AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0534570


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0534570 MtrunA17_Chr4g0008511 92.727 55 3 1 28 81 446 500 1.59e-26 100
Msa0534570 MtrunA17_Chr4g0008491 66.667 51 17 0 28 78 260 310 4.80e-18 75.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 22 sgRNAs with CRISPR-Local

Find 104 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCTTCCTTTGGATTCTTA+AGG 0.299759 4_1:+10075057 None:intergenic
ACCGTCTGTCAAAGCCTCTT+TGG 0.309921 4_1:+10075091 None:intergenic
CAAGCTGTAACTTCTTCCTT+TGG 0.353157 4_1:+10075047 None:intergenic
GAATGTACCATTGGATGATT+TGG 0.353597 4_1:-10075557 Msa0534570:CDS
AGAGATGTTGGATAAGGAAA+TGG 0.479902 4_1:-10076074 None:intergenic
AAGTCAAAGAAGCTCCCAAC+AGG 0.484404 4_1:+10075117 None:intergenic
TTCTTTGACTTCTTCCAAAG+AGG 0.487396 4_1:-10075105 Msa0534570:CDS
TGCAAAAGAGATGTTGGATA+AGG 0.487646 4_1:-10076080 None:intergenic
TGTCAGGCACTGAACCTGTT+GGG 0.511727 4_1:-10075131 Msa0534570:intron
TCCAAAGAGGCTTTGACAGA+CGG 0.518614 4_1:-10075092 Msa0534570:CDS
GAGGAGCATATAGTGAGCAT+TGG 0.518756 4_1:-10075535 Msa0534570:CDS
CTTCCTTTGGATTCTTAAGG+AGG 0.541667 4_1:+10075060 None:intergenic
TGGAAAATCAGAGTGAAGAG+AGG 0.542968 4_1:-10076051 Msa0534570:CDS
GCACAAGATGAATGTACCAT+TGG 0.543115 4_1:-10075566 Msa0534570:intron
TGTACCATTGGATGATTTGG+AGG 0.570776 4_1:-10075554 Msa0534570:CDS
GATGTTGGATAAGGAAATGG+TGG 0.572311 4_1:-10076071 Msa0534570:CDS
TTGTCAGGCACTGAACCTGT+TGG 0.605048 4_1:-10075132 Msa0534570:intron
TGCTCCTCCAAATCATCCAA+TGG 0.607505 4_1:+10075550 None:intergenic
CTACCTCCTTAAGAATCCAA+AGG 0.611859 4_1:-10075063 Msa0534570:CDS
GATTGATGAGAGAGAACGAA+GGG 0.631467 4_1:-10076005 Msa0534570:intron
AGTGAGCATTGGAGAAACAG+AGG 0.672512 4_1:-10075524 Msa0534570:intron
TGATTGATGAGAGAGAACGA+AGG 0.727933 4_1:-10076006 Msa0534570:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAATAAAATTATTTTTTAA+TGG - chr4_1:10075406-10075425 Msa0534570:intron 0.0%
!! TATATATATATATATATATA+TGG - chr4_1:10075827-10075846 Msa0534570:intron 0.0%
!!! AAAATAAAATTATTTTTTAA+TGG - chr4_1:10075406-10075425 Msa0534570:intron 0.0%
!! TATATATATATATATATATA+TGG - chr4_1:10075827-10075846 Msa0534570:intron 0.0%
!! AATCTTAATAATATACAAAT+GGG + chr4_1:10075503-10075522 None:intergenic 10.0%
!!! TTTTCTTGTTAATTTTTTTT+GGG + chr4_1:10075765-10075784 None:intergenic 10.0%
!!! ATTTTCTTGTTAATTTTTTT+TGG + chr4_1:10075766-10075785 None:intergenic 10.0%
!! AATCTTAATAATATACAAAT+GGG + chr4_1:10075503-10075522 None:intergenic 10.0%
!!! TTTTCTTGTTAATTTTTTTT+GGG + chr4_1:10075765-10075784 None:intergenic 10.0%
!!! ATTTTCTTGTTAATTTTTTT+TGG + chr4_1:10075766-10075785 None:intergenic 10.0%
!! ATCTTAATAATATACAAATG+GGG + chr4_1:10075502-10075521 None:intergenic 15.0%
!! GAATCTTAATAATATACAAA+TGG + chr4_1:10075504-10075523 None:intergenic 15.0%
!!! ATTTTTTTTTCTTCCTTAAT+CGG - chr4_1:10075713-10075732 Msa0534570:intron 15.0%
!! TAACAAGAAAATAGATATTA+AGG - chr4_1:10075774-10075793 Msa0534570:intron 15.0%
!! CATTGATATATTATAAAACT+AGG - chr4_1:10075899-10075918 Msa0534570:intron 15.0%
!! ATCTTAATAATATACAAATG+GGG + chr4_1:10075502-10075521 None:intergenic 15.0%
!! GAATCTTAATAATATACAAA+TGG + chr4_1:10075504-10075523 None:intergenic 15.0%
!!! ATTTTTTTTTCTTCCTTAAT+CGG - chr4_1:10075713-10075732 Msa0534570:intron 15.0%
!! TAACAAGAAAATAGATATTA+AGG - chr4_1:10075774-10075793 Msa0534570:intron 15.0%
!! CATTGATATATTATAAAACT+AGG - chr4_1:10075899-10075918 Msa0534570:intron 15.0%
!! GAAATAAAATGTTAATAGTG+AGG - chr4_1:10075185-10075204 Msa0534570:intron 20.0%
!! TTGAAATGAATAATAGAGAA+AGG - chr4_1:10075225-10075244 Msa0534570:intron 20.0%
!!! ATTTGAATTTTGAATTGAAG+AGG - chr4_1:10075326-10075345 Msa0534570:intron 20.0%
!!! TTTGAATTTTGAATTGAAGA+GGG - chr4_1:10075327-10075346 Msa0534570:intron 20.0%
!! TGATATATTATAAAACTAGG+TGG - chr4_1:10075902-10075921 Msa0534570:intron 20.0%
!! GAAATAAAATGTTAATAGTG+AGG - chr4_1:10075185-10075204 Msa0534570:intron 20.0%
!! TTGAAATGAATAATAGAGAA+AGG - chr4_1:10075225-10075244 Msa0534570:intron 20.0%
!!! ATTTGAATTTTGAATTGAAG+AGG - chr4_1:10075326-10075345 Msa0534570:intron 20.0%
!!! TTTGAATTTTGAATTGAAGA+GGG - chr4_1:10075327-10075346 Msa0534570:intron 20.0%
!! TGATATATTATAAAACTAGG+TGG - chr4_1:10075902-10075921 Msa0534570:intron 20.0%
! AAAGAGAGAAACGAAATAAA+AGG - chr4_1:10075147-10075166 Msa0534570:intron 25.0%
! TGTTAATAGTGAGGAAAAAA+AGG - chr4_1:10075194-10075213 Msa0534570:intron 25.0%
! AAATTGAAAGTGATGTTTGT+TGG - chr4_1:10075256-10075275 Msa0534570:intron 25.0%
! CTTGATACTTGGATATTTAA+TGG - chr4_1:10075663-10075682 Msa0534570:intron 25.0%
! TTAATTATCTCTACATTGTC+AGG - chr4_1:10075969-10075988 Msa0534570:intron 25.0%
! AAAGAGAGAAACGAAATAAA+AGG - chr4_1:10075147-10075166 Msa0534570:intron 25.0%
! TGTTAATAGTGAGGAAAAAA+AGG - chr4_1:10075194-10075213 Msa0534570:intron 25.0%
! AAATTGAAAGTGATGTTTGT+TGG - chr4_1:10075256-10075275 Msa0534570:intron 25.0%
! CTTGATACTTGGATATTTAA+TGG - chr4_1:10075663-10075682 Msa0534570:intron 25.0%
! TTAATTATCTCTACATTGTC+AGG - chr4_1:10075969-10075988 Msa0534570:intron 25.0%
GTTGGTATAAGAGAAAAAGT+AGG - chr4_1:10075274-10075293 Msa0534570:intron 30.0%
GTATAAGAGAAAAAGTAGGA+GGG - chr4_1:10075278-10075297 Msa0534570:intron 30.0%
TATTGTGTGAATGCTTAAAG+TGG - chr4_1:10075381-10075400 Msa0534570:intron 30.0%
!! TGCTTAGAATTTTGACACAT+TGG - chr4_1:10075445-10075464 Msa0534570:intron 30.0%
! TCACTTGCTTCTAGTTATAA+AGG + chr4_1:10075944-10075963 Msa0534580:five_prime_UTR 30.0%
GTTGGTATAAGAGAAAAAGT+AGG - chr4_1:10075274-10075293 Msa0534570:intron 30.0%
GTATAAGAGAAAAAGTAGGA+GGG - chr4_1:10075278-10075297 Msa0534570:intron 30.0%
TATTGTGTGAATGCTTAAAG+TGG - chr4_1:10075381-10075400 Msa0534570:intron 30.0%
!! TGCTTAGAATTTTGACACAT+TGG - chr4_1:10075445-10075464 Msa0534570:intron 30.0%
! TCACTTGCTTCTAGTTATAA+AGG + chr4_1:10075944-10075963 Msa0534580:five_prime_UTR 30.0%
AACGAAATAAAAGGAGTGAG+AGG - chr4_1:10075156-10075175 Msa0534570:intron 35.0%
GGTATAAGAGAAAAAGTAGG+AGG - chr4_1:10075277-10075296 Msa0534570:intron 35.0%
GAATGTACCATTGGATGATT+TGG - chr4_1:10075559-10075578 Msa0534570:CDS 35.0%
TCCTTGCAAATCTTGATACT+TGG - chr4_1:10075652-10075671 Msa0534570:intron 35.0%
TCCAAGTATCAAGATTTGCA+AGG + chr4_1:10075656-10075675 None:intergenic 35.0%
TTAAGCTAACTAGTGTCTCA+GGG + chr4_1:10075742-10075761 None:intergenic 35.0%
! TTCTTTGACTTCTTCCAAAG+AGG - chr4_1:10076011-10076030 Msa0534570:CDS 35.0%
CTTCTTCCTTTGGATTCTTA+AGG + chr4_1:10076062-10076081 Msa0534580:five_prime_UTR 35.0%
AACGAAATAAAAGGAGTGAG+AGG - chr4_1:10075156-10075175 Msa0534570:intron 35.0%
GGTATAAGAGAAAAAGTAGG+AGG - chr4_1:10075277-10075296 Msa0534570:intron 35.0%
GAATGTACCATTGGATGATT+TGG - chr4_1:10075559-10075578 Msa0534570:CDS 35.0%
TCCTTGCAAATCTTGATACT+TGG - chr4_1:10075652-10075671 Msa0534570:intron 35.0%
TCCAAGTATCAAGATTTGCA+AGG + chr4_1:10075656-10075675 None:intergenic 35.0%
TTAAGCTAACTAGTGTCTCA+GGG + chr4_1:10075742-10075761 None:intergenic 35.0%
! TTCTTTGACTTCTTCCAAAG+AGG - chr4_1:10076011-10076030 Msa0534570:CDS 35.0%
CTTCTTCCTTTGGATTCTTA+AGG + chr4_1:10076062-10076081 Msa0534580:five_prime_UTR 35.0%
TGGAAAATCAGAGTGAAGAG+AGG - chr4_1:10075065-10075084 Msa0534570:CDS 40.0%
TGATTGATGAGAGAGAACGA+AGG - chr4_1:10075110-10075129 Msa0534570:CDS 40.0%
GATTGATGAGAGAGAACGAA+GGG - chr4_1:10075111-10075130 Msa0534570:CDS 40.0%
GAAAAAGTAGGAGGGAAAGA+AGG - chr4_1:10075286-10075305 Msa0534570:intron 40.0%
GCACAAGATGAATGTACCAT+TGG - chr4_1:10075550-10075569 Msa0534570:CDS 40.0%
! TGTACCATTGGATGATTTGG+AGG - chr4_1:10075562-10075581 Msa0534570:CDS 40.0%
GTTAAGCTAACTAGTGTCTC+AGG + chr4_1:10075743-10075762 None:intergenic 40.0%
CTACCTCCTTAAGAATCCAA+AGG - chr4_1:10076053-10076072 Msa0534570:CDS 40.0%
CTTCCTTTGGATTCTTAAGG+AGG + chr4_1:10076059-10076078 Msa0534580:five_prime_UTR 40.0%
CAAGCTGTAACTTCTTCCTT+TGG + chr4_1:10076072-10076091 Msa0534580:five_prime_UTR 40.0%
TGGAAAATCAGAGTGAAGAG+AGG - chr4_1:10075065-10075084 Msa0534570:CDS 40.0%
TGATTGATGAGAGAGAACGA+AGG - chr4_1:10075110-10075129 Msa0534570:CDS 40.0%
GATTGATGAGAGAGAACGAA+GGG - chr4_1:10075111-10075130 Msa0534570:CDS 40.0%
GAAAAAGTAGGAGGGAAAGA+AGG - chr4_1:10075286-10075305 Msa0534570:intron 40.0%
GCACAAGATGAATGTACCAT+TGG - chr4_1:10075550-10075569 Msa0534570:CDS 40.0%
! TGTACCATTGGATGATTTGG+AGG - chr4_1:10075562-10075581 Msa0534570:CDS 40.0%
GTTAAGCTAACTAGTGTCTC+AGG + chr4_1:10075743-10075762 None:intergenic 40.0%
CTACCTCCTTAAGAATCCAA+AGG - chr4_1:10076053-10076072 Msa0534570:CDS 40.0%
CTTCCTTTGGATTCTTAAGG+AGG + chr4_1:10076059-10076078 Msa0534580:five_prime_UTR 40.0%
CAAGCTGTAACTTCTTCCTT+TGG + chr4_1:10076072-10076091 Msa0534580:five_prime_UTR 40.0%
TGCTCCTCCAAATCATCCAA+TGG + chr4_1:10075569-10075588 None:intergenic 45.0%
! GAGGAGCATATAGTGAGCAT+TGG - chr4_1:10075581-10075600 Msa0534570:intron 45.0%
!! AGTGAGCATTGGAGAAACAG+AGG - chr4_1:10075592-10075611 Msa0534570:intron 45.0%
AAGTCAAAGAAGCTCCCAAC+AGG + chr4_1:10076002-10076021 Msa0534580:five_prime_UTR 45.0%
! TCCAAAGAGGCTTTGACAGA+CGG - chr4_1:10076024-10076043 Msa0534570:CDS 45.0%
TGCTCCTCCAAATCATCCAA+TGG + chr4_1:10075569-10075588 None:intergenic 45.0%
! GAGGAGCATATAGTGAGCAT+TGG - chr4_1:10075581-10075600 Msa0534570:intron 45.0%
!! AGTGAGCATTGGAGAAACAG+AGG - chr4_1:10075592-10075611 Msa0534570:intron 45.0%
AAGTCAAAGAAGCTCCCAAC+AGG + chr4_1:10076002-10076021 Msa0534580:five_prime_UTR 45.0%
! TCCAAAGAGGCTTTGACAGA+CGG - chr4_1:10076024-10076043 Msa0534570:CDS 45.0%
! TGTCTCAGGGACACCGATTA+AGG + chr4_1:10075729-10075748 None:intergenic 50.0%
!! TTGTCAGGCACTGAACCTGT+TGG - chr4_1:10075984-10076003 Msa0534570:intron 50.0%
!! TGTCAGGCACTGAACCTGTT+GGG - chr4_1:10075985-10076004 Msa0534570:intron 50.0%
ACCGTCTGTCAAAGCCTCTT+TGG + chr4_1:10076028-10076047 Msa0534580:five_prime_UTR 50.0%
! TGTCTCAGGGACACCGATTA+AGG + chr4_1:10075729-10075748 None:intergenic 50.0%
!! TTGTCAGGCACTGAACCTGT+TGG - chr4_1:10075984-10076003 Msa0534570:intron 50.0%
!! TGTCAGGCACTGAACCTGTT+GGG - chr4_1:10075985-10076004 Msa0534570:intron 50.0%
ACCGTCTGTCAAAGCCTCTT+TGG + chr4_1:10076028-10076047 Msa0534580:five_prime_UTR 50.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 10075046 10076092 10075046 ID=Msa0534570;Name=Msa0534570
chr4_1 mRNA 10075046 10076092 10075046 ID=Msa0534570-mRNA-1;Parent=Msa0534570;Name=Msa0534570-mRNA-1;_AED=0.66;_eAED=0.66;_QI=0|0|0|0.66|1|1|3|0|81
chr4_1 exon 10076006 10076092 10076006 ID=Msa0534570-mRNA-1:exon:3249;Parent=Msa0534570-mRNA-1
chr4_1 exon 10075525 10075581 10075525 ID=Msa0534570-mRNA-1:exon:3248;Parent=Msa0534570-mRNA-1
chr4_1 exon 10075046 10075147 10075046 ID=Msa0534570-mRNA-1:exon:3247;Parent=Msa0534570-mRNA-1
chr4_1 CDS 10076006 10076092 10076006 ID=Msa0534570-mRNA-1:cds;Parent=Msa0534570-mRNA-1
chr4_1 CDS 10075525 10075581 10075525 ID=Msa0534570-mRNA-1:cds;Parent=Msa0534570-mRNA-1
chr4_1 CDS 10075046 10075147 10075046 ID=Msa0534570-mRNA-1:cds;Parent=Msa0534570-mRNA-1
Gene Sequence

>Msa0534570

ATGTTGGATAAGGAAATGGTGGAAAATCAGAGTGAAGAGAGGAAGAAATCTGTCTCTGATTTCTTGATTGATGAGAGAGAACGAAGGATGAATGTACCATTGGATGATTTGGAGGAGCATATAGTGAGCATTGGAGAAACAGAGGCACTGAACCTGTTGGGAGCTTCTTTGACTTCTTCCAAAGAGGCTTTGACAGACGGTTTGTTCTACCTCCTTAAGAATCCAAAGGAAGAAGTTACAGCTTGA

Protein sequence

>Msa0534570

MLDKEMVENQSEERKKSVSDFLIDERERRMNVPLDDLEEHIVSIGETEALNLLGASLTSSKEALTDGLFYLLKNPKEEVTA*