AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0828670

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0828670 MtrunA17_Chr5g0397401 87.850 642 56 2 1 641 1 621 0.0 1048
Msa0828670 MtrunA17_Chr8g0386851 50.736 747 244 21 1 641 1 729 0.0 570
Msa0828670 MtrunA17_Chr4g0032351 30.435 368 196 16 292 640 549 875 5.90e-27 117
Msa0828670 MtrunA17_Chr7g0240931 29.630 297 168 11 292 572 530 801 2.77e-26 114
Msa0828670 MtrunA17_Chr1g0186181 28.947 342 175 11 289 573 264 594 7.67e-26 112
Msa0828670 MtrunA17_Chr2g0328721 30.220 364 182 15 292 640 608 914 1.60e-25 112
Msa0828670 MtrunA17_Chr4g0008861 27.297 370 222 13 215 572 542 876 3.24e-24 108
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0828670 AT4G11450.1 35.616 730 319 24 16 641 12 694 1.40e-93 303
Msa0828670 AT1G63520.1 33.815 346 179 10 284 601 179 502 1.56e-48 178
Msa0828670 AT5G59020.1 29.650 371 190 14 292 640 458 779 7.91e-26 113
Msa0828670 AT2G29510.1 29.380 371 183 16 292 640 525 838 1.25e-22 103
Msa0828670 AT1G04490.9 33.663 202 93 8 391 573 210 389 5.69e-22 99.4
Msa0828670 AT1G04490.5 33.663 202 93 8 391 573 210 389 5.69e-22 99.4
Msa0828670 AT1G04490.6 33.663 202 93 8 391 573 210 389 5.69e-22 99.4
Msa0828670 AT1G04490.3 33.663 202 93 8 391 573 210 389 5.69e-22 99.4
Msa0828670 AT1G04490.7 33.663 202 93 8 391 573 210 389 5.69e-22 99.4
Msa0828670 AT1G04490.2 33.663 202 93 8 391 573 210 389 5.69e-22 99.4
Msa0828670 AT1G04490.1 33.663 202 93 8 391 573 210 389 5.69e-22 99.4
Msa0828670 AT1G04490.8 33.663 202 93 8 391 573 210 389 5.69e-22 99.4
Msa0828670 AT5G01030.3 41.379 116 59 2 457 572 612 718 5.76e-21 98.2
Msa0828670 AT5G01030.1 41.379 116 59 2 457 572 612 718 5.76e-21 98.2
Msa0828670 AT5G01030.2 41.379 116 59 2 457 572 612 718 5.76e-21 98.2
Msa0828670 AT2G33360.2 39.831 118 65 2 459 576 323 434 1.68e-20 95.1
Msa0828670 AT2G33360.1 39.831 118 65 2 459 576 478 589 2.52e-20 95.9
Msa0828670 AT2G37930.1 34.234 111 62 2 461 571 401 500 2.49e-14 76.6
Msa0828670 AT2G37930.3 34.234 111 62 2 461 571 429 528 2.55e-14 76.6
Msa0828670 AT2G37930.4 34.234 111 62 2 461 571 429 528 2.55e-14 76.6
Msa0828670 AT1G04490.4 33.582 134 60 5 391 505 210 333 7.98e-13 70.9

Find 127 sgRNAs with CRISPR-Local

Find 181 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTAATGATTCTCTTGTAAT+TGG 0.204795 5_4:-1516533 Msa0828670:CDS
ATTACAATATAGACAATATT+TGG 0.208992 5_4:+1514144 None:intergenic
GAGTAGGTAATGCTTTAAAT+TGG 0.219018 5_4:-1516136 Msa0828670:CDS
ATTTGTCAATAGAGAAATTA+AGG 0.231675 5_4:-1516285 Msa0828670:CDS
TGTTGGTAGGACCTGAAATT+TGG 0.234521 5_4:-1514392 Msa0828670:three_prime_UTR
CTGATTTGGACTCTGATAAA+TGG 0.241736 5_4:+1516880 None:intergenic
GCATTAGAAGATGGAGTAAT+TGG 0.242621 5_4:-1515990 Msa0828670:CDS
TTCAAAGATTAGAAGTAATA+AGG 0.259001 5_4:-1516555 Msa0828670:CDS
GCACTTTATATGTTCAAATA+AGG 0.264367 5_4:+1514246 None:intergenic
TTTACCTTAAACTCAAAATA+AGG 0.270888 5_4:+1516194 None:intergenic
GAGGTTGCGACTGTGGTGGC+TGG 0.277543 5_4:-1515629 Msa0828670:CDS
GAAGAAAATGGCCACAATTC+AGG 0.288041 5_4:-1516858 Msa0828670:CDS
GCCCGCCCAATAGGAGAAAA+AGG 0.312049 5_4:+1514523 None:intergenic
ATGTTCACTTACCAAATTTC+AGG 0.315147 5_4:+1514381 None:intergenic
TCTAGTCTATGAGATCTAAA+TGG 0.321402 5_4:+1516929 None:intergenic
TTCTTGTTCCTGTGCTTGTA+TGG 0.324223 5_4:-1516672 Msa0828670:CDS
ATTGAATTGTGCATAAATTC+AGG 0.325515 5_4:-1516626 Msa0828670:CDS
TTTGGTGTTACAAATTATCT+TGG 0.330407 5_4:+1514162 None:intergenic
ACCAACAATTGCATACAATA+TGG 0.339112 5_4:+1514408 None:intergenic
ATGAGATGGAAGAACAGAAT+TGG 0.341418 5_4:+1517045 None:intergenic
AGGGAGAAAGCATGTAGAAA+TGG 0.343187 5_4:-1517001 Msa0828670:CDS
CATTCACTCCTATTAGAAAA+AGG 0.353126 5_4:-1516415 Msa0828670:CDS
CAATCACTTTCTGATGGATA+TGG 0.355890 5_4:+1516464 None:intergenic
AAAGGTAGGAGTACTTTCTT+TGG 0.371277 5_4:+1514822 None:intergenic
GAGTCCAAATCAGAAGAAAA+TGG 0.371965 5_4:-1516870 Msa0828670:CDS
ATTGCCAGCTATGAGTTATA+AGG 0.376478 5_4:-1514305 Msa0828670:three_prime_UTR
CTCATAGCTGGCAATATTGA+TGG 0.381576 5_4:+1514313 None:intergenic
TTTGCAACAAAGGCATGTTC+AGG 0.382252 5_4:+1516167 None:intergenic
ACACTTCATGCCCGCCCAAT+AGG 0.384596 5_4:+1514514 None:intergenic
TGGAGTAATTGGAAACTATA+TGG 0.389111 5_4:-1515979 Msa0828670:CDS
AATATTGTCTATATTGTAAT+GGG 0.393251 5_4:-1514141 Msa0828670:three_prime_UTR
GGTAGAGGTGCCATGCAAAA+TGG 0.403175 5_4:+1514714 None:intergenic
AGTAGGTAATGCTTTAAATT+GGG 0.403955 5_4:-1516135 Msa0828670:CDS
GGATGAAATTCACATCAATA+TGG 0.410646 5_4:-1514353 Msa0828670:three_prime_UTR
TCTAGTATAGTAGAAGTAAC+AGG 0.420129 5_4:-1516587 Msa0828670:CDS
ACTAGTGACAACTCTTGTTC+TGG 0.422154 5_4:-1516806 Msa0828670:CDS
GGGAGAAAGCATGTAGAAAT+GGG 0.430696 5_4:-1517000 Msa0828670:CDS
AAACAATAGCAGCACTTTCA+AGG 0.433528 5_4:+1515850 None:intergenic
TATTGTAATGGGATAATAAG+AGG 0.438232 5_4:-1514130 Msa0828670:three_prime_UTR
AAATATTGTCTATATTGTAA+TGG 0.438286 5_4:-1514142 Msa0828670:three_prime_UTR
AACGAAGAATAATGCAGGCT+TGG 0.438605 5_4:-1516084 Msa0828670:CDS
CGACTGTGGTGGCTGGGACA+TGG 0.444536 5_4:-1515622 Msa0828670:CDS
GGCAAACCATGGTTTCCAAT+TGG 0.448117 5_4:+1515705 None:intergenic
CTTCAGCGCTAAGGAAGAAC+AGG 0.449187 5_4:-1514692 Msa0828670:CDS
GAATTGCCTAAAAGAATAAG+AGG 0.449551 5_4:+1515594 None:intergenic
AAAGAAAGTACTCCTACCTT+TGG 0.451706 5_4:-1514820 Msa0828670:CDS
ATTATTGATGGGACAAGAGT+TGG 0.452032 5_4:-1517101 None:intergenic
ATGCAAAATGGCGACGCAAA+TGG 0.453647 5_4:+1514726 None:intergenic
TTGGTGTTACAAATTATCTT+GGG 0.454142 5_4:+1514163 None:intergenic
TCGAGTTGCGGCCTTCAATC+AGG 0.454643 5_4:+1514459 None:intergenic
TGAAGAAATATCACTTGGTA+TGG 0.455014 5_4:+1515755 None:intergenic
TCCATATTGTATGCAATTGT+TGG 0.457862 5_4:-1514409 Msa0828670:three_prime_UTR
GAATGAATAGTCAGCTCTCA+TGG 0.463256 5_4:-1514214 Msa0828670:three_prime_UTR
TGAGATGGAAGAACAGAATT+GGG 0.465267 5_4:+1517046 None:intergenic
TTATGTTCTGCATTAGAAGA+TGG 0.466903 5_4:-1515999 Msa0828670:CDS
AGATTACCATGCAAATGAAC+AGG 0.469037 5_4:+1516242 None:intergenic
GTGAAGGTGGTATTACCAAT+TGG 0.472172 5_4:-1515720 Msa0828670:CDS
ACGAAGAATAATGCAGGCTT+GGG 0.479565 5_4:-1516083 Msa0828670:CDS
CTGTTCTTCCTTAGCGCTGA+AGG 0.482475 5_4:+1514693 None:intergenic
CGCTCACCTGTTCATTTGCA+TGG 0.482795 5_4:-1516248 Msa0828670:CDS
TTCCTTAGCGCTGAAGGTAG+AGG 0.484557 5_4:+1514699 None:intergenic
ACCTTCTTCAGTAATATCTC+AGG 0.485628 5_4:-1516726 Msa0828670:CDS
TCCTGAGATATTACTGAAGA+AGG 0.493642 5_4:+1516725 None:intergenic
AAGCTTTCTCTCTTATGAAA+CGG 0.499315 5_4:+1515819 None:intergenic
TGACAAAGATTCATCATCAA+TGG 0.501640 5_4:-1516048 Msa0828670:CDS
TTCCAACATAGCCTGATTGA+AGG 0.507925 5_4:-1514470 Msa0828670:three_prime_UTR
GGCATGACATTAGTTGAAGA+GGG 0.515356 5_4:-1514799 Msa0828670:CDS
ATGCCTTTGTTGCAAAGACA+TGG 0.516018 5_4:-1516160 Msa0828670:CDS
TCTTCCTTATAACTCATAGC+TGG 0.516464 5_4:+1514301 None:intergenic
AGGTTGCGACTGTGGTGGCT+GGG 0.520329 5_4:-1515628 Msa0828670:CDS
TTGCAACAAAGGCATGTTCA+GGG 0.525799 5_4:+1516168 None:intergenic
GATGAACAATCACTTTCTGA+TGG 0.526712 5_4:+1516458 None:intergenic
CAGTCATAACAGAGGAGAAG+AGG 0.532964 5_4:-1514597 Msa0828670:CDS
TGTGGAGAAATATCAATCAC+TGG 0.535638 5_4:-1515541 Msa0828670:CDS
CACCTCTACCTTCAGCGCTA+AGG 0.536910 5_4:-1514701 Msa0828670:CDS
TATTGTATGCAATTGTTGGT+AGG 0.537280 5_4:-1514405 Msa0828670:three_prime_UTR
ATACAATATGGACTCATAGC+TGG 0.537713 5_4:+1514420 None:intergenic
CTTCATCGTTACTTGATCGA+TGG 0.538533 5_4:-1515662 Msa0828670:CDS
GCCTAAAAGAATAAGAGGGC+AGG 0.547406 5_4:+1515599 None:intergenic
ATTGTAATGGGATAATAAGA+GGG 0.550658 5_4:-1514129 Msa0828670:three_prime_UTR
GAAAGCTTGAAATACAAAAG+AGG 0.552857 5_4:-1515804 Msa0828670:CDS
ATTAGAACGAAGAATAATGC+AGG 0.554547 5_4:-1516089 Msa0828670:CDS
TCATGAAGATAGCCCTCTTG+TGG 0.557960 5_4:-1515559 Msa0828670:CDS
TGGCATGACATTAGTTGAAG+AGG 0.559062 5_4:-1514800 Msa0828670:CDS
GGCCTTCAATCAGGCTATGT+TGG 0.563061 5_4:+1514468 None:intergenic
CATGGTGGAGGTTGCGACTG+TGG 0.566215 5_4:-1515636 Msa0828670:CDS
TCATAGCTGGCAATATTGAT+GGG 0.570259 5_4:+1514314 None:intergenic
GTATTACCAATTGGAAACCA+TGG 0.570636 5_4:-1515711 Msa0828670:CDS
GATTGATATTTCTCCACAAG+AGG 0.571910 5_4:+1515546 None:intergenic
AAACTGTTTGAATGTGAAGA+GGG 0.583491 5_4:-1517020 Msa0828670:CDS
AAAACTGTTTGAATGTGAAG+AGG 0.584549 5_4:-1517021 Msa0828670:CDS
GCACAGGAACAAGAAACAGA+TGG 0.584959 5_4:+1516680 None:intergenic
AAGAACTAGAGCATCTTCTA+CGG 0.584988 5_4:+1514269 None:intergenic
AAAAGATAGAAATTTCAAGT+AGG 0.590011 5_4:-1516829 Msa0828670:CDS
AAAAGGCGGGTTCAGCATAG+AGG 0.592188 5_4:+1514540 None:intergenic
GAGAATATAGAAACTAGAAG+AGG 0.592815 5_4:-1516905 Msa0828670:CDS
GCTGTGATCCATACAAGCAC+AGG 0.592937 5_4:+1516664 None:intergenic
TTCAACTAATGTCATGCCAA+AGG 0.596988 5_4:+1514804 None:intergenic
TTGTGTGGTTCAGATGATGA+AGG 0.597591 5_4:-1516767 Msa0828670:CDS
AAATGGCCACAATTCAGGAA+AGG 0.598724 5_4:-1516853 Msa0828670:CDS
TTTCAGCACTTGGCAAACCA+TGG 0.600295 5_4:+1515694 None:intergenic
CACTTTCTGATGGATATGGT+TGG 0.600468 5_4:+1516468 None:intergenic
GTTGCAAAGACATGGAGAGT+AGG 0.601872 5_4:-1516152 Msa0828670:CDS
ACTAATGTCATGCCAAAGGT+AGG 0.602147 5_4:+1514808 None:intergenic
CATCGATCAAGTAACGATGA+AGG 0.603033 5_4:+1515663 None:intergenic
AGATGATGAAGGTTCTGTTG+AGG 0.604425 5_4:-1516756 Msa0828670:CDS
GTCTATGAGATCTAAATGGA+AGG 0.606001 5_4:+1516933 None:intergenic
TTACTTGATCGATGGAAACA+TGG 0.608456 5_4:-1515654 Msa0828670:CDS
TCTCCATGTCTTTGCAACAA+AGG 0.610158 5_4:+1516157 None:intergenic
TGCAACAAAGGCATGTTCAG+GGG 0.610690 5_4:+1516169 None:intergenic
TTCACTAGTAGAGCAAGTGA+AGG 0.612894 5_4:-1515736 Msa0828670:CDS
ATTGATATTTCTCCACAAGA+GGG 0.615796 5_4:+1515547 None:intergenic
TTTGCCAAGTGCTGAAAGCG+AGG 0.618483 5_4:-1515688 Msa0828670:CDS
TGAAATTTGGTAAGTGAACA+TGG 0.619849 5_4:-1514379 Msa0828670:three_prime_UTR
ACTAGTAGAGCAAGTGAAGG+TGG 0.627677 5_4:-1515733 Msa0828670:CDS
GCATGACATTAGTTGAAGAG+GGG 0.628529 5_4:-1514798 Msa0828670:CDS
AATTGCCTAAAAGAATAAGA+GGG 0.636194 5_4:+1515595 None:intergenic
ACTAGTGAAGAAATATCACT+TGG 0.637185 5_4:+1515750 None:intergenic
GACAGTGTCTAATATTCACA+AGG 0.640826 5_4:-1514575 Msa0828670:CDS
TTGCCAAGTGCTGAAAGCGA+GGG 0.650835 5_4:-1515687 Msa0828670:CDS
TTTGGTAAGTGAACATGGTG+AGG 0.652629 5_4:-1514374 Msa0828670:three_prime_UTR
TAGAAATGGGAAATTCAATG+AGG 0.676384 5_4:-1516987 Msa0828670:CDS
GGTGGAGGTTGCGACTGTGG+TGG 0.681780 5_4:-1515633 Msa0828670:CDS
GATCGATGGAAACATGGTGG+AGG 0.686983 5_4:-1515648 Msa0828670:CDS
CTTGATCGATGGAAACATGG+TGG 0.691146 5_4:-1515651 Msa0828670:CDS
CATCAAATCAGTCATAACAG+AGG 0.691271 5_4:-1514605 Msa0828670:CDS
ACAGTGTCTAATATTCACAA+GGG 0.748410 5_4:-1514574 Msa0828670:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ACTTACATTTTATCTATTTT+TGG - chr5_4:1516354-1516373 Msa0828670:CDS 15.0%
!! ATTACAATATAGACAATATT+TGG + chr5_4:1517069-1517088 None:intergenic 15.0%
!! AAATATTGTCTATATTGTAA+TGG - chr5_4:1517068-1517087 Msa0828670:CDS 15.0%
!! AATATTGTCTATATTGTAAT+GGG - chr5_4:1517069-1517088 Msa0828670:CDS 15.0%
!!! AAAAGATAGAAATTTCAAGT+AGG - chr5_4:1514381-1514400 Msa0828670:three_prime_UTR 20.0%
!! TTCAAAGATTAGAAGTAATA+AGG - chr5_4:1514655-1514674 Msa0828670:CDS 20.0%
!! ATTTGTCAATAGAGAAATTA+AGG - chr5_4:1514925-1514944 Msa0828670:intron 20.0%
!! TTTACCTTAAACTCAAAATA+AGG + chr5_4:1515019-1515038 None:intergenic 20.0%
!!! TGCAATTGTTTTATAAAATG+TGG + chr5_4:1516026-1516045 None:intergenic 20.0%
!!! TTTTGTTGAATACAAATAAC+AGG + chr5_4:1516120-1516139 None:intergenic 20.0%
!! GTACTATAAAAAACAATAAG+AGG + chr5_4:1516336-1516355 None:intergenic 20.0%
! ATTGAATTGTGCATAAATTC+AGG - chr5_4:1514584-1514603 Msa0828670:CDS 25.0%
! GTTAATGATTCTCTTGTAAT+TGG - chr5_4:1514677-1514696 Msa0828670:CDS 25.0%
!!! ACTTTTTTTGACTTTGTGAA+AGG + chr5_4:1514827-1514846 None:intergenic 25.0%
! AAAAAAAGTTGCAAGAAACA+TGG - chr5_4:1514838-1514857 Msa0828670:intron 25.0%
!!! ACATCCTTATTTTGAGTTTA+AGG - chr5_4:1515012-1515031 Msa0828670:intron 25.0%
!! AGTAGGTAATGCTTTAAATT+GGG - chr5_4:1515075-1515094 Msa0828670:intron 25.0%
! AATTGCCTAAAAGAATAAGA+GGG + chr5_4:1515618-1515637 None:intergenic 25.0%
! TGAAATTTAATCTTTACCTG+AGG + chr5_4:1515709-1515728 None:intergenic 25.0%
! TCATATAAAGTCTTTCTTGA+AGG - chr5_4:1515726-1515745 Msa0828670:CDS 25.0%
! TTCTTATTAAATTCGAGAAC+TGG + chr5_4:1515774-1515793 None:intergenic 25.0%
! ATTATGACGATTTAATCGAT+TGG + chr5_4:1515968-1515987 None:intergenic 25.0%
! TCATAGAATGCATATACAAT+CGG - chr5_4:1516167-1516186 Msa0828670:CDS 25.0%
!! AAAAAGTTAGTTAGCCTAAT+TGG - chr5_4:1516200-1516219 Msa0828670:CDS 25.0%
!!! TACAACAATGTGTTTTTGAT+TGG - chr5_4:1516261-1516280 Msa0828670:CDS 25.0%
!! GTAATGAAGATAATTTTGCA+TGG + chr5_4:1516455-1516474 None:intergenic 25.0%
! GCACTTTATATGTTCAAATA+AGG + chr5_4:1516967-1516986 None:intergenic 25.0%
!!! TTGGTGTTACAAATTATCTT+GGG + chr5_4:1517050-1517069 None:intergenic 25.0%
!!! TTTGGTGTTACAAATTATCT+TGG + chr5_4:1517051-1517070 None:intergenic 25.0%
!! TATTGTAATGGGATAATAAG+AGG - chr5_4:1517080-1517099 Msa0828670:CDS 25.0%
!! ATTGTAATGGGATAATAAGA+GGG - chr5_4:1517081-1517100 Msa0828670:CDS 25.0%
!! TTCAAACAGTTTTGATGAGA+TGG + chr5_4:1514182-1514201 None:intergenic 30.0%
AAAACTGTTTGAATGTGAAG+AGG - chr5_4:1514189-1514208 Msa0828670:three_prime_UTR 30.0%
AAACTGTTTGAATGTGAAGA+GGG - chr5_4:1514190-1514209 Msa0828670:three_prime_UTR 30.0%
TAGAAATGGGAAATTCAATG+AGG - chr5_4:1514223-1514242 Msa0828670:three_prime_UTR 30.0%
TCTAGTCTATGAGATCTAAA+TGG + chr5_4:1514284-1514303 None:intergenic 30.0%
GAGAATATAGAAACTAGAAG+AGG - chr5_4:1514305-1514324 Msa0828670:three_prime_UTR 30.0%
!!! AGCATTTTTTATGCATTGTG+TGG - chr5_4:1514428-1514447 Msa0828670:three_prime_UTR 30.0%
TCTAGTATAGTAGAAGTAAC+AGG - chr5_4:1514623-1514642 Msa0828670:CDS 30.0%
TAATTCTCCTCTTCAAAAAG+TGG - chr5_4:1514703-1514722 Msa0828670:CDS 30.0%
CATTCACTCCTATTAGAAAA+AGG - chr5_4:1514795-1514814 Msa0828670:CDS 30.0%
!! GGATTTAGCCTTTTTCTAAT+AGG + chr5_4:1514806-1514825 None:intergenic 30.0%
!! CTCAAGTTGAAAAACAAACA+AGG - chr5_4:1514989-1515008 Msa0828670:intron 30.0%
! GAGTAGGTAATGCTTTAAAT+TGG - chr5_4:1515074-1515093 Msa0828670:intron 30.0%
ATTAGAACGAAGAATAATGC+AGG - chr5_4:1515121-1515140 Msa0828670:intron 30.0%
TGACAAAGATTCATCATCAA+TGG - chr5_4:1515162-1515181 Msa0828670:intron 30.0%
TTATGTTCTGCATTAGAAGA+TGG - chr5_4:1515211-1515230 Msa0828670:intron 30.0%
TGGAGTAATTGGAAACTATA+TGG - chr5_4:1515231-1515250 Msa0828670:intron 30.0%
AAGCTTTCTCTCTTATGAAA+CGG + chr5_4:1515394-1515413 None:intergenic 30.0%
GAAAGCTTGAAATACAAAAG+AGG - chr5_4:1515406-1515425 Msa0828670:intron 30.0%
TGAAGAAATATCACTTGGTA+TGG + chr5_4:1515458-1515477 None:intergenic 30.0%
ACTAGTGAAGAAATATCACT+TGG + chr5_4:1515463-1515482 None:intergenic 30.0%
GAATTGCCTAAAAGAATAAG+AGG + chr5_4:1515619-1515638 None:intergenic 30.0%
ATTGATATTTCTCCACAAGA+GGG + chr5_4:1515666-1515685 None:intergenic 30.0%
ATCGTGAAAAAATCAGTACA+GGG + chr5_4:1515914-1515933 None:intergenic 30.0%
AATCGTGAAAAAATCAGTAC+AGG + chr5_4:1515915-1515934 None:intergenic 30.0%
! TCACGATTTAAAAGCATGTT+TGG - chr5_4:1515927-1515946 Msa0828670:CDS 30.0%
TATCATCATATGAGAAGAGA+AGG + chr5_4:1516003-1516022 None:intergenic 30.0%
GAACATTCTTCAAACTAACT+AGG - chr5_4:1516085-1516104 Msa0828670:CDS 30.0%
!!! TTTATCTATTTTTGGTGCAC+AGG - chr5_4:1516362-1516381 Msa0828670:CDS 30.0%
!!! TTATCTATTTTTGGTGCACA+GGG - chr5_4:1516363-1516382 Msa0828670:CDS 30.0%
ACAGTGTCTAATATTCACAA+GGG - chr5_4:1516636-1516655 Msa0828670:CDS 30.0%
TCCATATTGTATGCAATTGT+TGG - chr5_4:1516801-1516820 Msa0828670:CDS 30.0%
ACCAACAATTGCATACAATA+TGG + chr5_4:1516805-1516824 None:intergenic 30.0%
TATTGTATGCAATTGTTGGT+AGG - chr5_4:1516805-1516824 Msa0828670:CDS 30.0%
ATGTTCACTTACCAAATTTC+AGG + chr5_4:1516832-1516851 None:intergenic 30.0%
TGAAATTTGGTAAGTGAACA+TGG - chr5_4:1516831-1516850 Msa0828670:CDS 30.0%
GGATGAAATTCACATCAATA+TGG - chr5_4:1516857-1516876 Msa0828670:CDS 30.0%
TGAGATGGAAGAACAGAATT+GGG + chr5_4:1514167-1514186 None:intergenic 35.0%
ATGAGATGGAAGAACAGAAT+TGG + chr5_4:1514168-1514187 None:intergenic 35.0%
GTCTATGAGATCTAAATGGA+AGG + chr5_4:1514280-1514299 None:intergenic 35.0%
!! CTGATTTGGACTCTGATAAA+TGG + chr5_4:1514333-1514352 None:intergenic 35.0%
GAGTCCAAATCAGAAGAAAA+TGG - chr5_4:1514340-1514359 Msa0828670:three_prime_UTR 35.0%
! CTTTTTCCTTTCCTGAATTG+TGG + chr5_4:1514366-1514385 None:intergenic 35.0%
ACCTTCTTCAGTAATATCTC+AGG - chr5_4:1514484-1514503 Msa0828670:three_prime_UTR 35.0%
TCCTGAGATATTACTGAAGA+AGG + chr5_4:1514488-1514507 None:intergenic 35.0%
!!! GAGATTTCCACTTTTTGAAG+AGG + chr5_4:1514713-1514732 None:intergenic 35.0%
! CAATCACTTTCTGATGGATA+TGG + chr5_4:1514749-1514768 None:intergenic 35.0%
GATGAACAATCACTTTCTGA+TGG + chr5_4:1514755-1514774 None:intergenic 35.0%
AGATTACCATGCAAATGAAC+AGG + chr5_4:1514971-1514990 None:intergenic 35.0%
GCATTAGAAGATGGAGTAAT+TGG - chr5_4:1515220-1515239 Msa0828670:intron 35.0%
!! TGGCTGTAAGACTTTTTCTT+TGG + chr5_4:1515307-1515326 None:intergenic 35.0%
AAACAATAGCAGCACTTTCA+AGG + chr5_4:1515363-1515382 None:intergenic 35.0%
GTATTACCAATTGGAAACCA+TGG - chr5_4:1515499-1515518 Msa0828670:intron 35.0%
TTACTTGATCGATGGAAACA+TGG - chr5_4:1515556-1515575 Msa0828670:CDS 35.0%
GATTGATATTTCTCCACAAG+AGG + chr5_4:1515667-1515686 None:intergenic 35.0%
TGTGGAGAAATATCAATCAC+TGG - chr5_4:1515669-1515688 Msa0828670:CDS 35.0%
CTTTCTTGAAGGAACTTAGA+TGG - chr5_4:1515737-1515756 Msa0828670:CDS 35.0%
TATTCTTAAATGCGCGTTCA+TGG + chr5_4:1515873-1515892 None:intergenic 35.0%
GAAGAATGTTCTAACTACTG+CGG + chr5_4:1516076-1516095 None:intergenic 35.0%
!! AAAGGTAGGAGTACTTTCTT+TGG + chr5_4:1516391-1516410 None:intergenic 35.0%
!! AAAGAAAGTACTCCTACCTT+TGG - chr5_4:1516390-1516409 Msa0828670:CDS 35.0%
TTCAACTAATGTCATGCCAA+AGG + chr5_4:1516409-1516428 None:intergenic 35.0%
CATCAAATCAGTCATAACAG+AGG - chr5_4:1516605-1516624 Msa0828670:CDS 35.0%
GACAGTGTCTAATATTCACA+AGG - chr5_4:1516635-1516654 Msa0828670:CDS 35.0%
!! AAATATTTTCGCTCGAGTTG+CGG + chr5_4:1516766-1516785 None:intergenic 35.0%
ATACAATATGGACTCATAGC+TGG + chr5_4:1516793-1516812 None:intergenic 35.0%
TCATAGCTGGCAATATTGAT+GGG + chr5_4:1516899-1516918 None:intergenic 35.0%
ATTGCCAGCTATGAGTTATA+AGG - chr5_4:1516905-1516924 Msa0828670:CDS 35.0%
TCTTCCTTATAACTCATAGC+TGG + chr5_4:1516912-1516931 None:intergenic 35.0%
! AAGAACTAGAGCATCTTCTA+CGG + chr5_4:1516944-1516963 None:intergenic 35.0%
! TGGAAAAAATGTGGAGTATC+TGG - chr5_4:1517016-1517035 Msa0828670:CDS 35.0%
! AGGGAGAAAGCATGTAGAAA+TGG - chr5_4:1514209-1514228 Msa0828670:three_prime_UTR 40.0%
! GGGAGAAAGCATGTAGAAAT+GGG - chr5_4:1514210-1514229 Msa0828670:three_prime_UTR 40.0%
!! GTGGCCATTTTCTTCTGATT+TGG + chr5_4:1514347-1514366 None:intergenic 40.0%
GAAGAAAATGGCCACAATTC+AGG - chr5_4:1514352-1514371 Msa0828670:three_prime_UTR 40.0%
AAATGGCCACAATTCAGGAA+AGG - chr5_4:1514357-1514376 Msa0828670:three_prime_UTR 40.0%
ACTAGTGACAACTCTTGTTC+TGG - chr5_4:1514404-1514423 Msa0828670:three_prime_UTR 40.0%
TTGTGTGGTTCAGATGATGA+AGG - chr5_4:1514443-1514462 Msa0828670:three_prime_UTR 40.0%
AGATGATGAAGGTTCTGTTG+AGG - chr5_4:1514454-1514473 Msa0828670:three_prime_UTR 40.0%
TTCTTGTTCCTGTGCTTGTA+TGG - chr5_4:1514538-1514557 Msa0828670:CDS 40.0%
! CACTTTCTGATGGATATGGT+TGG + chr5_4:1514745-1514764 None:intergenic 40.0%
!! GACAGCATCTTTTGTTGTGT+TGG + chr5_4:1514894-1514913 None:intergenic 40.0%
! TTGCAACAAAGGCATGTTCA+GGG + chr5_4:1515045-1515064 None:intergenic 40.0%
! TTTGCAACAAAGGCATGTTC+AGG + chr5_4:1515046-1515065 None:intergenic 40.0%
ATGCCTTTGTTGCAAAGACA+TGG - chr5_4:1515050-1515069 Msa0828670:intron 40.0%
TCTCCATGTCTTTGCAACAA+AGG + chr5_4:1515056-1515075 None:intergenic 40.0%
AACGAAGAATAATGCAGGCT+TGG - chr5_4:1515126-1515145 Msa0828670:intron 40.0%
ACGAAGAATAATGCAGGCTT+GGG - chr5_4:1515127-1515146 Msa0828670:intron 40.0%
!! GAGTTTTCGAAGCATCTTGT+TGG + chr5_4:1515327-1515346 None:intergenic 40.0%
TTCACTAGTAGAGCAAGTGA+AGG - chr5_4:1515474-1515493 Msa0828670:intron 40.0%
GTGAAGGTGGTATTACCAAT+TGG - chr5_4:1515490-1515509 Msa0828670:intron 40.0%
CATCGATCAAGTAACGATGA+AGG + chr5_4:1515550-1515569 None:intergenic 40.0%
CTTCATCGTTACTTGATCGA+TGG - chr5_4:1515548-1515567 Msa0828670:CDS 40.0%
CAATCCTATAGTATGTACGC+AGG + chr5_4:1516236-1516255 None:intergenic 40.0%
ACTAATGTCATGCCAAAGGT+AGG + chr5_4:1516405-1516424 None:intergenic 40.0%
TGGCATGACATTAGTTGAAG+AGG - chr5_4:1516410-1516429 Msa0828670:CDS 40.0%
GGCATGACATTAGTTGAAGA+GGG - chr5_4:1516411-1516430 Msa0828670:CDS 40.0%
GCATGACATTAGTTGAAGAG+GGG - chr5_4:1516412-1516431 Msa0828670:CDS 40.0%
TTCCAACATAGCCTGATTGA+AGG - chr5_4:1516740-1516759 Msa0828670:CDS 40.0%
! TGTTGGTAGGACCTGAAATT+TGG - chr5_4:1516818-1516837 Msa0828670:CDS 40.0%
TTTGGTAAGTGAACATGGTG+AGG - chr5_4:1516836-1516855 Msa0828670:CDS 40.0%
CTCATAGCTGGCAATATTGA+TGG + chr5_4:1516900-1516919 None:intergenic 40.0%
GAATGAATAGTCAGCTCTCA+TGG - chr5_4:1516996-1517015 Msa0828670:CDS 40.0%
CAGCTCTCATGGAAAAAATG+TGG - chr5_4:1517007-1517026 Msa0828670:CDS 40.0%
GCACAGGAACAAGAAACAGA+TGG + chr5_4:1514533-1514552 None:intergenic 45.0%
! TGCAACAAAGGCATGTTCAG+GGG + chr5_4:1515044-1515063 None:intergenic 45.0%
GTTGCAAAGACATGGAGAGT+AGG - chr5_4:1515058-1515077 Msa0828670:intron 45.0%
ACTAGTAGAGCAAGTGAAGG+TGG - chr5_4:1515477-1515496 Msa0828670:intron 45.0%
GGCAAACCATGGTTTCCAAT+TGG + chr5_4:1515508-1515527 None:intergenic 45.0%
TTTCAGCACTTGGCAAACCA+TGG + chr5_4:1515519-1515538 None:intergenic 45.0%
CTTGATCGATGGAAACATGG+TGG - chr5_4:1515559-1515578 Msa0828670:CDS 45.0%
! GCCTGCCCTCTTATTCTTTT+AGG - chr5_4:1515610-1515629 Msa0828670:CDS 45.0%
GCCTAAAAGAATAAGAGGGC+AGG + chr5_4:1515614-1515633 None:intergenic 45.0%
TCATGAAGATAGCCCTCTTG+TGG - chr5_4:1515651-1515670 Msa0828670:CDS 45.0%
GCGCGTTCATGGTATTTGAT+AGG + chr5_4:1515862-1515881 None:intergenic 45.0%
! ATCCAGTCAGCGCTGAATTA+TGG - chr5_4:1516048-1516067 Msa0828670:CDS 45.0%
!! TTCCATAATTCAGCGCTGAC+TGG + chr5_4:1516053-1516072 None:intergenic 45.0%
!! CAGGAGCTTGTTGTCCAATT+AGG + chr5_4:1516217-1516236 None:intergenic 45.0%
AGCTCCTGCGTACATACTAT+AGG - chr5_4:1516229-1516248 Msa0828670:CDS 45.0%
ATGCAAAATGGCGACGCAAA+TGG + chr5_4:1516487-1516506 None:intergenic 45.0%
!! ATTTGCGTCGCCATTTTGCA+TGG - chr5_4:1516486-1516505 Msa0828670:CDS 45.0%
CAGTCATAACAGAGGAGAAG+AGG - chr5_4:1516613-1516632 Msa0828670:CDS 45.0%
! AACCCGCCTTTTTCTCCTAT+TGG - chr5_4:1516681-1516700 Msa0828670:CDS 45.0%
! ACCCGCCTTTTTCTCCTATT+GGG - chr5_4:1516682-1516701 Msa0828670:CDS 45.0%
GCTGTGATCCATACAAGCAC+AGG + chr5_4:1514549-1514568 None:intergenic 50.0%
CGCTCACCTGTTCATTTGCA+TGG - chr5_4:1514962-1514981 Msa0828670:intron 50.0%
!! TTTGCCAAGTGCTGAAAGCG+AGG - chr5_4:1515522-1515541 Msa0828670:CDS 50.0%
!! TTGCCAAGTGCTGAAAGCGA+GGG - chr5_4:1515523-1515542 Msa0828670:CDS 50.0%
GATCGATGGAAACATGGTGG+AGG - chr5_4:1515562-1515581 Msa0828670:CDS 50.0%
! GGAACTTTTCTCTCAGCCTC+AGG - chr5_4:1515690-1515709 Msa0828670:CDS 50.0%
!! GGTAGAGGTGCCATGCAAAA+TGG + chr5_4:1516499-1516518 None:intergenic 50.0%
! TTCCTTAGCGCTGAAGGTAG+AGG + chr5_4:1516514-1516533 None:intergenic 50.0%
! CTGTTCTTCCTTAGCGCTGA+AGG + chr5_4:1516520-1516539 None:intergenic 50.0%
CTTCAGCGCTAAGGAAGAAC+AGG - chr5_4:1516518-1516537 Msa0828670:CDS 50.0%
! AAAAGGCGGGTTCAGCATAG+AGG + chr5_4:1516673-1516692 None:intergenic 50.0%
GCCCAATAGGAGAAAAAGGC+GGG + chr5_4:1516686-1516705 None:intergenic 50.0%
CGCCCAATAGGAGAAAAAGG+CGG + chr5_4:1516687-1516706 None:intergenic 50.0%
! CGCCTTTTTCTCCTATTGGG+CGG - chr5_4:1516685-1516704 Msa0828670:CDS 50.0%
! GCCTTTTTCTCCTATTGGGC+GGG - chr5_4:1516686-1516705 Msa0828670:CDS 50.0%
GGCCTTCAATCAGGCTATGT+TGG + chr5_4:1516745-1516764 None:intergenic 50.0%
CACCTCTACCTTCAGCGCTA+AGG - chr5_4:1516509-1516528 Msa0828670:CDS 55.0%
GCCCGCCCAATAGGAGAAAA+AGG + chr5_4:1516690-1516709 None:intergenic 55.0%
ACACTTCATGCCCGCCCAAT+AGG + chr5_4:1516699-1516718 None:intergenic 55.0%
! TCGAGTTGCGGCCTTCAATC+AGG + chr5_4:1516754-1516773 None:intergenic 55.0%
GGACCCTCGCTTTCAGCACT+TGG + chr5_4:1515529-1515548 None:intergenic 60.0%
! CATGGTGGAGGTTGCGACTG+TGG - chr5_4:1515574-1515593 Msa0828670:CDS 60.0%
AGGTTGCGACTGTGGTGGCT+GGG - chr5_4:1515582-1515601 Msa0828670:CDS 60.0%
GGTGGAGGTTGCGACTGTGG+TGG - chr5_4:1515577-1515596 Msa0828670:CDS 65.0%
GAGGTTGCGACTGTGGTGGC+TGG - chr5_4:1515581-1515600 Msa0828670:CDS 65.0%
CGACTGTGGTGGCTGGGACA+TGG - chr5_4:1515588-1515607 Msa0828670:CDS 65.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 1514116 1517116 1514116 ID=Msa0828670;Name=Msa0828670
chr5_4 mRNA 1514116 1517116 1514116 ID=Msa0828670-mRNA-1;Parent=Msa0828670;Name=Msa0828670-mRNA-1;_AED=0.27;_eAED=0.27;_QI=0|1|0.5|1|1|1|2|403|641
chr5_4 exon 1514116 1514848 1514116 ID=Msa0828670-mRNA-1:exon:390;Parent=Msa0828670-mRNA-1
chr5_4 exon 1515521 1517116 1515521 ID=Msa0828670-mRNA-1:exon:389;Parent=Msa0828670-mRNA-1
chr5_4 CDS 1515521 1517116 1515521 ID=Msa0828670-mRNA-1:cds;Parent=Msa0828670-mRNA-1
chr5_4 CDS 1514519 1514848 1514519 ID=Msa0828670-mRNA-1:cds;Parent=Msa0828670-mRNA-1
chr5_4 three_prime_UTR 1514116 1514518 1514116 ID=Msa0828670-mRNA-1:three_prime_utr;Parent=Msa0828670-mRNA-1
Gene Sequence

>Msa0828670

ATGGGACAAGAGTTGGATCTCAATGACAAGTCTTCTGTGAGTCTCAGTCCCAATTCTGTTCTTCCATCTCATCAAAACTGTTTGAATGTGAAGAGGGAGAAAGCATGTAGAAATGGGAAATTCAATGAGGACTTTGCTGAGATCAATTCTTCATGCAAGAGCCTTCCATTTAGATCTCATAGACTAGAAGAGAATATAGAAACTAGAAGAGGTTCCATTTATCAGAGTCCAAATCAGAAGAAAATGGCCACAATTCAGGAAAGGAAAAAGATAGAAATTTCAAGTAGGACTAGTGACAACTCTTGTTCTGGTAGCATTTTTTATGCATTGTGTGGTTCAGATGATGAAGGTTCTGTTGAGGTTGAGACACCTTCTTCAGTAATATCTCAGGATTCAGAATTGATTTCTCAATCTCCATCTGTTTCTTGTTCCTGTGCTTGTATGGATCACAGCATATCAAGTAGTTTTATTGAATTGTGCATAAATTCAGGTGTTTGTGATAAAAAATCTAGTATAGTAGAAGTAACAGGTTCTGTAAATTCAAAGATTAGAAGTAATAAGGTTAATGATTCTCTTGTAATTGGTAATAATTCTCCTCTTCAAAAAGTGGAAATCTCTCACTCTCACCAACCATATCCATCAGAAAGTGATTGTTCATCAAGAGTGAGTTCAAAAGTCTCATTCACTCCTATTAGAAAAAGGCTAAATCCTTTCACAAAGTCAAAAAAAGTTGCAAGAAACATGGCTTGTCAAAAATCTTTGTTGAATGACTTTTCCAACACAACAAAAGATGCTGTCATTGTTTCAGAATTTGTCAATAGAGAAATTAAGGATTCTAGTCTAGCACGCTCACCTGTTCATTTGCATGGTAATCTCAAGTTGAAAAACAAACAAGGACATCCTTATTTTGAGTTTAAGGTAAATTCCCCTGAACATGCCTTTGTTGCAAAGACATGGAGAGTAGGTAATGCTTTAAATTGGGTATATACATTTCACTCTGTTGATATTAGAACGAAGAATAATGCAGGCTTGGGAACTCAACATTGTGACAAAGATTCATCATCAATGGTAGCACAGATGCTAGTTTCTTGTAGTTTATGTTCTGCATTAGAAGATGGAGTAATTGGAAACTATATGGTTACAGAATTTGTGTTGTATGATTTTACTCATTCAAGAAAAAGTGTTATGCCAAAGAAAAAGTCTTACAGCCAACAAGATGCTTCGAAAACTCTGAATGCTTCTAGCCTTGAAAGTGCTGCTATTGTTTTGCGAATACCGTTTCATAAGAGAGAAAGCTTGAAATACAAAAGAGGTGATAGAACGAATACTAAAGCATATTCCATACCAAGTGATATTTCTTCACTAGTAGAGCAAGTGAAGGTGGTATTACCAATTGGAAACCATGGTTTGCCAAGTGCTGAAAGCGAGGGTCCTTCATCGTTACTTGATCGATGGAAACATGGTGGAGGTTGCGACTGTGGTGGCTGGGACATGGCCTGCCCTCTTATTCTTTTAGGCAATTCTAGTGTTCAGTTTCATGAAGATAGCCCTCTTGTGGAGAAATATCAATCACTGGAACTTTTCTCTCAGCCTCAGGGAACCAAAGAAAGTACTCCTACCTTTGGCATGACATTAGTTGAAGAGGGGCAATATGCAGTTGATTTCCATGCAAAATTATCTTCATTACAAGCTTTCTCCATTTGCGTCGCCATTTTGCATGGCACCTCTACCTTCAGCGCTAAGGAAGAACAGGCGAAAAATCAACAGTTATCTCAATGCAGTTCGTTAAATACACTTATTGAAGAAGTAGAATCATTCATCAAATCAGTCATAACAGAGGAGAAGAGGACAGTGTCTAATATTCACAAGGGAATTCATCGATCCTCTATGCTGAACCCGCCTTTTTCTCCTATTGGGCGGGCATGA
Protein sequence

>Msa0828670

MGQELDLNDKSSVSLSPNSVLPSHQNCLNVKREKACRNGKFNEDFAEINSSCKSLPFRSHRLEENIETRRGSIYQSPNQKKMATIQERKKIEISSRTSDNSCSGSIFYALCGSDDEGSVEVETPSSVISQDSELISQSPSVSCSCACMDHSISSSFIELCINSGVCDKKSSIVEVTGSVNSKIRSNKVNDSLVIGNNSPLQKVEISHSHQPYPSESDCSSRVSSKVSFTPIRKRLNPFTKSKKVARNMACQKSLLNDFSNTTKDAVIVSEFVNREIKDSSLARSPVHLHGNLKLKNKQGHPYFEFKVNSPEHAFVAKTWRVGNALNWVYTFHSVDIRTKNNAGLGTQHCDKDSSSMVAQMLVSCSLCSALEDGVIGNYMVTEFVLYDFTHSRKSVMPKKKSYSQQDASKTLNASSLESAAIVLRIPFHKRESLKYKRGDRTNTKAYSIPSDISSLVEQVKVVLPIGNHGLPSAESEGPSSLLDRWKHGGGCDCGGWDMACPLILLGNSSVQFHEDSPLVEKYQSLELFSQPQGTKESTPTFGMTLVEEGQYAVDFHAKLSSLQAFSICVAILHGTSTFSAKEEQAKNQQLSQCSSLNTLIEEVESFIKSVITEEKRTVSNIHKGIHRSSMLNPPFSPIGRA*