AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0833340

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0833340 MtrunA17_Chr5g0398651 94.828 116 6 0 1 116 1 116 3.38e-77 223
Msa0833340 MtrunA17_Chr4g0056791 63.303 109 35 1 5 113 41 144 1.15e-40 132
Msa0833340 MtrunA17_Chr2g0320881 53.763 93 43 0 20 112 51 143 3.00e-26 97.1
Msa0833340 MtrunA17_Chr4g0027171 50.562 89 44 0 24 112 67 155 1.08e-25 95.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0833340 AT2G27830.1 52.128 94 45 0 19 112 61 154 2.66e-30 107

Find 70 sgRNAs with CRISPR-Local

Find 186 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGGGTAACTAAATATAATT+TGG 0.142333 5_4:-6500841 None:intergenic
AAGATATTATTCACACTTAT+TGG 0.154119 5_4:+6500542 Msa0833340:three_prime_UTR
ATCCAAACTCTCTTTGTCTT+TGG 0.236659 5_4:+6499820 Msa0833340:five_prime_UTR
TCTCTTTGTCTTTGGCTTCA+TGG 0.240774 5_4:+6499828 Msa0833340:exon
ACATCAAAACCTCACTGTTT+AGG 0.276948 5_4:+6499565 Msa0833340:five_prime_UTR
AATCACTTTAGCATTAATTA+TGG 0.277764 5_4:-6500572 None:intergenic
CTCTTTGTCTTTGGCTTCAT+GGG 0.284185 5_4:+6499829 Msa0833340:exon
CAGGTCACTGCTGGAAAATT+CGG 0.287630 5_4:+6499609 Msa0833340:five_prime_UTR
AATGAGTGAAGGAAGCTTCT+AGG 0.300519 5_4:-6499696 None:intergenic
CATCAAAACCTCACTGTTTA+GGG 0.313886 5_4:+6499566 Msa0833340:five_prime_UTR
AGCCAAAGACAAAGAGAGTT+TGG 0.330320 5_4:-6499822 None:intergenic
TATCCTTTACTTGTTGCATA+AGG 0.330908 5_4:-6500860 None:intergenic
AAACCAATGACTCTCCTCTT+AGG 0.375382 5_4:+6499902 Msa0833340:CDS
ATGTATCTTGTGAAAGGTTA+AGG 0.390025 5_4:+6500654 Msa0833340:three_prime_UTR
TGAAGATACAGTTGGCGAAT+TGG 0.391217 5_4:+6499993 Msa0833340:CDS
ATTATTTGTCAGGTCACTGC+TGG 0.415487 5_4:+6499600 Msa0833340:five_prime_UTR
ATCCTTTACTTGTTGCATAA+GGG 0.417031 5_4:-6500859 None:intergenic
AACGGAAATTTGTCAGTTTG+AGG 0.425120 5_4:-6499868 None:intergenic
TAGTGAGAAATTGAAGAACT+TGG 0.435583 5_4:+6500429 Msa0833340:CDS
TGAGTAGTATGAGGTAGTTT+AGG 0.435990 5_4:+6500814 Msa0833340:three_prime_UTR
TTGAAATGGTGCAGGTTTGA+AGG 0.437401 5_4:+6500405 Msa0833340:intron
TAATTACTTTCAAGCGTGAA+TGG 0.437696 5_4:-6500105 None:intergenic
TGATGAGGCTATTATTTGTC+AGG 0.438001 5_4:+6499590 Msa0833340:five_prime_UTR
AGAGTTTGGATTTCAGTTTA+CGG 0.441806 5_4:-6499808 None:intergenic
AAGTGAAGTTGGTAGCGGTT+TGG 0.461676 5_4:-6500078 None:intergenic
AGGGTAACTAAATATAATTT+GGG 0.462205 5_4:-6500840 None:intergenic
GTGACTGATTGAATCTCAAT+TGG 0.462433 5_4:+6500601 Msa0833340:three_prime_UTR
TGGCCTAAGAGGAGAGTCAT+TGG 0.468313 5_4:-6499905 None:intergenic
TACCCTTATGCAACAAGTAA+AGG 0.468473 5_4:+6500857 Msa0833340:three_prime_UTR
GAAGAGAAGACCACTATTGA+AGG 0.474186 5_4:+6500047 Msa0833340:CDS
TGAACTTCCAAGGCAGCATA+TGG 0.474963 5_4:+6500493 Msa0833340:exon
AGTGAGAAATTGAAGAACTT+GGG 0.475992 5_4:+6500430 Msa0833340:CDS
AAGGGTGAAATTTGAGTTTG+TGG 0.476387 5_4:-6499726 None:intergenic
TTGGAAGTTCAGATTGAAGA+AGG 0.480987 5_4:-6500481 None:intergenic
TGAATGGAGAGAAGTGAAGT+TGG 0.482669 5_4:-6500089 None:intergenic
AGGTCACTGCTGGAAAATTC+GGG 0.491361 5_4:+6499610 Msa0833340:five_prime_UTR
ATATTTCGATTTGTAGAGGT+TGG 0.493266 5_4:-6499654 None:intergenic
AGGGTGAAATTTGAGTTTGT+GGG 0.494506 5_4:-6499725 None:intergenic
AATTGAAGAACTTGGGGTCA+AGG 0.496307 5_4:+6500437 Msa0833340:CDS
AGAAGTTTCGATGGCCTAAG+AGG 0.500163 5_4:-6499916 None:intergenic
TCAATTGCCATATGCTGCCT+TGG 0.502902 5_4:-6500500 None:intergenic
TGTTGTTGTTGTTACGAGAA+AGG 0.511030 5_4:-6499768 None:intergenic
AAATTTATGAATCGTCAAGC+TGG 0.516087 5_4:+6500782 Msa0833340:three_prime_UTR
AATCTTATGTATCTTGTGAA+AGG 0.518070 5_4:+6500648 Msa0833340:three_prime_UTR
GAGAGAAGTGAAGTTGGTAG+CGG 0.523441 5_4:-6500083 None:intergenic
CGAAGCTATTGAGTAGTATG+AGG 0.524961 5_4:+6500805 Msa0833340:three_prime_UTR
TTTGTCTTTGGCTTCATGGG+TGG 0.526303 5_4:+6499832 Msa0833340:exon
TCAAACTGACAAATTTCCGT+TGG 0.528724 5_4:+6499870 Msa0833340:CDS
GTCAGTTTGAGGAGTAGAAA+CGG 0.529246 5_4:-6499857 None:intergenic
GTGTCCATCGAGAACACGTT+GGG 0.537604 5_4:+6499946 Msa0833340:CDS
AATTAATGCTAAAGTGATTG+AGG 0.538671 5_4:+6500576 Msa0833340:three_prime_UTR
TTCTTCAATCTGAACTTCCA+AGG 0.547927 5_4:+6500483 Msa0833340:exon
GGATCGGTGTCCTTCAATAG+TGG 0.549683 5_4:-6500057 None:intergenic
CAAATTTCCGTTGGATACAA+CGG 0.549881 5_4:+6499879 Msa0833340:CDS
TTGGTTTCCGTTGTATCCAA+CGG 0.551596 5_4:-6499886 None:intergenic
AATTCGCCAACTGTATCTTC+AGG 0.565255 5_4:-6499991 None:intergenic
GGGTGAAATTTGAGTTTGTG+GGG 0.570497 5_4:-6499724 None:intergenic
TACAGTTGGCGAATTGGTGA+AGG 0.575497 5_4:+6499999 Msa0833340:CDS
ACTTATATTTCGATTTGTAG+AGG 0.579774 5_4:-6499658 None:intergenic
GTGAGAAATTGAAGAACTTG+GGG 0.585031 5_4:+6500431 Msa0833340:CDS
AAGTTGGTAGCGGTTTGGAT+CGG 0.590772 5_4:-6500073 None:intergenic
CTCACTGTTTAGGGATGATG+AGG 0.592643 5_4:+6499575 Msa0833340:five_prime_UTR
TGCACCCAACGTGTTCTCGA+TGG 0.600223 5_4:-6499950 None:intergenic
TGTCCCTCAAAAGTGTTGAG+TGG 0.609379 5_4:-6500624 None:intergenic
ATGTCGCCTGAAGATACAGT+TGG 0.613993 5_4:+6499985 Msa0833340:CDS
GGTGAAATTTGAGTTTGTGG+GGG 0.618265 5_4:-6499723 None:intergenic
ACGTTGAGTAGAAGTTTCGA+TGG 0.630336 5_4:-6499925 None:intergenic
TGTTGTTGTTACGAGAAAGG+TGG 0.636326 5_4:-6499765 None:intergenic
CGTGTCCATCGAGAACACGT+TGG 0.692755 5_4:+6499945 Msa0833340:CDS
CTCATCATCCCTAAACAGTG+AGG 0.694635 5_4:-6499574 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATAAATTATAAAAAAAAA+GGG - chr5_4:6500155-6500174 None:intergenic 0.0%
!! ATATAAATTATAAAAAAAAA+GGG - chr5_4:6500155-6500174 None:intergenic 0.0%
!! AAGTAAAAAATAATACTGTT+TGG + chr5_4:6500691-6500710 Msa0833340:three_prime_UTR 15.0%
!! AAGTAAAAAATAATACTGTT+TGG + chr5_4:6500691-6500710 Msa0833340:three_prime_UTR 15.0%
!! AAAATGACATGAAAAAAACA+AGG - chr5_4:6500178-6500197 None:intergenic 20.0%
!! TGTATCATATACATATGATA+GGG - chr5_4:6500303-6500322 None:intergenic 20.0%
!! TTGTATCATATACATATGAT+AGG - chr5_4:6500304-6500323 None:intergenic 20.0%
!! AAGATATTATTCACACTTAT+TGG + chr5_4:6500542-6500561 Msa0833340:three_prime_UTR 20.0%
!! AATCACTTTAGCATTAATTA+TGG - chr5_4:6500575-6500594 None:intergenic 20.0%
!! AGGGTAACTAAATATAATTT+GGG - chr5_4:6500843-6500862 None:intergenic 20.0%
!! AAGGGTAACTAAATATAATT+TGG - chr5_4:6500844-6500863 None:intergenic 20.0%
!! AAAATGACATGAAAAAAACA+AGG - chr5_4:6500178-6500197 None:intergenic 20.0%
!! TGTATCATATACATATGATA+GGG - chr5_4:6500303-6500322 None:intergenic 20.0%
!! TTGTATCATATACATATGAT+AGG - chr5_4:6500304-6500323 None:intergenic 20.0%
!! AAGATATTATTCACACTTAT+TGG + chr5_4:6500542-6500561 Msa0833340:three_prime_UTR 20.0%
!! AATCACTTTAGCATTAATTA+TGG - chr5_4:6500575-6500594 None:intergenic 20.0%
!! AGGGTAACTAAATATAATTT+GGG - chr5_4:6500843-6500862 None:intergenic 20.0%
!! AAGGGTAACTAAATATAATT+TGG - chr5_4:6500844-6500863 None:intergenic 20.0%
!! ACTTATATTTCGATTTGTAG+AGG - chr5_4:6499661-6499680 None:intergenic 25.0%
!!! GGTTTTTTTCAAAAGTGAAA+GGG - chr5_4:6499747-6499766 None:intergenic 25.0%
!!! TGGTTTTTTTCAAAAGTGAA+AGG - chr5_4:6499748-6499767 None:intergenic 25.0%
! AAAAAAAAGGGAACATTGAA+GGG - chr5_4:6500143-6500162 None:intergenic 25.0%
! AAAAAAAAAGGGAACATTGA+AGG - chr5_4:6500144-6500163 None:intergenic 25.0%
! AATTAATGCTAAAGTGATTG+AGG + chr5_4:6500576-6500595 Msa0833340:three_prime_UTR 25.0%
! AATCTTATGTATCTTGTGAA+AGG + chr5_4:6500648-6500667 Msa0833340:three_prime_UTR 25.0%
!! ACTTATATTTCGATTTGTAG+AGG - chr5_4:6499661-6499680 None:intergenic 25.0%
!!! GGTTTTTTTCAAAAGTGAAA+GGG - chr5_4:6499747-6499766 None:intergenic 25.0%
!!! TGGTTTTTTTCAAAAGTGAA+AGG - chr5_4:6499748-6499767 None:intergenic 25.0%
! AAAAAAAAGGGAACATTGAA+GGG - chr5_4:6500143-6500162 None:intergenic 25.0%
! AAAAAAAAAGGGAACATTGA+AGG - chr5_4:6500144-6500163 None:intergenic 25.0%
! AATTAATGCTAAAGTGATTG+AGG + chr5_4:6500576-6500595 Msa0833340:three_prime_UTR 25.0%
! AATCTTATGTATCTTGTGAA+AGG + chr5_4:6500648-6500667 Msa0833340:three_prime_UTR 25.0%
! ATATTTCGATTTGTAGAGGT+TGG - chr5_4:6499657-6499676 None:intergenic 30.0%
AGAGTTTGGATTTCAGTTTA+CGG - chr5_4:6499811-6499830 None:intergenic 30.0%
TAATTACTTTCAAGCGTGAA+TGG - chr5_4:6500108-6500127 None:intergenic 30.0%
TGAAACACAAGCATTTCATT+TGG - chr5_4:6500352-6500371 None:intergenic 30.0%
TCGTGAGATAGTTATTGAAA+TGG + chr5_4:6500391-6500410 Msa0833340:intron 30.0%
TAGTGAGAAATTGAAGAACT+TGG + chr5_4:6500429-6500448 Msa0833340:CDS 30.0%
AGTGAGAAATTGAAGAACTT+GGG + chr5_4:6500430-6500449 Msa0833340:CDS 30.0%
ATGTATCTTGTGAAAGGTTA+AGG + chr5_4:6500654-6500673 Msa0833340:three_prime_UTR 30.0%
! AATCTAGCTGCATGTATTTT+TGG + chr5_4:6500723-6500742 Msa0833340:three_prime_UTR 30.0%
AAATTTATGAATCGTCAAGC+TGG + chr5_4:6500782-6500801 Msa0833340:three_prime_UTR 30.0%
! ATCCTTTACTTGTTGCATAA+GGG - chr5_4:6500862-6500881 None:intergenic 30.0%
! TATCCTTTACTTGTTGCATA+AGG - chr5_4:6500863-6500882 None:intergenic 30.0%
! ATATTTCGATTTGTAGAGGT+TGG - chr5_4:6499657-6499676 None:intergenic 30.0%
AGAGTTTGGATTTCAGTTTA+CGG - chr5_4:6499811-6499830 None:intergenic 30.0%
TAATTACTTTCAAGCGTGAA+TGG - chr5_4:6500108-6500127 None:intergenic 30.0%
TGAAACACAAGCATTTCATT+TGG - chr5_4:6500352-6500371 None:intergenic 30.0%
TCGTGAGATAGTTATTGAAA+TGG + chr5_4:6500391-6500410 Msa0833340:intron 30.0%
TAGTGAGAAATTGAAGAACT+TGG + chr5_4:6500429-6500448 Msa0833340:CDS 30.0%
AGTGAGAAATTGAAGAACTT+GGG + chr5_4:6500430-6500449 Msa0833340:CDS 30.0%
ATGTATCTTGTGAAAGGTTA+AGG + chr5_4:6500654-6500673 Msa0833340:three_prime_UTR 30.0%
! AATCTAGCTGCATGTATTTT+TGG + chr5_4:6500723-6500742 Msa0833340:three_prime_UTR 30.0%
AAATTTATGAATCGTCAAGC+TGG + chr5_4:6500782-6500801 Msa0833340:three_prime_UTR 30.0%
! ATCCTTTACTTGTTGCATAA+GGG - chr5_4:6500862-6500881 None:intergenic 30.0%
! TATCCTTTACTTGTTGCATA+AGG - chr5_4:6500863-6500882 None:intergenic 30.0%
!! AGGTTTTGATGTTCATGGAT+TGG - chr5_4:6499557-6499576 None:intergenic 35.0%
ACATCAAAACCTCACTGTTT+AGG + chr5_4:6499565-6499584 Msa0833340:five_prime_UTR 35.0%
CATCAAAACCTCACTGTTTA+GGG + chr5_4:6499566-6499585 Msa0833340:five_prime_UTR 35.0%
TGATGAGGCTATTATTTGTC+AGG + chr5_4:6499590-6499609 Msa0833340:five_prime_UTR 35.0%
! AGGGTGAAATTTGAGTTTGT+GGG - chr5_4:6499728-6499747 None:intergenic 35.0%
! AAGGGTGAAATTTGAGTTTG+TGG - chr5_4:6499729-6499748 None:intergenic 35.0%
! TGTTGTTGTTGTTACGAGAA+AGG - chr5_4:6499771-6499790 None:intergenic 35.0%
ATCCAAACTCTCTTTGTCTT+TGG + chr5_4:6499820-6499839 Msa0833340:five_prime_UTR 35.0%
AACGGAAATTTGTCAGTTTG+AGG - chr5_4:6499871-6499890 None:intergenic 35.0%
TCAAACTGACAAATTTCCGT+TGG + chr5_4:6499870-6499889 Msa0833340:CDS 35.0%
CAAATTTCCGTTGGATACAA+CGG + chr5_4:6499879-6499898 Msa0833340:CDS 35.0%
AACACAAGCATTTCATTTGG+TGG - chr5_4:6500349-6500368 None:intergenic 35.0%
GATAGTTATTGAAATGGTGC+AGG + chr5_4:6500397-6500416 Msa0833340:intron 35.0%
GTGAGAAATTGAAGAACTTG+GGG + chr5_4:6500431-6500450 Msa0833340:CDS 35.0%
TTGGAAGTTCAGATTGAAGA+AGG - chr5_4:6500484-6500503 None:intergenic 35.0%
TTCTTCAATCTGAACTTCCA+AGG + chr5_4:6500483-6500502 Msa0833340:exon 35.0%
GTGACTGATTGAATCTCAAT+TGG + chr5_4:6500601-6500620 Msa0833340:three_prime_UTR 35.0%
TGAGTAGTATGAGGTAGTTT+AGG + chr5_4:6500814-6500833 Msa0833340:three_prime_UTR 35.0%
TACCCTTATGCAACAAGTAA+AGG + chr5_4:6500857-6500876 Msa0833340:three_prime_UTR 35.0%
!! GTTCATGGATTGGTATCTTT+GGG - chr5_4:6499547-6499566 None:intergenic 35.0%
!! TGTTCATGGATTGGTATCTT+TGG - chr5_4:6499548-6499567 None:intergenic 35.0%
!! AGGTTTTGATGTTCATGGAT+TGG - chr5_4:6499557-6499576 None:intergenic 35.0%
ACATCAAAACCTCACTGTTT+AGG + chr5_4:6499565-6499584 Msa0833340:five_prime_UTR 35.0%
CATCAAAACCTCACTGTTTA+GGG + chr5_4:6499566-6499585 Msa0833340:five_prime_UTR 35.0%
TGATGAGGCTATTATTTGTC+AGG + chr5_4:6499590-6499609 Msa0833340:five_prime_UTR 35.0%
! AGGGTGAAATTTGAGTTTGT+GGG - chr5_4:6499728-6499747 None:intergenic 35.0%
! AAGGGTGAAATTTGAGTTTG+TGG - chr5_4:6499729-6499748 None:intergenic 35.0%
! TGTTGTTGTTGTTACGAGAA+AGG - chr5_4:6499771-6499790 None:intergenic 35.0%
ATCCAAACTCTCTTTGTCTT+TGG + chr5_4:6499820-6499839 Msa0833340:five_prime_UTR 35.0%
AACGGAAATTTGTCAGTTTG+AGG - chr5_4:6499871-6499890 None:intergenic 35.0%
TCAAACTGACAAATTTCCGT+TGG + chr5_4:6499870-6499889 Msa0833340:CDS 35.0%
CAAATTTCCGTTGGATACAA+CGG + chr5_4:6499879-6499898 Msa0833340:CDS 35.0%
AACACAAGCATTTCATTTGG+TGG - chr5_4:6500349-6500368 None:intergenic 35.0%
GATAGTTATTGAAATGGTGC+AGG + chr5_4:6500397-6500416 Msa0833340:intron 35.0%
GTGAGAAATTGAAGAACTTG+GGG + chr5_4:6500431-6500450 Msa0833340:CDS 35.0%
TTGGAAGTTCAGATTGAAGA+AGG - chr5_4:6500484-6500503 None:intergenic 35.0%
TTCTTCAATCTGAACTTCCA+AGG + chr5_4:6500483-6500502 Msa0833340:exon 35.0%
GTGACTGATTGAATCTCAAT+TGG + chr5_4:6500601-6500620 Msa0833340:three_prime_UTR 35.0%
TGAGTAGTATGAGGTAGTTT+AGG + chr5_4:6500814-6500833 Msa0833340:three_prime_UTR 35.0%
TACCCTTATGCAACAAGTAA+AGG + chr5_4:6500857-6500876 Msa0833340:three_prime_UTR 35.0%
!! CAGTGAGGTTTTGATGTTCA+TGG - chr5_4:6499562-6499581 None:intergenic 40.0%
ATTATTTGTCAGGTCACTGC+TGG + chr5_4:6499600-6499619 Msa0833340:five_prime_UTR 40.0%
AATGAGTGAAGGAAGCTTCT+AGG - chr5_4:6499699-6499718 None:intergenic 40.0%
! GGTGAAATTTGAGTTTGTGG+GGG - chr5_4:6499726-6499745 None:intergenic 40.0%
! GGGTGAAATTTGAGTTTGTG+GGG - chr5_4:6499727-6499746 None:intergenic 40.0%
! TGTTGTTGTTACGAGAAAGG+TGG - chr5_4:6499768-6499787 None:intergenic 40.0%
AGCCAAAGACAAAGAGAGTT+TGG - chr5_4:6499825-6499844 None:intergenic 40.0%
! TCTCTTTGTCTTTGGCTTCA+TGG + chr5_4:6499828-6499847 Msa0833340:exon 40.0%
! CTCTTTGTCTTTGGCTTCAT+GGG + chr5_4:6499829-6499848 Msa0833340:exon 40.0%
GTCAGTTTGAGGAGTAGAAA+CGG - chr5_4:6499860-6499879 None:intergenic 40.0%
TTGGTTTCCGTTGTATCCAA+CGG - chr5_4:6499889-6499908 None:intergenic 40.0%
AAACCAATGACTCTCCTCTT+AGG + chr5_4:6499902-6499921 Msa0833340:CDS 40.0%
ACGTTGAGTAGAAGTTTCGA+TGG - chr5_4:6499928-6499947 None:intergenic 40.0%
AATTCGCCAACTGTATCTTC+AGG - chr5_4:6499994-6500013 None:intergenic 40.0%
TGAAGATACAGTTGGCGAAT+TGG + chr5_4:6499993-6500012 Msa0833340:CDS 40.0%
GAAGAGAAGACCACTATTGA+AGG + chr5_4:6500047-6500066 Msa0833340:CDS 40.0%
TGAATGGAGAGAAGTGAAGT+TGG - chr5_4:6500092-6500111 None:intergenic 40.0%
AAGGGAACATTGAAGGGTTA+AGG - chr5_4:6500137-6500156 None:intergenic 40.0%
! CATTTGGTGGTTGATTAGCA+TGG - chr5_4:6500336-6500355 None:intergenic 40.0%
TTGAAATGGTGCAGGTTTGA+AGG + chr5_4:6500405-6500424 Msa0833340:intron 40.0%
AATTGAAGAACTTGGGGTCA+AGG + chr5_4:6500437-6500456 Msa0833340:CDS 40.0%
CGAAGCTATTGAGTAGTATG+AGG + chr5_4:6500805-6500824 Msa0833340:three_prime_UTR 40.0%
!! CAGTGAGGTTTTGATGTTCA+TGG - chr5_4:6499562-6499581 None:intergenic 40.0%
ATTATTTGTCAGGTCACTGC+TGG + chr5_4:6499600-6499619 Msa0833340:five_prime_UTR 40.0%
AATGAGTGAAGGAAGCTTCT+AGG - chr5_4:6499699-6499718 None:intergenic 40.0%
! GGTGAAATTTGAGTTTGTGG+GGG - chr5_4:6499726-6499745 None:intergenic 40.0%
! GGGTGAAATTTGAGTTTGTG+GGG - chr5_4:6499727-6499746 None:intergenic 40.0%
! TGTTGTTGTTACGAGAAAGG+TGG - chr5_4:6499768-6499787 None:intergenic 40.0%
AGCCAAAGACAAAGAGAGTT+TGG - chr5_4:6499825-6499844 None:intergenic 40.0%
! TCTCTTTGTCTTTGGCTTCA+TGG + chr5_4:6499828-6499847 Msa0833340:exon 40.0%
! CTCTTTGTCTTTGGCTTCAT+GGG + chr5_4:6499829-6499848 Msa0833340:exon 40.0%
GTCAGTTTGAGGAGTAGAAA+CGG - chr5_4:6499860-6499879 None:intergenic 40.0%
TTGGTTTCCGTTGTATCCAA+CGG - chr5_4:6499889-6499908 None:intergenic 40.0%
AAACCAATGACTCTCCTCTT+AGG + chr5_4:6499902-6499921 Msa0833340:CDS 40.0%
ACGTTGAGTAGAAGTTTCGA+TGG - chr5_4:6499928-6499947 None:intergenic 40.0%
AATTCGCCAACTGTATCTTC+AGG - chr5_4:6499994-6500013 None:intergenic 40.0%
TGAAGATACAGTTGGCGAAT+TGG + chr5_4:6499993-6500012 Msa0833340:CDS 40.0%
GAAGAGAAGACCACTATTGA+AGG + chr5_4:6500047-6500066 Msa0833340:CDS 40.0%
TGAATGGAGAGAAGTGAAGT+TGG - chr5_4:6500092-6500111 None:intergenic 40.0%
AAGGGAACATTGAAGGGTTA+AGG - chr5_4:6500137-6500156 None:intergenic 40.0%
! CATTTGGTGGTTGATTAGCA+TGG - chr5_4:6500336-6500355 None:intergenic 40.0%
TTGAAATGGTGCAGGTTTGA+AGG + chr5_4:6500405-6500424 Msa0833340:intron 40.0%
AATTGAAGAACTTGGGGTCA+AGG + chr5_4:6500437-6500456 Msa0833340:CDS 40.0%
CGAAGCTATTGAGTAGTATG+AGG + chr5_4:6500805-6500824 Msa0833340:three_prime_UTR 40.0%
CTCATCATCCCTAAACAGTG+AGG - chr5_4:6499577-6499596 None:intergenic 45.0%
CTCACTGTTTAGGGATGATG+AGG + chr5_4:6499575-6499594 Msa0833340:five_prime_UTR 45.0%
CAGGTCACTGCTGGAAAATT+CGG + chr5_4:6499609-6499628 Msa0833340:five_prime_UTR 45.0%
AGGTCACTGCTGGAAAATTC+GGG + chr5_4:6499610-6499629 Msa0833340:five_prime_UTR 45.0%
! TTTGTCTTTGGCTTCATGGG+TGG + chr5_4:6499832-6499851 Msa0833340:exon 45.0%
AGAAGTTTCGATGGCCTAAG+AGG - chr5_4:6499919-6499938 None:intergenic 45.0%
ATGTCGCCTGAAGATACAGT+TGG + chr5_4:6499985-6500004 Msa0833340:CDS 45.0%
TACAGTTGGCGAATTGGTGA+AGG + chr5_4:6499999-6500018 Msa0833340:CDS 45.0%
!! AAGTTGGTAGCGGTTTGGAT+CGG - chr5_4:6500076-6500095 None:intergenic 45.0%
!! AAGTGAAGTTGGTAGCGGTT+TGG - chr5_4:6500081-6500100 None:intergenic 45.0%
!! GAGAGAAGTGAAGTTGGTAG+CGG - chr5_4:6500086-6500105 None:intergenic 45.0%
TGAACTTCCAAGGCAGCATA+TGG + chr5_4:6500493-6500512 Msa0833340:exon 45.0%
TCAATTGCCATATGCTGCCT+TGG - chr5_4:6500503-6500522 None:intergenic 45.0%
! TTGGCCACTCAACACTTTTG+AGG + chr5_4:6500620-6500639 Msa0833340:three_prime_UTR 45.0%
! TGGCCACTCAACACTTTTGA+GGG + chr5_4:6500621-6500640 Msa0833340:three_prime_UTR 45.0%
!! TGTCCCTCAAAAGTGTTGAG+TGG - chr5_4:6500627-6500646 None:intergenic 45.0%
CTCATCATCCCTAAACAGTG+AGG - chr5_4:6499577-6499596 None:intergenic 45.0%
CTCACTGTTTAGGGATGATG+AGG + chr5_4:6499575-6499594 Msa0833340:five_prime_UTR 45.0%
CAGGTCACTGCTGGAAAATT+CGG + chr5_4:6499609-6499628 Msa0833340:five_prime_UTR 45.0%
AGGTCACTGCTGGAAAATTC+GGG + chr5_4:6499610-6499629 Msa0833340:five_prime_UTR 45.0%
! TTTGTCTTTGGCTTCATGGG+TGG + chr5_4:6499832-6499851 Msa0833340:exon 45.0%
AGAAGTTTCGATGGCCTAAG+AGG - chr5_4:6499919-6499938 None:intergenic 45.0%
ATGTCGCCTGAAGATACAGT+TGG + chr5_4:6499985-6500004 Msa0833340:CDS 45.0%
TACAGTTGGCGAATTGGTGA+AGG + chr5_4:6499999-6500018 Msa0833340:CDS 45.0%
!! AAGTTGGTAGCGGTTTGGAT+CGG - chr5_4:6500076-6500095 None:intergenic 45.0%
!! AAGTGAAGTTGGTAGCGGTT+TGG - chr5_4:6500081-6500100 None:intergenic 45.0%
!! GAGAGAAGTGAAGTTGGTAG+CGG - chr5_4:6500086-6500105 None:intergenic 45.0%
TGAACTTCCAAGGCAGCATA+TGG + chr5_4:6500493-6500512 Msa0833340:exon 45.0%
TCAATTGCCATATGCTGCCT+TGG - chr5_4:6500503-6500522 None:intergenic 45.0%
! TTGGCCACTCAACACTTTTG+AGG + chr5_4:6500620-6500639 Msa0833340:three_prime_UTR 45.0%
! TGGCCACTCAACACTTTTGA+GGG + chr5_4:6500621-6500640 Msa0833340:three_prime_UTR 45.0%
!! TGTCCCTCAAAAGTGTTGAG+TGG - chr5_4:6500627-6500646 None:intergenic 45.0%
!! GATATAAATTATAAAAAAAA+AGG - chr5_4:6500156-6500175 None:intergenic 5.0%
!! GATATAAATTATAAAAAAAA+AGG - chr5_4:6500156-6500175 None:intergenic 5.0%
GTGGGGGCTTAAATGAGTGA+AGG - chr5_4:6499710-6499729 None:intergenic 50.0%
TGGCCTAAGAGGAGAGTCAT+TGG - chr5_4:6499908-6499927 None:intergenic 50.0%
GTGTCCATCGAGAACACGTT+GGG + chr5_4:6499946-6499965 Msa0833340:CDS 50.0%
! GGATCGGTGTCCTTCAATAG+TGG - chr5_4:6500060-6500079 None:intergenic 50.0%
GTGGGGGCTTAAATGAGTGA+AGG - chr5_4:6499710-6499729 None:intergenic 50.0%
TGGCCTAAGAGGAGAGTCAT+TGG - chr5_4:6499908-6499927 None:intergenic 50.0%
GTGTCCATCGAGAACACGTT+GGG + chr5_4:6499946-6499965 Msa0833340:CDS 50.0%
! GGATCGGTGTCCTTCAATAG+TGG - chr5_4:6500060-6500079 None:intergenic 50.0%
CGTGTCCATCGAGAACACGT+TGG + chr5_4:6499945-6499964 Msa0833340:CDS 55.0%
TGCACCCAACGTGTTCTCGA+TGG - chr5_4:6499953-6499972 None:intergenic 55.0%
CGTGTCCATCGAGAACACGT+TGG + chr5_4:6499945-6499964 Msa0833340:CDS 55.0%
TGCACCCAACGTGTTCTCGA+TGG - chr5_4:6499953-6499972 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 6499547 6500894 6499547 ID=Msa0833340;Name=Msa0833340
chr5_4 mRNA 6499547 6500894 6499547 ID=Msa0833340-mRNA-1;Parent=Msa0833340;Name=Msa0833340-mRNA-1;_AED=0.02;_eAED=0.02;_QI=300|1|1|1|1|1|2|399|116
chr5_4 exon 6499547 6500120 6499547 ID=Msa0833340-mRNA-1:exon:2836;Parent=Msa0833340-mRNA-1
chr5_4 exon 6500419 6500894 6500419 ID=Msa0833340-mRNA-1:exon:2837;Parent=Msa0833340-mRNA-1
chr5_4 five_prime_UTR 6499547 6499846 6499547 ID=Msa0833340-mRNA-1:five_prime_utr;Parent=Msa0833340-mRNA-1
chr5_4 CDS 6499847 6500120 6499847 ID=Msa0833340-mRNA-1:cds;Parent=Msa0833340-mRNA-1
chr5_4 CDS 6500419 6500495 6500419 ID=Msa0833340-mRNA-1:cds;Parent=Msa0833340-mRNA-1
chr5_4 three_prime_UTR 6500496 6500894 6500496 ID=Msa0833340-mRNA-1:three_prime_utr;Parent=Msa0833340-mRNA-1
Gene Sequence

>Msa0833340

ATGGGTGGTACCGTTTCTACTCCTCAAACTGACAAATTTCCGTTGGATACAACGGAAACCAATGACTCTCCTCTTAGGCCATCGAAACTTCTACTCAACGTGTCCATCGAGAACACGTTGGGTGCAATACAAGTTTTGATGTCGCCTGAAGATACAGTTGGCGAATTGGTGAAGGTAGCATTGCTCACTTACGATAAAGAGAAGAGAAGACCACTATTGAAGGACACCGATCCAAACCGCTACCAACTTCACTTCTCTCCATTCACGCTTGAAAGTTTGAAGGTTAGTGAGAAATTGAAGAACTTGGGGTCAAGGAATTTCTTTCTATGCTCAAAGCCTTCTTCAATCTGA
Protein sequence

>Msa0833340

MGGTVSTPQTDKFPLDTTETNDSPLRPSKLLLNVSIENTLGAIQVLMSPEDTVGELVKVALLTYDKEKRRPLLKDTDPNRYQLHFSPFTLESLKVSEKLKNLGSRNFFLCSKPSSI*