AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0787870

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0787870 MtrunA17_Chr5g0446411 96.562 349 12 0 1 349 1 349 0.0 691
Msa0787870 MtrunA17_Chr4g0076241 56.447 349 144 3 1 343 1 347 2.97e-131 379
Msa0787870 MtrunA17_Chr4g0051871 35.455 330 187 6 31 348 103 418 5.19e-63 206
Msa0787870 MtrunA17_Chr7g0270761 33.728 338 213 3 17 348 100 432 8.25e-63 206
Msa0787870 MtrunA17_Chr5g0443241 29.651 344 214 9 4 333 37 366 2.24e-44 156
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0787870 AT5G55630.2 66.337 303 102 0 47 349 56 358 9.91e-152 431
Msa0787870 AT5G55630.1 66.337 303 102 0 47 349 56 358 9.91e-152 431
Msa0787870 AT4G01840.1 33.908 348 200 6 15 343 67 403 3.80e-61 201
Msa0787870 AT4G18160.1 35.000 320 193 5 31 343 118 429 1.36e-56 190
Msa0787870 AT5G46370.1 36.331 278 170 1 73 343 152 429 5.49e-53 181
Msa0787870 AT1G02510.1 34.328 268 151 5 72 337 40 284 7.46e-48 163
Msa0787870 AT5G46360.1 35.714 154 99 0 195 348 106 259 1.06e-27 109
Msa0787870 AT5G46360.2 33.775 151 98 1 195 343 106 256 7.00e-22 94.0

Find 86 sgRNAs with CRISPR-Local

Find 235 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCCTTTCTGAGGCCATATT+TGG 0.140068 5_2:+74081498 Msa0787870:CDS
GACAAAAGGCACTTGTTAAA+TGG 0.156121 5_2:+74082147 Msa0787870:CDS
CAAACAATTAACCACGAATT+TGG 0.168279 5_2:-74083379 None:intergenic
ATAATCCTTATGGTCTATTT+AGG 0.214338 5_2:+74081552 Msa0787870:CDS
CTTAGTCAATGTAGAGAAAT+TGG 0.214466 5_2:+74081953 Msa0787870:CDS
CTTTAATCTTCTACCTTGTT+AGG 0.232655 5_2:+74081583 Msa0787870:CDS
GCTTTCTAAATTTATCTTCA+TGG 0.252407 5_2:+74081320 Msa0787870:exon
GAAGAATATTTGCAGTGTTT+TGG 0.270066 5_2:+74082057 Msa0787870:CDS
CACTTCATCATACTGGTATA+AGG 0.270152 5_2:-74083229 None:intergenic
TGTTGAACAAACACAGTAAA+AGG 0.292737 5_2:-74081988 None:intergenic
TGTTCGAGTCGTTCTAGTTT+TGG 0.293199 5_2:+74083096 Msa0787870:three_prime_UTR
TTCTGATTCAGTTGAAGTTT+TGG 0.296966 5_2:-74079809 None:intergenic
TGTCACAATTGTAAAATATA+AGG 0.307372 5_2:-74081646 None:intergenic
AAGAAAAGGTGCACATTTCC+TGG 0.317984 5_2:+74083165 Msa0787870:three_prime_UTR
GAAAATGACCAATTATGATT+TGG 0.325083 5_2:+74082181 Msa0787870:CDS
ACAATTACAACTCTTGGTTA+TGG 0.337875 5_2:+74082008 Msa0787870:CDS
TAGAACGGAGGAGCCTTCTT+TGG 0.342691 5_2:-74081419 None:intergenic
AAAAGTGGCTATAATCCTTA+TGG 0.344112 5_2:+74081542 Msa0787870:CDS
CCCTTTCTGAGGCCATATTT+GGG 0.348945 5_2:+74081499 Msa0787870:CDS
TGCAGCTTCCAAATCATAAT+TGG 0.349712 5_2:-74082189 None:intergenic
TTTCTCCGGAATGGCGTTGA+TGG 0.365822 5_2:+74081740 Msa0787870:CDS
CACATGAATGCATATGTCTA+TGG 0.371128 5_2:-74083282 None:intergenic
TGGATGTAGATGCCCAAATA+TGG 0.385877 5_2:-74081511 None:intergenic
ACAAAAGGCACTTGTTAAAT+GGG 0.414401 5_2:+74082148 Msa0787870:CDS
TAGTCCCATCAACGCCATTC+CGG 0.419499 5_2:-74081745 None:intergenic
TGTTCAACAATTACAACTCT+TGG 0.420374 5_2:+74082002 Msa0787870:CDS
AAGCAAAGCAGCAGATTACT+TGG 0.421571 5_2:+74081773 Msa0787870:CDS
TACTTTACATGCCAAATTCG+TGG 0.425279 5_2:+74083368 Msa0787870:three_prime_UTR
CCCAAATATGGCCTCAGAAA+GGG 0.440438 5_2:-74081499 None:intergenic
TTGTTAAATGGGTTCTTACA+AGG 0.443343 5_2:+74082159 Msa0787870:CDS
TTCTCCGGAATGGCGTTGAT+GGG 0.448679 5_2:+74081741 Msa0787870:CDS
GTCCTGTGATCTCAATAGTA+AGG 0.450755 5_2:-74081364 None:intergenic
TCACCTTCACTTCATCATAC+TGG 0.456747 5_2:-74083236 None:intergenic
ACAGGACACTGTGGCTCAAA+AGG 0.460014 5_2:+74081380 Msa0787870:CDS
TCGTTTATCAACAAAGGAGC+AGG 0.462707 5_2:-74081462 None:intergenic
AGTAATCTGCTGCTTTGCTT+AGG 0.463315 5_2:-74081770 None:intergenic
ACAATGACCACAGTTGGATA+TGG 0.469742 5_2:+74081666 Msa0787870:CDS
AGCTCCTTTAAAATTTCACT+TGG 0.474112 5_2:-74081852 None:intergenic
CCTTGATTTGGTTCCTAACA+AGG 0.475820 5_2:-74081596 None:intergenic
ATTTATAAGCTCAAAGAGAT+GGG 0.476286 5_2:+74082939 Msa0787870:CDS
AGATATAACACTTGTGATGA+AGG 0.479889 5_2:+74082977 Msa0787870:CDS
CCTTGTTAGGAACCAAATCA+AGG 0.487447 5_2:+74081596 Msa0787870:CDS
AAAAGTTTCTCAACTCAAGC+TGG 0.494527 5_2:+74082035 Msa0787870:CDS
GAGCTTGACATTGATCAGTC+TGG 0.496153 5_2:+74083008 Msa0787870:CDS
AAACACCGAAAACAGACAAA+AGG 0.497203 5_2:+74082133 Msa0787870:CDS
GACAAAATGCACAATGAGGA+TGG 0.505125 5_2:+74083127 Msa0787870:three_prime_UTR
TGTTTGTAAATGATCTACAT+TGG 0.506668 5_2:+74083396 Msa0787870:three_prime_UTR
TTGATGAGGATGGGACTGTT+GGG 0.510565 5_2:+74082216 Msa0787870:CDS
CTTATGGTCTATTTAGGTGT+AGG 0.511721 5_2:+74081558 Msa0787870:CDS
CTTGATGAGGATGGGACTGT+TGG 0.512385 5_2:+74082215 Msa0787870:CDS
TATTTATAAGCTCAAAGAGA+TGG 0.513290 5_2:+74082938 Msa0787870:CDS
GCTGCAGATCTTGATGAGGA+TGG 0.519988 5_2:+74082206 Msa0787870:CDS
GGGAGTTACTGAGTGTGGCC+TGG 0.523760 5_2:+74083259 Msa0787870:three_prime_UTR
CTACACCTAAATAGACCATA+AGG 0.525776 5_2:-74081557 None:intergenic
GCCCAAATATGGCCTCAGAA+AGG 0.525908 5_2:-74081500 None:intergenic
CTGCAGATCTTGATGAGGAT+GGG 0.535666 5_2:+74082207 Msa0787870:CDS
GCAAGAATCCGAGACTGTCC+AGG 0.536532 5_2:-74083183 None:intergenic
ATTGTGACAATGACCACAGT+TGG 0.542941 5_2:+74081660 Msa0787870:CDS
GCACATTTCCTGGACAGTCT+CGG 0.543326 5_2:+74083175 Msa0787870:three_prime_UTR
GCATGTAAAGTAAGTTGTGT+AGG 0.548110 5_2:-74083357 None:intergenic
CCAAATATGGCCTCAGAAAG+GGG 0.549928 5_2:-74081498 None:intergenic
TTGTAAAATATAAGGCATCA+AGG 0.551875 5_2:-74081638 None:intergenic
AAGGACGATTAAACCAAAGA+AGG 0.555389 5_2:+74081406 Msa0787870:CDS
GAATGCATATGTCTATGGCC+AGG 0.556545 5_2:-74083277 None:intergenic
TCTTTGTCGTTTATCAACAA+AGG 0.566507 5_2:-74081468 None:intergenic
CAGAGCTAGAATCACTTCAA+AGG 0.567928 5_2:+74079867 Msa0787870:five_prime_UTR
TGTTTAGTTCAGCCATGTAG+AGG 0.586266 5_2:-74082115 None:intergenic
TCCTGTGATCTCAATAGTAA+GGG 0.587155 5_2:-74081363 None:intergenic
TGTGAGGAGCACTTCTAGAA+CGG 0.588750 5_2:-74081434 None:intergenic
TATTGATTTGAAAACAAGCA+TGG 0.596597 5_2:-74083333 None:intergenic
GCCCTTACTATTGAGATCAC+AGG 0.597412 5_2:+74081362 Msa0787870:CDS
TAAGGTCACTATTGGGAACA+AGG 0.603841 5_2:-74081692 None:intergenic
ACTGTGGCTCAAAAGGCTAA+AGG 0.606518 5_2:+74081387 Msa0787870:CDS
CTTGTTAGGAACCAAATCAA+GGG 0.608102 5_2:+74081597 Msa0787870:CDS
ATACCAGTATGATGAAGTGA+AGG 0.631594 5_2:+74083233 Msa0787870:three_prime_UTR
GTATGATGAAGTGAAGGTGA+GGG 0.634988 5_2:+74083239 Msa0787870:three_prime_UTR
TTTATAAGCTCAAAGAGATG+GGG 0.636037 5_2:+74082940 Msa0787870:CDS
AGTATGATGAAGTGAAGGTG+AGG 0.641032 5_2:+74083238 Msa0787870:three_prime_UTR
GAGGAGCACTTCTAGAACGG+AGG 0.643797 5_2:-74081431 None:intergenic
AGAATCGATTCCCCTTTCTG+AGG 0.645736 5_2:+74081488 Msa0787870:CDS
CAATGACAAAATGCACAATG+AGG 0.649518 5_2:+74083123 Msa0787870:three_prime_UTR
GGTGAGGGAGTTACTGAGTG+TGG 0.670229 5_2:+74083254 Msa0787870:three_prime_UTR
GGAAGCTGCAGATCTTGATG+AGG 0.675047 5_2:+74082202 Msa0787870:CDS
AAAGGAGCAGGATCTGTGTG+AGG 0.683580 5_2:-74081450 None:intergenic
AAGGTCACCATATCCAACTG+TGG 0.725731 5_2:-74081673 None:intergenic
ATTGAGATCACAGGACACTG+TGG 0.729465 5_2:+74081371 Msa0787870:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTCAGTTTTTATAAAAT+GGG + chr5_2:74079995-74080014 Msa0787870:intron 10.0%
!!! CTTTTTCAGTTTTTATAAAA+TGG + chr5_2:74079994-74080013 Msa0787870:intron 15.0%
!! TGTGATAAACTAAATAAATA+AGG - chr5_2:74080144-74080163 None:intergenic 15.0%
!! AAAACATATATTTAAACCAT+TGG - chr5_2:74082867-74082886 None:intergenic 15.0%
!! TTCATTTATTTGAATATCTT+TGG + chr5_2:74082885-74082904 Msa0787870:intron 15.0%
!! GATAAACTAAATAAATAAGG+TGG - chr5_2:74080141-74080160 None:intergenic 20.0%
!!! TATATGGTTTTGTTCTTTTA+TGG + chr5_2:74080872-74080891 Msa0787870:intron 20.0%
!!! TATTCCTTTTTTTCAATTCA+AGG + chr5_2:74081007-74081026 Msa0787870:intron 20.0%
!!! AAAGGAATTTATTTTGTGTT+TGG + chr5_2:74081065-74081084 Msa0787870:intron 20.0%
!! ATTCAATTAGAAGTAAAACT+AGG + chr5_2:74081091-74081110 Msa0787870:intron 20.0%
!! TGTCACAATTGTAAAATATA+AGG - chr5_2:74081649-74081668 None:intergenic 20.0%
!! CGAAAAATAAAATTAGATGA+GGG - chr5_2:74082306-74082325 None:intergenic 20.0%
!! ACGAAAAATAAAATTAGATG+AGG - chr5_2:74082307-74082326 None:intergenic 20.0%
!! ATATGAAATGAAAGTTCATT+AGG - chr5_2:74082355-74082374 None:intergenic 20.0%
!!! AGTACTATTTTACTATGAAA+AGG - chr5_2:74082645-74082664 None:intergenic 20.0%
!! AGTTGAGTTACAAATTTATA+AGG + chr5_2:74082728-74082747 Msa0787870:intron 20.0%
!! GTTGAGTTACAAATTTATAA+GGG + chr5_2:74082729-74082748 Msa0787870:intron 20.0%
!!! AAGTCTGAAATTTTAAGAAA+AGG + chr5_2:74083151-74083170 Msa0787870:three_prime_UTR 20.0%
!! TAAACAAGTTCTCTTTTACT+TGG + chr5_2:74079768-74079787 Msa0787870:five_prime_UTR 25.0%
!!! GTTGTTTCTATTTTCCTTTA+TGG + chr5_2:74080017-74080036 Msa0787870:intron 25.0%
! ATCAAACAGACTAATTAACA+AGG - chr5_2:74080844-74080863 None:intergenic 25.0%
! TGTTTGATGTATGATCTATA+TGG + chr5_2:74080856-74080875 Msa0787870:intron 25.0%
! GCTTACATATTATGTTTCAA+AGG + chr5_2:74081047-74081066 Msa0787870:intron 25.0%
! GAAAGAAAAAAAGCTTAGTA+TGG - chr5_2:74081132-74081151 None:intergenic 25.0%
! CATATTACAGATCATCAAAA+GGG - chr5_2:74081162-74081181 None:intergenic 25.0%
! TTGTGTATATGTTGGAAATT+TGG + chr5_2:74081210-74081229 Msa0787870:intron 25.0%
! GCTTTCTAAATTTATCTTCA+TGG + chr5_2:74081320-74081339 Msa0787870:exon 25.0%
! ATAATCCTTATGGTCTATTT+AGG + chr5_2:74081552-74081571 Msa0787870:CDS 25.0%
!! TTGTAAAATATAAGGCATCA+AGG - chr5_2:74081641-74081660 None:intergenic 25.0%
! GAAAATGACCAATTATGATT+TGG + chr5_2:74082181-74082200 Msa0787870:CDS 25.0%
!!! ATTGCTCTAGTTTTTTTCTT+CGG + chr5_2:74082373-74082392 Msa0787870:intron 25.0%
! TATAGACTGTTAGATCATAT+TGG + chr5_2:74082445-74082464 Msa0787870:intron 25.0%
! TTGTGCTAAATATTGTAACA+TGG - chr5_2:74082480-74082499 None:intergenic 25.0%
! GATAAAATAGTGAAACTAAG+AGG - chr5_2:74082589-74082608 None:intergenic 25.0%
! TAAAAATAGAAGTCACAGAA+CGG - chr5_2:74082611-74082630 None:intergenic 25.0%
!!! TCTGTGACTTCTATTTTTAA+TGG + chr5_2:74082612-74082631 Msa0787870:intron 25.0%
! ACCTAACTCATTAGAATAAA+GGG - chr5_2:74082684-74082703 None:intergenic 25.0%
! TACCTAACTCATTAGAATAA+AGG - chr5_2:74082685-74082704 None:intergenic 25.0%
! TATTTATAAGCTCAAAGAGA+TGG + chr5_2:74082938-74082957 Msa0787870:CDS 25.0%
! ATTTATAAGCTCAAAGAGAT+GGG + chr5_2:74082939-74082958 Msa0787870:CDS 25.0%
!!! TATTGATTTGAAAACAAGCA+TGG - chr5_2:74083336-74083355 None:intergenic 25.0%
! TGTTTGTAAATGATCTACAT+TGG + chr5_2:74083396-74083415 Msa0787870:three_prime_UTR 25.0%
! TTCTGATTCAGTTGAAGTTT+TGG - chr5_2:74079812-74079831 None:intergenic 30.0%
AATCACTAACAACCAAAAAG+GGG - chr5_2:74079945-74079964 None:intergenic 30.0%
GAATCACTAACAACCAAAAA+GGG - chr5_2:74079946-74079965 None:intergenic 30.0%
TGAATCACTAACAACCAAAA+AGG - chr5_2:74079947-74079966 None:intergenic 30.0%
GCAAACAAAATCAACCATAA+AGG - chr5_2:74080034-74080053 None:intergenic 30.0%
! TTTCCACAATCATTTTCTAC+CGG - chr5_2:74080306-74080325 None:intergenic 30.0%
TAGAAAATGATTGTGGAAAG+TGG + chr5_2:74080307-74080326 Msa0787870:intron 30.0%
AGATCTAGAGATTAATGAGT+TGG + chr5_2:74080463-74080482 Msa0787870:intron 30.0%
TAAGTATTCTCTCCAATCAT+GGG - chr5_2:74080620-74080639 None:intergenic 30.0%
ATAAGTATTCTCTCCAATCA+TGG - chr5_2:74080621-74080640 None:intergenic 30.0%
GCATCAATTGAAATGAATGA+AGG + chr5_2:74080650-74080669 Msa0787870:intron 30.0%
AATGAAGGAATTGAACTGAA+AGG + chr5_2:74080665-74080684 Msa0787870:intron 30.0%
ATGAAGGAATTGAACTGAAA+GGG + chr5_2:74080666-74080685 Msa0787870:intron 30.0%
AACTGAAAGGGAAAAGAAAA+TGG + chr5_2:74080678-74080697 Msa0787870:intron 30.0%
CATGCCTTGAATTGAAAAAA+AGG - chr5_2:74081014-74081033 None:intergenic 30.0%
!!! CTTTTTTTCAATTCAAGGCA+TGG + chr5_2:74081012-74081031 Msa0787870:intron 30.0%
AACATAATATGTAAGCCATC+TGG - chr5_2:74081043-74081062 None:intergenic 30.0%
AAAAAGCTTAGTATGGTGAA+TGG - chr5_2:74081125-74081144 None:intergenic 30.0%
GCATATTACAGATCATCAAA+AGG - chr5_2:74081163-74081182 None:intergenic 30.0%
CCTTCAACGTATTGAAATTA+AGG - chr5_2:74081187-74081206 None:intergenic 30.0%
CCTTAATTTCAATACGTTGA+AGG + chr5_2:74081184-74081203 Msa0787870:intron 30.0%
! TCTTTGTCGTTTATCAACAA+AGG - chr5_2:74081471-74081490 None:intergenic 30.0%
!! ACATCCAAGCTTTAAAAAAG+TGG + chr5_2:74081527-74081546 Msa0787870:CDS 30.0%
!!! ATAGCCACTTTTTTAAAGCT+TGG - chr5_2:74081534-74081553 None:intergenic 30.0%
AAAAGTGGCTATAATCCTTA+TGG + chr5_2:74081542-74081561 Msa0787870:CDS 30.0%
CTTTAATCTTCTACCTTGTT+AGG + chr5_2:74081583-74081602 Msa0787870:CDS 30.0%
!! AGTTTTGTAAGGTCACTATT+GGG - chr5_2:74081702-74081721 None:intergenic 30.0%
!!! TAGTTTTGTAAGGTCACTAT+TGG - chr5_2:74081703-74081722 None:intergenic 30.0%
! TGGTCCAAGTGAAATTTTAA+AGG + chr5_2:74081848-74081867 Msa0787870:CDS 30.0%
!! AGCTCCTTTAAAATTTCACT+TGG - chr5_2:74081855-74081874 None:intergenic 30.0%
!! TCTTTTGATTCTCGTTATTG+CGG + chr5_2:74081920-74081939 Msa0787870:CDS 30.0%
CTTAGTCAATGTAGAGAAAT+TGG + chr5_2:74081953-74081972 Msa0787870:CDS 30.0%
TGTTGAACAAACACAGTAAA+AGG - chr5_2:74081991-74082010 None:intergenic 30.0%
TGTTCAACAATTACAACTCT+TGG + chr5_2:74082002-74082021 Msa0787870:CDS 30.0%
ACAATTACAACTCTTGGTTA+TGG + chr5_2:74082008-74082027 Msa0787870:CDS 30.0%
! GAAGAATATTTGCAGTGTTT+TGG + chr5_2:74082057-74082076 Msa0787870:CDS 30.0%
!! ACAAAAGGCACTTGTTAAAT+GGG + chr5_2:74082148-74082167 Msa0787870:CDS 30.0%
! TTGTTAAATGGGTTCTTACA+AGG + chr5_2:74082159-74082178 Msa0787870:CDS 30.0%
! TCGTTTAAGTTAAATGCCAA+AGG + chr5_2:74082323-74082342 Msa0787870:intron 30.0%
!!! TTGCTCTAGTTTTTTTCTTC+GGG + chr5_2:74082374-74082393 Msa0787870:intron 30.0%
! AGTCTATACCGATTATTAAC+AGG - chr5_2:74082433-74082452 None:intergenic 30.0%
ACTAAGAGGATTTATAAGCA+AGG - chr5_2:74082575-74082594 None:intergenic 30.0%
ACCCTTTATTCTAATGAGTT+AGG + chr5_2:74082680-74082699 Msa0787870:intron 30.0%
!!! TCTTTGGTTTTATTGTTCAC+AGG + chr5_2:74082901-74082920 Msa0787870:intron 30.0%
!!! CTTTGGTTTTATTGTTCACA+GGG + chr5_2:74082902-74082921 Msa0787870:intron 30.0%
TTTATAAGCTCAAAGAGATG+GGG + chr5_2:74082940-74082959 Msa0787870:CDS 30.0%
AGATATAACACTTGTGATGA+AGG + chr5_2:74082977-74082996 Msa0787870:CDS 30.0%
! CAATTTTGCGTGTATAATGT+TGG - chr5_2:74083078-74083097 None:intergenic 30.0%
CAAACAATTAACCACGAATT+TGG - chr5_2:74083382-74083401 None:intergenic 30.0%
! TGTTCAATTCATCCCCTTTT+TGG + chr5_2:74079930-74079949 Msa0787870:intron 35.0%
! TTTTTATTGAGTCGTGATCG+TGG + chr5_2:74080212-74080231 Msa0787870:intron 35.0%
! TTTTATTGAGTCGTGATCGT+GGG + chr5_2:74080213-74080232 Msa0787870:intron 35.0%
GGATGTGTGATAAGACTAAA+CGG + chr5_2:74080234-74080253 Msa0787870:intron 35.0%
GATGTGTGATAAGACTAAAC+GGG + chr5_2:74080235-74080254 Msa0787870:intron 35.0%
AAGGAGAGTAAATCAGATAG+AGG + chr5_2:74080385-74080404 Msa0787870:intron 35.0%
AGGAGAGTAAATCAGATAGA+GGG + chr5_2:74080386-74080405 Msa0787870:intron 35.0%
! ACGTATAGTTTTCCTTTGGA+GGG + chr5_2:74080591-74080610 Msa0787870:intron 35.0%
!! ATAGAAGTACTATGCAGTTG+AGG + chr5_2:74080783-74080802 Msa0787870:intron 35.0%
! TTATGGTATCTAGAGCTTGA+TGG + chr5_2:74080889-74080908 Msa0787870:intron 35.0%
! GAAGGCTTTTGTGTATATGT+TGG + chr5_2:74081202-74081221 Msa0787870:intron 35.0%
TCCATTATTATTTCGCTGAC+AGG + chr5_2:74081253-74081272 Msa0787870:intron 35.0%
CCATTATTATTTCGCTGACA+GGG + chr5_2:74081254-74081273 Msa0787870:intron 35.0%
AACTGCTTTGCATTTGAATC+AGG + chr5_2:74081285-74081304 Msa0787870:intron 35.0%
TCCTGTGATCTCAATAGTAA+GGG - chr5_2:74081366-74081385 None:intergenic 35.0%
AAGGACGATTAAACCAAAGA+AGG + chr5_2:74081406-74081425 Msa0787870:CDS 35.0%
CTACACCTAAATAGACCATA+AGG - chr5_2:74081560-74081579 None:intergenic 35.0%
CTTATGGTCTATTTAGGTGT+AGG + chr5_2:74081558-74081577 Msa0787870:CDS 35.0%
CTTGTTAGGAACCAAATCAA+GGG + chr5_2:74081597-74081616 Msa0787870:CDS 35.0%
! CTGTTTTCATTCCCTTGATT+TGG - chr5_2:74081611-74081630 None:intergenic 35.0%
! TTTCACTTGGACCAACTTTT+TGG - chr5_2:74081842-74081861 None:intergenic 35.0%
!! CTTTTGATTCTCGTTATTGC+GGG + chr5_2:74081921-74081940 Msa0787870:CDS 35.0%
!! TTTTGATTCTCGTTATTGCG+GGG + chr5_2:74081922-74081941 Msa0787870:CDS 35.0%
AAAAGTTTCTCAACTCAAGC+TGG + chr5_2:74082035-74082054 Msa0787870:CDS 35.0%
AAACACCGAAAACAGACAAA+AGG + chr5_2:74082133-74082152 Msa0787870:CDS 35.0%
! AAGTGCCTTTTGTCTGTTTT+CGG - chr5_2:74082141-74082160 None:intergenic 35.0%
!! GACAAAAGGCACTTGTTAAA+TGG + chr5_2:74082147-74082166 Msa0787870:CDS 35.0%
TGCAGCTTCCAAATCATAAT+TGG - chr5_2:74082192-74082211 None:intergenic 35.0%
GTTCATTAGGTTTAGACCTT+TGG - chr5_2:74082342-74082361 None:intergenic 35.0%
!!! TGCTCTAGTTTTTTTCTTCG+GGG + chr5_2:74082375-74082394 Msa0787870:intron 35.0%
ATATTTAGCACAAACCCGAT+TGG + chr5_2:74082487-74082506 Msa0787870:intron 35.0%
!! ATTTGACACAAACCGATTTG+TGG + chr5_2:74082808-74082827 Msa0787870:intron 35.0%
CAGTGAAAGGTAAAATGAGA+TGG + chr5_2:74082833-74082852 Msa0787870:intron 35.0%
TGAAAGGTAAAATGAGATGG+TGG + chr5_2:74082836-74082855 Msa0787870:intron 35.0%
GAAAGGTAAAATGAGATGGT+GGG + chr5_2:74082837-74082856 Msa0787870:intron 35.0%
CAATGACAAAATGCACAATG+AGG + chr5_2:74083123-74083142 Msa0787870:three_prime_UTR 35.0%
CACTTCATCATACTGGTATA+AGG - chr5_2:74083232-74083251 None:intergenic 35.0%
ATACCAGTATGATGAAGTGA+AGG + chr5_2:74083233-74083252 Msa0787870:three_prime_UTR 35.0%
CACATGAATGCATATGTCTA+TGG - chr5_2:74083285-74083304 None:intergenic 35.0%
GCATGTAAAGTAAGTTGTGT+AGG - chr5_2:74083360-74083379 None:intergenic 35.0%
TACTTTACATGCCAAATTCG+TGG + chr5_2:74083368-74083387 Msa0787870:three_prime_UTR 35.0%
CAGAGCTAGAATCACTTCAA+AGG + chr5_2:74079867-74079886 Msa0787870:five_prime_UTR 40.0%
TAGACATGACAACGTTAGAG+AGG + chr5_2:74080265-74080284 Msa0787870:intron 40.0%
AGACATGACAACGTTAGAGA+GGG + chr5_2:74080266-74080285 Msa0787870:intron 40.0%
! GAGAAGACTCGTAGATTTTG+TGG + chr5_2:74080361-74080380 Msa0787870:intron 40.0%
! GACTCGTAGATTTTGTGGTA+AGG + chr5_2:74080366-74080385 Msa0787870:intron 40.0%
GAGGGTAGTCAAATTACTAG+AGG + chr5_2:74080404-74080423 Msa0787870:intron 40.0%
CAAGTCCTATCCTACTAAGT+CGG - chr5_2:74080545-74080564 None:intergenic 40.0%
! CTCGACGTATAGTTTTCCTT+TGG + chr5_2:74080587-74080606 Msa0787870:intron 40.0%
! GACGTATAGTTTTCCTTTGG+AGG + chr5_2:74080590-74080609 Msa0787870:intron 40.0%
!! CGTATAGTTTTCCTTTGGAG+GGG + chr5_2:74080592-74080611 Msa0787870:intron 40.0%
! TCACGTGGTGATTTGCAAAT+AGG + chr5_2:74080814-74080833 Msa0787870:intron 40.0%
! TAGTATGGTGAATGGTGATG+AGG - chr5_2:74081117-74081136 None:intergenic 40.0%
CCCTGTCAGCGAAATAATAA+TGG - chr5_2:74081257-74081276 None:intergenic 40.0%
!! GGGCTCATTTTTATCATTGC+TGG - chr5_2:74081346-74081365 None:intergenic 40.0%
GTCCTGTGATCTCAATAGTA+AGG - chr5_2:74081367-74081386 None:intergenic 40.0%
TCGTTTATCAACAAAGGAGC+AGG - chr5_2:74081465-74081484 None:intergenic 40.0%
TGGATGTAGATGCCCAAATA+TGG - chr5_2:74081514-74081533 None:intergenic 40.0%
!! CCTTGATTTGGTTCCTAACA+AGG - chr5_2:74081599-74081618 None:intergenic 40.0%
CCTTGTTAGGAACCAAATCA+AGG + chr5_2:74081596-74081615 Msa0787870:CDS 40.0%
ATTGTGACAATGACCACAGT+TGG + chr5_2:74081660-74081679 Msa0787870:CDS 40.0%
ACAATGACCACAGTTGGATA+TGG + chr5_2:74081666-74081685 Msa0787870:CDS 40.0%
TAAGGTCACTATTGGGAACA+AGG - chr5_2:74081695-74081714 None:intergenic 40.0%
!! AGTAATCTGCTGCTTTGCTT+AGG - chr5_2:74081773-74081792 None:intergenic 40.0%
AAGCAAAGCAGCAGATTACT+TGG + chr5_2:74081773-74081792 Msa0787870:CDS 40.0%
!! GCAGTGTTTTGGATACTAAC+AGG + chr5_2:74082068-74082087 Msa0787870:CDS 40.0%
! AGCTCAGTTTTTCCTCTACA+TGG + chr5_2:74082103-74082122 Msa0787870:CDS 40.0%
! TGTTTAGTTCAGCCATGTAG+AGG - chr5_2:74082118-74082137 None:intergenic 40.0%
TGTGGCGACCTGTTAATAAT+CGG + chr5_2:74082422-74082441 Msa0787870:intron 40.0%
! CCATTAGGCTTTTGACCTTT+TGG - chr5_2:74082541-74082560 None:intergenic 40.0%
CCAAAAGGTCAAAAGCCTAA+TGG + chr5_2:74082538-74082557 Msa0787870:intron 40.0%
TTATAAGCAAGGTGAGGTGA+AGG - chr5_2:74082564-74082583 None:intergenic 40.0%
GAGGATTTATAAGCAAGGTG+AGG - chr5_2:74082570-74082589 None:intergenic 40.0%
CCTTTCACTGTTCCACAAAT+CGG - chr5_2:74082823-74082842 None:intergenic 40.0%
!!! TGTTCGAGTCGTTCTAGTTT+TGG + chr5_2:74083096-74083115 Msa0787870:three_prime_UTR 40.0%
GACAAAATGCACAATGAGGA+TGG + chr5_2:74083127-74083146 Msa0787870:three_prime_UTR 40.0%
! AAGAAAAGGTGCACATTTCC+TGG + chr5_2:74083165-74083184 Msa0787870:three_prime_UTR 40.0%
TCACCTTCACTTCATCATAC+TGG - chr5_2:74083239-74083258 None:intergenic 40.0%
AGTATGATGAAGTGAAGGTG+AGG + chr5_2:74083238-74083257 Msa0787870:three_prime_UTR 40.0%
GTATGATGAAGTGAAGGTGA+GGG + chr5_2:74083239-74083258 Msa0787870:three_prime_UTR 40.0%
ATGACAACGTTAGAGAGGGT+TGG + chr5_2:74080270-74080289 Msa0787870:intron 45.0%
TGACAACGTTAGAGAGGGTT+GGG + chr5_2:74080271-74080290 Msa0787870:intron 45.0%
! CCACAATCATTTTCTACCGG+AGG - chr5_2:74080303-74080322 None:intergenic 45.0%
CCTCCGGTAGAAAATGATTG+TGG + chr5_2:74080300-74080319 Msa0787870:intron 45.0%
ACTAAGTCGGATCGACTACA+TGG - chr5_2:74080532-74080551 None:intergenic 45.0%
TGTAGTCGATCCGACTTAGT+AGG + chr5_2:74080532-74080551 Msa0787870:intron 45.0%
TCGATCCGACTTAGTAGGAT+AGG + chr5_2:74080537-74080556 Msa0787870:intron 45.0%
CGACTTAGTAGGATAGGACT+TGG + chr5_2:74080543-74080562 Msa0787870:intron 45.0%
AGTACTATGCAGTTGAGGCA+AGG + chr5_2:74080788-74080807 Msa0787870:intron 45.0%
!! AGAGCTTGATGGTGCTTCAA+TGG + chr5_2:74080900-74080919 Msa0787870:intron 45.0%
CAAGGCATGGAATATCCAGA+TGG + chr5_2:74081025-74081044 Msa0787870:intron 45.0%
GCCCTTACTATTGAGATCAC+AGG + chr5_2:74081362-74081381 Msa0787870:CDS 45.0%
ATTGAGATCACAGGACACTG+TGG + chr5_2:74081371-74081390 Msa0787870:CDS 45.0%
ACTGTGGCTCAAAAGGCTAA+AGG + chr5_2:74081387-74081406 Msa0787870:CDS 45.0%
!! TGTGAGGAGCACTTCTAGAA+CGG - chr5_2:74081437-74081456 None:intergenic 45.0%
! AGAATCGATTCCCCTTTCTG+AGG + chr5_2:74081488-74081507 Msa0787870:CDS 45.0%
CCAAATATGGCCTCAGAAAG+GGG - chr5_2:74081501-74081520 None:intergenic 45.0%
CCCAAATATGGCCTCAGAAA+GGG - chr5_2:74081502-74081521 None:intergenic 45.0%
!! CCCTTTCTGAGGCCATATTT+GGG + chr5_2:74081499-74081518 Msa0787870:CDS 45.0%
AAGGTCACCATATCCAACTG+TGG - chr5_2:74081676-74081695 None:intergenic 45.0%
!!! CGCAAGCGAGTAGTTTTGTA+AGG - chr5_2:74081713-74081732 None:intergenic 45.0%
!!! GCTTGCGCTTTTGTTTTCTC+CGG + chr5_2:74081726-74081745 Msa0787870:CDS 45.0%
!!! CGCTTTTGTTTTCTCCGGAA+TGG + chr5_2:74081731-74081750 Msa0787870:CDS 45.0%
!! AACTTTTTGGCGCATGTGCA+TGG - chr5_2:74081829-74081848 None:intergenic 45.0%
ATGCACATGCGCCAAAAAGT+TGG + chr5_2:74081828-74081847 Msa0787870:CDS 45.0%
CTGCAGATCTTGATGAGGAT+GGG + chr5_2:74082207-74082226 Msa0787870:CDS 45.0%
TTGATGAGGATGGGACTGTT+GGG + chr5_2:74082216-74082235 Msa0787870:CDS 45.0%
TTTAGCACAAACCCGATTGG+AGG + chr5_2:74082490-74082509 Msa0787870:intron 45.0%
CTCTAAACACTCCTCCAATC+GGG - chr5_2:74082504-74082523 None:intergenic 45.0%
CCTCTAAACACTCCTCCAAT+CGG - chr5_2:74082505-74082524 None:intergenic 45.0%
!! ATTGGAGGAGTGTTTAGAGG+AGG + chr5_2:74082505-74082524 Msa0787870:intron 45.0%
!! TTGGAGGAGTGTTTAGAGGA+GGG + chr5_2:74082506-74082525 Msa0787870:intron 45.0%
GGAGGGATTCATGATCCAAA+AGG + chr5_2:74082523-74082542 Msa0787870:intron 45.0%
! CCGATTTGTGGAACAGTGAA+AGG + chr5_2:74082820-74082839 Msa0787870:intron 45.0%
GAGCTTGACATTGATCAGTC+TGG + chr5_2:74083008-74083027 Msa0787870:CDS 45.0%
GAATGCATATGTCTATGGCC+AGG - chr5_2:74083280-74083299 None:intergenic 45.0%
GACAACGTTAGAGAGGGTTG+GGG + chr5_2:74080272-74080291 Msa0787870:intron 50.0%
GTTGAGGCAAGGAAATCACG+TGG + chr5_2:74080799-74080818 Msa0787870:intron 50.0%
ACAGGACACTGTGGCTCAAA+AGG + chr5_2:74081380-74081399 Msa0787870:CDS 50.0%
TAGAACGGAGGAGCCTTCTT+TGG - chr5_2:74081422-74081441 None:intergenic 50.0%
AAAGGAGCAGGATCTGTGTG+AGG - chr5_2:74081453-74081472 None:intergenic 50.0%
!! CCCCTTTCTGAGGCCATATT+TGG + chr5_2:74081498-74081517 Msa0787870:CDS 50.0%
GCCCAAATATGGCCTCAGAA+AGG - chr5_2:74081503-74081522 None:intergenic 50.0%
! TTTCTCCGGAATGGCGTTGA+TGG + chr5_2:74081740-74081759 Msa0787870:CDS 50.0%
!! TTCTCCGGAATGGCGTTGAT+GGG + chr5_2:74081741-74081760 Msa0787870:CDS 50.0%
TAGTCCCATCAACGCCATTC+CGG - chr5_2:74081748-74081767 None:intergenic 50.0%
GGAAGCTGCAGATCTTGATG+AGG + chr5_2:74082202-74082221 Msa0787870:CDS 50.0%
GCTGCAGATCTTGATGAGGA+TGG + chr5_2:74082206-74082225 Msa0787870:CDS 50.0%
CTTGATGAGGATGGGACTGT+TGG + chr5_2:74082215-74082234 Msa0787870:CDS 50.0%
!! CCGATTGGAGGAGTGTTTAG+AGG + chr5_2:74082502-74082521 Msa0787870:intron 50.0%
AAGGTGAGGTGAAGGCCATT+AGG - chr5_2:74082556-74082575 None:intergenic 50.0%
!! TGAGATGGTGGGAGTACCAA+TGG + chr5_2:74082848-74082867 Msa0787870:intron 50.0%
GCACATTTCCTGGACAGTCT+CGG + chr5_2:74083175-74083194 Msa0787870:three_prime_UTR 50.0%
AATCATGGGCACCCCTCCAA+AGG - chr5_2:74080606-74080625 None:intergenic 55.0%
!! TTTGGAGGGGTGCCCATGAT+TGG + chr5_2:74080605-74080624 Msa0787870:intron 55.0%
!! GAGGAGCACTTCTAGAACGG+AGG - chr5_2:74081434-74081453 None:intergenic 55.0%
TCTTCGGGGCTTGTCCTTGA+CGG + chr5_2:74082389-74082408 Msa0787870:intron 55.0%
GGCTTGTCCTTGACGGAGTT+AGG + chr5_2:74082396-74082415 Msa0787870:intron 55.0%
CACACTGCCTAACTCCGTCA+AGG - chr5_2:74082406-74082425 None:intergenic 55.0%
CTTGACGGAGTTAGGCAGTG+TGG + chr5_2:74082404-74082423 Msa0787870:intron 55.0%
GCAAGAATCCGAGACTGTCC+AGG - chr5_2:74083186-74083205 None:intergenic 55.0%
GGTGAGGGAGTTACTGAGTG+TGG + chr5_2:74083254-74083273 Msa0787870:three_prime_UTR 55.0%
GGGAGTTACTGAGTGTGGCC+TGG + chr5_2:74083259-74083278 Msa0787870:three_prime_UTR 60.0%
GAGGGTTGGGGTAGCACCTC+CGG + chr5_2:74080284-74080303 Msa0787870:intron 65.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 74079745 74083447 74079745 ID=Msa0787870;Name=Msa0787870
chr5_2 mRNA 74079745 74083447 74079745 ID=Msa0787870-mRNA-1;Parent=Msa0787870;Name=Msa0787870-mRNA-1;_AED=0.00;_eAED=0.00;_QI=176|1|1|1|0.5|0.33|3|374|349
chr5_2 exon 74079745 74079888 74079745 ID=Msa0787870-mRNA-1:exon:22564;Parent=Msa0787870-mRNA-1
chr5_2 exon 74081307 74082237 74081307 ID=Msa0787870-mRNA-1:exon:22565;Parent=Msa0787870-mRNA-1
chr5_2 exon 74082923 74083447 74082923 ID=Msa0787870-mRNA-1:exon:22566;Parent=Msa0787870-mRNA-1
chr5_2 five_prime_UTR 74079745 74079888 74079745 ID=Msa0787870-mRNA-1:five_prime_utr;Parent=Msa0787870-mRNA-1
chr5_2 five_prime_UTR 74081307 74081338 74081307 ID=Msa0787870-mRNA-1:five_prime_utr;Parent=Msa0787870-mRNA-1
chr5_2 CDS 74081339 74082237 74081339 ID=Msa0787870-mRNA-1:cds;Parent=Msa0787870-mRNA-1
chr5_2 CDS 74082923 74083073 74082923 ID=Msa0787870-mRNA-1:cds;Parent=Msa0787870-mRNA-1
chr5_2 three_prime_UTR 74083074 74083447 74083074 ID=Msa0787870-mRNA-1:three_prime_utr;Parent=Msa0787870-mRNA-1
Gene Sequence

>Msa0787870

ATGGCCAGCAATGATAAAAATGAGCCCTTACTATTGAGATCACAGGACACTGTGGCTCAAAAGGCTAAAGGACGATTAAACCAAAGAAGGCTCCTCCGTTCTAGAAGTGCTCCTCACACAGATCCTGCTCCTTTGTTGATAAACGACAAAGAATCGATTCCCCTTTCTGAGGCCATATTTGGGCATCTACATCCAAGCTTTAAAAAAGTGGCTATAATCCTTATGGTCTATTTAGGTGTAGGAACTTTAATCTTCTACCTTGTTAGGAACCAAATCAAGGGAATGAAAACAGAGAGATTCCTTGATGCCTTATATTTTACAATTGTGACAATGACCACAGTTGGATATGGTGACCTTGTTCCCAATAGTGACCTTACAAAACTACTCGCTTGCGCTTTTGTTTTCTCCGGAATGGCGTTGATGGGACTAATCCTAAGCAAAGCAGCAGATTACTTGGTTGAGAAGCAAGAAGTTTTGTTAATTAAAGCCATGCACATGCGCCAAAAAGTTGGTCCAAGTGAAATTTTAAAGGAGCTTGAGATCAATAAAACAAGATACAAATTTTTTCTTGTCTTTTTTCTTCTTTTGATTCTCGTTATTGCGGGGACAATCTTCTTAGTCAATGTAGAGAAATTGGATGTTATTGATGCCTTTTACTGTGTTTGTTCAACAATTACAACTCTTGGTTATGGAGATAAAAGTTTCTCAACTCAAGCTGGAAGAATATTTGCAGTGTTTTGGATACTAACAGGTACTGTTTGCTTAGCTCAGTTTTTCCTCTACATGGCTGAACTAAACACCGAAAACAGACAAAAGGCACTTGTTAAATGGGTTCTTACAAGGAAAATGACCAATTATGATTTGGAAGCTGCAGATCTTGATGAGGATGGGACTGTTGGGGCTGCTGAATTTGTTATTTATAAGCTCAAAGAGATGGGGAAGATTAGTCAAGAAGATATAACACTTGTGATGAAGGAGTTTGAAGAGCTTGACATTGATCAGTCTGGTACACTGTCTGTTTCTGATATAACACTTGCTCAATCGTCTTAA
Protein sequence

>Msa0787870

MASNDKNEPLLLRSQDTVAQKAKGRLNQRRLLRSRSAPHTDPAPLLINDKESIPLSEAIFGHLHPSFKKVAIILMVYLGVGTLIFYLVRNQIKGMKTERFLDALYFTIVTMTTVGYGDLVPNSDLTKLLACAFVFSGMALMGLILSKAADYLVEKQEVLLIKAMHMRQKVGPSEILKELEINKTRYKFFLVFFLLLILVIAGTIFLVNVEKLDVIDAFYCVCSTITTLGYGDKSFSTQAGRIFAVFWILTGTVCLAQFFLYMAELNTENRQKALVKWVLTRKMTNYDLEAADLDEDGTVGAAEFVIYKLKEMGKISQEDITLVMKEFEELDIDQSGTLSVSDITLAQSS*