Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0748940 | G7KI13 | 94.382 | 89 | 5 | 0 | 1 | 89 | 1 | 89 | 1.08e-50 | 163 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0718120 | Msa0748940 | -0.815581 | 8.589066e-52 | -8.615850e-47 |
| Msa0748940 | Msa0838990 | -0.810303 | 1.232561e-50 | -8.615850e-47 |
| Msa0748940 | Msa1207000 | 0.826215 | 3.065430e-54 | -8.615850e-47 |
| Msa0748940 | Msa1207090 | 0.825493 | 4.547940e-54 | -8.615850e-47 |
| Msa0748940 | Msa1257170 | 0.825118 | 5.579605e-54 | -8.615850e-47 |
| Msa0748940 | Msa1258600 | 0.826898 | 2.107206e-54 | -8.615850e-47 |
| Msa0748940 | Msa1302120 | 0.823682 | 1.214276e-53 | -8.615850e-47 |
| Msa0748940 | Msa1400940 | 0.826538 | 2.568580e-54 | -8.615850e-47 |
| Msa0482360 | Msa0748940 | 0.853128 | 2.931424e-61 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0748940 | MtrunA17_Chr6g0487201 | 94.382 | 89 | 5 | 0 | 1 | 89 | 1 | 89 | 2.07e-54 | 163 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 28 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGGAGCTGCTTTGGATTCTA+AGG | 0.294697 | 5_2:+2311887 | Msa0748940:CDS |
| TCTTCATGATCTTCGAAATT+CGG | 0.342704 | 5_2:-2311912 | None:intergenic |
| TGCAGAACATGGAGCTGCTT+TGG | 0.354322 | 5_2:+2311878 | Msa0748940:CDS |
| CCTCCGTGACCTCCTTTCTT+CGG | 0.436804 | 5_2:-2311816 | None:intergenic |
| GCGAGATTCCGATGCGGAGG+AGG | 0.453286 | 5_2:-2311757 | None:intergenic |
| GGCGGTGACGGTGTCGGCAT+CGG | 0.469546 | 5_2:-2311946 | None:intergenic |
| CTTCAGATCGGTGCAGAACA+TGG | 0.498685 | 5_2:+2311867 | Msa0748940:CDS |
| ATGAGTGGTTCGCCGAAGAA+AGG | 0.506182 | 5_2:+2311804 | Msa0748940:CDS |
| CGGAAAATCCTCCTCCGCAT+CGG | 0.554876 | 5_2:+2311749 | Msa0748940:CDS |
| AACAGTAACAGCGGCGGTGA+CGG | 0.557718 | 5_2:-2311958 | None:intergenic |
| AAAGATCGCAAATCATCCTC+TGG | 0.561958 | 5_2:+2311780 | Msa0748940:CDS |
| GTCACGGAGGGAAGTTCACA+TGG | 0.573877 | 5_2:+2311829 | Msa0748940:CDS |
| AAAAGCCACAAATCCACCAC+CGG | 0.574764 | 5_2:+2311729 | Msa0748940:CDS |
| GGAGGGAAGTTCACATGGAT+CGG | 0.576604 | 5_2:+2311834 | Msa0748940:CDS |
| TGTTCTGCACCGATCTGAAG+AGG | 0.576691 | 5_2:-2311864 | None:intergenic |
| AACAGCGGCGGTGACGGTGT+CGG | 0.580776 | 5_2:-2311952 | None:intergenic |
| GGATCTGATCCTCTTCAGAT+CGG | 0.604184 | 5_2:+2311855 | Msa0748940:CDS |
| AAATCATCCTCTGGAATGAG+TGG | 0.616881 | 5_2:+2311789 | Msa0748940:CDS |
| CGAAGAAAGGAGGTCACGGA+GGG | 0.619660 | 5_2:+2311817 | Msa0748940:CDS |
| ACTTCAACAATGAAATCCAC+CGG | 0.621434 | 5_2:+2311705 | None:intergenic |
| AGTGGTTCGCCGAAGAAAGG+AGG | 0.624956 | 5_2:+2311807 | Msa0748940:CDS |
| TCGCCGAAGAAAGGAGGTCA+CGG | 0.629596 | 5_2:+2311813 | Msa0748940:CDS |
| TTTGCGAGATTCCGATGCGG+AGG | 0.641066 | 5_2:-2311760 | None:intergenic |
| CGGCGAACCACTCATTCCAG+AGG | 0.662672 | 5_2:-2311796 | None:intergenic |
| CCGAAGAAAGGAGGTCACGG+AGG | 0.683228 | 5_2:+2311816 | Msa0748940:CDS |
| CCGCAATCAAACAGTAACAG+CGG | 0.719151 | 5_2:-2311967 | None:intergenic |
| ATCTTTGCGAGATTCCGATG+CGG | 0.720281 | 5_2:-2311763 | None:intergenic |
| CAATCAAACAGTAACAGCGG+CGG | 0.733723 | 5_2:-2311964 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TCTTCATGATCTTCGAAATT+CGG | - | chr5_2:2311915-2311934 | None:intergenic | 30.0% | |
| AAAGATCGCAAATCATCCTC+TGG | + | chr5_2:2311780-2311799 | Msa0748940:CDS | 40.0% | |
| !! | AAATCATCCTCTGGAATGAG+TGG | + | chr5_2:2311789-2311808 | Msa0748940:CDS | 40.0% |
| !! | GGTGGATTTGTGGCTTTTTC+CGG | - | chr5_2:2311727-2311746 | None:intergenic | 45.0% |
| AAAAGCCACAAATCCACCAC+CGG | + | chr5_2:2311729-2311748 | Msa0748940:CDS | 45.0% | |
| !!! | ATTTTCCGGTGGTGGATTTG+TGG | - | chr5_2:2311737-2311756 | None:intergenic | 45.0% |
| ATCTTTGCGAGATTCCGATG+CGG | - | chr5_2:2311766-2311785 | None:intergenic | 45.0% | |
| GGATCTGATCCTCTTCAGAT+CGG | + | chr5_2:2311855-2311874 | Msa0748940:CDS | 45.0% | |
| ! | TGGAGCTGCTTTGGATTCTA+AGG | + | chr5_2:2311887-2311906 | Msa0748940:CDS | 45.0% |
| !! | GGATTTGTGGCTTTTTCCGG+TGG | - | chr5_2:2311724-2311743 | None:intergenic | 50.0% |
| ATGAGTGGTTCGCCGAAGAA+AGG | + | chr5_2:2311804-2311823 | Msa0748940:CDS | 50.0% | |
| GGAGGGAAGTTCACATGGAT+CGG | + | chr5_2:2311834-2311853 | Msa0748940:CDS | 50.0% | |
| ! | TGTTCTGCACCGATCTGAAG+AGG | - | chr5_2:2311867-2311886 | None:intergenic | 50.0% |
| CTTCAGATCGGTGCAGAACA+TGG | + | chr5_2:2311867-2311886 | Msa0748940:CDS | 50.0% | |
| TGCAGAACATGGAGCTGCTT+TGG | + | chr5_2:2311878-2311897 | Msa0748940:CDS | 50.0% | |
| ! | CGATGCGGAGGAGGATTTTC+CGG | - | chr5_2:2311751-2311770 | None:intergenic | 55.0% |
| CGGAAAATCCTCCTCCGCAT+CGG | + | chr5_2:2311749-2311768 | Msa0748940:CDS | 55.0% | |
| TTTGCGAGATTCCGATGCGG+AGG | - | chr5_2:2311763-2311782 | None:intergenic | 55.0% | |
| AGTGGTTCGCCGAAGAAAGG+AGG | + | chr5_2:2311807-2311826 | Msa0748940:CDS | 55.0% | |
| TCGCCGAAGAAAGGAGGTCA+CGG | + | chr5_2:2311813-2311832 | Msa0748940:CDS | 55.0% | |
| CCTCCGTGACCTCCTTTCTT+CGG | - | chr5_2:2311819-2311838 | None:intergenic | 55.0% | |
| CGAAGAAAGGAGGTCACGGA+GGG | + | chr5_2:2311817-2311836 | Msa0748940:CDS | 55.0% | |
| GTCACGGAGGGAAGTTCACA+TGG | + | chr5_2:2311829-2311848 | Msa0748940:CDS | 55.0% | |
| AACAGTAACAGCGGCGGTGA+CGG | - | chr5_2:2311961-2311980 | None:intergenic | 55.0% | |
| ! | GGAGGAGGATTTTCCGGTGG+TGG | - | chr5_2:2311745-2311764 | None:intergenic | 60.0% |
| ! | TGCGGAGGAGGATTTTCCGG+TGG | - | chr5_2:2311748-2311767 | None:intergenic | 60.0% |
| CGGCGAACCACTCATTCCAG+AGG | - | chr5_2:2311799-2311818 | None:intergenic | 60.0% | |
| CCGAAGAAAGGAGGTCACGG+AGG | + | chr5_2:2311816-2311835 | Msa0748940:CDS | 60.0% | |
| GCGAGATTCCGATGCGGAGG+AGG | - | chr5_2:2311760-2311779 | None:intergenic | 65.0% | |
| ! | AACAGCGGCGGTGACGGTGT+CGG | - | chr5_2:2311955-2311974 | None:intergenic | 65.0% |
| !! | GGCGGTGACGGTGTCGGCAT+CGG | - | chr5_2:2311949-2311968 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_2 | gene | 2311714 | 2311983 | 2311714 | ID=Msa0748940;Name=Msa0748940 |
| chr5_2 | mRNA | 2311714 | 2311983 | 2311714 | ID=Msa0748940-mRNA-1;Parent=Msa0748940;Name=Msa0748940-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|-1|0|1|-1|1|1|0|89 |
| chr5_2 | exon | 2311714 | 2311983 | 2311714 | ID=Msa0748940-mRNA-1:exon:656;Parent=Msa0748940-mRNA-1 |
| chr5_2 | CDS | 2311714 | 2311983 | 2311714 | ID=Msa0748940-mRNA-1:cds;Parent=Msa0748940-mRNA-1 |
| Gene Sequence |
| Protein sequence |
