AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0686860

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0686860 MtrunA17_Chr4g0037011 100.000 148 0 0 1 148 1 148 3.26e-110 309
Msa0686860 MtrunA17_Chr3g0108261 91.892 148 12 0 1 148 1 148 5.03e-103 291
Msa0686860 MtrunA17_Chr7g0275361 92.568 148 11 0 1 148 1 148 7.71e-103 290
Msa0686860 MtrunA17_Chr5g0432831 91.216 148 13 0 1 148 1 148 2.21e-102 289
Msa0686860 MtrunA17_Chr1g0154011 88.514 148 17 0 1 148 1 148 5.46e-100 283
Msa0686860 MtrunA17_Chr8g0369311 87.838 148 18 0 1 148 57 204 3.64e-98 281
Msa0686860 MtrunA17_Chr8g0378601 51.724 145 70 0 2 146 23 167 8.67e-55 169
Msa0686860 MtrunA17_Chr2g0316201 47.586 145 76 0 4 148 8 152 1.89e-46 148
Msa0686860 MtrunA17_Chr2g0316211 47.222 144 76 0 5 148 9 152 2.09e-45 145
Msa0686860 MtrunA17_Chr8g0362321 45.205 146 75 1 2 142 5 150 2.78e-44 142
Msa0686860 MtrunA17_Chr4g0060351 44.521 146 76 1 2 142 5 150 1.72e-43 140
Msa0686860 MtrunA17_Chr3g0131871 48.611 144 72 2 5 146 6 149 2.01e-40 134
Msa0686860 MtrunA17_Chr3g0139901 41.497 147 82 3 5 147 7 153 1.20e-34 118
Msa0686860 MtrunA17_Chr4g0038891 44.715 123 68 0 4 126 35 157 3.78e-34 117
Msa0686860 MtrunA17_Chr1g0199951 47.200 125 65 1 3 126 43 167 5.32e-34 117
Msa0686860 MtrunA17_Chr5g0396451 43.089 123 70 0 4 126 35 157 2.40e-32 113
Msa0686860 MtrunA17_Chr5g0403591 38.971 136 80 2 5 139 33 166 1.47e-28 103
Msa0686860 MtrunA17_Chr4g0053191 34.868 152 92 2 1 145 4 155 1.30e-27 100
Msa0686860 MtrunA17_Chr4g0031181 35.821 134 72 2 15 134 18 151 1.19e-26 98.2
Msa0686860 MtrunA17_Chr8g0348701 35.772 123 77 2 26 146 25 147 1.02e-25 96.3
Msa0686860 MtrunA17_Chr5g0401831 32.895 152 95 2 1 145 4 155 1.09e-25 95.5
Msa0686860 MtrunA17_Chr8g0387131 37.273 110 67 2 39 146 38 147 2.40e-24 92.8
Msa0686860 MtrunA17_Chr2g0328001 34.028 144 81 2 15 144 18 161 4.24e-24 91.7
Msa0686860 MtrunA17_Chr7g0254701 34.532 139 91 0 4 142 13 151 4.64e-24 94.0
Msa0686860 MtrunA17_Chr4g0059291 29.268 123 85 2 26 146 25 147 3.24e-21 84.7
Msa0686860 MtrunA17_Chr8g0372391 39.806 103 60 2 1 102 15 116 2.90e-19 79.0
Msa0686860 MtrunA17_Chr4g0068301 33.613 119 69 4 2 114 11 125 1.71e-17 77.0
Msa0686860 MtrunA17_Chr5g0404931 36.893 103 63 2 1 102 15 116 2.44e-17 73.9
Msa0686860 MtrunA17_Chr8g0376111 35.246 122 69 3 4 115 43 164 1.99e-16 74.3
Msa0686860 MtrunA17_Chr7g0233631 35.246 122 69 3 4 115 38 159 8.30e-16 72.8
Msa0686860 MtrunA17_Chr8g0345331 31.624 117 71 3 4 115 8 120 1.34e-15 71.2
Msa0686860 MtrunA17_Chr7g0233611 35.246 122 69 3 4 115 38 159 1.96e-15 71.6
Msa0686860 MtrunA17_Chr7g0222941 30.894 123 75 3 3 115 90 212 1.14e-14 68.6
Msa0686860 MtrunA17_Chr4g0047301 30.000 150 92 4 4 143 672 818 2.92e-14 68.9
Msa0686860 MtrunA17_Chr2g0282211 27.815 151 96 4 3 143 681 828 2.72e-13 66.2
Msa0686860 MtrunA17_Chr4g0045101 31.967 122 73 3 4 115 37 158 6.00e-13 64.7
Msa0686860 MtrunA17_Chr2g0317631 30.894 123 75 3 3 115 306 428 1.30e-12 63.9
Msa0686860 MtrunA17_Chr1g0186291 28.571 98 56 2 43 139 30 114 2.45e-12 60.5
Msa0686860 MtrunA17_Chr1g0198021 29.268 123 77 3 3 115 137 259 2.63e-12 63.2
Msa0686860 MtrunA17_Chr8g0376201 31.707 123 74 3 3 115 192 314 8.46e-12 61.6
Msa0686860 MtrunA17_Chr1g0211131 30.328 122 75 3 4 115 823 944 4.01e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0686860 AT1G64230.2 91.892 148 12 0 1 148 1 148 1.92e-103 292
Msa0686860 AT1G64230.1 91.892 148 12 0 1 148 1 148 1.92e-103 292
Msa0686860 AT5G53300.2 91.892 148 12 0 1 148 1 148 1.46e-102 290
Msa0686860 AT5G53300.4 91.892 148 12 0 1 148 1 148 1.46e-102 290
Msa0686860 AT5G53300.1 91.892 148 12 0 1 148 1 148 1.46e-102 290
Msa0686860 AT4G27960.1 91.216 148 13 0 1 148 1 148 3.48e-102 289
Msa0686860 AT5G41700.5 90.541 148 14 0 1 148 1 148 7.76e-102 288
Msa0686860 AT5G41700.2 90.541 148 14 0 1 148 1 148 7.76e-102 288
Msa0686860 AT5G41700.1 90.541 148 14 0 1 148 1 148 7.76e-102 288
Msa0686860 AT4G27960.2 91.216 148 13 0 1 148 31 178 2.30e-101 288
Msa0686860 AT3G08690.2 88.514 148 17 0 1 148 1 148 8.50e-100 283
Msa0686860 AT3G08690.1 88.514 148 17 0 1 148 1 148 8.50e-100 283
Msa0686860 AT5G56150.1 88.514 148 17 0 1 148 1 148 4.87e-99 281
Msa0686860 AT5G41700.4 89.262 149 15 1 1 148 1 149 6.21e-99 281
Msa0686860 AT1G64230.3 85.987 157 13 1 1 148 1 157 1.05e-98 281
Msa0686860 AT2G16740.1 85.714 147 21 0 1 147 1 147 3.36e-97 276
Msa0686860 AT1G64230.5 71.053 190 13 1 1 148 1 190 6.21e-94 270
Msa0686860 AT1G64230.4 71.053 190 13 1 1 148 1 190 6.21e-94 270
Msa0686860 AT3G08700.2 75.839 149 35 1 1 148 1 149 5.47e-84 243
Msa0686860 AT3G08700.1 75.839 149 35 1 1 148 1 149 5.47e-84 243
Msa0686860 AT5G41700.3 91.346 104 9 0 1 104 1 104 2.03e-69 206
Msa0686860 AT5G53300.3 89.908 109 9 1 1 107 1 109 2.19e-69 205
Msa0686860 AT5G56150.2 87.379 103 13 0 1 103 1 103 1.06e-65 196
Msa0686860 AT3G13550.1 48.966 145 74 0 2 146 37 181 1.51e-53 167
Msa0686860 AT1G16890.2 48.276 145 75 0 4 148 8 152 5.60e-47 149
Msa0686860 AT1G78870.2 47.586 145 76 0 4 148 8 152 3.70e-46 147
Msa0686860 AT5G62540.1 48.872 133 68 0 2 134 5 137 1.98e-44 143
Msa0686860 AT1G16890.3 46.104 154 74 1 4 148 8 161 2.98e-44 143
Msa0686860 AT2G02760.1 44.521 146 76 1 2 142 5 150 1.69e-43 140
Msa0686860 AT2G02760.2 44.521 146 76 1 2 142 5 150 1.69e-43 140
Msa0686860 AT2G02760.4 44.521 146 76 1 2 142 5 150 1.69e-43 140
Msa0686860 AT2G02760.3 44.521 146 76 1 2 142 5 150 1.69e-43 140
Msa0686860 AT1G14400.1 44.521 146 76 1 2 142 5 150 1.81e-43 140
Msa0686860 AT1G14400.2 44.521 146 76 1 2 142 5 150 1.81e-43 140
Msa0686860 AT3G13550.2 51.754 114 55 0 2 115 37 150 1.94e-43 141
Msa0686860 AT1G36340.2 46.897 145 76 1 2 146 9 152 4.65e-43 140
Msa0686860 AT1G36340.1 46.897 145 76 1 2 146 9 152 4.65e-43 140
Msa0686860 AT1G16890.1 50.420 119 59 0 30 148 1 119 5.99e-40 130
Msa0686860 AT1G78870.4 50.420 119 59 0 30 148 1 119 1.01e-39 130
Msa0686860 AT5G50870.1 47.917 144 73 2 5 146 6 149 2.10e-38 129
Msa0686860 AT1G78870.1 50.000 120 59 1 30 148 1 120 5.99e-38 125
Msa0686860 AT5G50870.2 44.516 155 73 3 5 146 6 160 1.87e-35 122
Msa0686860 AT3G24515.1 43.949 157 75 3 3 146 8 164 2.05e-33 121
Msa0686860 AT5G25760.1 40.136 147 84 3 5 147 7 153 4.42e-33 114
Msa0686860 AT5G25760.2 40.136 147 84 3 5 147 7 153 4.42e-33 114
Msa0686860 AT2G32790.1 51.587 126 57 2 25 148 54 177 9.54e-33 114
Msa0686860 AT1G78870.3 48.571 105 54 0 4 108 8 112 2.85e-32 111
Msa0686860 AT3G20060.1 43.089 123 70 0 4 126 39 161 7.90e-31 109
Msa0686860 AT3G24515.3 45.902 122 62 1 29 146 68 189 1.77e-30 114
Msa0686860 AT1G50490.1 43.902 123 69 0 4 126 38 160 2.34e-30 108
Msa0686860 AT3G20060.2 45.714 105 57 0 22 126 12 116 4.00e-29 103
Msa0686860 AT3G57870.1 35.526 152 91 3 1 145 5 156 1.90e-27 100
Msa0686860 AT4G36800.2 38.235 136 81 2 5 139 34 167 3.23e-27 100
Msa0686860 AT4G36800.1 38.235 136 81 2 5 139 34 167 3.23e-27 100
Msa0686860 AT2G46030.5 35.135 148 93 3 1 146 1 147 3.78e-27 100
Msa0686860 AT2G46030.1 35.135 148 93 3 1 146 1 147 3.78e-27 100
Msa0686860 AT5G05080.2 37.410 139 87 0 4 142 13 151 5.91e-27 101
Msa0686860 AT5G05080.3 37.410 139 87 0 4 142 13 151 5.91e-27 101
Msa0686860 AT5G05080.1 37.410 139 87 0 4 142 13 151 5.91e-27 101
Msa0686860 AT5G05080.4 37.410 139 87 0 4 142 13 151 5.91e-27 101
Msa0686860 AT2G46030.3 35.135 148 93 3 1 146 73 219 1.01e-26 100
Msa0686860 AT2G46030.4 38.211 123 74 2 26 146 28 150 1.03e-26 99.4
Msa0686860 AT2G46030.2 38.211 123 74 2 26 146 28 150 1.03e-26 99.4
Msa0686860 AT5G41340.1 36.585 123 76 2 26 146 25 147 7.01e-26 97.1
Msa0686860 AT1G63800.2 36.585 123 76 2 26 146 10 132 8.79e-26 96.3
Msa0686860 AT1G63800.1 36.585 123 76 2 26 146 25 147 9.53e-26 96.7
Msa0686860 AT3G55380.1 35.075 134 73 2 15 134 19 152 2.64e-25 95.1
Msa0686860 AT5G59300.1 36.296 135 72 2 15 135 18 152 1.09e-24 93.6
Msa0686860 AT3G46460.1 34.028 144 81 2 15 144 18 161 1.63e-24 93.2
Msa0686860 AT2G18600.1 36.496 137 82 4 5 139 35 168 3.13e-24 92.8
Msa0686860 AT2G18600.2 36.496 137 82 4 5 139 35 168 3.13e-24 92.8
Msa0686860 AT5G41340.2 38.462 104 62 2 45 146 9 112 1.02e-22 88.2
Msa0686860 AT3G17000.1 36.134 119 66 4 2 114 12 126 3.23e-19 82.0
Msa0686860 AT1G75440.1 38.835 103 61 2 1 102 15 116 6.27e-19 78.6
Msa0686860 AT3G55380.2 35.965 114 57 3 15 112 19 132 3.40e-18 77.4
Msa0686860 AT1G45050.1 37.864 103 62 2 1 102 15 116 4.93e-18 76.3
Msa0686860 AT5G42990.1 38.835 103 61 2 1 102 15 116 9.98e-18 75.5
Msa0686860 AT1G17280.9 33.333 117 69 3 4 115 8 120 1.03e-16 74.3
Msa0686860 AT1G17280.7 33.333 117 69 3 4 115 8 120 1.03e-16 74.3
Msa0686860 AT1G17280.6 33.333 117 69 3 4 115 8 120 1.03e-16 74.3
Msa0686860 AT1G17280.2 33.333 117 69 3 4 115 8 120 1.03e-16 74.3
Msa0686860 AT1G17280.5 33.333 117 69 3 4 115 8 120 1.03e-16 74.3
Msa0686860 AT1G17280.3 33.333 117 69 3 4 115 8 120 1.03e-16 74.3
Msa0686860 AT1G17280.1 33.333 117 69 3 4 115 8 120 1.03e-16 74.3
Msa0686860 AT1G17280.8 33.333 117 69 3 4 115 8 120 1.03e-16 74.3
Msa0686860 AT4G36410.1 35.185 108 64 3 1 107 15 117 1.34e-16 72.4
Msa0686860 AT5G50430.1 33.333 117 69 3 4 115 8 120 1.35e-16 74.3
Msa0686860 AT5G50430.2 33.333 117 69 3 4 115 8 120 1.35e-16 74.3
Msa0686860 AT3G46460.3 30.631 111 64 1 47 144 9 119 4.35e-16 70.1
Msa0686860 AT3G46460.2 30.631 111 64 1 47 144 9 119 4.35e-16 70.1
Msa0686860 AT1G17280.4 30.894 123 70 3 4 115 8 126 1.96e-15 70.9
Msa0686860 AT3G15355.1 29.139 151 94 4 3 143 334 481 7.24e-12 62.4
Msa0686860 AT1G53025.2 31.148 122 74 3 4 115 274 395 2.06e-11 60.8
Msa0686860 AT1G45050.2 34.831 89 56 2 1 88 15 102 2.09e-11 57.8
Msa0686860 AT1G53025.1 31.148 122 74 3 4 115 274 395 2.15e-11 60.8
Msa0686860 AT1G53023.1 31.148 122 74 3 4 115 60 181 9.35e-11 58.9

Find 113 sgRNAs with CRISPR-Local

Find 188 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTAATTATGGATCTAATT+TGG 0.107513 4_4:-51468998 Msa0686860:three_prime_UTR
AAAGACGCGGCCTTGAAATT+TGG 0.144554 4_4:-51469937 Msa0686860:three_prime_UTR
ATCTTGTGTTTCGTGCTTTA+TGG 0.171568 4_4:-51469226 Msa0686860:three_prime_UTR
AGATTTACTGATGTTAATTA+TGG 0.182112 4_4:-51469009 Msa0686860:three_prime_UTR
TGCACAAATTTATCCCTTCT+TGG 0.235806 4_4:+51469789 None:intergenic
GAATAAATTCTACTCTCTTT+TGG 0.250551 4_4:-51469845 Msa0686860:three_prime_UTR
CAGGTTGCAGGCATATTTAT+TGG 0.271991 4_4:+51471316 None:intergenic
ACTCTTGTTTAGTGTTTCAT+TGG 0.289354 4_4:-51469189 Msa0686860:three_prime_UTR
GTTAATTATGGATCTAATTT+GGG 0.290297 4_4:-51468997 Msa0686860:three_prime_UTR
TAATGGTTGGATCGATTATA+TGG 0.292507 4_4:+51469386 None:intergenic
CCACAAGTGTGTAGCAAATA+TGG 0.302149 4_4:+51469043 None:intergenic
AAGACGCGGCCTTGAAATTT+GGG 0.308012 4_4:-51469936 Msa0686860:three_prime_UTR
TGTGCAGAAAATAATAAATA+TGG 0.317449 4_4:+51469142 None:intergenic
AACATAACATTAGCAAAATA+TGG 0.320168 4_4:+51468958 None:intergenic
ATTCTGATTGCCATTGACTA+TGG 0.323541 4_4:-51469658 Msa0686860:three_prime_UTR
ATAGGAACATGTGGCCTAAT+TGG 0.333623 4_4:+51469482 None:intergenic
AGAAGAGAACCGCTTGAATA+TGG 0.338115 4_4:-51471251 Msa0686860:exon
CATTTAGAACCCCTAAAATC+TGG 0.350986 4_4:-51469691 Msa0686860:three_prime_UTR
CACATAACATTTGCAAAATT+AGG 0.365332 4_4:+51468899 None:intergenic
GAGAATAGTTAAGGAGCTTA+AGG 0.366328 4_4:-51471221 Msa0686860:CDS
GGAGTTAGTCTTACAGTTGA+TGG 0.368318 4_4:+51469503 None:intergenic
AGATAAGATTACAAGAATAA+AGG 0.373866 4_4:+51471449 None:intergenic
GGCCTAAAAGTAGCTGTTAA+TGG 0.376008 4_4:+51469369 None:intergenic
CATTGGCAAGCAACTATCAT+TGG 0.378999 4_4:-51471061 Msa0686860:CDS
CTGTAAGACTAACTCCAATT+AGG 0.395549 4_4:-51469496 Msa0686860:three_prime_UTR
GGAGGTTTGAAGGGATAATC+AGG 0.402929 4_4:+51470983 None:intergenic
ATAAATTTGTGCACTTCCTC+AGG 0.402934 4_4:-51469779 Msa0686860:three_prime_UTR
ACCTTGGATATTGTGAGAGC+AGG 0.413179 4_4:+51470246 None:intergenic
GTTAGGGAGGAGTCAAGTTA+AGG 0.423935 4_4:+51469741 None:intergenic
ACAAGAGGGTCATCAGGATT+TGG 0.424690 4_4:+51470096 None:intergenic
ACAGTTGATGGTGGTGATAA+TGG 0.425707 4_4:+51469515 None:intergenic
ATCCGTTAGCAGAGAACATA+TGG 0.433316 4_4:+51470119 None:intergenic
CCAGCATAGGGACTATCATT+TGG 0.439200 4_4:+51471037 None:intergenic
AAGCGGTTCTCTTCTCAGTA+AGG 0.442442 4_4:+51471259 None:intergenic
CAAGAAATAAAACAGGTTGC+AGG 0.442953 4_4:+51471304 None:intergenic
TCAGCACTGCCCAAATTTCA+AGG 0.443228 4_4:+51469927 None:intergenic
AAGCTCCTTAACTATTCTCT+TGG 0.446675 4_4:+51471225 None:intergenic
GTATACCTTGGGAGGTTTGA+AGG 0.451790 4_4:+51470973 None:intergenic
GGCAAAAGATGAGCATATCT+TGG 0.460613 4_4:+51469345 None:intergenic
AGCATATATTCCTCAAGAAA+AGG 0.462112 4_4:+51471425 None:intergenic
ATATTTATTGGAGCATATAG+TGG 0.466226 4_4:+51471328 None:intergenic
ATTGTTCTTTGAGTATGTCT+AGG 0.473064 4_4:+51470274 None:intergenic
TTGGAGCATATAGTGGGTGT+TGG 0.475454 4_4:+51471335 None:intergenic
TCAGGCACAAGAGGGTCATC+AGG 0.475511 4_4:+51470090 None:intergenic
GTTCTCTTCTCAGTAAGGAT+TGG 0.482658 4_4:+51471264 None:intergenic
TTGCACATATGAGCAACTTC+AGG 0.491907 4_4:+51470072 None:intergenic
TCGGTGAGGACATGTTTCAT+TGG 0.492631 4_4:-51471078 Msa0686860:CDS
CTCCATATGTTCTCTGCTAA+CGG 0.494453 4_4:-51470121 Msa0686860:CDS
TTGCCATTGCTGTTAATGTT+GGG 0.496480 4_4:+51470303 None:intergenic
CCTGCACTGCATGAGGTTGG+TGG 0.496861 4_4:+51471184 None:intergenic
TCATTGTTAACCATAGTCAA+TGG 0.497956 4_4:+51469648 None:intergenic
CCACCAACCTCATGCAGTGC+AGG 0.500851 4_4:-51471184 Msa0686860:intron
CTGGACTCAGAAATTTGCTA+TGG 0.506602 4_4:-51470013 Msa0686860:CDS
TGAAACATGTCCTCACCGAC+TGG 0.508985 4_4:+51471082 None:intergenic
CAAACTACAAGAAATAAAAC+AGG 0.510875 4_4:+51471297 None:intergenic
AATGTCCATCCTCTGCAAAC+CGG 0.514112 4_4:-51469306 Msa0686860:three_prime_UTR
AAGGAAAACACCACCAGCAT+AGG 0.515996 4_4:+51471024 None:intergenic
ATTGCCATTGCTGTTAATGT+TGG 0.518283 4_4:+51470302 None:intergenic
AGACATAATGAAGAACTAGA+AGG 0.518515 4_4:+51469282 None:intergenic
ATGAGTCCACTGCTAGAAGC+TGG 0.523736 4_4:-51470032 Msa0686860:CDS
TTACCTGCACTGCATGAGGT+TGG 0.530132 4_4:+51471181 None:intergenic
GGCGTCCAAGAGAATAGTTA+AGG 0.531160 4_4:-51471230 Msa0686860:CDS
TAAAAGTAGCTGTTAATGGT+TGG 0.531639 4_4:+51469373 None:intergenic
ATAGTCAATGGCAATCAGAA+TGG 0.541362 4_4:+51469660 None:intergenic
TATACCTTGGGAGGTTTGAA+GGG 0.541616 4_4:+51470974 None:intergenic
TATTTATTGGAGCATATAGT+GGG 0.545894 4_4:+51471329 None:intergenic
AAGGACCGGTTTGCAGAGGA+TGG 0.546171 4_4:+51469301 None:intergenic
TGACTATGGTTAACAATGAA+TGG 0.546556 4_4:-51469644 Msa0686860:three_prime_UTR
TATGCTGTACCCACCTCCAT+TGG 0.547984 4_4:-51471408 Msa0686860:five_prime_UTR
CTCTTTACCTGCACTGCATG+AGG 0.548184 4_4:+51471177 None:intergenic
CCATATTTGCTACACACTTG+TGG 0.553112 4_4:-51469043 Msa0686860:three_prime_UTR
AATTATATCTTAGTTGAAAG+CGG 0.554861 4_4:-51471500 Msa0686860:five_prime_UTR
ACACATGGGTTTGCTAGTTG+TGG 0.564717 4_4:-51469122 Msa0686860:three_prime_UTR
CACGAAACACAAGATATATG+TGG 0.565358 4_4:+51469234 None:intergenic
TAATGAAGAACTAGAAGGAC+CGG 0.570940 4_4:+51469287 None:intergenic
CCTTGGATATTGTGAGAGCA+GGG 0.575241 4_4:+51470247 None:intergenic
CTGAGTCCAGCTTCTAGCAG+TGG 0.582567 4_4:+51470026 None:intergenic
AAAAGATGAGCATATCTTGG+AGG 0.585127 4_4:+51469348 None:intergenic
AGGAAAACACCACCAGCATA+GGG 0.585785 4_4:+51471025 None:intergenic
CTACTCATCGAATTACAGAA+TGG 0.587340 4_4:-51469261 Msa0686860:three_prime_UTR
TACAATAGTAACTTAAGTCT+AGG 0.589560 4_4:+51469165 None:intergenic
CCAAATGATAGTCCCTATGC+TGG 0.590321 4_4:-51471037 Msa0686860:CDS
ACTTTGGTGTATACCTTGGG+AGG 0.590340 4_4:+51470965 None:intergenic
TTATCCCTTCAAACCTCCCA+AGG 0.591046 4_4:-51470978 Msa0686860:intron
ATAATCAGGAGGAAAATGAA+TGG 0.593685 4_4:+51470997 None:intergenic
CATTGCTGTTAATGTTGGGA+TGG 0.595351 4_4:+51470307 None:intergenic
TGGACTCAGAAATTTGCTAT+GGG 0.595611 4_4:-51470012 Msa0686860:CDS
CCCTGCTCTCACAATATCCA+AGG 0.597960 4_4:-51470247 Msa0686860:intron
TGAGCAACTTCAGGCACAAG+AGG 0.601737 4_4:+51470081 None:intergenic
CATCCCAACATTAACAGCAA+TGG 0.604832 4_4:-51470306 Msa0686860:CDS
TGAATGGTGGTGACAAAAGA+AGG 0.606788 4_4:-51469628 Msa0686860:three_prime_UTR
GCTTCAAATTTGTTGACCTG+AGG 0.612617 4_4:+51469763 None:intergenic
TGATGAGTTTCAGGTCCAGT+CGG 0.615892 4_4:-51471097 Msa0686860:intron
TGTTGGAGAGAGACAATGAA+AGG 0.621684 4_4:+51471352 None:intergenic
GCAGGTTGCATTCAGGACCA+AGG 0.630304 4_4:-51470334 Msa0686860:intron
TAGACATACTCAAAGAACAA+TGG 0.637759 4_4:-51470272 Msa0686860:CDS
TCTTGGACGCCATATTCAAG+CGG 0.638332 4_4:+51471242 None:intergenic
CTAGAAGGACCGGTTTGCAG+AGG 0.651323 4_4:+51469297 None:intergenic
GAGCAACTTCAGGCACAAGA+GGG 0.655538 4_4:+51470082 None:intergenic
AAAGTATTGAAGGAGACAAG+AGG 0.657206 4_4:+51469975 None:intergenic
GAGGAAAATGAATGGTGACA+AGG 0.659276 4_4:+51471005 None:intergenic
AGTTTCAGGTCCAGTCGGTG+AGG 0.671300 4_4:-51471092 Msa0686860:intron
AATGTTGGGATGGAATACCT+TGG 0.674072 4_4:+51470317 None:intergenic
AAGCATAAAGAATAAGACCA+TGG 0.678522 4_4:-51468857 Msa0686860:three_prime_UTR
CTCATCGAATTACAGAATGG+AGG 0.679157 4_4:-51469258 Msa0686860:three_prime_UTR
GATGGTGGTGATAATGGTGA+AGG 0.680477 4_4:+51469521 None:intergenic
GTTAGTCTTACAGTTGATGG+TGG 0.681350 4_4:+51469506 None:intergenic
AATGATAGTCCCTATGCTGG+TGG 0.684815 4_4:-51471034 Msa0686860:CDS
TAGTAGACTTAAATATACCA+TGG 0.691861 4_4:+51468840 None:intergenic
GGTTTGAAGGGATAATCAGG+AGG 0.711960 4_4:+51470986 None:intergenic
ATGGTGGTGACAAAAGAAGG+TGG 0.718551 4_4:-51469625 Msa0686860:three_prime_UTR
TGTTTGAATTGTAAAAGACG+CGG 0.719191 4_4:-51469950 Msa0686860:three_prime_UTR
CTATGGTTAACAATGAATGG+TGG 0.764283 4_4:-51469641 Msa0686860:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTTAAAAAAAAAAAAAAAAC+AGG + chr4_4:51469715-51469734 None:intergenic 10.0%
!! AAAAAAATAAACCTATTATA+TGG + chr4_4:51469769-51469788 None:intergenic 10.0%
!!! TATGATTTGTTATGTATAAT+AGG - chr4_4:51469522-51469541 Msa0686860:three_prime_UTR 15.0%
!!! ATGATTTGTTATGTATAATA+GGG - chr4_4:51469523-51469542 Msa0686860:three_prime_UTR 15.0%
!!! AATATCAAGATTTTAAATCT+TGG + chr4_4:51469931-51469950 None:intergenic 15.0%
!!! TTCAGCATTTTTATATAAAA+CGG + chr4_4:51470144-51470163 None:intergenic 15.0%
!! AATTATATCTTAGTTGAAAG+CGG - chr4_4:51468820-51468839 Msa0686860:three_prime_UTR 20.0%
!! AGATAAGATTACAAGAATAA+AGG + chr4_4:51468874-51468893 None:intergenic 20.0%
!! AAGACAAACATATCTATTTA+AGG + chr4_4:51469487-51469506 None:intergenic 20.0%
!!! AGTATAAAAAATGATTTTGC+AGG - chr4_4:51469968-51469987 Msa0686860:three_prime_UTR 20.0%
!!! TTCAAACAAAAAAGTATTGA+AGG + chr4_4:51470358-51470377 None:intergenic 20.0%
!! TATTAAAAATGTACCAAGAA+GGG - chr4_4:51470518-51470537 Msa0686860:intron 20.0%
!! TGTGCAGAAAATAATAAATA+TGG + chr4_4:51471181-51471200 None:intergenic 20.0%
!! AGATTTACTGATGTTAATTA+TGG - chr4_4:51471311-51471330 Msa0686860:five_prime_UTR 20.0%
!! TGTTAATTATGGATCTAATT+TGG - chr4_4:51471322-51471341 Msa0686860:five_prime_UTR 20.0%
!! GTTAATTATGGATCTAATTT+GGG - chr4_4:51471323-51471342 Msa0686860:five_prime_UTR 20.0%
!! AACATAACATTAGCAAAATA+TGG + chr4_4:51471365-51471384 None:intergenic 20.0%
!!! CATTTGTTGTTTTTTACCAA+TGG + chr4_4:51468931-51468950 None:intergenic 25.0%
! CATTGGTAAAAAACAACAAA+TGG - chr4_4:51468929-51468948 Msa0686860:three_prime_UTR 25.0%
! TATTTATTGGAGCATATAGT+GGG + chr4_4:51468994-51469013 None:intergenic 25.0%
! ATATTTATTGGAGCATATAG+TGG + chr4_4:51468995-51469014 None:intergenic 25.0%
! CAAACTACAAGAAATAAAAC+AGG + chr4_4:51469026-51469045 None:intergenic 25.0%
!!! TGTTTTTTATGATGAGTTTC+AGG - chr4_4:51469214-51469233 Msa0686860:three_prime_UTR 25.0%
! CTGGAATAAATAAAGAAAAC+AGG + chr4_4:51469401-51469420 None:intergenic 25.0%
! AATAAACCTATTATATGGAC+TGG + chr4_4:51469764-51469783 None:intergenic 25.0%
! AAAAAGATCTGTTGTGATTT+AGG - chr4_4:51469792-51469811 Msa0686860:three_prime_UTR 25.0%
! GTTAAAAATCATGATGATAC+AGG - chr4_4:51470169-51470188 Msa0686860:intron 25.0%
!! GAATAAATTCTACTCTCTTT+TGG - chr4_4:51470475-51470494 Msa0686860:intron 25.0%
!! CTTCTTGGTACATTTTTAAT+AGG + chr4_4:51470519-51470538 None:intergenic 25.0%
! CTATTAAAAATGTACCAAGA+AGG - chr4_4:51470517-51470536 Msa0686860:intron 25.0%
! AGCACTAAAAACATGTATTT+GGG + chr4_4:51470757-51470776 None:intergenic 25.0%
! TAGCACTAAAAACATGTATT+TGG + chr4_4:51470758-51470777 None:intergenic 25.0%
! TACAATAGTAACTTAAGTCT+AGG + chr4_4:51471158-51471177 None:intergenic 25.0%
!! TTTATTATTTTCTGCACACA+TGG - chr4_4:51471183-51471202 Msa0686860:intron 25.0%
!! TTATTATTTTCTGCACACAT+GGG - chr4_4:51471184-51471203 Msa0686860:intron 25.0%
!! TGTTTATTGTTGTGTTTTGT+TGG - chr4_4:51471234-51471253 Msa0686860:CDS 25.0%
! CACATAACATTTGCAAAATT+AGG + chr4_4:51471424-51471443 None:intergenic 25.0%
! TAGTAGACTTAAATATACCA+TGG + chr4_4:51471483-51471502 None:intergenic 25.0%
! ATCTTATCTTCCTTTTCTTG+AGG - chr4_4:51468885-51468904 Msa0686860:three_prime_UTR 30.0%
AGCATATATTCCTCAAGAAA+AGG + chr4_4:51468898-51468917 None:intergenic 30.0%
!!! TTGTTGTTTTTTACCAATGG+AGG + chr4_4:51468928-51468947 None:intergenic 30.0%
AGTTTGAATGAAACTTGAAC+TGG - chr4_4:51469164-51469183 Msa0686860:three_prime_UTR 30.0%
ATAATCAGGAGGAAAATGAA+TGG + chr4_4:51469326-51469345 None:intergenic 30.0%
!!! ACAGTGTTTTAAAAGCACTT+TGG + chr4_4:51469374-51469393 None:intergenic 30.0%
GTTAATAAGTGACTCTAGAT+AGG - chr4_4:51469430-51469449 Msa0686860:intron 30.0%
! GTATAATAGGGATTTGAGTT+TGG - chr4_4:51469535-51469554 Msa0686860:three_prime_UTR 30.0%
ACCATCTAAACTCACAATAA+CGG - chr4_4:51469577-51469596 Msa0686860:three_prime_UTR 30.0%
TGCAACTATCTGTAATATCA+AGG - chr4_4:51469890-51469909 Msa0686860:three_prime_UTR 30.0%
ATTGTTCTTTGAGTATGTCT+AGG + chr4_4:51470049-51470068 None:intergenic 30.0%
TAGACATACTCAAAGAACAA+TGG - chr4_4:51470048-51470067 Msa0686860:CDS 30.0%
TGTTTGAATTGTAAAAGACG+CGG - chr4_4:51470370-51470389 Msa0686860:intron 30.0%
! TGTGTCTTTTGCTTTAGTTA+GGG + chr4_4:51470598-51470617 None:intergenic 30.0%
! TTGTGTCTTTTGCTTTAGTT+AGG + chr4_4:51470599-51470618 None:intergenic 30.0%
TCATTGTTAACCATAGTCAA+TGG + chr4_4:51470675-51470694 None:intergenic 30.0%
TGACTATGGTTAACAATGAA+TGG - chr4_4:51470676-51470695 Msa0686860:intron 30.0%
ACTAAAAACATGTATTTGGG+AGG + chr4_4:51470754-51470773 None:intergenic 30.0%
TAATGGTTGGATCGATTATA+TGG + chr4_4:51470937-51470956 None:intergenic 30.0%
TAAAAGTAGCTGTTAATGGT+TGG + chr4_4:51470950-51470969 None:intergenic 30.0%
!!! AACCATTAACAGCTACTTTT+AGG - chr4_4:51470949-51470968 Msa0686860:intron 30.0%
! ATCTTTTGCCTCAAAAAAGA+AGG - chr4_4:51470988-51471007 Msa0686860:CDS 30.0%
!!! ACATTTCTCCTTCTTTTTTG+AGG + chr4_4:51470999-51471018 None:intergenic 30.0%
AGACATAATGAAGAACTAGA+AGG + chr4_4:51471041-51471060 None:intergenic 30.0%
! ACTCTTGTTTAGTGTTTCAT+TGG - chr4_4:51471131-51471150 Msa0686860:intron 30.0%
AAGCATAAAGAATAAGACCA+TGG - chr4_4:51471463-51471482 Msa0686860:five_prime_UTR 30.0%
!!! TGTTTTTTACCAATGGAGGT+GGG + chr4_4:51468924-51468943 None:intergenic 35.0%
!!! TTGTTTTTTACCAATGGAGG+TGG + chr4_4:51468925-51468944 None:intergenic 35.0%
CAAGAAATAAAACAGGTTGC+AGG + chr4_4:51469019-51469038 None:intergenic 35.0%
AAGCTCCTTAACTATTCTCT+TGG + chr4_4:51469098-51469117 None:intergenic 35.0%
GAGAATAGTTAAGGAGCTTA+AGG - chr4_4:51469099-51469118 Msa0686860:three_prime_UTR 35.0%
ACTTATTAACAAGATGCAGC+TGG + chr4_4:51469420-51469439 None:intergenic 35.0%
TAAGGCTATCACACACAAAA+AGG + chr4_4:51469469-51469488 None:intergenic 35.0%
GTTATTGTGAGTTTAGATGG+TGG + chr4_4:51469578-51469597 None:intergenic 35.0%
ACCGTTATTGTGAGTTTAGA+TGG + chr4_4:51469581-51469600 None:intergenic 35.0%
! TCAGTCGTTAATTTGAGATC+GGG + chr4_4:51469648-51469667 None:intergenic 35.0%
! CTCAGTCGTTAATTTGAGAT+CGG + chr4_4:51469649-51469668 None:intergenic 35.0%
!! AAGGTTTGCTGTTGCAATTT+AGG - chr4_4:51469738-51469757 Msa0686860:three_prime_UTR 35.0%
TTTAGGCCAGTCCATATAAT+AGG - chr4_4:51469755-51469774 Msa0686860:three_prime_UTR 35.0%
AGTTACGGTTACAGATACAT+CGG + chr4_4:51469835-51469854 None:intergenic 35.0%
! TTGCCATTGCTGTTAATGTT+GGG + chr4_4:51470020-51470039 None:intergenic 35.0%
! ATTGCCATTGCTGTTAATGT+TGG + chr4_4:51470021-51470040 None:intergenic 35.0%
!! TGGACTCAGAAATTTGCTAT+GGG - chr4_4:51470308-51470327 Msa0686860:CDS 35.0%
!! AAAGTATTGAAGGAGACAAG+AGG + chr4_4:51470348-51470367 None:intergenic 35.0%
TGCACAAATTTATCCCTTCT+TGG + chr4_4:51470534-51470553 None:intergenic 35.0%
ATAAATTTGTGCACTTCCTC+AGG - chr4_4:51470541-51470560 Msa0686860:intron 35.0%
! CATTTAGAACCCCTAAAATC+TGG - chr4_4:51470629-51470648 Msa0686860:intron 35.0%
! GAAATGAAGCCAGATTTTAG+GGG + chr4_4:51470641-51470660 None:intergenic 35.0%
! GGAAATGAAGCCAGATTTTA+GGG + chr4_4:51470642-51470661 None:intergenic 35.0%
! TGGAAATGAAGCCAGATTTT+AGG + chr4_4:51470643-51470662 None:intergenic 35.0%
ATAGTCAATGGCAATCAGAA+TGG + chr4_4:51470663-51470682 None:intergenic 35.0%
! ATTCTGATTGCCATTGACTA+TGG - chr4_4:51470662-51470681 Msa0686860:intron 35.0%
CTATGGTTAACAATGAATGG+TGG - chr4_4:51470679-51470698 Msa0686860:intron 35.0%
CTGTAAGACTAACTCCAATT+AGG - chr4_4:51470824-51470843 Msa0686860:intron 35.0%
AATGATTGTGTGGAAGTTTG+TGG + chr4_4:51470868-51470887 None:intergenic 35.0%
TATATGGAAATTGCTGACGA+AGG + chr4_4:51470921-51470940 None:intergenic 35.0%
AAAAGATGAGCATATCTTGG+AGG + chr4_4:51470975-51470994 None:intergenic 35.0%
TAATGAAGAACTAGAAGGAC+CGG + chr4_4:51471036-51471055 None:intergenic 35.0%
CTACTCATCGAATTACAGAA+TGG - chr4_4:51471059-51471078 Msa0686860:CDS 35.0%
CACGAAACACAAGATATATG+TGG + chr4_4:51471089-51471108 None:intergenic 35.0%
ATCTTGTGTTTCGTGCTTTA+TGG - chr4_4:51471094-51471113 Msa0686860:intron 35.0%
TGTTGGAGAGAGACAATGAA+AGG + chr4_4:51468971-51468990 None:intergenic 40.0%
CAGGTTGCAGGCATATTTAT+TGG + chr4_4:51469007-51469026 None:intergenic 40.0%
GTTCTCTTCTCAGTAAGGAT+TGG + chr4_4:51469059-51469078 None:intergenic 40.0%
AGAAGAGAACCGCTTGAATA+TGG - chr4_4:51469069-51469088 Msa0686860:three_prime_UTR 40.0%
CATTGGCAAGCAACTATCAT+TGG - chr4_4:51469259-51469278 Msa0686860:three_prime_UTR 40.0%
GAGGAAAATGAATGGTGACA+AGG + chr4_4:51469318-51469337 None:intergenic 40.0%
TATACCTTGGGAGGTTTGAA+GGG + chr4_4:51469349-51469368 None:intergenic 40.0%
! AGCACTTTGGTGTATACCTT+GGG + chr4_4:51469361-51469380 None:intergenic 40.0%
! AAGCACTTTGGTGTATACCT+TGG + chr4_4:51469362-51469381 None:intergenic 40.0%
! TAGGGATTTGAGTTTGGACA+CGG - chr4_4:51469541-51469560 Msa0686860:three_prime_UTR 40.0%
CATCGGAAATCTTGATGTCA+AGG + chr4_4:51469818-51469837 None:intergenic 40.0%
! TGATTTTGCAGGTTGCATTC+AGG - chr4_4:51469979-51469998 Msa0686860:three_prime_UTR 40.0%
AATGTTGGGATGGAATACCT+TGG + chr4_4:51470006-51470025 None:intergenic 40.0%
! CATTGCTGTTAATGTTGGGA+TGG + chr4_4:51470016-51470035 None:intergenic 40.0%
CATCCCAACATTAACAGCAA+TGG - chr4_4:51470014-51470033 Msa0686860:CDS 40.0%
GGTTCACAATAACATGACCT+TGG + chr4_4:51470093-51470112 None:intergenic 40.0%
AGAAACTAATGACGTCAACG+AGG + chr4_4:51470119-51470138 None:intergenic 40.0%
CTCCATATGTTCTCTGCTAA+CGG - chr4_4:51470199-51470218 Msa0686860:intron 40.0%
ATCCGTTAGCAGAGAACATA+TGG + chr4_4:51470204-51470223 None:intergenic 40.0%
TTGCACATATGAGCAACTTC+AGG + chr4_4:51470251-51470270 None:intergenic 40.0%
!! CTGGACTCAGAAATTTGCTA+TGG - chr4_4:51470307-51470326 Msa0686860:CDS 40.0%
!! GCTTCAAATTTGTTGACCTG+AGG + chr4_4:51470560-51470579 None:intergenic 40.0%
! GTCTTTTGCTTTAGTTAGGG+AGG + chr4_4:51470595-51470614 None:intergenic 40.0%
! TGAATGGTGGTGACAAAAGA+AGG - chr4_4:51470692-51470711 Msa0686860:intron 40.0%
!! ACAGTTGATGGTGGTGATAA+TGG + chr4_4:51470808-51470827 None:intergenic 40.0%
!! GTTAGTCTTACAGTTGATGG+TGG + chr4_4:51470817-51470836 None:intergenic 40.0%
!! GGAGTTAGTCTTACAGTTGA+TGG + chr4_4:51470820-51470839 None:intergenic 40.0%
ATAGGAACATGTGGCCTAAT+TGG + chr4_4:51470841-51470860 None:intergenic 40.0%
!! TGTGGTGTTATAGGAACATG+TGG + chr4_4:51470850-51470869 None:intergenic 40.0%
!! GTGGAAGTTTGTGGTGTTAT+AGG + chr4_4:51470859-51470878 None:intergenic 40.0%
ACTTGAGTTAAGGAATGTGC+TGG + chr4_4:51470898-51470917 None:intergenic 40.0%
GGCCTAAAAGTAGCTGTTAA+TGG + chr4_4:51470954-51470973 None:intergenic 40.0%
GGCAAAAGATGAGCATATCT+TGG + chr4_4:51470978-51470997 None:intergenic 40.0%
CTCATCGAATTACAGAATGG+AGG - chr4_4:51471062-51471081 Msa0686860:CDS 40.0%
CCACAAGTGTGTAGCAAATA+TGG + chr4_4:51471280-51471299 None:intergenic 40.0%
! CCATATTTGCTACACACTTG+TGG - chr4_4:51471277-51471296 Msa0686860:five_prime_UTR 40.0%
!! TTGGAGCATATAGTGGGTGT+TGG + chr4_4:51468988-51469007 None:intergenic 45.0%
AAGCGGTTCTCTTCTCAGTA+AGG + chr4_4:51469064-51469083 None:intergenic 45.0%
TCTTGGACGCCATATTCAAG+CGG + chr4_4:51469081-51469100 None:intergenic 45.0%
GGCGTCCAAGAGAATAGTTA+AGG - chr4_4:51469090-51469109 Msa0686860:three_prime_UTR 45.0%
! TGATGAGTTTCAGGTCCAGT+CGG - chr4_4:51469223-51469242 Msa0686860:three_prime_UTR 45.0%
TCGGTGAGGACATGTTTCAT+TGG - chr4_4:51469242-51469261 Msa0686860:three_prime_UTR 45.0%
CCAGCATAGGGACTATCATT+TGG + chr4_4:51469286-51469305 None:intergenic 45.0%
CCAAATGATAGTCCCTATGC+TGG - chr4_4:51469283-51469302 Msa0686860:three_prime_UTR 45.0%
AATGATAGTCCCTATGCTGG+TGG - chr4_4:51469286-51469305 Msa0686860:three_prime_UTR 45.0%
AGGAAAACACCACCAGCATA+GGG + chr4_4:51469298-51469317 None:intergenic 45.0%
AAGGAAAACACCACCAGCAT+AGG + chr4_4:51469299-51469318 None:intergenic 45.0%
GGTTTGAAGGGATAATCAGG+AGG + chr4_4:51469337-51469356 None:intergenic 45.0%
GGAGGTTTGAAGGGATAATC+AGG + chr4_4:51469340-51469359 None:intergenic 45.0%
TTATCCCTTCAAACCTCCCA+AGG - chr4_4:51469342-51469361 Msa0686860:three_prime_UTR 45.0%
GTATACCTTGGGAGGTTTGA+AGG + chr4_4:51469350-51469369 None:intergenic 45.0%
! ACTTTGGTGTATACCTTGGG+AGG + chr4_4:51469358-51469377 None:intergenic 45.0%
! AGGGATTTGAGTTTGGACAC+GGG - chr4_4:51469542-51469561 Msa0686860:three_prime_UTR 45.0%
! GTCGTTAATTTGAGATCGGG+CGG + chr4_4:51469645-51469664 None:intergenic 45.0%
CTGTAACCGTAACTTGACAG+CGG - chr4_4:51469841-51469860 Msa0686860:three_prime_UTR 45.0%
CCTTGGATATTGTGAGAGCA+GGG + chr4_4:51470076-51470095 None:intergenic 45.0%
ACCTTGGATATTGTGAGAGC+AGG + chr4_4:51470077-51470096 None:intergenic 45.0%
CTAATGACGTCAACGAGGAA+TGG + chr4_4:51470114-51470133 None:intergenic 45.0%
ACAAGAGGGTCATCAGGATT+TGG + chr4_4:51470227-51470246 None:intergenic 45.0%
AAAGACGCGGCCTTGAAATT+TGG - chr4_4:51470383-51470402 Msa0686860:intron 45.0%
AAGACGCGGCCTTGAAATTT+GGG - chr4_4:51470384-51470403 Msa0686860:intron 45.0%
TCAGCACTGCCCAAATTTCA+AGG + chr4_4:51470396-51470415 None:intergenic 45.0%
GTTAGGGAGGAGTCAAGTTA+AGG + chr4_4:51470582-51470601 None:intergenic 45.0%
! ATGGTGGTGACAAAAGAAGG+TGG - chr4_4:51470695-51470714 Msa0686860:intron 45.0%
!! GATGGTGGTGATAATGGTGA+AGG + chr4_4:51470802-51470821 None:intergenic 45.0%
TGGTGGTCAGAATGATTGTG+TGG + chr4_4:51470878-51470897 None:intergenic 45.0%
TGAGTTAAGGAATGTGCTGG+TGG + chr4_4:51470895-51470914 None:intergenic 45.0%
CTGACGAAGGACTTGAGTTA+AGG + chr4_4:51470908-51470927 None:intergenic 45.0%
AATGTCCATCCTCTGCAAAC+CGG - chr4_4:51471014-51471033 Msa0686860:CDS 45.0%
!! ACACATGGGTTTGCTAGTTG+TGG - chr4_4:51471198-51471217 Msa0686860:CDS 45.0%
!!! TTTTTTTTTTTTTAAGAAAA+AGG - chr4_4:51469719-51469738 Msa0686860:three_prime_UTR 5.0%
TATGCTGTACCCACCTCCAT+TGG - chr4_4:51468912-51468931 Msa0686860:three_prime_UTR 50.0%
TTACCTGCACTGCATGAGGT+TGG + chr4_4:51469142-51469161 None:intergenic 50.0%
CTCTTTACCTGCACTGCATG+AGG + chr4_4:51469146-51469165 None:intergenic 50.0%
TGAAACATGTCCTCACCGAC+TGG + chr4_4:51469241-51469260 None:intergenic 50.0%
CCTCAACCGCTGTCAAGTTA+CGG + chr4_4:51469850-51469869 None:intergenic 50.0%
CCCTGCTCTCACAATATCCA+AGG - chr4_4:51470073-51470092 Msa0686860:CDS 50.0%
GAGCAACTTCAGGCACAAGA+GGG + chr4_4:51470241-51470260 None:intergenic 50.0%
TGAGCAACTTCAGGCACAAG+AGG + chr4_4:51470242-51470261 None:intergenic 50.0%
ATGAGTCCACTGCTAGAAGC+TGG - chr4_4:51470288-51470307 Msa0686860:CDS 50.0%
! AGTTTCAGGTCCAGTCGGTG+AGG - chr4_4:51469228-51469247 Msa0686860:three_prime_UTR 55.0%
CCGTAACTTGACAGCGGTTG+AGG - chr4_4:51469847-51469866 Msa0686860:three_prime_UTR 55.0%
GCAGGTTGCATTCAGGACCA+AGG - chr4_4:51469986-51470005 Msa0686860:three_prime_UTR 55.0%
TCAGGCACAAGAGGGTCATC+AGG + chr4_4:51470233-51470252 None:intergenic 55.0%
CTGAGTCCAGCTTCTAGCAG+TGG + chr4_4:51470297-51470316 None:intergenic 55.0%
!! AAGGACCGGTTTGCAGAGGA+TGG + chr4_4:51471022-51471041 None:intergenic 55.0%
!! CTAGAAGGACCGGTTTGCAG+AGG + chr4_4:51471026-51471045 None:intergenic 55.0%
CCTGCACTGCATGAGGTTGG+TGG + chr4_4:51469139-51469158 None:intergenic 60.0%
CCACCAACCTCATGCAGTGC+AGG - chr4_4:51469136-51469155 Msa0686860:three_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 51468799 51471543 51468799 ID=Msa0686860;Name=Msa0686860
chr4_4 mRNA 51468799 51471543 51468799 ID=Msa0686860-mRNA-1;Parent=Msa0686860;Name=Msa0686860-mRNA-1;_AED=0.07;_eAED=0.07;_QI=289|1|1|1|0.75|0.6|5|1131|148
chr4_4 exon 51468799 51469411 51468799 ID=Msa0686860-mRNA-1:exon:13289;Parent=Msa0686860-mRNA-1
chr4_4 exon 51469490 51470151 51469490 ID=Msa0686860-mRNA-1:exon:13288;Parent=Msa0686860-mRNA-1
chr4_4 exon 51470248 51470352 51470248 ID=Msa0686860-mRNA-1:exon:13287;Parent=Msa0686860-mRNA-1
chr4_4 exon 51470979 51471106 51470979 ID=Msa0686860-mRNA-1:exon:13286;Parent=Msa0686860-mRNA-1
chr4_4 exon 51471185 51471543 51471185 ID=Msa0686860-mRNA-1:exon:13285;Parent=Msa0686860-mRNA-1
chr4_4 five_prime_UTR 51471255 51471543 51471255 ID=Msa0686860-mRNA-1:five_prime_utr;Parent=Msa0686860-mRNA-1
chr4_4 CDS 51471185 51471254 51471185 ID=Msa0686860-mRNA-1:cds;Parent=Msa0686860-mRNA-1
chr4_4 CDS 51470979 51471106 51470979 ID=Msa0686860-mRNA-1:cds;Parent=Msa0686860-mRNA-1
chr4_4 CDS 51470248 51470352 51470248 ID=Msa0686860-mRNA-1:cds;Parent=Msa0686860-mRNA-1
chr4_4 CDS 51470008 51470151 51470008 ID=Msa0686860-mRNA-1:cds;Parent=Msa0686860-mRNA-1
chr4_4 three_prime_UTR 51469490 51470007 51469490 ID=Msa0686860-mRNA-1:three_prime_utr;Parent=Msa0686860-mRNA-1
chr4_4 three_prime_UTR 51468799 51469411 51468799 ID=Msa0686860-mRNA-1:three_prime_utr;Parent=Msa0686860-mRNA-1
Gene Sequence

>Msa0686860

ATGGCGTCCAAGAGAATAGTTAAGGAGCTTAAGGACTTGCAGAGAGATCCACCAACCTCATGCAGTGCAGGTCCAGTCGGTGAGGACATGTTTCATTGGCAAGCAACTATCATTGGTCCAAATGATAGTCCCTATGCTGGTGGTGTTTTCCTTGTCACCATTCATTTTCCTCCTGATTATCCCTTCAAACCTCCCAAGGTTGCATTCAGGACCAAGGTATTCCATCCCAACATTAACAGCAATGGCAATATTTGCCTAGACATACTCAAAGAACAATGGAGCCCTGCTCTCACAATATCCAAGGTACTGCTCTCCATATGTTCTCTGCTAACGGATCCAAATCCTGATGACCCTCTTGTGCCTGAAGTTGCTCATATGTGCAAGACAGACAGAGTCAAGTATGAGTCCACTGCTAGAAGCTGGACTCAGAAATTTGCTATGGGCTAA
Protein sequence

>Msa0686860

MASKRIVKELKDLQRDPPTSCSAGPVGEDMFHWQATIIGPNDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGNICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEVAHMCKTDRVKYESTARSWTQKFAMG*