AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0738720

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0738720 MtrunA17_Chr5g0438181 100.000 69 0 0 1 69 1 69 1.09e-44 139
Msa0738720 MtrunA17_Chr4g0071611 100.000 69 0 0 1 69 1 69 1.72e-44 139
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0738720 AT4G24920.1 92.647 68 5 0 1 68 1 68 4.96e-41 128
Msa0738720 AT5G50460.1 92.647 68 5 0 1 68 1 68 4.96e-41 128
Msa0738720 AT3G48570.1 86.957 69 9 0 1 69 1 69 2.17e-39 124

Find 38 sgRNAs with CRISPR-Local

Find 224 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTCTCAATTCACGCGAAAA+TGG 0.229980 5_1:-56578243 Msa0738720:exon
AAATATGAGAAATTGAAATT+AGG 0.251273 5_1:+56578269 None:intergenic
CACTGAATGTTCTATTGAAA+TGG 0.316272 5_1:+56576425 None:intergenic
AGATACTCTGGTGAGTAAAT+AGG 0.348236 5_1:-56576170 Msa0738720:three_prime_UTR
AACGAGCCTAACGCTGTCTT+TGG 0.352610 5_1:+56578181 None:intergenic
TTATCTATATTTCTTATCTT+CGG 0.410507 5_1:-56576371 Msa0738720:three_prime_UTR
TACCTTTGCGATCGGGTTTG+TGG 0.417825 5_1:+56578150 None:intergenic
CCAACGATGATGTTGTTGAT+TGG 0.436566 5_1:+56577957 None:intergenic
ACATGTTACATAAAACTGTA+AGG 0.442953 5_1:+56576341 None:intergenic
CATCATCGTTGGATCTGGTT+AGG 0.451612 5_1:-56577946 Msa0738720:intron
GGGGTTATACCTTTGCGATC+GGG 0.456406 5_1:+56578143 None:intergenic
AACAACATCATCGTTGGATC+TGG 0.457089 5_1:-56577951 Msa0738720:CDS
TGGATCGAAGACGGAATCAA+TGG 0.467824 5_1:+56578217 None:intergenic
AGGGGTTATACCTTTGCGAT+CGG 0.473227 5_1:+56578142 None:intergenic
GATTATTTCTGCAGGATGAC+GGG 0.476757 5_1:-56576498 Msa0738720:intron
TTTGTTGTGATGGGATTCGT+TGG 0.478676 5_1:-56578005 Msa0738720:CDS
GTTGCTGTTCGTACCGCGAT+TGG 0.481738 5_1:-56578029 Msa0738720:CDS
GGTGAAGGAAGCAGATGAAT+TGG 0.489333 5_1:+56578312 None:intergenic
CGCGGTACGAACAGCAACCT+TGG 0.492229 5_1:+56578034 None:intergenic
TTTCTAAAGATTAGATACTC+TGG 0.509291 5_1:-56576182 Msa0738720:three_prime_UTR
TGATTATTTCTGCAGGATGA+CGG 0.511253 5_1:-56576499 Msa0738720:intron
TTCTCTGAGTGGATCGAAGA+CGG 0.515282 5_1:+56578208 None:intergenic
ACTGAATGTTCTATTGAAAT+GGG 0.522320 5_1:+56576426 None:intergenic
CCTCAGGAAAGTTGTGAAGC+AGG 0.527193 5_1:-56576464 Msa0738720:three_prime_UTR
TTGTTGTGATGGGATTCGTT+GGG 0.527754 5_1:-56578004 Msa0738720:CDS
AAAGAGAAAACAAATTCGGA+TGG 0.529021 5_1:+56578356 None:intergenic
CCAATCAACAACATCATCGT+TGG 0.538152 5_1:-56577957 Msa0738720:CDS
GAAAGTTGTGAAGCAGGAGG+AGG 0.550624 5_1:-56576458 Msa0738720:three_prime_UTR
ACGGGAATTACAATCGCCTC+AGG 0.556837 5_1:-56576480 Msa0738720:three_prime_UTR
AATTCGCCAAAGACAGCGTT+AGG 0.569841 5_1:-56578187 Msa0738720:CDS
TTGAAATTAGGAGAATGAGA+AGG 0.584098 5_1:+56578281 None:intergenic
AAGAGAAAACAAATTCGGAT+GGG 0.611419 5_1:+56578357 None:intergenic
CCTGCTTCACAACTTTCCTG+AGG 0.626602 5_1:+56576464 None:intergenic
TGCCACAAACCCGATCGCAA+AGG 0.648616 5_1:-56578152 Msa0738720:intron
AGGTCAAAAGTCTCAACACA+TGG 0.662789 5_1:+56576304 None:intergenic
CAGGAAAGTTGTGAAGCAGG+AGG 0.667526 5_1:-56576461 Msa0738720:three_prime_UTR
CATCACAACAAAACCAATCG+CGG 0.679795 5_1:+56578016 None:intergenic
TCTTTGGCGAATTCTCTGAG+TGG 0.681670 5_1:+56578197 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTGAAAAGAAAAAAAAAAA+TGG - chr5_1:56576970-56576989 Msa0738720:intron 10.0%
!! TTTGAAAAGAAAAAAAAAAA+TGG - chr5_1:56576970-56576989 Msa0738720:intron 10.0%
!! AAATATGAGAAATTGAAATT+AGG + chr5_1:56576256-56576275 None:intergenic 15.0%
!!! TTGATTTGAATGAAATATTT+TGG - chr5_1:56576675-56576694 Msa0738720:intron 15.0%
!!! TTTTATAATGTTCATCTAAA+TGG - chr5_1:56577294-56577313 Msa0738720:intron 15.0%
!! ACAAAATAAACATTATATTG+AGG + chr5_1:56577838-56577857 None:intergenic 15.0%
!!! ATAATGTTTATTTTGTCTTT+TGG - chr5_1:56577842-56577861 Msa0738720:intron 15.0%
!! TTATCTATATTTCTTATCTT+CGG - chr5_1:56578151-56578170 Msa0738720:intron 15.0%
!! AAATATGAGAAATTGAAATT+AGG + chr5_1:56576256-56576275 None:intergenic 15.0%
!!! TTGATTTGAATGAAATATTT+TGG - chr5_1:56576675-56576694 Msa0738720:intron 15.0%
!!! TTTTATAATGTTCATCTAAA+TGG - chr5_1:56577294-56577313 Msa0738720:intron 15.0%
!! ACAAAATAAACATTATATTG+AGG + chr5_1:56577838-56577857 None:intergenic 15.0%
!!! ATAATGTTTATTTTGTCTTT+TGG - chr5_1:56577842-56577861 Msa0738720:intron 15.0%
!! TTATCTATATTTCTTATCTT+CGG - chr5_1:56578151-56578170 Msa0738720:intron 15.0%
!! TAAATCGAATAGAGAATTTA+GGG + chr5_1:56576402-56576421 None:intergenic 20.0%
!! TGATTTAAATCTCTATATTG+TGG - chr5_1:56576424-56576443 Msa0738720:three_prime_UTR 20.0%
!!! ATTTGAATGAAATATTTTGG+TGG - chr5_1:56576678-56576697 Msa0738720:intron 20.0%
!!! ATTTTTTGTATGATTTGCAA+TGG - chr5_1:56577177-56577196 Msa0738720:intron 20.0%
!! AAACATATTTGTGACAATAA+CGG - chr5_1:56577430-56577449 Msa0738720:intron 20.0%
!! TAAATCGAATAGAGAATTTA+GGG + chr5_1:56576402-56576421 None:intergenic 20.0%
!! TGATTTAAATCTCTATATTG+TGG - chr5_1:56576424-56576443 Msa0738720:three_prime_UTR 20.0%
!!! ATTTGAATGAAATATTTTGG+TGG - chr5_1:56576678-56576697 Msa0738720:intron 20.0%
!!! ATTTTTTGTATGATTTGCAA+TGG - chr5_1:56577177-56577196 Msa0738720:intron 20.0%
!! AAACATATTTGTGACAATAA+CGG - chr5_1:56577430-56577449 Msa0738720:intron 20.0%
! CGAAAAAGAGAAAACAAATT+CGG + chr5_1:56576173-56576192 None:intergenic 25.0%
! AAATCGAATAGAGAATTTAG+GGG + chr5_1:56576401-56576420 None:intergenic 25.0%
! GTAAATCGAATAGAGAATTT+AGG + chr5_1:56576403-56576422 None:intergenic 25.0%
!!! AATGAAATATTTTGGTGGAA+TGG - chr5_1:56576683-56576702 Msa0738720:intron 25.0%
! TGAATTTCTGATGTAAATGT+TGG - chr5_1:56576712-56576731 Msa0738720:intron 25.0%
! GAAACAATTTGATTGAATCA+AGG - chr5_1:56576741-56576760 Msa0738720:intron 25.0%
!!! AATCAAGGATATTTTGAAGT+GGG - chr5_1:56576756-56576775 Msa0738720:intron 25.0%
!! GGTTTTTGTAAGATTTACAA+TGG - chr5_1:56576825-56576844 Msa0738720:intron 25.0%
! TTGAGATCTTAATATGAATC+AGG - chr5_1:56577079-56577098 Msa0738720:intron 25.0%
! TGAGATCTTAATATGAATCA+GGG - chr5_1:56577080-56577099 Msa0738720:intron 25.0%
! ACAAGTAAATCCATTATGTT+AGG + chr5_1:56577153-56577172 None:intergenic 25.0%
! TGTTCTTAGTTATGATTGAT+TGG - chr5_1:56577247-56577266 Msa0738720:intron 25.0%
! CAAATTAGAAACTTCGAAAT+AGG + chr5_1:56577405-56577424 None:intergenic 25.0%
! ATTGAAAACAACTTTGCAAT+TGG + chr5_1:56577480-56577499 None:intergenic 25.0%
! GAGAATTTAGTATTTACACT+AGG - chr5_1:56577768-56577787 Msa0738720:intron 25.0%
! TTTGTATATGCAAAGTTCTT+TGG - chr5_1:56577878-56577897 Msa0738720:intron 25.0%
!!! TTTTGAATCTAAGACATATG+AGG + chr5_1:56577955-56577974 None:intergenic 25.0%
! ACTGAATGTTCTATTGAAAT+GGG + chr5_1:56578099-56578118 None:intergenic 25.0%
! ACATGTTACATAAAACTGTA+AGG + chr5_1:56578184-56578203 None:intergenic 25.0%
!! TTTCTAAAGATTAGATACTC+TGG - chr5_1:56578340-56578359 Msa0738720:five_prime_UTR 25.0%
! CGAAAAAGAGAAAACAAATT+CGG + chr5_1:56576173-56576192 None:intergenic 25.0%
! AAATCGAATAGAGAATTTAG+GGG + chr5_1:56576401-56576420 None:intergenic 25.0%
! GTAAATCGAATAGAGAATTT+AGG + chr5_1:56576403-56576422 None:intergenic 25.0%
!!! AATGAAATATTTTGGTGGAA+TGG - chr5_1:56576683-56576702 Msa0738720:intron 25.0%
! TGAATTTCTGATGTAAATGT+TGG - chr5_1:56576712-56576731 Msa0738720:intron 25.0%
! GAAACAATTTGATTGAATCA+AGG - chr5_1:56576741-56576760 Msa0738720:intron 25.0%
!!! AATCAAGGATATTTTGAAGT+GGG - chr5_1:56576756-56576775 Msa0738720:intron 25.0%
!! GGTTTTTGTAAGATTTACAA+TGG - chr5_1:56576825-56576844 Msa0738720:intron 25.0%
! TTGAGATCTTAATATGAATC+AGG - chr5_1:56577079-56577098 Msa0738720:intron 25.0%
! TGAGATCTTAATATGAATCA+GGG - chr5_1:56577080-56577099 Msa0738720:intron 25.0%
! ACAAGTAAATCCATTATGTT+AGG + chr5_1:56577153-56577172 None:intergenic 25.0%
! TGTTCTTAGTTATGATTGAT+TGG - chr5_1:56577247-56577266 Msa0738720:intron 25.0%
! CAAATTAGAAACTTCGAAAT+AGG + chr5_1:56577405-56577424 None:intergenic 25.0%
! ATTGAAAACAACTTTGCAAT+TGG + chr5_1:56577480-56577499 None:intergenic 25.0%
! GAGAATTTAGTATTTACACT+AGG - chr5_1:56577768-56577787 Msa0738720:intron 25.0%
! TTTGTATATGCAAAGTTCTT+TGG - chr5_1:56577878-56577897 Msa0738720:intron 25.0%
!!! TTTTGAATCTAAGACATATG+AGG + chr5_1:56577955-56577974 None:intergenic 25.0%
! ACTGAATGTTCTATTGAAAT+GGG + chr5_1:56578099-56578118 None:intergenic 25.0%
! ACATGTTACATAAAACTGTA+AGG + chr5_1:56578184-56578203 None:intergenic 25.0%
!! TTTCTAAAGATTAGATACTC+TGG - chr5_1:56578340-56578359 Msa0738720:five_prime_UTR 25.0%
AAGAGAAAACAAATTCGGAT+GGG + chr5_1:56576168-56576187 None:intergenic 30.0%
AAAGAGAAAACAAATTCGGA+TGG + chr5_1:56576169-56576188 None:intergenic 30.0%
TTGAAATTAGGAGAATGAGA+AGG + chr5_1:56576244-56576263 None:intergenic 30.0%
!! TTGTTTTGTAGAATTCTCCA+AGG - chr5_1:56576471-56576490 Msa0738720:three_prime_UTR 30.0%
!! GAATCAAGGATATTTTGAAG+TGG - chr5_1:56576755-56576774 Msa0738720:intron 30.0%
TTGGTTGTATTTATGCATAG+TGG - chr5_1:56576787-56576806 Msa0738720:intron 30.0%
TGGTTGTATTTATGCATAGT+GGG - chr5_1:56576788-56576807 Msa0738720:intron 30.0%
! TTAGTATTGTGTTACTCATG+TGG - chr5_1:56576883-56576902 Msa0738720:intron 30.0%
TTTAATGTGATCACATGTGT+TGG - chr5_1:56576998-56577017 Msa0738720:intron 30.0%
GAGATCTTAATATGAATCAG+GGG - chr5_1:56577081-56577100 Msa0738720:intron 30.0%
! GATTTTCATGCCTAACATAA+TGG - chr5_1:56577140-56577159 Msa0738720:intron 30.0%
! TTGAATGTAATCAGAAGTGT+CGG + chr5_1:56577595-56577614 None:intergenic 30.0%
CATAGGTAGTAAAAACAGAT+AGG + chr5_1:56577709-56577728 None:intergenic 30.0%
! CTTATGTCATAGGATGTTTT+TGG - chr5_1:56577800-56577819 Msa0738720:intron 30.0%
!! TAACTCTTGATTATTTCTGC+AGG - chr5_1:56578016-56578035 Msa0738720:CDS 30.0%
CACTGAATGTTCTATTGAAA+TGG + chr5_1:56578100-56578119 None:intergenic 30.0%
AGGAGGAAAAAGTTTAGATA+AGG - chr5_1:56578249-56578268 Msa0738720:five_prime_UTR 30.0%
AAGAGAAAACAAATTCGGAT+GGG + chr5_1:56576168-56576187 None:intergenic 30.0%
AAAGAGAAAACAAATTCGGA+TGG + chr5_1:56576169-56576188 None:intergenic 30.0%
TTGAAATTAGGAGAATGAGA+AGG + chr5_1:56576244-56576263 None:intergenic 30.0%
!! TTGTTTTGTAGAATTCTCCA+AGG - chr5_1:56576471-56576490 Msa0738720:three_prime_UTR 30.0%
!! GAATCAAGGATATTTTGAAG+TGG - chr5_1:56576755-56576774 Msa0738720:intron 30.0%
TTGGTTGTATTTATGCATAG+TGG - chr5_1:56576787-56576806 Msa0738720:intron 30.0%
TGGTTGTATTTATGCATAGT+GGG - chr5_1:56576788-56576807 Msa0738720:intron 30.0%
! TTAGTATTGTGTTACTCATG+TGG - chr5_1:56576883-56576902 Msa0738720:intron 30.0%
TTTAATGTGATCACATGTGT+TGG - chr5_1:56576998-56577017 Msa0738720:intron 30.0%
GAGATCTTAATATGAATCAG+GGG - chr5_1:56577081-56577100 Msa0738720:intron 30.0%
! GATTTTCATGCCTAACATAA+TGG - chr5_1:56577140-56577159 Msa0738720:intron 30.0%
! TTGAATGTAATCAGAAGTGT+CGG + chr5_1:56577595-56577614 None:intergenic 30.0%
CATAGGTAGTAAAAACAGAT+AGG + chr5_1:56577709-56577728 None:intergenic 30.0%
! CTTATGTCATAGGATGTTTT+TGG - chr5_1:56577800-56577819 Msa0738720:intron 30.0%
!! TAACTCTTGATTATTTCTGC+AGG - chr5_1:56578016-56578035 Msa0738720:CDS 30.0%
CACTGAATGTTCTATTGAAA+TGG + chr5_1:56578100-56578119 None:intergenic 30.0%
AGGAGGAAAAAGTTTAGATA+AGG - chr5_1:56578249-56578268 Msa0738720:five_prime_UTR 30.0%
! GAGAATGAGAAGGTTTTTGT+TGG + chr5_1:56576234-56576253 None:intergenic 35.0%
ATTCTCAATTCACGCGAAAA+TGG - chr5_1:56576279-56576298 Msa0738720:three_prime_UTR 35.0%
! TTTGAAGTGGGTATTCTGTT+TGG - chr5_1:56576768-56576787 Msa0738720:intron 35.0%
!!! AATCAGGGGATTTTTGAAGT+GGG - chr5_1:56577095-56577114 Msa0738720:intron 35.0%
!! ATTGGTGATTTTCTCTGCAT+TGG - chr5_1:56577265-56577284 Msa0738720:intron 35.0%
ACTGTCAATTTGTGATGCTA+CGG + chr5_1:56577343-56577362 None:intergenic 35.0%
ATGAACAACTCAAACTGACA+TGG + chr5_1:56577646-56577665 None:intergenic 35.0%
GATTGTTCTTTCCATTGAGT+TGG - chr5_1:56577900-56577919 Msa0738720:intron 35.0%
!! TGATTATTTCTGCAGGATGA+CGG - chr5_1:56578023-56578042 Msa0738720:CDS 35.0%
AGATACTCTGGTGAGTAAAT+AGG - chr5_1:56578352-56578371 Msa0738720:five_prime_UTR 35.0%
! GAGAATGAGAAGGTTTTTGT+TGG + chr5_1:56576234-56576253 None:intergenic 35.0%
ATTCTCAATTCACGCGAAAA+TGG - chr5_1:56576279-56576298 Msa0738720:three_prime_UTR 35.0%
! TTTGAAGTGGGTATTCTGTT+TGG - chr5_1:56576768-56576787 Msa0738720:intron 35.0%
!!! AATCAGGGGATTTTTGAAGT+GGG - chr5_1:56577095-56577114 Msa0738720:intron 35.0%
!! ATTGGTGATTTTCTCTGCAT+TGG - chr5_1:56577265-56577284 Msa0738720:intron 35.0%
ACTGTCAATTTGTGATGCTA+CGG + chr5_1:56577343-56577362 None:intergenic 35.0%
ATGAACAACTCAAACTGACA+TGG + chr5_1:56577646-56577665 None:intergenic 35.0%
GATTGTTCTTTCCATTGAGT+TGG - chr5_1:56577900-56577919 Msa0738720:intron 35.0%
!! TGATTATTTCTGCAGGATGA+CGG - chr5_1:56578023-56578042 Msa0738720:CDS 35.0%
AGATACTCTGGTGAGTAAAT+AGG - chr5_1:56578352-56578371 Msa0738720:five_prime_UTR 35.0%
!! GAGAAGGTTTTTGTTGGTGA+AGG + chr5_1:56576228-56576247 None:intergenic 40.0%
CATCACAACAAAACCAATCG+CGG + chr5_1:56576509-56576528 None:intergenic 40.0%
!!! GCGATTGGTTTTGTTGTGAT+GGG - chr5_1:56576508-56576527 Msa0738720:intron 40.0%
! TTTGTTGTGATGGGATTCGT+TGG - chr5_1:56576517-56576536 Msa0738720:intron 40.0%
! TTGTTGTGATGGGATTCGTT+GGG - chr5_1:56576518-56576537 Msa0738720:intron 40.0%
CCAACGATGATGTTGTTGAT+TGG + chr5_1:56576568-56576587 None:intergenic 40.0%
CCAATCAACAACATCATCGT+TGG - chr5_1:56576565-56576584 Msa0738720:intron 40.0%
AACAACATCATCGTTGGATC+TGG - chr5_1:56576571-56576590 Msa0738720:intron 40.0%
!!! GCATAGTGGGTTTTAGTTGT+TGG - chr5_1:56576801-56576820 Msa0738720:intron 40.0%
!!! TAGTGGGTTTTAGTTGTTGG+AGG - chr5_1:56576804-56576823 Msa0738720:intron 40.0%
! TGCATGTTTGCTACAATGCA+TGG - chr5_1:56576858-56576877 Msa0738720:intron 40.0%
!! TCCGTTAGAAGTGTTAGTGT+CGG - chr5_1:56577036-56577055 Msa0738720:intron 40.0%
ACCGACACTAACACTTCTAA+CGG + chr5_1:56577040-56577059 None:intergenic 40.0%
!! GAATCAGGGGATTTTTGAAG+TGG - chr5_1:56577094-56577113 Msa0738720:intron 40.0%
! TTTGAAGTGGGTGATCTGTT+TGG - chr5_1:56577107-56577126 Msa0738720:intron 40.0%
!!! GATTTTCTCTGCATTGGTTG+TGG - chr5_1:56577271-56577290 Msa0738720:intron 40.0%
ACACAAGACACAACACTGAA+TGG + chr5_1:56577623-56577642 None:intergenic 40.0%
CTTCACAGAAATAGTTCCAC+GGG + chr5_1:56577742-56577761 None:intergenic 40.0%
GCTTCACAGAAATAGTTCCA+CGG + chr5_1:56577743-56577762 None:intergenic 40.0%
!! GTGGAACTATTTCTGTGAAG+CGG - chr5_1:56577742-56577761 Msa0738720:intron 40.0%
GCTGTAGCAACTTATGTCAT+AGG - chr5_1:56577790-56577809 Msa0738720:intron 40.0%
AATCACAGCAACCAACTCAA+TGG + chr5_1:56577914-56577933 None:intergenic 40.0%
!! GATTATTTCTGCAGGATGAC+GGG - chr5_1:56578024-56578043 Msa0738720:CDS 40.0%
AGGTCAAAAGTCTCAACACA+TGG + chr5_1:56578221-56578240 None:intergenic 40.0%
!! GAGAAGGTTTTTGTTGGTGA+AGG + chr5_1:56576228-56576247 None:intergenic 40.0%
CATCACAACAAAACCAATCG+CGG + chr5_1:56576509-56576528 None:intergenic 40.0%
!!! GCGATTGGTTTTGTTGTGAT+GGG - chr5_1:56576508-56576527 Msa0738720:intron 40.0%
! TTTGTTGTGATGGGATTCGT+TGG - chr5_1:56576517-56576536 Msa0738720:intron 40.0%
! TTGTTGTGATGGGATTCGTT+GGG - chr5_1:56576518-56576537 Msa0738720:intron 40.0%
CCAACGATGATGTTGTTGAT+TGG + chr5_1:56576568-56576587 None:intergenic 40.0%
CCAATCAACAACATCATCGT+TGG - chr5_1:56576565-56576584 Msa0738720:intron 40.0%
AACAACATCATCGTTGGATC+TGG - chr5_1:56576571-56576590 Msa0738720:intron 40.0%
!!! GCATAGTGGGTTTTAGTTGT+TGG - chr5_1:56576801-56576820 Msa0738720:intron 40.0%
!!! TAGTGGGTTTTAGTTGTTGG+AGG - chr5_1:56576804-56576823 Msa0738720:intron 40.0%
! TGCATGTTTGCTACAATGCA+TGG - chr5_1:56576858-56576877 Msa0738720:intron 40.0%
!! TCCGTTAGAAGTGTTAGTGT+CGG - chr5_1:56577036-56577055 Msa0738720:intron 40.0%
ACCGACACTAACACTTCTAA+CGG + chr5_1:56577040-56577059 None:intergenic 40.0%
!! GAATCAGGGGATTTTTGAAG+TGG - chr5_1:56577094-56577113 Msa0738720:intron 40.0%
! TTTGAAGTGGGTGATCTGTT+TGG - chr5_1:56577107-56577126 Msa0738720:intron 40.0%
!!! GATTTTCTCTGCATTGGTTG+TGG - chr5_1:56577271-56577290 Msa0738720:intron 40.0%
ACACAAGACACAACACTGAA+TGG + chr5_1:56577623-56577642 None:intergenic 40.0%
CTTCACAGAAATAGTTCCAC+GGG + chr5_1:56577742-56577761 None:intergenic 40.0%
GCTTCACAGAAATAGTTCCA+CGG + chr5_1:56577743-56577762 None:intergenic 40.0%
!! GTGGAACTATTTCTGTGAAG+CGG - chr5_1:56577742-56577761 Msa0738720:intron 40.0%
GCTGTAGCAACTTATGTCAT+AGG - chr5_1:56577790-56577809 Msa0738720:intron 40.0%
AATCACAGCAACCAACTCAA+TGG + chr5_1:56577914-56577933 None:intergenic 40.0%
!! GATTATTTCTGCAGGATGAC+GGG - chr5_1:56578024-56578043 Msa0738720:CDS 40.0%
AGGTCAAAAGTCTCAACACA+TGG + chr5_1:56578221-56578240 None:intergenic 40.0%
GGTGAAGGAAGCAGATGAAT+TGG + chr5_1:56576213-56576232 None:intergenic 45.0%
TGGATCGAAGACGGAATCAA+TGG + chr5_1:56576308-56576327 None:intergenic 45.0%
TTCTCTGAGTGGATCGAAGA+CGG + chr5_1:56576317-56576336 None:intergenic 45.0%
TCTTTGGCGAATTCTCTGAG+TGG + chr5_1:56576328-56576347 None:intergenic 45.0%
AATTCGCCAAAGACAGCGTT+AGG - chr5_1:56576335-56576354 Msa0738720:three_prime_UTR 45.0%
AGGGGTTATACCTTTGCGAT+CGG + chr5_1:56576383-56576402 None:intergenic 45.0%
!!! CGCGATTGGTTTTGTTGTGA+TGG - chr5_1:56576507-56576526 Msa0738720:intron 45.0%
CATCATCGTTGGATCTGGTT+AGG - chr5_1:56576576-56576595 Msa0738720:intron 45.0%
CTTCTAACGGAAGATGTGTC+TGG + chr5_1:56577027-56577046 None:intergenic 45.0%
! GACTTTTGACCTGTTGCTCA+AGG - chr5_1:56578229-56578248 Msa0738720:exon 45.0%
! TTTTGACCTGTTGCTCAAGG+AGG - chr5_1:56578232-56578251 Msa0738720:exon 45.0%
! CTTTTTCCTCCTTGAGCAAC+AGG + chr5_1:56578241-56578260 None:intergenic 45.0%
GGTGAAGGAAGCAGATGAAT+TGG + chr5_1:56576213-56576232 None:intergenic 45.0%
TGGATCGAAGACGGAATCAA+TGG + chr5_1:56576308-56576327 None:intergenic 45.0%
TTCTCTGAGTGGATCGAAGA+CGG + chr5_1:56576317-56576336 None:intergenic 45.0%
TCTTTGGCGAATTCTCTGAG+TGG + chr5_1:56576328-56576347 None:intergenic 45.0%
AATTCGCCAAAGACAGCGTT+AGG - chr5_1:56576335-56576354 Msa0738720:three_prime_UTR 45.0%
AGGGGTTATACCTTTGCGAT+CGG + chr5_1:56576383-56576402 None:intergenic 45.0%
!!! CGCGATTGGTTTTGTTGTGA+TGG - chr5_1:56576507-56576526 Msa0738720:intron 45.0%
CATCATCGTTGGATCTGGTT+AGG - chr5_1:56576576-56576595 Msa0738720:intron 45.0%
CTTCTAACGGAAGATGTGTC+TGG + chr5_1:56577027-56577046 None:intergenic 45.0%
! GACTTTTGACCTGTTGCTCA+AGG - chr5_1:56578229-56578248 Msa0738720:exon 45.0%
! TTTTGACCTGTTGCTCAAGG+AGG - chr5_1:56578232-56578251 Msa0738720:exon 45.0%
! CTTTTTCCTCCTTGAGCAAC+AGG + chr5_1:56578241-56578260 None:intergenic 45.0%
AACGAGCCTAACGCTGTCTT+TGG + chr5_1:56576344-56576363 None:intergenic 50.0%
TACCTTTGCGATCGGGTTTG+TGG + chr5_1:56576375-56576394 None:intergenic 50.0%
GGGGTTATACCTTTGCGATC+GGG + chr5_1:56576382-56576401 None:intergenic 50.0%
ATAGTTCCACGGGCTCTTGA+GGG + chr5_1:56577732-56577751 None:intergenic 50.0%
AATAGTTCCACGGGCTCTTG+AGG + chr5_1:56577733-56577752 None:intergenic 50.0%
TGCTGTGATTGATTGCCGAG+CGG - chr5_1:56577924-56577943 Msa0738720:intron 50.0%
ACATATGAGGCAGTTCCGCT+CGG + chr5_1:56577942-56577961 None:intergenic 50.0%
ACGGGAATTACAATCGCCTC+AGG - chr5_1:56578042-56578061 Msa0738720:CDS 50.0%
CCTGCTTCACAACTTTCCTG+AGG + chr5_1:56578061-56578080 None:intergenic 50.0%
CCTCAGGAAAGTTGTGAAGC+AGG - chr5_1:56578058-56578077 Msa0738720:intron 50.0%
CAGGAAAGTTGTGAAGCAGG+AGG - chr5_1:56578061-56578080 Msa0738720:intron 50.0%
GAAAGTTGTGAAGCAGGAGG+AGG - chr5_1:56578064-56578083 Msa0738720:intron 50.0%
AACGAGCCTAACGCTGTCTT+TGG + chr5_1:56576344-56576363 None:intergenic 50.0%
TACCTTTGCGATCGGGTTTG+TGG + chr5_1:56576375-56576394 None:intergenic 50.0%
GGGGTTATACCTTTGCGATC+GGG + chr5_1:56576382-56576401 None:intergenic 50.0%
ATAGTTCCACGGGCTCTTGA+GGG + chr5_1:56577732-56577751 None:intergenic 50.0%
AATAGTTCCACGGGCTCTTG+AGG + chr5_1:56577733-56577752 None:intergenic 50.0%
TGCTGTGATTGATTGCCGAG+CGG - chr5_1:56577924-56577943 Msa0738720:intron 50.0%
ACATATGAGGCAGTTCCGCT+CGG + chr5_1:56577942-56577961 None:intergenic 50.0%
ACGGGAATTACAATCGCCTC+AGG - chr5_1:56578042-56578061 Msa0738720:CDS 50.0%
CCTGCTTCACAACTTTCCTG+AGG + chr5_1:56578061-56578080 None:intergenic 50.0%
CCTCAGGAAAGTTGTGAAGC+AGG - chr5_1:56578058-56578077 Msa0738720:intron 50.0%
CAGGAAAGTTGTGAAGCAGG+AGG - chr5_1:56578061-56578080 Msa0738720:intron 50.0%
GAAAGTTGTGAAGCAGGAGG+AGG - chr5_1:56578064-56578083 Msa0738720:intron 50.0%
TGCCACAAACCCGATCGCAA+AGG - chr5_1:56576370-56576389 Msa0738720:three_prime_UTR 55.0%
! GTTGCTGTTCGTACCGCGAT+TGG - chr5_1:56576493-56576512 Msa0738720:intron 55.0%
TGCCACAAACCCGATCGCAA+AGG - chr5_1:56576370-56576389 Msa0738720:three_prime_UTR 55.0%
! GTTGCTGTTCGTACCGCGAT+TGG - chr5_1:56576493-56576512 Msa0738720:intron 55.0%
CGCGGTACGAACAGCAACCT+TGG + chr5_1:56576491-56576510 None:intergenic 60.0%
CGCGGTACGAACAGCAACCT+TGG + chr5_1:56576491-56576510 None:intergenic 60.0%
! CCACGGGCTCTTGAGGGCAT+AGG + chr5_1:56577726-56577745 None:intergenic 65.0%
CCTATGCCCTCAAGAGCCCG+TGG - chr5_1:56577723-56577742 Msa0738720:intron 65.0%
! CCACGGGCTCTTGAGGGCAT+AGG + chr5_1:56577726-56577745 None:intergenic 65.0%
CCTATGCCCTCAAGAGCCCG+TGG - chr5_1:56577723-56577742 Msa0738720:intron 65.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 56576164 56578380 56576164 ID=Msa0738720;Name=Msa0738720
chr5_1 mRNA 56576164 56578380 56576164 ID=Msa0738720-mRNA-1;Parent=Msa0738720;Name=Msa0738720-mRNA-1;_AED=0.06;_eAED=0.06;_QI=134|1|1|1|0.5|1|3|343|69
chr5_1 exon 56576164 56576506 56576164 ID=Msa0738720-mRNA-1:exon:16058;Parent=Msa0738720-mRNA-1
chr5_1 exon 56577947 56578062 56577947 ID=Msa0738720-mRNA-1:exon:16057;Parent=Msa0738720-mRNA-1
chr5_1 exon 56578153 56578380 56578153 ID=Msa0738720-mRNA-1:exon:16056;Parent=Msa0738720-mRNA-1
chr5_1 five_prime_UTR 56578247 56578380 56578247 ID=Msa0738720-mRNA-1:five_prime_utr;Parent=Msa0738720-mRNA-1
chr5_1 CDS 56578153 56578246 56578153 ID=Msa0738720-mRNA-1:cds;Parent=Msa0738720-mRNA-1
chr5_1 CDS 56577947 56578062 56577947 ID=Msa0738720-mRNA-1:cds;Parent=Msa0738720-mRNA-1
chr5_1 three_prime_UTR 56576164 56576506 56576164 ID=Msa0738720-mRNA-1:three_prime_utr;Parent=Msa0738720-mRNA-1
Gene Sequence

>Msa0738720

ATGGACGCCATTGATTCCGTCTTCGATCCACTCAGAGAATTCGCCAAAGACAGCGTTAGGCTCGTTAAGCGTTGCCACAAACCCGATCGCAAAGAATTCTCCAAGGTTGCTGTTCGTACCGCGATTGGTTTTGTTGTGATGGGATTCGTTGGGTTTTTCGTTAAGCTTATCTTTATTCCAATCAACAACATCATCGTTGGATCTGGTTAG
Protein sequence

>Msa0738720

MDAIDSVFDPLREFAKDSVRLVKRCHKPDRKEFSKVAVRTAIGFVVMGFVGFFVKLIFIPINNIIVGSG*