AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0686010

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0686010 MtrunA17_Chr4g0034611 97.941 437 9 0 1 437 1 437 0.0 890
Msa0686010 MtrunA17_Chr4g0034621 84.703 438 66 1 1 437 1 438 0.0 763
Msa0686010 MtrunA17_Chr4g0034641 80.505 436 79 2 1 435 1 431 0.0 724
Msa0686010 MtrunA17_Chr4g0034631 80.963 436 75 2 1 435 1 429 0.0 721
Msa0686010 MtrunA17_Chr4g0034651 79.310 435 82 1 1 435 34 460 0.0 698
Msa0686010 MtrunA17_Chr4g0034661 80.000 435 72 2 1 435 1 420 0.0 690
Msa0686010 MtrunA17_Chr1g0154701 73.624 436 107 2 1 436 1 428 0.0 673
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0686010 AT1G19360.1 72.540 437 110 3 1 436 1 428 0.0 654
Msa0686010 AT1G19360.2 72.540 437 110 3 1 436 1 428 0.0 654
Msa0686010 AT1G75110.1 71.101 436 118 1 1 436 1 428 0.0 647
Msa0686010 AT1G75120.1 60.920 435 137 4 1 435 1 402 0.0 537
Msa0686010 AT1G70630.5 28.169 213 122 6 148 354 231 418 8.44e-13 70.5
Msa0686010 AT1G70630.2 28.169 213 122 6 148 354 262 449 1.04e-12 70.5
Msa0686010 AT1G70630.1 28.169 213 122 6 148 354 262 449 1.04e-12 70.5
Msa0686010 AT1G70630.4 28.169 213 122 6 148 354 447 634 1.06e-12 70.5
Msa0686010 AT1G70630.3 28.169 213 122 6 148 354 478 665 1.23e-12 70.5
Msa0686010 AT1G70630.6 28.169 213 122 6 148 354 468 655 1.34e-12 70.5
Msa0686010 AT1G70630.7 28.169 213 122 6 148 354 399 586 1.39e-12 70.1

Find 117 sgRNAs with CRISPR-Local

Find 215 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTCCTTCAATTGAACTTT+TGG 0.197156 4_4:-50324492 Msa0686010:CDS
ATGAGGATTGTTTCGCATTA+AGG 0.231351 4_4:+50326719 None:intergenic
TCCAATATCTTTGCCAGTCT+TGG 0.235716 4_4:+50325013 None:intergenic
TGAAAATAAAACCTGGGTTT+TGG 0.240774 4_4:+50326560 None:intergenic
TTCATTCGCGGAAGCTTATC+AGG 0.249563 4_4:+50324248 None:intergenic
AGATGGTAGTGTTGTTCTTC+TGG 0.271544 4_4:+50326801 None:intergenic
AATTTGATATCAAGAATATT+TGG 0.299533 4_4:-50326850 Msa0686010:five_prime_UTR
TATACCAACTCTCTTGATAT+TGG 0.305862 4_4:+50324913 None:intergenic
CGTAAGCTGTCATGTTATTA+TGG 0.320845 4_4:+50324618 None:intergenic
AGGATATGGGTTTACAATTC+CGG 0.324078 4_4:-50324536 Msa0686010:CDS
GTTGTGATTGGAGTTCTTAT+TGG 0.330486 4_4:-50326666 Msa0686010:CDS
CTCTGCAAGCCGAAGCCTTT+CGG 0.335447 4_4:+50325159 None:intergenic
ACGCTCATACCATGAGGATA+TGG 0.335819 4_4:-50324550 Msa0686010:CDS
CCCAAGACTGGCAAAGATAT+TGG 0.338770 4_4:-50325014 Msa0686010:CDS
CCAATATCTTTGCCAGTCTT+GGG 0.362824 4_4:+50325014 None:intergenic
TAATTCACGTGAACAATTAC+CGG 0.366362 4_4:+50324275 None:intergenic
AAAATAAACAAAGAGGGAAA+GGG 0.384297 4_4:+50324007 None:intergenic
TCATGCTGCTAGAAGAACTA+TGG 0.394162 4_4:-50324372 Msa0686010:CDS
CGACGAACCTGCGATGGGTT+GGG 0.394380 4_4:-50324579 Msa0686010:CDS
TGAGGATTGTTTCGCATTAA+GGG 0.394783 4_4:+50326720 None:intergenic
CCATCTCAATTCAGTGTTTG+AGG 0.397352 4_4:-50326784 Msa0686010:five_prime_UTR
GATGATGGTATTGACACTAT+TGG 0.398863 4_4:-50324806 Msa0686010:CDS
GGAGGGAACCATGCTGTCTC+CGG 0.406427 4_4:-50324776 Msa0686010:CDS
GTTGTTGAATATTATGTCAA+TGG 0.415566 4_4:-50324221 Msa0686010:CDS
CAATTCAGTGTTTGAGGTGG+TGG 0.433911 4_4:-50326778 Msa0686010:five_prime_UTR
AACATGGATGCGACGATTTG+AGG 0.434841 4_4:+50326590 None:intergenic
TCGACGAACCTGCGATGGGT+TGG 0.436451 4_4:-50324580 Msa0686010:CDS
GGGAGTTGACCGAAAGGCTT+CGG 0.438259 4_4:-50325168 Msa0686010:CDS
GAAAATAAACAAAGAGGGAA+AGG 0.440174 4_4:+50324006 None:intergenic
ATAGTGTCAATACCATCATC+TGG 0.444142 4_4:+50324809 None:intergenic
ATACAATGTCAACGTCTGAT+AGG 0.444574 4_4:+50324702 None:intergenic
GGTTACCAATCTGAGCCATC+AGG 0.444586 4_4:+50324173 None:intergenic
CCGTCACACCCTGGTTATGA+AGG 0.446141 4_4:-50324398 Msa0686010:CDS
ATTGTTACCGCTGTTGTGAT+TGG 0.448923 4_4:-50326678 Msa0686010:CDS
GTTATTATGGCCATCGCTCA+TGG 0.461921 4_4:+50324631 None:intergenic
CATAATAACATGACAGCTTA+CGG 0.462428 4_4:-50324617 Msa0686010:CDS
AATCGTCGCATCCATGTTGT+TGG 0.462816 4_4:-50326585 Msa0686010:CDS
CTCATTCTCTACGCAAATCA+AGG 0.468828 4_4:-50326701 Msa0686010:CDS
CAAAAGTTCAATTGAAGGAA+TGG 0.471345 4_4:+50324493 None:intergenic
CATGTTAAAATCAGAGTTTG+TGG 0.477132 4_4:-50325230 Msa0686010:CDS
TTGTTGAATATTATGTCAAT+GGG 0.480177 4_4:-50324220 Msa0686010:CDS
AAAGAGTTCCTCGTTGAAAA+CGG 0.480750 4_4:+50324424 None:intergenic
CTTTCCCTGATGGCTCAGAT+TGG 0.480932 4_4:-50324178 Msa0686010:CDS
CTTGATTTGCGTAGAGAATG+AGG 0.482124 4_4:+50326702 None:intergenic
TAAAGGAGATGCTCGAGGTC+TGG 0.483638 4_4:-50324940 Msa0686010:CDS
TCAACAACTGCTTTCATTCG+CGG 0.485251 4_4:+50324236 None:intergenic
TTGGTCCCATGCGACCTCCT+TGG 0.491130 4_4:+50324448 None:intergenic
AAATAAACAAAGAGGGAAAG+GGG 0.492049 4_4:+50324008 None:intergenic
CGCTCATACCATGAGGATAT+GGG 0.494439 4_4:-50324549 Msa0686010:CDS
TGGTCTGATATAGAAGAAAC+CGG 0.496586 4_4:+50324517 None:intergenic
TACAAAAGAGACCCAGATGA+TGG 0.498054 4_4:-50324821 Msa0686010:CDS
ACTTGCAAATTCCAATGTAA+AGG 0.500585 4_4:-50324957 Msa0686010:CDS
TGAGAAACAAAGAAGCCATT+GGG 0.504608 4_4:+50326621 None:intergenic
ATCGGGAACAACAGTAGGAT+TGG 0.505029 4_4:+50325048 None:intergenic
AGTTCAATTGAAGGAATGGT+TGG 0.506067 4_4:+50324497 None:intergenic
TTGAGAAACAAAGAAGCCAT+TGG 0.506736 4_4:+50326620 None:intergenic
TGGTGGTAGCTACAATGATT+GGG 0.510224 4_4:-50326761 Msa0686010:exon
TCTTGGGTTCACAGATTCAT+CGG 0.511020 4_4:+50325030 None:intergenic
TCACGGTACAGATGATCAAA+AGG 0.512153 4_4:+50324668 None:intergenic
AGGCTTCGGCTTGCAGAGCA+AGG 0.512498 4_4:-50325154 Msa0686010:CDS
GGCTTTCCAAGGAGGTCGCA+TGG 0.512608 4_4:-50324454 Msa0686010:CDS
CTTGGGTTCACAGATTCATC+GGG 0.514137 4_4:+50325031 None:intergenic
TAACCTCAGTAAATTGAAAC+CGG 0.514324 4_4:-50324294 Msa0686010:CDS
GAGGGAACCATGCTGTCTCC+GGG 0.515651 4_4:-50324775 Msa0686010:CDS
CGGTCCAAAAGTTCAATTGA+AGG 0.520208 4_4:+50324488 None:intergenic
CTCGAGCATCTCCTTTACAT+TGG 0.523884 4_4:+50324946 None:intergenic
GCATGAAGCCCTTCATAACC+AGG 0.525971 4_4:+50324389 None:intergenic
GTGGTGGTAGCTACAATGAT+TGG 0.527281 4_4:-50326762 Msa0686010:exon
CTTGACAGTACCAAAAGGTC+CGG 0.528458 4_4:+50325081 None:intergenic
ATTCTTGCAGTCTCAAAATG+AGG 0.531707 4_4:+50324033 None:intergenic
CAATCTGAGCCATCAGGGAA+AGG 0.542774 4_4:+50324179 None:intergenic
TGTCTTCGACGAACCTGCGA+TGG 0.554872 4_4:-50324585 Msa0686010:CDS
TGAATCTGTGAACCCAAGAC+TGG 0.555469 4_4:-50325026 Msa0686010:CDS
GCTCTCAAACCTTTCCCTGA+TGG 0.559523 4_4:-50324188 Msa0686010:CDS
TGTCGCAACACGGCTTTCCA+AGG 0.563994 4_4:-50324465 Msa0686010:CDS
GTCTTCGACGAACCTGCGAT+GGG 0.565135 4_4:-50324584 Msa0686010:CDS
TATCGAGCCCAACCCATCGC+AGG 0.565521 4_4:+50324572 None:intergenic
GCTTTCCAAGGAGGTCGCAT+GGG 0.571535 4_4:-50324453 Msa0686010:CDS
TTTCATTAGTGATAATGCAT+AGG 0.571913 4_4:-50324108 Msa0686010:three_prime_UTR
ACAGAGAAAATAAACAAAGA+GGG 0.574776 4_4:+50324001 None:intergenic
TAGAATACGAAATTTAAGCC+CGG 0.575223 4_4:+50324757 None:intergenic
AATCCCTTGACAGTACCAAA+AGG 0.575926 4_4:+50325076 None:intergenic
CACAGAGAAAATAAACAAAG+AGG 0.576459 4_4:+50324000 None:intergenic
GTTCCTCGTTGAAAACGGCT+TGG 0.577634 4_4:+50324429 None:intergenic
TAACATGTTGACTCTATCTG+AGG 0.578577 4_4:+50325246 None:intergenic
TAGTGATAATGCATAGGTGT+AGG 0.579128 4_4:-50324102 Msa0686010:three_prime_UTR
GATTCATCGGGAACAACAGT+AGG 0.588765 4_4:+50325043 None:intergenic
TGGAAAGCCGTGTTGCGACA+CGG 0.596370 4_4:+50324468 None:intergenic
TTCACCAATATCAAGAGAGT+TGG 0.596908 4_4:-50324917 Msa0686010:CDS
TCATTCGCGGAAGCTTATCA+GGG 0.598350 4_4:+50324249 None:intergenic
CCTTCATAACCAGGGTGTGA+CGG 0.602424 4_4:+50324398 None:intergenic
TAGTGTCAATACCATCATCT+GGG 0.602710 4_4:+50324810 None:intergenic
ATTGTAAACCCATATCCTCA+TGG 0.612377 4_4:+50324541 None:intergenic
CGTCACACCCTGGTTATGAA+GGG 0.614212 4_4:-50324397 Msa0686010:CDS
ATTTAAGCCCGGAGACAGCA+TGG 0.617136 4_4:+50324768 None:intergenic
TTCTTGCAGTCTCAAAATGA+GGG 0.619293 4_4:+50324034 None:intergenic
CCTCAAACACTGAATTGAGA+TGG 0.625860 4_4:+50326784 None:intergenic
GATGTTGAGTCCATGAGCGA+TGG 0.626600 4_4:-50324641 Msa0686010:CDS
TGTTGTTGGATCCAAAACCC+AGG 0.631167 4_4:-50326571 Msa0686010:intron
GACACTATTGGAAAACAAGG+AGG 0.637402 4_4:-50324794 Msa0686010:CDS
GGCTTCGGCTTGCAGAGCAA+GGG 0.638726 4_4:-50325153 Msa0686010:CDS
GTTACCAATCTGAGCCATCA+GGG 0.641494 4_4:+50324174 None:intergenic
ATTGGGCGCAGAGAAAGAGA+AGG 0.643934 4_4:-50326744 Msa0686010:CDS
ATTGACACTATTGGAAAACA+AGG 0.644166 4_4:-50324797 Msa0686010:CDS
ACACTATTGGAAAACAAGGA+GGG 0.646374 4_4:-50324793 Msa0686010:CDS
TGCAGAGCAAGGGAAAGACC+AGG 0.647246 4_4:-50325143 Msa0686010:CDS
AGGTGAGGGAGTTGACCGAA+AGG 0.647633 4_4:-50325174 Msa0686010:CDS
TCTCAATTCAGTGTTTGAGG+TGG 0.654279 4_4:-50326781 Msa0686010:five_prime_UTR
TGGACTCAACATCTGAATCA+CGG 0.654697 4_4:+50324651 None:intergenic
ACAACTAAGACACAGAAACA+AGG 0.657054 4_4:+50324152 None:intergenic
CGCAACACGGCTTTCCAAGG+AGG 0.663318 4_4:-50324462 Msa0686010:CDS
TTACCGGTTTCAATTTACTG+AGG 0.679795 4_4:+50324291 None:intergenic
CTCGATACGCTCATACCATG+AGG 0.687908 4_4:-50324556 Msa0686010:CDS
CATGAAGCCCTTCATAACCA+GGG 0.689336 4_4:+50324390 None:intergenic
TTCGCGGAAGCTTATCAGGG+TGG 0.692799 4_4:+50324252 None:intergenic
CAATGTAAAGGAGATGCTCG+AGG 0.695514 4_4:-50324945 Msa0686010:CDS
AAGAACTCCAATCACAACAG+CGG 0.710718 4_4:+50326671 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTTGATATCAAGAATATT+TGG - chr4_4:50323902-50323921 Msa0686010:three_prime_UTR 15.0%
!! AAAAAATGAAAACAAATAAC+AGG + chr4_4:50325081-50325100 None:intergenic 15.0%
!!! CTATTTTCTCAAATTATTAT+TGG - chr4_4:50325233-50325252 Msa0686010:CDS 15.0%
!!! TTGATGCTTTTAATAATTGT+GGG - chr4_4:50324361-50324380 Msa0686010:CDS 20.0%
!!! TTTCAGAATTTTAGAATTGT+GGG - chr4_4:50324457-50324476 Msa0686010:CDS 20.0%
!! ATTTGTTTATATTGGAGATT+TGG - chr4_4:50324533-50324552 Msa0686010:CDS 20.0%
!! TTTGTTTATATTGGAGATTT+GGG - chr4_4:50324534-50324553 Msa0686010:CDS 20.0%
!! TTCATAGTTTATTGATAAGT+GGG - chr4_4:50324899-50324918 Msa0686010:CDS 20.0%
!!! TTTTCTCAAATTATTATTGG+TGG - chr4_4:50325236-50325255 Msa0686010:CDS 20.0%
!! TTGTTGAATATTATGTCAAT+GGG - chr4_4:50326532-50326551 Msa0686010:intron 20.0%
! CTTTGATGAAAATAAAACCT+GGG + chr4_4:50324201-50324220 None:intergenic 25.0%
! TCTTTGATGAAAATAAAACC+TGG + chr4_4:50324202-50324221 None:intergenic 25.0%
!!! CACAATTATTAAAAGCATCA+AGG + chr4_4:50324362-50324381 None:intergenic 25.0%
!! CTTGATGCTTTTAATAATTG+TGG - chr4_4:50324360-50324379 Msa0686010:CDS 25.0%
!! TTAATAATTGTGGGTTATTG+TGG - chr4_4:50324370-50324389 Msa0686010:CDS 25.0%
!!! GTTTCAGAATTTTAGAATTG+TGG - chr4_4:50324456-50324475 Msa0686010:CDS 25.0%
! TTTGAATCTATAGTTCAATG+TGG - chr4_4:50324497-50324516 Msa0686010:CDS 25.0%
! ATAAACAAATGTAGGCAAAA+TGG + chr4_4:50324523-50324542 None:intergenic 25.0%
! TTGCCTACATTTGTTTATAT+TGG - chr4_4:50324525-50324544 Msa0686010:CDS 25.0%
! TCTCCAATATAAACAAATGT+AGG + chr4_4:50324531-50324550 None:intergenic 25.0%
! CATAAAAAGAGAGAAGAATA+AGG + chr4_4:50324701-50324720 None:intergenic 25.0%
!! CTTATTCTTCTCTCTTTTTA+TGG - chr4_4:50324699-50324718 Msa0686010:CDS 25.0%
! GTTCATAGTTTATTGATAAG+TGG - chr4_4:50324898-50324917 Msa0686010:CDS 25.0%
!!! ATACCTGTTTTCTTTTGAAA+TGG - chr4_4:50325177-50325196 Msa0686010:CDS 25.0%
!!! TACCTGTTTTCTTTTGAAAT+GGG - chr4_4:50325178-50325197 Msa0686010:CDS 25.0%
!!! AACATTTGTTTTTGTTTCAC+TGG - chr4_4:50325381-50325400 Msa0686010:intron 25.0%
!!! TATCATGATTTTTTTTTGCC+AGG - chr4_4:50325462-50325481 Msa0686010:intron 25.0%
! GTTGTTGAATATTATGTCAA+TGG - chr4_4:50326531-50326550 Msa0686010:intron 25.0%
!! TTTCATTAGTGATAATGCAT+AGG - chr4_4:50326644-50326663 Msa0686010:CDS 25.0%
! AAAATAAACAAAGAGGGAAA+GGG + chr4_4:50326748-50326767 None:intergenic 25.0%
! ACAGAGAAAATAAACAAAGA+GGG + chr4_4:50326754-50326773 None:intergenic 25.0%
!!! TTATTTTCTCTGTGCTTTTT+TGG - chr4_4:50326761-50326780 Msa0686010:exon 25.0%
!!! TTAGCTTTCTTTTTTCCCAA+TGG - chr4_4:50324116-50324135 Msa0686010:three_prime_UTR 30.0%
! TGAAAATAAAACCTGGGTTT+TGG + chr4_4:50324195-50324214 None:intergenic 30.0%
! AACCATTTTGTGACAACTTT+TGG - chr4_4:50324670-50324689 Msa0686010:CDS 30.0%
! ACCATTTTGTGACAACTTTT+GGG - chr4_4:50324671-50324690 Msa0686010:CDS 30.0%
GATATTTGTACAACCATTCT+GGG + chr4_4:50324818-50324837 None:intergenic 30.0%
TGATATTTGTACAACCATTC+TGG + chr4_4:50324819-50324838 None:intergenic 30.0%
TAGTTTATTGATAAGTGGGT+AGG - chr4_4:50324903-50324922 Msa0686010:CDS 30.0%
AGCTTAGGGTTTAAATACTA+AGG + chr4_4:50324976-50324995 None:intergenic 30.0%
CTTTCTATCATTCAAGCTTA+GGG + chr4_4:50324990-50325009 None:intergenic 30.0%
!!! ACCTGTTTTCTTTTGAAATG+GGG - chr4_4:50325179-50325198 Msa0686010:CDS 30.0%
!!! GTTTTTGTTTCACTGGTTTT+TGG - chr4_4:50325388-50325407 Msa0686010:intron 30.0%
! TGTCTGTTATTCATCTTTTC+TGG + chr4_4:50325435-50325454 None:intergenic 30.0%
CATGTTAAAATCAGAGTTTG+TGG - chr4_4:50325522-50325541 Msa0686010:intron 30.0%
CAAAAACAGTTTCTTGCATT+AGG - chr4_4:50325634-50325653 Msa0686010:intron 30.0%
TTAGGTAAACAAGAAAAAGC+CGG - chr4_4:50325652-50325671 Msa0686010:intron 30.0%
ACTTGCAAATTCCAATGTAA+AGG - chr4_4:50325795-50325814 Msa0686010:intron 30.0%
TATACCAACTCTCTTGATAT+TGG + chr4_4:50325842-50325861 None:intergenic 30.0%
ATTGACACTATTGGAAAACA+AGG - chr4_4:50325955-50325974 Msa0686010:intron 30.0%
TAGAATACGAAATTTAAGCC+CGG + chr4_4:50325998-50326017 None:intergenic 30.0%
! TCTAAGAGAATTTTTGCAGT+TGG - chr4_4:50326014-50326033 Msa0686010:intron 30.0%
! CTAAGAGAATTTTTGCAGTT+GGG - chr4_4:50326015-50326034 Msa0686010:intron 30.0%
CATAATAACATGACAGCTTA+CGG - chr4_4:50326135-50326154 Msa0686010:intron 30.0%
CAAAAGTTCAATTGAAGGAA+TGG + chr4_4:50326262-50326281 None:intergenic 30.0%
! CATTCCTTCAATTGAACTTT+TGG - chr4_4:50326260-50326279 Msa0686010:intron 30.0%
TAACCTCAGTAAATTGAAAC+CGG - chr4_4:50326458-50326477 Msa0686010:intron 30.0%
TAATTCACGTGAACAATTAC+CGG + chr4_4:50326480-50326499 None:intergenic 30.0%
AAATAAACAAAGAGGGAAAG+GGG + chr4_4:50326747-50326766 None:intergenic 30.0%
GAAAATAAACAAAGAGGGAA+AGG + chr4_4:50326749-50326768 None:intergenic 30.0%
CACAGAGAAAATAAACAAAG+AGG + chr4_4:50326755-50326774 None:intergenic 30.0%
TGAGGATTGTTTCGCATTAA+GGG + chr4_4:50324035-50324054 None:intergenic 35.0%
ATGAGGATTGTTTCGCATTA+AGG + chr4_4:50324036-50324055 None:intergenic 35.0%
GTTGTGATTGGAGTTCTTAT+TGG - chr4_4:50324086-50324105 Msa0686010:three_prime_UTR 35.0%
TGAGAAACAAAGAAGCCATT+GGG + chr4_4:50324134-50324153 None:intergenic 35.0%
TTGAGAAACAAAGAAGCCAT+TGG + chr4_4:50324135-50324154 None:intergenic 35.0%
TCACAAATGCTCAAGTTAAG+AGG - chr4_4:50324587-50324606 Msa0686010:CDS 35.0%
GCTCAAGTTAAGAGGTTTAT+AGG - chr4_4:50324595-50324614 Msa0686010:CDS 35.0%
TCCCAAAAGTTGTCACAAAA+TGG + chr4_4:50324675-50324694 None:intergenic 35.0%
ACTTGTGGTTACATGTTGTT+GGG - chr4_4:50324871-50324890 Msa0686010:CDS 35.0%
CCTTTCTATCATTCAAGCTT+AGG + chr4_4:50324991-50325010 None:intergenic 35.0%
CCTAAGCTTGAATGATAGAA+AGG - chr4_4:50324988-50325007 Msa0686010:CDS 35.0%
TATGCAATCAAGAGACATAG+TGG - chr4_4:50325023-50325042 Msa0686010:CDS 35.0%
AGAGATTTGATCATCGGATT+TGG + chr4_4:50325058-50325077 None:intergenic 35.0%
AACAGGAGAGATTTGATCAT+CGG + chr4_4:50325064-50325083 None:intergenic 35.0%
ACCCCATTTCAAAAGAAAAC+AGG + chr4_4:50325183-50325202 None:intergenic 35.0%
!! CAAATTATTATTGGTGGCGT+TGG - chr4_4:50325242-50325261 Msa0686010:CDS 35.0%
TTGAGCACTAACACAAACAT+TGG + chr4_4:50325295-50325314 None:intergenic 35.0%
AGTGCTCAATAGTTTATGAG+CGG - chr4_4:50325306-50325325 Msa0686010:intron 35.0%
ATGTTTGCCTATACAAGTCA+AGG + chr4_4:50325366-50325385 None:intergenic 35.0%
!!! GGTTTTTGGTTTACAAGATG+AGG - chr4_4:50325402-50325421 Msa0686010:intron 35.0%
!! TAACATGTTGACTCTATCTG+AGG + chr4_4:50325509-50325528 None:intergenic 35.0%
AAATGCTGAGCTGAAAAAAC+AGG - chr4_4:50325558-50325577 Msa0686010:intron 35.0%
TTCACCAATATCAAGAGAGT+TGG - chr4_4:50325835-50325854 Msa0686010:intron 35.0%
! CTGGGTCTCTTTTGTAAAAT+GGG + chr4_4:50325927-50325946 None:intergenic 35.0%
! TCTGGGTCTCTTTTGTAAAA+TGG + chr4_4:50325928-50325947 None:intergenic 35.0%
TAGTGTCAATACCATCATCT+GGG + chr4_4:50325945-50325964 None:intergenic 35.0%
ATAGTGTCAATACCATCATC+TGG + chr4_4:50325946-50325965 None:intergenic 35.0%
!! GATGATGGTATTGACACTAT+TGG - chr4_4:50325946-50325965 Msa0686010:intron 35.0%
ACACTATTGGAAAACAAGGA+GGG - chr4_4:50325959-50325978 Msa0686010:intron 35.0%
ATACAATGTCAACGTCTGAT+AGG + chr4_4:50326053-50326072 None:intergenic 35.0%
CGTAAGCTGTCATGTTATTA+TGG + chr4_4:50326137-50326156 None:intergenic 35.0%
ATTGTAAACCCATATCCTCA+TGG + chr4_4:50326214-50326233 None:intergenic 35.0%
AGGATATGGGTTTACAATTC+CGG - chr4_4:50326216-50326235 Msa0686010:intron 35.0%
TGGTCTGATATAGAAGAAAC+CGG + chr4_4:50326238-50326257 None:intergenic 35.0%
AGTTCAATTGAAGGAATGGT+TGG + chr4_4:50326258-50326277 None:intergenic 35.0%
AAAGAGTTCCTCGTTGAAAA+CGG + chr4_4:50326331-50326350 None:intergenic 35.0%
!! AAGTTCTTTTCAAGACAGTG+AGG - chr4_4:50326427-50326446 Msa0686010:intron 35.0%
!! TTACCGGTTTCAATTTACTG+AGG + chr4_4:50326464-50326483 None:intergenic 35.0%
ACAACTAAGACACAGAAACA+AGG + chr4_4:50326603-50326622 None:intergenic 35.0%
! TAGTGATAATGCATAGGTGT+AGG - chr4_4:50326650-50326669 Msa0686010:CDS 35.0%
TTCTTGCAGTCTCAAAATGA+GGG + chr4_4:50326721-50326740 None:intergenic 35.0%
ATTCTTGCAGTCTCAAAATG+AGG + chr4_4:50326722-50326741 None:intergenic 35.0%
! AGATGGTAGTGTTGTTCTTC+TGG + chr4_4:50323954-50323973 None:intergenic 40.0%
CCTCAAACACTGAATTGAGA+TGG + chr4_4:50323971-50323990 None:intergenic 40.0%
CCATCTCAATTCAGTGTTTG+AGG - chr4_4:50323968-50323987 Msa0686010:three_prime_UTR 40.0%
TCTCAATTCAGTGTTTGAGG+TGG - chr4_4:50323971-50323990 Msa0686010:three_prime_UTR 40.0%
TGGTGGTAGCTACAATGATT+GGG - chr4_4:50323991-50324010 Msa0686010:three_prime_UTR 40.0%
!! CTTGATTTGCGTAGAGAATG+AGG + chr4_4:50324053-50324072 None:intergenic 40.0%
CTCATTCTCTACGCAAATCA+AGG - chr4_4:50324051-50324070 Msa0686010:three_prime_UTR 40.0%
ATTGTTACCGCTGTTGTGAT+TGG - chr4_4:50324074-50324093 Msa0686010:three_prime_UTR 40.0%
AAGAACTCCAATCACAACAG+CGG + chr4_4:50324084-50324103 None:intergenic 40.0%
! AGCTCTTGCATGATTTTCGT+TGG - chr4_4:50324260-50324279 Msa0686010:CDS 40.0%
!! GAGATTTGGGTTGTTAGATG+TGG - chr4_4:50324547-50324566 Msa0686010:CDS 40.0%
!!! AACCATTCTGGGACAACTTT+TGG + chr4_4:50324807-50324826 None:intergenic 40.0%
TCTCAAAAGTTTGCGAGTGT+CGG - chr4_4:50324841-50324860 Msa0686010:CDS 40.0%
GACTTGTGGTTACATGTTGT+TGG - chr4_4:50324870-50324889 Msa0686010:CDS 40.0%
TGGTTACATGTTGTTGGGTA+TGG - chr4_4:50324876-50324895 Msa0686010:CDS 40.0%
!!! GCTTTTGCCTTGACTTGTAT+AGG - chr4_4:50325356-50325375 Msa0686010:intron 40.0%
TGAGCTGAAAAAACAGGTGA+GGG - chr4_4:50325564-50325583 Msa0686010:intron 40.0%
!!! CAAGAAACTGTTTTTGAGCC+TGG + chr4_4:50325630-50325649 None:intergenic 40.0%
AATCCCTTGACAGTACCAAA+AGG + chr4_4:50325679-50325698 None:intergenic 40.0%
TCTTGGGTTCACAGATTCAT+CGG + chr4_4:50325725-50325744 None:intergenic 40.0%
CCAATATCTTTGCCAGTCTT+GGG + chr4_4:50325741-50325760 None:intergenic 40.0%
TCCAATATCTTTGCCAGTCT+TGG + chr4_4:50325742-50325761 None:intergenic 40.0%
TACAAAAGAGACCCAGATGA+TGG - chr4_4:50325931-50325950 Msa0686010:intron 40.0%
GACACTATTGGAAAACAAGG+AGG - chr4_4:50325958-50325977 Msa0686010:intron 40.0%
TCACGGTACAGATGATCAAA+AGG + chr4_4:50326087-50326106 None:intergenic 40.0%
TGGACTCAACATCTGAATCA+CGG + chr4_4:50326104-50326123 None:intergenic 40.0%
CGGTCCAAAAGTTCAATTGA+AGG + chr4_4:50326267-50326286 None:intergenic 40.0%
TCATGCTGCTAGAAGAACTA+TGG - chr4_4:50326380-50326399 Msa0686010:intron 40.0%
TCAACAACTGCTTTCATTCG+CGG + chr4_4:50326519-50326538 None:intergenic 40.0%
!! TTTTGGTACTTGCTCTAAGC+TGG - chr4_4:50326778-50326797 Msa0686010:five_prime_UTR 40.0%
! CAATTCAGTGTTTGAGGTGG+TGG - chr4_4:50323974-50323993 Msa0686010:three_prime_UTR 45.0%
GTGGTGGTAGCTACAATGAT+TGG - chr4_4:50323990-50324009 Msa0686010:three_prime_UTR 45.0%
AACATGGATGCGACGATTTG+AGG + chr4_4:50324165-50324184 None:intergenic 45.0%
AATCGTCGCATCCATGTTGT+TGG - chr4_4:50324167-50324186 Msa0686010:exon 45.0%
!!! GGGTTTTGGATCCAACAACA+TGG + chr4_4:50324181-50324200 None:intergenic 45.0%
TGTTGTTGGATCCAAAACCC+AGG - chr4_4:50324181-50324200 Msa0686010:CDS 45.0%
GTCCAAAAGTTGTCCCAGAA+TGG - chr4_4:50324802-50324821 Msa0686010:CDS 45.0%
! AGTGTCGGATACTTGACTTG+TGG - chr4_4:50324856-50324875 Msa0686010:CDS 45.0%
AAGAGACATAGTGGCGCAAT+TGG - chr4_4:50325032-50325051 Msa0686010:CDS 45.0%
! AAGCCTTATCCCACTAAGTG+GGG + chr4_4:50325129-50325148 None:intergenic 45.0%
! CAAGCCTTATCCCACTAAGT+GGG + chr4_4:50325130-50325149 None:intergenic 45.0%
!! TTGGTGCTACCTGTGAAAGT+CGG - chr4_4:50325261-50325280 Msa0686010:CDS 45.0%
CTGAGCTGAAAAAACAGGTG+AGG - chr4_4:50325563-50325582 Msa0686010:intron 45.0%
! CAAGAAAAAGCCGGACCTTT+TGG - chr4_4:50325661-50325680 Msa0686010:intron 45.0%
CTTGACAGTACCAAAAGGTC+CGG + chr4_4:50325674-50325693 None:intergenic 45.0%
! GGACCTTTTGGTACTGTCAA+GGG - chr4_4:50325673-50325692 Msa0686010:intron 45.0%
ATCGGGAACAACAGTAGGAT+TGG + chr4_4:50325707-50325726 None:intergenic 45.0%
GATTCATCGGGAACAACAGT+AGG + chr4_4:50325712-50325731 None:intergenic 45.0%
CTTGGGTTCACAGATTCATC+GGG + chr4_4:50325724-50325743 None:intergenic 45.0%
TGAATCTGTGAACCCAAGAC+TGG - chr4_4:50325726-50325745 Msa0686010:intron 45.0%
CCCAAGACTGGCAAAGATAT+TGG - chr4_4:50325738-50325757 Msa0686010:intron 45.0%
CTCGAGCATCTCCTTTACAT+TGG + chr4_4:50325809-50325828 None:intergenic 45.0%
CAATGTAAAGGAGATGCTCG+AGG - chr4_4:50325807-50325826 Msa0686010:intron 45.0%
GTTATTATGGCCATCGCTCA+TGG + chr4_4:50326124-50326143 None:intergenic 45.0%
ACGCTCATACCATGAGGATA+TGG - chr4_4:50326202-50326221 Msa0686010:intron 45.0%
CGCTCATACCATGAGGATAT+GGG - chr4_4:50326203-50326222 Msa0686010:intron 45.0%
CATGAAGCCCTTCATAACCA+GGG + chr4_4:50326365-50326384 None:intergenic 45.0%
TCATTCGCGGAAGCTTATCA+GGG + chr4_4:50326506-50326525 None:intergenic 45.0%
TTCATTCGCGGAAGCTTATC+AGG + chr4_4:50326507-50326526 None:intergenic 45.0%
GTTACCAATCTGAGCCATCA+GGG + chr4_4:50326581-50326600 None:intergenic 45.0%
ATTGGGCGCAGAGAAAGAGA+AGG - chr4_4:50324008-50324027 Msa0686010:three_prime_UTR 50.0%
TTTGAACCGACCCCACTTAG+TGG - chr4_4:50325116-50325135 Msa0686010:CDS 50.0%
TTGAACCGACCCCACTTAGT+GGG - chr4_4:50325117-50325136 Msa0686010:CDS 50.0%
CTTATCCCACTAAGTGGGGT+CGG + chr4_4:50325125-50325144 None:intergenic 50.0%
! CCAAGCCTTATCCCACTAAG+TGG + chr4_4:50325131-50325150 None:intergenic 50.0%
CCACTTAGTGGGATAAGGCT+TGG - chr4_4:50325128-50325147 Msa0686010:CDS 50.0%
!! GACACAGTACCGACTTTCAC+AGG + chr4_4:50325273-50325292 None:intergenic 50.0%
CACATTCAGCTGAGTTTGCC+TGG + chr4_4:50325483-50325502 None:intergenic 50.0%
! CGGACCTTTTGGTACTGTCA+AGG - chr4_4:50325672-50325691 Msa0686010:intron 50.0%
TAAAGGAGATGCTCGAGGTC+TGG - chr4_4:50325812-50325831 Msa0686010:intron 50.0%
ATTTAAGCCCGGAGACAGCA+TGG + chr4_4:50325987-50326006 None:intergenic 50.0%
GATGTTGAGTCCATGAGCGA+TGG - chr4_4:50326111-50326130 Msa0686010:intron 50.0%
CTCGATACGCTCATACCATG+AGG - chr4_4:50326196-50326215 Msa0686010:intron 50.0%
! TTTTGGACCGTGTCGCAACA+CGG - chr4_4:50326277-50326296 Msa0686010:intron 50.0%
GTTCCTCGTTGAAAACGGCT+TGG + chr4_4:50326326-50326345 None:intergenic 50.0%
! CTCTTTTATCCGTCACACCC+TGG - chr4_4:50326345-50326364 Msa0686010:intron 50.0%
CCTTCATAACCAGGGTGTGA+CGG + chr4_4:50326357-50326376 None:intergenic 50.0%
CGTCACACCCTGGTTATGAA+GGG - chr4_4:50326355-50326374 Msa0686010:intron 50.0%
GCATGAAGCCCTTCATAACC+AGG + chr4_4:50326366-50326385 None:intergenic 50.0%
GCTCTCAAACCTTTCCCTGA+TGG - chr4_4:50326564-50326583 Msa0686010:intron 50.0%
CAATCTGAGCCATCAGGGAA+AGG + chr4_4:50326576-50326595 None:intergenic 50.0%
CTTTCCCTGATGGCTCAGAT+TGG - chr4_4:50326574-50326593 Msa0686010:CDS 50.0%
GGTTACCAATCTGAGCCATC+AGG + chr4_4:50326582-50326601 None:intergenic 50.0%
AGCGGGTGGGTATGGTTCAT+AGG - chr4_4:50324933-50324952 Msa0686010:CDS 55.0%
CGACCCCACTTAGTGGGATA+AGG - chr4_4:50325123-50325142 Msa0686010:CDS 55.0%
AGGTGAGGGAGTTGACCGAA+AGG - chr4_4:50325578-50325597 Msa0686010:intron 55.0%
GGGAGTTGACCGAAAGGCTT+CGG - chr4_4:50325584-50325603 Msa0686010:intron 55.0%
CTCTGCAAGCCGAAGCCTTT+CGG + chr4_4:50325596-50325615 None:intergenic 55.0%
TGCAGAGCAAGGGAAAGACC+AGG - chr4_4:50325609-50325628 Msa0686010:intron 55.0%
TGTCTTCGACGAACCTGCGA+TGG - chr4_4:50326167-50326186 Msa0686010:intron 55.0%
GTCTTCGACGAACCTGCGAT+GGG - chr4_4:50326168-50326187 Msa0686010:intron 55.0%
TGGAAAGCCGTGTTGCGACA+CGG + chr4_4:50326287-50326306 None:intergenic 55.0%
TGTCGCAACACGGCTTTCCA+AGG - chr4_4:50326287-50326306 Msa0686010:intron 55.0%
GCTTTCCAAGGAGGTCGCAT+GGG - chr4_4:50326299-50326318 Msa0686010:intron 55.0%
!! GGACCAAGCCGTTTTCAACG+AGG - chr4_4:50326320-50326339 Msa0686010:intron 55.0%
CCGTCACACCCTGGTTATGA+AGG - chr4_4:50326354-50326373 Msa0686010:intron 55.0%
TTCGCGGAAGCTTATCAGGG+TGG + chr4_4:50326503-50326522 None:intergenic 55.0%
AAGTGGGTAGGTCGCTCGAG+CGG - chr4_4:50324915-50324934 Msa0686010:CDS 60.0%
! AGGCTTCGGCTTGCAGAGCA+AGG - chr4_4:50325598-50325617 Msa0686010:intron 60.0%
! GGCTTCGGCTTGCAGAGCAA+GGG - chr4_4:50325599-50325618 Msa0686010:intron 60.0%
GGAGGGAACCATGCTGTCTC+CGG - chr4_4:50325976-50325995 Msa0686010:intron 60.0%
GAGGGAACCATGCTGTCTCC+GGG - chr4_4:50325977-50325996 Msa0686010:intron 60.0%
TCGACGAACCTGCGATGGGT+TGG - chr4_4:50326172-50326191 Msa0686010:intron 60.0%
! CGACGAACCTGCGATGGGTT+GGG - chr4_4:50326173-50326192 Msa0686010:intron 60.0%
TATCGAGCCCAACCCATCGC+AGG + chr4_4:50326183-50326202 None:intergenic 60.0%
CGCAACACGGCTTTCCAAGG+AGG - chr4_4:50326290-50326309 Msa0686010:intron 60.0%
GGCTTTCCAAGGAGGTCGCA+TGG - chr4_4:50326298-50326317 Msa0686010:intron 60.0%
TTGGTCCCATGCGACCTCCT+TGG + chr4_4:50326307-50326326 None:intergenic 60.0%
AGTGGGTAGGTCGCTCGAGC+GGG - chr4_4:50324916-50324935 Msa0686010:CDS 65.0%
GGTAGGTCGCTCGAGCGGGT+GGG - chr4_4:50324920-50324939 Msa0686010:CDS 70.0%
GTCGCTCGAGCGGGTGGGTA+TGG - chr4_4:50324925-50324944 Msa0686010:CDS 70.0%
GGGTAGGTCGCTCGAGCGGG+TGG - chr4_4:50324919-50324938 Msa0686010:CDS 75.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 50323899 50326875 50323899 ID=Msa0686010;Name=Msa0686010
chr4_4 mRNA 50323899 50326875 50323899 ID=Msa0686010-mRNA-1;Parent=Msa0686010;Name=Msa0686010-mRNA-1;_AED=0.01;_eAED=0.01;_QI=106|1|1|1|1|1|2|276|437
chr4_4 exon 50323899 50325290 50323899 ID=Msa0686010-mRNA-1:exon:12859;Parent=Msa0686010-mRNA-1
chr4_4 exon 50326572 50326875 50326572 ID=Msa0686010-mRNA-1:exon:12858;Parent=Msa0686010-mRNA-1
chr4_4 five_prime_UTR 50326770 50326875 50326770 ID=Msa0686010-mRNA-1:five_prime_utr;Parent=Msa0686010-mRNA-1
chr4_4 CDS 50326572 50326769 50326572 ID=Msa0686010-mRNA-1:cds;Parent=Msa0686010-mRNA-1
chr4_4 CDS 50324175 50325290 50324175 ID=Msa0686010-mRNA-1:cds;Parent=Msa0686010-mRNA-1
chr4_4 three_prime_UTR 50323899 50324174 50323899 ID=Msa0686010-mRNA-1:three_prime_utr;Parent=Msa0686010-mRNA-1
Gene Sequence

>Msa0686010

ATGATTGGGCGCAGAGAAAGAGAAGGACCCTTAATGCGAAACAATCCTCATTCTCTACGCAAATCAAGGATTGTTACCGCTGTTGTGATTGGAGTTCTTATTGGATGCATCTTAGCTTTCTTTTTTCCCAATGGCTTCTTTGTTTCTCAATCTATTACCTCAAATCGTCGCATCCATGTTGTTGGATCCAAAACCCAGGCAAACTCAGCTGAATGTGAATCCTCAGATAGAGTCAACATGTTAAAATCAGAGTTTGTGGCAGTATCAGATAAAAATGCTGAGCTGAAAAAACAGGTGAGGGAGTTGACCGAAAGGCTTCGGCTTGCAGAGCAAGGGAAAGACCAGGCTCAAAAACAGTTTCTTGCATTAGGTAAACAAGAAAAAGCCGGACCTTTTGGTACTGTCAAGGGATTAAGAACCAATCCTACTGTTGTTCCCGATGAATCTGTGAACCCAAGACTGGCAAAGATATTGGAGAAAAACGCAGTTAAAAGAGAGATCATAGTTGCACTTGCAAATTCCAATGTAAAGGAGATGCTCGAGGTCTGGTTCACCAATATCAAGAGAGTTGGTATAACTAATTGTCTAGTTGTTGCTTTAGACGATGAGATTTCGAAGTTTTGTGAATCGAATCAAGTCCCATTTTACAAAAGAGACCCAGATGATGGTATTGACACTATTGGAAAACAAGGAGGGAACCATGCTGTCTCCGGGCTTAAATTTCGTATTCTAAGAGAATTTTTGCAGTTGGGATATAGTGTTCTCCTATCAGACGTTGACATTGTATATTTGCAGAATCCTTTTGATCATCTGTACCGTGATTCAGATGTTGAGTCCATGAGCGATGGCCATAATAACATGACAGCTTACGGCTATAACGATGTCTTCGACGAACCTGCGATGGGTTGGGCTCGATACGCTCATACCATGAGGATATGGGTTTACAATTCCGGTTTCTTCTATATCAGACCAACCATTCCTTCAATTGAACTTTTGGACCGTGTCGCAACACGGCTTTCCAAGGAGGTCGCATGGGACCAAGCCGTTTTCAACGAGGAACTCTTTTATCCGTCACACCCTGGTTATGAAGGGCTTCATGCTGCTAGAAGAACTATGGATATGTATCTCTTTATGAACAGCAAAGTTCTTTTCAAGACAGTGAGGAACGATGCTAACCTCAGTAAATTGAAACCGGTAATTGTTCACGTGAATTACCACCCTGATAAGCTTCCGCGAATGAAAGCAGTTGTTGAATATTATGTCAATGGGAAACAAGATGCTCTCAAACCTTTCCCTGATGGCTCAGATTGGTAA
Protein sequence

>Msa0686010

MIGRREREGPLMRNNPHSLRKSRIVTAVVIGVLIGCILAFFFPNGFFVSQSITSNRRIHVVGSKTQANSAECESSDRVNMLKSEFVAVSDKNAELKKQVRELTERLRLAEQGKDQAQKQFLALGKQEKAGPFGTVKGLRTNPTVVPDESVNPRLAKILEKNAVKREIIVALANSNVKEMLEVWFTNIKRVGITNCLVVALDDEISKFCESNQVPFYKRDPDDGIDTIGKQGGNHAVSGLKFRILREFLQLGYSVLLSDVDIVYLQNPFDHLYRDSDVESMSDGHNNMTAYGYNDVFDEPAMGWARYAHTMRIWVYNSGFFYIRPTIPSIELLDRVATRLSKEVAWDQAVFNEELFYPSHPGYEGLHAARRTMDMYLFMNSKVLFKTVRNDANLSKLKPVIVHVNYHPDKLPRMKAVVEYYVNGKQDALKPFPDGSDW*