Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0741690 | XP_003617415.1 | 95.349 | 86 | 4 | 0 | 1 | 86 | 1 | 86 | 5.76e-53 | 169 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0741690 | G7KD46 | 95.349 | 86 | 4 | 0 | 1 | 86 | 1 | 86 | 2.75e-53 | 169 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0081930 | Msa0741690 | 0.806585 | 7.658059e-50 | -8.615850e-47 |
| Msa0673460 | Msa0741690 | 0.804794 | 1.820332e-49 | -8.615850e-47 |
| Msa0741690 | Msa0743720 | 0.808347 | 3.239350e-50 | -8.615850e-47 |
| Msa0741690 | Msa0910000 | 0.801995 | 6.921396e-49 | -8.615850e-47 |
| Msa0741690 | Msa0947760 | 0.801116 | 1.048116e-48 | -8.615850e-47 |
| Msa0741690 | Msa1014540 | 0.810701 | 1.011081e-50 | -8.615850e-47 |
| Msa0741690 | Msa1082500 | 0.826144 | 3.186660e-54 | -8.615850e-47 |
| Msa0741690 | Msa1104090 | 0.804435 | 2.163558e-49 | -8.615850e-47 |
| Msa0741690 | Msa1129730 | 0.805182 | 1.510306e-49 | -8.615850e-47 |
| Msa0741690 | Msa1233890 | 0.809102 | 2.233409e-50 | -8.615850e-47 |
| Msa0741690 | Msa1249490 | 0.810113 | 1.354417e-50 | -8.615850e-47 |
| Msa0741690 | Msa1308620 | 0.807868 | 4.096477e-50 | -8.615850e-47 |
| Msa0562440 | Msa0741690 | 0.800373 | 1.486000e-48 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0741690 | MtrunA17_Chr5g0443071 | 95.349 | 86 | 4 | 0 | 1 | 86 | 1 | 86 | 5.30e-57 | 169 |
| Msa0741690 | MtrunA17_Chr4g0052071 | 50.000 | 74 | 30 | 3 | 17 | 86 | 3 | 73 | 2.95e-17 | 69.3 |
| Msa0741690 | MtrunA17_Chr4g0052051 | 50.667 | 75 | 29 | 3 | 17 | 86 | 3 | 74 | 2.10e-16 | 67.0 |
| Msa0741690 | MtrunA17_Chr3g0096231 | 56.250 | 64 | 26 | 2 | 21 | 83 | 3 | 65 | 6.49e-13 | 57.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0741690 | AT5G46295.1 | 60.465 | 43 | 16 | 1 | 44 | 86 | 30 | 71 | 3.36e-13 | 59.3 |
| Msa0741690 | AT1G06475.1 | 52.273 | 44 | 20 | 1 | 43 | 85 | 49 | 92 | 1.05e-11 | 55.8 |
Find 12 sgRNAs with CRISPR-Local
Find 13 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAAAGTGAAGAAAGAAATT+TGG | 0.267366 | 5_1:-61388860 | Msa0741690:CDS |
| TCATCTTCAAATGACATTCA+TGG | 0.338827 | 5_1:-61388799 | Msa0741690:CDS |
| CATGGAGATTCTCTGCGTTT+AGG | 0.350104 | 5_1:-61388836 | Msa0741690:CDS |
| TCATGGATACTATCGTGTTT+AGG | 0.371207 | 5_1:-61388782 | Msa0741690:CDS |
| TAGTTTGAGAAAGCGTTTGA+TGG | 0.413544 | 5_1:-61388910 | Msa0741690:CDS |
| GAAAGAAGAGTAGCATGTTA+TGG | 0.462290 | 5_1:-61388887 | Msa0741690:CDS |
| TGCTTCTTCTATCTTTGTTG+TGG | 0.470772 | 5_1:-61388717 | Msa0741690:CDS |
| AGTTTGAGAAAGCGTTTGAT+GGG | 0.536928 | 5_1:-61388909 | Msa0741690:CDS |
| GGAGATTCTCTGCGTTTAGG+TGG | 0.556332 | 5_1:-61388833 | Msa0741690:CDS |
| TGAAGAAAGAAATTTGGCCA+TGG | 0.584343 | 5_1:-61388854 | Msa0741690:CDS |
| AAGATAGAAGAAGCAAACAG+TGG | 0.638854 | 5_1:+61388725 | None:intergenic |
| CTAAACGCAGAGAATCTCCA+TGG | 0.659319 | 5_1:+61388837 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TCTTTCTAATCTCTTTTGTT+TGG | + | chr5_1:61388715-61388734 | None:intergenic | 25.0% |
| ! | AGAAAGTGAAGAAAGAAATT+TGG | - | chr5_1:61388786-61388805 | Msa0741690:CDS | 25.0% |
| TCATCTTCAAATGACATTCA+TGG | - | chr5_1:61388847-61388866 | Msa0741690:CDS | 30.0% | |
| !! | TAGTTTGAGAAAGCGTTTGA+TGG | - | chr5_1:61388736-61388755 | Msa0741690:CDS | 35.0% |
| !! | AGTTTGAGAAAGCGTTTGAT+GGG | - | chr5_1:61388737-61388756 | Msa0741690:CDS | 35.0% |
| GAAAGAAGAGTAGCATGTTA+TGG | - | chr5_1:61388759-61388778 | Msa0741690:CDS | 35.0% | |
| TGAAGAAAGAAATTTGGCCA+TGG | - | chr5_1:61388792-61388811 | Msa0741690:CDS | 35.0% | |
| TCATGGATACTATCGTGTTT+AGG | - | chr5_1:61388864-61388883 | Msa0741690:CDS | 35.0% | |
| AAGATAGAAGAAGCAAACAG+TGG | + | chr5_1:61388924-61388943 | None:intergenic | 35.0% | |
| TGCTTCTTCTATCTTTGTTG+TGG | - | chr5_1:61388929-61388948 | Msa0741690:CDS | 35.0% | |
| CTAAACGCAGAGAATCTCCA+TGG | + | chr5_1:61388812-61388831 | None:intergenic | 45.0% | |
| CATGGAGATTCTCTGCGTTT+AGG | - | chr5_1:61388810-61388829 | Msa0741690:CDS | 45.0% | |
| GGAGATTCTCTGCGTTTAGG+TGG | - | chr5_1:61388813-61388832 | Msa0741690:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_1 | gene | 61388704 | 61388964 | 61388704 | ID=Msa0741690;Name=Msa0741690 |
| chr5_1 | mRNA | 61388704 | 61388964 | 61388704 | ID=Msa0741690-mRNA-1;Parent=Msa0741690;Name=Msa0741690-mRNA-1;_AED=0.37;_eAED=0.37;_QI=0|-1|0|1|-1|1|1|0|86 |
| chr5_1 | exon | 61388704 | 61388964 | 61388704 | ID=Msa0741690-mRNA-1:exon:17824;Parent=Msa0741690-mRNA-1 |
| chr5_1 | CDS | 61388704 | 61388964 | 61388704 | ID=Msa0741690-mRNA-1:cds;Parent=Msa0741690-mRNA-1 |
| Gene Sequence |
| Protein sequence |
