4g0025051 48.718 78 38 1 155 232 321 396 4.61e-21 93.6
Msa0259580 MtrunA17_Chr2g0322391 31.452 248 88 9 56 231 172 409 9.50e-21 92.8
Msa0259580 MtrunA17_Chr1g0188941 41.667 96 40 2 137 232 32 111 1.79e-20 85.1
Msa0259580 MtrunA17_Chr5g0445311 57.812 64 26 1 169 232 362 424 5.72e-20 90.5
Msa0259580 MtrunA17_Chr5g0445311 47.761 67 30 3 172 238 201 262 6.92e-12 66.2
Msa0259580 MtrunA17_Chr5g0443131 51.948 77 34 2 156 232 144 217 7.07e-20 88.2
Msa0259580 MtrunA17_Chr4g0025061 34.286 140 59 3 155 263 346 483 7.22e-20 90.1
Msa0259580 MtrunA17_Chr4g0004911 40.909 110 49 2 122 231 302 395 7.50e-20 89.7
Msa0259580 MtrunA17_Chr4g0004911 50.769 65 31 1 171 235 184 247 2.10e-16 79.7
Msa0259580 MtrunA17_Chr7g0263531 52.857 70 32 1 162 231 352 420 8.46e-20 89.4
Msa0259580 MtrunA17_Chr7g0263531 55.000 60 25 2 172 231 190 247 5.59e-15 75.1
Msa0259580 MtrunA17_Chr5g0418191 54.688 64 28 1 169 232 318 380 1.15e-19 89.0
Msa0259580 MtrunA17_Chr5g0418191 46.753 77 31 4 172 246 152 220 3.61e-13 69.7
Msa0259580 MtrunA17_Chr3g0101131 57.812 64 26 1 169 232 413 475 1.17e-19 89.4
Msa0259580 MtrunA17_Chr3g0101131 50.000 60 28 2 172 231 244 301 8.47e-12 65.9
Msa0259580 MtrunA17_Chr4g0042831 42.056 107 46 3 137 243 259 349 1.20e-19 88.6
Msa0259580 MtrunA17_Chr4g0042831 47.059 68 35 1 172 239 146 212 4.57e-13 69.3
Msa0259580 MtrunA17_Chr1g0150801 50.667 75 34 2 156 230 89 160 3.13e-19 83.2
Msa0259580 MtrunA17_Chr5g0418781 52.055 73 32 2 156 228 111 180 5.79e-19 85.1
Msa0259580 MtrunA17_Chr3g0079691 41.667 96 40 2 137 232 476 555 5.88e-19 87.4
Msa0259580 MtrunA17_Chr3g0079691 50.820 61 28 2 171 231 280 338 1.35e-12 68.6
Msa0259580 MtrunA17_Chr5g0401821 40.385 104 55 2 125 228 160 256 6.95e-19 84.7
Msa0259580 MtrunA17_Chr5g0432161 40.741 108 48 3 125 232 390 481 7.94e-19 87.0
Msa0259580 MtrunA17_Chr5g0432161 49.206 63 30 2 172 234 245 305 5.43e-14 72.4
Msa0259580 MtrunA17_Chr7g0218081 56.667 60 25 1 169 228 408 466 8.23e-19 86.7
Msa0259580 MtrunA17_Chr7g0218081 44.578 83 42 3 172 252 229 309 2.99e-12 67.0
Msa0259580 MtrunA17_Chr8g0383351 49.315 73 35 2 156 228 146 216 8.34e-19 84.0
Msa0259580 MtrunA17_Chr7g0243891 35.075 134 64 4 126 252 344 461 1.10e-18 86.7
Msa0259580 MtrunA17_Chr7g0243891 47.143 70 35 2 171 240 216 283 1.52e-14 73.9
Msa0259580 MtrunA17_Chr7g0248721 52.564 78 33 3 152 229 124 197 1.18e-18 83.2
Msa0259580 MtrunA17_Chr7g0249311 44.545 110 54 4 126 232 347 452 1.51e-18 86.3
Msa0259580 MtrunA17_Chr7g0249311 50.769 65 28 3 171 233 179 241 5.85e-12 66.2
Msa0259580 MtrunA17_Chr7g0224801 37.302 126 65 3 115 229 28 150 2.01e-18 81.3
Msa0259580 MtrunA17_Chr4g0068541 50.649 77 35 2 155 231 194 267 2.71e-18 84.7
Msa0259580 MtrunA17_Chr1g0147881 44.048 84 43 1 149 228 236 319 3.19e-18 84.0
Msa0259580 MtrunA17_Chr7g0249301 43.750 96 51 3 139 232 258 352 3.70e-18 84.3
Msa0259580 MtrunA17_Chr7g0249301 48.387 62 30 2 170 231 61 120 2.60e-11 63.9
Msa0259580 MtrunA17_Chr3g0129321 30.570 193 110 6 88 273 30 205 4.47e-18 81.3
Msa0259580 MtrunA17_Chr3g0106491 39.091 110 51 3 123 232 332 425 6.55e-18 84.0
Msa0259580 MtrunA17_Chr3g0106491 51.667 60 27 2 172 231 199 256 2.84e-14 73.2
Msa0259580 MtrunA17_Chr4g0053281 56.140 57 25 0 172 228 267 323 8.64e-18 82.8
Msa0259580 MtrunA17_Chr8g0386241 43.590 78 43 1 169 246 101 177 1.04e-17 79.7
Msa0259580 MtrunA17_Chr5g0447701 50.000 78 36 2 155 232 238 312 1.19e-17 82.8
Msa0259580 MtrunA17_Chr3g0129361 47.945 73 35 2 156 228 131 200 1.61e-17 80.1
Msa0259580 MtrunA17_Chr8g0335811 39.130 115 60 4 115 228 105 210 5.92e-17 78.6
Msa0259580 MtrunA17_Chr8g0383251 47.368 76 37 2 156 231 95 167 6.85e-17 77.4
Msa0259580 MtrunA17_Chr2g0293481 43.038 79 45 0 168 246 264 342 7.89e-17 80.9
Msa0259580 MtrunA17_Chr1g0184691 51.667 60 29 0 169 228 291 350 1.02e-16 79.7
Msa0259580 MtrunA17_Chr7g0270051 35.238 105 65 1 135 239 20 121 1.12e-16 78.6
Msa0259580 MtrunA17_Chr4g0071521 45.570 79 42 1 151 228 224 302 4.32e-16 77.4
Msa0259580 MtrunA17_Chr7g0238921 45.333 75 30 2 172 236 125 198 5.60e-16 77.4
Msa0259580 MtrunA17_Chr8g0379461 34.821 112 72 1 140 250 45 156 6.12e-16 75.9
Msa0259580 MtrunA17_Chr7g0228941 45.455 66 36 0 163 228 44 109 7.75e-16 72.8
Msa0259580 MtrunA17_Chr8g0348311 37.895 95 53 2 172 266 156 244 1.14e-15 76.6
Msa0259580 MtrunA17_Chr8g0387501 45.161 62 34 0 172 233 130 191 1.68e-15 75.1
Msa0259580 MtrunA17_Chr3g0139021 32.727 110 57 2 124 228 274 371 3.62e-15 75.5
Msa0259580 MtrunA17_Chr4g0024391 37.179 78 47 1 155 232 215 290 7.31e-15 75.1
Msa0259580 MtrunA17_Chr4g0074001 43.836 73 40 1 159 231 347 418 7.31e-15 75.1
Msa0259580 MtrunA17_Chr7g0248691 43.077 65 37 0 169 233 152 216 7.69e-15 73.9
Msa0259580 MtrunA17_Chr2g0284321 43.333 60 34 0 172 231 36 95 5.35e-14 70.1
Msa0259580 MtrunA17_Chr1g0189391 32.800 125 70 5 111 228 37 154 6.32e-14 71.6
Msa0259580 MtrunA17_Chr2g0289181 45.161 62 34 0 171 232 14 75 8.11e-14 69.7
Msa0259580 MtrunA17_Chr2g0319961 36.364 110 66 2 125 231 32 140 1.23e-13 69.7
Msa0259580 MtrunA17_Chr6g0485431 38.202 89 50 4 149 235 133 218 1.35e-13 70.9
Msa0259580 MtrunA17_Chr5g0432071 41.791 67 37 2 172 238 105 169 4.04e-13 67.4
Msa0259580 MtrunA17_Chr1g0202461 43.548 62 35 0 172 233 262 323 7.26e-13 68.6
Msa0259580 MtrunA17_Chr5g0447711 38.667 75 45 1 158 232 217 290 2.19e-12 66.6
Msa0259580 MtrunA17_Chr8g0387981 48.529 68 31 2 163 226 122 189 4.81e-12 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0259580 AT1G80840.1 53.474 331 107 12 1 317 1 298 2.90e-99 295
Msa0259580 AT4G31800.1 45.238 336 135 10 1 316 1 307 1.36e-79 244
Msa0259580 AT4G31800.2 47.328 262 99 7 74 316 1 242 1.79e-68 214
Msa0259580 AT2G25000.2 45.283 265 95 7 57 314 2 223 1.29e-63 201
Msa0259580 AT2G25000.4 45.283 265 95 7 57 314 2 223 1.29e-63 201
Msa0259580 AT2G25000.1 44.364 275 103 7 49 316 37 268 1.50e-63 202
Msa0259580 AT2G25000.3 43.599 289 110 8 33 314 3 245 4.15e-63 200
Msa0259580 AT4G31800.3 46.053 228 88 8 1 208 1 213 1.45e-49 165
Msa0259580 AT5G41570.1 47.872 94 45 3 136 229 66 155 3.99e-23 94.4
Msa0259580 AT4G01720.1 25.143 350 152 8 60 310 105 443 4.14e-23 99.8
Msa0259580 AT4G04450.2 40.187 107 55 1 125 231 161 258 5.19e-23 99.0
Msa0259580 AT1G68150.1 53.086 81 33 2 151 231 220 295 6.97e-23 97.8
Msa0259580 AT4G18170.1 46.535 101 50 3 133 232 135 232 9.07e-23 96.7
Msa0259580 AT4G22070.4 51.190 84 36 2 148 231 279 357 1.02e-22 99.0
Msa0259580 AT4G22070.3 51.190 84 36 2 148 231 279 357 1.02e-22 99.0
Msa0259580 AT4G22070.2 51.190 84 36 2 148 231 279 357 1.02e-22 99.0
Msa0259580 AT4G22070.1 51.190 84 36 2 148 231 279 357 1.02e-22 99.0
Msa0259580 AT1G64000.1 51.316 76 34 2 154 229 99 171 1.19e-22 93.6
Msa0259580 AT1G29860.1 53.659 82 35 2 156 237 123 201 1.55e-22 95.5
Msa0259580 AT4G04450.1 40.187 107 55 1 125 231 255 352 1.69e-22 98.2
Msa0259580 AT1G62300.1 51.190 84 36 2 148 231 294 372 2.47e-22 97.8
Msa0259580 AT5G15130.2 39.655 116 54 3 121 231 141 245 6.70e-22 96.3
Msa0259580 AT5G15130.1 39.655 116 54 3 121 231 183 287 7.08e-22 96.3
Msa0259580 AT4G26640.5 31.527 203 121 5 113 310 144 333 1.09e-21 94.7
Msa0259580 AT4G26640.5 45.070 71 37 2 161 231 25 93 2.01e-12 67.8
Msa0259580 AT4G26640.6 31.527 203 121 5 113 310 144 333 1.09e-21 94.7
Msa0259580 AT4G26640.6 45.070 71 37 2 161 231 25 93 2.01e-12 67.8
Msa0259580 AT4G26640.4 31.527 203 121 5 113 310 144 333 1.09e-21 94.7
Msa0259580 AT4G26640.4 45.070 71 37 2 161 231 25 93 2.01e-12 67.8
Msa0259580 AT4G26640.3 31.527 203 121 5 113 310 144 333 1.09e-21 94.7
Msa0259580 AT4G26640.3 45.070 71 37 2 161 231 25 93 2.01e-12 67.8
Msa0259580 AT1G18860.1 59.677 62 25 0 170 231 238 299 1.16e-21 95.5
Msa0259580 AT4G26640.1 31.527 203 121 5 113 310 248 437 2.53e-21 94.4
Msa0259580 AT4G26640.1 45.070 71 37 2 161 231 129 197 2.19e-12 67.8
Msa0259580 AT4G26640.7 31.527 203 121 5 113 310 280 469 3.44e-21 94.4
Msa0259580 AT4G26640.7 45.070 71 37 2 161 231 161 229 2.31e-12 67.8
Msa0259580 AT5G49520.1 53.247 77 33 2 155 231 207 280 3.60e-21 93.6
Msa0259580 AT5G07100.2 33.987 153 80 4 85 232 65 201 3.60e-21 90.1
Msa0259580 AT5G07100.2 45.455 77 41 1 159 235 12 87 2.82e-16 76.6
Msa0259580 AT5G07100.4 33.987 153 80 4 85 232 65 201 3.60e-21 90.1
Msa0259580 AT5G07100.4 45.455 77 41 1 159 235 12 87 2.82e-16 76.6
Msa0259580 AT5G07100.5 33.987 153 80 4 85 232 65 201 3.60e-21 90.1
Msa0259580 AT5G07100.5 45.455 77 41 1 159 235 12 87 2.82e-16 76.6
Msa0259580 AT5G07100.3 33.987 153 80 4 85 232 65 201 3.60e-21 90.1
Msa0259580 AT5G07100.3 45.455 77 41 1 159 235 12 87 2.82e-16 76.6
Msa0259580 AT4G26640.2 31.527 203 121 5 113 310 320 509 3.94e-21 94.4
Msa0259580 AT4G26640.2 45.070 71 37 2 161 231 201 269 2.34e-12 67.8
Msa0259580 AT2G47260.1 50.000 86 40 2 151 236 156 238 6.14e-21 92.0
Msa0259580 AT5G07100.1 33.987 153 80 4 85 232 158 294 9.00e-21 91.3
Msa0259580 AT5G07100.1 45.455 77 41 1 159 235 105 180 9.74e-16 77.0
Msa0259580 AT3G62340.1 51.163 86 39 2 151 236 100 182 1.13e-20 90.1
Msa0259580 AT5G26170.1 53.425 73 31 2 156 228 100 169 1.28e-20 87.8
Msa0259580 AT2G21900.1 45.000 100 47 3 134 232 77 169 1.44e-20 88.2
Msa0259580 AT1G13960.1 34.694 147 68 3 101 247 366 484 2.70e-20 91.7
Msa0259580 AT1G13960.1 49.254 67 32 2 172 238 230 294 3.06e-13 70.5
Msa0259580 AT1G69310.4 51.282 78 35 2 151 228 129 203 2.95e-20 89.4
Msa0259580 AT1G69310.2 51.282 78 35 2 151 228 129 203 2.95e-20 89.4
Msa0259580 AT1G69310.1 51.282 78 35 2 151 228 129 203 2.95e-20 89.4
Msa0259580 AT1G13960.2 34.694 147 68 3 101 247 213 331 4.81e-20 89.7
Msa0259580 AT1G13960.2 49.254 67 32 2 172 238 77 141 3.44e-13 69.7
Msa0259580 AT5G46350.1 39.683 126 55 6 156 280 170 275 6.16e-20 89.0
Msa0259580 AT5G56270.1 44.000 100 40 3 133 232 464 547 9.08e-20 90.5
Msa0259580 AT5G56270.1 46.053 76 35 3 170 245 272 341 9.08e-13 69.3
Msa0259580 AT2G03340.1 56.250 64 27 1 169 232 413 475 1.21e-19 89.7
Msa0259580 AT2G03340.1 45.833 72 37 2 172 243 251 320 5.86e-14 72.8
Msa0259580 AT1G55600.1 50.000 80 37 2 152 231 290 366 1.83e-19 89.0
Msa0259580 AT5G13080.1 48.750 80 38 2 156 235 54 130 2.42e-19 83.2
Msa0259580 AT3G01080.2 55.556 63 27 1 169 231 342 403 1.57e-18 86.3
Msa0259580 AT3G01080.2 41.333 75 42 2 172 246 206 278 6.74e-11 63.2
Msa0259580 AT1G69810.1 29.365 252 113 8 43 231 15 264 1.67e-18 85.5
Msa0259580 AT3G01080.1 55.556 63 27 1 169 231 304 365 1.77e-18 85.9
Msa0259580 AT3G01080.1 50.000 60 28 2 172 231 168 225 8.33e-11 62.8
Msa0259580 AT5G52830.1 43.182 88 50 0 172 259 166 253 2.34e-18 84.7
Msa0259580 AT5G64810.1 37.931 116 67 3 113 228 56 166 4.55e-18 81.3
Msa0259580 AT2G30250.1 48.276 87 42 2 146 232 305 388 4.56e-18 84.3
Msa0259580 AT2G30250.1 36.538 104 51 4 172 262 167 268 7.04e-13 68.9
Msa0259580 AT3G01970.1 50.000 74 34 2 155 228 51 121 8.52e-18 79.3
Msa0259580 AT2G46130.1 49.367 79 37 2 151 229 12 87 9.35e-18 77.8
Msa0259580 AT2G23320.1 39.683 126 62 4 103 228 186 297 9.91e-18 82.8
Msa0259580 AT1G69810.2 48.750 80 38 2 153 231 129 206 1.18e-17 82.4
Msa0259580 AT4G39410.1 45.783 83 42 2 146 228 200 279 1.20e-17 82.4
Msa0259580 AT2G04880.1 50.704 71 34 1 169 239 305 374 1.29e-17 83.6
Msa0259580 AT2G04880.1 39.744 78 44 3 169 245 109 184 2.05e-12 68.2
Msa0259580 AT2G04880.2 50.704 71 34 1 169 239 281 350 1.39e-17 83.6
Msa0259580 AT2G04880.2 39.744 78 44 3 169 245 109 184 2.04e-12 67.8
Msa0259580 AT2G30590.1 51.515 66 31 1 169 233 311 376 3.72e-17 81.6
Msa0259580 AT2G38470.1 53.125 64 29 1 169 232 360 422 3.73e-17 82.4
Msa0259580 AT2G38470.1 52.459 61 27 2 171 231 184 242 1.96e-14 74.3
Msa0259580 AT2G44745.1 38.655 119 68 4 120 236 95 210 4.23e-17 79.0
Msa0259580 AT4G31550.2 51.667 60 29 0 169 228 243 302 4.28e-17 80.9
Msa0259580 AT4G31550.1 51.667 60 29 0 169 228 244 303 4.45e-17 80.9
Msa0259580 AT4G26440.1 54.688 64 28 1 169 232 370 432 5.04e-17 82.0
Msa0259580 AT4G26440.1 35.185 108 59 4 172 279 179 275 1.03e-11 65.9
Msa0259580 AT1G29280.1 50.000 60 30 0 172 231 75 134 5.62e-17 79.7
Msa0259580 AT2G34830.1 35.043 117 70 1 123 233 161 277 6.74e-17 81.3
Msa0259580 AT2G34830.2 35.043 117 70 1 123 233 170 286 8.10e-17 80.9
Msa0259580 AT5G43290.1 42.857 84 46 2 145 228 89 170 8.89e-17 79.3
Msa0259580 AT3G04670.1 51.667 60 29 0 169 228 260 319 2.57e-16 79.0
Msa0259580 AT4G01250.1 35.714 98 63 0 172 269 129 226 2.61e-16 78.2
Msa0259580 AT1G69310.3 50.000 78 32 3 151 228 129 199 2.71e-16 78.2
Msa0259580 AT4G24240.1 44.304 79 43 1 151 228 260 338 3.38e-16 78.6
Msa0259580 AT2G37260.3 37.500 120 64 3 113 228 213 325 6.71e-16 77.8
Msa0259580 AT2G37260.3 46.667 75 38 2 158 232 72 144 4.97e-14 72.4
Msa0259580 AT2G37260.2 37.500 120 64 3 113 228 211 323 6.86e-16 77.8
Msa0259580 AT2G37260.2 46.667 75 38 2 158 232 70 142 5.10e-14 72.4
Msa0259580 AT5G28650.1 51.667 60 29 0 169 228 260 319 6.91e-16 77.4
Msa0259580 AT1G30650.1 45.714 70 38 0 164 233 210 279 7.63e-16 78.2
Msa0259580 AT2G37260.1 37.037 135 69 4 113 238 293 420 1.01e-15 77.8
Msa0259580 AT2G37260.1 46.667 75 38 2 158 232 152 224 5.50e-14 72.4
Msa0259580 AT2G24570.1 50.000 60 30 0 169 228 241 300 1.16e-15 76.6
Msa0259580 AT3G58710.2 40.323 124 64 3 129 245 24 144 2.23e-15 75.5
Msa0259580 AT4G23550.1 31.507 146 88 4 169 314 138 271 2.36e-15 75.9
Msa0259580 AT3G58710.1 40.000 125 64 3 129 245 24 145 3.21e-15 74.7
Msa0259580 AT4G30935.1 42.466 73 41 1 159 231 319 390 5.92e-15 75.5
Msa0259580 AT5G45050.2 35.833 120 68 2 172 282 1153 1272 1.38e-14 75.1
Msa0259580 AT5G45050.1 35.833 120 68 2 172 282 1181 1300 1.40e-14 75.1
Msa0259580 AT5G45050.4 47.541 61 32 0 172 232 1042 1102 1.50e-14 75.1
Msa0259580 AT2G46130.2 48.101 79 34 3 151 229 12 83 7.00e-14 67.4
Msa0259580 AT2G46400.1 41.096 73 39 2 156 226 91 161 7.13e-12 65.5
Msa0259580 AT5G45260.1 37.975 79 47 1 172 248 1209 1287 1.41e-11 65.9
Msa0259580 AT3G01080.3 41.333 75 42 2 172 246 206 278 4.40e-11 63.5

Find 66 sgRNAs with CRISPR-Local

Find 182 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATCTGTGGTACTTCCATTT+TGG 0.140306 2_2:+56623406 None:intergenic
AATGGAAGTACCACAGATTT+TGG 0.248540 2_2:-56623402 Msa0259580:CDS
TCATACTTCTTGAAGCTTAC+AGG 0.255823 2_2:+56624384 None:intergenic
GCACTTGCTGCTGCCATATC+AGG 0.283466 2_2:-56623332 Msa0259580:CDS
AATCTAATGGGAGGAGTAAC+TGG 0.300205 2_2:-56623994 Msa0259580:CDS
TATGTTGCAACCACAATTGA+TGG 0.331858 2_2:+56623599 None:intergenic
GGATATCAGTGGAGAAAATA+TGG 0.332364 2_2:-56623725 Msa0259580:CDS
TGAATAATTTATTTGACATA+TGG 0.338190 2_2:-56624481 Msa0259580:exon
AAGGTTCTACTGGACTAATT+TGG 0.339982 2_2:+56623513 None:intergenic
GATGATAAATAATCCCAAAA+TGG 0.343074 2_2:-56623420 Msa0259580:CDS
TTTATCATCATATTCTTAGA+AGG 0.347068 2_2:+56623434 None:intergenic
AATAGTAGAAACTTGCATTA+AGG 0.347618 2_2:-56623914 Msa0259580:CDS
CAAGTACAGTTACACTTGAT+TGG 0.350481 2_2:-56623481 Msa0259580:CDS
AAGCAGCAGTAACAATCTAA+TGG 0.359770 2_2:-56624007 Msa0259580:CDS
AAGTCAAATGTTCTATAAAC+AGG 0.392019 2_2:-56623264 Msa0259580:three_prime_UTR
TTCTTCTTCACTGTGCAGCT+TGG 0.402986 2_2:+56623644 None:intergenic
TTCCTACAACGATTGTTTGT+CGG 0.422479 2_2:+56623536 None:intergenic
CAAGTTTCTACTATTTGCCT+TGG 0.425526 2_2:+56623922 None:intergenic
GGGCTTTGCTCCTTATGATC+AGG 0.429712 2_2:+56624057 None:intergenic
GAGAAAGAATCCTGATCATA+AGG 0.436763 2_2:-56624067 Msa0259580:CDS
AGCAGCAGTAACAATCTAAT+GGG 0.440609 2_2:-56624006 Msa0259580:CDS
TCAGTCAAGTGGTGCCTTGG+TGG 0.446693 2_2:-56624193 Msa0259580:intron
TTGAAGTATGCTCTTGGAGA+TGG 0.459401 2_2:+56623680 None:intergenic
ATCATGGATTAACACTTCCT+TGG 0.460598 2_2:-56624448 Msa0259580:CDS
GTGGAGAAAATATGGACAAA+AGG 0.461765 2_2:-56623717 Msa0259580:CDS
AGCAAGGAAAAGAACTAGCT+TGG 0.467320 2_2:-56623076 Msa0259580:three_prime_UTR
GAGCATTTGAAGTATGCTCT+TGG 0.467728 2_2:+56623674 None:intergenic
CAATCAAGTGTAACTGTACT+TGG 0.475314 2_2:+56623482 None:intergenic
AGCAAAGCCCATCTCAGAAA+AGG 0.487615 2_2:-56624044 Msa0259580:CDS
GTCAAGTGGTGCCTTGGTGG+AGG 0.490012 2_2:-56624190 Msa0259580:CDS
CTACTTAATGAATCAAAATG+AGG 0.491460 2_2:+56623175 None:intergenic
TGCTCTGAAGTTAAGATCCT+TGG 0.493603 2_2:+56623355 None:intergenic
TTTGATTCATTAAGTAGGAA+AGG 0.506007 2_2:-56623169 Msa0259580:three_prime_UTR
TATATCTTCTTCATCAAGCA+AGG 0.508290 2_2:-56623092 Msa0259580:three_prime_UTR
TTCTTAGAAGGTGCGGAAGA+AGG 0.509300 2_2:+56623446 None:intergenic
TCATCCTCATCCTTCTCAAA+CGG 0.516116 2_2:-56623561 Msa0259580:CDS
GGTGCAGAAGAAGGTTCTAC+TGG 0.520924 2_2:+56623503 None:intergenic
GCAGAAAACAAGAAGTTGAC+AGG 0.521855 2_2:-56624150 Msa0259580:CDS
ATTGTGGTTGCAACATATGA+AGG 0.523365 2_2:-56623593 Msa0259580:CDS
ACTGTACTTGGTGCAGAAGA+AGG 0.524453 2_2:+56623494 None:intergenic
CACATTGAGAAACAATCTGA+TGG 0.530744 2_2:-56624097 Msa0259580:CDS
TGTAAGCTTCAAGAAGTATG+AGG 0.535043 2_2:-56624382 Msa0259580:CDS
CAACATTAATCCACGCAGTA+GGG 0.544477 2_2:-56624421 Msa0259580:CDS
TCAACATTAATCCACGCAGT+AGG 0.551260 2_2:-56624422 Msa0259580:CDS
AGCCGACAAACAATCGTTGT+AGG 0.557166 2_2:-56623538 Msa0259580:CDS
TATATCTTCTGCAAAAGAAG+AGG 0.565925 2_2:-56624328 Msa0259580:intron
TTTGTCGGCTCCGTTTGAGA+AGG 0.566050 2_2:+56623551 None:intergenic
CATCATATTCTTAGAAGGTG+CGG 0.576775 2_2:+56623439 None:intergenic
GGAAAAGAACTAGCTTGGAT+AGG 0.582825 2_2:-56623071 Msa0259580:three_prime_UTR
AAATACAACATAGAGAAAGT+AGG 0.586245 2_2:-56623204 Msa0259580:three_prime_UTR
TGGATTAATGTTGAGATCCA+AGG 0.586656 2_2:+56624431 None:intergenic
GAAGAAGGTGCAAAGAAGTG+TGG 0.601905 2_2:-56623627 Msa0259580:CDS
ATATGGACAAAAGGTGACAA+GGG 0.608161 2_2:-56623708 Msa0259580:CDS
ATCAATCATGCAAGAAACCA+AGG 0.612418 2_2:-56623939 Msa0259580:CDS
TACTTCTTGAAGCTTACAGG+AGG 0.621579 2_2:+56624387 None:intergenic
AATATGGACAAAAGGTGACA+AGG 0.623618 2_2:-56623709 Msa0259580:CDS
ATCTGTTCCACCAAAATCTG+TGG 0.633715 2_2:+56623392 None:intergenic
GGCTCCGTTTGAGAAGGATG+AGG 0.643319 2_2:+56623557 None:intergenic
AAGCTTCAAGAAGTATGAGG+TGG 0.647098 2_2:-56624379 Msa0259580:CDS
TTGTGAAAGATGGATATCAG+TGG 0.648462 2_2:-56623736 Msa0259580:CDS
AGCAGTAACAATCTAATGGG+AGG 0.658927 2_2:-56624003 Msa0259580:CDS
TGTGGATGATCCATCAATTG+TGG 0.662432 2_2:-56623609 Msa0259580:CDS
AAGCTGCACAGTGAAGAAGA+AGG 0.665099 2_2:-56623642 Msa0259580:CDS
GATGGCTACTTCATTGACCA+AGG 0.672567 2_2:-56623372 Msa0259580:CDS
TGTTCATGAACCCTACTGCG+TGG 0.674456 2_2:+56624411 None:intergenic
TCGTTTCAGCTCCTCCACCA+AGG 0.688042 2_2:+56624179 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGAATAATTTATTTGACATA+TGG - chr2_2:56623095-56623114 Msa0259580:three_prime_UTR 15.0%
!!! AAAACAATTTTTTCTCATTA+GGG - chr2_2:56623220-56623239 Msa0259580:three_prime_UTR 15.0%
!! TGAATAATTTATTTGACATA+TGG - chr2_2:56623095-56623114 Msa0259580:three_prime_UTR 15.0%
!!! AAAACAATTTTTTCTCATTA+GGG - chr2_2:56623220-56623239 Msa0259580:three_prime_UTR 15.0%
!!! GAAAACAATTTTTTCTCATT+AGG - chr2_2:56623219-56623238 Msa0259580:three_prime_UTR 20.0%
!! TTATGAATACTAGATATATG+AGG - chr2_2:56623313-56623332 Msa0259580:CDS 20.0%
!! TTTATCATCATATTCTTAGA+AGG + chr2_2:56624145-56624164 None:intergenic 20.0%
!!! TTTTCTACTTGAACTAATTT+TGG + chr2_2:56624282-56624301 None:intergenic 20.0%
!! TTTGCAAAAACATAAACAAT+TGG + chr2_2:56624443-56624462 None:intergenic 20.0%
!!! GAAAACAATTTTTTCTCATT+AGG - chr2_2:56623219-56623238 Msa0259580:three_prime_UTR 20.0%
!! TTATGAATACTAGATATATG+AGG - chr2_2:56623313-56623332 Msa0259580:CDS 20.0%
!! TTTATCATCATATTCTTAGA+AGG + chr2_2:56624145-56624164 None:intergenic 20.0%
!!! TTTTCTACTTGAACTAATTT+TGG + chr2_2:56624282-56624301 None:intergenic 20.0%
!! TTTGCAAAAACATAAACAAT+TGG + chr2_2:56624443-56624462 None:intergenic 20.0%
!! ATATGGATTTTTCATCATCA+TGG - chr2_2:56623112-56623131 Msa0259580:three_prime_UTR 25.0%
!! TACTAGATATATGAGGTTTT+TGG - chr2_2:56623320-56623339 Msa0259580:CDS 25.0%
! AATAGTAGAAACTTGCATTA+AGG - chr2_2:56623662-56623681 Msa0259580:CDS 25.0%
! GAAGAAAAATGAATCTTACA+AGG + chr2_2:56623734-56623753 None:intergenic 25.0%
!! TATTTTCAGATTGTGAAAGA+TGG - chr2_2:56623830-56623849 Msa0259580:intron 25.0%
! GATGATAAATAATCCCAAAA+TGG - chr2_2:56624156-56624175 Msa0259580:CDS 25.0%
! AAGTCAAATGTTCTATAAAC+AGG - chr2_2:56624312-56624331 Msa0259580:intron 25.0%
!!! AACAGGTCATTTTTAAAACA+TGG - chr2_2:56624329-56624348 Msa0259580:CDS 25.0%
!!! TCATTTTTAAAACATGGCAT+AGG - chr2_2:56624335-56624354 Msa0259580:CDS 25.0%
! AAATACAACATAGAGAAAGT+AGG - chr2_2:56624372-56624391 Msa0259580:CDS 25.0%
! CTACTTAATGAATCAAAATG+AGG + chr2_2:56624404-56624423 None:intergenic 25.0%
!!! CTCATTTTGATTCATTAAGT+AGG - chr2_2:56624402-56624421 Msa0259580:CDS 25.0%
! TTTGATTCATTAAGTAGGAA+AGG - chr2_2:56624407-56624426 Msa0259580:CDS 25.0%
!!! TGTTTATGTTTTTGCAAATC+TGG - chr2_2:56624445-56624464 Msa0259580:CDS 25.0%
!! ATATGGATTTTTCATCATCA+TGG - chr2_2:56623112-56623131 Msa0259580:three_prime_UTR 25.0%
!! TACTAGATATATGAGGTTTT+TGG - chr2_2:56623320-56623339 Msa0259580:CDS 25.0%
! AATAGTAGAAACTTGCATTA+AGG - chr2_2:56623662-56623681 Msa0259580:CDS 25.0%
! GAAGAAAAATGAATCTTACA+AGG + chr2_2:56623734-56623753 None:intergenic 25.0%
!! TATTTTCAGATTGTGAAAGA+TGG - chr2_2:56623830-56623849 Msa0259580:intron 25.0%
! GATGATAAATAATCCCAAAA+TGG - chr2_2:56624156-56624175 Msa0259580:CDS 25.0%
! AAGTCAAATGTTCTATAAAC+AGG - chr2_2:56624312-56624331 Msa0259580:intron 25.0%
!!! AACAGGTCATTTTTAAAACA+TGG - chr2_2:56624329-56624348 Msa0259580:CDS 25.0%
!!! TCATTTTTAAAACATGGCAT+AGG - chr2_2:56624335-56624354 Msa0259580:CDS 25.0%
! AAATACAACATAGAGAAAGT+AGG - chr2_2:56624372-56624391 Msa0259580:CDS 25.0%
! CTACTTAATGAATCAAAATG+AGG + chr2_2:56624404-56624423 None:intergenic 25.0%
!!! CTCATTTTGATTCATTAAGT+AGG - chr2_2:56624402-56624421 Msa0259580:CDS 25.0%
! TTTGATTCATTAAGTAGGAA+AGG - chr2_2:56624407-56624426 Msa0259580:CDS 25.0%
!!! TGTTTATGTTTTTGCAAATC+TGG - chr2_2:56624445-56624464 Msa0259580:CDS 25.0%
TATATCTTCTGCAAAAGAAG+AGG - chr2_2:56623248-56623267 Msa0259580:three_prime_UTR 30.0%
AAAGAAGAGGTAAGTTAGTT+TGG - chr2_2:56623261-56623280 Msa0259580:three_prime_UTR 30.0%
! GTAAGTTAGTTTGGTTACTT+TGG - chr2_2:56623270-56623289 Msa0259580:three_prime_UTR 30.0%
! GATTGATATTTTCAGTCAAG+TGG - chr2_2:56623372-56623391 Msa0259580:CDS 30.0%
ATTTGCAAAGCCATTTCATT+AGG - chr2_2:56623761-56623780 Msa0259580:intron 30.0%
AGTCATACAACCTAATGAAA+TGG + chr2_2:56623774-56623793 None:intergenic 30.0%
! GTGTAACATTTTTCCTGATA+TGG + chr2_2:56624260-56624279 None:intergenic 30.0%
TATATCTTCTTCATCAAGCA+AGG - chr2_2:56624484-56624503 Msa0259580:exon 30.0%
TATATCTTCTGCAAAAGAAG+AGG - chr2_2:56623248-56623267 Msa0259580:three_prime_UTR 30.0%
AAAGAAGAGGTAAGTTAGTT+TGG - chr2_2:56623261-56623280 Msa0259580:three_prime_UTR 30.0%
! GTAAGTTAGTTTGGTTACTT+TGG - chr2_2:56623270-56623289 Msa0259580:three_prime_UTR 30.0%
! GATTGATATTTTCAGTCAAG+TGG - chr2_2:56623372-56623391 Msa0259580:CDS 30.0%
ATTTGCAAAGCCATTTCATT+AGG - chr2_2:56623761-56623780 Msa0259580:intron 30.0%
AGTCATACAACCTAATGAAA+TGG + chr2_2:56623774-56623793 None:intergenic 30.0%
! GTGTAACATTTTTCCTGATA+TGG + chr2_2:56624260-56624279 None:intergenic 30.0%
TATATCTTCTTCATCAAGCA+AGG - chr2_2:56624484-56624503 Msa0259580:exon 30.0%
! ATCATGGATTAACACTTCCT+TGG - chr2_2:56623128-56623147 Msa0259580:three_prime_UTR 35.0%
TGGATTAATGTTGAGATCCA+AGG + chr2_2:56623148-56623167 None:intergenic 35.0%
TCATACTTCTTGAAGCTTAC+AGG + chr2_2:56623195-56623214 None:intergenic 35.0%
! TGTAAGCTTCAAGAAGTATG+AGG - chr2_2:56623194-56623213 Msa0259580:three_prime_UTR 35.0%
CACATTGAGAAACAATCTGA+TGG - chr2_2:56623479-56623498 Msa0259580:CDS 35.0%
GAGAAAGAATCCTGATCATA+AGG - chr2_2:56623509-56623528 Msa0259580:CDS 35.0%
!!! CAGATTTCCTTTTCTGAGAT+GGG + chr2_2:56623542-56623561 None:intergenic 35.0%
!! TCAGATTTCCTTTTCTGAGA+TGG + chr2_2:56623543-56623562 None:intergenic 35.0%
AAGCAGCAGTAACAATCTAA+TGG - chr2_2:56623569-56623588 Msa0259580:CDS 35.0%
AGCAGCAGTAACAATCTAAT+GGG - chr2_2:56623570-56623589 Msa0259580:CDS 35.0%
ATCAATCATGCAAGAAACCA+AGG - chr2_2:56623637-56623656 Msa0259580:CDS 35.0%
CAAGTTTCTACTATTTGCCT+TGG + chr2_2:56623657-56623676 None:intergenic 35.0%
TTGTGAAAGATGGATATCAG+TGG - chr2_2:56623840-56623859 Msa0259580:intron 35.0%
GGATATCAGTGGAGAAAATA+TGG - chr2_2:56623851-56623870 Msa0259580:intron 35.0%
GTGGAGAAAATATGGACAAA+AGG - chr2_2:56623859-56623878 Msa0259580:intron 35.0%
AATATGGACAAAAGGTGACA+AGG - chr2_2:56623867-56623886 Msa0259580:CDS 35.0%
ATATGGACAAAAGGTGACAA+GGG - chr2_2:56623868-56623887 Msa0259580:CDS 35.0%
TATGTTGCAACCACAATTGA+TGG + chr2_2:56623980-56623999 None:intergenic 35.0%
ATTGTGGTTGCAACATATGA+AGG - chr2_2:56623983-56624002 Msa0259580:CDS 35.0%
TTCCTACAACGATTGTTTGT+CGG + chr2_2:56624043-56624062 None:intergenic 35.0%
AAGGTTCTACTGGACTAATT+TGG + chr2_2:56624066-56624085 None:intergenic 35.0%
CAATCAAGTGTAACTGTACT+TGG + chr2_2:56624097-56624116 None:intergenic 35.0%
CAAGTACAGTTACACTTGAT+TGG - chr2_2:56624095-56624114 Msa0259580:CDS 35.0%
CATCATATTCTTAGAAGGTG+CGG + chr2_2:56624140-56624159 None:intergenic 35.0%
! ATCTGTGGTACTTCCATTTT+GGG + chr2_2:56624172-56624191 None:intergenic 35.0%
! AATCTGTGGTACTTCCATTT+TGG + chr2_2:56624173-56624192 None:intergenic 35.0%
!!! AATGGAAGTACCACAGATTT+TGG - chr2_2:56624174-56624193 Msa0259580:CDS 35.0%
! ATCATGGATTAACACTTCCT+TGG - chr2_2:56623128-56623147 Msa0259580:three_prime_UTR 35.0%
TGGATTAATGTTGAGATCCA+AGG + chr2_2:56623148-56623167 None:intergenic 35.0%
TCATACTTCTTGAAGCTTAC+AGG + chr2_2:56623195-56623214 None:intergenic 35.0%
! TGTAAGCTTCAAGAAGTATG+AGG - chr2_2:56623194-56623213 Msa0259580:three_prime_UTR 35.0%
CACATTGAGAAACAATCTGA+TGG - chr2_2:56623479-56623498 Msa0259580:CDS 35.0%
GAGAAAGAATCCTGATCATA+AGG - chr2_2:56623509-56623528 Msa0259580:CDS 35.0%
!!! CAGATTTCCTTTTCTGAGAT+GGG + chr2_2:56623542-56623561 None:intergenic 35.0%
!! TCAGATTTCCTTTTCTGAGA+TGG + chr2_2:56623543-56623562 None:intergenic 35.0%
AAGCAGCAGTAACAATCTAA+TGG - chr2_2:56623569-56623588 Msa0259580:CDS 35.0%
AGCAGCAGTAACAATCTAAT+GGG - chr2_2:56623570-56623589 Msa0259580:CDS 35.0%
ATCAATCATGCAAGAAACCA+AGG - chr2_2:56623637-56623656 Msa0259580:CDS 35.0%
CAAGTTTCTACTATTTGCCT+TGG + chr2_2:56623657-56623676 None:intergenic 35.0%
TTGTGAAAGATGGATATCAG+TGG - chr2_2:56623840-56623859 Msa0259580:intron 35.0%
GGATATCAGTGGAGAAAATA+TGG - chr2_2:56623851-56623870 Msa0259580:intron 35.0%
GTGGAGAAAATATGGACAAA+AGG - chr2_2:56623859-56623878 Msa0259580:intron 35.0%
AATATGGACAAAAGGTGACA+AGG - chr2_2:56623867-56623886 Msa0259580:CDS 35.0%
ATATGGACAAAAGGTGACAA+GGG - chr2_2:56623868-56623887 Msa0259580:CDS 35.0%
TATGTTGCAACCACAATTGA+TGG + chr2_2:56623980-56623999 None:intergenic 35.0%
ATTGTGGTTGCAACATATGA+AGG - chr2_2:56623983-56624002 Msa0259580:CDS 35.0%
TTCCTACAACGATTGTTTGT+CGG + chr2_2:56624043-56624062 None:intergenic 35.0%
AAGGTTCTACTGGACTAATT+TGG + chr2_2:56624066-56624085 None:intergenic 35.0%
CAATCAAGTGTAACTGTACT+TGG + chr2_2:56624097-56624116 None:intergenic 35.0%
CAAGTACAGTTACACTTGAT+TGG - chr2_2:56624095-56624114 Msa0259580:CDS 35.0%
CATCATATTCTTAGAAGGTG+CGG + chr2_2:56624140-56624159 None:intergenic 35.0%
! ATCTGTGGTACTTCCATTTT+GGG + chr2_2:56624172-56624191 None:intergenic 35.0%
! AATCTGTGGTACTTCCATTT+TGG + chr2_2:56624173-56624192 None:intergenic 35.0%
!!! AATGGAAGTACCACAGATTT+TGG - chr2_2:56624174-56624193 Msa0259580:CDS 35.0%
TCAACATTAATCCACGCAGT+AGG - chr2_2:56623154-56623173 Msa0259580:three_prime_UTR 40.0%
CAACATTAATCCACGCAGTA+GGG - chr2_2:56623155-56623174 Msa0259580:three_prime_UTR 40.0%
TACTTCTTGAAGCTTACAGG+AGG + chr2_2:56623192-56623211 None:intergenic 40.0%
! AAGCTTCAAGAAGTATGAGG+TGG - chr2_2:56623197-56623216 Msa0259580:three_prime_UTR 40.0%
GCAGAAAACAAGAAGTTGAC+AGG - chr2_2:56623426-56623445 Msa0259580:CDS 40.0%
AGCAGTAACAATCTAATGGG+AGG - chr2_2:56623573-56623592 Msa0259580:CDS 40.0%
AATCTAATGGGAGGAGTAAC+TGG - chr2_2:56623582-56623601 Msa0259580:CDS 40.0%
TTGAAGTATGCTCTTGGAGA+TGG + chr2_2:56623899-56623918 None:intergenic 40.0%
! GAGCATTTGAAGTATGCTCT+TGG + chr2_2:56623905-56623924 None:intergenic 40.0%
TGTGGATGATCCATCAATTG+TGG - chr2_2:56623967-56623986 Msa0259580:CDS 40.0%
TCATCCTCATCCTTCTCAAA+CGG - chr2_2:56624015-56624034 Msa0259580:CDS 40.0%
ATCTGTTCCACCAAAATCTG+TGG + chr2_2:56624187-56624206 None:intergenic 40.0%
!! ACAGATTTTGGTGGAACAGA+TGG - chr2_2:56624186-56624205 Msa0259580:CDS 40.0%
!! TGCTCTGAAGTTAAGATCCT+TGG + chr2_2:56624224-56624243 None:intergenic 40.0%
AGCAAGGAAAAGAACTAGCT+TGG - chr2_2:56624500-56624519 Msa0259580:five_prime_UTR 40.0%
! GGAAAAGAACTAGCTTGGAT+AGG - chr2_2:56624505-56624524 Msa0259580:five_prime_UTR 40.0%
TCAACATTAATCCACGCAGT+AGG - chr2_2:56623154-56623173 Msa0259580:three_prime_UTR 40.0%
CAACATTAATCCACGCAGTA+GGG - chr2_2:56623155-56623174 Msa0259580:three_prime_UTR 40.0%
TACTTCTTGAAGCTTACAGG+AGG + chr2_2:56623192-56623211 None:intergenic 40.0%
! AAGCTTCAAGAAGTATGAGG+TGG - chr2_2:56623197-56623216 Msa0259580:three_prime_UTR 40.0%
GCAGAAAACAAGAAGTTGAC+AGG - chr2_2:56623426-56623445 Msa0259580:CDS 40.0%
AGCAGTAACAATCTAATGGG+AGG - chr2_2:56623573-56623592 Msa0259580:CDS 40.0%
AATCTAATGGGAGGAGTAAC+TGG - chr2_2:56623582-56623601 Msa0259580:CDS 40.0%
TTGAAGTATGCTCTTGGAGA+TGG + chr2_2:56623899-56623918 None:intergenic 40.0%
! GAGCATTTGAAGTATGCTCT+TGG + chr2_2:56623905-56623924 None:intergenic 40.0%
TGTGGATGATCCATCAATTG+TGG - chr2_2:56623967-56623986 Msa0259580:CDS 40.0%
TCATCCTCATCCTTCTCAAA+CGG - chr2_2:56624015-56624034 Msa0259580:CDS 40.0%
ATCTGTTCCACCAAAATCTG+TGG + chr2_2:56624187-56624206 None:intergenic 40.0%
!! ACAGATTTTGGTGGAACAGA+TGG - chr2_2:56624186-56624205 Msa0259580:CDS 40.0%
!! TGCTCTGAAGTTAAGATCCT+TGG + chr2_2:56624224-56624243 None:intergenic 40.0%
AGCAAGGAAAAGAACTAGCT+TGG - chr2_2:56624500-56624519 Msa0259580:five_prime_UTR 40.0%
! GGAAAAGAACTAGCTTGGAT+AGG - chr2_2:56624505-56624524 Msa0259580:five_prime_UTR 40.0%
! TTTTCAGTCAAGTGGTGCCT+TGG - chr2_2:56623380-56623399 Msa0259580:CDS 45.0%
AGCAAAGCCCATCTCAGAAA+AGG - chr2_2:56623532-56623551 Msa0259580:CDS 45.0%
TTCTTCTTCACTGTGCAGCT+TGG + chr2_2:56623935-56623954 None:intergenic 45.0%
AAGCTGCACAGTGAAGAAGA+AGG - chr2_2:56623934-56623953 Msa0259580:CDS 45.0%
! GAAGAAGGTGCAAAGAAGTG+TGG - chr2_2:56623949-56623968 Msa0259580:CDS 45.0%
AGCCGACAAACAATCGTTGT+AGG - chr2_2:56624038-56624057 Msa0259580:CDS 45.0%
!! ACTGTACTTGGTGCAGAAGA+AGG + chr2_2:56624085-56624104 None:intergenic 45.0%
! TTCTTAGAAGGTGCGGAAGA+AGG + chr2_2:56624133-56624152 None:intergenic 45.0%
!!! GGAAGTACCACAGATTTTGG+TGG - chr2_2:56624177-56624196 Msa0259580:CDS 45.0%
GATGGCTACTTCATTGACCA+AGG - chr2_2:56624204-56624223 Msa0259580:intron 45.0%
! TTTTCAGTCAAGTGGTGCCT+TGG - chr2_2:56623380-56623399 Msa0259580:CDS 45.0%
AGCAAAGCCCATCTCAGAAA+AGG - chr2_2:56623532-56623551 Msa0259580:CDS 45.0%
TTCTTCTTCACTGTGCAGCT+TGG + chr2_2:56623935-56623954 None:intergenic 45.0%
AAGCTGCACAGTGAAGAAGA+AGG - chr2_2:56623934-56623953 Msa0259580:CDS 45.0%
! GAAGAAGGTGCAAAGAAGTG+TGG - chr2_2:56623949-56623968 Msa0259580:CDS 45.0%
AGCCGACAAACAATCGTTGT+AGG - chr2_2:56624038-56624057 Msa0259580:CDS 45.0%
!! ACTGTACTTGGTGCAGAAGA+AGG + chr2_2:56624085-56624104 None:intergenic 45.0%
! TTCTTAGAAGGTGCGGAAGA+AGG + chr2_2:56624133-56624152 None:intergenic 45.0%
!!! GGAAGTACCACAGATTTTGG+TGG - chr2_2:56624177-56624196 Msa0259580:CDS 45.0%
GATGGCTACTTCATTGACCA+AGG - chr2_2:56624204-56624223 Msa0259580:intron 45.0%
TGTTCATGAACCCTACTGCG+TGG + chr2_2:56623168-56623187 None:intergenic 50.0%
GGGCTTTGCTCCTTATGATC+AGG + chr2_2:56623522-56623541 None:intergenic 50.0%
!! TTTGTCGGCTCCGTTTGAGA+AGG + chr2_2:56624028-56624047 None:intergenic 50.0%
GGTGCAGAAGAAGGTTCTAC+TGG + chr2_2:56624076-56624095 None:intergenic 50.0%
TGTTCATGAACCCTACTGCG+TGG + chr2_2:56623168-56623187 None:intergenic 50.0%
GGGCTTTGCTCCTTATGATC+AGG + chr2_2:56623522-56623541 None:intergenic 50.0%
!! TTTGTCGGCTCCGTTTGAGA+AGG + chr2_2:56624028-56624047 None:intergenic 50.0%
GGTGCAGAAGAAGGTTCTAC+TGG + chr2_2:56624076-56624095 None:intergenic 50.0%
TCAGTCAAGTGGTGCCTTGG+TGG - chr2_2:56623383-56623402 Msa0259580:CDS 55.0%
TCGTTTCAGCTCCTCCACCA+AGG + chr2_2:56623400-56623419 None:intergenic 55.0%
GGCTCCGTTTGAGAAGGATG+AGG + chr2_2:56624022-56624041 None:intergenic 55.0%
! GCACTTGCTGCTGCCATATC+AGG - chr2_2:56624244-56624263 Msa0259580:intron 55.0%
TCAGTCAAGTGGTGCCTTGG+TGG - chr2_2:56623383-56623402 Msa0259580:CDS 55.0%
TCGTTTCAGCTCCTCCACCA+AGG + chr2_2:56623400-56623419 None:intergenic 55.0%
GGCTCCGTTTGAGAAGGATG+AGG + chr2_2:56624022-56624041 None:intergenic 55.0%
! GCACTTGCTGCTGCCATATC+AGG - chr2_2:56624244-56624263 Msa0259580:intron 55.0%
GTCAAGTGGTGCCTTGGTGG+AGG - chr2_2:56623386-56623405 Msa0259580:CDS 60.0%
GTCAAGTGGTGCCTTGGTGG+AGG - chr2_2:56623386-56623405 Msa0259580:CDS 60.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 56623030 56624568 56623030 ID=Msa0259580;Name=Msa0259580
chr2_2 mRNA 56623030 56624568 56623030 ID=Msa0259580-mRNA-1;Parent=Msa0259580;Name=Msa0259580-mRNA-1;_AED=0.07;_eAED=0.07;_QI=84|1|1|1|1|1|3|280|317
chr2_2 exon 56623030 56623759 56623030 ID=Msa0259580-mRNA-1:exon:17195;Parent=Msa0259580-mRNA-1
chr2_2 exon 56623864 56624211 56623864 ID=Msa0259580-mRNA-1:exon:17194;Parent=Msa0259580-mRNA-1
chr2_2 exon 56624329 56624568 56624329 ID=Msa0259580-mRNA-1:exon:17193;Parent=Msa0259580-mRNA-1
chr2_2 five_prime_UTR 56624485 56624568 56624485 ID=Msa0259580-mRNA-1:five_prime_utr;Parent=Msa0259580-mRNA-1
chr2_2 CDS 56624329 56624484 56624329 ID=Msa0259580-mRNA-1:cds;Parent=Msa0259580-mRNA-1
chr2_2 CDS 56623864 56624211 56623864 ID=Msa0259580-mRNA-1:cds;Parent=Msa0259580-mRNA-1
chr2_2 CDS 56623310 56623759 56623310 ID=Msa0259580-mRNA-1:cds;Parent=Msa0259580-mRNA-1
chr2_2 three_prime_UTR 56623030 56623309 56623030 ID=Msa0259580-mRNA-1:three_prime_utr;Parent=Msa0259580-mRNA-1
Gene Sequence

>Msa0259580

ATGGATTTTTCATCATCATGGATTAACACTTCCTTGGATCTCAACATTAATCCACGCAGTAGGGTTCATGAACAACCTCCTGTAAGCTTCAAGAAGTATGAGGTGGAAAACAATTTTTTCTCATTAGGGATGAGTATATCTTCTGCAAAAGAAGAGTCAAGTGGTGCCTTGGTGGAGGAGCTGAAACGAGTGAGTGCAGAAAACAAGAAGTTGACAGGAATGTTGACTGAGATGTGTGAGAATTACAACACATTGAGAAACAATCTGATGGAATATATGAGAAAGAATCCTGATCATAAGGAGCAAAGCCCATCTCAGAAAAGGAAATCTGATGAAATAAGCAGCAGTAACAATCTAATGGGAGGAGTAACTGGAAACAACTCTGAGAGCAGTTCAAGTGATGAAGATCAATCATGCAAGAAACCAAGGCAAATAGTAGAAACTTGCATTAAGGCTAAAGTTTCAAGAGTTTATTTCAAGACAGAATCATCTGATTCAAGCCTTATTGTGAAAGATGGATATCAGTGGAGAAAATATGGACAAAAGGTGACAAGGGATAATCCATCTCCAAGAGCATACTTCAAATGCTCATTTGCTCCAAGCTGCACAGTGAAGAAGAAGGTGCAAAGAAGTGTGGATGATCCATCAATTGTGGTTGCAACATATGAAGGAGAGCATAATCATCCTCATCCTTCTCAAACGGAGCCGACAAACAATCGTTGTAGGAACCAAATTAGTCCAGTAGAACCTTCTTCTGCACCAAGTACAGTTACACTTGATTGGACAAAGAAAGCACCTTCTTCCGCACCTTCTAAGAATATGATGATAAATAATCCCAAAATGGAAGTACCACAGATTTTGGTGGAACAGATGGCTACTTCATTGACCAAGGATCTTAACTTCAGAGCAGCACTTGCTGCTGCCATATCAGGAAAAATGTTACACCAAAATTAG
Protein sequence

>Msa0259580

MDFSSSWINTSLDLNINPRSRVHEQPPVSFKKYEVENNFFSLGMSISSAKEESSGALVEELKRVSAENKKLTGMLTEMCENYNTLRNNLMEYMRKNPDHKEQSPSQKRKSDEISSSNNLMGGVTGNNSESSSSDEDQSCKKPRQIVETCIKAKVSRVYFKTESSDSSLIVKDGYQWRKYGQKVTRDNPSPRAYFKCSFAPSCTVKKKVQRSVDDPSIVVATYEGEHNHPHPSQTEPTNNRCRNQISPVEPSSAPSTVTLDWTKKAPSSAPSKNMMINNPKMEVPQILVEQMATSLTKDLNFRAALAAAISGKMLHQN*