AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0859320

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0859320 MtrunA17_Chr5g0438181 100.000 69 0 0 1 69 1 69 1.09e-44 139
Msa0859320 MtrunA17_Chr4g0071611 100.000 69 0 0 1 69 1 69 1.72e-44 139
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0859320 AT4G24920.1 92.647 68 5 0 1 68 1 68 4.96e-41 128
Msa0859320 AT5G50460.1 92.647 68 5 0 1 68 1 68 4.96e-41 128
Msa0859320 AT3G48570.1 86.957 69 9 0 1 69 1 69 2.17e-39 124

Find 42 sgRNAs with CRISPR-Local

Find 230 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTCTCAATTCACGCGAAAA+TGG 0.229980 5_4:-57667933 Msa0859320:exon
TGAGAAATGAAATTGAAATT+AGG 0.298155 5_4:+57667964 None:intergenic
CACTGAATGTTCTATTGAAA+TGG 0.316272 5_4:+57666101 None:intergenic
AACGAGCCTAACGCTGTCTT+TGG 0.352610 5_4:+57667871 None:intergenic
TGAAATTATCAATATTAGAT+TGG 0.362513 5_4:-57665831 Msa0859320:three_prime_UTR
TTATCTATATTTCTTATCTT+CGG 0.410507 5_4:-57666047 Msa0859320:three_prime_UTR
TACCTTTGCGATCGGGTTTG+TGG 0.417825 5_4:+57667840 None:intergenic
CCAACGATGATGTTGTTGAT+TGG 0.436566 5_4:+57667647 None:intergenic
ACATGTTACATAAAACTGTA+AGG 0.442953 5_4:+57666017 None:intergenic
CATCATCGTTGGATCTGGTT+AGG 0.451612 5_4:-57667636 Msa0859320:intron
GGGGTTATACCTTTGCGATC+GGG 0.456406 5_4:+57667833 None:intergenic
AACAACATCATCGTTGGATC+TGG 0.457089 5_4:-57667641 Msa0859320:CDS
GACTCAGGTGAGGGAGTGAT+AGG 0.460590 5_4:-57665802 Msa0859320:three_prime_UTR
TGGATCGAAGACGGAATCAA+TGG 0.467824 5_4:+57667907 None:intergenic
AGGGGTTATACCTTTGCGAT+CGG 0.473227 5_4:+57667832 None:intergenic
GATTATTTCTGCAGGATGAC+GGG 0.476757 5_4:-57666175 Msa0859320:intron
TTTGTTGTGATGGGATTCGT+TGG 0.478676 5_4:-57667695 Msa0859320:CDS
GTTGCTGTTCGTACCGCGAT+TGG 0.481738 5_4:-57667719 Msa0859320:CDS
GGTGAAGGAAGCAGATGAAT+TGG 0.489333 5_4:+57668007 None:intergenic
CGCGGTACGAACAGCAACCT+TGG 0.492229 5_4:+57667724 None:intergenic
TTTCTAAAGATTAGATACTC+TGG 0.509291 5_4:-57665858 Msa0859320:three_prime_UTR
TGATTATTTCTGCAGGATGA+CGG 0.511253 5_4:-57666176 Msa0859320:intron
TTCTCTGAGTGGATCGAAGA+CGG 0.515282 5_4:+57667898 None:intergenic
ACTGAATGTTCTATTGAAAT+GGG 0.522320 5_4:+57666102 None:intergenic
CCTCAGGAAAGTTGTGAAGC+AGG 0.527193 5_4:-57666141 Msa0859320:three_prime_UTR
TTGTTGTGATGGGATTCGTT+GGG 0.527754 5_4:-57667694 Msa0859320:CDS
AAAGAGAAAACAAATTCGGA+TGG 0.529021 5_4:+57668051 None:intergenic
CCAATCAACAACATCATCGT+TGG 0.538152 5_4:-57667647 Msa0859320:CDS
TTAGATTGGCTTAATGACTC+AGG 0.550557 5_4:-57665817 Msa0859320:three_prime_UTR
GAAAGTTGTGAAGCAGGAGG+AGG 0.550624 5_4:-57666135 Msa0859320:three_prime_UTR
ACGGGAATTACAATCGCCTC+AGG 0.556837 5_4:-57666157 Msa0859320:three_prime_UTR
AATTCGCCAAAGACAGCGTT+AGG 0.569841 5_4:-57667877 Msa0859320:CDS
TTGAAATTAGGAGAATGAGA+AGG 0.584098 5_4:+57667976 None:intergenic
AAGAGAAAACAAATTCGGAT+GGG 0.611419 5_4:+57668052 None:intergenic
TGGCTTAATGACTCAGGTGA+GGG 0.618608 5_4:-57665811 Msa0859320:three_prime_UTR
CCTGCTTCACAACTTTCCTG+AGG 0.626602 5_4:+57666141 None:intergenic
TTGGCTTAATGACTCAGGTG+AGG 0.642170 5_4:-57665812 Msa0859320:three_prime_UTR
TGCCACAAACCCGATCGCAA+AGG 0.648616 5_4:-57667842 Msa0859320:intron
AGGTCAAAAGTCTCAACACA+TGG 0.662789 5_4:+57665980 None:intergenic
CAGGAAAGTTGTGAAGCAGG+AGG 0.667526 5_4:-57666138 Msa0859320:three_prime_UTR
CATCACAACAAAACCAATCG+CGG 0.679795 5_4:+57667706 None:intergenic
TCTTTGGCGAATTCTCTGAG+TGG 0.681670 5_4:+57667887 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTGAAAAGAAAAAAAAA+TGG - chr5_4:57666593-57666612 Msa0859320:intron 10.0%
!!! ATTTTGAAAAGAAAAAAAAA+TGG - chr5_4:57666593-57666612 Msa0859320:intron 10.0%
!!! TTGATTTGAATGAAATATTT+TGG - chr5_4:57666300-57666319 Msa0859320:intron 15.0%
!!! TTTTATAATGTTCATCTAAA+TGG - chr5_4:57666922-57666941 Msa0859320:intron 15.0%
!! ACAAAATAAACATTATATTG+AGG + chr5_4:57667474-57667493 None:intergenic 15.0%
!!! ATAATGTTTATTTTGTCTTT+TGG - chr5_4:57667478-57667497 Msa0859320:intron 15.0%
!! TTATCTATATTTCTTATCTT+CGG - chr5_4:57667791-57667810 Msa0859320:intron 15.0%
!! TGAAATTATCAATATTAGAT+TGG - chr5_4:57668007-57668026 Msa0859320:five_prime_UTR 15.0%
!!! TTGATTTGAATGAAATATTT+TGG - chr5_4:57666300-57666319 Msa0859320:intron 15.0%
!!! TTTTATAATGTTCATCTAAA+TGG - chr5_4:57666922-57666941 Msa0859320:intron 15.0%
!! ACAAAATAAACATTATATTG+AGG + chr5_4:57667474-57667493 None:intergenic 15.0%
!!! ATAATGTTTATTTTGTCTTT+TGG - chr5_4:57667478-57667497 Msa0859320:intron 15.0%
!! TTATCTATATTTCTTATCTT+CGG - chr5_4:57667791-57667810 Msa0859320:intron 15.0%
!! TGAAATTATCAATATTAGAT+TGG - chr5_4:57668007-57668026 Msa0859320:five_prime_UTR 15.0%
!! TGAGAAATGAAATTGAAATT+AGG + chr5_4:57665877-57665896 None:intergenic 20.0%
!! TAAATCGAATAGAGAATTTA+GGG + chr5_4:57666028-57666047 None:intergenic 20.0%
!! TGATTTAAATCTCTATATTG+TGG - chr5_4:57666050-57666069 Msa0859320:three_prime_UTR 20.0%
!!! ATTTGAATGAAATATTTTGG+TGG - chr5_4:57666303-57666322 Msa0859320:intron 20.0%
!!! ATTTTTTGTATGATTTGCAA+TGG - chr5_4:57666800-57666819 Msa0859320:intron 20.0%
!! TAAACATATTTGTGACAATA+AGG - chr5_4:57667057-57667076 Msa0859320:intron 20.0%
!! AAACATATTTGTGACAATAA+GGG - chr5_4:57667058-57667077 Msa0859320:intron 20.0%
!! TGAGAAATGAAATTGAAATT+AGG + chr5_4:57665877-57665896 None:intergenic 20.0%
!! TAAATCGAATAGAGAATTTA+GGG + chr5_4:57666028-57666047 None:intergenic 20.0%
!! TGATTTAAATCTCTATATTG+TGG - chr5_4:57666050-57666069 Msa0859320:three_prime_UTR 20.0%
!!! ATTTGAATGAAATATTTTGG+TGG - chr5_4:57666303-57666322 Msa0859320:intron 20.0%
!!! ATTTTTTGTATGATTTGCAA+TGG - chr5_4:57666800-57666819 Msa0859320:intron 20.0%
!! TAAACATATTTGTGACAATA+AGG - chr5_4:57667057-57667076 Msa0859320:intron 20.0%
!! AAACATATTTGTGACAATAA+GGG - chr5_4:57667058-57667077 Msa0859320:intron 20.0%
! CGAAAAAGAGAAAACAAATT+CGG + chr5_4:57665794-57665813 None:intergenic 25.0%
! AAATCGAATAGAGAATTTAG+GGG + chr5_4:57666027-57666046 None:intergenic 25.0%
! GTAAATCGAATAGAGAATTT+AGG + chr5_4:57666029-57666048 None:intergenic 25.0%
!!! AATGAAATATTTTGGTGGAA+TGG - chr5_4:57666308-57666327 Msa0859320:intron 25.0%
! TGAATTTCTGATGTAAATGT+TGG - chr5_4:57666337-57666356 Msa0859320:intron 25.0%
! GAAACAATTTGATTGAATCA+AGG - chr5_4:57666366-57666385 Msa0859320:intron 25.0%
!!! AATCAAGGATATTTTGAAGT+GGG - chr5_4:57666381-57666400 Msa0859320:intron 25.0%
!! GGTTTTTGTAAGATTTACAA+TGG - chr5_4:57666450-57666469 Msa0859320:intron 25.0%
! TTGAGATCTTAATATGAATC+AGG - chr5_4:57666702-57666721 Msa0859320:intron 25.0%
! TGAGATCTTAATATGAATCA+GGG - chr5_4:57666703-57666722 Msa0859320:intron 25.0%
! ACAAGTAAATCCATTATGTT+AGG + chr5_4:57666776-57666795 None:intergenic 25.0%
! ATTGAAAACAACTTTGCAAT+TGG + chr5_4:57667108-57667127 None:intergenic 25.0%
! GAGAATTTAGTATTTACACT+AGG - chr5_4:57667404-57667423 Msa0859320:intron 25.0%
! ACTGAATGTTCTATTGAAAT+GGG + chr5_4:57667739-57667758 None:intergenic 25.0%
! ACATGTTACATAAAACTGTA+AGG + chr5_4:57667824-57667843 None:intergenic 25.0%
!! TTTCTAAAGATTAGATACTC+TGG - chr5_4:57667980-57667999 Msa0859320:five_prime_UTR 25.0%
! CGAAAAAGAGAAAACAAATT+CGG + chr5_4:57665794-57665813 None:intergenic 25.0%
! AAATCGAATAGAGAATTTAG+GGG + chr5_4:57666027-57666046 None:intergenic 25.0%
! GTAAATCGAATAGAGAATTT+AGG + chr5_4:57666029-57666048 None:intergenic 25.0%
!!! AATGAAATATTTTGGTGGAA+TGG - chr5_4:57666308-57666327 Msa0859320:intron 25.0%
! TGAATTTCTGATGTAAATGT+TGG - chr5_4:57666337-57666356 Msa0859320:intron 25.0%
! GAAACAATTTGATTGAATCA+AGG - chr5_4:57666366-57666385 Msa0859320:intron 25.0%
!!! AATCAAGGATATTTTGAAGT+GGG - chr5_4:57666381-57666400 Msa0859320:intron 25.0%
!! GGTTTTTGTAAGATTTACAA+TGG - chr5_4:57666450-57666469 Msa0859320:intron 25.0%
! TTGAGATCTTAATATGAATC+AGG - chr5_4:57666702-57666721 Msa0859320:intron 25.0%
! TGAGATCTTAATATGAATCA+GGG - chr5_4:57666703-57666722 Msa0859320:intron 25.0%
! ACAAGTAAATCCATTATGTT+AGG + chr5_4:57666776-57666795 None:intergenic 25.0%
! ATTGAAAACAACTTTGCAAT+TGG + chr5_4:57667108-57667127 None:intergenic 25.0%
! GAGAATTTAGTATTTACACT+AGG - chr5_4:57667404-57667423 Msa0859320:intron 25.0%
! ACTGAATGTTCTATTGAAAT+GGG + chr5_4:57667739-57667758 None:intergenic 25.0%
! ACATGTTACATAAAACTGTA+AGG + chr5_4:57667824-57667843 None:intergenic 25.0%
!! TTTCTAAAGATTAGATACTC+TGG - chr5_4:57667980-57667999 Msa0859320:five_prime_UTR 25.0%
AAGAGAAAACAAATTCGGAT+GGG + chr5_4:57665789-57665808 None:intergenic 30.0%
AAAGAGAAAACAAATTCGGA+TGG + chr5_4:57665790-57665809 None:intergenic 30.0%
TTGAAATTAGGAGAATGAGA+AGG + chr5_4:57665865-57665884 None:intergenic 30.0%
!! TTGTTTTGTAGAATTCTCCA+AGG - chr5_4:57666097-57666116 Msa0859320:three_prime_UTR 30.0%
!! GAATCAAGGATATTTTGAAG+TGG - chr5_4:57666380-57666399 Msa0859320:intron 30.0%
TTGGTTGTATTTATGCATAG+TGG - chr5_4:57666412-57666431 Msa0859320:intron 30.0%
TGGTTGTATTTATGCATAGT+GGG - chr5_4:57666413-57666432 Msa0859320:intron 30.0%
! TTAGTATTGTGTTACTCATG+TGG - chr5_4:57666508-57666527 Msa0859320:intron 30.0%
TTTAATGTGATCACATGTGT+TGG - chr5_4:57666621-57666640 Msa0859320:intron 30.0%
GAGATCTTAATATGAATCAG+GGG - chr5_4:57666704-57666723 Msa0859320:intron 30.0%
! GATTTTCATGCCTAACATAA+TGG - chr5_4:57666763-57666782 Msa0859320:intron 30.0%
TGTTCGTAGTTATGATTGAT+TGG - chr5_4:57666875-57666894 Msa0859320:intron 30.0%
! TTGAATGTAATCAGAAGTGT+CGG + chr5_4:57667231-57667250 None:intergenic 30.0%
CATAGGTAGTAAAAACAGAT+AGG + chr5_4:57667345-57667364 None:intergenic 30.0%
! CTTATGTCAAAGGATGTTTT+TGG - chr5_4:57667436-57667455 Msa0859320:intron 30.0%
!! TAACTCTTGATTATTTCTGC+AGG - chr5_4:57667655-57667674 Msa0859320:CDS 30.0%
CACTGAATGTTCTATTGAAA+TGG + chr5_4:57667740-57667759 None:intergenic 30.0%
AGGAGGAAAAAGTTTAGATA+AGG - chr5_4:57667889-57667908 Msa0859320:CDS 30.0%
AAGAGAAAACAAATTCGGAT+GGG + chr5_4:57665789-57665808 None:intergenic 30.0%
AAAGAGAAAACAAATTCGGA+TGG + chr5_4:57665790-57665809 None:intergenic 30.0%
TTGAAATTAGGAGAATGAGA+AGG + chr5_4:57665865-57665884 None:intergenic 30.0%
!! TTGTTTTGTAGAATTCTCCA+AGG - chr5_4:57666097-57666116 Msa0859320:three_prime_UTR 30.0%
!! GAATCAAGGATATTTTGAAG+TGG - chr5_4:57666380-57666399 Msa0859320:intron 30.0%
TTGGTTGTATTTATGCATAG+TGG - chr5_4:57666412-57666431 Msa0859320:intron 30.0%
TGGTTGTATTTATGCATAGT+GGG - chr5_4:57666413-57666432 Msa0859320:intron 30.0%
! TTAGTATTGTGTTACTCATG+TGG - chr5_4:57666508-57666527 Msa0859320:intron 30.0%
TTTAATGTGATCACATGTGT+TGG - chr5_4:57666621-57666640 Msa0859320:intron 30.0%
GAGATCTTAATATGAATCAG+GGG - chr5_4:57666704-57666723 Msa0859320:intron 30.0%
! GATTTTCATGCCTAACATAA+TGG - chr5_4:57666763-57666782 Msa0859320:intron 30.0%
TGTTCGTAGTTATGATTGAT+TGG - chr5_4:57666875-57666894 Msa0859320:intron 30.0%
! TTGAATGTAATCAGAAGTGT+CGG + chr5_4:57667231-57667250 None:intergenic 30.0%
CATAGGTAGTAAAAACAGAT+AGG + chr5_4:57667345-57667364 None:intergenic 30.0%
! CTTATGTCAAAGGATGTTTT+TGG - chr5_4:57667436-57667455 Msa0859320:intron 30.0%
!! TAACTCTTGATTATTTCTGC+AGG - chr5_4:57667655-57667674 Msa0859320:CDS 30.0%
CACTGAATGTTCTATTGAAA+TGG + chr5_4:57667740-57667759 None:intergenic 30.0%
AGGAGGAAAAAGTTTAGATA+AGG - chr5_4:57667889-57667908 Msa0859320:CDS 30.0%
! GAGAATGAGAAGGTTTTTGT+TGG + chr5_4:57665855-57665874 None:intergenic 35.0%
ATTCTCAATTCACGCGAAAA+TGG - chr5_4:57665905-57665924 Msa0859320:three_prime_UTR 35.0%
! TTTGAAGTGGGTATTCTGTT+TGG - chr5_4:57666393-57666412 Msa0859320:intron 35.0%
!!! AATCAGGGGATTTTTGAAGT+GGG - chr5_4:57666718-57666737 Msa0859320:intron 35.0%
!! ATTGGTGATTTTCTCTGCAT+TGG - chr5_4:57666893-57666912 Msa0859320:intron 35.0%
ACTGTCAATTTGTGATGCTA+CGG + chr5_4:57666971-57666990 None:intergenic 35.0%
ATGAACAACTCAAACTGACA+TGG + chr5_4:57667282-57667301 None:intergenic 35.0%
GATTGTTCTTTCCATTGAGT+TGG - chr5_4:57667536-57667555 Msa0859320:intron 35.0%
TGAATCCTGTAAGACATATG+AGG + chr5_4:57667591-57667610 None:intergenic 35.0%
!! TGATTATTTCTGCAGGATGA+CGG - chr5_4:57667662-57667681 Msa0859320:CDS 35.0%
!! TTAGATTGGCTTAATGACTC+AGG - chr5_4:57668021-57668040 Msa0859320:five_prime_UTR 35.0%
! GAGAATGAGAAGGTTTTTGT+TGG + chr5_4:57665855-57665874 None:intergenic 35.0%
ATTCTCAATTCACGCGAAAA+TGG - chr5_4:57665905-57665924 Msa0859320:three_prime_UTR 35.0%
! TTTGAAGTGGGTATTCTGTT+TGG - chr5_4:57666393-57666412 Msa0859320:intron 35.0%
!!! AATCAGGGGATTTTTGAAGT+GGG - chr5_4:57666718-57666737 Msa0859320:intron 35.0%
!! ATTGGTGATTTTCTCTGCAT+TGG - chr5_4:57666893-57666912 Msa0859320:intron 35.0%
ACTGTCAATTTGTGATGCTA+CGG + chr5_4:57666971-57666990 None:intergenic 35.0%
ATGAACAACTCAAACTGACA+TGG + chr5_4:57667282-57667301 None:intergenic 35.0%
GATTGTTCTTTCCATTGAGT+TGG - chr5_4:57667536-57667555 Msa0859320:intron 35.0%
TGAATCCTGTAAGACATATG+AGG + chr5_4:57667591-57667610 None:intergenic 35.0%
!! TGATTATTTCTGCAGGATGA+CGG - chr5_4:57667662-57667681 Msa0859320:CDS 35.0%
!! TTAGATTGGCTTAATGACTC+AGG - chr5_4:57668021-57668040 Msa0859320:five_prime_UTR 35.0%
!! GAGAAGGTTTTTGTTGGTGA+AGG + chr5_4:57665849-57665868 None:intergenic 40.0%
CATCACAACAAAACCAATCG+CGG + chr5_4:57666135-57666154 None:intergenic 40.0%
!!! GCGATTGGTTTTGTTGTGAT+GGG - chr5_4:57666134-57666153 Msa0859320:three_prime_UTR 40.0%
! TTTGTTGTGATGGGATTCGT+TGG - chr5_4:57666143-57666162 Msa0859320:three_prime_UTR 40.0%
! TTGTTGTGATGGGATTCGTT+GGG - chr5_4:57666144-57666163 Msa0859320:three_prime_UTR 40.0%
CCAACGATGATGTTGTTGAT+TGG + chr5_4:57666194-57666213 None:intergenic 40.0%
CCAATCAACAACATCATCGT+TGG - chr5_4:57666191-57666210 Msa0859320:intron 40.0%
AACAACATCATCGTTGGATC+TGG - chr5_4:57666197-57666216 Msa0859320:intron 40.0%
!!! GCATAGTGGGTTTTAGTTGT+TGG - chr5_4:57666426-57666445 Msa0859320:intron 40.0%
!!! TAGTGGGTTTTAGTTGTTGG+AGG - chr5_4:57666429-57666448 Msa0859320:intron 40.0%
! TGCATGTTTGCTACAATGCA+TGG - chr5_4:57666483-57666502 Msa0859320:intron 40.0%
!! TCCGTTAGAAGTGTTAGTGT+CGG - chr5_4:57666659-57666678 Msa0859320:intron 40.0%
ACCGACACTAACACTTCTAA+CGG + chr5_4:57666663-57666682 None:intergenic 40.0%
!! GAATCAGGGGATTTTTGAAG+TGG - chr5_4:57666717-57666736 Msa0859320:intron 40.0%
! TTTGAAGTGGGTGATCTGTT+TGG - chr5_4:57666730-57666749 Msa0859320:intron 40.0%
!!! GATTTTCTCTGCATTGGTTG+TGG - chr5_4:57666899-57666918 Msa0859320:intron 40.0%
ACACAAGACACAACACTGAA+TGG + chr5_4:57667259-57667278 None:intergenic 40.0%
CTTCACAGAAATAGTTCCAC+GGG + chr5_4:57667378-57667397 None:intergenic 40.0%
GCTTCACAGAAATAGTTCCA+CGG + chr5_4:57667379-57667398 None:intergenic 40.0%
GCTGTAGCAACTTATGTCAA+AGG - chr5_4:57667426-57667445 Msa0859320:intron 40.0%
AATCACAGCAACCAACTCAA+TGG + chr5_4:57667550-57667569 None:intergenic 40.0%
AACTGCCTCATATGTCTTAC+AGG - chr5_4:57667583-57667602 Msa0859320:intron 40.0%
!! GATTATTTCTGCAGGATGAC+GGG - chr5_4:57667663-57667682 Msa0859320:CDS 40.0%
AGGTCAAAAGTCTCAACACA+TGG + chr5_4:57667861-57667880 None:intergenic 40.0%
!! GAGAAGGTTTTTGTTGGTGA+AGG + chr5_4:57665849-57665868 None:intergenic 40.0%
CATCACAACAAAACCAATCG+CGG + chr5_4:57666135-57666154 None:intergenic 40.0%
!!! GCGATTGGTTTTGTTGTGAT+GGG - chr5_4:57666134-57666153 Msa0859320:three_prime_UTR 40.0%
! TTTGTTGTGATGGGATTCGT+TGG - chr5_4:57666143-57666162 Msa0859320:three_prime_UTR 40.0%
! TTGTTGTGATGGGATTCGTT+GGG - chr5_4:57666144-57666163 Msa0859320:three_prime_UTR 40.0%
CCAACGATGATGTTGTTGAT+TGG + chr5_4:57666194-57666213 None:intergenic 40.0%
CCAATCAACAACATCATCGT+TGG - chr5_4:57666191-57666210 Msa0859320:intron 40.0%
AACAACATCATCGTTGGATC+TGG - chr5_4:57666197-57666216 Msa0859320:intron 40.0%
!!! GCATAGTGGGTTTTAGTTGT+TGG - chr5_4:57666426-57666445 Msa0859320:intron 40.0%
!!! TAGTGGGTTTTAGTTGTTGG+AGG - chr5_4:57666429-57666448 Msa0859320:intron 40.0%
! TGCATGTTTGCTACAATGCA+TGG - chr5_4:57666483-57666502 Msa0859320:intron 40.0%
!! TCCGTTAGAAGTGTTAGTGT+CGG - chr5_4:57666659-57666678 Msa0859320:intron 40.0%
ACCGACACTAACACTTCTAA+CGG + chr5_4:57666663-57666682 None:intergenic 40.0%
!! GAATCAGGGGATTTTTGAAG+TGG - chr5_4:57666717-57666736 Msa0859320:intron 40.0%
! TTTGAAGTGGGTGATCTGTT+TGG - chr5_4:57666730-57666749 Msa0859320:intron 40.0%
!!! GATTTTCTCTGCATTGGTTG+TGG - chr5_4:57666899-57666918 Msa0859320:intron 40.0%
ACACAAGACACAACACTGAA+TGG + chr5_4:57667259-57667278 None:intergenic 40.0%
CTTCACAGAAATAGTTCCAC+GGG + chr5_4:57667378-57667397 None:intergenic 40.0%
GCTTCACAGAAATAGTTCCA+CGG + chr5_4:57667379-57667398 None:intergenic 40.0%
GCTGTAGCAACTTATGTCAA+AGG - chr5_4:57667426-57667445 Msa0859320:intron 40.0%
AATCACAGCAACCAACTCAA+TGG + chr5_4:57667550-57667569 None:intergenic 40.0%
AACTGCCTCATATGTCTTAC+AGG - chr5_4:57667583-57667602 Msa0859320:intron 40.0%
!! GATTATTTCTGCAGGATGAC+GGG - chr5_4:57667663-57667682 Msa0859320:CDS 40.0%
AGGTCAAAAGTCTCAACACA+TGG + chr5_4:57667861-57667880 None:intergenic 40.0%
GGTGAAGGAAGCAGATGAAT+TGG + chr5_4:57665834-57665853 None:intergenic 45.0%
TGGATCGAAGACGGAATCAA+TGG + chr5_4:57665934-57665953 None:intergenic 45.0%
TTCTCTGAGTGGATCGAAGA+CGG + chr5_4:57665943-57665962 None:intergenic 45.0%
TCTTTGGCGAATTCTCTGAG+TGG + chr5_4:57665954-57665973 None:intergenic 45.0%
AATTCGCCAAAGACAGCGTT+AGG - chr5_4:57665961-57665980 Msa0859320:three_prime_UTR 45.0%
AGGGGTTATACCTTTGCGAT+CGG + chr5_4:57666009-57666028 None:intergenic 45.0%
!!! CGCGATTGGTTTTGTTGTGA+TGG - chr5_4:57666133-57666152 Msa0859320:three_prime_UTR 45.0%
CATCATCGTTGGATCTGGTT+AGG - chr5_4:57666202-57666221 Msa0859320:intron 45.0%
CTTCTAACGGAAGATGTGTC+TGG + chr5_4:57666650-57666669 None:intergenic 45.0%
! GACTTTTGACCTGTTGCTCA+AGG - chr5_4:57667869-57667888 Msa0859320:CDS 45.0%
! TTTTGACCTGTTGCTCAAGG+AGG - chr5_4:57667872-57667891 Msa0859320:CDS 45.0%
! CTTTTTCCTCCTTGAGCAAC+AGG + chr5_4:57667881-57667900 None:intergenic 45.0%
TTGGCTTAATGACTCAGGTG+AGG - chr5_4:57668026-57668045 Msa0859320:five_prime_UTR 45.0%
TGGCTTAATGACTCAGGTGA+GGG - chr5_4:57668027-57668046 Msa0859320:five_prime_UTR 45.0%
GGTGAAGGAAGCAGATGAAT+TGG + chr5_4:57665834-57665853 None:intergenic 45.0%
TGGATCGAAGACGGAATCAA+TGG + chr5_4:57665934-57665953 None:intergenic 45.0%
TTCTCTGAGTGGATCGAAGA+CGG + chr5_4:57665943-57665962 None:intergenic 45.0%
TCTTTGGCGAATTCTCTGAG+TGG + chr5_4:57665954-57665973 None:intergenic 45.0%
AATTCGCCAAAGACAGCGTT+AGG - chr5_4:57665961-57665980 Msa0859320:three_prime_UTR 45.0%
AGGGGTTATACCTTTGCGAT+CGG + chr5_4:57666009-57666028 None:intergenic 45.0%
!!! CGCGATTGGTTTTGTTGTGA+TGG - chr5_4:57666133-57666152 Msa0859320:three_prime_UTR 45.0%
CATCATCGTTGGATCTGGTT+AGG - chr5_4:57666202-57666221 Msa0859320:intron 45.0%
CTTCTAACGGAAGATGTGTC+TGG + chr5_4:57666650-57666669 None:intergenic 45.0%
! GACTTTTGACCTGTTGCTCA+AGG - chr5_4:57667869-57667888 Msa0859320:CDS 45.0%
! TTTTGACCTGTTGCTCAAGG+AGG - chr5_4:57667872-57667891 Msa0859320:CDS 45.0%
! CTTTTTCCTCCTTGAGCAAC+AGG + chr5_4:57667881-57667900 None:intergenic 45.0%
TTGGCTTAATGACTCAGGTG+AGG - chr5_4:57668026-57668045 Msa0859320:five_prime_UTR 45.0%
TGGCTTAATGACTCAGGTGA+GGG - chr5_4:57668027-57668046 Msa0859320:five_prime_UTR 45.0%
AACGAGCCTAACGCTGTCTT+TGG + chr5_4:57665970-57665989 None:intergenic 50.0%
TACCTTTGCGATCGGGTTTG+TGG + chr5_4:57666001-57666020 None:intergenic 50.0%
GGGGTTATACCTTTGCGATC+GGG + chr5_4:57666008-57666027 None:intergenic 50.0%
ATAGTTCCACGGGCTCTTGA+GGG + chr5_4:57667368-57667387 None:intergenic 50.0%
AATAGTTCCACGGGCTCTTG+AGG + chr5_4:57667369-57667388 None:intergenic 50.0%
TGCTGTGATTGATTGCCGAG+CGG - chr5_4:57667560-57667579 Msa0859320:intron 50.0%
ACATATGAGGCAGTTCCGCT+CGG + chr5_4:57667578-57667597 None:intergenic 50.0%
ACGGGAATTACAATCGCCTC+AGG - chr5_4:57667681-57667700 Msa0859320:CDS 50.0%
CCTGCTTCACAACTTTCCTG+AGG + chr5_4:57667700-57667719 None:intergenic 50.0%
CCTCAGGAAAGTTGTGAAGC+AGG - chr5_4:57667697-57667716 Msa0859320:CDS 50.0%
CAGGAAAGTTGTGAAGCAGG+AGG - chr5_4:57667700-57667719 Msa0859320:CDS 50.0%
GAAAGTTGTGAAGCAGGAGG+AGG - chr5_4:57667703-57667722 Msa0859320:CDS 50.0%
AACGAGCCTAACGCTGTCTT+TGG + chr5_4:57665970-57665989 None:intergenic 50.0%
TACCTTTGCGATCGGGTTTG+TGG + chr5_4:57666001-57666020 None:intergenic 50.0%
GGGGTTATACCTTTGCGATC+GGG + chr5_4:57666008-57666027 None:intergenic 50.0%
ATAGTTCCACGGGCTCTTGA+GGG + chr5_4:57667368-57667387 None:intergenic 50.0%
AATAGTTCCACGGGCTCTTG+AGG + chr5_4:57667369-57667388 None:intergenic 50.0%
TGCTGTGATTGATTGCCGAG+CGG - chr5_4:57667560-57667579 Msa0859320:intron 50.0%
ACATATGAGGCAGTTCCGCT+CGG + chr5_4:57667578-57667597 None:intergenic 50.0%
ACGGGAATTACAATCGCCTC+AGG - chr5_4:57667681-57667700 Msa0859320:CDS 50.0%
CCTGCTTCACAACTTTCCTG+AGG + chr5_4:57667700-57667719 None:intergenic 50.0%
CCTCAGGAAAGTTGTGAAGC+AGG - chr5_4:57667697-57667716 Msa0859320:CDS 50.0%
CAGGAAAGTTGTGAAGCAGG+AGG - chr5_4:57667700-57667719 Msa0859320:CDS 50.0%
GAAAGTTGTGAAGCAGGAGG+AGG - chr5_4:57667703-57667722 Msa0859320:CDS 50.0%
TGCCACAAACCCGATCGCAA+AGG - chr5_4:57665996-57666015 Msa0859320:three_prime_UTR 55.0%
! GTTGCTGTTCGTACCGCGAT+TGG - chr5_4:57666119-57666138 Msa0859320:three_prime_UTR 55.0%
GACTCAGGTGAGGGAGTGAT+AGG - chr5_4:57668036-57668055 Msa0859320:five_prime_UTR 55.0%
TGCCACAAACCCGATCGCAA+AGG - chr5_4:57665996-57666015 Msa0859320:three_prime_UTR 55.0%
! GTTGCTGTTCGTACCGCGAT+TGG - chr5_4:57666119-57666138 Msa0859320:three_prime_UTR 55.0%
GACTCAGGTGAGGGAGTGAT+AGG - chr5_4:57668036-57668055 Msa0859320:five_prime_UTR 55.0%
CGCGGTACGAACAGCAACCT+TGG + chr5_4:57666117-57666136 None:intergenic 60.0%
CGCGGTACGAACAGCAACCT+TGG + chr5_4:57666117-57666136 None:intergenic 60.0%
! CCACGGGCTCTTGAGGGCAT+AGG + chr5_4:57667362-57667381 None:intergenic 65.0%
CCTATGCCCTCAAGAGCCCG+TGG - chr5_4:57667359-57667378 Msa0859320:intron 65.0%
! CCACGGGCTCTTGAGGGCAT+AGG + chr5_4:57667362-57667381 None:intergenic 65.0%
CCTATGCCCTCAAGAGCCCG+TGG - chr5_4:57667359-57667378 Msa0859320:intron 65.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 57665785 57668075 57665785 ID=Msa0859320;Name=Msa0859320
chr5_4 mRNA 57665785 57668075 57665785 ID=Msa0859320-mRNA-1;Parent=Msa0859320;Name=Msa0859320-mRNA-1;_AED=0.05;_eAED=0.05;_QI=139|1|1|1|0.5|1|3|399|69
chr5_4 exon 57665785 57666183 57665785 ID=Msa0859320-mRNA-1:exon:17547;Parent=Msa0859320-mRNA-1
chr5_4 exon 57667637 57667752 57667637 ID=Msa0859320-mRNA-1:exon:17546;Parent=Msa0859320-mRNA-1
chr5_4 exon 57667843 57668075 57667843 ID=Msa0859320-mRNA-1:exon:17545;Parent=Msa0859320-mRNA-1
chr5_4 five_prime_UTR 57667937 57668075 57667937 ID=Msa0859320-mRNA-1:five_prime_utr;Parent=Msa0859320-mRNA-1
chr5_4 CDS 57667843 57667936 57667843 ID=Msa0859320-mRNA-1:cds;Parent=Msa0859320-mRNA-1
chr5_4 CDS 57667637 57667752 57667637 ID=Msa0859320-mRNA-1:cds;Parent=Msa0859320-mRNA-1
chr5_4 three_prime_UTR 57665785 57666183 57665785 ID=Msa0859320-mRNA-1:three_prime_utr;Parent=Msa0859320-mRNA-1
Gene Sequence

>Msa0859320

ATGGACGCCATTGATTCCGTCTTCGATCCACTCAGAGAATTCGCCAAAGACAGCGTTAGGCTCGTTAAGCGTTGCCACAAACCCGATCGCAAAGAATTCTCCAAGGTTGCTGTTCGTACCGCGATTGGTTTTGTTGTGATGGGATTCGTTGGGTTTTTCGTTAAGCTTATCTTTATTCCAATCAACAACATCATCGTTGGATCTGGTTAG
Protein sequence

>Msa0859320

MDAIDSVFDPLREFAKDSVRLVKRCHKPDRKEFSKVAVRTAIGFVVMGFVGFFVKLIFIPINNIIVGSG*