AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0606640

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0606640 MtrunA17_Chr4g0037011 100.000 148 0 0 1 148 1 148 3.26e-110 309
Msa0606640 MtrunA17_Chr3g0108261 91.892 148 12 0 1 148 1 148 5.03e-103 291
Msa0606640 MtrunA17_Chr7g0275361 92.568 148 11 0 1 148 1 148 7.71e-103 290
Msa0606640 MtrunA17_Chr5g0432831 91.216 148 13 0 1 148 1 148 2.21e-102 289
Msa0606640 MtrunA17_Chr1g0154011 88.514 148 17 0 1 148 1 148 5.46e-100 283
Msa0606640 MtrunA17_Chr8g0369311 87.838 148 18 0 1 148 57 204 3.64e-98 281
Msa0606640 MtrunA17_Chr8g0378601 51.724 145 70 0 2 146 23 167 8.67e-55 169
Msa0606640 MtrunA17_Chr2g0316201 47.586 145 76 0 4 148 8 152 1.89e-46 148
Msa0606640 MtrunA17_Chr2g0316211 47.222 144 76 0 5 148 9 152 2.09e-45 145
Msa0606640 MtrunA17_Chr8g0362321 45.205 146 75 1 2 142 5 150 2.78e-44 142
Msa0606640 MtrunA17_Chr4g0060351 44.521 146 76 1 2 142 5 150 1.72e-43 140
Msa0606640 MtrunA17_Chr3g0131871 48.611 144 72 2 5 146 6 149 2.01e-40 134
Msa0606640 MtrunA17_Chr3g0139901 41.497 147 82 3 5 147 7 153 1.20e-34 118
Msa0606640 MtrunA17_Chr4g0038891 44.715 123 68 0 4 126 35 157 3.78e-34 117
Msa0606640 MtrunA17_Chr1g0199951 47.200 125 65 1 3 126 43 167 5.32e-34 117
Msa0606640 MtrunA17_Chr5g0396451 43.089 123 70 0 4 126 35 157 2.40e-32 113
Msa0606640 MtrunA17_Chr5g0403591 38.971 136 80 2 5 139 33 166 1.47e-28 103
Msa0606640 MtrunA17_Chr4g0053191 34.868 152 92 2 1 145 4 155 1.30e-27 100
Msa0606640 MtrunA17_Chr4g0031181 35.821 134 72 2 15 134 18 151 1.19e-26 98.2
Msa0606640 MtrunA17_Chr8g0348701 35.772 123 77 2 26 146 25 147 1.02e-25 96.3
Msa0606640 MtrunA17_Chr5g0401831 32.895 152 95 2 1 145 4 155 1.09e-25 95.5
Msa0606640 MtrunA17_Chr8g0387131 37.273 110 67 2 39 146 38 147 2.40e-24 92.8
Msa0606640 MtrunA17_Chr2g0328001 34.028 144 81 2 15 144 18 161 4.24e-24 91.7
Msa0606640 MtrunA17_Chr7g0254701 34.532 139 91 0 4 142 13 151 4.64e-24 94.0
Msa0606640 MtrunA17_Chr4g0059291 29.268 123 85 2 26 146 25 147 3.24e-21 84.7
Msa0606640 MtrunA17_Chr8g0372391 39.806 103 60 2 1 102 15 116 2.90e-19 79.0
Msa0606640 MtrunA17_Chr4g0068301 33.613 119 69 4 2 114 11 125 1.71e-17 77.0
Msa0606640 MtrunA17_Chr5g0404931 36.893 103 63 2 1 102 15 116 2.44e-17 73.9
Msa0606640 MtrunA17_Chr8g0376111 35.246 122 69 3 4 115 43 164 1.99e-16 74.3
Msa0606640 MtrunA17_Chr7g0233631 35.246 122 69 3 4 115 38 159 8.30e-16 72.8
Msa0606640 MtrunA17_Chr8g0345331 31.624 117 71 3 4 115 8 120 1.34e-15 71.2
Msa0606640 MtrunA17_Chr7g0233611 35.246 122 69 3 4 115 38 159 1.96e-15 71.6
Msa0606640 MtrunA17_Chr7g0222941 30.894 123 75 3 3 115 90 212 1.14e-14 68.6
Msa0606640 MtrunA17_Chr4g0047301 30.000 150 92 4 4 143 672 818 2.92e-14 68.9
Msa0606640 MtrunA17_Chr2g0282211 27.815 151 96 4 3 143 681 828 2.72e-13 66.2
Msa0606640 MtrunA17_Chr4g0045101 31.967 122 73 3 4 115 37 158 6.00e-13 64.7
Msa0606640 MtrunA17_Chr2g0317631 30.894 123 75 3 3 115 306 428 1.30e-12 63.9
Msa0606640 MtrunA17_Chr1g0186291 28.571 98 56 2 43 139 30 114 2.45e-12 60.5
Msa0606640 MtrunA17_Chr1g0198021 29.268 123 77 3 3 115 137 259 2.63e-12 63.2
Msa0606640 MtrunA17_Chr8g0376201 31.707 123 74 3 3 115 192 314 8.46e-12 61.6
Msa0606640 MtrunA17_Chr1g0211131 30.328 122 75 3 4 115 823 944 4.01e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0606640 AT1G64230.2 91.892 148 12 0 1 148 1 148 1.92e-103 292
Msa0606640 AT1G64230.1 91.892 148 12 0 1 148 1 148 1.92e-103 292
Msa0606640 AT5G53300.2 91.892 148 12 0 1 148 1 148 1.46e-102 290
Msa0606640 AT5G53300.4 91.892 148 12 0 1 148 1 148 1.46e-102 290
Msa0606640 AT5G53300.1 91.892 148 12 0 1 148 1 148 1.46e-102 290
Msa0606640 AT4G27960.1 91.216 148 13 0 1 148 1 148 3.48e-102 289
Msa0606640 AT5G41700.5 90.541 148 14 0 1 148 1 148 7.76e-102 288
Msa0606640 AT5G41700.2 90.541 148 14 0 1 148 1 148 7.76e-102 288
Msa0606640 AT5G41700.1 90.541 148 14 0 1 148 1 148 7.76e-102 288
Msa0606640 AT4G27960.2 91.216 148 13 0 1 148 31 178 2.30e-101 288
Msa0606640 AT3G08690.2 88.514 148 17 0 1 148 1 148 8.50e-100 283
Msa0606640 AT3G08690.1 88.514 148 17 0 1 148 1 148 8.50e-100 283
Msa0606640 AT5G56150.1 88.514 148 17 0 1 148 1 148 4.87e-99 281
Msa0606640 AT5G41700.4 89.262 149 15 1 1 148 1 149 6.21e-99 281
Msa0606640 AT1G64230.3 85.987 157 13 1 1 148 1 157 1.05e-98 281
Msa0606640 AT2G16740.1 85.714 147 21 0 1 147 1 147 3.36e-97 276
Msa0606640 AT1G64230.5 71.053 190 13 1 1 148 1 190 6.21e-94 270
Msa0606640 AT1G64230.4 71.053 190 13 1 1 148 1 190 6.21e-94 270
Msa0606640 AT3G08700.2 75.839 149 35 1 1 148 1 149 5.47e-84 243
Msa0606640 AT3G08700.1 75.839 149 35 1 1 148 1 149 5.47e-84 243
Msa0606640 AT5G41700.3 91.346 104 9 0 1 104 1 104 2.03e-69 206
Msa0606640 AT5G53300.3 89.908 109 9 1 1 107 1 109 2.19e-69 205
Msa0606640 AT5G56150.2 87.379 103 13 0 1 103 1 103 1.06e-65 196
Msa0606640 AT3G13550.1 48.966 145 74 0 2 146 37 181 1.51e-53 167
Msa0606640 AT1G16890.2 48.276 145 75 0 4 148 8 152 5.60e-47 149
Msa0606640 AT1G78870.2 47.586 145 76 0 4 148 8 152 3.70e-46 147
Msa0606640 AT5G62540.1 48.872 133 68 0 2 134 5 137 1.98e-44 143
Msa0606640 AT1G16890.3 46.104 154 74 1 4 148 8 161 2.98e-44 143
Msa0606640 AT2G02760.1 44.521 146 76 1 2 142 5 150 1.69e-43 140
Msa0606640 AT2G02760.2 44.521 146 76 1 2 142 5 150 1.69e-43 140
Msa0606640 AT2G02760.4 44.521 146 76 1 2 142 5 150 1.69e-43 140
Msa0606640 AT2G02760.3 44.521 146 76 1 2 142 5 150 1.69e-43 140
Msa0606640 AT1G14400.1 44.521 146 76 1 2 142 5 150 1.81e-43 140
Msa0606640 AT1G14400.2 44.521 146 76 1 2 142 5 150 1.81e-43 140
Msa0606640 AT3G13550.2 51.754 114 55 0 2 115 37 150 1.94e-43 141
Msa0606640 AT1G36340.2 46.897 145 76 1 2 146 9 152 4.65e-43 140
Msa0606640 AT1G36340.1 46.897 145 76 1 2 146 9 152 4.65e-43 140
Msa0606640 AT1G16890.1 50.420 119 59 0 30 148 1 119 5.99e-40 130
Msa0606640 AT1G78870.4 50.420 119 59 0 30 148 1 119 1.01e-39 130
Msa0606640 AT5G50870.1 47.917 144 73 2 5 146 6 149 2.10e-38 129
Msa0606640 AT1G78870.1 50.000 120 59 1 30 148 1 120 5.99e-38 125
Msa0606640 AT5G50870.2 44.516 155 73 3 5 146 6 160 1.87e-35 122
Msa0606640 AT3G24515.1 43.949 157 75 3 3 146 8 164 2.05e-33 121
Msa0606640 AT5G25760.1 40.136 147 84 3 5 147 7 153 4.42e-33 114
Msa0606640 AT5G25760.2 40.136 147 84 3 5 147 7 153 4.42e-33 114
Msa0606640 AT2G32790.1 51.587 126 57 2 25 148 54 177 9.54e-33 114
Msa0606640 AT1G78870.3 48.571 105 54 0 4 108 8 112 2.85e-32 111
Msa0606640 AT3G20060.1 43.089 123 70 0 4 126 39 161 7.90e-31 109
Msa0606640 AT3G24515.3 45.902 122 62 1 29 146 68 189 1.77e-30 114
Msa0606640 AT1G50490.1 43.902 123 69 0 4 126 38 160 2.34e-30 108
Msa0606640 AT3G20060.2 45.714 105 57 0 22 126 12 116 4.00e-29 103
Msa0606640 AT3G57870.1 35.526 152 91 3 1 145 5 156 1.90e-27 100
Msa0606640 AT4G36800.2 38.235 136 81 2 5 139 34 167 3.23e-27 100
Msa0606640 AT4G36800.1 38.235 136 81 2 5 139 34 167 3.23e-27 100
Msa0606640 AT2G46030.5 35.135 148 93 3 1 146 1 147 3.78e-27 100
Msa0606640 AT2G46030.1 35.135 148 93 3 1 146 1 147 3.78e-27 100
Msa0606640 AT5G05080.2 37.410 139 87 0 4 142 13 151 5.91e-27 101
Msa0606640 AT5G05080.3 37.410 139 87 0 4 142 13 151 5.91e-27 101
Msa0606640 AT5G05080.1 37.410 139 87 0 4 142 13 151 5.91e-27 101
Msa0606640 AT5G05080.4 37.410 139 87 0 4 142 13 151 5.91e-27 101
Msa0606640 AT2G46030.3 35.135 148 93 3 1 146 73 219 1.01e-26 100
Msa0606640 AT2G46030.4 38.211 123 74 2 26 146 28 150 1.03e-26 99.4
Msa0606640 AT2G46030.2 38.211 123 74 2 26 146 28 150 1.03e-26 99.4
Msa0606640 AT5G41340.1 36.585 123 76 2 26 146 25 147 7.01e-26 97.1
Msa0606640 AT1G63800.2 36.585 123 76 2 26 146 10 132 8.79e-26 96.3
Msa0606640 AT1G63800.1 36.585 123 76 2 26 146 25 147 9.53e-26 96.7
Msa0606640 AT3G55380.1 35.075 134 73 2 15 134 19 152 2.64e-25 95.1
Msa0606640 AT5G59300.1 36.296 135 72 2 15 135 18 152 1.09e-24 93.6
Msa0606640 AT3G46460.1 34.028 144 81 2 15 144 18 161 1.63e-24 93.2
Msa0606640 AT2G18600.1 36.496 137 82 4 5 139 35 168 3.13e-24 92.8
Msa0606640 AT2G18600.2 36.496 137 82 4 5 139 35 168 3.13e-24 92.8
Msa0606640 AT5G41340.2 38.462 104 62 2 45 146 9 112 1.02e-22 88.2
Msa0606640 AT3G17000.1 36.134 119 66 4 2 114 12 126 3.23e-19 82.0
Msa0606640 AT1G75440.1 38.835 103 61 2 1 102 15 116 6.27e-19 78.6
Msa0606640 AT3G55380.2 35.965 114 57 3 15 112 19 132 3.40e-18 77.4
Msa0606640 AT1G45050.1 37.864 103 62 2 1 102 15 116 4.93e-18 76.3
Msa0606640 AT5G42990.1 38.835 103 61 2 1 102 15 116 9.98e-18 75.5
Msa0606640 AT1G17280.9 33.333 117 69 3 4 115 8 120 1.03e-16 74.3
Msa0606640 AT1G17280.7 33.333 117 69 3 4 115 8 120 1.03e-16 74.3
Msa0606640 AT1G17280.6 33.333 117 69 3 4 115 8 120 1.03e-16 74.3
Msa0606640 AT1G17280.2 33.333 117 69 3 4 115 8 120 1.03e-16 74.3
Msa0606640 AT1G17280.5 33.333 117 69 3 4 115 8 120 1.03e-16 74.3
Msa0606640 AT1G17280.3 33.333 117 69 3 4 115 8 120 1.03e-16 74.3
Msa0606640 AT1G17280.1 33.333 117 69 3 4 115 8 120 1.03e-16 74.3
Msa0606640 AT1G17280.8 33.333 117 69 3 4 115 8 120 1.03e-16 74.3
Msa0606640 AT4G36410.1 35.185 108 64 3 1 107 15 117 1.34e-16 72.4
Msa0606640 AT5G50430.1 33.333 117 69 3 4 115 8 120 1.35e-16 74.3
Msa0606640 AT5G50430.2 33.333 117 69 3 4 115 8 120 1.35e-16 74.3
Msa0606640 AT3G46460.3 30.631 111 64 1 47 144 9 119 4.35e-16 70.1
Msa0606640 AT3G46460.2 30.631 111 64 1 47 144 9 119 4.35e-16 70.1
Msa0606640 AT1G17280.4 30.894 123 70 3 4 115 8 126 1.96e-15 70.9
Msa0606640 AT3G15355.1 29.139 151 94 4 3 143 334 481 7.24e-12 62.4
Msa0606640 AT1G53025.2 31.148 122 74 3 4 115 274 395 2.06e-11 60.8
Msa0606640 AT1G45050.2 34.831 89 56 2 1 88 15 102 2.09e-11 57.8
Msa0606640 AT1G53025.1 31.148 122 74 3 4 115 274 395 2.15e-11 60.8
Msa0606640 AT1G53023.1 31.148 122 74 3 4 115 60 181 9.35e-11 58.9

Find 115 sgRNAs with CRISPR-Local

Find 187 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTAATTATGAATCTAATT+TGG 0.125669 4_2:-53246130 Msa0606640:three_prime_UTR
AAAGACGCGGCCTTGAAATT+TGG 0.144554 4_2:-53247045 Msa0606640:three_prime_UTR
ATCTTGTGTTTCGTGCTTTA+TGG 0.171568 4_2:-53246358 Msa0606640:three_prime_UTR
GTTAGGGAGGAGTCAATTTA+AGG 0.226400 4_2:+53246849 None:intergenic
TGCACAAATTTATCCCTTCT+TGG 0.235806 4_2:+53246897 None:intergenic
GAATAAATTCTACTCTCTTT+TGG 0.250551 4_2:-53246953 Msa0606640:three_prime_UTR
CAGGTTGCAGGCATATTTAT+TGG 0.271991 4_2:+53248436 None:intergenic
ACTCTTGTTTAGTGTTTCAT+TGG 0.289354 4_2:-53246321 Msa0606640:three_prime_UTR
TAATGGTTGGATCGATTATA+TGG 0.292507 4_2:+53246525 None:intergenic
CCACAAGTGTGTAGCAAATA+TGG 0.302149 4_2:+53246175 None:intergenic
GTTAATTATGAATCTAATTT+GGG 0.305844 4_2:-53246129 Msa0606640:three_prime_UTR
AAGACGCGGCCTTGAAATTT+GGG 0.308012 4_2:-53247044 Msa0606640:three_prime_UTR
TGTGCAGAAAATAATAAATA+TGG 0.317449 4_2:+53246274 None:intergenic
AACATAACATTAGCAAAATA+TGG 0.320168 4_2:+53246090 None:intergenic
ATAGGAACATGTGGCCTAAT+TGG 0.333623 4_2:+53246590 None:intergenic
AGAAGAGAACCGCTTGAATA+TGG 0.338115 4_2:-53248371 Msa0606640:exon
CATTGGCAAGCAACCATCAT+TGG 0.350944 4_2:-53248179 Msa0606640:CDS
CATTTAGAAACCCTAAAATC+TGG 0.361988 4_2:-53246799 Msa0606640:three_prime_UTR
CACATAACATTTGCAAAATT+AGG 0.365332 4_2:+53246031 None:intergenic
GAGAATAGTTAAGGAGCTTA+AGG 0.366328 4_2:-53248341 Msa0606640:CDS
GGAGTTAGTCTTACAGTTGA+TGG 0.368318 4_2:+53246611 None:intergenic
AGATAAGATTACAAGAATAA+AGG 0.373866 4_2:+53248571 None:intergenic
GGCCTAAAAGTAGCTGTTAA+TGG 0.376008 4_2:+53246508 None:intergenic
CTGTAAGACTAACTCCAATT+AGG 0.395549 4_2:-53246604 Msa0606640:three_prime_UTR
GGAGGTTTGAAGGGATAATC+AGG 0.402929 4_2:+53248101 None:intergenic
ATAAATTTGTGCACTTCCTC+AGG 0.402934 4_2:-53246887 Msa0606640:three_prime_UTR
ACCTTGGATATTGTGAGAGC+AGG 0.413179 4_2:+53247354 None:intergenic
ACAAGAGGGTCATCAGGATT+TGG 0.424690 4_2:+53247204 None:intergenic
ATGAAGAATGAAGAACTAGA+AGG 0.425461 4_2:+53246421 None:intergenic
ACAGTTGATGGTGGTGATAA+TGG 0.425707 4_2:+53246623 None:intergenic
ATCCGTTAGCAGAGAACATA+TGG 0.433316 4_2:+53247227 None:intergenic
CCAGCATAGGGACTATCATT+TGG 0.439200 4_2:+53248155 None:intergenic
AAGCGGTTCTCTTCTCAGTA+AGG 0.442442 4_2:+53248379 None:intergenic
CAAGAAATAAAACAGGTTGC+AGG 0.442953 4_2:+53248424 None:intergenic
TCAGCACTGCCCAAATTTCA+AGG 0.443228 4_2:+53247035 None:intergenic
ATTCTGATTGCCATTGACTT+TGG 0.445051 4_2:-53246766 Msa0606640:three_prime_UTR
AAGCTCCTTAACTATTCTCT+TGG 0.446675 4_2:+53248345 None:intergenic
GTATACCTTGGGAGGTTTGA+AGG 0.451790 4_2:+53248091 None:intergenic
GGCAAAAGATGAGCATATCT+TGG 0.460613 4_2:+53246484 None:intergenic
AGCATATATTCCTCAAGAAA+AGG 0.462112 4_2:+53248547 None:intergenic
ATATTTATTGGAGCATATAG+TGG 0.466226 4_2:+53248448 None:intergenic
TGACTTTGGTTAACAATGAA+TGG 0.466799 4_2:-53246752 Msa0606640:three_prime_UTR
ATTGTTCTTTGAGTATGTCT+AGG 0.473064 4_2:+53247382 None:intergenic
TTGGAGCATATAGTGGGTGT+TGG 0.475454 4_2:+53248455 None:intergenic
TCAGGCACAAGAGGGTCATC+AGG 0.475511 4_2:+53247198 None:intergenic
ACTATCATTTGGACCAATGA+TGG 0.481239 4_2:+53248166 None:intergenic
GTTCTCTTCTCAGTAAGGAT+TGG 0.482658 4_2:+53248384 None:intergenic
TTGCACATATGAGCAACTTC+AGG 0.491907 4_2:+53247180 None:intergenic
TCGGTGAGGACATGTTTCAT+TGG 0.492631 4_2:-53248196 Msa0606640:CDS
CTCCATATGTTCTCTGCTAA+CGG 0.494453 4_2:-53247229 Msa0606640:CDS
TTGCCATTGCTGTTAATGTT+GGG 0.496480 4_2:+53247411 None:intergenic
CCTGCACTGCATGAGGTTGG+TGG 0.496861 4_2:+53248304 None:intergenic
CCACCAACCTCATGCAGTGC+AGG 0.500851 4_2:-53248304 Msa0606640:intron
TCATTGTTAACCAAAGTCAA+TGG 0.501243 4_2:+53246756 None:intergenic
CTGGACTCAGAAATTTGCTA+TGG 0.506602 4_2:-53247121 Msa0606640:CDS
AATGTCCATCCTCTGCAAAC+CGG 0.506871 4_2:-53246445 Msa0606640:three_prime_UTR
TGAAACATGTCCTCACCGAC+TGG 0.508985 4_2:+53248200 None:intergenic
CAAACTACAAGAAATAAAAC+AGG 0.510875 4_2:+53248417 None:intergenic
AAAGTCAATGGCAATCAGAA+TGG 0.512767 4_2:+53246768 None:intergenic
AAGGAAAACACCGCCAGCAT+AGG 0.514098 4_2:+53248142 None:intergenic
ATTGCCATTGCTGTTAATGT+TGG 0.518283 4_2:+53247410 None:intergenic
ATGAGTCCACTGCTAGAAGC+TGG 0.523736 4_2:-53247140 Msa0606640:CDS
TTACCTGCACTGCATGAGGT+TGG 0.530132 4_2:+53248301 None:intergenic
GGCGTCCAAGAGAATAGTTA+AGG 0.531160 4_2:-53248350 Msa0606640:CDS
TAAAAGTAGCTGTTAATGGT+TGG 0.531639 4_2:+53246512 None:intergenic
AAGGGCCGGTTTGCAGAGGA+TGG 0.537361 4_2:+53246440 None:intergenic
TATACCTTGGGAGGTTTGAA+GGG 0.541616 4_2:+53248092 None:intergenic
GAATGAAGAACTAGAAGGGC+CGG 0.545140 4_2:+53246426 None:intergenic
TATTTATTGGAGCATATAGT+GGG 0.545894 4_2:+53248449 None:intergenic
AGGAAAACACCGCCAGCATA+GGG 0.547017 4_2:+53248143 None:intergenic
TATGCTGTACCCACCTCCAT+TGG 0.547984 4_2:-53248530 Msa0606640:five_prime_UTR
CTCTTTACCTGCACTGCATG+AGG 0.548184 4_2:+53248297 None:intergenic
TGGGATGGAATACCTTGGTC+CGG 0.549448 4_2:+53247430 None:intergenic
CCAAATGATAGTCCCTATGC+TGG 0.551124 4_2:-53248155 Msa0606640:CDS
CCATATTTGCTACACACTTG+TGG 0.553112 4_2:-53246175 Msa0606640:three_prime_UTR
AATTATATCTTAGTTGAAAG+CGG 0.554861 4_2:-53248622 Msa0606640:five_prime_UTR
ACACATGGGTTTGCTAGTTG+TGG 0.564717 4_2:-53246254 Msa0606640:three_prime_UTR
CACGAAACACAAGATATATG+TGG 0.565358 4_2:+53246366 None:intergenic
CCTTGGATATTGTGAGAGCA+GGG 0.575241 4_2:+53247355 None:intergenic
TGAAGAATGAAGAACTAGAA+GGG 0.580430 4_2:+53246422 None:intergenic
CTGAGTCCAGCTTCTAGCAG+TGG 0.582567 4_2:+53247134 None:intergenic
AAAAGATGAGCATATCTTGG+AGG 0.585127 4_2:+53246487 None:intergenic
CTACTCATCGAATTACAGAA+TGG 0.587340 4_2:-53246393 Msa0606640:three_prime_UTR
TACAATAGTAACTTAAGTCT+AGG 0.589560 4_2:+53246297 None:intergenic
ACTTTGGTGTATACCTTGGG+AGG 0.590340 4_2:+53248083 None:intergenic
TTATCCCTTCAAACCTCCCA+AGG 0.591046 4_2:-53248096 Msa0606640:intron
ATAATCAGGAGGAAAATGAA+TGG 0.593685 4_2:+53248115 None:intergenic
CATTGCTGTTAATGTTGGGA+TGG 0.595351 4_2:+53247415 None:intergenic
TGGACTCAGAAATTTGCTAT+GGG 0.595611 4_2:-53247120 Msa0606640:CDS
CCCTGCTCTCACAATATCCA+AGG 0.597960 4_2:-53247355 Msa0606640:intron
TGAGCAACTTCAGGCACAAG+AGG 0.601737 4_2:+53247189 None:intergenic
CATCCCAACATTAACAGCAA+TGG 0.604832 4_2:-53247414 Msa0606640:CDS
TGAATGGTGGTGACAAAAGA+AGG 0.606788 4_2:-53246736 Msa0606640:three_prime_UTR
GCTTCAAATTTGTTGACCTG+AGG 0.612617 4_2:+53246871 None:intergenic
CTAGAAGGGCCGGTTTGCAG+AGG 0.615837 4_2:+53246436 None:intergenic
GCAGGTTGCATTCCGGACCA+AGG 0.620692 4_2:-53247442 Msa0606640:intron
TAGACATACTCAAAGAACAA+TGG 0.637759 4_2:-53247380 Msa0606640:CDS
TCTTGGACGCCATATTCAAG+CGG 0.638332 4_2:+53248362 None:intergenic
TGATGAGTTTCAGGTCCAGT+CGG 0.644910 4_2:-53248215 Msa0606640:intron
AATGATAGTCCCTATGCTGG+CGG 0.653459 4_2:-53248152 Msa0606640:CDS
GAGCAACTTCAGGCACAAGA+GGG 0.655538 4_2:+53247190 None:intergenic
AAAGTATTGAAGGAGACAAG+AGG 0.657206 4_2:+53247083 None:intergenic
GAGGAAAATGAATGGTGACA+AGG 0.659276 4_2:+53248123 None:intergenic
GTTGGAGAAGAGACAATGAA+AGG 0.664356 4_2:+53248473 None:intergenic
AGTTTCAGGTCCAGTCGGTG+AGG 0.671300 4_2:-53248210 Msa0606640:intron
AATGTTGGGATGGAATACCT+TGG 0.674072 4_2:+53247425 None:intergenic
AAGCATAAAGAATAAGACCA+TGG 0.678522 4_2:-53245989 Msa0606640:three_prime_UTR
CTCATCGAATTACAGAATGG+AGG 0.679157 4_2:-53246390 Msa0606640:three_prime_UTR
GATGGTGGTGATAATGGTGA+AGG 0.680477 4_2:+53246629 None:intergenic
GTTAGTCTTACAGTTGATGG+TGG 0.681350 4_2:+53246614 None:intergenic
CTTTGGTTAACAATGAATGG+TGG 0.689816 4_2:-53246749 Msa0606640:three_prime_UTR
TAGTAGACTTAAATATACCA+TGG 0.691861 4_2:+53245972 None:intergenic
GGTTTGAAGGGATAATCAGG+AGG 0.711960 4_2:+53248104 None:intergenic
ATGGTGGTGACAAAAGAAGG+TGG 0.718551 4_2:-53246733 Msa0606640:three_prime_UTR
TGTTTGAATTGTAAAAGACG+CGG 0.719191 4_2:-53247058 Msa0606640:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAATAAACCTATTATA+TGG + chr4_2:53246915-53246934 None:intergenic 10.0%
!!! TATGATTTGTTATGTATAAT+AGG - chr4_2:53246658-53246677 Msa0606640:three_prime_UTR 15.0%
!!! ATGATTTGTTATGTATAATA+GGG - chr4_2:53246659-53246678 Msa0606640:three_prime_UTR 15.0%
!!! AATATCAAGATTTTAAATCT+TGG + chr4_2:53247077-53247096 None:intergenic 15.0%
!!! TTCAGCATTTTTATATAAAA+CGG + chr4_2:53247290-53247309 None:intergenic 15.0%
!! TGTTAATTATGAATCTAATT+TGG - chr4_2:53248444-53248463 Msa0606640:five_prime_UTR 15.0%
!! GTTAATTATGAATCTAATTT+GGG - chr4_2:53248445-53248464 Msa0606640:five_prime_UTR 15.0%
!! AATTATATCTTAGTTGAAAG+CGG - chr4_2:53245952-53245971 Msa0606640:three_prime_UTR 20.0%
!! AGATAAGATTACAAGAATAA+AGG + chr4_2:53246006-53246025 None:intergenic 20.0%
!!! ATTTGTTGTTTTTTTACCAA+TGG + chr4_2:53246063-53246082 None:intergenic 20.0%
!! ATTGGTAAAAAAACAACAAA+TGG - chr4_2:53246062-53246081 Msa0606640:three_prime_UTR 20.0%
!! AAGACAAACATATCTATTTA+AGG + chr4_2:53246623-53246642 None:intergenic 20.0%
!!! AGGTTTTTTTTGTAAGAAAA+AGG - chr4_2:53246865-53246884 Msa0606640:three_prime_UTR 20.0%
!!! AGTATAAAAAATGATTTTGC+AGG - chr4_2:53247114-53247133 Msa0606640:exon 20.0%
!!! TTCAAACAAAAAAGTATTGA+AGG + chr4_2:53247504-53247523 None:intergenic 20.0%
!! TATTAAAAATGTACCAAGAA+GGG - chr4_2:53247664-53247683 Msa0606640:intron 20.0%
!! TGTGCAGAAAATAATAAATA+TGG + chr4_2:53248303-53248322 None:intergenic 20.0%
!! AACATAACATTAGCAAAATA+TGG + chr4_2:53248487-53248506 None:intergenic 20.0%
! GGCAAAAATACAAAGTAATA+TGG - chr4_2:53245973-53245992 Msa0606640:three_prime_UTR 25.0%
! TATTTATTGGAGCATATAGT+GGG + chr4_2:53246128-53246147 None:intergenic 25.0%
! ATATTTATTGGAGCATATAG+TGG + chr4_2:53246129-53246148 None:intergenic 25.0%
! CAAACTACAAGAAATAAAAC+AGG + chr4_2:53246160-53246179 None:intergenic 25.0%
! CTGGAATAAATAAAGAAAAC+AGG + chr4_2:53246537-53246556 None:intergenic 25.0%
! ACTATCTAAACTCACAATAA+CGG - chr4_2:53246713-53246732 Msa0606640:three_prime_UTR 25.0%
! AATAAACCTATTATATGGAC+TGG + chr4_2:53246910-53246929 None:intergenic 25.0%
! AAAAAGATCTGTTGTGATTT+AGG - chr4_2:53246938-53246957 Msa0606640:three_prime_UTR 25.0%
! GTTAAAAATCATGATGATAC+AGG - chr4_2:53247315-53247334 Msa0606640:intron 25.0%
!! GAATAAATTCTACTCTCTTT+TGG - chr4_2:53247621-53247640 Msa0606640:intron 25.0%
!! CTTCTTGGTACATTTTTAAT+AGG + chr4_2:53247665-53247684 None:intergenic 25.0%
! CTATTAAAAATGTACCAAGA+AGG - chr4_2:53247663-53247682 Msa0606640:intron 25.0%
! AGCACTAAAAACATGTATTT+GGG + chr4_2:53247903-53247922 None:intergenic 25.0%
! TAGCACTAAAAACATGTATT+TGG + chr4_2:53247904-53247923 None:intergenic 25.0%
! TACAATAGTAACTTAAGTCT+AGG + chr4_2:53248280-53248299 None:intergenic 25.0%
!! TTTATTATTTTCTGCACACA+TGG - chr4_2:53248305-53248324 Msa0606640:CDS 25.0%
!! TTATTATTTTCTGCACACAT+GGG - chr4_2:53248306-53248325 Msa0606640:CDS 25.0%
!! TGTTTATTGTTGTGTTTTGT+TGG - chr4_2:53248356-53248375 Msa0606640:exon 25.0%
! CACATAACATTTGCAAAATT+AGG + chr4_2:53248546-53248565 None:intergenic 25.0%
! TAGTAGACTTAAATATACCA+TGG + chr4_2:53248605-53248624 None:intergenic 25.0%
! ATCTTATCTTCCTTTTCTTG+AGG - chr4_2:53246017-53246036 Msa0606640:three_prime_UTR 30.0%
AGCATATATTCCTCAAGAAA+AGG + chr4_2:53246030-53246049 None:intergenic 30.0%
!!! TGTTGTTTTTTTACCAATGG+AGG + chr4_2:53246060-53246079 None:intergenic 30.0%
GTTTGAATGAAACTTTGAAC+TGG - chr4_2:53246299-53246318 Msa0606640:three_prime_UTR 30.0%
!! TGTTTGTTATGATGAGTTTC+AGG - chr4_2:53246350-53246369 Msa0606640:three_prime_UTR 30.0%
ATAATCAGGAGGAAAATGAA+TGG + chr4_2:53246462-53246481 None:intergenic 30.0%
!!! ACAGTGTTTTAAAAGCACTT+TGG + chr4_2:53246510-53246529 None:intergenic 30.0%
GTTAATAAGTGACTCTAGAT+AGG - chr4_2:53246566-53246585 Msa0606640:intron 30.0%
! GTATAATAGGGATTTGAGTT+TGG - chr4_2:53246671-53246690 Msa0606640:three_prime_UTR 30.0%
GTTATTGTGAGTTTAGATAG+TGG + chr4_2:53246714-53246733 None:intergenic 30.0%
AGTTACGGTTACAAATACAT+CGG + chr4_2:53246981-53247000 None:intergenic 30.0%
TGCAACTATCTGTAATATCA+AGG - chr4_2:53247036-53247055 Msa0606640:three_prime_UTR 30.0%
ATTGTTCTTTGAGTATGTCT+AGG + chr4_2:53247195-53247214 None:intergenic 30.0%
TAGACATACTCAAAGAACAA+TGG - chr4_2:53247194-53247213 Msa0606640:CDS 30.0%
TGTTTGAATTGTAAAAGACG+CGG - chr4_2:53247516-53247535 Msa0606640:intron 30.0%
! TGTGTCTTTTGCTTTAGTTA+GGG + chr4_2:53247744-53247763 None:intergenic 30.0%
! TTGTGTCTTTTGCTTTAGTT+AGG + chr4_2:53247745-53247764 None:intergenic 30.0%
CATTTAGAAACCCTAAAATC+TGG - chr4_2:53247775-53247794 Msa0606640:intron 30.0%
TCATTGTTAACCAAAGTCAA+TGG + chr4_2:53247821-53247840 None:intergenic 30.0%
TGACTTTGGTTAACAATGAA+TGG - chr4_2:53247822-53247841 Msa0606640:intron 30.0%
ACTAAAAACATGTATTTGGG+AGG + chr4_2:53247900-53247919 None:intergenic 30.0%
TAATGGTTGGATCGATTATA+TGG + chr4_2:53248052-53248071 None:intergenic 30.0%
TAAAAGTAGCTGTTAATGGT+TGG + chr4_2:53248065-53248084 None:intergenic 30.0%
!!! AACCATTAACAGCTACTTTT+AGG - chr4_2:53248064-53248083 Msa0606640:intron 30.0%
! ATCTTTTGCCTCAAAAAAGA+AGG - chr4_2:53248103-53248122 Msa0606640:CDS 30.0%
!!! ACATTTCTCCTTCTTTTTTG+AGG + chr4_2:53248114-53248133 None:intergenic 30.0%
TGAAGAATGAAGAACTAGAA+GGG + chr4_2:53248155-53248174 None:intergenic 30.0%
ATGAAGAATGAAGAACTAGA+AGG + chr4_2:53248156-53248175 None:intergenic 30.0%
! ACTCTTGTTTAGTGTTTCAT+TGG - chr4_2:53248253-53248272 Msa0606640:intron 30.0%
AAGCATAAAGAATAAGACCA+TGG - chr4_2:53248585-53248604 Msa0606640:five_prime_UTR 30.0%
!!! GTTTTTTTACCAATGGAGGT+GGG + chr4_2:53246056-53246075 None:intergenic 35.0%
!!! TGTTTTTTTACCAATGGAGG+TGG + chr4_2:53246057-53246076 None:intergenic 35.0%
CAAGAAATAAAACAGGTTGC+AGG + chr4_2:53246153-53246172 None:intergenic 35.0%
AAGCTCCTTAACTATTCTCT+TGG + chr4_2:53246232-53246251 None:intergenic 35.0%
GAGAATAGTTAAGGAGCTTA+AGG - chr4_2:53246233-53246252 Msa0606640:three_prime_UTR 35.0%
ACTATCATTTGGACCAATGA+TGG + chr4_2:53246411-53246430 None:intergenic 35.0%
ACTTATTAACAAGATGCAGC+TGG + chr4_2:53246556-53246575 None:intergenic 35.0%
TAAGGCTATCACACACAAAA+AGG + chr4_2:53246605-53246624 None:intergenic 35.0%
! TCAGTCGTTAATTTGAGATC+GGG + chr4_2:53246784-53246803 None:intergenic 35.0%
! CTCAGTCGTTAATTTGAGAT+CGG + chr4_2:53246785-53246804 None:intergenic 35.0%
!!! TTTTTTACTGCAGTAGATCC+TGG - chr4_2:53246832-53246851 Msa0606640:three_prime_UTR 35.0%
!! AAGGTTTGCTGTTGCAATTT+AGG - chr4_2:53246884-53246903 Msa0606640:three_prime_UTR 35.0%
TTTAGGCCAGTCCATATAAT+AGG - chr4_2:53246901-53246920 Msa0606640:three_prime_UTR 35.0%
! TTGCCATTGCTGTTAATGTT+GGG + chr4_2:53247166-53247185 None:intergenic 35.0%
! ATTGCCATTGCTGTTAATGT+TGG + chr4_2:53247167-53247186 None:intergenic 35.0%
!! TGGACTCAGAAATTTGCTAT+GGG - chr4_2:53247454-53247473 Msa0606640:intron 35.0%
!! AAAGTATTGAAGGAGACAAG+AGG + chr4_2:53247494-53247513 None:intergenic 35.0%
TGCACAAATTTATCCCTTCT+TGG + chr4_2:53247680-53247699 None:intergenic 35.0%
ATAAATTTGTGCACTTCCTC+AGG - chr4_2:53247687-53247706 Msa0606640:intron 35.0%
! GGAAATGAAGCCAGATTTTA+GGG + chr4_2:53247788-53247807 None:intergenic 35.0%
! TGGAAATGAAGCCAGATTTT+AGG + chr4_2:53247789-53247808 None:intergenic 35.0%
AAAGTCAATGGCAATCAGAA+TGG + chr4_2:53247809-53247828 None:intergenic 35.0%
!! ATTCTGATTGCCATTGACTT+TGG - chr4_2:53247808-53247827 Msa0606640:intron 35.0%
CTTTGGTTAACAATGAATGG+TGG - chr4_2:53247825-53247844 Msa0606640:intron 35.0%
CTGTAAGACTAACTCCAATT+AGG - chr4_2:53247970-53247989 Msa0606640:intron 35.0%
AATGATTGTGTGGAAGTTTG+TGG + chr4_2:53248014-53248033 None:intergenic 35.0%
AAAAGATGAGCATATCTTGG+AGG + chr4_2:53248090-53248109 None:intergenic 35.0%
CTACTCATCGAATTACAGAA+TGG - chr4_2:53248181-53248200 Msa0606640:CDS 35.0%
CACGAAACACAAGATATATG+TGG + chr4_2:53248211-53248230 None:intergenic 35.0%
ATCTTGTGTTTCGTGCTTTA+TGG - chr4_2:53248216-53248235 Msa0606640:intron 35.0%
GTTGGAGAAGAGACAATGAA+AGG + chr4_2:53246104-53246123 None:intergenic 40.0%
CAGGTTGCAGGCATATTTAT+TGG + chr4_2:53246141-53246160 None:intergenic 40.0%
GTTCTCTTCTCAGTAAGGAT+TGG + chr4_2:53246193-53246212 None:intergenic 40.0%
AGAAGAGAACCGCTTGAATA+TGG - chr4_2:53246203-53246222 Msa0606640:three_prime_UTR 40.0%
GAGGAAAATGAATGGTGACA+AGG + chr4_2:53246454-53246473 None:intergenic 40.0%
TATACCTTGGGAGGTTTGAA+GGG + chr4_2:53246485-53246504 None:intergenic 40.0%
! AGCACTTTGGTGTATACCTT+GGG + chr4_2:53246497-53246516 None:intergenic 40.0%
! AAGCACTTTGGTGTATACCT+TGG + chr4_2:53246498-53246517 None:intergenic 40.0%
! TAGGGATTTGAGTTTGGACA+CGG - chr4_2:53246677-53246696 Msa0606640:three_prime_UTR 40.0%
AAAAACCTGTTGTACAGACC+AGG + chr4_2:53246853-53246872 None:intergenic 40.0%
CATCGGAAATCTTGATGTCA+AGG + chr4_2:53246964-53246983 None:intergenic 40.0%
TTGTAACCGTAACTTGACAG+CGG - chr4_2:53246987-53247006 Msa0606640:three_prime_UTR 40.0%
! TGATTTTGCAGGTTGCATTC+CGG - chr4_2:53247125-53247144 Msa0606640:CDS 40.0%
AATGTTGGGATGGAATACCT+TGG + chr4_2:53247152-53247171 None:intergenic 40.0%
! CATTGCTGTTAATGTTGGGA+TGG + chr4_2:53247162-53247181 None:intergenic 40.0%
CATCCCAACATTAACAGCAA+TGG - chr4_2:53247160-53247179 Msa0606640:CDS 40.0%
GGTTCACAATAACATGACCT+TGG + chr4_2:53247239-53247258 None:intergenic 40.0%
AGAAACTAATGACGTCAACG+AGG + chr4_2:53247265-53247284 None:intergenic 40.0%
CTCCATATGTTCTCTGCTAA+CGG - chr4_2:53247345-53247364 Msa0606640:intron 40.0%
ATCCGTTAGCAGAGAACATA+TGG + chr4_2:53247350-53247369 None:intergenic 40.0%
TTGCACATATGAGCAACTTC+AGG + chr4_2:53247397-53247416 None:intergenic 40.0%
!! CTGGACTCAGAAATTTGCTA+TGG - chr4_2:53247453-53247472 Msa0606640:intron 40.0%
!! GCTTCAAATTTGTTGACCTG+AGG + chr4_2:53247706-53247725 None:intergenic 40.0%
GTTAGGGAGGAGTCAATTTA+AGG + chr4_2:53247728-53247747 None:intergenic 40.0%
! GTCTTTTGCTTTAGTTAGGG+AGG + chr4_2:53247741-53247760 None:intergenic 40.0%
! TGAATGGTGGTGACAAAAGA+AGG - chr4_2:53247838-53247857 Msa0606640:intron 40.0%
!! ACAGTTGATGGTGGTGATAA+TGG + chr4_2:53247954-53247973 None:intergenic 40.0%
!! GTTAGTCTTACAGTTGATGG+TGG + chr4_2:53247963-53247982 None:intergenic 40.0%
!! GGAGTTAGTCTTACAGTTGA+TGG + chr4_2:53247966-53247985 None:intergenic 40.0%
ATAGGAACATGTGGCCTAAT+TGG + chr4_2:53247987-53248006 None:intergenic 40.0%
!! TGTGGTGTTATAGGAACATG+TGG + chr4_2:53247996-53248015 None:intergenic 40.0%
!! GTGGAAGTTTGTGGTGTTAT+AGG + chr4_2:53248005-53248024 None:intergenic 40.0%
GGCCTAAAAGTAGCTGTTAA+TGG + chr4_2:53248069-53248088 None:intergenic 40.0%
GGCAAAAGATGAGCATATCT+TGG + chr4_2:53248093-53248112 None:intergenic 40.0%
CTCATCGAATTACAGAATGG+AGG - chr4_2:53248184-53248203 Msa0606640:CDS 40.0%
CCACAAGTGTGTAGCAAATA+TGG + chr4_2:53248402-53248421 None:intergenic 40.0%
! CCATATTTGCTACACACTTG+TGG - chr4_2:53248399-53248418 Msa0606640:five_prime_UTR 40.0%
!! TTGGAGCATATAGTGGGTGT+TGG + chr4_2:53246122-53246141 None:intergenic 45.0%
AAGCGGTTCTCTTCTCAGTA+AGG + chr4_2:53246198-53246217 None:intergenic 45.0%
TCTTGGACGCCATATTCAAG+CGG + chr4_2:53246215-53246234 None:intergenic 45.0%
GGCGTCCAAGAGAATAGTTA+AGG - chr4_2:53246224-53246243 Msa0606640:three_prime_UTR 45.0%
! TGATGAGTTTCAGGTCCAGT+CGG - chr4_2:53246359-53246378 Msa0606640:three_prime_UTR 45.0%
TCGGTGAGGACATGTTTCAT+TGG - chr4_2:53246378-53246397 Msa0606640:three_prime_UTR 45.0%
CATTGGCAAGCAACCATCAT+TGG - chr4_2:53246395-53246414 Msa0606640:three_prime_UTR 45.0%
CCAGCATAGGGACTATCATT+TGG + chr4_2:53246422-53246441 None:intergenic 45.0%
CCAAATGATAGTCCCTATGC+TGG - chr4_2:53246419-53246438 Msa0606640:three_prime_UTR 45.0%
AATGATAGTCCCTATGCTGG+CGG - chr4_2:53246422-53246441 Msa0606640:three_prime_UTR 45.0%
GGTTTGAAGGGATAATCAGG+AGG + chr4_2:53246473-53246492 None:intergenic 45.0%
GGAGGTTTGAAGGGATAATC+AGG + chr4_2:53246476-53246495 None:intergenic 45.0%
TTATCCCTTCAAACCTCCCA+AGG - chr4_2:53246478-53246497 Msa0606640:three_prime_UTR 45.0%
GTATACCTTGGGAGGTTTGA+AGG + chr4_2:53246486-53246505 None:intergenic 45.0%
! ACTTTGGTGTATACCTTGGG+AGG + chr4_2:53246494-53246513 None:intergenic 45.0%
! AGGGATTTGAGTTTGGACAC+GGG - chr4_2:53246678-53246697 Msa0606640:three_prime_UTR 45.0%
! GTCGTTAATTTGAGATCGGG+CGG + chr4_2:53246781-53246800 None:intergenic 45.0%
TAGATCCTGGTCTGTACAAC+AGG - chr4_2:53246845-53246864 Msa0606640:three_prime_UTR 45.0%
CCTTGGATATTGTGAGAGCA+GGG + chr4_2:53247222-53247241 None:intergenic 45.0%
ACCTTGGATATTGTGAGAGC+AGG + chr4_2:53247223-53247242 None:intergenic 45.0%
CTAATGACGTCAACGAGGAA+TGG + chr4_2:53247260-53247279 None:intergenic 45.0%
ACAAGAGGGTCATCAGGATT+TGG + chr4_2:53247373-53247392 None:intergenic 45.0%
AAAGACGCGGCCTTGAAATT+TGG - chr4_2:53247529-53247548 Msa0606640:intron 45.0%
AAGACGCGGCCTTGAAATTT+GGG - chr4_2:53247530-53247549 Msa0606640:intron 45.0%
TCAGCACTGCCCAAATTTCA+AGG + chr4_2:53247542-53247561 None:intergenic 45.0%
! ATGGTGGTGACAAAAGAAGG+TGG - chr4_2:53247841-53247860 Msa0606640:intron 45.0%
!! GATGGTGGTGATAATGGTGA+AGG + chr4_2:53247948-53247967 None:intergenic 45.0%
GCTGACGAAGAATGATTGTG+TGG + chr4_2:53248024-53248043 None:intergenic 45.0%
AATGTCCATCCTCTGCAAAC+CGG - chr4_2:53248129-53248148 Msa0606640:CDS 45.0%
GAATGAAGAACTAGAAGGGC+CGG + chr4_2:53248151-53248170 None:intergenic 45.0%
!! ACACATGGGTTTGCTAGTTG+TGG - chr4_2:53248320-53248339 Msa0606640:CDS 45.0%
TATGCTGTACCCACCTCCAT+TGG - chr4_2:53246044-53246063 Msa0606640:three_prime_UTR 50.0%
TTACCTGCACTGCATGAGGT+TGG + chr4_2:53246276-53246295 None:intergenic 50.0%
CTCTTTACCTGCACTGCATG+AGG + chr4_2:53246280-53246299 None:intergenic 50.0%
TGAAACATGTCCTCACCGAC+TGG + chr4_2:53246377-53246396 None:intergenic 50.0%
AGGAAAACACCGCCAGCATA+GGG + chr4_2:53246434-53246453 None:intergenic 50.0%
AAGGAAAACACCGCCAGCAT+AGG + chr4_2:53246435-53246454 None:intergenic 50.0%
CCTCAACCGCTGTCAAGTTA+CGG + chr4_2:53246996-53247015 None:intergenic 50.0%
TGGGATGGAATACCTTGGTC+CGG + chr4_2:53247147-53247166 None:intergenic 50.0%
CCCTGCTCTCACAATATCCA+AGG - chr4_2:53247219-53247238 Msa0606640:CDS 50.0%
GAGCAACTTCAGGCACAAGA+GGG + chr4_2:53247387-53247406 None:intergenic 50.0%
TGAGCAACTTCAGGCACAAG+AGG + chr4_2:53247388-53247407 None:intergenic 50.0%
ATGAGTCCACTGCTAGAAGC+TGG - chr4_2:53247434-53247453 Msa0606640:CDS 50.0%
! AGTTTCAGGTCCAGTCGGTG+AGG - chr4_2:53246364-53246383 Msa0606640:three_prime_UTR 55.0%
CCGTAACTTGACAGCGGTTG+AGG - chr4_2:53246993-53247012 Msa0606640:three_prime_UTR 55.0%
TCAGGCACAAGAGGGTCATC+AGG + chr4_2:53247379-53247398 None:intergenic 55.0%
CTGAGTCCAGCTTCTAGCAG+TGG + chr4_2:53247443-53247462 None:intergenic 55.0%
CCTGCACTGCATGAGGTTGG+TGG + chr4_2:53246273-53246292 None:intergenic 60.0%
CCACCAACCTCATGCAGTGC+AGG - chr4_2:53246270-53246289 Msa0606640:three_prime_UTR 60.0%
GCAGGTTGCATTCCGGACCA+AGG - chr4_2:53247132-53247151 Msa0606640:CDS 60.0%
!! AAGGGCCGGTTTGCAGAGGA+TGG + chr4_2:53248137-53248156 None:intergenic 60.0%
!! CTAGAAGGGCCGGTTTGCAG+AGG + chr4_2:53248141-53248160 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 53245931 53248665 53245931 ID=Msa0606640;Name=Msa0606640
chr4_2 mRNA 53245931 53248665 53245931 ID=Msa0606640-mRNA-1;Parent=Msa0606640;Name=Msa0606640-mRNA-1;_AED=0.29;_eAED=0.29;_QI=291|1|1|1|0.75|0.6|5|1138|148
chr4_2 exon 53245931 53246550 53245931 ID=Msa0606640-mRNA-1:exon:13093;Parent=Msa0606640-mRNA-1
chr4_2 exon 53246598 53247259 53246598 ID=Msa0606640-mRNA-1:exon:13092;Parent=Msa0606640-mRNA-1
chr4_2 exon 53247356 53247460 53247356 ID=Msa0606640-mRNA-1:exon:13091;Parent=Msa0606640-mRNA-1
chr4_2 exon 53248097 53248224 53248097 ID=Msa0606640-mRNA-1:exon:13090;Parent=Msa0606640-mRNA-1
chr4_2 exon 53248305 53248665 53248305 ID=Msa0606640-mRNA-1:exon:13089;Parent=Msa0606640-mRNA-1
chr4_2 five_prime_UTR 53248375 53248665 53248375 ID=Msa0606640-mRNA-1:five_prime_utr;Parent=Msa0606640-mRNA-1
chr4_2 CDS 53248305 53248374 53248305 ID=Msa0606640-mRNA-1:cds;Parent=Msa0606640-mRNA-1
chr4_2 CDS 53248097 53248224 53248097 ID=Msa0606640-mRNA-1:cds;Parent=Msa0606640-mRNA-1
chr4_2 CDS 53247356 53247460 53247356 ID=Msa0606640-mRNA-1:cds;Parent=Msa0606640-mRNA-1
chr4_2 CDS 53247116 53247259 53247116 ID=Msa0606640-mRNA-1:cds;Parent=Msa0606640-mRNA-1
chr4_2 three_prime_UTR 53246598 53247115 53246598 ID=Msa0606640-mRNA-1:three_prime_utr;Parent=Msa0606640-mRNA-1
chr4_2 three_prime_UTR 53245931 53246550 53245931 ID=Msa0606640-mRNA-1:three_prime_utr;Parent=Msa0606640-mRNA-1
Gene Sequence

>Msa0606640

ATGGCGTCCAAGAGAATAGTTAAGGAGCTTAAGGACTTGCAGAGAGATCCACCAACCTCATGCAGTGCAGGTCCAGTCGGTGAGGACATGTTTCATTGGCAAGCAACCATCATTGGTCCAAATGATAGTCCCTATGCTGGCGGTGTTTTCCTTGTCACCATTCATTTTCCTCCTGATTATCCCTTCAAACCTCCCAAGGTTGCATTCCGGACCAAGGTATTCCATCCCAACATTAACAGCAATGGCAATATTTGCCTAGACATACTCAAAGAACAATGGAGCCCTGCTCTCACAATATCCAAGGTACTGCTCTCCATATGTTCTCTGCTAACGGATCCAAATCCTGATGACCCTCTTGTGCCTGAAGTTGCTCATATGTGCAAGACAGACAGAGTCAAGTATGAGTCCACTGCTAGAAGCTGGACTCAGAAATTTGCTATGGGCTAA
Protein sequence

>Msa0606640

MASKRIVKELKDLQRDPPTSCSAGPVGEDMFHWQATIIGPNDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGNICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEVAHMCKTDRVKYESTARSWTQKFAMG*