AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0754370

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0754370 MtrunA17_Chr5g0398651 93.966 116 7 0 1 116 1 116 9.08e-77 222
Msa0754370 MtrunA17_Chr4g0056791 62.385 109 36 1 5 113 41 144 6.96e-40 130
Msa0754370 MtrunA17_Chr2g0320881 53.125 96 45 0 20 115 51 146 7.09e-27 98.6
Msa0754370 MtrunA17_Chr4g0027171 50.562 89 44 0 24 112 67 155 6.46e-26 95.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0754370 AT2G27830.1 52.128 94 45 0 19 112 61 154 1.09e-30 108

Find 70 sgRNAs with CRISPR-Local

Find 184 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGGGCAACTAAATATAATT+TGG 0.142333 5_2:-7925389 None:intergenic
AAGATATTATTCACACTTAT+TGG 0.154119 5_2:+7925090 Msa0754370:three_prime_UTR
ATCCAAACTCTCTTTGTCTT+TGG 0.236659 5_2:+7924406 Msa0754370:five_prime_UTR
TCTCTTTGTCTTTGGCTTCA+TGG 0.240774 5_2:+7924414 Msa0754370:exon
ACATCAAAACCTCACTGTTT+AGG 0.276948 5_2:+7924154 Msa0754370:five_prime_UTR
AATCACTTTAGCATTAATTA+TGG 0.277764 5_2:-7925120 None:intergenic
CTCTTTGTCTTTGGCTTCAT+GGG 0.284185 5_2:+7924415 Msa0754370:exon
CAGGTCACTGCTGGAAAATT+CGG 0.287630 5_2:+7924198 Msa0754370:five_prime_UTR
AATGAGTGAAGGAAGCTTCT+AGG 0.300519 5_2:-7924285 None:intergenic
CATCAAAACCTCACTGTTTA+GGG 0.313886 5_2:+7924155 Msa0754370:five_prime_UTR
AGCCAAAGACAAAGAGAGTT+TGG 0.330320 5_2:-7924408 None:intergenic
TATCCTTTACATGTTGCATA+AGG 0.355932 5_2:-7925408 None:intergenic
ATGTATCTTGTGAAAGGTTA+AGG 0.390025 5_2:+7925202 Msa0754370:three_prime_UTR
AGGGCAACTAAATATAATTT+GGG 0.412005 5_2:-7925388 None:intergenic
ATTATTTGTCAGGTCACTGC+TGG 0.415487 5_2:+7924189 Msa0754370:five_prime_UTR
TGCATTGATGACTTATGATA+AGG 0.419305 5_2:+7924609 Msa0754370:CDS
AACGGAAATTTGTCAGTTTG+AGG 0.425120 5_2:-7924454 None:intergenic
TAGTGAGAAATTGAAGAACT+TGG 0.435583 5_2:+7924977 Msa0754370:CDS
TGAGTAGTATGAGGTAGTTT+AGG 0.435990 5_2:+7925362 Msa0754370:three_prime_UTR
TAATTACTTTCAAGCGTGAA+TGG 0.437696 5_2:-7924691 None:intergenic
TGATGAGGCTATTATTTGTC+AGG 0.438001 5_2:+7924179 Msa0754370:five_prime_UTR
AGAGTTTGGATTTCAGTTTA+CGG 0.441806 5_2:-7924394 None:intergenic
ATGAAATGGTGCAGGTTTGA+AGG 0.447179 5_2:+7924953 Msa0754370:intron
ATCCTTTACATGTTGCATAA+GGG 0.449046 5_2:-7925407 None:intergenic
GGATCGGTGTCCTTCAATAA+TGG 0.452970 5_2:-7924643 None:intergenic
GTGACTGATTGAATCTCAAT+TGG 0.460220 5_2:+7925149 Msa0754370:three_prime_UTR
AAGTGAAGTTGGTAGCGGTT+TGG 0.461676 5_2:-7924664 None:intergenic
TGCCCTTATGCAACATGTAA+AGG 0.473630 5_2:+7925405 Msa0754370:three_prime_UTR
TGAACTTCCAAGGCAGCATA+TGG 0.474963 5_2:+7925041 Msa0754370:exon
AGTGAGAAATTGAAGAACTT+GGG 0.475992 5_2:+7924978 Msa0754370:CDS
AAGGGTGAAATTTGAGTTTG+TGG 0.476387 5_2:-7924315 None:intergenic
TGAATGGAGAGAAGTGAAGT+TGG 0.482669 5_2:-7924675 None:intergenic
TTGGAAGTTCAGATTGAAGT+AGG 0.484151 5_2:-7925029 None:intergenic
AATTAATGCTAAAGTGATTG+AGG 0.488321 5_2:+7925124 Msa0754370:three_prime_UTR
GAAGAGAAGGCCATTATTGA+AGG 0.491115 5_2:+7924633 Msa0754370:CDS
AGGTCACTGCTGGAAAATTC+GGG 0.491361 5_2:+7924199 Msa0754370:five_prime_UTR
ATATTTCGATTTGTAGAGGT+TGG 0.493266 5_2:-7924243 None:intergenic
AGGGTGAAATTTGAGTTTGT+GGG 0.494506 5_2:-7924314 None:intergenic
AATTGAAGAACTTGGGGTCA+AGG 0.499762 5_2:+7924985 Msa0754370:CDS
TCAATTGCCATATGCTGCCT+TGG 0.502902 5_2:-7925048 None:intergenic
TGGTCTAAGAGGAGAGTCAT+TGG 0.503838 5_2:-7924491 None:intergenic
TGTTGTTGTTGTTACGAGAA+AGG 0.503927 5_2:-7924357 None:intergenic
AAATTTATGAATCGTCAAGC+TGG 0.516087 5_2:+7925330 Msa0754370:three_prime_UTR
AATCTTATGTATCTTGTGAA+AGG 0.518070 5_2:+7925196 Msa0754370:three_prime_UTR
AGAAGTTTCGATGGTCTAAG+AGG 0.521243 5_2:-7924502 None:intergenic
GAGAGAAGTGAAGTTGGTAG+CGG 0.523441 5_2:-7924669 None:intergenic
CGAAGCTATTGAGTAGTATG+AGG 0.524961 5_2:+7925353 Msa0754370:three_prime_UTR
TTTGTCTTTGGCTTCATGGG+TGG 0.526303 5_2:+7924418 Msa0754370:exon
TCAAACTGACAAATTTCCGT+TGG 0.528724 5_2:+7924456 Msa0754370:CDS
GTCAGTTTGAGGAGTAGAAA+CGG 0.529246 5_2:-7924443 None:intergenic
GTGTCCATCGAGAACACGTT+GGG 0.537604 5_2:+7924532 Msa0754370:CDS
CAAATTTCCGTTGGATACAA+CGG 0.549881 5_2:+7924465 Msa0754370:CDS
TTGGTTTCCGTTGTATCCAA+CGG 0.551596 5_2:-7924472 None:intergenic
TACCGTTGGCGAATTAGTGA+AGG 0.567706 5_2:+7924585 Msa0754370:CDS
GGGTGAAATTTGAGTTTGTG+GGG 0.570497 5_2:-7924313 None:intergenic
ACTTATATTTCGATTTGTAG+AGG 0.579774 5_2:-7924247 None:intergenic
GTGAGAAATTGAAGAACTTG+GGG 0.585031 5_2:+7924979 Msa0754370:CDS
CTTATGATAAGGAGAAGAGA+AGG 0.588206 5_2:+7924620 Msa0754370:CDS
AAGTTGGTAGCGGTTTGGAT+CGG 0.590772 5_2:-7924659 None:intergenic
CTCACTGTTTAGGGATGATG+AGG 0.592643 5_2:+7924164 Msa0754370:five_prime_UTR
ATGTCGCCTGAAAATACCGT+TGG 0.599343 5_2:+7924571 Msa0754370:CDS
TGCACCCAACGTGTTCTCGA+TGG 0.600223 5_2:-7924536 None:intergenic
TACTTCAATCTGAACTTCCA+AGG 0.601898 5_2:+7925031 Msa0754370:exon
TGTCCCTCAAAAGTGTTGAG+TGG 0.609379 5_2:-7925172 None:intergenic
GGTGAAATTTGAGTTTGTGG+GGG 0.618265 5_2:-7924312 None:intergenic
ACGTTGAGTAGAAGTTTCGA+TGG 0.635634 5_2:-7924511 None:intergenic
TGTTGTTGTTACGAGAAAGG+TGG 0.636326 5_2:-7924354 None:intergenic
AACCTTCACTAATTCGCCAA+CGG 0.655659 5_2:-7924587 None:intergenic
CGTGTCCATCGAGAACACGT+TGG 0.692755 5_2:+7924531 Msa0754370:CDS
CTCATCATCCCTAAACAGTG+AGG 0.694635 5_2:-7924163 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GGATATAAATTATGAAAAAA+GGG - chr5_2:7924741-7924760 None:intergenic 15.0%
!! AGGATATAAATTATGAAAAA+AGG - chr5_2:7924742-7924761 None:intergenic 15.0%
!! AAAAATGATATGAAAAAACA+AGG - chr5_2:7924762-7924781 None:intergenic 15.0%
!! AAGTAAAAAATAATACTGTT+TGG + chr5_2:7925239-7925258 Msa0754370:three_prime_UTR 15.0%
!! GGATATAAATTATGAAAAAA+GGG - chr5_2:7924741-7924760 None:intergenic 15.0%
!! AGGATATAAATTATGAAAAA+AGG - chr5_2:7924742-7924761 None:intergenic 15.0%
!! AAAAATGATATGAAAAAACA+AGG - chr5_2:7924762-7924781 None:intergenic 15.0%
!! AAGTAAAAAATAATACTGTT+TGG + chr5_2:7925239-7925258 Msa0754370:three_prime_UTR 15.0%
!! AAGATATTATTCACACTTAT+TGG + chr5_2:7925090-7925109 Msa0754370:three_prime_UTR 20.0%
!! AATCACTTTAGCATTAATTA+TGG - chr5_2:7925123-7925142 None:intergenic 20.0%
!! AAGATATTATTCACACTTAT+TGG + chr5_2:7925090-7925109 Msa0754370:three_prime_UTR 20.0%
!! AATCACTTTAGCATTAATTA+TGG - chr5_2:7925123-7925142 None:intergenic 20.0%
!! ACTTATATTTCGATTTGTAG+AGG - chr5_2:7924250-7924269 None:intergenic 25.0%
!!! GGTTTTTTTCAAAAGTGAAA+GGG - chr5_2:7924336-7924355 None:intergenic 25.0%
!!! TGGTTTTTTTCAAAAGTGAA+AGG - chr5_2:7924337-7924356 None:intergenic 25.0%
! AATTAATGCTAAAGTGATTG+AGG + chr5_2:7925124-7925143 Msa0754370:three_prime_UTR 25.0%
! AATCTTATGTATCTTGTGAA+AGG + chr5_2:7925196-7925215 Msa0754370:three_prime_UTR 25.0%
! AGGGCAACTAAATATAATTT+GGG - chr5_2:7925391-7925410 None:intergenic 25.0%
! AAGGGCAACTAAATATAATT+TGG - chr5_2:7925392-7925411 None:intergenic 25.0%
!! ACTTATATTTCGATTTGTAG+AGG - chr5_2:7924250-7924269 None:intergenic 25.0%
!!! GGTTTTTTTCAAAAGTGAAA+GGG - chr5_2:7924336-7924355 None:intergenic 25.0%
!!! TGGTTTTTTTCAAAAGTGAA+AGG - chr5_2:7924337-7924356 None:intergenic 25.0%
! AATTAATGCTAAAGTGATTG+AGG + chr5_2:7925124-7925143 Msa0754370:three_prime_UTR 25.0%
! AATCTTATGTATCTTGTGAA+AGG + chr5_2:7925196-7925215 Msa0754370:three_prime_UTR 25.0%
! AGGGCAACTAAATATAATTT+GGG - chr5_2:7925391-7925410 None:intergenic 25.0%
! AAGGGCAACTAAATATAATT+TGG - chr5_2:7925392-7925411 None:intergenic 25.0%
! ATATTTCGATTTGTAGAGGT+TGG - chr5_2:7924246-7924265 None:intergenic 30.0%
AGAGTTTGGATTTCAGTTTA+CGG - chr5_2:7924397-7924416 None:intergenic 30.0%
! TGCATTGATGACTTATGATA+AGG + chr5_2:7924609-7924628 Msa0754370:CDS 30.0%
TAATTACTTTCAAGCGTGAA+TGG - chr5_2:7924694-7924713 None:intergenic 30.0%
ATGAAAAAAGGGAACATTGA+AGG - chr5_2:7924730-7924749 None:intergenic 30.0%
TATACACGATGCATATGATA+AGG - chr5_2:7924894-7924913 None:intergenic 30.0%
TAATCAACCACCAAATGAAA+TGG + chr5_2:7924939-7924958 Msa0754370:intron 30.0%
TAGTGAGAAATTGAAGAACT+TGG + chr5_2:7924977-7924996 Msa0754370:CDS 30.0%
AGTGAGAAATTGAAGAACTT+GGG + chr5_2:7924978-7924997 Msa0754370:CDS 30.0%
ATGTATCTTGTGAAAGGTTA+AGG + chr5_2:7925202-7925221 Msa0754370:three_prime_UTR 30.0%
! AATCTAGCTGCATGTATTTT+TGG + chr5_2:7925271-7925290 Msa0754370:three_prime_UTR 30.0%
AAATTTATGAATCGTCAAGC+TGG + chr5_2:7925330-7925349 Msa0754370:three_prime_UTR 30.0%
ATCCTTTACATGTTGCATAA+GGG - chr5_2:7925410-7925429 None:intergenic 30.0%
TATCCTTTACATGTTGCATA+AGG - chr5_2:7925411-7925430 None:intergenic 30.0%
! ATATTTCGATTTGTAGAGGT+TGG - chr5_2:7924246-7924265 None:intergenic 30.0%
AGAGTTTGGATTTCAGTTTA+CGG - chr5_2:7924397-7924416 None:intergenic 30.0%
! TGCATTGATGACTTATGATA+AGG + chr5_2:7924609-7924628 Msa0754370:CDS 30.0%
TAATTACTTTCAAGCGTGAA+TGG - chr5_2:7924694-7924713 None:intergenic 30.0%
ATGAAAAAAGGGAACATTGA+AGG - chr5_2:7924730-7924749 None:intergenic 30.0%
TATACACGATGCATATGATA+AGG - chr5_2:7924894-7924913 None:intergenic 30.0%
TAATCAACCACCAAATGAAA+TGG + chr5_2:7924939-7924958 Msa0754370:intron 30.0%
TAGTGAGAAATTGAAGAACT+TGG + chr5_2:7924977-7924996 Msa0754370:CDS 30.0%
AGTGAGAAATTGAAGAACTT+GGG + chr5_2:7924978-7924997 Msa0754370:CDS 30.0%
ATGTATCTTGTGAAAGGTTA+AGG + chr5_2:7925202-7925221 Msa0754370:three_prime_UTR 30.0%
! AATCTAGCTGCATGTATTTT+TGG + chr5_2:7925271-7925290 Msa0754370:three_prime_UTR 30.0%
AAATTTATGAATCGTCAAGC+TGG + chr5_2:7925330-7925349 Msa0754370:three_prime_UTR 30.0%
ATCCTTTACATGTTGCATAA+GGG - chr5_2:7925410-7925429 None:intergenic 30.0%
TATCCTTTACATGTTGCATA+AGG - chr5_2:7925411-7925430 None:intergenic 30.0%
!! AGGTTTTGATGTTCATGGAT+TGG - chr5_2:7924146-7924165 None:intergenic 35.0%
ACATCAAAACCTCACTGTTT+AGG + chr5_2:7924154-7924173 Msa0754370:five_prime_UTR 35.0%
CATCAAAACCTCACTGTTTA+GGG + chr5_2:7924155-7924174 Msa0754370:five_prime_UTR 35.0%
TGATGAGGCTATTATTTGTC+AGG + chr5_2:7924179-7924198 Msa0754370:five_prime_UTR 35.0%
! AGGGTGAAATTTGAGTTTGT+GGG - chr5_2:7924317-7924336 None:intergenic 35.0%
! AAGGGTGAAATTTGAGTTTG+TGG - chr5_2:7924318-7924337 None:intergenic 35.0%
! TGTTGTTGTTGTTACGAGAA+AGG - chr5_2:7924360-7924379 None:intergenic 35.0%
ATCCAAACTCTCTTTGTCTT+TGG + chr5_2:7924406-7924425 Msa0754370:five_prime_UTR 35.0%
AACGGAAATTTGTCAGTTTG+AGG - chr5_2:7924457-7924476 None:intergenic 35.0%
TCAAACTGACAAATTTCCGT+TGG + chr5_2:7924456-7924475 Msa0754370:CDS 35.0%
CAAATTTCCGTTGGATACAA+CGG + chr5_2:7924465-7924484 Msa0754370:CDS 35.0%
CTTATGATAAGGAGAAGAGA+AGG + chr5_2:7924620-7924639 Msa0754370:CDS 35.0%
AAGGGAACATTGAAGGATTA+AGG - chr5_2:7924723-7924742 None:intergenic 35.0%
! CATTTGGTGGTTGATTAACA+TGG - chr5_2:7924936-7924955 None:intergenic 35.0%
GTGAGAAATTGAAGAACTTG+GGG + chr5_2:7924979-7924998 Msa0754370:CDS 35.0%
TTGGAAGTTCAGATTGAAGT+AGG - chr5_2:7925032-7925051 None:intergenic 35.0%
TACTTCAATCTGAACTTCCA+AGG + chr5_2:7925031-7925050 Msa0754370:exon 35.0%
GTGACTGATTGAATCTCAAT+TGG + chr5_2:7925149-7925168 Msa0754370:three_prime_UTR 35.0%
TGAGTAGTATGAGGTAGTTT+AGG + chr5_2:7925362-7925381 Msa0754370:three_prime_UTR 35.0%
!! GTTCATGGATTGGTATCTTT+GGG - chr5_2:7924136-7924155 None:intergenic 35.0%
!! TGTTCATGGATTGGTATCTT+TGG - chr5_2:7924137-7924156 None:intergenic 35.0%
!! AGGTTTTGATGTTCATGGAT+TGG - chr5_2:7924146-7924165 None:intergenic 35.0%
ACATCAAAACCTCACTGTTT+AGG + chr5_2:7924154-7924173 Msa0754370:five_prime_UTR 35.0%
CATCAAAACCTCACTGTTTA+GGG + chr5_2:7924155-7924174 Msa0754370:five_prime_UTR 35.0%
TGATGAGGCTATTATTTGTC+AGG + chr5_2:7924179-7924198 Msa0754370:five_prime_UTR 35.0%
! AGGGTGAAATTTGAGTTTGT+GGG - chr5_2:7924317-7924336 None:intergenic 35.0%
! AAGGGTGAAATTTGAGTTTG+TGG - chr5_2:7924318-7924337 None:intergenic 35.0%
! TGTTGTTGTTGTTACGAGAA+AGG - chr5_2:7924360-7924379 None:intergenic 35.0%
ATCCAAACTCTCTTTGTCTT+TGG + chr5_2:7924406-7924425 Msa0754370:five_prime_UTR 35.0%
AACGGAAATTTGTCAGTTTG+AGG - chr5_2:7924457-7924476 None:intergenic 35.0%
TCAAACTGACAAATTTCCGT+TGG + chr5_2:7924456-7924475 Msa0754370:CDS 35.0%
CAAATTTCCGTTGGATACAA+CGG + chr5_2:7924465-7924484 Msa0754370:CDS 35.0%
CTTATGATAAGGAGAAGAGA+AGG + chr5_2:7924620-7924639 Msa0754370:CDS 35.0%
AAGGGAACATTGAAGGATTA+AGG - chr5_2:7924723-7924742 None:intergenic 35.0%
! CATTTGGTGGTTGATTAACA+TGG - chr5_2:7924936-7924955 None:intergenic 35.0%
GTGAGAAATTGAAGAACTTG+GGG + chr5_2:7924979-7924998 Msa0754370:CDS 35.0%
TTGGAAGTTCAGATTGAAGT+AGG - chr5_2:7925032-7925051 None:intergenic 35.0%
TACTTCAATCTGAACTTCCA+AGG + chr5_2:7925031-7925050 Msa0754370:exon 35.0%
GTGACTGATTGAATCTCAAT+TGG + chr5_2:7925149-7925168 Msa0754370:three_prime_UTR 35.0%
TGAGTAGTATGAGGTAGTTT+AGG + chr5_2:7925362-7925381 Msa0754370:three_prime_UTR 35.0%
!! CAGTGAGGTTTTGATGTTCA+TGG - chr5_2:7924151-7924170 None:intergenic 40.0%
ATTATTTGTCAGGTCACTGC+TGG + chr5_2:7924189-7924208 Msa0754370:five_prime_UTR 40.0%
AATGAGTGAAGGAAGCTTCT+AGG - chr5_2:7924288-7924307 None:intergenic 40.0%
! GGTGAAATTTGAGTTTGTGG+GGG - chr5_2:7924315-7924334 None:intergenic 40.0%
! GGGTGAAATTTGAGTTTGTG+GGG - chr5_2:7924316-7924335 None:intergenic 40.0%
! TGTTGTTGTTACGAGAAAGG+TGG - chr5_2:7924357-7924376 None:intergenic 40.0%
AGCCAAAGACAAAGAGAGTT+TGG - chr5_2:7924411-7924430 None:intergenic 40.0%
! TCTCTTTGTCTTTGGCTTCA+TGG + chr5_2:7924414-7924433 Msa0754370:exon 40.0%
! CTCTTTGTCTTTGGCTTCAT+GGG + chr5_2:7924415-7924434 Msa0754370:exon 40.0%
GTCAGTTTGAGGAGTAGAAA+CGG - chr5_2:7924446-7924465 None:intergenic 40.0%
TTGGTTTCCGTTGTATCCAA+CGG - chr5_2:7924475-7924494 None:intergenic 40.0%
AGAAGTTTCGATGGTCTAAG+AGG - chr5_2:7924505-7924524 None:intergenic 40.0%
ACGTTGAGTAGAAGTTTCGA+TGG - chr5_2:7924514-7924533 None:intergenic 40.0%
! AATTCGCCAACGGTATTTTC+AGG - chr5_2:7924580-7924599 None:intergenic 40.0%
AACCTTCACTAATTCGCCAA+CGG - chr5_2:7924590-7924609 None:intergenic 40.0%
GAAGAGAAGGCCATTATTGA+AGG + chr5_2:7924633-7924652 Msa0754370:CDS 40.0%
TGAATGGAGAGAAGTGAAGT+TGG - chr5_2:7924678-7924697 None:intergenic 40.0%
CAAACCTGCACCATTTCATT+TGG - chr5_2:7924952-7924971 None:intergenic 40.0%
ATGAAATGGTGCAGGTTTGA+AGG + chr5_2:7924953-7924972 Msa0754370:intron 40.0%
AATTGAAGAACTTGGGGTCA+AGG + chr5_2:7924985-7925004 Msa0754370:CDS 40.0%
CGAAGCTATTGAGTAGTATG+AGG + chr5_2:7925353-7925372 Msa0754370:three_prime_UTR 40.0%
TGCCCTTATGCAACATGTAA+AGG + chr5_2:7925405-7925424 Msa0754370:three_prime_UTR 40.0%
!! CAGTGAGGTTTTGATGTTCA+TGG - chr5_2:7924151-7924170 None:intergenic 40.0%
ATTATTTGTCAGGTCACTGC+TGG + chr5_2:7924189-7924208 Msa0754370:five_prime_UTR 40.0%
AATGAGTGAAGGAAGCTTCT+AGG - chr5_2:7924288-7924307 None:intergenic 40.0%
! GGTGAAATTTGAGTTTGTGG+GGG - chr5_2:7924315-7924334 None:intergenic 40.0%
! GGGTGAAATTTGAGTTTGTG+GGG - chr5_2:7924316-7924335 None:intergenic 40.0%
! TGTTGTTGTTACGAGAAAGG+TGG - chr5_2:7924357-7924376 None:intergenic 40.0%
AGCCAAAGACAAAGAGAGTT+TGG - chr5_2:7924411-7924430 None:intergenic 40.0%
! TCTCTTTGTCTTTGGCTTCA+TGG + chr5_2:7924414-7924433 Msa0754370:exon 40.0%
! CTCTTTGTCTTTGGCTTCAT+GGG + chr5_2:7924415-7924434 Msa0754370:exon 40.0%
GTCAGTTTGAGGAGTAGAAA+CGG - chr5_2:7924446-7924465 None:intergenic 40.0%
TTGGTTTCCGTTGTATCCAA+CGG - chr5_2:7924475-7924494 None:intergenic 40.0%
AGAAGTTTCGATGGTCTAAG+AGG - chr5_2:7924505-7924524 None:intergenic 40.0%
ACGTTGAGTAGAAGTTTCGA+TGG - chr5_2:7924514-7924533 None:intergenic 40.0%
! AATTCGCCAACGGTATTTTC+AGG - chr5_2:7924580-7924599 None:intergenic 40.0%
AACCTTCACTAATTCGCCAA+CGG - chr5_2:7924590-7924609 None:intergenic 40.0%
GAAGAGAAGGCCATTATTGA+AGG + chr5_2:7924633-7924652 Msa0754370:CDS 40.0%
TGAATGGAGAGAAGTGAAGT+TGG - chr5_2:7924678-7924697 None:intergenic 40.0%
CAAACCTGCACCATTTCATT+TGG - chr5_2:7924952-7924971 None:intergenic 40.0%
ATGAAATGGTGCAGGTTTGA+AGG + chr5_2:7924953-7924972 Msa0754370:intron 40.0%
AATTGAAGAACTTGGGGTCA+AGG + chr5_2:7924985-7925004 Msa0754370:CDS 40.0%
CGAAGCTATTGAGTAGTATG+AGG + chr5_2:7925353-7925372 Msa0754370:three_prime_UTR 40.0%
TGCCCTTATGCAACATGTAA+AGG + chr5_2:7925405-7925424 Msa0754370:three_prime_UTR 40.0%
CTCATCATCCCTAAACAGTG+AGG - chr5_2:7924166-7924185 None:intergenic 45.0%
CTCACTGTTTAGGGATGATG+AGG + chr5_2:7924164-7924183 Msa0754370:five_prime_UTR 45.0%
CAGGTCACTGCTGGAAAATT+CGG + chr5_2:7924198-7924217 Msa0754370:five_prime_UTR 45.0%
AGGTCACTGCTGGAAAATTC+GGG + chr5_2:7924199-7924218 Msa0754370:five_prime_UTR 45.0%
! TTTGTCTTTGGCTTCATGGG+TGG + chr5_2:7924418-7924437 Msa0754370:exon 45.0%
TGGTCTAAGAGGAGAGTCAT+TGG - chr5_2:7924494-7924513 None:intergenic 45.0%
ATGTCGCCTGAAAATACCGT+TGG + chr5_2:7924571-7924590 Msa0754370:CDS 45.0%
TACCGTTGGCGAATTAGTGA+AGG + chr5_2:7924585-7924604 Msa0754370:CDS 45.0%
! GGATCGGTGTCCTTCAATAA+TGG - chr5_2:7924646-7924665 None:intergenic 45.0%
!! AAGTTGGTAGCGGTTTGGAT+CGG - chr5_2:7924662-7924681 None:intergenic 45.0%
!! AAGTGAAGTTGGTAGCGGTT+TGG - chr5_2:7924667-7924686 None:intergenic 45.0%
!! GAGAGAAGTGAAGTTGGTAG+CGG - chr5_2:7924672-7924691 None:intergenic 45.0%
ACCACCAAATGAAATGGTGC+AGG + chr5_2:7924945-7924964 Msa0754370:intron 45.0%
ACCTGCACCATTTCATTTGG+TGG - chr5_2:7924949-7924968 None:intergenic 45.0%
TGAACTTCCAAGGCAGCATA+TGG + chr5_2:7925041-7925060 Msa0754370:exon 45.0%
TCAATTGCCATATGCTGCCT+TGG - chr5_2:7925051-7925070 None:intergenic 45.0%
! TTGGCCACTCAACACTTTTG+AGG + chr5_2:7925168-7925187 Msa0754370:three_prime_UTR 45.0%
! TGGCCACTCAACACTTTTGA+GGG + chr5_2:7925169-7925188 Msa0754370:three_prime_UTR 45.0%
!! TGTCCCTCAAAAGTGTTGAG+TGG - chr5_2:7925175-7925194 None:intergenic 45.0%
CTCATCATCCCTAAACAGTG+AGG - chr5_2:7924166-7924185 None:intergenic 45.0%
CTCACTGTTTAGGGATGATG+AGG + chr5_2:7924164-7924183 Msa0754370:five_prime_UTR 45.0%
CAGGTCACTGCTGGAAAATT+CGG + chr5_2:7924198-7924217 Msa0754370:five_prime_UTR 45.0%
AGGTCACTGCTGGAAAATTC+GGG + chr5_2:7924199-7924218 Msa0754370:five_prime_UTR 45.0%
! TTTGTCTTTGGCTTCATGGG+TGG + chr5_2:7924418-7924437 Msa0754370:exon 45.0%
TGGTCTAAGAGGAGAGTCAT+TGG - chr5_2:7924494-7924513 None:intergenic 45.0%
ATGTCGCCTGAAAATACCGT+TGG + chr5_2:7924571-7924590 Msa0754370:CDS 45.0%
TACCGTTGGCGAATTAGTGA+AGG + chr5_2:7924585-7924604 Msa0754370:CDS 45.0%
! GGATCGGTGTCCTTCAATAA+TGG - chr5_2:7924646-7924665 None:intergenic 45.0%
!! AAGTTGGTAGCGGTTTGGAT+CGG - chr5_2:7924662-7924681 None:intergenic 45.0%
!! AAGTGAAGTTGGTAGCGGTT+TGG - chr5_2:7924667-7924686 None:intergenic 45.0%
!! GAGAGAAGTGAAGTTGGTAG+CGG - chr5_2:7924672-7924691 None:intergenic 45.0%
ACCACCAAATGAAATGGTGC+AGG + chr5_2:7924945-7924964 Msa0754370:intron 45.0%
ACCTGCACCATTTCATTTGG+TGG - chr5_2:7924949-7924968 None:intergenic 45.0%
TGAACTTCCAAGGCAGCATA+TGG + chr5_2:7925041-7925060 Msa0754370:exon 45.0%
TCAATTGCCATATGCTGCCT+TGG - chr5_2:7925051-7925070 None:intergenic 45.0%
! TTGGCCACTCAACACTTTTG+AGG + chr5_2:7925168-7925187 Msa0754370:three_prime_UTR 45.0%
! TGGCCACTCAACACTTTTGA+GGG + chr5_2:7925169-7925188 Msa0754370:three_prime_UTR 45.0%
!! TGTCCCTCAAAAGTGTTGAG+TGG - chr5_2:7925175-7925194 None:intergenic 45.0%
GTGGGGGCTTAAATGAGTGA+AGG - chr5_2:7924299-7924318 None:intergenic 50.0%
GTGTCCATCGAGAACACGTT+GGG + chr5_2:7924532-7924551 Msa0754370:CDS 50.0%
GTGGGGGCTTAAATGAGTGA+AGG - chr5_2:7924299-7924318 None:intergenic 50.0%
GTGTCCATCGAGAACACGTT+GGG + chr5_2:7924532-7924551 Msa0754370:CDS 50.0%
CGTGTCCATCGAGAACACGT+TGG + chr5_2:7924531-7924550 Msa0754370:CDS 55.0%
TGCACCCAACGTGTTCTCGA+TGG - chr5_2:7924539-7924558 None:intergenic 55.0%
CGTGTCCATCGAGAACACGT+TGG + chr5_2:7924531-7924550 Msa0754370:CDS 55.0%
TGCACCCAACGTGTTCTCGA+TGG - chr5_2:7924539-7924558 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 7924136 7925442 7924136 ID=Msa0754370;Name=Msa0754370
chr5_2 mRNA 7924136 7925442 7924136 ID=Msa0754370-mRNA-1;Parent=Msa0754370;Name=Msa0754370-mRNA-1;_AED=0.00;_eAED=0.00;_QI=297|1|1|1|1|1|2|399|116
chr5_2 exon 7924136 7924706 7924136 ID=Msa0754370-mRNA-1:exon:3572;Parent=Msa0754370-mRNA-1
chr5_2 exon 7924967 7925442 7924967 ID=Msa0754370-mRNA-1:exon:3573;Parent=Msa0754370-mRNA-1
chr5_2 five_prime_UTR 7924136 7924432 7924136 ID=Msa0754370-mRNA-1:five_prime_utr;Parent=Msa0754370-mRNA-1
chr5_2 CDS 7924433 7924706 7924433 ID=Msa0754370-mRNA-1:cds;Parent=Msa0754370-mRNA-1
chr5_2 CDS 7924967 7925043 7924967 ID=Msa0754370-mRNA-1:cds;Parent=Msa0754370-mRNA-1
chr5_2 three_prime_UTR 7925044 7925442 7925044 ID=Msa0754370-mRNA-1:three_prime_utr;Parent=Msa0754370-mRNA-1
Gene Sequence

>Msa0754370

ATGGGTGGTACCGTTTCTACTCCTCAAACTGACAAATTTCCGTTGGATACAACGGAAACCAATGACTCTCCTCTTAGACCATCGAAACTTCTACTCAACGTGTCCATCGAGAACACGTTGGGTGCAATACAAGTTTTGATGTCGCCTGAAAATACCGTTGGCGAATTAGTGAAGGTTGCATTGATGACTTATGATAAGGAGAAGAGAAGGCCATTATTGAAGGACACCGATCCAAACCGCTACCAACTTCACTTCTCTCCATTCACGCTTGAAAGTTTGAAGGTTAGTGAGAAATTGAAGAACTTGGGGTCAAGGAACTTCTTTCTATGCTCAAAGCCTACTTCAATCTGA
Protein sequence

>Msa0754370

MGGTVSTPQTDKFPLDTTETNDSPLRPSKLLLNVSIENTLGAIQVLMSPENTVGELVKVALMTYDKEKRRPLLKDTDPNRYQLHFSPFTLESLKVSEKLKNLGSRNFFLCSKPTSI*