AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0081930


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0081930 MtrunA17_Chr8g0345501 65.169 89 31 0 24 112 408 496 7.13e-34 123
Msa0081930 MtrunA17_Chr2g0313001 72.500 40 11 0 7 46 1 40 2.43e-13 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 33 sgRNAs with CRISPR-Local

Find 116 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAACTATCTCGAGCCAAATT+TGG 0.138676 1_2:+60682601 None:intergenic
TTACCTCTTTATCCCAAATT+TGG 0.184345 1_2:-60682614 None:intergenic
CCATCACCATCTGGAATTAT+TGG 0.275118 1_2:+60683743 None:intergenic
AAGAAATTGCTCACATTATA+TGG 0.286562 1_2:-60683206 Msa0081930:CDS
TGATTTCTTTGACTCATTCA+TGG 0.333269 1_2:-60683127 Msa0081930:intron
CCGTCATGGAAGCAATTATC+AGG 0.356730 1_2:-60683162 Msa0081930:CDS
AACTATCTCGAGCCAAATTT+GGG 0.363047 1_2:+60682602 None:intergenic
GTAGATTGAGTCAAAGTTGA+AGG 0.423072 1_2:-60682902 Msa0081930:exon
ACTTGTCATGATGCATGTTA+TGG 0.428964 1_2:+60682804 None:intergenic
AATGTGTACATTTCAAGTTA+TGG 0.444448 1_2:-60683825 Msa0081930:CDS
CAAACTCACCCATCACCATC+TGG 0.453372 1_2:+60683734 None:intergenic
CTCAGTTGATGCAAGAAGTT+CGG 0.458850 1_2:-60682929 Msa0081930:CDS
TACAAGGCTTAAGCAACAAT+TGG 0.471347 1_2:-60682872 Msa0081930:three_prime_UTR
TACATGGCAACCAGAGCATA+AGG 0.485592 1_2:-60682997 Msa0081930:CDS
CTAACATGGCTAGCTGAAGA+TGG 0.497267 1_2:+60683791 None:intergenic
TGCACATAGTAGTTCAGTTG+TGG 0.523431 1_2:-60683868 Msa0081930:five_prime_UTR
CCAATAATTCCAGATGGTGA+TGG 0.531511 1_2:-60683743 Msa0081930:CDS
GAGCCAAATTTGGGATAAAG+AGG 0.548288 1_2:+60682611 None:intergenic
CAATAATTCCAGATGGTGAT+GGG 0.552654 1_2:-60683742 Msa0081930:intron
CAACGGTGTCTCTACTAACA+TGG 0.557176 1_2:+60683777 None:intergenic
CCTGATAATTGCTTCCATGA+CGG 0.572248 1_2:+60683162 None:intergenic
CATAGTAGTTCAGTTGTGGT+TGG 0.585520 1_2:-60683864 Msa0081930:five_prime_UTR
TAGAAGAGGGAAAACCGTCA+TGG 0.601838 1_2:-60683176 Msa0081930:CDS
TATATGGTCTCAATTAGAAG+AGG 0.602911 1_2:-60683190 Msa0081930:CDS
TTTCTTTAACTACCAATGTG+TGG 0.607987 1_2:+60683223 None:intergenic
AAAATACCAATAATTCCAGA+TGG 0.610541 1_2:-60683749 Msa0081930:CDS
AGTTGAAGGAGGCAACTACA+AGG 0.610652 1_2:-60682888 Msa0081930:exon
ATATGGTCTCAATTAGAAGA+GGG 0.627134 1_2:-60683189 Msa0081930:CDS
CTTGAAATGTACACATTGCG+AGG 0.634227 1_2:+60683831 None:intergenic
CAGGTTTGATCACCACACAT+TGG 0.634316 1_2:-60683235 Msa0081930:CDS
GATTGAGTCAAAGTTGAAGG+AGG 0.637853 1_2:-60682899 Msa0081930:exon
ATGAGTCAAAGAAATCATCT+CGG 0.652827 1_2:+60683133 None:intergenic
CATGTTAGTAGAGACACCGT+TGG 0.707519 1_2:-60683776 Msa0081930:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CCAAATAATCATTATTATAT+GGG + chr1_2:60682843-60682862 None:intergenic 15.0%
!! TTATTTGGCTAATATAAATA+TGG - chr1_2:60682855-60682874 Msa0081930:three_prime_UTR 15.0%
!!! ATTTTGAATTAATATCTCTA+AGG - chr1_2:60683170-60683189 Msa0081930:CDS 15.0%
!! CCAAATAATCATTATTATAT+GGG + chr1_2:60682843-60682862 None:intergenic 15.0%
!! TTATTTGGCTAATATAAATA+TGG - chr1_2:60682855-60682874 Msa0081930:three_prime_UTR 15.0%
!!! ATTTTGAATTAATATCTCTA+AGG - chr1_2:60683170-60683189 Msa0081930:CDS 15.0%
!! CCCATATAATAATGATTATT+TGG - chr1_2:60682840-60682859 Msa0081930:three_prime_UTR 20.0%
!! GCCAAATAATCATTATTATA+TGG + chr1_2:60682844-60682863 None:intergenic 20.0%
!! TATTACAGAAAAAGTTTACA+TGG - chr1_2:60683533-60683552 Msa0081930:intron 20.0%
!! CCCATATAATAATGATTATT+TGG - chr1_2:60682840-60682859 Msa0081930:three_prime_UTR 20.0%
!! GCCAAATAATCATTATTATA+TGG + chr1_2:60682844-60682863 None:intergenic 20.0%
!! TATTACAGAAAAAGTTTACA+TGG - chr1_2:60683533-60683552 Msa0081930:intron 20.0%
!!! AATGTGTACATTTCAAGTTA+TGG - chr1_2:60682721-60682740 Msa0081930:three_prime_UTR 25.0%
!!! TATTGGTATTTTAGTACCAA+CGG + chr1_2:60682789-60682808 None:intergenic 25.0%
! AAAATACCAATAATTCCAGA+TGG - chr1_2:60682797-60682816 Msa0081930:three_prime_UTR 25.0%
!! TTGTGGTATTATGATTTTAG+TGG - chr1_2:60682938-60682957 Msa0081930:CDS 25.0%
!! TTTTCGTGTACATTATATTC+TGG - chr1_2:60682987-60683006 Msa0081930:CDS 25.0%
!!! AATTAATATCTCTAAGGCTT+TGG - chr1_2:60683176-60683195 Msa0081930:CDS 25.0%
!!! GTTTTTTCCTGAGAAAATAT+TGG - chr1_2:60683214-60683233 Msa0081930:CDS 25.0%
!!! TTGTTTGAAACACATTTTTC+AGG - chr1_2:60683292-60683311 Msa0081930:intron 25.0%
! AAGAAATTGCTCACATTATA+TGG - chr1_2:60683340-60683359 Msa0081930:intron 25.0%
!!! TTACTTAATCTGTTCTAAAG+AGG + chr1_2:60683483-60683502 None:intergenic 25.0%
!!! TGCGAAAAAACTTTGAAATA+AGG - chr1_2:60683578-60683597 Msa0081930:intron 25.0%
!!! GCGAAAAAACTTTGAAATAA+GGG - chr1_2:60683579-60683598 Msa0081930:intron 25.0%
!!! CGAAAAAACTTTGAAATAAG+GGG - chr1_2:60683580-60683599 Msa0081930:intron 25.0%
! AGCATATGCACAAAAAAAAA+TGG + chr1_2:60683899-60683918 None:intergenic 25.0%
!!! TTTTGTGCATATGCTATTTT+TGG - chr1_2:60683904-60683923 Msa0081930:five_prime_UTR 25.0%
!!! AATGTGTACATTTCAAGTTA+TGG - chr1_2:60682721-60682740 Msa0081930:three_prime_UTR 25.0%
!!! TATTGGTATTTTAGTACCAA+CGG + chr1_2:60682789-60682808 None:intergenic 25.0%
! AAAATACCAATAATTCCAGA+TGG - chr1_2:60682797-60682816 Msa0081930:three_prime_UTR 25.0%
!! TTGTGGTATTATGATTTTAG+TGG - chr1_2:60682938-60682957 Msa0081930:CDS 25.0%
!! TTTTCGTGTACATTATATTC+TGG - chr1_2:60682987-60683006 Msa0081930:CDS 25.0%
!!! AATTAATATCTCTAAGGCTT+TGG - chr1_2:60683176-60683195 Msa0081930:CDS 25.0%
!!! GTTTTTTCCTGAGAAAATAT+TGG - chr1_2:60683214-60683233 Msa0081930:CDS 25.0%
!!! TTGTTTGAAACACATTTTTC+AGG - chr1_2:60683292-60683311 Msa0081930:intron 25.0%
! AAGAAATTGCTCACATTATA+TGG - chr1_2:60683340-60683359 Msa0081930:intron 25.0%
!!! TTACTTAATCTGTTCTAAAG+AGG + chr1_2:60683483-60683502 None:intergenic 25.0%
!!! TGCGAAAAAACTTTGAAATA+AGG - chr1_2:60683578-60683597 Msa0081930:intron 25.0%
!!! GCGAAAAAACTTTGAAATAA+GGG - chr1_2:60683579-60683598 Msa0081930:intron 25.0%
!!! CGAAAAAACTTTGAAATAAG+GGG - chr1_2:60683580-60683599 Msa0081930:intron 25.0%
! AGCATATGCACAAAAAAAAA+TGG + chr1_2:60683899-60683918 None:intergenic 25.0%
!!! TTTTGTGCATATGCTATTTT+TGG - chr1_2:60683904-60683923 Msa0081930:five_prime_UTR 25.0%
! GATTCTGTTAACTTTCCTTT+TGG - chr1_2:60682887-60682906 Msa0081930:exon 30.0%
! TTTTCCTGAGAAAATATTGG+TGG - chr1_2:60683217-60683236 Msa0081930:CDS 30.0%
CAAGTAAAGTCTTAACTCAA+AGG + chr1_2:60683275-60683294 None:intergenic 30.0%
TTTCTTTAACTACCAATGTG+TGG + chr1_2:60683326-60683345 None:intergenic 30.0%
TATATGGTCTCAATTAGAAG+AGG - chr1_2:60683356-60683375 Msa0081930:intron 30.0%
ATATGGTCTCAATTAGAAGA+GGG - chr1_2:60683357-60683376 Msa0081930:intron 30.0%
ATGAGTCAAAGAAATCATCT+CGG + chr1_2:60683416-60683435 None:intergenic 30.0%
TGATTTCTTTGACTCATTCA+TGG - chr1_2:60683419-60683438 Msa0081930:intron 30.0%
! GATTCTGTTAACTTTCCTTT+TGG - chr1_2:60682887-60682906 Msa0081930:exon 30.0%
! TTTTCCTGAGAAAATATTGG+TGG - chr1_2:60683217-60683236 Msa0081930:CDS 30.0%
CAAGTAAAGTCTTAACTCAA+AGG + chr1_2:60683275-60683294 None:intergenic 30.0%
TTTCTTTAACTACCAATGTG+TGG + chr1_2:60683326-60683345 None:intergenic 30.0%
TATATGGTCTCAATTAGAAG+AGG - chr1_2:60683356-60683375 Msa0081930:intron 30.0%
ATATGGTCTCAATTAGAAGA+GGG - chr1_2:60683357-60683376 Msa0081930:intron 30.0%
ATGAGTCAAAGAAATCATCT+CGG + chr1_2:60683416-60683435 None:intergenic 30.0%
TGATTTCTTTGACTCATTCA+TGG - chr1_2:60683419-60683438 Msa0081930:intron 30.0%
CAATAATTCCAGATGGTGAT+GGG - chr1_2:60682804-60682823 Msa0081930:three_prime_UTR 35.0%
! GCATTGTTACTTGATCCAAA+AGG + chr1_2:60682905-60682924 None:intergenic 35.0%
GTTGCTGAACATGCTATATA+TGG - chr1_2:60683073-60683092 Msa0081930:intron 35.0%
GTAGATTGAGTCAAAGTTGA+AGG - chr1_2:60683644-60683663 Msa0081930:intron 35.0%
TACAAGGCTTAAGCAACAAT+TGG - chr1_2:60683674-60683693 Msa0081930:intron 35.0%
ACTTGTCATGATGCATGTTA+TGG + chr1_2:60683745-60683764 None:intergenic 35.0%
CAATAATTCCAGATGGTGAT+GGG - chr1_2:60682804-60682823 Msa0081930:three_prime_UTR 35.0%
! GCATTGTTACTTGATCCAAA+AGG + chr1_2:60682905-60682924 None:intergenic 35.0%
GTTGCTGAACATGCTATATA+TGG - chr1_2:60683073-60683092 Msa0081930:intron 35.0%
GTAGATTGAGTCAAAGTTGA+AGG - chr1_2:60683644-60683663 Msa0081930:intron 35.0%
TACAAGGCTTAAGCAACAAT+TGG - chr1_2:60683674-60683693 Msa0081930:intron 35.0%
ACTTGTCATGATGCATGTTA+TGG + chr1_2:60683745-60683764 None:intergenic 35.0%
TGCACATAGTAGTTCAGTTG+TGG - chr1_2:60682678-60682697 Msa0081930:three_prime_UTR 40.0%
CATAGTAGTTCAGTTGTGGT+TGG - chr1_2:60682682-60682701 Msa0081930:three_prime_UTR 40.0%
CTTGAAATGTACACATTGCG+AGG + chr1_2:60682718-60682737 None:intergenic 40.0%
! CCATCACCATCTGGAATTAT+TGG + chr1_2:60682806-60682825 None:intergenic 40.0%
CCAATAATTCCAGATGGTGA+TGG - chr1_2:60682803-60682822 Msa0081930:three_prime_UTR 40.0%
!! ATCTCTAAGGCTTTGGAACA+TGG - chr1_2:60683183-60683202 Msa0081930:CDS 40.0%
! TGCACCACCAATATTTTCTC+AGG + chr1_2:60683224-60683243 None:intergenic 40.0%
GTGCAAGTAGTTAGTTGTGT+TGG - chr1_2:60683239-60683258 Msa0081930:intron 40.0%
CCTGATAATTGCTTCCATGA+CGG + chr1_2:60683387-60683406 None:intergenic 40.0%
! CTCAGTTGATGCAAGAAGTT+CGG - chr1_2:60683617-60683636 Msa0081930:intron 40.0%
GATTGAGTCAAAGTTGAAGG+AGG - chr1_2:60683647-60683666 Msa0081930:intron 40.0%
TGCACATAGTAGTTCAGTTG+TGG - chr1_2:60682678-60682697 Msa0081930:three_prime_UTR 40.0%
CATAGTAGTTCAGTTGTGGT+TGG - chr1_2:60682682-60682701 Msa0081930:three_prime_UTR 40.0%
CTTGAAATGTACACATTGCG+AGG + chr1_2:60682718-60682737 None:intergenic 40.0%
! CCATCACCATCTGGAATTAT+TGG + chr1_2:60682806-60682825 None:intergenic 40.0%
CCAATAATTCCAGATGGTGA+TGG - chr1_2:60682803-60682822 Msa0081930:three_prime_UTR 40.0%
!! ATCTCTAAGGCTTTGGAACA+TGG - chr1_2:60683183-60683202 Msa0081930:CDS 40.0%
! TGCACCACCAATATTTTCTC+AGG + chr1_2:60683224-60683243 None:intergenic 40.0%
GTGCAAGTAGTTAGTTGTGT+TGG - chr1_2:60683239-60683258 Msa0081930:intron 40.0%
CCTGATAATTGCTTCCATGA+CGG + chr1_2:60683387-60683406 None:intergenic 40.0%
! CTCAGTTGATGCAAGAAGTT+CGG - chr1_2:60683617-60683636 Msa0081930:intron 40.0%
GATTGAGTCAAAGTTGAAGG+AGG - chr1_2:60683647-60683666 Msa0081930:intron 40.0%
! CTAACATGGCTAGCTGAAGA+TGG + chr1_2:60682758-60682777 None:intergenic 45.0%
CAACGGTGTCTCTACTAACA+TGG + chr1_2:60682772-60682791 None:intergenic 45.0%
CATGTTAGTAGAGACACCGT+TGG - chr1_2:60682770-60682789 Msa0081930:three_prime_UTR 45.0%
ATGCAAGACTGTTGTGCTTG+TGG - chr1_2:60682921-60682940 Msa0081930:CDS 45.0%
!! CTAAGGCTTTGGAACATGGT+TGG - chr1_2:60683187-60683206 Msa0081930:CDS 45.0%
CAGGTTTGATCACCACACAT+TGG - chr1_2:60683311-60683330 Msa0081930:intron 45.0%
TAGAAGAGGGAAAACCGTCA+TGG - chr1_2:60683370-60683389 Msa0081930:intron 45.0%
CCGTCATGGAAGCAATTATC+AGG - chr1_2:60683384-60683403 Msa0081930:intron 45.0%
TACATGGCAACCAGAGCATA+AGG - chr1_2:60683549-60683568 Msa0081930:intron 45.0%
! CGCACTTTTACCTTATGCTC+TGG + chr1_2:60683562-60683581 None:intergenic 45.0%
AGTTGAAGGAGGCAACTACA+AGG - chr1_2:60683658-60683677 Msa0081930:intron 45.0%
! CTAACATGGCTAGCTGAAGA+TGG + chr1_2:60682758-60682777 None:intergenic 45.0%
CAACGGTGTCTCTACTAACA+TGG + chr1_2:60682772-60682791 None:intergenic 45.0%
CATGTTAGTAGAGACACCGT+TGG - chr1_2:60682770-60682789 Msa0081930:three_prime_UTR 45.0%
ATGCAAGACTGTTGTGCTTG+TGG - chr1_2:60682921-60682940 Msa0081930:CDS 45.0%
!! CTAAGGCTTTGGAACATGGT+TGG - chr1_2:60683187-60683206 Msa0081930:CDS 45.0%
CAGGTTTGATCACCACACAT+TGG - chr1_2:60683311-60683330 Msa0081930:intron 45.0%
TAGAAGAGGGAAAACCGTCA+TGG - chr1_2:60683370-60683389 Msa0081930:intron 45.0%
CCGTCATGGAAGCAATTATC+AGG - chr1_2:60683384-60683403 Msa0081930:intron 45.0%
TACATGGCAACCAGAGCATA+AGG - chr1_2:60683549-60683568 Msa0081930:intron 45.0%
! CGCACTTTTACCTTATGCTC+TGG + chr1_2:60683562-60683581 None:intergenic 45.0%
AGTTGAAGGAGGCAACTACA+AGG - chr1_2:60683658-60683677 Msa0081930:intron 45.0%
CAAACTCACCCATCACCATC+TGG + chr1_2:60682815-60682834 None:intergenic 50.0%
CAAACTCACCCATCACCATC+TGG + chr1_2:60682815-60682834 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 60682616 60683952 60682616 ID=Msa0081930;Name=Msa0081930
chr1_2 mRNA 60682616 60683952 60682616 ID=Msa0081930-mRNA-1;Parent=Msa0081930;Name=Msa0081930-mRNA-1;_AED=0.28;_eAED=0.28;_QI=106|1|1|1|1|1|3|289|117
chr1_2 exon 60682616 60683027 60682616 ID=Msa0081930-mRNA-1:exon:19826;Parent=Msa0081930-mRNA-1
chr1_2 exon 60683128 60683254 60683128 ID=Msa0081930-mRNA-1:exon:19825;Parent=Msa0081930-mRNA-1
chr1_2 exon 60683743 60683952 60683743 ID=Msa0081930-mRNA-1:exon:19824;Parent=Msa0081930-mRNA-1
chr1_2 five_prime_UTR 60683847 60683952 60683847 ID=Msa0081930-mRNA-1:five_prime_utr;Parent=Msa0081930-mRNA-1
chr1_2 CDS 60683743 60683846 60683743 ID=Msa0081930-mRNA-1:cds;Parent=Msa0081930-mRNA-1
chr1_2 CDS 60683128 60683254 60683128 ID=Msa0081930-mRNA-1:cds;Parent=Msa0081930-mRNA-1
chr1_2 CDS 60682905 60683027 60682905 ID=Msa0081930-mRNA-1:cds;Parent=Msa0081930-mRNA-1
chr1_2 three_prime_UTR 60682616 60682904 60682616 ID=Msa0081930-mRNA-1:three_prime_utr;Parent=Msa0081930-mRNA-1
Gene Sequence

>Msa0081930

ATGTGTACATTTCAAGTTATGGTCAATAGAGCTCCATCTTCAGCTAGCCATGTTAGTAGAGACACCGTTGGTACTAAAATACCAATAATTCCAGATGGTGATGGGTTTGATCACCACACATTGGTAGTTAAAGAAATTGCTCACATTATATGGTCTCAATTAGAAGAGGGAAAACCGTCATGGAAGCAATTATCAGGAAATCACCGAGATGATTTCTTTGACTCATTCATGAAAAAGTTTACATGGCAACCAGAGCATAAGGTAAAAGTGCGAAAAAACTTTGAAATAAGGGGTGCAGCTAAGATGTCTCAGTTGATGCAAGAAGTTCGGAAAAGTAGATTGAGTCAAAGTTGA

Protein sequence

>Msa0081930

MCTFQVMVNRAPSSASHVSRDTVGTKIPIIPDGDGFDHHTLVVKEIAHIIWSQLEEGKPSWKQLSGNHRDDFFDSFMKKFTWQPEHKVKVRKNFEIRGAAKMSQLMQEVRKSRLSQS*