AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0827930

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0827930 MtrunA17_Chr5g0438671 99.408 169 1 0 1 169 584 752 4.71e-113 337
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0827930 AT2G32900.1 65.244 164 52 1 1 164 579 737 1.79e-67 218

Find 55 sgRNAs with CRISPR-Local

Find 223 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCCGCTACCTCAAATATT+TGG 0.180434 5_3:-69894202 None:intergenic
ATGCACTTCTTGTAAGTTAA+AGG 0.187549 5_3:-69893265 None:intergenic
GGTAGCGGAAAGTTAGTTTA+TGG 0.194085 5_3:+69894214 Msa0827930:three_prime_UTR
CTACTAATGGCAAGCATATT+AGG 0.209103 5_3:+69894414 Msa0827930:three_prime_UTR
GCAGGGAACTCACTCAAATT+TGG 0.242142 5_3:-69893531 None:intergenic
AACATTTGTAGCTCAAATTT+CGG 0.273455 5_3:+69894487 Msa0827930:three_prime_UTR
CGTAAAATCCGCAAACTATC+AGG 0.292468 5_3:+69893585 Msa0827930:CDS
TACAGGATCTTGGGAGAATA+AGG 0.315580 5_3:+69893737 Msa0827930:CDS
TAAAATCCATTACAGGATCT+TGG 0.317258 5_3:+69893727 Msa0827930:CDS
GCAAACATGGGGATGGGATT+GGG 0.317373 5_3:+69894350 Msa0827930:three_prime_UTR
ATCATCTTTATTTGAGTCCT+TGG 0.342033 5_3:+69893497 Msa0827930:CDS
ATGCCTTTAAAATCCATTAC+AGG 0.361134 5_3:+69893720 Msa0827930:CDS
CTTTAAGAAAAGATTGTTTA+CGG 0.378098 5_3:+69894043 Msa0827930:CDS
AGAAATGAGAAAGGATTAAG+AGG 0.388297 5_3:+69894580 Msa0827930:three_prime_UTR
CAGTTGTGGCTTTACTATAT+CGG 0.395606 5_3:+69893767 Msa0827930:CDS
AAAATCCATTACAGGATCTT+GGG 0.398335 5_3:+69893728 Msa0827930:CDS
GCTAATTTGTCATCTACTAA+TGG 0.407966 5_3:+69894401 Msa0827930:three_prime_UTR
AGCAAACATGGGGATGGGAT+TGG 0.428825 5_3:+69894349 Msa0827930:three_prime_UTR
GGTATAATAAATTCAACAGT+TGG 0.435917 5_3:+69894601 Msa0827930:three_prime_UTR
GGCTAAATTCAGCATTGATC+AGG 0.438119 5_3:+69892980 Msa0827930:CDS
TGATCAGGTTGTCTTCAGTC+TGG 0.443164 5_3:+69893207 Msa0827930:intron
GCTAGTTCTCCAAATATTTG+AGG 0.444289 5_3:+69894193 Msa0827930:three_prime_UTR
TTATGTATCATGTAGGAAGT+TGG 0.445756 5_3:-69894097 None:intergenic
TTATTTGAGTCCTTGGTCAC+TGG 0.450943 5_3:+69893504 Msa0827930:CDS
AGGACTCAAATAAAGATGAT+AGG 0.451013 5_3:-69893494 None:intergenic
AGCTGCAGAAGAGACTTTAC+AGG 0.452027 5_3:+69893351 Msa0827930:CDS
CTTCAGAAGAGAAATGAGAA+AGG 0.452315 5_3:+69894571 Msa0827930:three_prime_UTR
AAAACATGCCTGATAGTTTG+CGG 0.481396 5_3:-69893593 None:intergenic
TCTCCAAATATTTGAGGTAG+CGG 0.506381 5_3:+69894199 Msa0827930:three_prime_UTR
AGATCTTCAAGAGACTCTGC+AGG 0.510347 5_3:-69893549 None:intergenic
ATTCTCCCAAGATCCTGTAA+TGG 0.510661 5_3:-69893733 None:intergenic
CAAACATGGGGATGGGATTG+GGG 0.515682 5_3:+69894351 Msa0827930:three_prime_UTR
AAGACTAATCCATTTAATGC+TGG 0.519225 5_3:+69893467 Msa0827930:CDS
TAAGTTAAAGGAAGCAAAAG+TGG 0.523217 5_3:-69893253 None:intergenic
TTAAGATTTATGTATCATGT+AGG 0.536340 5_3:-69894104 None:intergenic
TTTACTGTTTAGAGCAAACA+TGG 0.546522 5_3:+69894337 Msa0827930:three_prime_UTR
TAGGCTATAGATAGTGCTGA+TGG 0.550569 5_3:+69892921 None:intergenic
AATAAGGAGTTGATCAGTTG+TGG 0.563312 5_3:+69893753 Msa0827930:CDS
GTTTAGAGCAAACATGGGGA+TGG 0.563622 5_3:+69894343 Msa0827930:three_prime_UTR
AATCTTAAAACACTCGAGGT+GGG 0.563723 5_3:+69894119 Msa0827930:three_prime_UTR
TTTAGAGCAAACATGGGGAT+GGG 0.564659 5_3:+69894344 Msa0827930:three_prime_UTR
ATGTTTGCTCTAAACAGTAA+AGG 0.565232 5_3:-69894335 None:intergenic
ATCTTAAAACACTCGAGGTG+GGG 0.571831 5_3:+69894120 Msa0827930:three_prime_UTR
AAATCTTAAAACACTCGAGG+TGG 0.580083 5_3:+69894118 Msa0827930:three_prime_UTR
TACTGTTTAGAGCAAACATG+GGG 0.625569 5_3:+69894339 Msa0827930:three_prime_UTR
ATTTGGATCACCAGTGACCA+AGG 0.632851 5_3:-69893514 None:intergenic
TTGTGGCTTTACTATATCGG+AGG 0.633013 5_3:+69893770 Msa0827930:CDS
TAAGAAAAGATTGTTTACGG+AGG 0.635266 5_3:+69894046 Msa0827930:CDS
TTACTGTTTAGAGCAAACAT+GGG 0.647291 5_3:+69894338 Msa0827930:three_prime_UTR
CATAAATCTTAAAACACTCG+AGG 0.658578 5_3:+69894115 Msa0827930:three_prime_UTR
TCAGATGCAAGAATTTGAGT+CGG 0.666155 5_3:+69892959 Msa0827930:exon
GATCTTCAAGAGACTCTGCA+GGG 0.673164 5_3:-69893548 None:intergenic
ACGACAACAAAATCGTCATG+AGG 0.675305 5_3:-69894275 None:intergenic
ATATATTATGCCTACCAGTG+AGG 0.705207 5_3:+69894440 Msa0827930:three_prime_UTR
ATTAGATGATGATCCCTCCG+AGG 0.726938 5_3:+69894151 Msa0827930:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTTGTTACAACTTACAAT+TGG + chr5_3:69893122-69893141 Msa0827930:intron 20.0%
!! TATACGAGTAAATAATAATC+CGG - chr5_3:69893653-69893672 None:intergenic 20.0%
!!! TAACATTTTATCAGAATCAT+TGG - chr5_3:69893884-69893903 None:intergenic 20.0%
!!! CTTTAAGAAAAGATTGTTTA+CGG + chr5_3:69894043-69894062 Msa0827930:CDS 20.0%
!! TTAAGATTTATGTATCATGT+AGG - chr5_3:69894107-69894126 None:intergenic 20.0%
!!! CTACAAATGTTTTATATATC+TGG - chr5_3:69894478-69894497 None:intergenic 20.0%
!!! AATTTGTTACAACTTACAAT+TGG + chr5_3:69893122-69893141 Msa0827930:intron 20.0%
!! TATACGAGTAAATAATAATC+CGG - chr5_3:69893653-69893672 None:intergenic 20.0%
!!! TAACATTTTATCAGAATCAT+TGG - chr5_3:69893884-69893903 None:intergenic 20.0%
!!! CTTTAAGAAAAGATTGTTTA+CGG + chr5_3:69894043-69894062 Msa0827930:CDS 20.0%
!! TTAAGATTTATGTATCATGT+AGG - chr5_3:69894107-69894126 None:intergenic 20.0%
!!! CTACAAATGTTTTATATATC+TGG - chr5_3:69894478-69894497 None:intergenic 20.0%
! AAGCTAAAATTTACTTGATC+AGG + chr5_3:69893192-69893211 Msa0827930:intron 25.0%
! ACAGTGTCTACATAAAAATT+GGG - chr5_3:69893627-69893646 None:intergenic 25.0%
! AACAGTGTCTACATAAAAAT+TGG - chr5_3:69893628-69893647 None:intergenic 25.0%
!! AATTTTTATGTAGACACTGT+TGG + chr5_3:69893627-69893646 Msa0827930:intron 25.0%
!!! TCAATGGCTTTATTTCTTTA+TGG + chr5_3:69893806-69893825 Msa0827930:intron 25.0%
! AAGAAAAGGTTCATTAAGTA+CGG - chr5_3:69893930-69893949 None:intergenic 25.0%
! ATATGTAAGAAACATATGTG+AGG - chr5_3:69893965-69893984 None:intergenic 25.0%
!!! TTTTTTTTTGATCTCATTGC+AGG + chr5_3:69893984-69894003 Msa0827930:intron 25.0%
!! CGTAAACAATCTTTTCTTAA+AGG - chr5_3:69894045-69894064 None:intergenic 25.0%
! AACATTTGTAGCTCAAATTT+CGG + chr5_3:69894487-69894506 Msa0827930:three_prime_UTR 25.0%
!!! ACACTTTTTTTATCGAGTTA+AGG + chr5_3:69894515-69894534 Msa0827930:three_prime_UTR 25.0%
! GGTATAATAAATTCAACAGT+TGG + chr5_3:69894601-69894620 Msa0827930:three_prime_UTR 25.0%
! AAGCTAAAATTTACTTGATC+AGG + chr5_3:69893192-69893211 Msa0827930:intron 25.0%
! ACAGTGTCTACATAAAAATT+GGG - chr5_3:69893627-69893646 None:intergenic 25.0%
! AACAGTGTCTACATAAAAAT+TGG - chr5_3:69893628-69893647 None:intergenic 25.0%
!! AATTTTTATGTAGACACTGT+TGG + chr5_3:69893627-69893646 Msa0827930:intron 25.0%
!!! TCAATGGCTTTATTTCTTTA+TGG + chr5_3:69893806-69893825 Msa0827930:intron 25.0%
! AAGAAAAGGTTCATTAAGTA+CGG - chr5_3:69893930-69893949 None:intergenic 25.0%
! ATATGTAAGAAACATATGTG+AGG - chr5_3:69893965-69893984 None:intergenic 25.0%
!!! TTTTTTTTTGATCTCATTGC+AGG + chr5_3:69893984-69894003 Msa0827930:intron 25.0%
!! CGTAAACAATCTTTTCTTAA+AGG - chr5_3:69894045-69894064 None:intergenic 25.0%
! AACATTTGTAGCTCAAATTT+CGG + chr5_3:69894487-69894506 Msa0827930:three_prime_UTR 25.0%
!!! ACACTTTTTTTATCGAGTTA+AGG + chr5_3:69894515-69894534 Msa0827930:three_prime_UTR 25.0%
! GGTATAATAAATTCAACAGT+TGG + chr5_3:69894601-69894620 Msa0827930:three_prime_UTR 25.0%
TAGGAGATAAAGCAAACTAT+GGG - chr5_3:69893057-69893076 None:intergenic 30.0%
ATAGGAGATAAAGCAAACTA+TGG - chr5_3:69893058-69893077 None:intergenic 30.0%
GAAGAAAAAAAGGAGGAAAT+AGG - chr5_3:69893076-69893095 None:intergenic 30.0%
ATTCAGAGAAGAAAAAAAGG+AGG - chr5_3:69893083-69893102 None:intergenic 30.0%
TGCATTCAGAGAAGAAAAAA+AGG - chr5_3:69893086-69893105 None:intergenic 30.0%
TACAATTGGCTAACTAAGAA+TGG + chr5_3:69893136-69893155 Msa0827930:intron 30.0%
TGTCATTCGACAAACTAAAA+GGG - chr5_3:69893171-69893190 None:intergenic 30.0%
ATGTCATTCGACAAACTAAA+AGG - chr5_3:69893172-69893191 None:intergenic 30.0%
! TGGAAAAAGTGCATATCATA+TGG + chr5_3:69893227-69893246 Msa0827930:CDS 30.0%
! GGAAAAAGTGCATATCATAT+GGG + chr5_3:69893228-69893247 Msa0827930:CDS 30.0%
TAAGTTAAAGGAAGCAAAAG+TGG - chr5_3:69893256-69893275 None:intergenic 30.0%
ATGCACTTCTTGTAAGTTAA+AGG - chr5_3:69893268-69893287 None:intergenic 30.0%
! TATTAGAGTCAGTTTTCTCT+AGG + chr5_3:69893296-69893315 Msa0827930:CDS 30.0%
GTGGAAAGTCTTAACTAAAA+TGG - chr5_3:69893417-69893436 None:intergenic 30.0%
AAGACTAATCCATTTAATGC+TGG + chr5_3:69893467-69893486 Msa0827930:CDS 30.0%
! ATAGGTTTTCCAGCATTAAA+TGG - chr5_3:69893479-69893498 None:intergenic 30.0%
AGGACTCAAATAAAGATGAT+AGG - chr5_3:69893497-69893516 None:intergenic 30.0%
! ATCATCTTTATTTGAGTCCT+TGG + chr5_3:69893497-69893516 Msa0827930:CDS 30.0%
!! ATGCCTTTAAAATCCATTAC+AGG + chr5_3:69893720-69893739 Msa0827930:CDS 30.0%
!!! GATCCTGTAATGGATTTTAA+AGG - chr5_3:69893726-69893745 None:intergenic 30.0%
TAAAATCCATTACAGGATCT+TGG + chr5_3:69893727-69893746 Msa0827930:CDS 30.0%
AAAATCCATTACAGGATCTT+GGG + chr5_3:69893728-69893747 Msa0827930:CDS 30.0%
AGGTATCATTTGGTTATCAA+TGG + chr5_3:69893790-69893809 Msa0827930:intron 30.0%
!! TCTTTATGGCTTTATAACTG+AGG + chr5_3:69893820-69893839 Msa0827930:intron 30.0%
!! CTTTATGGCTTTATAACTGA+GGG + chr5_3:69893821-69893840 Msa0827930:intron 30.0%
!! TATCAGAATCATTGGTAATG+TGG - chr5_3:69893876-69893895 None:intergenic 30.0%
TAAGAAAAGATTGTTTACGG+AGG + chr5_3:69894046-69894065 Msa0827930:CDS 30.0%
! GATACAAAATACGAGCTTTT+AGG + chr5_3:69894068-69894087 Msa0827930:CDS 30.0%
TTATGTATCATGTAGGAAGT+TGG - chr5_3:69894100-69894119 None:intergenic 30.0%
CATAAATCTTAAAACACTCG+AGG + chr5_3:69894115-69894134 Msa0827930:three_prime_UTR 30.0%
!! AAACTAACATGTTTTTGCCT+CGG - chr5_3:69894171-69894190 None:intergenic 30.0%
! ATGCTGATAATTTTCTGACA+TGG + chr5_3:69894246-69894265 Msa0827930:three_prime_UTR 30.0%
! ATGTTTGCTCTAAACAGTAA+AGG - chr5_3:69894338-69894357 None:intergenic 30.0%
! TTTACTGTTTAGAGCAAACA+TGG + chr5_3:69894337-69894356 Msa0827930:three_prime_UTR 30.0%
! TTACTGTTTAGAGCAAACAT+GGG + chr5_3:69894338-69894357 Msa0827930:three_prime_UTR 30.0%
! GCTAATTTGTCATCTACTAA+TGG + chr5_3:69894401-69894420 Msa0827930:three_prime_UTR 30.0%
AGAAATGAGAAAGGATTAAG+AGG + chr5_3:69894580-69894599 Msa0827930:three_prime_UTR 30.0%
! ATTCAACAGTTGGTTTCTAA+AGG + chr5_3:69894611-69894630 Msa0827930:three_prime_UTR 30.0%
TAGGAGATAAAGCAAACTAT+GGG - chr5_3:69893057-69893076 None:intergenic 30.0%
ATAGGAGATAAAGCAAACTA+TGG - chr5_3:69893058-69893077 None:intergenic 30.0%
GAAGAAAAAAAGGAGGAAAT+AGG - chr5_3:69893076-69893095 None:intergenic 30.0%
ATTCAGAGAAGAAAAAAAGG+AGG - chr5_3:69893083-69893102 None:intergenic 30.0%
TGCATTCAGAGAAGAAAAAA+AGG - chr5_3:69893086-69893105 None:intergenic 30.0%
TACAATTGGCTAACTAAGAA+TGG + chr5_3:69893136-69893155 Msa0827930:intron 30.0%
TGTCATTCGACAAACTAAAA+GGG - chr5_3:69893171-69893190 None:intergenic 30.0%
ATGTCATTCGACAAACTAAA+AGG - chr5_3:69893172-69893191 None:intergenic 30.0%
! TGGAAAAAGTGCATATCATA+TGG + chr5_3:69893227-69893246 Msa0827930:CDS 30.0%
! GGAAAAAGTGCATATCATAT+GGG + chr5_3:69893228-69893247 Msa0827930:CDS 30.0%
TAAGTTAAAGGAAGCAAAAG+TGG - chr5_3:69893256-69893275 None:intergenic 30.0%
ATGCACTTCTTGTAAGTTAA+AGG - chr5_3:69893268-69893287 None:intergenic 30.0%
! TATTAGAGTCAGTTTTCTCT+AGG + chr5_3:69893296-69893315 Msa0827930:CDS 30.0%
GTGGAAAGTCTTAACTAAAA+TGG - chr5_3:69893417-69893436 None:intergenic 30.0%
AAGACTAATCCATTTAATGC+TGG + chr5_3:69893467-69893486 Msa0827930:CDS 30.0%
! ATAGGTTTTCCAGCATTAAA+TGG - chr5_3:69893479-69893498 None:intergenic 30.0%
AGGACTCAAATAAAGATGAT+AGG - chr5_3:69893497-69893516 None:intergenic 30.0%
! ATCATCTTTATTTGAGTCCT+TGG + chr5_3:69893497-69893516 Msa0827930:CDS 30.0%
!! ATGCCTTTAAAATCCATTAC+AGG + chr5_3:69893720-69893739 Msa0827930:CDS 30.0%
!!! GATCCTGTAATGGATTTTAA+AGG - chr5_3:69893726-69893745 None:intergenic 30.0%
TAAAATCCATTACAGGATCT+TGG + chr5_3:69893727-69893746 Msa0827930:CDS 30.0%
AAAATCCATTACAGGATCTT+GGG + chr5_3:69893728-69893747 Msa0827930:CDS 30.0%
AGGTATCATTTGGTTATCAA+TGG + chr5_3:69893790-69893809 Msa0827930:intron 30.0%
!! TCTTTATGGCTTTATAACTG+AGG + chr5_3:69893820-69893839 Msa0827930:intron 30.0%
!! CTTTATGGCTTTATAACTGA+GGG + chr5_3:69893821-69893840 Msa0827930:intron 30.0%
!! TATCAGAATCATTGGTAATG+TGG - chr5_3:69893876-69893895 None:intergenic 30.0%
TAAGAAAAGATTGTTTACGG+AGG + chr5_3:69894046-69894065 Msa0827930:CDS 30.0%
! GATACAAAATACGAGCTTTT+AGG + chr5_3:69894068-69894087 Msa0827930:CDS 30.0%
TTATGTATCATGTAGGAAGT+TGG - chr5_3:69894100-69894119 None:intergenic 30.0%
CATAAATCTTAAAACACTCG+AGG + chr5_3:69894115-69894134 Msa0827930:three_prime_UTR 30.0%
!! AAACTAACATGTTTTTGCCT+CGG - chr5_3:69894171-69894190 None:intergenic 30.0%
! ATGCTGATAATTTTCTGACA+TGG + chr5_3:69894246-69894265 Msa0827930:three_prime_UTR 30.0%
! ATGTTTGCTCTAAACAGTAA+AGG - chr5_3:69894338-69894357 None:intergenic 30.0%
! TTTACTGTTTAGAGCAAACA+TGG + chr5_3:69894337-69894356 Msa0827930:three_prime_UTR 30.0%
! TTACTGTTTAGAGCAAACAT+GGG + chr5_3:69894338-69894357 Msa0827930:three_prime_UTR 30.0%
! GCTAATTTGTCATCTACTAA+TGG + chr5_3:69894401-69894420 Msa0827930:three_prime_UTR 30.0%
AGAAATGAGAAAGGATTAAG+AGG + chr5_3:69894580-69894599 Msa0827930:three_prime_UTR 30.0%
TCAGATGCAAGAATTTGAGT+CGG + chr5_3:69892959-69892978 Msa0827930:exon 35.0%
TCTGAATGCAAGATACAATG+TGG + chr5_3:69893096-69893115 Msa0827930:intron 35.0%
CTGAATGCAAGATACAATGT+GGG + chr5_3:69893097-69893116 Msa0827930:intron 35.0%
TGAATGCAAGATACAATGTG+GGG + chr5_3:69893098-69893117 Msa0827930:intron 35.0%
ATAAGAGTGGAATGCATTAG+TGG - chr5_3:69893436-69893455 None:intergenic 35.0%
TTTGTAGCTGGAAATAAGAG+TGG - chr5_3:69893449-69893468 None:intergenic 35.0%
!! AATGGATTAGTCTTTGTAGC+TGG - chr5_3:69893461-69893480 None:intergenic 35.0%
! TGCGGATTTTACGTAAAGAT+GGG - chr5_3:69893578-69893597 None:intergenic 35.0%
! TTGCGGATTTTACGTAAAGA+TGG - chr5_3:69893579-69893598 None:intergenic 35.0%
AAAACATGCCTGATAGTTTG+CGG - chr5_3:69893596-69893615 None:intergenic 35.0%
TCTAATAATTCTGCAGCAGA+AGG - chr5_3:69893699-69893718 None:intergenic 35.0%
! AATAAGGAGTTGATCAGTTG+TGG + chr5_3:69893753-69893772 Msa0827930:CDS 35.0%
! CAGTTGTGGCTTTACTATAT+CGG + chr5_3:69893767-69893786 Msa0827930:CDS 35.0%
!! ACTATATCGGAGGTATCATT+TGG + chr5_3:69893780-69893799 Msa0827930:intron 35.0%
!!! TTTTTTGATCTCATTGCAGG+TGG + chr5_3:69893987-69894006 Msa0827930:intron 35.0%
AAATCTTAAAACACTCGAGG+TGG + chr5_3:69894118-69894137 Msa0827930:three_prime_UTR 35.0%
AATCTTAAAACACTCGAGGT+GGG + chr5_3:69894119-69894138 Msa0827930:three_prime_UTR 35.0%
! GCTAGTTCTCCAAATATTTG+AGG + chr5_3:69894193-69894212 Msa0827930:three_prime_UTR 35.0%
! TCTCCAAATATTTGAGGTAG+CGG + chr5_3:69894199-69894218 Msa0827930:three_prime_UTR 35.0%
TTTCCGCTACCTCAAATATT+TGG - chr5_3:69894205-69894224 None:intergenic 35.0%
! CTGATAATTTTCTGACATGG+AGG + chr5_3:69894249-69894268 Msa0827930:three_prime_UTR 35.0%
! TACTGTTTAGAGCAAACATG+GGG + chr5_3:69894339-69894358 Msa0827930:three_prime_UTR 35.0%
CTACTAATGGCAAGCATATT+AGG + chr5_3:69894414-69894433 Msa0827930:three_prime_UTR 35.0%
ATATATTATGCCTACCAGTG+AGG + chr5_3:69894440-69894459 Msa0827930:three_prime_UTR 35.0%
CTTCAGAAGAGAAATGAGAA+AGG + chr5_3:69894571-69894590 Msa0827930:three_prime_UTR 35.0%
TCAGATGCAAGAATTTGAGT+CGG + chr5_3:69892959-69892978 Msa0827930:exon 35.0%
TCTGAATGCAAGATACAATG+TGG + chr5_3:69893096-69893115 Msa0827930:intron 35.0%
CTGAATGCAAGATACAATGT+GGG + chr5_3:69893097-69893116 Msa0827930:intron 35.0%
TGAATGCAAGATACAATGTG+GGG + chr5_3:69893098-69893117 Msa0827930:intron 35.0%
ATAAGAGTGGAATGCATTAG+TGG - chr5_3:69893436-69893455 None:intergenic 35.0%
TTTGTAGCTGGAAATAAGAG+TGG - chr5_3:69893449-69893468 None:intergenic 35.0%
!! AATGGATTAGTCTTTGTAGC+TGG - chr5_3:69893461-69893480 None:intergenic 35.0%
! TGCGGATTTTACGTAAAGAT+GGG - chr5_3:69893578-69893597 None:intergenic 35.0%
! TTGCGGATTTTACGTAAAGA+TGG - chr5_3:69893579-69893598 None:intergenic 35.0%
AAAACATGCCTGATAGTTTG+CGG - chr5_3:69893596-69893615 None:intergenic 35.0%
TCTAATAATTCTGCAGCAGA+AGG - chr5_3:69893699-69893718 None:intergenic 35.0%
! AATAAGGAGTTGATCAGTTG+TGG + chr5_3:69893753-69893772 Msa0827930:CDS 35.0%
! CAGTTGTGGCTTTACTATAT+CGG + chr5_3:69893767-69893786 Msa0827930:CDS 35.0%
!! ACTATATCGGAGGTATCATT+TGG + chr5_3:69893780-69893799 Msa0827930:intron 35.0%
!!! TTTTTTGATCTCATTGCAGG+TGG + chr5_3:69893987-69894006 Msa0827930:intron 35.0%
AAATCTTAAAACACTCGAGG+TGG + chr5_3:69894118-69894137 Msa0827930:three_prime_UTR 35.0%
AATCTTAAAACACTCGAGGT+GGG + chr5_3:69894119-69894138 Msa0827930:three_prime_UTR 35.0%
! GCTAGTTCTCCAAATATTTG+AGG + chr5_3:69894193-69894212 Msa0827930:three_prime_UTR 35.0%
! TCTCCAAATATTTGAGGTAG+CGG + chr5_3:69894199-69894218 Msa0827930:three_prime_UTR 35.0%
TTTCCGCTACCTCAAATATT+TGG - chr5_3:69894205-69894224 None:intergenic 35.0%
! CTGATAATTTTCTGACATGG+AGG + chr5_3:69894249-69894268 Msa0827930:three_prime_UTR 35.0%
! TACTGTTTAGAGCAAACATG+GGG + chr5_3:69894339-69894358 Msa0827930:three_prime_UTR 35.0%
CTACTAATGGCAAGCATATT+AGG + chr5_3:69894414-69894433 Msa0827930:three_prime_UTR 35.0%
ATATATTATGCCTACCAGTG+AGG + chr5_3:69894440-69894459 Msa0827930:three_prime_UTR 35.0%
CTTCAGAAGAGAAATGAGAA+AGG + chr5_3:69894571-69894590 Msa0827930:three_prime_UTR 35.0%
!! GGCTAAATTCAGCATTGATC+AGG + chr5_3:69892980-69892999 Msa0827930:CDS 40.0%
GCAAACTATGGGACAACAAA+TGG - chr5_3:69893046-69893065 None:intergenic 40.0%
GAATGCAAGATACAATGTGG+GGG + chr5_3:69893099-69893118 Msa0827930:intron 40.0%
TTATTTGAGTCCTTGGTCAC+TGG + chr5_3:69893504-69893523 Msa0827930:CDS 40.0%
CGTAAAATCCGCAAACTATC+AGG + chr5_3:69893585-69893604 Msa0827930:CDS 40.0%
TTTATGTAGACACTGTTGGC+CGG + chr5_3:69893631-69893650 Msa0827930:intron 40.0%
ATTCTCCCAAGATCCTGTAA+TGG - chr5_3:69893736-69893755 None:intergenic 40.0%
TACAGGATCTTGGGAGAATA+AGG + chr5_3:69893737-69893756 Msa0827930:CDS 40.0%
! TTGTGGCTTTACTATATCGG+AGG + chr5_3:69893770-69893789 Msa0827930:CDS 40.0%
!! AGAATCATTGGTAATGTGGC+TGG - chr5_3:69893872-69893891 None:intergenic 40.0%
GGAGAATGCATGTGAAGAAA+AGG - chr5_3:69893944-69893963 None:intergenic 40.0%
ATCTTAAAACACTCGAGGTG+GGG + chr5_3:69894120-69894139 Msa0827930:three_prime_UTR 40.0%
!! TAACATGTTTTTGCCTCGGA+GGG - chr5_3:69894167-69894186 None:intergenic 40.0%
!! GGTAGCGGAAAGTTAGTTTA+TGG + chr5_3:69894214-69894233 Msa0827930:three_prime_UTR 40.0%
ACGACAACAAAATCGTCATG+AGG - chr5_3:69894278-69894297 None:intergenic 40.0%
! TTTAGAGCAAACATGGGGAT+GGG + chr5_3:69894344-69894363 Msa0827930:three_prime_UTR 40.0%
CTAAAAATAGCCTCACTGGT+AGG - chr5_3:69894453-69894472 None:intergenic 40.0%
GGATCTAAAAATAGCCTCAC+TGG - chr5_3:69894457-69894476 None:intergenic 40.0%
!! GGCTAAATTCAGCATTGATC+AGG + chr5_3:69892980-69892999 Msa0827930:CDS 40.0%
GCAAACTATGGGACAACAAA+TGG - chr5_3:69893046-69893065 None:intergenic 40.0%
GAATGCAAGATACAATGTGG+GGG + chr5_3:69893099-69893118 Msa0827930:intron 40.0%
TTATTTGAGTCCTTGGTCAC+TGG + chr5_3:69893504-69893523 Msa0827930:CDS 40.0%
CGTAAAATCCGCAAACTATC+AGG + chr5_3:69893585-69893604 Msa0827930:CDS 40.0%
TTTATGTAGACACTGTTGGC+CGG + chr5_3:69893631-69893650 Msa0827930:intron 40.0%
ATTCTCCCAAGATCCTGTAA+TGG - chr5_3:69893736-69893755 None:intergenic 40.0%
TACAGGATCTTGGGAGAATA+AGG + chr5_3:69893737-69893756 Msa0827930:CDS 40.0%
! TTGTGGCTTTACTATATCGG+AGG + chr5_3:69893770-69893789 Msa0827930:CDS 40.0%
!! AGAATCATTGGTAATGTGGC+TGG - chr5_3:69893872-69893891 None:intergenic 40.0%
GGAGAATGCATGTGAAGAAA+AGG - chr5_3:69893944-69893963 None:intergenic 40.0%
ATCTTAAAACACTCGAGGTG+GGG + chr5_3:69894120-69894139 Msa0827930:three_prime_UTR 40.0%
!! TAACATGTTTTTGCCTCGGA+GGG - chr5_3:69894167-69894186 None:intergenic 40.0%
!! GGTAGCGGAAAGTTAGTTTA+TGG + chr5_3:69894214-69894233 Msa0827930:three_prime_UTR 40.0%
ACGACAACAAAATCGTCATG+AGG - chr5_3:69894278-69894297 None:intergenic 40.0%
! TTTAGAGCAAACATGGGGAT+GGG + chr5_3:69894344-69894363 Msa0827930:three_prime_UTR 40.0%
CTAAAAATAGCCTCACTGGT+AGG - chr5_3:69894453-69894472 None:intergenic 40.0%
GGATCTAAAAATAGCCTCAC+TGG - chr5_3:69894457-69894476 None:intergenic 40.0%
! TGATCAGGTTGTCTTCAGTC+TGG + chr5_3:69893207-69893226 Msa0827930:intron 45.0%
AGCTGCAGAAGAGACTTTAC+AGG + chr5_3:69893351-69893370 Msa0827930:CDS 45.0%
ATTTGGATCACCAGTGACCA+AGG - chr5_3:69893517-69893536 None:intergenic 45.0%
GCAGGGAACTCACTCAAATT+TGG - chr5_3:69893534-69893553 None:intergenic 45.0%
GATCTTCAAGAGACTCTGCA+GGG - chr5_3:69893551-69893570 None:intergenic 45.0%
AGATCTTCAAGAGACTCTGC+AGG - chr5_3:69893552-69893571 None:intergenic 45.0%
GCAGCAGAAGGATTAATGAC+AGG - chr5_3:69893687-69893706 None:intergenic 45.0%
ATTAGATGATGATCCCTCCG+AGG + chr5_3:69894151-69894170 Msa0827930:three_prime_UTR 45.0%
!! CTAACATGTTTTTGCCTCGG+AGG - chr5_3:69894168-69894187 None:intergenic 45.0%
! GTTTAGAGCAAACATGGGGA+TGG + chr5_3:69894343-69894362 Msa0827930:three_prime_UTR 45.0%
! TGATCAGGTTGTCTTCAGTC+TGG + chr5_3:69893207-69893226 Msa0827930:intron 45.0%
AGCTGCAGAAGAGACTTTAC+AGG + chr5_3:69893351-69893370 Msa0827930:CDS 45.0%
ATTTGGATCACCAGTGACCA+AGG - chr5_3:69893517-69893536 None:intergenic 45.0%
GCAGGGAACTCACTCAAATT+TGG - chr5_3:69893534-69893553 None:intergenic 45.0%
GATCTTCAAGAGACTCTGCA+GGG - chr5_3:69893551-69893570 None:intergenic 45.0%
AGATCTTCAAGAGACTCTGC+AGG - chr5_3:69893552-69893571 None:intergenic 45.0%
GCAGCAGAAGGATTAATGAC+AGG - chr5_3:69893687-69893706 None:intergenic 45.0%
ATTAGATGATGATCCCTCCG+AGG + chr5_3:69894151-69894170 Msa0827930:three_prime_UTR 45.0%
!! CTAACATGTTTTTGCCTCGG+AGG - chr5_3:69894168-69894187 None:intergenic 45.0%
! GTTTAGAGCAAACATGGGGA+TGG + chr5_3:69894343-69894362 Msa0827930:three_prime_UTR 45.0%
AGCAAACATGGGGATGGGAT+TGG + chr5_3:69894349-69894368 Msa0827930:three_prime_UTR 50.0%
GCAAACATGGGGATGGGATT+GGG + chr5_3:69894350-69894369 Msa0827930:three_prime_UTR 50.0%
CAAACATGGGGATGGGATTG+GGG + chr5_3:69894351-69894370 Msa0827930:three_prime_UTR 50.0%
AGCAAACATGGGGATGGGAT+TGG + chr5_3:69894349-69894368 Msa0827930:three_prime_UTR 50.0%
GCAAACATGGGGATGGGATT+GGG + chr5_3:69894350-69894369 Msa0827930:three_prime_UTR 50.0%
CAAACATGGGGATGGGATTG+GGG + chr5_3:69894351-69894370 Msa0827930:three_prime_UTR 50.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 69892922 69894630 69892922 ID=Msa0827930;Name=Msa0827930
chr5_3 mRNA 69892922 69894630 69892922 ID=Msa0827930-mRNA-1;Parent=Msa0827930;Name=Msa0827930-mRNA-1;_AED=0.00;_eAED=0.00;_QI=41|1|1|1|1|1|5|541|169
chr5_3 exon 69892922 69893001 69892922 ID=Msa0827930-mRNA-1:exon:21902;Parent=Msa0827930-mRNA-1
chr5_3 exon 69893214 69893372 69893214 ID=Msa0827930-mRNA-1:exon:21903;Parent=Msa0827930-mRNA-1
chr5_3 exon 69893462 69893606 69893462 ID=Msa0827930-mRNA-1:exon:21904;Parent=Msa0827930-mRNA-1
chr5_3 exon 69893709 69893791 69893709 ID=Msa0827930-mRNA-1:exon:21905;Parent=Msa0827930-mRNA-1
chr5_3 exon 69894006 69894630 69894006 ID=Msa0827930-mRNA-1:exon:21906;Parent=Msa0827930-mRNA-1
chr5_3 five_prime_UTR 69892922 69892962 69892922 ID=Msa0827930-mRNA-1:five_prime_utr;Parent=Msa0827930-mRNA-1
chr5_3 CDS 69892963 69893001 69892963 ID=Msa0827930-mRNA-1:cds;Parent=Msa0827930-mRNA-1
chr5_3 CDS 69893214 69893372 69893214 ID=Msa0827930-mRNA-1:cds;Parent=Msa0827930-mRNA-1
chr5_3 CDS 69893462 69893606 69893462 ID=Msa0827930-mRNA-1:cds;Parent=Msa0827930-mRNA-1
chr5_3 CDS 69893709 69893791 69893709 ID=Msa0827930-mRNA-1:cds;Parent=Msa0827930-mRNA-1
chr5_3 CDS 69894006 69894089 69894006 ID=Msa0827930-mRNA-1:cds;Parent=Msa0827930-mRNA-1
chr5_3 three_prime_UTR 69894090 69894630 69894090 ID=Msa0827930-mRNA-1:three_prime_utr;Parent=Msa0827930-mRNA-1
Gene Sequence

>Msa0827930

ATGCAAGAATTTGAGTCGGCTAAATTCAGCATTGATCAGGTTGTCTTCAGTCTGGAAAAAGTGCATATCATATGGGAGCCACTTTTGCTTCCTTTAACTTACAAGAAGTGCATGTGTACAGTATTAGAGTCAGTTTTCTCTAGGATTGCAAGAGATATACTTCTTCTAGATGACATAGCTGCAGAAGAGACTTTACAGCTACAAAGACTAATCCATTTAATGCTGGAAAACCTATCATCTTTATTTGAGTCCTTGGTCACTGGTGATCCAAATTTGAGTGAGTTCCCTGCAGAGTCTCTTGAAGATCTTATCCCATCTTTACGTAAAATCCGCAAACTATCAGAATTATTAGATATGCCTTTAAAATCCATTACAGGATCTTGGGAGAATAAGGAGTTGATCAGTTGTGGCTTTACTATATCGGAGGTGGAAGATTTCATAAAAGCTATATTTGCAGACTCACCTTTAAGAAAAGATTGTTTACGGAGGATACAAAATACGAGCTTTTAG
Protein sequence

>Msa0827930

MQEFESAKFSIDQVVFSLEKVHIIWEPLLLPLTYKKCMCTVLESVFSRIARDILLLDDIAAEETLQLQRLIHLMLENLSSLFESLVTGDPNLSEFPAESLEDLIPSLRKIRKLSELLDMPLKSITGSWENKELISCGFTISEVEDFIKAIFADSPLRKDCLRRIQNTSF*