AlfalfaGEDB | Msa1343250

M. sativa cultivar zhongmu-4 / Msa1343250

KEGG: -
GO: -
PFAM: PF00010.29

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1343250	XP_003593344.3	95.751	353	15	0	1	353	1	353	0.0	703

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1343250	sp\|Q9T072\|BH025_ARATH	56.757	185	72	4	173	353	148	328	9.82e-59	196

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1343250	A0A396J145	95.751	353	15	0	1	353	1	353	0.0	703

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TFs/TRs:

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Gene ID	Type	Classification
Msa1343250	TF	bHLH

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0003630	Msa1343250	0.814009	1.915761e-51	-8.615850e-47
Msa0007990	Msa1343250	0.823816	1.130023e-53	-8.615850e-47
Msa0009790	Msa1343250	0.802247	6.140649e-49	-8.615850e-47
Msa0017320	Msa1343250	0.802263	6.095589e-49	-8.615850e-47
Msa0042550	Msa1343250	0.879633	1.062241e-69	-8.615850e-47
Msa0055070	Msa1343250	0.806863	6.688707e-50	-8.615850e-47
Msa0058310	Msa1343250	0.823292	1.498493e-53	-8.615850e-47
Msa0092410	Msa1343250	0.849205	3.755509e-60	-8.615850e-47
Msa0101020	Msa1343250	0.807333	5.321515e-50	-8.615850e-47
Msa0102140	Msa1343250	0.804457	2.140764e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1343250	MtrunA17_Chr2g0280161	95.751	353	15	0	1	353	1	353	0.0	703
Msa1343250	MtrunA17_Chr4g0049331	55.587	349	111	10	7	353	4	310	2.08e-115	337
Msa1343250	MtrunA17_Chr4g0054561	39.368	348	175	13	5	345	2	320	3.45e-59	194
Msa1343250	MtrunA17_Chr5g0400661	38.192	343	166	7	20	346	18	330	1.19e-58	192
Msa1343250	MtrunA17_Chr4g0054551	39.368	348	175	13	5	345	2	320	2.72e-58	191
Msa1343250	MtrunA17_Chr5g0400621	52.632	190	87	2	166	353	144	332	5.39e-55	183
Msa1343250	MtrunA17_Chr5g0400671	34.393	346	199	10	5	348	2	321	1.37e-50	171
Msa1343250	MtrunA17_Chr4g0054581	44.643	224	107	5	138	353	163	377	2.96e-49	169
Msa1343250	MtrunA17_Chr8g0336681	42.081	221	116	4	134	346	115	331	1.80e-48	166
Msa1343250	MtrunA17_Chr6g0468491	46.667	195	99	4	161	353	121	312	2.24e-48	165

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1343250	AT4G37850.2	56.757	185	72	4	173	353	148	328	9.98e-60	196
Msa1343250	AT4G37850.1	56.757	185	72	4	173	353	148	328	9.98e-60	196
Msa1343250	AT2G22760.1	49.153	177	87	3	175	348	117	293	1.02e-48	166
Msa1343250	AT2G22770.1	46.809	188	86	2	177	350	132	319	6.69e-45	157
Msa1343250	AT2G22750.2	50.811	185	84	3	173	352	122	304	3.44e-43	152
Msa1343250	AT2G22750.3	50.811	185	84	3	173	352	150	332	6.53e-43	152
Msa1343250	AT2G22750.5	50.811	185	84	3	173	352	146	328	6.81e-43	152
Msa1343250	AT2G22750.1	50.811	185	83	4	173	352	122	303	6.84e-42	148
Msa1343250	AT2G22760.2	47.468	158	81	2	175	330	117	274	1.21e-41	147
Msa1343250	AT2G22750.4	50.811	185	83	4	173	352	150	331	1.66e-41	148

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Find 66 sgRNAs with CRISPR-Local

Find 126 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACAGTTAAGAAATAACAGC+TGG	0.500916	tig0006328:-50616	Msa1343250:CDS
AACTCTGCAGGGGATAATGG+AGG	0.663168	tig0006328:-50810	Msa1343250:intron
AACTTAACTGCTTCTGCTTT+TGG	0.208058	tig0006328:-50734	Msa1343250:CDS
AAGTAATAATCATGTTTGCA+TGG	0.557909	tig0006328:-48827	Msa1343250:three_prime_UTR
AATAAATATCTTACCATTTC+AGG	0.175967	tig0006328:+51042	None:intergenic
AATAATCATGTTTGCATGGC+GGG	0.428743	tig0006328:-48823	Msa1343250:three_prime_UTR
AATAGCAGTTCCTTGACTTT+TGG	0.241912	tig0006328:-49403	Msa1343250:CDS
ACCGGTCTTTCGTTTCCGGT+AGG	0.581772	tig0006328:+50641	None:intergenic
AGAAGAGTTGTATTATTATA+TGG	0.309218	tig0006328:+51320	None:intergenic
AGAAGCCTATATTTGAAACA+AGG	0.653550	tig0006328:+51382	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TAGTGAACAAAACAAAACTC+AGG	-	tig0006328:48826-48845	Msa1343250:three_prime_UTR	30.0%
TATTGGTATCAATAGAGCTT+AGG	+	tig0006328:49341-49360	None:intergenic	30.0%
GTTGTTGAAGTTTGGATTAT+TGG	+	tig0006328:49412-49431	None:intergenic	30.0%
AACAGTTAAGAAATAACAGC+TGG	-	tig0006328:49478-49497	Msa1343250:CDS	30.0%
TCATATTGTGTATCTGATGT+TGG	+	tig0006328:49522-49541	None:intergenic	30.0%
TGTGTAATTCAAATCAGCAA+AGG	+	tig0006328:49571-49590	None:intergenic	30.0%
CATTATTCGATACCTTCTTT+AGG	+	tig0006328:49854-49873	None:intergenic	30.0%
AACATCGTGATATCTTTGTT+AGG	-	tig0006328:49945-49964	Msa1343250:intron	30.0%
TCGATATACCAACTATAACT+CGG	-	tig0006328:50129-50148	Msa1343250:intron	30.0%
ACAAAAAAGGAAGAAAACTG+TGG	-	tig0006328:50456-50475	Msa1343250:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0006328	gene	48705	51411	48705	ID=Msa1343250;Name=Msa1343250
tig0006328	mRNA	48705	51411	48705	ID=Msa1343250-mRNA-1;Parent=Msa1343250;Name=Msa1343250-mRNA-1;_AED=0.02;_eAED=0.02;_QI=179\|1\|0.8\|1\|0.75\|0.6\|5\|207\|353
tig0006328	exon	48705	48986	48705	ID=Msa1343250-mRNA-1:exon:4035;Parent=Msa1343250-mRNA-1
tig0006328	exon	49369	49740	49369	ID=Msa1343250-mRNA-1:exon:4034;Parent=Msa1343250-mRNA-1
tig0006328	exon	50256	50822	50256	ID=Msa1343250-mRNA-1:exon:4033;Parent=Msa1343250-mRNA-1
tig0006328	exon	51056	51104	51056	ID=Msa1343250-mRNA-1:exon:4032;Parent=Msa1343250-mRNA-1
tig0006328	exon	51234	51411	51234	ID=Msa1343250-mRNA-1:exon:4031;Parent=Msa1343250-mRNA-1
tig0006328	five_prime_UTR	51234	51411	51234	ID=Msa1343250-mRNA-1:five_prime_utr;Parent=Msa1343250-mRNA-1
tig0006328	five_prime_UTR	51104	51104	51104	ID=Msa1343250-mRNA-1:five_prime_utr;Parent=Msa1343250-mRNA-1
tig0006328	CDS	51056	51103	51056	ID=Msa1343250-mRNA-1:cds;Parent=Msa1343250-mRNA-1
tig0006328	CDS	50256	50822	50256	ID=Msa1343250-mRNA-1:cds;Parent=Msa1343250-mRNA-1
tig0006328	CDS	49369	49740	49369	ID=Msa1343250-mRNA-1:cds;Parent=Msa1343250-mRNA-1
tig0006328	CDS	48912	48986	48912	ID=Msa1343250-mRNA-1:cds;Parent=Msa1343250-mRNA-1
tig0006328	three_prime_UTR	48705	48911	48705	ID=Msa1343250-mRNA-1:three_prime_utr;Parent=Msa1343250-mRNA-1

Gene Sequence

>Msa1343250

ATGAGTTCTCATATGGAGATTTCATCTATCAGAGGGTTACCTGAAATGGGGATAATGGAGGATCCTAATTTTTTACACCACTTCAATAATCACCTAAGCTCTATTGATACCAATAACTTAACTGCTTCTGCTTTTGGAGATGCTTTACAAAAACATATTTTGTCCAATAATCCAAACTTCAACAACAAAACATGCATGGAGACTTCGCCTACCGGAAACGAAAGACCGGTGAAACAGTTAAGAAATAACAGCTGGAATTACAACAATAGTCCTCCAACATCAGATACACAATATGATAATTGTTGCTCTAATAATCTTCTTTCCTTTGCTGATTTGAATTACACAAATCAGTTGGGACTATTGAAGCCTAAGTCTGAGATGGTGTGTCCGAAAATCGATAACACTAGTACTCTTGCAAACATGTTGATCGCTCAAGGTAACTTGTTTGGGAATCAAAACCATGTCTTTAAAGCTGTTCAAGAGGCTAAAGATATTGAGAATCGTCCTAATAAAATCTCTCAAGCTCATGATCACATCGTAGCGGAGAGGAAGCGTCGCGAGAAGCTTAGCCAACGATTCATCGCTCTGTCTGCTCTTGTTCCAAACCTAAAGAAGATGGATAAAGCTTCTGTTCTTGGAGAAGCTATAAGGTACTTAAAGCAAATGGAAGAGAAAGTGAGTGTTCTTGAGGAGGAACAAAAAAGGAAGAAAACTGTGGAATCTGTTGTAATAGTGAAGAAATCTCAACTTTCTATTAATGATGCTGAAAACTCTTGTTCAGACACTGATAGTACATTTGATGAGACATTACCTGAAATTGAAGCAAGATTTTGTGAAAGAAGTGTCCTCATAAGACTACATTGTTTGAAGAGTCAAGGAGTTATTGAGAAAATTATGAGTGAAATTGAGAAACTTCATCTGAAAGTCATCAATAGCAGTTCCTTGACTTTTGGGAATTTCACCCTTGATATAACCATCATTGCTCAGATGGATGTGGAATTTTGCATGACAGTGAAGGATCTTGTGAGGAAGCTACGTTCAGCATATTCATCTTTCATGTGA

Protein sequence

>Msa1343250

MSSHMEISSIRGLPEMGIMEDPNFLHHFNNHLSSIDTNNLTASAFGDALQKHILSNNPNFNNKTCMETSPTGNERPVKQLRNNSWNYNNSPPTSDTQYDNCCSNNLLSFADLNYTNQLGLLKPKSEMVCPKIDNTSTLANMLIAQGNLFGNQNHVFKAVQEAKDIENRPNKISQAHDHIVAERKRREKLSQRFIALSALVPNLKKMDKASVLGEAIRYLKQMEEKVSVLEEEQKRKKTVESVVIVKKSQLSINDAENSCSDTDSTFDETLPEIEARFCERSVLIRLHCLKSQGVIEKIMSEIEKLHLKVINSSSLTFGNFTLDITIIAQMDVEFCMTVKDLVRKLRSAYSSFM*