AlfalfaGEDB | Msa0815590

M. sativa cultivar zhongmu-4 / Msa0815590

KEGG: -
GO: GO:0005575 GO:0005618 GO:0005623 GO:0016020 GO:0030312 GO:0044464 GO:0071944
PFAM: PF03330.21

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0815590	XP_003616175.1	97.368	266	7	0	1	266	1	266	0.0	544

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0815590	sp\|Q9M9P0\|EXP13_ARATH	83.628	226	37	0	40	265	39	264	6.19e-145	409

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0815590	G7KFU6	97.368	266	7	0	1	266	1	266	0.0	544

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0097120	Msa0815590	0.807385	5.187253e-50	-8.615850e-47
Msa0736500	Msa0815590	0.892162	2.047403e-74	-8.615850e-47
Msa0815590	Msa1060300	0.805324	1.410669e-49	-8.615850e-47
Msa0815590	Msa1383780	0.800928	1.145054e-48	-8.615850e-47
Msa0815590	Msa1427180	-0.801668	8.076511e-49	-8.615850e-47
Msa0815590	Msa1460970	0.808202	3.476940e-50	-8.615850e-47
Msa0186670	Msa0815590	0.816759	4.685761e-52	-8.615850e-47
Msa0385330	Msa0815590	0.804559	2.038509e-49	-8.615850e-47
Msa0438320	Msa0815590	0.817092	3.945765e-52	-8.615850e-47
Msa0462980	Msa0815590	0.801562	8.490251e-49	-8.615850e-47
Msa0508580	Msa0815590	0.802865	4.580650e-49	-8.615850e-47
Msa0523070	Msa0815590	0.805921	1.056674e-49	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0815590	MtrunA17_Chr5g0433841	97.368	266	7	0	1	266	1	266	0.0	544
Msa0815590	MtrunA17_Chr3g0109801	68.846	260	68	5	18	264	25	284	3.11e-128	365
Msa0815590	MtrunA17_Chr4g0021561	53.962	265	105	2	1	264	8	256	4.26e-99	290
Msa0815590	MtrunA17_Chr5g0435311	56.000	225	98	1	41	264	82	306	4.42e-91	271
Msa0815590	MtrunA17_Chr5g0417701	56.696	224	96	1	41	264	25	247	2.20e-90	268
Msa0815590	MtrunA17_Chr4g0042521	55.357	224	99	1	41	264	25	247	2.74e-89	265
Msa0815590	MtrunA17_Chr3g0092071	56.250	224	97	1	41	264	25	247	7.46e-89	264
Msa0815590	MtrunA17_Chr7g0238681	49.805	257	124	2	9	264	4	256	1.89e-88	263
Msa0815590	MtrunA17_Chr3g0128801	54.018	224	102	1	41	264	31	253	5.39e-88	262
Msa0815590	MtrunA17_Chr3g0128791	54.018	224	102	1	41	264	31	253	1.44e-87	261
Msa0815590	MtrunA17_Chr2g0328261	54.667	225	101	1	41	264	34	258	2.40e-87	260
Msa0815590	MtrunA17_Chr5g0432611	53.947	228	104	1	37	264	25	251	2.68e-87	260
Msa0815590	MtrunA17_Chr3g0107311	54.911	224	100	1	41	264	32	254	1.14e-86	258
Msa0815590	MtrunA17_Chr7g0217661	52.863	227	98	2	40	266	25	242	1.38e-85	255
Msa0815590	MtrunA17_Chr3g0107291	52.361	233	110	1	32	264	29	260	3.14e-85	255
Msa0815590	MtrunA17_Chr4g0031621	55.310	226	100	1	40	264	31	256	1.88e-81	245
Msa0815590	MtrunA17_Chr7g0218751	52.679	224	103	2	40	262	27	248	2.46e-80	242
Msa0815590	MtrunA17_Chr8g0360041	51.101	227	108	2	36	261	26	250	1.46e-78	238
Msa0815590	MtrunA17_Chr1g0193631	50.667	225	108	2	41	262	30	254	6.30e-77	234
Msa0815590	MtrunA17_Chr7g0218761	54.412	204	90	2	60	262	8	209	6.80e-76	229
Msa0815590	MtrunA17_Chr4g0069711	43.525	278	138	5	3	263	8	283	8.05e-76	232
Msa0815590	MtrunA17_Chr7g0218741	51.327	226	106	3	40	263	28	251	1.95e-75	230
Msa0815590	MtrunA17_Chr6g0453341	53.097	226	97	2	41	266	38	254	2.00e-74	227
Msa0815590	MtrunA17_Chr1g0157061	50.000	212	104	1	53	262	27	238	2.07e-73	224
Msa0815590	MtrunA17_Chr7g0271001	47.982	223	113	2	41	260	27	249	1.10e-71	220
Msa0815590	MtrunA17_Chr8g0360721	52.475	202	93	2	60	260	17	216	6.15e-71	217
Msa0815590	MtrunA17_Chr5g0398271	45.333	225	122	1	39	262	35	259	1.18e-66	207
Msa0815590	MtrunA17_Chr4g0057091	45.852	229	119	2	33	260	32	256	9.12e-64	200
Msa0815590	MtrunA17_Chr2g0324181	66.337	101	34	0	164	264	17	117	5.39e-46	150
Msa0815590	MtrunA17_Chr2g0324191	42.424	165	79	2	1	164	3	152	5.23e-41	138
Msa0815590	MtrunA17_Chr4g0022841	53.043	115	54	0	147	261	2	116	2.14e-37	128
Msa0815590	MtrunA17_Chr4g0022851	51.456	103	45	2	36	137	27	125	7.26e-29	106
Msa0815590	MtrunA17_Chr2g0324221	28.390	236	144	7	39	262	33	255	1.90e-23	95.9
Msa0815590	MtrunA17_Chr2g0324201	29.362	235	141	8	40	262	32	253	2.19e-22	93.2
Msa0815590	MtrunA17_Chr4g0021641	30.213	235	139	8	40	262	40	261	2.30e-22	93.2
Msa0815590	MtrunA17_Chr4g0012801	30.833	240	124	11	41	260	42	259	5.67e-22	92.0
Msa0815590	MtrunA17_Chr8g0359971	53.947	76	34	1	186	260	2	77	7.78e-21	84.0
Msa0815590	MtrunA17_Chr7g0231221	29.565	230	133	11	46	259	53	269	2.28e-18	82.4
Msa0815590	MtrunA17_Chr7g0231211	28.755	233	147	10	37	260	43	265	1.19e-17	80.5
Msa0815590	MtrunA17_Chr8g0359981	44.828	87	36	4	83	164	2	81	7.00e-16	72.0
Msa0815590	MtrunA17_Chr4g0038741	26.724	232	136	9	44	260	26	238	2.75e-13	68.2
Msa0815590	MtrunA17_Chr1g0193571	52.727	55	26	0	208	262	3	57	3.05e-12	60.5
Msa0815590	MtrunA17_Chr5g0399811	27.273	231	149	10	44	265	32	252	7.31e-12	63.9
Msa0815590	MtrunA17_Chr4g0055561	26.695	236	150	9	34	260	24	245	9.25e-11	60.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0815590	AT3G03220.1	83.628	226	37	0	40	265	39	264	6.29e-146	409
Msa0815590	AT5G56320.1	58.036	224	93	1	41	264	29	251	2.73e-93	276
Msa0815590	AT5G56320.2	58.036	224	93	1	41	264	29	251	2.73e-93	276
Msa0815590	AT5G56320.3	58.036	224	93	1	41	264	29	251	7.83e-93	276
Msa0815590	AT3G55500.1	56.637	226	97	1	40	264	33	258	2.99e-91	271
Msa0815590	AT5G05290.1	57.778	225	93	2	41	264	32	255	4.66e-91	270
Msa0815590	AT2G39700.1	50.758	264	114	2	2	264	7	255	5.56e-91	270
Msa0815590	AT5G02260.1	54.667	225	101	1	41	264	32	256	4.72e-90	267
Msa0815590	AT1G69530.2	51.136	264	114	3	1	264	1	249	4.91e-90	267
Msa0815590	AT1G69530.1	51.136	264	114	3	1	264	1	249	4.91e-90	267
Msa0815590	AT2G37640.1	56.000	225	98	1	41	264	36	260	1.07e-89	267
Msa0815590	AT3G29030.1	54.867	226	94	1	41	266	38	255	1.58e-88	263
Msa0815590	AT2G03090.1	56.250	224	97	1	41	264	30	252	2.61e-88	263
Msa0815590	AT1G69530.3	51.351	259	111	3	1	259	1	244	6.53e-88	263
Msa0815590	AT2G40610.1	52.444	225	105	2	41	264	30	253	1.03e-84	254
Msa0815590	AT1G26770.1	55.357	224	99	1	41	264	26	248	1.73e-84	253
Msa0815590	AT1G26770.2	55.357	224	99	1	41	264	36	258	2.59e-84	253
Msa0815590	AT2G37640.2	57.212	208	88	1	58	264	28	235	3.32e-84	252
Msa0815590	AT5G39270.1	51.261	238	113	2	25	262	42	276	2.82e-81	246
Msa0815590	AT2G28950.1	53.778	225	103	1	41	264	31	255	4.01e-81	244
Msa0815590	AT5G39290.1	51.931	233	106	3	30	262	50	276	1.32e-80	244
Msa0815590	AT5G39280.1	52.232	224	104	2	39	262	52	272	1.44e-79	241
Msa0815590	AT5G39300.1	46.970	264	128	4	8	262	13	273	1.68e-79	241
Msa0815590	AT4G38210.1	51.316	228	108	2	40	264	27	254	7.16e-79	239
Msa0815590	AT1G20190.1	49.091	220	110	1	43	260	28	247	1.08e-75	231
Msa0815590	AT1G69530.4	45.455	264	99	4	1	264	1	219	7.70e-73	222
Msa0815590	AT1G62980.1	48.444	225	115	1	39	262	29	253	1.93e-72	223
Msa0815590	AT4G01630.1	49.107	224	109	3	41	260	28	250	2.78e-71	219
Msa0815590	AT1G69530.5	45.560	259	96	4	1	259	1	214	6.21e-71	218
Msa0815590	AT5G39310.1	45.306	245	130	3	19	262	68	309	4.00e-69	216
Msa0815590	AT1G12560.1	42.308	260	133	4	2	260	13	256	8.25e-69	213
Msa0815590	AT5G39260.1	48.444	225	107	3	39	262	43	259	4.93e-66	206
Msa0815590	AT3G15370.1	47.414	232	107	6	41	264	27	251	1.63e-62	197
Msa0815590	AT1G20190.2	48.128	187	95	1	76	260	2	188	2.19e-62	195
Msa0815590	AT3G15370.2	47.907	215	97	6	58	264	74	281	1.01e-57	186
Msa0815590	AT2G20750.1	31.250	256	150	9	22	262	20	264	2.12e-24	99.0
Msa0815590	AT4G28250.1	31.250	240	137	9	37	262	32	257	5.18e-24	97.8
Msa0815590	AT4G28250.2	30.962	239	133	9	37	262	32	251	8.90e-22	91.7
Msa0815590	AT1G65680.1	28.512	242	145	9	34	260	40	268	4.99e-19	84.7
Msa0815590	AT2G20750.2	30.412	194	113	6	22	203	20	203	5.92e-15	72.4
Msa0815590	AT1G65681.1	26.941	219	130	8	57	260	14	217	8.42e-14	69.3
Msa0815590	AT4G17030.1	26.778	239	137	9	40	260	26	244	3.62e-13	67.8
Msa0815590	AT3G60570.2	24.424	217	137	8	59	262	4	206	6.34e-11	60.8
Msa0815590	AT4G28250.4	31.073	177	94	7	37	203	32	190	8.61e-11	60.5

Find 110 sgRNAs with CRISPR-Local

Find 256 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AATTTCTGTGCGCCGAATTA+TGG	0.162915	5_3:-49225443	Msa0815590:CDS
GGCTTTCAATGGGTCGGAAT+TGG	0.242564	5_3:-49223688	Msa0815590:CDS
GCTTTCAATGGGTCGGAATT+GGG	0.256772	5_3:-49223687	Msa0815590:CDS
GGAAGAACAAAGTGCTTATT+AGG	0.266775	5_3:+49225386	None:intergenic
TTGTCCAAAACTCCAGTCTT+TGG	0.295901	5_3:+49223565	None:intergenic
GCTCCCAAAGACTGGAGTTT+TGG	0.308877	5_3:-49223569	Msa0815590:CDS
GTAAGTAGACCTATAATATT+GGG	0.325816	5_3:+49223441	None:intergenic
GGTAAGTAGACCTATAATAT+TGG	0.326700	5_3:+49223440	None:intergenic
AACTTTCCATCTATTATCTT+AGG	0.332869	5_3:-49223315	Msa0815590:three_prime_UTR
ACGCGTTTATATGCCAGTTT+TGG	0.342695	5_3:+49223663	None:intergenic
CATTGTTGCGGTGAAGGTTA+AGG	0.343774	5_3:-49223726	Msa0815590:CDS
ATTTCTGTGCGCCGAATTAT+GGG	0.345552	5_3:-49225442	Msa0815590:CDS
GATTTAAAGTGGTGTATTCC+TGG	0.357927	5_3:-49225491	Msa0815590:CDS
TGTCCAAAACTCCAGTCTTT+GGG	0.365496	5_3:+49223566	None:intergenic
GTCGGAATTGGGGCCAAAAC+TGG	0.376301	5_3:-49223676	Msa0815590:CDS
TGCGCCGAATTATGGGTTTA+CGG	0.384118	5_3:-49225435	Msa0815590:CDS
AAGAACAGGTTGGCTTTCAA+TGG	0.397007	5_3:-49223699	Msa0815590:CDS
TAAACTCCTAAGATAATAGA+TGG	0.397206	5_3:+49223309	None:intergenic
GAATCAACCTCTTTCATTTG+AGG	0.401927	5_3:-49223633	Msa0815590:CDS
AACAGTTGCCATGCCGTAAC+CGG	0.402191	5_3:+49225583	None:intergenic
GTGGCGCGTGCTTTGAATTA+CGG	0.402202	5_3:-49225523	Msa0815590:CDS
AGAGACTGGTCCCTTGAAAC+AGG	0.404717	5_3:+49223494	None:intergenic
TATGAAAATATATACCTTGT+TGG	0.406110	5_3:-49223283	Msa0815590:three_prime_UTR
AATTATGGGTTTACGGTTGA+AGG	0.412698	5_3:-49225428	Msa0815590:CDS
ATTGTTGCGGTGAAGGTTAA+GGG	0.417256	5_3:-49223725	Msa0815590:CDS
AGAGTAGTTGATAATTGATT+TGG	0.424966	5_3:-49223368	Msa0815590:three_prime_UTR
CGATTTACAGTCACCGGATC+TGG	0.430139	5_3:-49223794	Msa0815590:CDS
GACTTATCAAAAGCCGGTTA+CGG	0.433896	5_3:-49225596	Msa0815590:CDS
GAGAAAATGAAGAAAGAAAG+TGG	0.437740	5_3:+49225878	None:intergenic
AATATTATAGGTCTACTTAC+CGG	0.448816	5_3:-49223438	Msa0815590:three_prime_UTR
ATCAAATTGCTTGCCTTCAA+AGG	0.451831	5_3:+49223541	None:intergenic
ACTGTGTCACGTGGATCTAC+CGG	0.453305	5_3:+49225641	None:intergenic
GTCGGCGGCGCGTGTGGCTA+CGG	0.454653	5_3:-49225620	Msa0815590:CDS
GTGCTGATCAGCAATGTTGC+TGG	0.456538	5_3:-49223758	Msa0815590:CDS
CAGATCCGGTGACTGTAAAT+CGG	0.457752	5_3:+49223795	None:intergenic
AACGAAGAAAACCTGTTTCA+AGG	0.458176	5_3:-49223505	Msa0815590:three_prime_UTR
GTAGTTGATAATTGATTTGG+TGG	0.461348	5_3:-49223365	Msa0815590:three_prime_UTR
GTAACAATAATCGAAGTTCC+AGG	0.466240	5_3:+49225473	None:intergenic
AAGATCGCTATTTGGAAAGC+TGG	0.469148	5_3:-49225347	Msa0815590:CDS
GGGTTTACGGTTGAAGGTGG+TGG	0.469331	5_3:-49225422	Msa0815590:CDS
CTTTCAATGGGTCGGAATTG+GGG	0.469604	5_3:-49223686	Msa0815590:CDS
AAGGTTAAGGGCTCAAGAAC+AGG	0.472972	5_3:-49223713	Msa0815590:CDS
ATCAGCAATGTTGCTGGCCA+TGG	0.474378	5_3:-49223752	Msa0815590:CDS
GGCGCGTGCGGATCGCCATT+CGG	0.481496	5_3:+49225676	None:intergenic
CTTCCCCTTCTTTCACCGAA+TGG	0.483052	5_3:-49225691	Msa0815590:CDS
ATCTTCTCAAAAGCATCAAT+AGG	0.486912	5_3:+49225365	None:intergenic
ACAATGTTGCTCCCAAAGAC+TGG	0.490580	5_3:-49223577	Msa0815590:CDS
AGAACAGGTTGGCTTTCAAT+GGG	0.491999	5_3:-49223698	Msa0815590:CDS
AGAAGAGTAGTGTGAGGTGA+CGG	0.492028	5_3:+49225745	None:intergenic
TGGAGACATTGTTGCGGTGA+AGG	0.493493	5_3:-49223732	Msa0815590:CDS
TGGATCTACCGGGGCGTAGT+AGG	0.498940	5_3:+49225652	None:intergenic
TTTGAGGTTACGAGTAGTGA+TGG	0.501425	5_3:-49223617	Msa0815590:CDS
CTCGTAACCTCAAATGAAAG+AGG	0.502745	5_3:+49223626	None:intergenic
GATCGCCATTCGGTGAAAGA+AGG	0.504459	5_3:+49225686	None:intergenic
TCAACCGTAAACCCATAATT+CGG	0.504889	5_3:+49225431	None:intergenic
GGGGAAGATTGTTGAGAAGG+TGG	0.507413	5_3:+49225707	None:intergenic
AGAAGGTGGTGGTTGAGAAG+AGG	0.512505	5_3:+49225721	None:intergenic
GAAGGGGAAGATTGTTGAGA+AGG	0.515350	5_3:+49225704	None:intergenic
CTGTGTCACGTGGATCTACC+GGG	0.516254	5_3:+49225642	None:intergenic
ACGAAGAAAACCTGTTTCAA+GGG	0.518898	5_3:-49223504	Msa0815590:three_prime_UTR
TGTTGCAATGGGTGGGACAC+TGG	0.525263	5_3:+49225941	None:intergenic
AGGATAAAGTGCAGAAGGGA+AGG	0.530862	5_3:-49223824	Msa0815590:CDS
TGAAGAAGAAAGAAAAGTGA+GGG	0.531579	5_3:+49225834	None:intergenic
TATGGGTTTACGGTTGAAGG+TGG	0.532788	5_3:-49225425	Msa0815590:CDS
ATCGCCATTCGGTGAAAGAA+GGG	0.538930	5_3:+49225687	None:intergenic
GACACAGTCGGCGGCGCGTG+TGG	0.550703	5_3:-49225626	Msa0815590:CDS
CAGGTTGGCTTTCAATGGGT+CGG	0.553172	5_3:-49223694	Msa0815590:CDS
ATGAAGAAGAAAGAAAAGTG+AGG	0.555383	5_3:+49225833	None:intergenic
TACGGAGACTTATCAAAAGC+CGG	0.565953	5_3:-49225602	Msa0815590:CDS
GGAGCAGGATAAAGTGCAGA+AGG	0.570200	5_3:-49223829	Msa0815590:intron
GGAGTCCGATTTACAGTCAC+CGG	0.573922	5_3:-49223800	Msa0815590:CDS
ATAAAGTGCAGAAGGGAAGG+TGG	0.575254	5_3:-49223821	Msa0815590:CDS
TGAAATGAAGATACCAGATC+CGG	0.576855	5_3:+49223781	None:intergenic
AGAACAAAGTGCTTATTAGG+AGG	0.582144	5_3:+49225389	None:intergenic
TTGTTGGTCAACAACAAAAG+AGG	0.583248	5_3:-49223267	Msa0815590:three_prime_UTR
TTGAGGTTACGAGTAGTGAT+GGG	0.583484	5_3:-49223616	Msa0815590:CDS
TTTGAACGAGGTCAGATCTG+TGG	0.583854	5_3:-49225542	Msa0815590:CDS
AATTAACATGTAAATGCTTG+AGG	0.586547	5_3:-49223244	Msa0815590:three_prime_UTR
AGAGGAAGAAGAGTAGTGTG+AGG	0.594007	5_3:+49225739	None:intergenic
ATCAAAAGCCGGTTACGGCA+TGG	0.595697	5_3:-49225591	Msa0815590:CDS
AATGGGAAAGGGAAAGAGAC+TGG	0.597600	5_3:+49223480	None:intergenic
ACATGCCTCTTCAGTATCGT+AGG	0.598128	5_3:-49225322	Msa0815590:intron
ATCTACCGGGGCGTAGTAGG+TGG	0.607583	5_3:+49225655	None:intergenic
GAAAGTGGTAGTGAAAGTGA+TGG	0.609379	5_3:+49225893	None:intergenic
GGTTACGGCATGGCAACTGT+TGG	0.609475	5_3:-49225581	Msa0815590:CDS
GAAGATTGTTGAGAAGGTGG+TGG	0.611367	5_3:+49225710	None:intergenic
GGCGTAGTAGGTGGCGCGTG+CGG	0.613506	5_3:+49225664	None:intergenic
AAAGTGGTAGTGAAAGTGAT+GGG	0.615535	5_3:+49225894	None:intergenic
GTAACATTTCCTCAGTGTGA+AGG	0.620175	5_3:+49225807	None:intergenic
ATATACCTACGATACTGAAG+AGG	0.621371	5_3:+49225317	None:intergenic
TCACCGCAACAATGTCTCCA+TGG	0.621449	5_3:+49223735	None:intergenic
TTGTAAGATTGCCAATGTGA+TGG	0.622247	5_3:-49223341	Msa0815590:three_prime_UTR
TTAAGGGCTCAAGAACAGGT+TGG	0.623161	5_3:-49223709	Msa0815590:CDS
GCGCCGCCGACTGTGTCACG+TGG	0.625743	5_3:+49225632	None:intergenic
GGTGTGTGGATGATTTAAAG+TGG	0.628924	5_3:-49225502	Msa0815590:CDS
TCGCCATTCGGTGAAAGAAG+GGG	0.643549	5_3:+49225688	None:intergenic
AAAATGTAAGATAACACCAC+CGG	0.645862	5_3:+49223419	None:intergenic
GAGCAGGATAAAGTGCAGAA+GGG	0.649937	5_3:-49223828	Msa0815590:intron
GTGCTTTGAATTACGGTGTG+TGG	0.652130	5_3:-49225516	Msa0815590:CDS
GGAAAGTTACACCATCACAT+TGG	0.657775	5_3:+49223330	None:intergenic
AGTGAAGCTTTGTTTGAACG+AGG	0.664292	5_3:-49225554	Msa0815590:CDS
CTTCATTTACCTTCACACTG+AGG	0.670406	5_3:-49225816	Msa0815590:five_prime_UTR
TAGTGATGGGAAAACAGTCA+CGG	0.670779	5_3:-49223603	Msa0815590:CDS
ACGCGCCACCTACTACGCCC+CGG	0.682530	5_3:-49225660	Msa0815590:CDS
GTAGATCCACGTGACACAGT+CGG	0.684257	5_3:-49225638	Msa0815590:CDS
TAAGTAGACCTATAATATTG+GGG	0.687551	5_3:+49223442	None:intergenic
TGGCCATGGAGACATTGTTG+CGG	0.694232	5_3:-49223738	Msa0815590:CDS
ATTATAGGTCTACTTACCGG+TGG	0.694910	5_3:-49223435	Msa0815590:three_prime_UTR
TGTGTCACGTGGATCTACCG+GGG	0.729868	5_3:+49225643	None:intergenic
GATCCACGTGACACAGTCGG+CGG	0.742025	5_3:-49225635	Msa0815590:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ATCTTACATTTTTATTATTT+TGG	-	chr5_3:49225767-49225786	Msa0815590:CDS	10.0%
!!	AAATCAAAAATTGTATTTGA+GGG	+	chr5_3:49224610-49224629	None:intergenic	15.0%
!!!	TACTTAAAAAAACTTGATTT+TGG	-	chr5_3:49225289-49225308	Msa0815590:intron	15.0%
!!	GGTAAAAAAAAAAATGAAAT+GGG	+	chr5_3:49225715-49225734	None:intergenic	15.0%
!!!	TTATTTTTTGTTGTTGCAAT+GGG	+	chr5_3:49223248-49223267	None:intergenic	20.0%
!!!	TTTATTTTTTGTTGTTGCAA+TGG	+	chr5_3:49223249-49223268	None:intergenic	20.0%
!!	TTTAATAGTATACTTGAGTT+TGG	-	chr5_3:49223986-49224005	Msa0815590:intron	20.0%
!!!	TTGTTAATGGTTAATTTGTT+AGG	-	chr5_3:49224150-49224169	Msa0815590:intron	20.0%
!!!	TGTTAATGGTTAATTTGTTA+GGG	-	chr5_3:49224151-49224170	Msa0815590:intron	20.0%
!!	GAAATCAAAAATTGTATTTG+AGG	+	chr5_3:49224611-49224630	None:intergenic	20.0%
!!	ATACAAAATCCCATTAATTT+GGG	+	chr5_3:49224635-49224654	None:intergenic	20.0%
!!	AATACAAAATCCCATTAATT+TGG	+	chr5_3:49224636-49224655	None:intergenic	20.0%
!!	TACTAATTGAATTCTATGTA+TGG	+	chr5_3:49224911-49224930	None:intergenic	20.0%
!!	ATTAACATTGTATGAAGATA+CGG	+	chr5_3:49224938-49224957	None:intergenic	20.0%
!!!	TTATTAGACCATTAATTTTG+CGG	+	chr5_3:49225111-49225130	None:intergenic	20.0%
!!	AAAAAAAATGAAATGGGAAA+GGG	+	chr5_3:49225709-49225728	None:intergenic	20.0%
!!	AAAAAAAAATGAAATGGGAA+AGG	+	chr5_3:49225710-49225729	None:intergenic	20.0%
!!	GGGTAAAAAAAAAAATGAAA+TGG	+	chr5_3:49225716-49225735	None:intergenic	20.0%
!!!	TTTTTTTACCCCAATATTAT+AGG	-	chr5_3:49225725-49225744	Msa0815590:CDS	20.0%
!!	TATGAAAATATATACCTTGT+TGG	-	chr5_3:49225892-49225911	Msa0815590:five_prime_UTR	20.0%
!!!	GGTTATTTTTATTTTTCCCA+TGG	-	chr5_3:49224023-49224042	Msa0815590:intron	25.0%
!!!	TTTTCAAGAAGATATTCCAT+GGG	+	chr5_3:49224042-49224061	None:intergenic	25.0%
!!!	TTTTTCAAGAAGATATTCCA+TGG	+	chr5_3:49224043-49224062	None:intergenic	25.0%
!	AGTGAGAAACAAATTGTTAA+TGG	-	chr5_3:49224137-49224156	Msa0815590:intron	25.0%
!	AATGAAATTCAAGTAGCAAA+AGG	-	chr5_3:49224351-49224370	Msa0815590:intron	25.0%
!!	AAATTGTATTTGAGGGTTTT+GGG	+	chr5_3:49224603-49224622	None:intergenic	25.0%
!!	AAAATTGTATTTGAGGGTTT+TGG	+	chr5_3:49224604-49224623	None:intergenic	25.0%
!!	TACAAAATCCCATTAATTTG+GGG	+	chr5_3:49224634-49224653	None:intergenic	25.0%
!	AACAAATCCTAAAGGAAAAT+AGG	-	chr5_3:49224771-49224790	Msa0815590:intron	25.0%
!!	ATATTTCGCATTAAGCTTTT+CGG	+	chr5_3:49224819-49224838	None:intergenic	25.0%
!	TCTATGTATGGAAAAATGTT+GGG	+	chr5_3:49224899-49224918	None:intergenic	25.0%
!	TTCTATGTATGGAAAAATGT+TGG	+	chr5_3:49224900-49224919	None:intergenic	25.0%
!	GTATCTTCATACAATGTTAA+TGG	-	chr5_3:49224937-49224956	Msa0815590:intron	25.0%
!	TAAGTAGACCTATAATATTG+GGG	+	chr5_3:49225736-49225755	None:intergenic	25.0%
!	GTAAGTAGACCTATAATATT+GGG	+	chr5_3:49225737-49225756	None:intergenic	25.0%
!	AATATTATAGGTCTACTTAC+CGG	-	chr5_3:49225737-49225756	Msa0815590:CDS	25.0%
!!!	AGAGTAGTTGATAATTGATT+TGG	-	chr5_3:49225807-49225826	Msa0815590:exon	25.0%
!	AACTTTCCATCTATTATCTT+AGG	-	chr5_3:49225860-49225879	Msa0815590:five_prime_UTR	25.0%
!	TAAACTCCTAAGATAATAGA+TGG	+	chr5_3:49225869-49225888	None:intergenic	25.0%
!	AATTAACATGTAAATGCTTG+AGG	-	chr5_3:49225931-49225950	Msa0815590:five_prime_UTR	25.0%
	GAGAAAATGAAGAAAGAAAG+TGG	+	chr5_3:49223300-49223319	None:intergenic	30.0%
	TGAAGAAGAAAGAAAAGTGA+GGG	+	chr5_3:49223344-49223363	None:intergenic	30.0%
	ATGAAGAAGAAAGAAAAGTG+AGG	+	chr5_3:49223345-49223364	None:intergenic	30.0%
!!	ATCTTCTCAAAAGCATCAAT+AGG	+	chr5_3:49223813-49223832	None:intergenic	30.0%
	ACTTGAATTTCATTAGCACT+AGG	+	chr5_3:49224345-49224364	None:intergenic	30.0%
!	AATTGTATTTGAGGGTTTTG+GGG	+	chr5_3:49224602-49224621	None:intergenic	30.0%
!	TTTGATTTCCCCCAAATTAA+TGG	-	chr5_3:49224622-49224641	Msa0815590:intron	30.0%
	TTGATTTCCCCCAAATTAAT+GGG	-	chr5_3:49224623-49224642	Msa0815590:intron	30.0%
!	ACAAAATCCCATTAATTTGG+GGG	+	chr5_3:49224633-49224652	None:intergenic	30.0%
!	CTACTATCCTATTTTCCTTT+AGG	+	chr5_3:49224781-49224800	None:intergenic	30.0%
!	ATATTTTGCTCCATTATGAG+TGG	-	chr5_3:49224835-49224854	Msa0815590:intron	30.0%
	AATTCCTGTAGATAATGAGA+AGG	-	chr5_3:49224976-49224995	Msa0815590:intron	30.0%
	ATTCCTGTAGATAATGAGAA+GGG	-	chr5_3:49224977-49224996	Msa0815590:intron	30.0%
!	ATTTTGCTAGCAAGTACATT+AGG	-	chr5_3:49225017-49225036	Msa0815590:intron	30.0%
!	TTTTGCTAGCAAGTACATTA+GGG	-	chr5_3:49225018-49225037	Msa0815590:intron	30.0%
	AGAAAAGACCGCAAAATTAA+TGG	-	chr5_3:49225100-49225119	Msa0815590:intron	30.0%
	TATGCTACATCATTTACTGT+TGG	-	chr5_3:49225236-49225255	Msa0815590:intron	30.0%
	TTTGAATGTTGTGTTCTGTT+TGG	-	chr5_3:49225325-49225344	Msa0815590:CDS	30.0%
	GGTAAGTAGACCTATAATAT+TGG	+	chr5_3:49225738-49225757	None:intergenic	30.0%
	AAAATGTAAGATAACACCAC+CGG	+	chr5_3:49225759-49225778	None:intergenic	30.0%
!!	GTAGTTGATAATTGATTTGG+TGG	-	chr5_3:49225810-49225829	Msa0815590:exon	30.0%
!!!	TTTTTGTTGTTGCAATGGGT+GGG	+	chr5_3:49223244-49223263	None:intergenic	35.0%
!!!	TTTTTTGTTGTTGCAATGGG+TGG	+	chr5_3:49223245-49223264	None:intergenic	35.0%
!	AAAGTGGTAGTGAAAGTGAT+GGG	+	chr5_3:49223284-49223303	None:intergenic	35.0%
!	GATTTAAAGTGGTGTATTCC+TGG	-	chr5_3:49223684-49223703	Msa0815590:CDS	35.0%
	GTAACAATAATCGAAGTTCC+AGG	+	chr5_3:49223705-49223724	None:intergenic	35.0%
	TCAACCGTAAACCCATAATT+CGG	+	chr5_3:49223747-49223766	None:intergenic	35.0%
	AATTATGGGTTTACGGTTGA+AGG	-	chr5_3:49223747-49223766	Msa0815590:CDS	35.0%
!	AGAACAAAGTGCTTATTAGG+AGG	+	chr5_3:49223789-49223808	None:intergenic	35.0%
!	GGAAGAACAAAGTGCTTATT+AGG	+	chr5_3:49223792-49223811	None:intergenic	35.0%
!!	CTTTTGAGAAGATCGCTATT+TGG	-	chr5_3:49223820-49223839	Msa0815590:CDS	35.0%
	ATATACCTACGATACTGAAG+AGG	+	chr5_3:49223861-49223880	None:intergenic	35.0%
!!	AGTTTGGCTTGTTTATGAGT+TGG	-	chr5_3:49224002-49224021	Msa0815590:intron	35.0%
!!	GTTAATTTGTTAGGGAGAGA+GGG	-	chr5_3:49224159-49224178	Msa0815590:intron	35.0%
	CTTCATGCATAATGCTCATT+TGG	-	chr5_3:49224202-49224221	Msa0815590:intron	35.0%
	AAGAGAAAGGTTTGAATCCT+TGG	+	chr5_3:49224258-49224277	None:intergenic	35.0%
	AGGATTCAAACCTTTCTCTT+TGG	-	chr5_3:49224258-49224277	Msa0815590:intron	35.0%
	GCATAAAGTTCCAAAGAGAA+AGG	+	chr5_3:49224271-49224290	None:intergenic	35.0%
	GAATTTCATTAGCACTAGGA+AGG	+	chr5_3:49224341-49224360	None:intergenic	35.0%
	TCAAGTAGCAAAAGGATGAA+AGG	-	chr5_3:49224359-49224378	Msa0815590:intron	35.0%
	CAAGTAGCAAAAGGATGAAA+GGG	-	chr5_3:49224360-49224379	Msa0815590:intron	35.0%
	TTTGAGGGATGGGAAATATA+GGG	-	chr5_3:49224439-49224458	Msa0815590:intron	35.0%
!!	ATTGTATTTGAGGGTTTTGG+GGG	+	chr5_3:49224601-49224620	None:intergenic	35.0%
	TGGAAGAAATAGTAGGCTTT+GGG	+	chr5_3:49224692-49224711	None:intergenic	35.0%
!	TTAGGATTTGTTTGCGAGTT+TGG	+	chr5_3:49224763-49224782	None:intergenic	35.0%
	AACTCGCAAACAAATCCTAA+AGG	-	chr5_3:49224763-49224782	Msa0815590:intron	35.0%
	CTATTAACCCACGGTTTAAA+TGG	+	chr5_3:49225152-49225171	None:intergenic	35.0%
	ATAGCTGATAAAGGAACTTG+TGG	-	chr5_3:49225178-49225197	Msa0815590:intron	35.0%
	TGAAATGAAGATACCAGATC+CGG	+	chr5_3:49225397-49225416	None:intergenic	35.0%
	GAATCAACCTCTTTCATTTG+AGG	-	chr5_3:49225542-49225561	Msa0815590:CDS	35.0%
!!!	AGTTTTGGACAAACCTTTGA+AGG	-	chr5_3:49225621-49225640	Msa0815590:CDS	35.0%
	ATCAAATTGCTTGCCTTCAA+AGG	+	chr5_3:49225637-49225656	None:intergenic	35.0%
!	TGAAACAGGTTTTCTTCGTT+TGG	+	chr5_3:49225670-49225689	None:intergenic	35.0%
	AACGAAGAAAACCTGTTTCA+AGG	-	chr5_3:49225670-49225689	Msa0815590:CDS	35.0%
	ACGAAGAAAACCTGTTTCAA+GGG	-	chr5_3:49225671-49225690	Msa0815590:CDS	35.0%
	TTGTAAGATTGCCAATGTGA+TGG	-	chr5_3:49225834-49225853	Msa0815590:five_prime_UTR	35.0%
!!!	TCTTTTGTTGTTGACCAACA+AGG	+	chr5_3:49225909-49225928	None:intergenic	35.0%
	TTGTTGGTCAACAACAAAAG+AGG	-	chr5_3:49225908-49225927	Msa0815590:five_prime_UTR	35.0%
!	GAAAGTGGTAGTGAAAGTGA+TGG	+	chr5_3:49223285-49223304	None:intergenic	40.0%
	CTTCATTTACCTTCACACTG+AGG	-	chr5_3:49223359-49223378	Msa0815590:three_prime_UTR	40.0%
	GTAACATTTCCTCAGTGTGA+AGG	+	chr5_3:49223371-49223390	None:intergenic	40.0%
	TACGGAGACTTATCAAAAGC+CGG	-	chr5_3:49223573-49223592	Msa0815590:CDS	40.0%
	GACTTATCAAAAGCCGGTTA+CGG	-	chr5_3:49223579-49223598	Msa0815590:CDS	40.0%
!!	AGTGAAGCTTTGTTTGAACG+AGG	-	chr5_3:49223621-49223640	Msa0815590:CDS	40.0%
	GGTGTGTGGATGATTTAAAG+TGG	-	chr5_3:49223673-49223692	Msa0815590:CDS	40.0%
	AATTTCTGTGCGCCGAATTA+TGG	-	chr5_3:49223732-49223751	Msa0815590:CDS	40.0%
	ATTTCTGTGCGCCGAATTAT+GGG	-	chr5_3:49223733-49223752	Msa0815590:CDS	40.0%
	AAGATCGCTATTTGGAAAGC+TGG	-	chr5_3:49223828-49223847	Msa0815590:intron	40.0%
!	GCTCTAAACGAAAGAGTAGT+TGG	+	chr5_3:49223891-49223910	None:intergenic	40.0%
!!	GGTTAATTTGTTAGGGAGAG+AGG	-	chr5_3:49224158-49224177	Msa0815590:intron	40.0%
	AGAAAGGTTTGAATCCTTGG+AGG	+	chr5_3:49224255-49224274	None:intergenic	40.0%
	AAGGGACTAAATTGACCCTA+AGG	-	chr5_3:49224378-49224397	Msa0815590:intron	40.0%
!!	ATTGACCCTAAGGCTTTGTT+TGG	-	chr5_3:49224388-49224407	Msa0815590:intron	40.0%
!!	TTGACCCTAAGGCTTTGTTT+GGG	-	chr5_3:49224389-49224408	Msa0815590:intron	40.0%
	AACTCCCAAACAAAGCCTTA+GGG	+	chr5_3:49224396-49224415	None:intergenic	40.0%
!!	TAAGGCTTTGTTTGGGAGTT+CGG	-	chr5_3:49224396-49224415	Msa0815590:intron	40.0%
!	CTTTGAGGGATGGGAAATAT+AGG	-	chr5_3:49224438-49224457	Msa0815590:intron	40.0%
	TTGAGGGATGGGAAATATAG+GGG	-	chr5_3:49224440-49224459	Msa0815590:intron	40.0%
	ATGGGAAATATAGGGGGAAA+TGG	-	chr5_3:49224447-49224466	Msa0815590:intron	40.0%
	GTGGAAGAAATAGTAGGCTT+TGG	+	chr5_3:49224693-49224712	None:intergenic	40.0%
	GAGCAAGTGGAAGAAATAGT+AGG	+	chr5_3:49224699-49224718	None:intergenic	40.0%
	ATTTGTTTGCGAGTTTGGAG+GGG	+	chr5_3:49224758-49224777	None:intergenic	40.0%
!	GATTTGTTTGCGAGTTTGGA+GGG	+	chr5_3:49224759-49224778	None:intergenic	40.0%
	ACATTGTATGAAGATACGGC+TGG	+	chr5_3:49224934-49224953	None:intergenic	40.0%
	GTTCCCTTCTCATTATCTAC+AGG	+	chr5_3:49224983-49225002	None:intergenic	40.0%
!	TTTCGTACACGGTTTGAAAC+GGG	+	chr5_3:49225069-49225088	None:intergenic	40.0%
	GTTTCGTACACGGTTTGAAA+CGG	+	chr5_3:49225070-49225089	None:intergenic	40.0%
	TTTCAAACCGTGTACGAAAC+AGG	-	chr5_3:49225070-49225089	Msa0815590:intron	40.0%
	TATGCTTGGCTATTAACCCA+CGG	+	chr5_3:49225161-49225180	None:intergenic	40.0%
	TAGCCAAGCATAGCTGATAA+AGG	-	chr5_3:49225169-49225188	Msa0815590:intron	40.0%
	GTTCCTTTATCAGCTATGCT+TGG	+	chr5_3:49225175-49225194	None:intergenic	40.0%
	TAGCATAATGAGAGCTGTTG+GGG	+	chr5_3:49225223-49225242	None:intergenic	40.0%
	GTAGCATAATGAGAGCTGTT+GGG	+	chr5_3:49225224-49225243	None:intergenic	40.0%
	TGTAGCATAATGAGAGCTGT+TGG	+	chr5_3:49225225-49225244	None:intergenic	40.0%
	ATTGTTGCGGTGAAGGTTAA+GGG	-	chr5_3:49225450-49225469	Msa0815590:CDS	40.0%
!!	AAGAACAGGTTGGCTTTCAA+TGG	-	chr5_3:49225476-49225495	Msa0815590:CDS	40.0%
!!	AGAACAGGTTGGCTTTCAAT+GGG	-	chr5_3:49225477-49225496	Msa0815590:CDS	40.0%
!	ACGCGTTTATATGCCAGTTT+TGG	+	chr5_3:49225515-49225534	None:intergenic	40.0%
	CTCGTAACCTCAAATGAAAG+AGG	+	chr5_3:49225552-49225571	None:intergenic	40.0%
!	TTTGAGGTTACGAGTAGTGA+TGG	-	chr5_3:49225558-49225577	Msa0815590:CDS	40.0%
	TTGAGGTTACGAGTAGTGAT+GGG	-	chr5_3:49225559-49225578	Msa0815590:CDS	40.0%
!	TAGTGATGGGAAAACAGTCA+CGG	-	chr5_3:49225572-49225591	Msa0815590:CDS	40.0%
	TGTCCAAAACTCCAGTCTTT+GGG	+	chr5_3:49225612-49225631	None:intergenic	40.0%
	TTGTCCAAAACTCCAGTCTT+TGG	+	chr5_3:49225613-49225632	None:intergenic	40.0%
	ATTATAGGTCTACTTACCGG+TGG	-	chr5_3:49225740-49225759	Msa0815590:CDS	40.0%
	GGAAAGTTACACCATCACAT+TGG	+	chr5_3:49225848-49225867	None:intergenic	40.0%
	AGAAGAGTAGTGTGAGGTGA+CGG	+	chr5_3:49223433-49223452	None:intergenic	45.0%
	AGAGGAAGAAGAGTAGTGTG+AGG	+	chr5_3:49223439-49223458	None:intergenic	45.0%
	GAAGATTGTTGAGAAGGTGG+TGG	+	chr5_3:49223468-49223487	None:intergenic	45.0%
	GAAGGGGAAGATTGTTGAGA+AGG	+	chr5_3:49223474-49223493	None:intergenic	45.0%
!	ATCGCCATTCGGTGAAAGAA+GGG	+	chr5_3:49223491-49223510	None:intergenic	45.0%
	TTTGAACGAGGTCAGATCTG+TGG	-	chr5_3:49223633-49223652	Msa0815590:CDS	45.0%
!	GTGCTTTGAATTACGGTGTG+TGG	-	chr5_3:49223659-49223678	Msa0815590:CDS	45.0%
	TGCGCCGAATTATGGGTTTA+CGG	-	chr5_3:49223740-49223759	Msa0815590:CDS	45.0%
	TATGGGTTTACGGTTGAAGG+TGG	-	chr5_3:49223750-49223769	Msa0815590:CDS	45.0%
	ACATGCCTCTTCAGTATCGT+AGG	-	chr5_3:49223853-49223872	Msa0815590:intron	45.0%
	GAACTCCCAAACAAAGCCTT+AGG	+	chr5_3:49224397-49224416	None:intergenic	45.0%
	TGAGGGATGGGAAATATAGG+GGG	-	chr5_3:49224441-49224460	Msa0815590:intron	45.0%
	GGAAATATAGGGGGAAATGG+AGG	-	chr5_3:49224450-49224469	Msa0815590:intron	45.0%
!!	GAAATAGTAGGCTTTGGGAG+CGG	+	chr5_3:49224687-49224706	None:intergenic	45.0%
	GGAAAAAAGGAAGGAGCAAG+TGG	+	chr5_3:49224712-49224731	None:intergenic	45.0%
!!	GAGGGCTTTGGAAAAAAGGA+AGG	+	chr5_3:49224721-49224740	None:intergenic	45.0%
!	GGATTTGTTTGCGAGTTTGG+AGG	+	chr5_3:49224760-49224779	None:intergenic	45.0%
	CTCCATTATGAGTGGCAAAC+TGG	-	chr5_3:49224843-49224862	Msa0815590:intron	45.0%
	TGCCAGTTTGCCACTCATAA+TGG	+	chr5_3:49224848-49224867	None:intergenic	45.0%
	GGTTTAAATGGTGCGAACAC+GGG	+	chr5_3:49225140-49225159	None:intergenic	45.0%
	CGGTTTAAATGGTGCGAACA+CGG	+	chr5_3:49225141-49225160	None:intergenic	45.0%
	TGTTCGCACCATTTAAACCG+TGG	-	chr5_3:49225141-49225160	Msa0815590:intron	45.0%
	GTTCGCACCATTTAAACCGT+GGG	-	chr5_3:49225142-49225161	Msa0815590:intron	45.0%
!	TGTTGTGTTCTGTTTGGAGC+AGG	-	chr5_3:49225331-49225350	Msa0815590:CDS	45.0%
	GAGCAGGATAAAGTGCAGAA+GGG	-	chr5_3:49225347-49225366	Msa0815590:CDS	45.0%
	AGGATAAAGTGCAGAAGGGA+AGG	-	chr5_3:49225351-49225370	Msa0815590:CDS	45.0%
	ATAAAGTGCAGAAGGGAAGG+TGG	-	chr5_3:49225354-49225373	Msa0815590:CDS	45.0%
	CAGATCCGGTGACTGTAAAT+CGG	+	chr5_3:49225383-49225402	None:intergenic	45.0%
	CATTGTTGCGGTGAAGGTTA+AGG	-	chr5_3:49225449-49225468	Msa0815590:CDS	45.0%
	AAGGTTAAGGGCTCAAGAAC+AGG	-	chr5_3:49225462-49225481	Msa0815590:CDS	45.0%
	TTAAGGGCTCAAGAACAGGT+TGG	-	chr5_3:49225466-49225485	Msa0815590:CDS	45.0%
	GCTTTCAATGGGTCGGAATT+GGG	-	chr5_3:49225488-49225507	Msa0815590:CDS	45.0%
	CTTTCAATGGGTCGGAATTG+GGG	-	chr5_3:49225489-49225508	Msa0815590:CDS	45.0%
	ACAATGTTGCTCCCAAAGAC+TGG	-	chr5_3:49225598-49225617	Msa0815590:CDS	45.0%
	AATGGGAAAGGGAAAGAGAC+TGG	+	chr5_3:49225698-49225717	None:intergenic	45.0%
!	AGAAGGTGGTGGTTGAGAAG+AGG	+	chr5_3:49223457-49223476	None:intergenic	50.0%
	GGGGAAGATTGTTGAGAAGG+TGG	+	chr5_3:49223471-49223490	None:intergenic	50.0%
	CTTCCCCTTCTTTCACCGAA+TGG	-	chr5_3:49223484-49223503	Msa0815590:three_prime_UTR	50.0%
!	TCGCCATTCGGTGAAAGAAG+GGG	+	chr5_3:49223490-49223509	None:intergenic	50.0%
!	GATCGCCATTCGGTGAAAGA+AGG	+	chr5_3:49223492-49223511	None:intergenic	50.0%
	ACTGTGTCACGTGGATCTAC+CGG	+	chr5_3:49223537-49223556	None:intergenic	50.0%
	GTAGATCCACGTGACACAGT+CGG	-	chr5_3:49223537-49223556	Msa0815590:CDS	50.0%
	ATCAAAAGCCGGTTACGGCA+TGG	-	chr5_3:49223584-49223603	Msa0815590:CDS	50.0%
	AACAGTTGCCATGCCGTAAC+CGG	+	chr5_3:49223595-49223614	None:intergenic	50.0%
!	GTGGCGCGTGCTTTGAATTA+CGG	-	chr5_3:49223652-49223671	Msa0815590:CDS	50.0%
	GGAGGAGCAGTCAAGTGATA+TGG	+	chr5_3:49224237-49224256	None:intergenic	50.0%
	ATCACTTGACTGCTCCTCCA+AGG	-	chr5_3:49224238-49224257	Msa0815590:intron	50.0%
!!	GCTTTGTTTGGGAGTTCGGA+GGG	-	chr5_3:49224400-49224419	Msa0815590:intron	50.0%
!	CTTTGTTTGGGAGTTCGGAG+GGG	-	chr5_3:49224401-49224420	Msa0815590:intron	50.0%
!!	AGGGGAGGGCTTTGGAAAAA+AGG	+	chr5_3:49224725-49224744	None:intergenic	50.0%
	TTTGCGAGTTTGGAGGGGAA+GGG	+	chr5_3:49224753-49224772	None:intergenic	50.0%
	AAACCGTGTACGAAACAGGC+TGG	-	chr5_3:49225074-49225093	Msa0815590:intron	50.0%
	AACCGTGTACGAAACAGGCT+GGG	-	chr5_3:49225075-49225094	Msa0815590:intron	50.0%
	GGAGCAGGATAAAGTGCAGA+AGG	-	chr5_3:49225346-49225365	Msa0815590:CDS	50.0%
	GGAGTCCGATTTACAGTCAC+CGG	-	chr5_3:49225375-49225394	Msa0815590:CDS	50.0%
	CGATTTACAGTCACCGGATC+TGG	-	chr5_3:49225381-49225400	Msa0815590:CDS	50.0%
!	GTGCTGATCAGCAATGTTGC+TGG	-	chr5_3:49225417-49225436	Msa0815590:CDS	50.0%
	ATCAGCAATGTTGCTGGCCA+TGG	-	chr5_3:49225423-49225442	Msa0815590:CDS	50.0%
	TGGCCATGGAGACATTGTTG+CGG	-	chr5_3:49225437-49225456	Msa0815590:CDS	50.0%
	TCACCGCAACAATGTCTCCA+TGG	+	chr5_3:49225443-49225462	None:intergenic	50.0%
	TGGAGACATTGTTGCGGTGA+AGG	-	chr5_3:49225443-49225462	Msa0815590:CDS	50.0%
!!	CAGGTTGGCTTTCAATGGGT+CGG	-	chr5_3:49225481-49225500	Msa0815590:CDS	50.0%
	GGCTTTCAATGGGTCGGAAT+TGG	-	chr5_3:49225487-49225506	Msa0815590:CDS	50.0%
!	GCTCCCAAAGACTGGAGTTT+TGG	-	chr5_3:49225606-49225625	Msa0815590:CDS	50.0%
!	AGAGACTGGTCCCTTGAAAC+AGG	+	chr5_3:49225684-49225703	None:intergenic	50.0%
	TGTTGCAATGGGTGGGACAC+TGG	+	chr5_3:49223237-49223256	None:intergenic	55.0%
	TGTGTCACGTGGATCTACCG+GGG	+	chr5_3:49223535-49223554	None:intergenic	55.0%
	CTGTGTCACGTGGATCTACC+GGG	+	chr5_3:49223536-49223555	None:intergenic	55.0%
	GGTTACGGCATGGCAACTGT+TGG	-	chr5_3:49223594-49223613	Msa0815590:CDS	55.0%
	GGGTTTACGGTTGAAGGTGG+TGG	-	chr5_3:49223753-49223772	Msa0815590:CDS	55.0%
!!	GGCTTTGTTTGGGAGTTCGG+AGG	-	chr5_3:49224399-49224418	Msa0815590:intron	55.0%
!!	TAGTAGGCTTTGGGAGCGGA+GGG	+	chr5_3:49224683-49224702	None:intergenic	55.0%
!!	ATAGTAGGCTTTGGGAGCGG+AGG	+	chr5_3:49224684-49224703	None:intergenic	55.0%
	GTTTGCGAGTTTGGAGGGGA+AGG	+	chr5_3:49224754-49224773	None:intergenic	55.0%
	AGCCCAGCCTGTTTCGTACA+CGG	+	chr5_3:49225080-49225099	None:intergenic	55.0%
	GTCGGAATTGGGGCCAAAAC+TGG	-	chr5_3:49225499-49225518	Msa0815590:CDS	55.0%
	ATCTACCGGGGCGTAGTAGG+TGG	+	chr5_3:49223523-49223542	None:intergenic	60.0%
	TGGATCTACCGGGGCGTAGT+AGG	+	chr5_3:49223526-49223545	None:intergenic	60.0%
	GATCCACGTGACACAGTCGG+CGG	-	chr5_3:49223540-49223559	Msa0815590:CDS	60.0%
	TGTTTGGGAGTTCGGAGGGG+AGG	-	chr5_3:49224404-49224423	Msa0815590:intron	60.0%
	GTTTGGGAGTTCGGAGGGGA+GGG	-	chr5_3:49224405-49224424	Msa0815590:intron	60.0%
	TTTGGGAGTTCGGAGGGGAG+GGG	-	chr5_3:49224406-49224425	Msa0815590:intron	60.0%
!!	AGGGGAGGGCTTTGAGGGAT+GGG	-	chr5_3:49224429-49224448	Msa0815590:intron	60.0%
	TTTGGGAGCGGAGGGCTTTG+GGG	+	chr5_3:49224675-49224694	None:intergenic	60.0%
!	CTTTGGGAGCGGAGGGCTTT+GGG	+	chr5_3:49224676-49224695	None:intergenic	60.0%
	GAGTTTGGAGGGGAAGGGAG+GGG	+	chr5_3:49224748-49224767	None:intergenic	60.0%
	CGAGTTTGGAGGGGAAGGGA+GGG	+	chr5_3:49224749-49224768	None:intergenic	60.0%
!	AGGGGAGGGGAGGGCTTTGA+GGG	-	chr5_3:49224424-49224443	Msa0815590:intron	65.0%
!!	GAGGGGAGGGCTTTGAGGGA+TGG	-	chr5_3:49224428-49224447	Msa0815590:intron	65.0%
!	TTGGGAGCGGAGGGCTTTGG+GGG	+	chr5_3:49224674-49224693	None:intergenic	65.0%
!!	GCTTTGGGAGCGGAGGGCTT+TGG	+	chr5_3:49224677-49224696	None:intergenic	65.0%
	TTGGAGGGGAAGGGAGGGGA+GGG	+	chr5_3:49224744-49224763	None:intergenic	65.0%
	TTTGGAGGGGAAGGGAGGGG+AGG	+	chr5_3:49224745-49224764	None:intergenic	65.0%
	GCGAGTTTGGAGGGGAAGGG+AGG	+	chr5_3:49224750-49224769	None:intergenic	65.0%
	GGCGCGTGCGGATCGCCATT+CGG	+	chr5_3:49223502-49223521	None:intergenic	70.0%
	GGCGTAGTAGGTGGCGCGTG+CGG	+	chr5_3:49223514-49223533	None:intergenic	70.0%
	ACGCGCCACCTACTACGCCC+CGG	-	chr5_3:49223515-49223534	Msa0815590:three_prime_UTR	70.0%
	GGAGTTCGGAGGGGAGGGGA+GGG	-	chr5_3:49224410-49224429	Msa0815590:intron	70.0%
	GAGTTCGGAGGGGAGGGGAG+GGG	-	chr5_3:49224411-49224430	Msa0815590:intron	70.0%
	GAGGGGAGGGGAGGGCTTTG+AGG	-	chr5_3:49224423-49224442	Msa0815590:intron	70.0%
	TGGAGGGGAAGGGAGGGGAG+GGG	+	chr5_3:49224743-49224762	None:intergenic	70.0%
!	GCGCCGCCGACTGTGTCACG+TGG	+	chr5_3:49223546-49223565	None:intergenic	75.0%
	GACACAGTCGGCGGCGCGTG+TGG	-	chr5_3:49223549-49223568	Msa0815590:CDS	75.0%
	GTCGGCGGCGCGTGTGGCTA+CGG	-	chr5_3:49223555-49223574	Msa0815590:CDS	75.0%
	GGGAGTTCGGAGGGGAGGGG+AGG	-	chr5_3:49224409-49224428	Msa0815590:intron	75.0%
	TTCGGAGGGGAGGGGAGGGG+AGG	-	chr5_3:49224414-49224433	Msa0815590:intron	75.0%
	TCGGAGGGGAGGGGAGGGGA+GGG	-	chr5_3:49224415-49224434	Msa0815590:intron	75.0%
	GGGAGGGGAGGGGAGGGCTT+TGG	+	chr5_3:49224421-49224440	None:intergenic	75.0%
	GGGGAAGGGAGGGGAGGGGA+GGG	+	chr5_3:49224739-49224758	None:intergenic	75.0%
	AGGGGAAGGGAGGGGAGGGG+AGG	+	chr5_3:49224740-49224759	None:intergenic	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr5_3	gene	49223219	49225978	49223219	ID=Msa0815590;Name=Msa0815590
chr5_3	mRNA	49223219	49225978	49223219	ID=Msa0815590-mRNA-1;Parent=Msa0815590;Name=Msa0815590-mRNA-1;_AED=0.04;_eAED=0.04;_QI=165\|1\|1\|1\|1\|1\|2\|316\|266
chr5_3	exon	49223219	49223844	49223219	ID=Msa0815590-mRNA-1:exon:14799;Parent=Msa0815590-mRNA-1
chr5_3	exon	49225323	49225978	49225323	ID=Msa0815590-mRNA-1:exon:14798;Parent=Msa0815590-mRNA-1
chr5_3	five_prime_UTR	49225814	49225978	49225814	ID=Msa0815590-mRNA-1:five_prime_utr;Parent=Msa0815590-mRNA-1
chr5_3	CDS	49225323	49225813	49225323	ID=Msa0815590-mRNA-1:cds;Parent=Msa0815590-mRNA-1
chr5_3	CDS	49223535	49223844	49223535	ID=Msa0815590-mRNA-1:cds;Parent=Msa0815590-mRNA-1
chr5_3	three_prime_UTR	49223219	49223534	49223219	ID=Msa0815590-mRNA-1:three_prime_utr;Parent=Msa0815590-mRNA-1

Gene Sequence

>Msa0815590

ATGTTACTTACAACGTTCTTCTTTCTCTTTACACTCTCATCAACAACCGTCACCTCACACTACTCTTCTTCCTCTTCTCAACCACCACCTTCTCAACAATCTTCCCCTTCTTTCACCGAATGGCGATCCGCACGCGCCACCTACTACGCCCCGGTAGATCCACGTGACACAGTCGGCGGCGCGTGTGGCTACGGAGACTTATCAAAAGCCGGTTACGGCATGGCAACTGTTGGTCTTAGTGAAGCTTTGTTTGAACGAGGTCAGATCTGTGGCGCGTGCTTTGAATTACGGTGTGTGGATGATTTAAAGTGGTGTATTCCTGGAACTTCGATTATTGTTACTGCGACAAATTTCTGTGCGCCGAATTATGGGTTTACGGTTGAAGGTGGTGGACATTGTAATCCTCCTAATAAGCACTTTGTTCTTCCTATTGATGCTTTTGAGAAGATCGCTATTTGGAAAGCTGGTAACATGCCTCTTCAGTATCGTAGGATAAAGTGCAGAAGGGAAGGTGGAGTCCGATTTACAGTCACCGGATCTGGTATCTTCATTTCAGTGCTGATCAGCAATGTTGCTGGCCATGGAGACATTGTTGCGGTGAAGGTTAAGGGCTCAAGAACAGGTTGGCTTTCAATGGGTCGGAATTGGGGCCAAAACTGGCATATAAACGCGTTACTACAGAATCAACCTCTTTCATTTGAGGTTACGAGTAGTGATGGGAAAACAGTCACGGCTTACAATGTTGCTCCCAAAGACTGGAGTTTTGGACAAACCTTTGAAGGCAAGCAATTTGATTATTAA

Protein sequence

>Msa0815590

MLLTTFFFLFTLSSTTVTSHYSSSSSQPPPSQQSSPSFTEWRSARATYYAPVDPRDTVGGACGYGDLSKAGYGMATVGLSEALFERGQICGACFELRCVDDLKWCIPGTSIIVTATNFCAPNYGFTVEGGGHCNPPNKHFVLPIDAFEKIAIWKAGNMPLQYRRIKCRREGGVRFTVTGSGIFISVLISNVAGHGDIVAVKVKGSRTGWLSMGRNWGQNWHINALLQNQPLSFEVTSSDGKTVTAYNVAPKDWSFGQTFEGKQFDY*