AlfalfaGEDB | Msa0130710

M. sativa cultivar zhongmu-4 / Msa0130710

KEGG: -
GO: GO:0000976 GO:0000981 GO:0001046 GO:0001047 GO:0001067 GO:0001228 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032774 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:0090304 GO:0097159 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0130710	sp\|Q9FYJ6\|BH111_ARATH	51.208	207	86	6	335	537	124	319	1.28e-49	176

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0130710	A0A072VPZ1	97.579	537	9	1	1	537	1	533	0.0	1087

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0011040	Msa0130710	0.813091	3.051002e-51	-8.615850e-47
Msa0012800	Msa0130710	0.830631	2.633478e-55	-8.615850e-47
Msa0017710	Msa0130710	0.808200	3.480221e-50	-8.615850e-47
Msa0019570	Msa0130710	0.810495	1.120439e-50	-8.615850e-47
Msa0024650	Msa0130710	0.822216	2.666694e-53	-8.615850e-47
Msa0034100	Msa0130710	-0.822415	2.398057e-53	-8.615850e-47
Msa0036550	Msa0130710	0.951033	4.784620e-109	-8.615850e-47
Msa0038010	Msa0130710	0.826451	2.692926e-54	-8.615850e-47
Msa0041270	Msa0130710	0.817818	2.707601e-52	-8.615850e-47
Msa0041780	Msa0130710	-0.800389	1.474678e-48	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0130710	MtrunA17_Chr1g0199091	97.579	537	9	1	1	537	1	533	0.0	1087
Msa0130710	MtrunA17_Chr6g0486111	45.223	157	57	4	403	533	167	320	1.34e-31	124
Msa0130710	MtrunA17_Chr2g0280631	48.649	148	66	5	394	536	272	414	8.30e-31	124
Msa0130710	MtrunA17_Chr8g0361501	45.625	160	80	4	377	535	290	443	9.56e-30	121
Msa0130710	MtrunA17_Chr2g0318971	50.769	130	61	2	404	533	308	434	1.35e-29	121
Msa0130710	MtrunA17_Chr8g0390301	42.857	161	55	4	403	533	180	333	1.94e-28	115
Msa0130710	MtrunA17_Chr2g0331431	44.375	160	55	5	403	531	169	325	2.07e-28	115
Msa0130710	MtrunA17_Chr1g0207771	39.130	184	89	6	361	533	102	273	1.67e-27	112
Msa0130710	MtrunA17_Chr3g0141841	47.101	138	68	3	384	517	299	435	5.89e-27	113
Msa0130710	MtrunA17_Chr4g0045561	50.000	102	41	3	428	529	59	150	1.21e-21	92.0

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0130710	AT1G31050.6	51.208	207	90	5	335	537	239	438	5.62e-52	183
Msa0130710	AT1G31050.1	51.208	207	86	6	335	537	239	434	1.85e-50	179
Msa0130710	AT1G31050.7	51.232	203	84	6	335	533	239	430	2.31e-48	173
Msa0130710	AT1G31050.8	43.961	207	79	7	335	537	239	412	3.41e-35	137
Msa0130710	AT3G19500.1	49.612	129	57	5	409	533	135	259	5.81e-27	110
Msa0130710	AT1G27660.1	49.565	115	53	2	402	513	311	423	7.05e-27	114
Msa0130710	AT1G61660.7	42.775	173	84	5	377	537	224	393	3.51e-26	110
Msa0130710	AT1G61660.1	42.775	173	84	5	377	537	224	393	3.51e-26	110
Msa0130710	AT3G20640.1	37.363	182	89	5	355	535	296	453	3.97e-26	111
Msa0130710	AT3G20640.4	37.912	182	88	5	355	535	234	391	4.27e-26	110

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Find 129 sgRNAs with CRISPR-Local

Find 231 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATAATTTATAGAGGTGT+TGG	0.410268	1_3:-61117615	Msa0130710:intron
AAATTCTTCCTCTTCTTGTG+AGG	0.543566	1_3:-61118414	Msa0130710:CDS
AACAAAGACCTTTACTTCGA+AGG	0.532863	1_3:+61114646	None:intergenic
AACAACAAATTCATCTTCAA+AGG	0.487475	1_3:-61116255	Msa0130710:intron
AACAGTGAGGCTTGAATGGT+TGG	0.481356	1_3:+61118349	None:intergenic
AACCACTAATGGAAGAGGAG+AGG	0.625336	1_3:-61116357	Msa0130710:CDS
AACCCATCAAGAAATCCATT+TGG	0.394816	1_3:+61117021	None:intergenic
AACTCAGTGGGAGATCACAA+TGG	0.686157	1_3:-61116985	Msa0130710:CDS
AAGAATTTGGATTATTCATT+TGG	0.300698	1_3:+61118429	None:intergenic
AAGATGAGTGAAGAATGTGG+TGG	0.756172	1_3:-61118551	Msa0130710:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTAGTGATTCCTTTAATTGG+TGG	+	chr1_3:61114453-61114472	None:intergenic	30.0%
ATAAAGATGAGTGAAGAATG+TGG	-	chr1_3:61114509-61114528	Msa0130710:three_prime_UTR	30.0%
ATAAAACATGACTCCATAGT+TGG	+	chr1_3:61114820-61114839	None:intergenic	30.0%
AAGTTAGGTATATAGGGTTA+AGG	-	chr1_3:61115079-61115098	Msa0130710:intron	30.0%
TGTTAGCATGTTATAGATAG+TGG	+	chr1_3:61115160-61115179	None:intergenic	30.0%
ATATCTTACAGAATCCAAGA+GGG	+	chr1_3:61115282-61115301	None:intergenic	30.0%
TATATCTTACAGAATCCAAG+AGG	+	chr1_3:61115283-61115302	None:intergenic	30.0%
TTGCACAATAACCAAGAAAT+TGG	-	chr1_3:61115484-61115503	Msa0130710:intron	30.0%
AGAAATTCTCTCCAATTTCT+TGG	+	chr1_3:61115498-61115517	None:intergenic	30.0%
AGAAATTGGAGAGAATTTCT+TGG	-	chr1_3:61115498-61115517	Msa0130710:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_3	gene	61114438	61118647	61114438	ID=Msa0130710;Name=Msa0130710
chr1_3	mRNA	61114438	61118647	61114438	ID=Msa0130710-mRNA-1;Parent=Msa0130710;Name=Msa0130710-mRNA-1;_AED=0.07;_eAED=0.07;_QI=77\|1\|1\|1\|1\|1\|7\|116\|537
chr1_3	exon	61114438	61114721	61114438	ID=Msa0130710-mRNA-1:exon:20221;Parent=Msa0130710-mRNA-1
chr1_3	exon	61114851	61114889	61114851	ID=Msa0130710-mRNA-1:exon:20220;Parent=Msa0130710-mRNA-1
chr1_3	exon	61115837	61115902	61115837	ID=Msa0130710-mRNA-1:exon:20219;Parent=Msa0130710-mRNA-1
chr1_3	exon	61116047	61116118	61116047	ID=Msa0130710-mRNA-1:exon:20218;Parent=Msa0130710-mRNA-1
chr1_3	exon	61116256	61116384	61116256	ID=Msa0130710-mRNA-1:exon:20217;Parent=Msa0130710-mRNA-1
chr1_3	exon	61116810	61117623	61116810	ID=Msa0130710-mRNA-1:exon:20216;Parent=Msa0130710-mRNA-1
chr1_3	exon	61118245	61118647	61118245	ID=Msa0130710-mRNA-1:exon:20215;Parent=Msa0130710-mRNA-1
chr1_3	five_prime_UTR	61118571	61118647	61118571	ID=Msa0130710-mRNA-1:five_prime_utr;Parent=Msa0130710-mRNA-1
chr1_3	CDS	61118245	61118570	61118245	ID=Msa0130710-mRNA-1:cds;Parent=Msa0130710-mRNA-1
chr1_3	CDS	61116810	61117623	61116810	ID=Msa0130710-mRNA-1:cds;Parent=Msa0130710-mRNA-1
chr1_3	CDS	61116256	61116384	61116256	ID=Msa0130710-mRNA-1:cds;Parent=Msa0130710-mRNA-1
chr1_3	CDS	61116047	61116118	61116047	ID=Msa0130710-mRNA-1:cds;Parent=Msa0130710-mRNA-1
chr1_3	CDS	61115837	61115902	61115837	ID=Msa0130710-mRNA-1:cds;Parent=Msa0130710-mRNA-1
chr1_3	CDS	61114851	61114889	61114851	ID=Msa0130710-mRNA-1:cds;Parent=Msa0130710-mRNA-1
chr1_3	CDS	61114554	61114721	61114554	ID=Msa0130710-mRNA-1:cds;Parent=Msa0130710-mRNA-1
chr1_3	three_prime_UTR	61114438	61114553	61114438	ID=Msa0130710-mRNA-1:three_prime_utr;Parent=Msa0130710-mRNA-1

Gene Sequence

>Msa0130710

ATGAGTGAAGAATGTGGTGGAAACACTATTGCTACCTCTACTACTCCATTAAACTGGTGGTATCTTCAAGCAAATTCTCTTTCTTCAAATTGGAACGATGTTAAACATGCATGGAACAACCAAATGAATAATCCAAATTCTTCCTCTTCTTGTGAGGATCAAGATATATCTGTTTCTTCTACATCTTTCACTAATGCATCCAACCATTCAAGCCTCACTGTTGAATCCTCTCGAAGAGTGTTCGTCGAGCCACATGCACCACCTTCCTCCAACGACTTCATGGCCGAACATGCTTCGGATAACCAACTATGGAGTCATGTTTTATCAGGTGTTGGATCAAATGGAGAGTTGCACAATAACCAAGAAATTGGAGAGAATTTCTTGGATGGACTATCATCAAAAACCATGACAAACACTATGTTTGATCAACCAGCTTGTGACTATTTGAAGAAACTTGATACAACAAGTTGGGAATATAATACTAGTGGATCAACTTCATTCAATACTAATTTTGAGAAGCACTTAAATGGGTACAGTGAAGCTCTTATTGAGAATAATGAAAGGTTGACAAAGTTATCCAATCTTGTAAGCACTTGGTCTATTGCCCCACCTGACCCTGAAGTTAGTAGCCACTTTGATTCACAAACAAACAACAATTTGTCTACTAATTTGAATAATTCTAATTCCTCCAATATGGATCATCATCAATACACACATGATTCTGACCCTAATTGTCATTTCAAACTACCTTTTGGAGATTCCACATCATGCACTATTAGAGAAGGAGTAGGTAATAAAATGTTCCCTAGTTGCCATGATATTCAAGACATGAATAAGGTTAAACAAGAATATCATCATCATGCAAGTGAAGTGATGCAAGGAAATGTGTTTAGAAAATCACTTAATCCAAATGGATTTCTTGATGGGTTTAATAATAGCCTTAACTCAGTGGGAGATCACAATGGCAAATTTTATGAAGGGTTGCCAAATATTTCACCATGCACCAAAAATTTCTCAGATGTTATATCCTTTAATAGTAGGCTTGGAAGGCCAGTTGTTGGAATCCATCCACAGAGGCCTAATATGAGATACTCAAACTTATCTGAGTCAAAGAAACAAGGGCTTCAGACACCATCCCATATGAGAACCACTAATGGAAGAGGAGAGGGAACAACACGTGAAGTAAAGAAGAAAAGATCTGAAGAATCATCAGAAGGAAGTTTGAAGAAACCTAAGCAAGATACTTCAACAACAAATTCATCTTCAAAGGTGCAAGCACCTAAAGTCAAGCTAGGAGACAAGATCACAGCCCTTCAACAAATTGTGTCTCCATTTGGAAAGACTGATACAGCATCTGTGCTGTTTGAAGCAATAGGGTACATAAAGTATCTTGAAGAGCAAGTCCAGTTACTGAGCAACCCTTACTTGAAGGCAAATTCACACAAGGATCCAAGGGGAAGCTACTTCGATAGAAAAGACAAGGAGGATGCAAAGCTAGACCTTCGAAGTAAAGGTCTTTGTTTAGTTCCTACGTCATGTACCCCTATAGTTTATAGAGAGAACAATGGACCAGATTACTGGACACCAGCGTATAGAGGATGTATGTATAGGTGA

Protein sequence

>Msa0130710

MSEECGGNTIATSTTPLNWWYLQANSLSSNWNDVKHAWNNQMNNPNSSSSCEDQDISVSSTSFTNASNHSSLTVESSRRVFVEPHAPPSSNDFMAEHASDNQLWSHVLSGVGSNGELHNNQEIGENFLDGLSSKTMTNTMFDQPACDYLKKLDTTSWEYNTSGSTSFNTNFEKHLNGYSEALIENNERLTKLSNLVSTWSIAPPDPEVSSHFDSQTNNNLSTNLNNSNSSNMDHHQYTHDSDPNCHFKLPFGDSTSCTIREGVGNKMFPSCHDIQDMNKVKQEYHHHASEVMQGNVFRKSLNPNGFLDGFNNSLNSVGDHNGKFYEGLPNISPCTKNFSDVISFNSRLGRPVVGIHPQRPNMRYSNLSESKKQGLQTPSHMRTTNGRGEGTTREVKKKRSEESSEGSLKKPKQDTSTTNSSSKVQAPKVKLGDKITALQQIVSPFGKTDTASVLFEAIGYIKYLEEQVQLLSNPYLKANSHKDPRGSYFDRKDKEDAKLDLRSKGLCLVPTSCTPIVYRENNGPDYWTPAYRGCMYR*