AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0686530


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0686530 MtrunA17_Chr4g0035251 97.333 300 8 0 2 301 4 303 0.0 592
Msa0686530 MtrunA17_Chr4g0035261 58.000 300 114 6 8 301 8 301 7.55e-110 320
Msa0686530 MtrunA17_Chr3g0144781 55.814 301 119 7 6 301 5 296 8.91e-102 300
Msa0686530 MtrunA17_Chr3g0144751 56.333 300 121 6 6 301 5 298 2.58e-99 293
Msa0686530 MtrunA17_Chr4g0035231 43.253 289 154 4 16 301 15 296 5.94e-63 201
Msa0686530 MtrunA17_Chr4g0035221 41.176 289 160 4 16 301 15 296 9.80e-60 192
Msa0686530 MtrunA17_Chr8g0370921 34.576 295 182 6 10 300 17 304 9.84e-47 159
Msa0686530 MtrunA17_Chr5g0406531 31.525 295 185 8 11 301 3 284 2.12e-39 139
Msa0686530 MtrunA17_Chr8g0370891 32.534 292 186 6 13 300 5 289 7.77e-39 138
Msa0686530 MtrunA17_Chr8g0370901 29.655 290 195 5 13 300 5 287 8.15e-39 138
Msa0686530 MtrunA17_Chr5g0406361 30.573 314 198 9 1 300 7 314 6.33e-38 136
Msa0686530 MtrunA17_Chr5g0406421 30.847 295 187 8 9 299 2 283 1.07e-37 135
Msa0686530 MtrunA17_Chr5g0406601 31.081 296 185 9 9 299 2 283 2.31e-37 134
Msa0686530 MtrunA17_Chr5g0406411 30.847 295 187 8 9 299 2 283 6.04e-37 133
Msa0686530 MtrunA17_Chr8g0370881 30.479 292 192 6 13 300 5 289 6.70e-37 133
Msa0686530 MtrunA17_Chr5g0406391 30.612 294 189 6 9 299 2 283 7.07e-37 133
Msa0686530 MtrunA17_Chr5g0406501 30.872 298 184 11 11 301 3 285 9.43e-36 130
Msa0686530 MtrunA17_Chr5g0406401 29.831 295 190 8 9 299 2 283 1.35e-35 129
Msa0686530 MtrunA17_Chr5g0406511 30.743 296 190 8 7 299 1 284 1.96e-35 129
Msa0686530 MtrunA17_Chr5g0406561 29.392 296 194 7 9 299 2 287 7.55e-35 127
Msa0686530 MtrunA17_Chr5g0406481 30.743 296 190 8 7 299 1 284 1.24e-34 127
Msa0686530 MtrunA17_Chr5g0406371 30.537 298 182 11 9 299 2 281 1.88e-34 126
Msa0686530 MtrunA17_Chr8g0370861 31.596 307 175 9 4 301 3 283 5.97e-28 109
Msa0686530 MtrunA17_Chr5g0406631 30.519 308 189 7 9 301 8 305 9.59e-23 95.9
Msa0686530 MtrunA17_Chr1g0164671 32.900 231 126 8 72 301 24 226 6.82e-21 89.0
Msa0686530 MtrunA17_Chr5g0406471 31.313 198 119 6 105 301 4 185 4.77e-19 83.2
Msa0686530 MtrunA17_Chr3g0144771 48.684 76 39 0 1 76 1 76 3.43e-13 63.9
Msa0686530 MtrunA17_Chr3g0143741 26.144 306 197 11 9 299 23 314 3.74e-13 68.9
Msa0686530 MtrunA17_Chr5g0406441 32.673 101 66 1 10 108 2 102 1.95e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0686530 AT2G17080.1 40.625 288 137 9 16 301 8 263 4.09e-51 169
Msa0686530 AT2G17070.1 38.889 288 142 8 16 301 8 263 5.35e-51 169
Msa0686530 AT4G35210.1 39.223 283 132 9 16 298 8 250 4.34e-48 161
Msa0686530 AT4G35200.1 39.576 283 134 9 16 298 8 253 2.51e-47 159
Msa0686530 AT4G35690.1 34.783 299 163 11 13 301 8 284 4.20e-42 147
Msa0686530 AT4G35710.1 31.313 297 173 9 14 301 9 283 5.34e-29 112
Msa0686530 AT2G17680.1 29.967 307 167 11 16 300 11 291 1.05e-27 109
Msa0686530 AT4G35720.1 28.308 325 196 11 7 300 6 324 1.66e-20 90.1
Msa0686530 AT4G35680.1 29.299 314 197 11 4 301 6 310 5.93e-20 88.6
Msa0686530 AT1G76210.1 29.150 247 144 6 57 301 9 226 5.34e-19 84.3
Msa0686530 AT3G51400.1 29.902 204 129 4 100 300 82 274 1.10e-18 84.3
Msa0686530 AT4G35660.1 27.667 300 185 7 9 299 10 286 3.94e-15 74.7
Msa0686530 AT1G20520.1 26.506 249 152 6 54 300 6 225 8.94e-13 67.0

Find 49 sgRNAs with CRISPR-Local

Find 120 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAGAACCAATTGGAAAATT+TGG 0.112535 4_4:-51038948 Msa0686530:CDS
TGAGAAAATTCTTGGCTTCT+AGG 0.199154 4_4:-51039333 Msa0686530:CDS
ATTTATACAACTAGTGATTA+AGG 0.290293 4_4:+51038843 None:intergenic
AATCGAAACCAAGTAATTAT+TGG 0.313179 4_4:-51039114 Msa0686530:CDS
GGCCGAGGTGAGAAAATTCT+TGG 0.322610 4_4:-51039341 Msa0686530:CDS
ACATGATTGCGTCGAAAGTT+TGG 0.329591 4_4:-51039557 Msa0686530:CDS
TGATGTGATGTTGGACTAAT+AGG 0.335874 4_4:+51039721 None:intergenic
ATTATCTCCTAGCAACAATA+AGG 0.373881 4_4:-51039236 Msa0686530:CDS
TCAAATTCTCCAAAGCTTTG+AGG 0.381031 4_4:+51039290 None:intergenic
CAATCATTTGCAGAACCAAT+TGG 0.396017 4_4:-51038957 Msa0686530:CDS
GAAAGCAATCCTCAAAGCTT+TGG 0.399242 4_4:-51039299 Msa0686530:CDS
CAAAGCTTTGGAGAATTTGA+AGG 0.400637 4_4:-51039287 Msa0686530:CDS
ACCAAGGACCAATAATTACT+TGG 0.401947 4_4:+51039106 None:intergenic
TCCACGCACGTCAAGAGAAA+TGG 0.417810 4_4:-51039504 Msa0686530:CDS
ACCAAGTAATTATTGGTCCT+TGG 0.430617 4_4:-51039107 Msa0686530:CDS
TTTAATCTTTATATCTGGAC+CGG 0.469654 4_4:-51039140 Msa0686530:CDS
ATCAACTCTTCATCGTTGTT+CGG 0.473180 4_4:-51039607 Msa0686530:CDS
TTGCTACAGAACACACATCT+AGG 0.484586 4_4:+51039449 None:intergenic
GTCTGTTGTTCAAGGGTTTG+TGG 0.488801 4_4:-51038924 Msa0686530:CDS
AGTGATCACTCTTTCAATGT+TGG 0.496374 4_4:-51039170 Msa0686530:CDS
ACAAGGACTTGTTGAGTAAG+AGG 0.502400 4_4:+51039526 None:intergenic
ATTTGGAGTCTGTTGTTCAA+GGG 0.504312 4_4:-51038931 Msa0686530:CDS
CGAAATGGAGGTGACGGCCG+AGG 0.520093 4_4:-51039356 Msa0686530:CDS
AAAATTCTTGGCTTCTAGGA+AGG 0.521847 4_4:-51039329 Msa0686530:CDS
TCTCTTGACGTGCGTGGACA+AGG 0.524756 4_4:+51039509 None:intergenic
TCATGATCCTTATTGTTGCT+AGG 0.526232 4_4:+51039229 None:intergenic
TTGAAGGCTAATGTGAAGAA+AGG 0.526883 4_4:-51039271 Msa0686530:CDS
GCTAATGTGAAGAAAGGTAA+AGG 0.528831 4_4:-51039265 Msa0686530:CDS
GCTTATGATGCAGAACAAGA+AGG 0.533958 4_4:-51039077 Msa0686530:CDS
AATTTGGAGTCTGTTGTTCA+AGG 0.538470 4_4:-51038932 Msa0686530:CDS
TCCATTTCTCTTGACGTGCG+TGG 0.539554 4_4:+51039503 None:intergenic
CATTGTAGAATGAGAGGGTG+TGG 0.553149 4_4:+51039667 None:intergenic
CGAGGTTGGTGATGTGATGT+TGG 0.563264 4_4:+51039712 None:intergenic
AGGAGGCGAAATGGAGGTGA+CGG 0.564930 4_4:-51039362 Msa0686530:CDS
ATGAGAGGGTGTGGTTTCGA+TGG 0.575082 4_4:+51039676 None:intergenic
GAAGAGTTGATTGCGTCGAA+AGG 0.582245 4_4:+51039619 None:intergenic
TCTACAATGCAATCAACACT+TGG 0.582703 4_4:-51039653 Msa0686530:CDS
AGATGTGTGTTCTGTAGCAA+AGG 0.585332 4_4:-51039446 Msa0686530:CDS
CATCATAAGCTTTGAAACCA+AGG 0.589484 4_4:+51039090 None:intergenic
AGTGATTAAGGACATTGAGA+AGG 0.590750 4_4:+51038855 None:intergenic
GTGATTAAGGACATTGAGAA+GGG 0.597371 4_4:+51038856 None:intergenic
TTACTTGGTTTCGATTGCGC+CGG 0.602841 4_4:+51039121 None:intergenic
GGTTAGTTTGTTAAGAGACG+TGG 0.607951 4_4:-51039194 Msa0686530:CDS
TGATTGCATTGTAGAATGAG+AGG 0.610266 4_4:+51039661 None:intergenic
ATCATGTAAAGCTTGTAGAT+CGG 0.622289 4_4:+51039573 None:intergenic
GGATTCTCTAATTCATACGA+AGG 0.644514 4_4:-51039425 Msa0686530:CDS
TGATGTTGGACTAATAGGAA+TGG 0.654192 4_4:+51039726 None:intergenic
AGATCCGTCTAAAAGCTCAT+CGG 0.663380 4_4:+51039480 None:intergenic
GATTGCATTGTAGAATGAGA+GGG 0.668472 4_4:+51039662 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCACTTTTAATCTTTATATC+TGG - chr4_4:51039444-51039463 Msa0686530:CDS 20.0%
!!! TCACTTTTAATCTTTATATC+TGG - chr4_4:51039444-51039463 Msa0686530:CDS 20.0%
! TTTAATCTTTATATCTGGAC+CGG - chr4_4:51039449-51039468 Msa0686530:CDS 25.0%
! AATCGAAACCAAGTAATTAT+TGG - chr4_4:51039475-51039494 Msa0686530:CDS 25.0%
! TTTAATCTTTATATCTGGAC+CGG - chr4_4:51039449-51039468 Msa0686530:CDS 25.0%
! AATCGAAACCAAGTAATTAT+TGG - chr4_4:51039475-51039494 Msa0686530:CDS 25.0%
ATCATGTAAAGCTTGTAGAT+CGG + chr4_4:51039019-51039038 None:intergenic 30.0%
ATTATCTCCTAGCAACAATA+AGG - chr4_4:51039353-51039372 Msa0686530:CDS 30.0%
ATCATGTAAAGCTTGTAGAT+CGG + chr4_4:51039019-51039038 None:intergenic 30.0%
ATTATCTCCTAGCAACAATA+AGG - chr4_4:51039353-51039372 Msa0686530:CDS 30.0%
TGATGTTGGACTAATAGGAA+TGG + chr4_4:51038866-51038885 None:intergenic 35.0%
TGATGTGATGTTGGACTAAT+AGG + chr4_4:51038871-51038890 None:intergenic 35.0%
GATTGCATTGTAGAATGAGA+GGG + chr4_4:51038930-51038949 None:intergenic 35.0%
TGATTGCATTGTAGAATGAG+AGG + chr4_4:51038931-51038950 None:intergenic 35.0%
TCTACAATGCAATCAACACT+TGG - chr4_4:51038936-51038955 Msa0686530:CDS 35.0%
ATCAACTCTTCATCGTTGTT+CGG - chr4_4:51038982-51039001 Msa0686530:CDS 35.0%
! AGCTTTTAGACGGATCTTTA+AGG - chr4_4:51039115-51039134 Msa0686530:CDS 35.0%
GGATTCTCTAATTCATACGA+AGG - chr4_4:51039164-51039183 Msa0686530:CDS 35.0%
TCGATTATGCGAAGAAAAAG+AGG - chr4_4:51039207-51039226 Msa0686530:CDS 35.0%
ATTATGCGAAGAAAAAGAGG+AGG - chr4_4:51039210-51039229 Msa0686530:CDS 35.0%
TGAGAAAATTCTTGGCTTCT+AGG - chr4_4:51039256-51039275 Msa0686530:CDS 35.0%
! AAAATTCTTGGCTTCTAGGA+AGG - chr4_4:51039260-51039279 Msa0686530:CDS 35.0%
TCAAATTCTCCAAAGCTTTG+AGG + chr4_4:51039302-51039321 None:intergenic 35.0%
!! CAAAGCTTTGGAGAATTTGA+AGG - chr4_4:51039302-51039321 Msa0686530:CDS 35.0%
TTGAAGGCTAATGTGAAGAA+AGG - chr4_4:51039318-51039337 Msa0686530:CDS 35.0%
GCTAATGTGAAGAAAGGTAA+AGG - chr4_4:51039324-51039343 Msa0686530:CDS 35.0%
TCATGATCCTTATTGTTGCT+AGG + chr4_4:51039363-51039382 None:intergenic 35.0%
GGATCATGAAAAAGTGACTT+TGG - chr4_4:51039374-51039393 Msa0686530:CDS 35.0%
AGTGATCACTCTTTCAATGT+TGG - chr4_4:51039419-51039438 Msa0686530:CDS 35.0%
ACCAAGTAATTATTGGTCCT+TGG - chr4_4:51039482-51039501 Msa0686530:CDS 35.0%
ACCAAGGACCAATAATTACT+TGG + chr4_4:51039486-51039505 None:intergenic 35.0%
!! CATCATAAGCTTTGAAACCA+AGG + chr4_4:51039502-51039521 None:intergenic 35.0%
CAATCATTTGCAGAACCAAT+TGG - chr4_4:51039632-51039651 Msa0686530:CDS 35.0%
GCAGAACCAATTGGAAAATT+TGG - chr4_4:51039641-51039660 Msa0686530:CDS 35.0%
! CAGACTCCAAATTTTCCAAT+TGG + chr4_4:51039650-51039669 None:intergenic 35.0%
! AATTTGGAGTCTGTTGTTCA+AGG - chr4_4:51039657-51039676 Msa0686530:CDS 35.0%
! ATTTGGAGTCTGTTGTTCAA+GGG - chr4_4:51039658-51039677 Msa0686530:CDS 35.0%
GTGATTAAGGACATTGAGAA+GGG + chr4_4:51039736-51039755 None:intergenic 35.0%
AGTGATTAAGGACATTGAGA+AGG + chr4_4:51039737-51039756 None:intergenic 35.0%
TGATGTTGGACTAATAGGAA+TGG + chr4_4:51038866-51038885 None:intergenic 35.0%
TGATGTGATGTTGGACTAAT+AGG + chr4_4:51038871-51038890 None:intergenic 35.0%
GATTGCATTGTAGAATGAGA+GGG + chr4_4:51038930-51038949 None:intergenic 35.0%
TGATTGCATTGTAGAATGAG+AGG + chr4_4:51038931-51038950 None:intergenic 35.0%
TCTACAATGCAATCAACACT+TGG - chr4_4:51038936-51038955 Msa0686530:CDS 35.0%
ATCAACTCTTCATCGTTGTT+CGG - chr4_4:51038982-51039001 Msa0686530:CDS 35.0%
! AGCTTTTAGACGGATCTTTA+AGG - chr4_4:51039115-51039134 Msa0686530:CDS 35.0%
GGATTCTCTAATTCATACGA+AGG - chr4_4:51039164-51039183 Msa0686530:CDS 35.0%
TCGATTATGCGAAGAAAAAG+AGG - chr4_4:51039207-51039226 Msa0686530:CDS 35.0%
ATTATGCGAAGAAAAAGAGG+AGG - chr4_4:51039210-51039229 Msa0686530:CDS 35.0%
TGAGAAAATTCTTGGCTTCT+AGG - chr4_4:51039256-51039275 Msa0686530:CDS 35.0%
! AAAATTCTTGGCTTCTAGGA+AGG - chr4_4:51039260-51039279 Msa0686530:CDS 35.0%
TCAAATTCTCCAAAGCTTTG+AGG + chr4_4:51039302-51039321 None:intergenic 35.0%
!! CAAAGCTTTGGAGAATTTGA+AGG - chr4_4:51039302-51039321 Msa0686530:CDS 35.0%
TTGAAGGCTAATGTGAAGAA+AGG - chr4_4:51039318-51039337 Msa0686530:CDS 35.0%
GCTAATGTGAAGAAAGGTAA+AGG - chr4_4:51039324-51039343 Msa0686530:CDS 35.0%
TCATGATCCTTATTGTTGCT+AGG + chr4_4:51039363-51039382 None:intergenic 35.0%
GGATCATGAAAAAGTGACTT+TGG - chr4_4:51039374-51039393 Msa0686530:CDS 35.0%
AGTGATCACTCTTTCAATGT+TGG - chr4_4:51039419-51039438 Msa0686530:CDS 35.0%
ACCAAGTAATTATTGGTCCT+TGG - chr4_4:51039482-51039501 Msa0686530:CDS 35.0%
ACCAAGGACCAATAATTACT+TGG + chr4_4:51039486-51039505 None:intergenic 35.0%
!! CATCATAAGCTTTGAAACCA+AGG + chr4_4:51039502-51039521 None:intergenic 35.0%
CAATCATTTGCAGAACCAAT+TGG - chr4_4:51039632-51039651 Msa0686530:CDS 35.0%
GCAGAACCAATTGGAAAATT+TGG - chr4_4:51039641-51039660 Msa0686530:CDS 35.0%
! CAGACTCCAAATTTTCCAAT+TGG + chr4_4:51039650-51039669 None:intergenic 35.0%
! AATTTGGAGTCTGTTGTTCA+AGG - chr4_4:51039657-51039676 Msa0686530:CDS 35.0%
! ATTTGGAGTCTGTTGTTCAA+GGG - chr4_4:51039658-51039677 Msa0686530:CDS 35.0%
GTGATTAAGGACATTGAGAA+GGG + chr4_4:51039736-51039755 None:intergenic 35.0%
AGTGATTAAGGACATTGAGA+AGG + chr4_4:51039737-51039756 None:intergenic 35.0%
ACATGATTGCGTCGAAAGTT+TGG - chr4_4:51039032-51039051 Msa0686530:CDS 40.0%
!! ACAAGGACTTGTTGAGTAAG+AGG + chr4_4:51039066-51039085 None:intergenic 40.0%
AGATCCGTCTAAAAGCTCAT+CGG + chr4_4:51039112-51039131 None:intergenic 40.0%
TTGCTACAGAACACACATCT+AGG + chr4_4:51039143-51039162 None:intergenic 40.0%
AGATGTGTGTTCTGTAGCAA+AGG - chr4_4:51039143-51039162 Msa0686530:CDS 40.0%
AAGAAAAAGAGGAGGCGAAA+TGG - chr4_4:51039218-51039237 Msa0686530:CDS 40.0%
! AGCCAAGAATTTTCTCACCT+CGG + chr4_4:51039253-51039272 None:intergenic 40.0%
GAAAGCAATCCTCAAAGCTT+TGG - chr4_4:51039290-51039309 Msa0686530:CDS 40.0%
!! GGTTAGTTTGTTAAGAGACG+TGG - chr4_4:51039395-51039414 Msa0686530:CDS 40.0%
GCTTATGATGCAGAACAAGA+AGG - chr4_4:51039512-51039531 Msa0686530:CDS 40.0%
ACATGATTGCGTCGAAAGTT+TGG - chr4_4:51039032-51039051 Msa0686530:CDS 40.0%
!! ACAAGGACTTGTTGAGTAAG+AGG + chr4_4:51039066-51039085 None:intergenic 40.0%
AGATCCGTCTAAAAGCTCAT+CGG + chr4_4:51039112-51039131 None:intergenic 40.0%
TTGCTACAGAACACACATCT+AGG + chr4_4:51039143-51039162 None:intergenic 40.0%
AGATGTGTGTTCTGTAGCAA+AGG - chr4_4:51039143-51039162 Msa0686530:CDS 40.0%
AAGAAAAAGAGGAGGCGAAA+TGG - chr4_4:51039218-51039237 Msa0686530:CDS 40.0%
! AGCCAAGAATTTTCTCACCT+CGG + chr4_4:51039253-51039272 None:intergenic 40.0%
GAAAGCAATCCTCAAAGCTT+TGG - chr4_4:51039290-51039309 Msa0686530:CDS 40.0%
!! GGTTAGTTTGTTAAGAGACG+TGG - chr4_4:51039395-51039414 Msa0686530:CDS 40.0%
GCTTATGATGCAGAACAAGA+AGG - chr4_4:51039512-51039531 Msa0686530:CDS 40.0%
CATTGTAGAATGAGAGGGTG+TGG + chr4_4:51038925-51038944 None:intergenic 45.0%
! GAAGAGTTGATTGCGTCGAA+AGG + chr4_4:51038973-51038992 None:intergenic 45.0%
! TGGACCGATGAGCTTTTAGA+CGG - chr4_4:51039105-51039124 Msa0686530:CDS 45.0%
AAAAAGAGGAGGCGAAATGG+AGG - chr4_4:51039221-51039240 Msa0686530:CDS 45.0%
TTACTTGGTTTCGATTGCGC+CGG + chr4_4:51039471-51039490 None:intergenic 45.0%
GTCTGTTGTTCAAGGGTTTG+TGG - chr4_4:51039665-51039684 Msa0686530:CDS 45.0%
CATTGTAGAATGAGAGGGTG+TGG + chr4_4:51038925-51038944 None:intergenic 45.0%
! GAAGAGTTGATTGCGTCGAA+AGG + chr4_4:51038973-51038992 None:intergenic 45.0%
! TGGACCGATGAGCTTTTAGA+CGG - chr4_4:51039105-51039124 Msa0686530:CDS 45.0%
AAAAAGAGGAGGCGAAATGG+AGG - chr4_4:51039221-51039240 Msa0686530:CDS 45.0%
TTACTTGGTTTCGATTGCGC+CGG + chr4_4:51039471-51039490 None:intergenic 45.0%
GTCTGTTGTTCAAGGGTTTG+TGG - chr4_4:51039665-51039684 Msa0686530:CDS 45.0%
!! CGAGGTTGGTGATGTGATGT+TGG + chr4_4:51038880-51038899 None:intergenic 50.0%
! ATGAGAGGGTGTGGTTTCGA+TGG + chr4_4:51038916-51038935 None:intergenic 50.0%
TCCACGCACGTCAAGAGAAA+TGG - chr4_4:51039085-51039104 Msa0686530:CDS 50.0%
TCCATTTCTCTTGACGTGCG+TGG + chr4_4:51039089-51039108 None:intergenic 50.0%
GGCCGAGGTGAGAAAATTCT+TGG - chr4_4:51039248-51039267 Msa0686530:CDS 50.0%
!! CGAGGTTGGTGATGTGATGT+TGG + chr4_4:51038880-51038899 None:intergenic 50.0%
! ATGAGAGGGTGTGGTTTCGA+TGG + chr4_4:51038916-51038935 None:intergenic 50.0%
TCCACGCACGTCAAGAGAAA+TGG - chr4_4:51039085-51039104 Msa0686530:CDS 50.0%
TCCATTTCTCTTGACGTGCG+TGG + chr4_4:51039089-51039108 None:intergenic 50.0%
GGCCGAGGTGAGAAAATTCT+TGG - chr4_4:51039248-51039267 Msa0686530:CDS 50.0%
!! GCAAGCTTTTCGAGCGAGGT+TGG + chr4_4:51038894-51038913 None:intergenic 55.0%
!! GATGGCAAGCTTTTCGAGCG+AGG + chr4_4:51038898-51038917 None:intergenic 55.0%
TCTCTTGACGTGCGTGGACA+AGG + chr4_4:51039083-51039102 None:intergenic 55.0%
AGGAGGCGAAATGGAGGTGA+CGG - chr4_4:51039227-51039246 Msa0686530:CDS 55.0%
!! GCAAGCTTTTCGAGCGAGGT+TGG + chr4_4:51038894-51038913 None:intergenic 55.0%
!! GATGGCAAGCTTTTCGAGCG+AGG + chr4_4:51038898-51038917 None:intergenic 55.0%
TCTCTTGACGTGCGTGGACA+AGG + chr4_4:51039083-51039102 None:intergenic 55.0%
AGGAGGCGAAATGGAGGTGA+CGG - chr4_4:51039227-51039246 Msa0686530:CDS 55.0%
! CGAAATGGAGGTGACGGCCG+AGG - chr4_4:51039233-51039252 Msa0686530:CDS 65.0%
! CGAAATGGAGGTGACGGCCG+AGG - chr4_4:51039233-51039252 Msa0686530:CDS 65.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 51038853 51039758 51038853 ID=Msa0686530;Name=Msa0686530
chr4_4 mRNA 51038853 51039758 51038853 ID=Msa0686530-mRNA-1;Parent=Msa0686530;Name=Msa0686530-mRNA-1;_AED=0.37;_eAED=0.37;_QI=0|-1|0|1|-1|1|1|0|301
chr4_4 exon 51038853 51039758 51038853 ID=Msa0686530-mRNA-1:exon:13136;Parent=Msa0686530-mRNA-1
chr4_4 CDS 51038853 51039758 51038853 ID=Msa0686530-mRNA-1:cds;Parent=Msa0686530-mRNA-1
Gene Sequence

>Msa0686530

ATGGCTACTACCATTCCTATTAGTCCAACATCACATCACCAACCTCGCTCGAAAAGCTTGCCATCGAAACCACACCCTCTCATTCTACAATGCAATCAACACTTGGCAAATTTAGAGCCTTTCGACGCAATCAACTCTTCATCGTTGTTCGGTAAAAAACTAACCGATCTACAAGCTTTACATGATTGCGTCGAAAGTTTGGTATTGCTACCTCTTACTCAACAAGTCCTTGTCCACGCACGTCAAGAGAAATGGACCGATGAGCTTTTAGACGGATCTTTAAGGCTCCTAGATGTGTGTTCTGTAGCAAAGGATTCTCTAATTCATACGAAGGAATGTGCACGCGAACTTCAATCGATTATGCGAAGAAAAAGAGGAGGCGAAATGGAGGTGACGGCCGAGGTGAGAAAATTCTTGGCTTCTAGGAAGGTAGTAAAGAAAGCAATCCTCAAAGCTTTGGAGAATTTGAAGGCTAATGTGAAGAAAGGTAAAGGAAAACTATTATCTCCTAGCAACAATAAGGATCATGAAAAAGTGACTTTGGTTAGTTTGTTAAGAGACGTGGAAGTGATCACTCTTTCAATGTTGGAGTCACTTTTAATCTTTATATCTGGACCGGCGCAATCGAAACCAAGTAATTATTGGTCCTTGGTTTCAAAGCTTATGATGCAGAACAAGAAGGTTTCTTCTACACAAGAAGCTGATATCAACGAGTTTTCTAATGTTGATGCTGCATTGCAGTCTTTTGTGTTTAATGTCGCAAGAAAATCAGATAATGTCAATCATTTGCAGAACCAATTGGAAAATTTGGAGTCTGTTGTTCAAGGGTTTGTGGAAGCACTTGAAGCTCTTTTTAAACGCTTTATTAAGATTAGAGTTTCCCTTCTCAATGTCCTTAATCACTAG

Protein sequence

>Msa0686530

MATTIPISPTSHHQPRSKSLPSKPHPLILQCNQHLANLEPFDAINSSSLFGKKLTDLQALHDCVESLVLLPLTQQVLVHARQEKWTDELLDGSLRLLDVCSVAKDSLIHTKECARELQSIMRRKRGGEMEVTAEVRKFLASRKVVKKAILKALENLKANVKKGKGKLLSPSNNKDHEKVTLVSLLRDVEVITLSMLESLLIFISGPAQSKPSNYWSLVSKLMMQNKKVSSTQEADINEFSNVDAALQSFVFNVARKSDNVNHLQNQLENLESVVQGFVEALEALFKRFIKIRVSLLNVLNH*