AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0605380

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0605380 MtrunA17_Chr4g0034611 97.941 437 9 0 1 437 1 437 0.0 891
Msa0605380 MtrunA17_Chr4g0034621 84.703 438 66 1 1 437 1 438 0.0 765
Msa0605380 MtrunA17_Chr4g0034641 80.505 436 79 2 1 435 1 431 0.0 726
Msa0605380 MtrunA17_Chr4g0034631 80.963 436 75 2 1 435 1 429 0.0 723
Msa0605380 MtrunA17_Chr4g0034651 79.310 435 82 1 1 435 34 460 0.0 700
Msa0605380 MtrunA17_Chr4g0034661 80.000 435 72 2 1 435 1 420 0.0 691
Msa0605380 MtrunA17_Chr1g0154701 73.409 440 101 3 1 436 1 428 0.0 676
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0605380 AT1G19360.1 72.769 437 109 3 1 436 1 428 0.0 658
Msa0605380 AT1G19360.2 72.769 437 109 3 1 436 1 428 0.0 658
Msa0605380 AT1G75110.1 71.918 438 111 3 1 436 1 428 0.0 652
Msa0605380 AT1G75120.1 61.327 437 132 5 1 435 1 402 0.0 540
Msa0605380 AT1G70630.5 28.169 213 122 6 148 354 231 418 1.73e-13 72.8
Msa0605380 AT1G70630.2 28.169 213 122 6 148 354 262 449 1.91e-13 72.8
Msa0605380 AT1G70630.1 28.169 213 122 6 148 354 262 449 1.91e-13 72.8
Msa0605380 AT1G70630.4 28.169 213 122 6 148 354 447 634 2.49e-13 72.4
Msa0605380 AT1G70630.3 28.169 213 122 6 148 354 478 665 2.60e-13 72.4
Msa0605380 AT1G70630.7 28.169 213 122 6 148 354 399 586 2.62e-13 72.4
Msa0605380 AT1G70630.6 28.169 213 122 6 148 354 468 655 2.62e-13 72.4

Find 116 sgRNAs with CRISPR-Local

Find 217 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTCCTTCAATTGAACTTT+TGG 0.197156 4_2:-51183458 Msa0605380:CDS
TTTATGAGCAATTTGAATTA+TGG 0.204726 4_2:-51183028 Msa0605380:three_prime_UTR
TGAAAATAAAACCTGGGTTT+TGG 0.224761 4_2:+51185545 None:intergenic
ATGAGGATTGTTTCGCATTA+AGG 0.231351 4_2:+51185704 None:intergenic
TCCAATATCTTTGCCAGTCT+TGG 0.235716 4_2:+51183979 None:intergenic
TTCATTCGCGGAAGCTTATC+AGG 0.249563 4_2:+51183214 None:intergenic
AGATGGTAGTGTTGTTCTTC+TGG 0.271544 4_2:+51185786 None:intergenic
TATACCAACTCTCTTGATAT+TGG 0.305862 4_2:+51183879 None:intergenic
CGTAAGCTGTCATGTTATTA+TGG 0.320845 4_2:+51183584 None:intergenic
AGGATATGGGTTTACAATTC+CGG 0.324078 4_2:-51183502 Msa0605380:CDS
GTTGTGATTGGAGTTCTTAT+TGG 0.330486 4_2:-51185651 Msa0605380:CDS
CTCTGCAAGCCGAAGCCTTT+CGG 0.335447 4_2:+51184125 None:intergenic
ACGCTCATACCATGAGGATA+TGG 0.335819 4_2:-51183516 Msa0605380:CDS
CCCAAGACTGGCAAAGATAT+TGG 0.338770 4_2:-51183980 Msa0605380:CDS
CCAATATCTTTGCCAGTCTT+GGG 0.362824 4_2:+51183980 None:intergenic
TAATTCACGTGAACAATTAC+CGG 0.366362 4_2:+51183241 None:intergenic
ACAACTAAGACATGGAAATA+AGG 0.372033 4_2:+51183118 None:intergenic
AAAATAAATAAAGAGGGAAA+AGG 0.386345 4_2:+51182973 None:intergenic
TCATGCTGCTAGAAGAACTA+TGG 0.394162 4_2:-51183338 Msa0605380:CDS
CGACGAACCTGCGATGGGTT+GGG 0.394380 4_2:-51183545 Msa0605380:CDS
TGAGGATTGTTTCGCATTAA+GGG 0.394783 4_2:+51185705 None:intergenic
CCATCTCAATTCAGTGTTTG+AGG 0.397352 4_2:-51185769 Msa0605380:five_prime_UTR
GATGATGGTATTGACACTAT+TGG 0.398863 4_2:-51183772 Msa0605380:CDS
GGAGGGAACCATGCTGTCTC+CGG 0.406427 4_2:-51183742 Msa0605380:CDS
GTTGTTGAATATTATGTCAA+TGG 0.415566 4_2:-51183187 Msa0605380:CDS
CAATTCAGTGTTTGAGGTGG+TGG 0.433911 4_2:-51185763 Msa0605380:five_prime_UTR
TCGACGAACCTGCGATGGGT+TGG 0.436451 4_2:-51183546 Msa0605380:CDS
GGGAGTTGACCGAAAGGCTT+CGG 0.438259 4_2:-51184134 Msa0605380:CDS
GGTTACCAATCTGAGCCATC+AGG 0.443252 4_2:+51183139 None:intergenic
ATAGTGTCAATACCATCATC+TGG 0.444142 4_2:+51183775 None:intergenic
ATACAATGTCAACGTCTGAT+AGG 0.444574 4_2:+51183668 None:intergenic
CCGTCACACCCTGGTTATGA+AGG 0.446141 4_2:-51183364 Msa0605380:CDS
ATTGTTACCGCTGTTGTGAT+TGG 0.448923 4_2:-51185663 Msa0605380:CDS
AATCGTCGCATCCATGTTGT+TGG 0.454605 4_2:-51185570 Msa0605380:CDS
GTTATTATGGCCATCGCTCA+TGG 0.461921 4_2:+51183597 None:intergenic
CATAATAACATGACAGCTTA+CGG 0.462428 4_2:-51183583 Msa0605380:CDS
AAATAAATAAAGAGGGAAAA+GGG 0.465933 4_2:+51182974 None:intergenic
CTCATTCTCTACGCAAATCA+AGG 0.468828 4_2:-51185686 Msa0605380:CDS
CAAAAGTTCAATTGAAGGAA+TGG 0.471345 4_2:+51183459 None:intergenic
CATGTTAAAATCAGAGTTTG+TGG 0.477132 4_2:-51184196 Msa0605380:CDS
TTGTTGAATATTATGTCAAT+GGG 0.480177 4_2:-51183186 Msa0605380:CDS
AAAGAGTTCCTCGTTGAAAA+CGG 0.480750 4_2:+51183390 None:intergenic
CTTTCCCTGATGGCTCAGAT+TGG 0.480932 4_2:-51183144 Msa0605380:CDS
CTTGATTTGCGTAGAGAATG+AGG 0.482124 4_2:+51185687 None:intergenic
TAAAGGAGATGCTCGAGGTC+TGG 0.483638 4_2:-51183906 Msa0605380:CDS
TCAACAACTGCTTTCATTCG+CGG 0.485251 4_2:+51183202 None:intergenic
TTAGCTTTCTTCTTTCCCAA+TGG 0.486248 4_2:-51185621 Msa0605380:CDS
TTGGTCCCATGCGACCTCCT+TGG 0.491130 4_2:+51183414 None:intergenic
CGCTCATACCATGAGGATAT+GGG 0.494439 4_2:-51183515 Msa0605380:CDS
TGGTCTGATATAGAAGAAAC+CGG 0.496586 4_2:+51183483 None:intergenic
TACAAAAGAGACCCAGATGA+TGG 0.498054 4_2:-51183787 Msa0605380:CDS
TTGAGAAACGAAGAAGCCAT+TGG 0.498194 4_2:+51185605 None:intergenic
TGAGAAACGAAGAAGCCATT+GGG 0.499912 4_2:+51185606 None:intergenic
ACTTGCAAATTCCAATGTAA+AGG 0.500585 4_2:-51183923 Msa0605380:CDS
ATCGGGAACAACAGTAGGAT+TGG 0.505029 4_2:+51184014 None:intergenic
TCACGGTATAGATGATCAAA+AGG 0.505121 4_2:+51183634 None:intergenic
AGTTCAATTGAAGGAATGGT+TGG 0.506067 4_2:+51183463 None:intergenic
TGGTGGTAGCTACAATGATT+GGG 0.510224 4_2:-51185746 Msa0605380:exon
TCTTGGGTTCACAGATTCAT+CGG 0.511020 4_2:+51183996 None:intergenic
AGGCTTCGGCTTGCAGAGCA+AGG 0.512498 4_2:-51184120 Msa0605380:CDS
CACAGAGAAAATAAATAAAG+AGG 0.512601 4_2:+51182966 None:intergenic
GGCTTTCCAAGGAGGTCGCA+TGG 0.512608 4_2:-51183420 Msa0605380:CDS
CTTGGGTTCACAGATTCATC+GGG 0.514137 4_2:+51183997 None:intergenic
TAACCTCAGTAAATTGAAAC+CGG 0.514324 4_2:-51183260 Msa0605380:CDS
CGGTCCAAAAGTTCAATTGA+AGG 0.515275 4_2:+51183454 None:intergenic
GAGGGAACCATGCTGTCTCC+GGG 0.515651 4_2:-51183741 Msa0605380:CDS
CTCGAGCATCTCCTTTACAT+TGG 0.523884 4_2:+51183912 None:intergenic
GCATGAAGCCCTTCATAACC+AGG 0.525971 4_2:+51183355 None:intergenic
GTGGTGGTAGCTACAATGAT+TGG 0.527281 4_2:-51185747 Msa0605380:exon
CTTGACAGTACCAAAAGGTC+CGG 0.528458 4_2:+51184047 None:intergenic
AAACTTGCAGTCTCAAAATG+AGG 0.531707 4_2:+51182999 None:intergenic
CAATCTGAGCCATCAGGGAA+AGG 0.542774 4_2:+51183145 None:intergenic
TGTCTTCGACGAACCTGCGA+TGG 0.554872 4_2:-51183551 Msa0605380:CDS
TGAATCTGTGAACCCAAGAC+TGG 0.555469 4_2:-51183992 Msa0605380:CDS
GTCTTCGACGAACCTGCGAT+GGG 0.565135 4_2:-51183550 Msa0605380:CDS
TATCGAGCCCAACCCATCGC+AGG 0.565521 4_2:+51183538 None:intergenic
GCTTTCCAAGGAGGTCGCAT+GGG 0.571535 4_2:-51183419 Msa0605380:CDS
TTTCATTAGTGATAATGCAT+AGG 0.571913 4_2:-51183074 Msa0605380:three_prime_UTR
TAGAATACGAAATTTAAGCC+CGG 0.575223 4_2:+51183723 None:intergenic
AATCCCTTGACAGTACCAAA+AGG 0.575926 4_2:+51184042 None:intergenic
GTTCCTCGTTGAAAACGGCT+TGG 0.577634 4_2:+51183395 None:intergenic
TAACATGTTGACTCTATCTG+AGG 0.578577 4_2:+51184212 None:intergenic
TAGTGATAATGCATAGGTGT+AGG 0.579128 4_2:-51183068 Msa0605380:three_prime_UTR
TGTAGCAACACGGCTTTCCA+AGG 0.581666 4_2:-51183431 Msa0605380:CDS
TGGAAAGCCGTGTTGCTACA+CGG 0.587855 4_2:+51183434 None:intergenic
GATTCATCGGGAACAACAGT+AGG 0.588765 4_2:+51184009 None:intergenic
ACAGAGAAAATAAATAAAGA+GGG 0.592227 4_2:+51182967 None:intergenic
GCTCTCAAACCTTTCCCTGA+TGG 0.593787 4_2:-51183154 Msa0605380:CDS
TTCACCAATATCAAGAGAGT+TGG 0.596908 4_2:-51183883 Msa0605380:CDS
TCATTCGCGGAAGCTTATCA+GGG 0.598350 4_2:+51183215 None:intergenic
CCTTCATAACCAGGGTGTGA+CGG 0.602424 4_2:+51183364 None:intergenic
TAGTGTCAATACCATCATCT+GGG 0.602710 4_2:+51183776 None:intergenic
ATTGTAAACCCATATCCTCA+TGG 0.612377 4_2:+51183507 None:intergenic
CGTCACACCCTGGTTATGAA+GGG 0.614212 4_2:-51183363 Msa0605380:CDS
GTTACCAATCTGAGCCATCA+GGG 0.614642 4_2:+51183140 None:intergenic
ATTTAAGCCCGGAGACAGCA+TGG 0.617136 4_2:+51183734 None:intergenic
AACTTGCAGTCTCAAAATGA+GGG 0.619293 4_2:+51183000 None:intergenic
CCTCAAACACTGAATTGAGA+TGG 0.625860 4_2:+51185769 None:intergenic
GATGTTGAGTCCATGAGCGA+TGG 0.626600 4_2:-51183607 Msa0605380:CDS
TGTTGTTGGATCCAAAACCC+AGG 0.631167 4_2:-51185556 Msa0605380:intron
GACACTATTGGAAAACAAGG+AGG 0.637402 4_2:-51183760 Msa0605380:CDS
GGCTTCGGCTTGCAGAGCAA+GGG 0.638726 4_2:-51184119 Msa0605380:CDS
ATTGGGCGCAGAGAAAGAGA+AGG 0.643934 4_2:-51185729 Msa0605380:CDS
ATTGACACTATTGGAAAACA+AGG 0.644166 4_2:-51183763 Msa0605380:CDS
TGGACTCAACATCTGAATCA+CGG 0.646362 4_2:+51183617 None:intergenic
ACACTATTGGAAAACAAGGA+GGG 0.646374 4_2:-51183759 Msa0605380:CDS
TGCAGAGCAAGGGAAAGACC+AGG 0.647246 4_2:-51184109 Msa0605380:CDS
AGGTGAGGGAGTTGACCGAA+AGG 0.647633 4_2:-51184140 Msa0605380:CDS
TCTCAATTCAGTGTTTGAGG+TGG 0.654279 4_2:-51185766 Msa0605380:five_prime_UTR
TTACCGGTTTCAATTTACTG+AGG 0.679795 4_2:+51183257 None:intergenic
CTCGATACGCTCATACCATG+AGG 0.687908 4_2:-51183522 Msa0605380:CDS
CATGAAGCCCTTCATAACCA+GGG 0.689336 4_2:+51183356 None:intergenic
TTCGCGGAAGCTTATCAGGG+TGG 0.692799 4_2:+51183218 None:intergenic
CAATGTAAAGGAGATGCTCG+AGG 0.695514 4_2:-51183911 Msa0605380:CDS
AGCAACACGGCTTTCCAAGG+AGG 0.700999 4_2:-51183428 Msa0605380:CDS
AAGAACTCCAATCACAACAG+CGG 0.710718 4_2:+51185656 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTCTTTCTTTTTTGAATTT+TGG - chr4_2:51183206-51183225 Msa0605380:CDS 15.0%
!!! TTCTTTCTTTTTTGAATTTT+GGG - chr4_2:51183207-51183226 Msa0605380:CDS 15.0%
!! AAAAAATGAAAACAAATAAC+AGG + chr4_2:51184068-51184087 None:intergenic 15.0%
!!! CTATTTTCTCAAATTATTAT+TGG - chr4_2:51184220-51184239 Msa0605380:CDS 15.0%
!!! TTGATGCTTTTAATAATTGT+GGG - chr4_2:51183348-51183367 Msa0605380:CDS 20.0%
!!! TTTCAGAATTTTAGAATTGT+GGG - chr4_2:51183444-51183463 Msa0605380:CDS 20.0%
!! ATTTGTTTATATTGGAGATT+TGG - chr4_2:51183520-51183539 Msa0605380:CDS 20.0%
!! TTTGTTTATATTGGAGATTT+GGG - chr4_2:51183521-51183540 Msa0605380:CDS 20.0%
!! TTCATAGTTTATTGATAAGT+GGG - chr4_2:51183886-51183905 Msa0605380:CDS 20.0%
!!! TTTTCTCAAATTATTATTGG+TGG - chr4_2:51184223-51184242 Msa0605380:CDS 20.0%
!! TTGTTGAATATTATGTCAAT+GGG - chr4_2:51185519-51185538 Msa0605380:intron 20.0%
!! TTTATGAGCAATTTGAATTA+TGG - chr4_2:51185677-51185696 Msa0605380:CDS 20.0%
!! AAATAAATAAAGAGGGAAAA+GGG + chr4_2:51185734-51185753 None:intergenic 20.0%
!! AAAATAAATAAAGAGGGAAA+AGG + chr4_2:51185735-51185754 None:intergenic 20.0%
!! ACAGAGAAAATAAATAAAGA+GGG + chr4_2:51185741-51185760 None:intergenic 20.0%
! CTTTAGTGAAAATAAAACCT+GGG + chr4_2:51183169-51183188 None:intergenic 25.0%
! TCTTTAGTGAAAATAAAACC+TGG + chr4_2:51183170-51183189 None:intergenic 25.0%
!!! CACAATTATTAAAAGCATCA+AGG + chr4_2:51183349-51183368 None:intergenic 25.0%
!! CTTGATGCTTTTAATAATTG+TGG - chr4_2:51183347-51183366 Msa0605380:CDS 25.0%
!!! GTTTCAGAATTTTAGAATTG+TGG - chr4_2:51183443-51183462 Msa0605380:CDS 25.0%
! TTTGAATCTATAGTTCAATG+TGG - chr4_2:51183484-51183503 Msa0605380:CDS 25.0%
! ATAAACAAATGTAGGCAAAA+TGG + chr4_2:51183510-51183529 None:intergenic 25.0%
! TTGCCTACATTTGTTTATAT+TGG - chr4_2:51183512-51183531 Msa0605380:CDS 25.0%
! TCTCCAATATAAACAAATGT+AGG + chr4_2:51183518-51183537 None:intergenic 25.0%
! CATAAAAAGAGAGAAGAATA+AGG + chr4_2:51183688-51183707 None:intergenic 25.0%
!! CTTATTCTTCTCTCTTTTTA+TGG - chr4_2:51183686-51183705 Msa0605380:CDS 25.0%
! GTTCATAGTTTATTGATAAG+TGG - chr4_2:51183885-51183904 Msa0605380:CDS 25.0%
!!! ATACCTGTTTTCTTTTGAAA+TGG - chr4_2:51184164-51184183 Msa0605380:CDS 25.0%
!!! TACCTGTTTTCTTTTGAAAT+GGG - chr4_2:51184165-51184184 Msa0605380:CDS 25.0%
!!! AACATTTGTTTTTGTTTCAC+TGG - chr4_2:51184368-51184387 Msa0605380:intron 25.0%
!!! TATCATGATTTTTTTTTGCC+AGG - chr4_2:51184449-51184468 Msa0605380:intron 25.0%
! GTTGTTGAATATTATGTCAA+TGG - chr4_2:51185518-51185537 Msa0605380:intron 25.0%
!! TTTCATTAGTGATAATGCAT+AGG - chr4_2:51185631-51185650 Msa0605380:CDS 25.0%
! CACAGAGAAAATAAATAAAG+AGG + chr4_2:51185742-51185761 None:intergenic 25.0%
!!! CTCTTTATTTATTTTCTCTG+TGG - chr4_2:51185740-51185759 Msa0605380:exon 25.0%
!! TTATTTTCTCTGTGGTTTTT+TGG - chr4_2:51185748-51185767 Msa0605380:exon 25.0%
! TGAAAATAAAACCTGGGTTT+TGG + chr4_2:51183163-51183182 None:intergenic 30.0%
TTAATAATTGTGGGTCATTG+TGG - chr4_2:51183357-51183376 Msa0605380:CDS 30.0%
TCACAAATGCTTAAGTTAAG+AGG - chr4_2:51183574-51183593 Msa0605380:CDS 30.0%
GCTTAAGTTAAGAGGTTTAT+AGG - chr4_2:51183582-51183601 Msa0605380:CDS 30.0%
! AACCATTTTGTGACAACTTT+TGG - chr4_2:51183657-51183676 Msa0605380:CDS 30.0%
! ACCATTTTGTGACAACTTTT+GGG - chr4_2:51183658-51183677 Msa0605380:CDS 30.0%
GATATTTGTACAACCATTCT+GGG + chr4_2:51183805-51183824 None:intergenic 30.0%
TGATATTTGTACAACCATTC+TGG + chr4_2:51183806-51183825 None:intergenic 30.0%
TAGTTTATTGATAAGTGGGT+AGG - chr4_2:51183890-51183909 Msa0605380:CDS 30.0%
AGCTTAGGGTTTAAATACTA+AGG + chr4_2:51183963-51183982 None:intergenic 30.0%
CTTTCTATCATTCAAGCTTA+GGG + chr4_2:51183977-51183996 None:intergenic 30.0%
TATGCAATCAAGAGACATAA+TGG - chr4_2:51184010-51184029 Msa0605380:CDS 30.0%
!!! ACCTGTTTTCTTTTGAAATG+GGG - chr4_2:51184166-51184185 Msa0605380:CDS 30.0%
ATGTTTGTCTATACAAGTCA+AGG + chr4_2:51184353-51184372 None:intergenic 30.0%
!!! GTTTTTGTTTCACTGGTTTT+TGG - chr4_2:51184375-51184394 Msa0605380:intron 30.0%
!!! TGGTTTTTGGTTTACAAGAT+GGG - chr4_2:51184388-51184407 Msa0605380:intron 30.0%
! TGTCTGTTATTCATCTTTTC+TGG + chr4_2:51184422-51184441 None:intergenic 30.0%
CATGTTAAAATCAGAGTTTG+TGG - chr4_2:51184509-51184528 Msa0605380:intron 30.0%
CAAAAACAGTTTCTTGCATT+AGG - chr4_2:51184621-51184640 Msa0605380:intron 30.0%
TTAGGTAAACAAGAAAAAGC+CGG - chr4_2:51184639-51184658 Msa0605380:intron 30.0%
ACTTGCAAATTCCAATGTAA+AGG - chr4_2:51184782-51184801 Msa0605380:intron 30.0%
TATACCAACTCTCTTGATAT+TGG + chr4_2:51184829-51184848 None:intergenic 30.0%
ATTGACACTATTGGAAAACA+AGG - chr4_2:51184942-51184961 Msa0605380:intron 30.0%
TAGAATACGAAATTTAAGCC+CGG + chr4_2:51184985-51185004 None:intergenic 30.0%
! TCTAAGAGAATTTTTGCAGT+TGG - chr4_2:51185001-51185020 Msa0605380:intron 30.0%
! CTAAGAGAATTTTTGCAGTT+GGG - chr4_2:51185002-51185021 Msa0605380:intron 30.0%
CATAATAACATGACAGCTTA+CGG - chr4_2:51185122-51185141 Msa0605380:intron 30.0%
CAAAAGTTCAATTGAAGGAA+TGG + chr4_2:51185249-51185268 None:intergenic 30.0%
! CATTCCTTCAATTGAACTTT+TGG - chr4_2:51185247-51185266 Msa0605380:intron 30.0%
TAACCTCAGTAAATTGAAAC+CGG - chr4_2:51185445-51185464 Msa0605380:intron 30.0%
TAATTCACGTGAACAATTAC+CGG + chr4_2:51185467-51185486 None:intergenic 30.0%
ACAACTAAGACATGGAAATA+AGG + chr4_2:51185590-51185609 None:intergenic 30.0%
!! TGTTTTGAACAACTAAGACA+TGG + chr4_2:51185598-51185617 None:intergenic 30.0%
!!! TGTTGTTCTTCTGGTTTTGA+AGG + chr4_2:51182913-51182932 None:intergenic 35.0%
TGAGGATTGTTTCGCATTAA+GGG + chr4_2:51183003-51183022 None:intergenic 35.0%
ATGAGGATTGTTTCGCATTA+AGG + chr4_2:51183004-51183023 None:intergenic 35.0%
GTTGTGATTGGAGTTCTTAT+TGG - chr4_2:51183054-51183073 Msa0605380:three_prime_UTR 35.0%
! TTAGCTTTCTTCTTTCCCAA+TGG - chr4_2:51183084-51183103 Msa0605380:three_prime_UTR 35.0%
TCCCAAAAGTTGTCACAAAA+TGG + chr4_2:51183662-51183681 None:intergenic 35.0%
ACTTGTGGTTACATGTTGTT+GGG - chr4_2:51183858-51183877 Msa0605380:CDS 35.0%
CCTTTCTATCATTCAAGCTT+AGG + chr4_2:51183978-51183997 None:intergenic 35.0%
CCTAAGCTTGAATGATAGAA+AGG - chr4_2:51183975-51183994 Msa0605380:CDS 35.0%
AAGAGACATAATGGCACAAT+TGG - chr4_2:51184019-51184038 Msa0605380:CDS 35.0%
AGAGATTTGATCATCGGATT+TGG + chr4_2:51184045-51184064 None:intergenic 35.0%
AACAGGAGAGATTTGATCAT+CGG + chr4_2:51184051-51184070 None:intergenic 35.0%
!! AAGTTTTATCCCACTAAGTG+GGG + chr4_2:51184116-51184135 None:intergenic 35.0%
!! CAAGTTTTATCCCACTAAGT+GGG + chr4_2:51184117-51184136 None:intergenic 35.0%
ACCCCATTTCAAAAGAAAAC+AGG + chr4_2:51184170-51184189 None:intergenic 35.0%
!! CAAATTATTATTGGTGGCGT+TGG - chr4_2:51184229-51184248 Msa0605380:CDS 35.0%
TTGAGCACTAACACAAACAT+TGG + chr4_2:51184282-51184301 None:intergenic 35.0%
AGTGCTCAATAGTTTATGAG+CGG - chr4_2:51184293-51184312 Msa0605380:intron 35.0%
!!! CTGGTTTTTGGTTTACAAGA+TGG - chr4_2:51184387-51184406 Msa0605380:intron 35.0%
!!! GGTTTTTGGTTTACAAGATG+GGG - chr4_2:51184389-51184408 Msa0605380:intron 35.0%
!! TAACATGTTGACTCTATCTG+AGG + chr4_2:51184496-51184515 None:intergenic 35.0%
AAATGCTGAGCTGAAAAAAC+AGG - chr4_2:51184545-51184564 Msa0605380:intron 35.0%
TTCACCAATATCAAGAGAGT+TGG - chr4_2:51184822-51184841 Msa0605380:intron 35.0%
! CTGGGTCTCTTTTGTAAAAT+GGG + chr4_2:51184914-51184933 None:intergenic 35.0%
! TCTGGGTCTCTTTTGTAAAA+TGG + chr4_2:51184915-51184934 None:intergenic 35.0%
TAGTGTCAATACCATCATCT+GGG + chr4_2:51184932-51184951 None:intergenic 35.0%
ATAGTGTCAATACCATCATC+TGG + chr4_2:51184933-51184952 None:intergenic 35.0%
!! GATGATGGTATTGACACTAT+TGG - chr4_2:51184933-51184952 Msa0605380:intron 35.0%
ACACTATTGGAAAACAAGGA+GGG - chr4_2:51184946-51184965 Msa0605380:intron 35.0%
ATACAATGTCAACGTCTGAT+AGG + chr4_2:51185040-51185059 None:intergenic 35.0%
TCACGGTATAGATGATCAAA+AGG + chr4_2:51185074-51185093 None:intergenic 35.0%
CGTAAGCTGTCATGTTATTA+TGG + chr4_2:51185124-51185143 None:intergenic 35.0%
ATTGTAAACCCATATCCTCA+TGG + chr4_2:51185201-51185220 None:intergenic 35.0%
AGGATATGGGTTTACAATTC+CGG - chr4_2:51185203-51185222 Msa0605380:intron 35.0%
TGGTCTGATATAGAAGAAAC+CGG + chr4_2:51185225-51185244 None:intergenic 35.0%
AGTTCAATTGAAGGAATGGT+TGG + chr4_2:51185245-51185264 None:intergenic 35.0%
AAAGAGTTCCTCGTTGAAAA+CGG + chr4_2:51185318-51185337 None:intergenic 35.0%
!! AAGTTCTTTTCAAGACAGTG+AGG - chr4_2:51185414-51185433 Msa0605380:intron 35.0%
!! TTACCGGTTTCAATTTACTG+AGG + chr4_2:51185451-51185470 None:intergenic 35.0%
! TAGTGATAATGCATAGGTGT+AGG - chr4_2:51185637-51185656 Msa0605380:CDS 35.0%
AACTTGCAGTCTCAAAATGA+GGG + chr4_2:51185708-51185727 None:intergenic 35.0%
AAACTTGCAGTCTCAAAATG+AGG + chr4_2:51185709-51185728 None:intergenic 35.0%
! AGATGGTAGTGTTGTTCTTC+TGG + chr4_2:51182922-51182941 None:intergenic 40.0%
CCTCAAACACTGAATTGAGA+TGG + chr4_2:51182939-51182958 None:intergenic 40.0%
CCATCTCAATTCAGTGTTTG+AGG - chr4_2:51182936-51182955 Msa0605380:three_prime_UTR 40.0%
TCTCAATTCAGTGTTTGAGG+TGG - chr4_2:51182939-51182958 Msa0605380:three_prime_UTR 40.0%
TGGTGGTAGCTACAATGATT+GGG - chr4_2:51182959-51182978 Msa0605380:three_prime_UTR 40.0%
!! CTTGATTTGCGTAGAGAATG+AGG + chr4_2:51183021-51183040 None:intergenic 40.0%
CTCATTCTCTACGCAAATCA+AGG - chr4_2:51183019-51183038 Msa0605380:three_prime_UTR 40.0%
ATTGTTACCGCTGTTGTGAT+TGG - chr4_2:51183042-51183061 Msa0605380:three_prime_UTR 40.0%
AAGAACTCCAATCACAACAG+CGG + chr4_2:51183052-51183071 None:intergenic 40.0%
TGAGAAACGAAGAAGCCATT+GGG + chr4_2:51183102-51183121 None:intergenic 40.0%
TTGAGAAACGAAGAAGCCAT+TGG + chr4_2:51183103-51183122 None:intergenic 40.0%
!! GAGATTTGGGTTGTTAGATG+TGG - chr4_2:51183534-51183553 Msa0605380:CDS 40.0%
!! AACCATTCTGGGACAACTTT+CGG + chr4_2:51183794-51183813 None:intergenic 40.0%
TCTCAAAAGTTTGCGAGTGT+CGG - chr4_2:51183828-51183847 Msa0605380:CDS 40.0%
GACTTGTGGTTACATGTTGT+TGG - chr4_2:51183857-51183876 Msa0605380:CDS 40.0%
TGGTTACATGTTGTTGGGTA+TGG - chr4_2:51183863-51183882 Msa0605380:CDS 40.0%
!! CCAAGTTTTATCCCACTAAG+TGG + chr4_2:51184118-51184137 None:intergenic 40.0%
CCACTTAGTGGGATAAAACT+TGG - chr4_2:51184115-51184134 Msa0605380:CDS 40.0%
TGAGCTGAAAAAACAGGTGA+GGG - chr4_2:51184551-51184570 Msa0605380:intron 40.0%
!!! CAAGAAACTGTTTTTGAGCC+TGG + chr4_2:51184617-51184636 None:intergenic 40.0%
AATCCCTTGACAGTACCAAA+AGG + chr4_2:51184666-51184685 None:intergenic 40.0%
TCTTGGGTTCACAGATTCAT+CGG + chr4_2:51184712-51184731 None:intergenic 40.0%
CCAATATCTTTGCCAGTCTT+GGG + chr4_2:51184728-51184747 None:intergenic 40.0%
TCCAATATCTTTGCCAGTCT+TGG + chr4_2:51184729-51184748 None:intergenic 40.0%
TACAAAAGAGACCCAGATGA+TGG - chr4_2:51184918-51184937 Msa0605380:intron 40.0%
GACACTATTGGAAAACAAGG+AGG - chr4_2:51184945-51184964 Msa0605380:intron 40.0%
TGGACTCAACATCTGAATCA+CGG + chr4_2:51185091-51185110 None:intergenic 40.0%
CGGTCCAAAAGTTCAATTGA+AGG + chr4_2:51185254-51185273 None:intergenic 40.0%
TCATGCTGCTAGAAGAACTA+TGG - chr4_2:51185367-51185386 Msa0605380:intron 40.0%
TCAACAACTGCTTTCATTCG+CGG + chr4_2:51185506-51185525 None:intergenic 40.0%
!! TTTTGGTACTTGCTCTAAGC+TGG - chr4_2:51185765-51185784 Msa0605380:five_prime_UTR 40.0%
! CAATTCAGTGTTTGAGGTGG+TGG - chr4_2:51182942-51182961 Msa0605380:three_prime_UTR 45.0%
GTGGTGGTAGCTACAATGAT+TGG - chr4_2:51182958-51182977 Msa0605380:three_prime_UTR 45.0%
AATCGTCGCATCCATGTTGT+TGG - chr4_2:51183135-51183154 Msa0605380:exon 45.0%
!!! GGGTTTTGGATCCAACAACA+TGG + chr4_2:51183149-51183168 None:intergenic 45.0%
TGTTGTTGGATCCAAAACCC+AGG - chr4_2:51183149-51183168 Msa0605380:CDS 45.0%
! AGTGTCGGATACTTGACTTG+TGG - chr4_2:51183843-51183862 Msa0605380:CDS 45.0%
TTTATCCCACTAAGTGGGGT+CGG + chr4_2:51184112-51184131 None:intergenic 45.0%
!! TTGGTGCTACCTGTGAAAGT+CGG - chr4_2:51184248-51184267 Msa0605380:intron 45.0%
CTGAGCTGAAAAAACAGGTG+AGG - chr4_2:51184550-51184569 Msa0605380:intron 45.0%
! CAAGAAAAAGCCGGACCTTT+TGG - chr4_2:51184648-51184667 Msa0605380:intron 45.0%
CTTGACAGTACCAAAAGGTC+CGG + chr4_2:51184661-51184680 None:intergenic 45.0%
! GGACCTTTTGGTACTGTCAA+GGG - chr4_2:51184660-51184679 Msa0605380:intron 45.0%
ATCGGGAACAACAGTAGGAT+TGG + chr4_2:51184694-51184713 None:intergenic 45.0%
GATTCATCGGGAACAACAGT+AGG + chr4_2:51184699-51184718 None:intergenic 45.0%
CTTGGGTTCACAGATTCATC+GGG + chr4_2:51184711-51184730 None:intergenic 45.0%
TGAATCTGTGAACCCAAGAC+TGG - chr4_2:51184713-51184732 Msa0605380:intron 45.0%
CCCAAGACTGGCAAAGATAT+TGG - chr4_2:51184725-51184744 Msa0605380:intron 45.0%
CTCGAGCATCTCCTTTACAT+TGG + chr4_2:51184796-51184815 None:intergenic 45.0%
CAATGTAAAGGAGATGCTCG+AGG - chr4_2:51184794-51184813 Msa0605380:intron 45.0%
GTTATTATGGCCATCGCTCA+TGG + chr4_2:51185111-51185130 None:intergenic 45.0%
ACGCTCATACCATGAGGATA+TGG - chr4_2:51185189-51185208 Msa0605380:intron 45.0%
CGCTCATACCATGAGGATAT+GGG - chr4_2:51185190-51185209 Msa0605380:intron 45.0%
! TTTTGGACCGTGTAGCAACA+CGG - chr4_2:51185264-51185283 Msa0605380:intron 45.0%
CATGAAGCCCTTCATAACCA+GGG + chr4_2:51185352-51185371 None:intergenic 45.0%
TCATTCGCGGAAGCTTATCA+GGG + chr4_2:51185493-51185512 None:intergenic 45.0%
TTCATTCGCGGAAGCTTATC+AGG + chr4_2:51185494-51185513 None:intergenic 45.0%
GTTACCAATCTGAGCCATCA+GGG + chr4_2:51185568-51185587 None:intergenic 45.0%
ATTGGGCGCAGAGAAAGAGA+AGG - chr4_2:51182976-51182995 Msa0605380:three_prime_UTR 50.0%
GTCCGAAAGTTGTCCCAGAA+TGG - chr4_2:51183789-51183808 Msa0605380:CDS 50.0%
TTTGAACCGACCCCACTTAG+TGG - chr4_2:51184103-51184122 Msa0605380:CDS 50.0%
TTGAACCGACCCCACTTAGT+GGG - chr4_2:51184104-51184123 Msa0605380:CDS 50.0%
!! GACACAGTACCGACTTTCAC+AGG + chr4_2:51184260-51184279 None:intergenic 50.0%
CACATTCAGCTGAGTTTGCC+TGG + chr4_2:51184470-51184489 None:intergenic 50.0%
! CGGACCTTTTGGTACTGTCA+AGG - chr4_2:51184659-51184678 Msa0605380:intron 50.0%
TAAAGGAGATGCTCGAGGTC+TGG - chr4_2:51184799-51184818 Msa0605380:intron 50.0%
ATTTAAGCCCGGAGACAGCA+TGG + chr4_2:51184974-51184993 None:intergenic 50.0%
GATGTTGAGTCCATGAGCGA+TGG - chr4_2:51185098-51185117 Msa0605380:intron 50.0%
CTCGATACGCTCATACCATG+AGG - chr4_2:51185183-51185202 Msa0605380:intron 50.0%
! TGGAAAGCCGTGTTGCTACA+CGG + chr4_2:51185274-51185293 None:intergenic 50.0%
TGTAGCAACACGGCTTTCCA+AGG - chr4_2:51185274-51185293 Msa0605380:intron 50.0%
GTTCCTCGTTGAAAACGGCT+TGG + chr4_2:51185313-51185332 None:intergenic 50.0%
! CTCTTTTATCCGTCACACCC+TGG - chr4_2:51185332-51185351 Msa0605380:intron 50.0%
CCTTCATAACCAGGGTGTGA+CGG + chr4_2:51185344-51185363 None:intergenic 50.0%
CGTCACACCCTGGTTATGAA+GGG - chr4_2:51185342-51185361 Msa0605380:intron 50.0%
GCATGAAGCCCTTCATAACC+AGG + chr4_2:51185353-51185372 None:intergenic 50.0%
GCTCTCAAACCTTTCCCTGA+TGG - chr4_2:51185551-51185570 Msa0605380:intron 50.0%
CAATCTGAGCCATCAGGGAA+AGG + chr4_2:51185563-51185582 None:intergenic 50.0%
CTTTCCCTGATGGCTCAGAT+TGG - chr4_2:51185561-51185580 Msa0605380:CDS 50.0%
GGTTACCAATCTGAGCCATC+AGG + chr4_2:51185569-51185588 None:intergenic 50.0%
AAGTGGGTAGGTCACTCGAG+CGG - chr4_2:51183902-51183921 Msa0605380:CDS 55.0%
AGCGGGTGGGTATGGTTCAT+AGG - chr4_2:51183920-51183939 Msa0605380:CDS 55.0%
AGGTGAGGGAGTTGACCGAA+AGG - chr4_2:51184565-51184584 Msa0605380:intron 55.0%
GGGAGTTGACCGAAAGGCTT+CGG - chr4_2:51184571-51184590 Msa0605380:intron 55.0%
CTCTGCAAGCCGAAGCCTTT+CGG + chr4_2:51184583-51184602 None:intergenic 55.0%
TGCAGAGCAAGGGAAAGACC+AGG - chr4_2:51184596-51184615 Msa0605380:intron 55.0%
TGTCTTCGACGAACCTGCGA+TGG - chr4_2:51185154-51185173 Msa0605380:intron 55.0%
GTCTTCGACGAACCTGCGAT+GGG - chr4_2:51185155-51185174 Msa0605380:intron 55.0%
AGCAACACGGCTTTCCAAGG+AGG - chr4_2:51185277-51185296 Msa0605380:intron 55.0%
GCTTTCCAAGGAGGTCGCAT+GGG - chr4_2:51185286-51185305 Msa0605380:intron 55.0%
!! GGACCAAGCCGTTTTCAACG+AGG - chr4_2:51185307-51185326 Msa0605380:intron 55.0%
CCGTCACACCCTGGTTATGA+AGG - chr4_2:51185341-51185360 Msa0605380:intron 55.0%
TTCGCGGAAGCTTATCAGGG+TGG + chr4_2:51185490-51185509 None:intergenic 55.0%
AGTGGGTAGGTCACTCGAGC+GGG - chr4_2:51183903-51183922 Msa0605380:CDS 60.0%
! AGGCTTCGGCTTGCAGAGCA+AGG - chr4_2:51184585-51184604 Msa0605380:intron 60.0%
! GGCTTCGGCTTGCAGAGCAA+GGG - chr4_2:51184586-51184605 Msa0605380:intron 60.0%
GGAGGGAACCATGCTGTCTC+CGG - chr4_2:51184963-51184982 Msa0605380:intron 60.0%
GAGGGAACCATGCTGTCTCC+GGG - chr4_2:51184964-51184983 Msa0605380:intron 60.0%
TCGACGAACCTGCGATGGGT+TGG - chr4_2:51185159-51185178 Msa0605380:intron 60.0%
! CGACGAACCTGCGATGGGTT+GGG - chr4_2:51185160-51185179 Msa0605380:intron 60.0%
TATCGAGCCCAACCCATCGC+AGG + chr4_2:51185170-51185189 None:intergenic 60.0%
GGCTTTCCAAGGAGGTCGCA+TGG - chr4_2:51185285-51185304 Msa0605380:intron 60.0%
TTGGTCCCATGCGACCTCCT+TGG + chr4_2:51185294-51185313 None:intergenic 60.0%
GGTAGGTCACTCGAGCGGGT+GGG - chr4_2:51183907-51183926 Msa0605380:CDS 65.0%
GTCACTCGAGCGGGTGGGTA+TGG - chr4_2:51183912-51183931 Msa0605380:CDS 65.0%
GGGTAGGTCACTCGAGCGGG+TGG - chr4_2:51183906-51183925 Msa0605380:CDS 70.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 51182867 51185860 51182867 ID=Msa0605380;Name=Msa0605380
chr4_2 mRNA 51182867 51185860 51182867 ID=Msa0605380-mRNA-1;Parent=Msa0605380;Name=Msa0605380-mRNA-1;_AED=0.02;_eAED=0.02;_QI=106|1|1|1|1|1|2|274|437
chr4_2 exon 51182867 51184256 51182867 ID=Msa0605380-mRNA-1:exon:12248;Parent=Msa0605380-mRNA-1
chr4_2 exon 51185557 51185860 51185557 ID=Msa0605380-mRNA-1:exon:12247;Parent=Msa0605380-mRNA-1
chr4_2 five_prime_UTR 51185755 51185860 51185755 ID=Msa0605380-mRNA-1:five_prime_utr;Parent=Msa0605380-mRNA-1
chr4_2 CDS 51185557 51185754 51185557 ID=Msa0605380-mRNA-1:cds;Parent=Msa0605380-mRNA-1
chr4_2 CDS 51183141 51184256 51183141 ID=Msa0605380-mRNA-1:cds;Parent=Msa0605380-mRNA-1
chr4_2 three_prime_UTR 51182867 51183140 51182867 ID=Msa0605380-mRNA-1:three_prime_utr;Parent=Msa0605380-mRNA-1
Gene Sequence

>Msa0605380

ATGATTGGGCGCAGAGAAAGAGAAGGACCCTTAATGCGAAACAATCCTCATTCTCTACGCAAATCAAGGATTGTTACCGCTGTTGTGATTGGAGTTCTTATTGGATGCATCTTAGCTTTCTTCTTTCCCAATGGCTTCTTCGTTTCTCAATCTATTGCATCAAATCGTCGCATCCATGTTGTTGGATCCAAAACCCAGGCAAACTCAGCTGAATGTGAATCCTCAGATAGAGTCAACATGTTAAAATCAGAGTTTGTGGCAGTATCAGATAAAAATGCTGAGCTGAAAAAACAGGTGAGGGAGTTGACCGAAAGGCTTCGGCTTGCAGAGCAAGGGAAAGACCAGGCTCAAAAACAGTTTCTTGCATTAGGTAAACAAGAAAAAGCCGGACCTTTTGGTACTGTCAAGGGATTAAGAACCAATCCTACTGTTGTTCCCGATGAATCTGTGAACCCAAGACTGGCAAAGATATTGGAGAAAAACGCAGTTAAAAGAGAGATCATAGTTGCACTTGCAAATTCCAATGTAAAGGAGATGCTCGAGGTCTGGTTCACCAATATCAAGAGAGTTGGTATAACTAATTATCTAGTTGTTGCTTTAGACGATGAGATTTCGAAGTTTTGTGAATCGAATCAAGTCCCATTTTACAAAAGAGACCCAGATGATGGTATTGACACTATTGGAAAACAAGGAGGGAACCATGCTGTCTCCGGGCTTAAATTTCGTATTCTAAGAGAATTTTTGCAGTTGGGATATAGTGTTCTCCTATCAGACGTTGACATTGTATATTTGCAGAATCCTTTTGATCATCTATACCGTGATTCAGATGTTGAGTCCATGAGCGATGGCCATAATAACATGACAGCTTACGGCTATAACGATGTCTTCGACGAACCTGCGATGGGTTGGGCTCGATACGCTCATACCATGAGGATATGGGTTTACAATTCCGGTTTCTTCTATATCAGACCAACCATTCCTTCAATTGAACTTTTGGACCGTGTAGCAACACGGCTTTCCAAGGAGGTCGCATGGGACCAAGCCGTTTTCAACGAGGAACTCTTTTATCCGTCACACCCTGGTTATGAAGGGCTTCATGCTGCTAGAAGAACTATGGATATGTATCTCTTTATGAACAGCAAAGTTCTTTTCAAGACAGTGAGGAACGATGCTAACCTCAGTAAATTGAAACCGGTAATTGTTCACGTGAATTACCACCCTGATAAGCTTCCGCGAATGAAAGCAGTTGTTGAATATTATGTCAATGGGAAACAAGACGCTCTCAAACCTTTCCCTGATGGCTCAGATTGGTAA
Protein sequence

>Msa0605380

MIGRREREGPLMRNNPHSLRKSRIVTAVVIGVLIGCILAFFFPNGFFVSQSIASNRRIHVVGSKTQANSAECESSDRVNMLKSEFVAVSDKNAELKKQVRELTERLRLAEQGKDQAQKQFLALGKQEKAGPFGTVKGLRTNPTVVPDESVNPRLAKILEKNAVKREIIVALANSNVKEMLEVWFTNIKRVGITNYLVVALDDEISKFCESNQVPFYKRDPDDGIDTIGKQGGNHAVSGLKFRILREFLQLGYSVLLSDVDIVYLQNPFDHLYRDSDVESMSDGHNNMTAYGYNDVFDEPAMGWARYAHTMRIWVYNSGFFYIRPTIPSIELLDRVATRLSKEVAWDQAVFNEELFYPSHPGYEGLHAARRTMDMYLFMNSKVLFKTVRNDANLSKLKPVIVHVNYHPDKLPRMKAVVEYYVNGKQDALKPFPDGSDW*