AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1421620

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 68 sgRNAs with CRISPR-Local

Find 269 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTTCCTCCTTCAAGAATTC+TGG 0.172529 tig0025281:-1039 Msa1421620:three_prime_UTR
TTCCCTCATGTAGTATTTAA+AGG 0.173459 tig0025281:+738 None:intergenic
TTACAATGATAATGTGTATT+TGG 0.176865 tig0025281:+3215 None:intergenic
TACAGTGATAATGTGTATTA+TGG 0.178827 tig0025281:+1468 None:intergenic
ATTAAACTTGGAAATGAATT+AGG 0.210390 tig0025281:-4017 Msa1421620:intron
TAATTCATTTCCAAGTTTAA+TGG 0.234342 tig0025281:+4019 None:intergenic
CAGAATTTGCAGTGCAGCGC+AGG 0.294148 tig0025281:+645 None:intergenic
TAATTATAAATATAATTGTT+AGG 0.307154 tig0025281:-1152 Msa1421620:three_prime_UTR
GACTCTGCATAAGACAATAA+TGG 0.355828 tig0025281:-1185 Msa1421620:three_prime_UTR
GTTGAGGATGTTGGCATAGT+TGG 0.363283 tig0025281:-4797 Msa1421620:CDS
TAGAGTCTGAGATTGAGTTT+TGG 0.378664 tig0025281:-5397 Msa1421620:CDS
AGACAATTCCGCAAAGCGTT+TGG 0.379036 tig0025281:+1555 None:intergenic
AACCTAATGTCGGGATTTGC+GGG 0.387922 tig0025281:-5434 None:intergenic
CTGCAGATGCAGATGTTGAT+AGG 0.392164 tig0025281:-625 Msa1421620:three_prime_UTR
CAAAATGATGCCATTAAACT+TGG 0.402093 tig0025281:-4029 Msa1421620:CDS
TAGTACAAAACAGAGGTATA+TGG 0.414342 tig0025281:+1229 None:intergenic
CGAAAGAGCCTTGGAGAGAA+AGG 0.423381 tig0025281:-714 Msa1421620:three_prime_UTR
AGAAAGAGCCTTGGAGAGAA+AGG 0.426750 tig0025281:-669 Msa1421620:three_prime_UTR
ACTCTGCATAAGACAATAAT+GGG 0.428670 tig0025281:-1184 Msa1421620:three_prime_UTR
AGATGTTGATAGGTTCATAT+AGG 0.436481 tig0025281:-615 Msa1421620:three_prime_UTR
GCCTTCATACTGCTTTCTGC+TGG 0.438656 tig0025281:-1065 Msa1421620:three_prime_UTR
AATTATAAATATAATTGTTA+GGG 0.440124 tig0025281:-1151 Msa1421620:three_prime_UTR
GTTCCTCCTTCAAGAATTCT+GGG 0.442364 tig0025281:-1038 Msa1421620:three_prime_UTR
ACAGTGATAATGTGTATTAT+GGG 0.445189 tig0025281:+1469 None:intergenic
TTGTCTCCCAGAATTCTTGA+AGG 0.447191 tig0025281:+1032 None:intergenic
GCTGCTATCCAAACGCTTTG+CGG 0.447265 tig0025281:-1563 Msa1421620:CDS
CTCAAGCTTGTTGCATATGT+TGG 0.452344 tig0025281:-2280 Msa1421620:CDS
GAACCTAATGTCGGGATTTG+CGG 0.452758 tig0025281:-5435 None:intergenic
CAACTAAGAGCTTATCTCAT+TGG 0.455962 tig0025281:+915 None:intergenic
CAGGAGAGCACGTGTCTTTG+TGG 0.456160 tig0025281:-4084 Msa1421620:CDS
CATAGTTGGTGCTCCAATGC+AGG 0.464430 tig0025281:-4783 Msa1421620:CDS
TTGGAGAGAAAGGAAGGGCT+TGG 0.464845 tig0025281:-704 Msa1421620:three_prime_UTR
GGCTACTCTTCCTTCCTGCC+AGG 0.465761 tig0025281:+4054 None:intergenic
GTTAGAACCCACGCTGGTTG+AGG 0.491363 tig0025281:-4813 Msa1421620:CDS
AAGCCCTTCCTTTCTCTCCA+AGG 0.495149 tig0025281:+706 None:intergenic
TTTGCGGAATTGTCTGATGA+AGG 0.504707 tig0025281:-1547 Msa1421620:CDS
CTTCATCTCATAGTTTCTCA+AGG 0.506698 tig0025281:+3290 None:intergenic
TGAGGGAAGCGAAAGAGCCT+TGG 0.511561 tig0025281:-723 Msa1421620:three_prime_UTR
CGTTTGGATAGCAGCCAAGC+AGG 0.516208 tig0025281:+1571 None:intergenic
CTTGAGAAACTATGAGATGA+AGG 0.518610 tig0025281:-3289 Msa1421620:CDS
CCCACGCTGGTTGAGGATGT+TGG 0.525901 tig0025281:-4806 Msa1421620:CDS
GCGCAGGGCCTTTCTCTCCA+AGG 0.526608 tig0025281:+661 None:intergenic
AATCGATTGAACCTAATGTC+GGG 0.528587 tig0025281:-5443 None:intergenic
TGTCTTTGTGGAAGTAGTCC+TGG 0.529821 tig0025281:-4072 Msa1421620:CDS
TTGCCTTTAAATACTACATG+AGG 0.534140 tig0025281:-741 Msa1421620:three_prime_UTR
TTCTTGTCGACATACCTGCT+TGG 0.538298 tig0025281:-1585 Msa1421620:CDS
AGAGCCTTGGAGAGAAAGGA+AGG 0.541262 tig0025281:-710 Msa1421620:three_prime_UTR
GTGAGAGAGAGAAAGAGCCT+TGG 0.551422 tig0025281:-678 Msa1421620:three_prime_UTR
GCCAGCAGAAAGCAGTATGA+AGG 0.568670 tig0025281:+1064 None:intergenic
AGAATTTGCAGTGCAGCGCA+GGG 0.576307 tig0025281:+646 None:intergenic
CATACTGCTTTCTGCTGGCG+TGG 0.581915 tig0025281:-1060 Msa1421620:three_prime_UTR
TTTGTGGAAGTAGTCCTGGC+AGG 0.584999 tig0025281:-4068 Msa1421620:CDS
CAAAATGTGATGGCAAGGCA+AGG 0.585482 tig0025281:-767 Msa1421620:three_prime_UTR
AGAGGCATAAAGTTGCATGA+TGG 0.590712 tig0025281:-1006 Msa1421620:three_prime_UTR
TCTCCCAGAATTCTTGAAGG+AGG 0.595273 tig0025281:+1035 None:intergenic
TGGAAGTAGTCCTGGCAGGA+AGG 0.600387 tig0025281:-4064 Msa1421620:CDS
ATAGTTGGTGCTCCAATGCA+GGG 0.604033 tig0025281:-4782 Msa1421620:CDS
TGCCTTTAAATACTACATGA+GGG 0.610711 tig0025281:-740 Msa1421620:three_prime_UTR
GCTTGCAAAATGTGATGGCA+AGG 0.615832 tig0025281:-772 Msa1421620:three_prime_UTR
ATCACATCTGCAGATTTGTG+TGG 0.619954 tig0025281:+1522 None:intergenic
ACAAATCTGCAGATGTGATG+AGG 0.630753 tig0025281:-1518 Msa1421620:CDS
GCCAACATCCTCAACCAGCG+TGG 0.639391 tig0025281:+4805 None:intergenic
GAGCCTTGGAGAGAAAGGAA+GGG 0.646139 tig0025281:-709 Msa1421620:three_prime_UTR
CCAACATCCTCAACCAGCGT+GGG 0.654114 tig0025281:+4806 None:intergenic
AGCTGTCAAAGCTACTGATG+AGG 0.663227 tig0025281:-3266 Msa1421620:CDS
AATTCTGGGAGACAAAGAAG+AGG 0.676675 tig0025281:-1024 Msa1421620:three_prime_UTR
TTTGGGGTTAGAACCCACGC+TGG 0.715063 tig0025281:-4819 Msa1421620:CDS
ACATTCTTAGTACAAAACAG+AGG 0.764301 tig0025281:+1222 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAATTTAAATTATTTAAT+TGG - tig0025281:3932-3951 Msa1421620:intron 0.0%
!!! ATTAATTTTATTAATTTTAT+TGG - tig0025281:4299-4318 Msa1421620:intron 0.0%
!! AGAATATATTGTTAATATTT+AGG - tig0025281:1551-1570 Msa1421620:CDS 10.0%
!!! ATTGGTAAAATTATAATTTT+TGG - tig0025281:3124-3143 Msa1421620:intron 10.0%
!! GAAATAAAATGAAAAAAATT+TGG + tig0025281:3292-3311 None:intergenic 10.0%
!! AAATGAAAAATAAAAAAGGA+CGG - tig0025281:701-720 Msa1421620:three_prime_UTR 15.0%
!! ATGAATAAATAATAATGGTT+AGG - tig0025281:746-765 Msa1421620:three_prime_UTR 15.0%
!! TGAATAAATAATAATGGTTA+GGG - tig0025281:747-766 Msa1421620:three_prime_UTR 15.0%
!!! TTAATATTTAGGCTTTATTT+GGG - tig0025281:1562-1581 Msa1421620:CDS 15.0%
!!! ATGAATACTTTTGTTTTTTA+CGG - tig0025281:1608-1627 Msa1421620:intron 15.0%
!! AAACATAGTTTGAAAAAAAA+GGG + tig0025281:1771-1790 None:intergenic 15.0%
!!! TTTTTTTTATCTGTTTTTTG+TGG - tig0025281:2045-2064 Msa1421620:intron 15.0%
!! TATTTCAATTGTTAAATGAA+AGG + tig0025281:2557-2576 None:intergenic 15.0%
!! TGAAGCAAAAATTAATAAAA+TGG - tig0025281:2610-2629 Msa1421620:intron 15.0%
!!! ATTTAATTTAAATATGTGTG+TGG + tig0025281:3918-3937 None:intergenic 15.0%
!! ATTGGTAAAATTATAATTTC+TGG - tig0025281:4340-4359 Msa1421620:intron 15.0%
!!! TATTATTTTTAACTTGCAAT+TGG - tig0025281:4670-4689 Msa1421620:intron 15.0%
!! AATGAAAAATAAAAAAGGAC+GGG - tig0025281:702-721 Msa1421620:three_prime_UTR 20.0%
!!! TCAAAGTTTTTAAGTCAAAA+AGG + tig0025281:810-829 None:intergenic 20.0%
!! ATCTAAGTTATACATGATTT+AGG - tig0025281:858-877 Msa1421620:three_prime_UTR 20.0%
!! AGTATGAAATGTTAACTTTA+TGG - tig0025281:1445-1464 Msa1421620:CDS 20.0%
!!! GTTAATATTTAGGCTTTATT+TGG - tig0025281:1561-1580 Msa1421620:CDS 20.0%
!!! TAATATTTAGGCTTTATTTG+GGG - tig0025281:1563-1582 Msa1421620:CDS 20.0%
!!! TGAATACTTTTGTTTTTTAC+GGG - tig0025281:1609-1628 Msa1421620:intron 20.0%
!! CAAACATAGTTTGAAAAAAA+AGG + tig0025281:1772-1791 None:intergenic 20.0%
!!! TTTGTAACATTTTTTAGCTA+TGG + tig0025281:1798-1817 None:intergenic 20.0%
!! TAATTCATTTCCAAGTTTAA+TGG + tig0025281:1990-2009 None:intergenic 20.0%
!! ATTAAACTTGGAAATGAATT+AGG - tig0025281:1989-2008 Msa1421620:intron 20.0%
!!! TTACAATGATAATGTGTATT+TGG + tig0025281:2794-2813 None:intergenic 20.0%
!!! TTATTATTTTTAGCTTGCAA+TGG - tig0025281:2960-2979 Msa1421620:intron 20.0%
!!! TATTATTTTTAGCTTGCAAT+GGG - tig0025281:2961-2980 Msa1421620:intron 20.0%
!!! AAAAATGGGTAATGTTTTTT+CGG - tig0025281:3169-3188 Msa1421620:intron 20.0%
!! ATTCAATTGATGAAATGAAA+AGG + tig0025281:3450-3469 None:intergenic 20.0%
!! AGAAGTTTGAACAAATAAAT+CGG + tig0025281:3888-3907 None:intergenic 20.0%
!! AACATGATTAACCATTATAA+TGG - tig0025281:4066-4085 Msa1421620:CDS 20.0%
!! TTATATGTAAGGAAAATATG+AGG + tig0025281:4183-4202 None:intergenic 20.0%
!! TCATTCAATCATTATATGTA+AGG + tig0025281:4194-4213 None:intergenic 20.0%
! ATGAAAAATAAAAAAGGACG+GGG - tig0025281:703-722 Msa1421620:three_prime_UTR 25.0%
!! TTAATACTATGTTTTGCCAT+TGG - tig0025281:1024-1043 Msa1421620:three_prime_UTR 25.0%
! GAAAATGAACTCAAAGTTAA+GGG + tig0025281:1109-1128 None:intergenic 25.0%
! AGAAAATGAACTCAAAGTTA+AGG + tig0025281:1110-1129 None:intergenic 25.0%
!! ATTTTCCTAAGAGTTTAAGA+AGG - tig0025281:1143-1162 Msa1421620:three_prime_UTR 25.0%
!! GAAAATATGAAGAGTATAAG+AGG + tig0025281:1384-1403 None:intergenic 25.0%
! AAATGTTAACTTTATGGACA+TGG - tig0025281:1451-1470 Msa1421620:CDS 25.0%
! TTCTTACATGTAGTAGTATT+TGG + tig0025281:1535-1554 None:intergenic 25.0%
!!! GAATACTTTTGTTTTTTACG+GGG - tig0025281:1610-1629 Msa1421620:intron 25.0%
!!! TTTTTTCTTCCCACATTTTA+AGG - tig0025281:1691-1710 Msa1421620:intron 25.0%
! AGAAGACAAAAAGTAAGATA+AGG - tig0025281:1888-1907 Msa1421620:intron 25.0%
! AGATAAGGTTATAAAAGTTC+AGG - tig0025281:1903-1922 Msa1421620:intron 25.0%
!! CAAAACTCAAAGTATTAACA+AGG + tig0025281:2236-2255 None:intergenic 25.0%
! TTCAAAAGCAATGTTGTAAA+GGG + tig0025281:2288-2307 None:intergenic 25.0%
! ATTCAAAAGCAATGTTGTAA+AGG + tig0025281:2289-2308 None:intergenic 25.0%
!! AGAATTTGAGTTAAAGTCAA+AGG + tig0025281:2325-2344 None:intergenic 25.0%
!! AAGTTTTCAAAAGATGGTTA+TGG - tig0025281:2424-2443 Msa1421620:intron 25.0%
!! ATAAGATATTAGCTTCAACA+AGG + tig0025281:2653-2672 None:intergenic 25.0%
! TTGTTGAAGCTAATATCTTA+TGG - tig0025281:2652-2671 Msa1421620:intron 25.0%
! TACTAGGAGAGTTTATTATA+AGG + tig0025281:3027-3046 None:intergenic 25.0%
!!! TAATTTTTGGAGACATCTTA+TGG - tig0025281:3137-3156 Msa1421620:intron 25.0%
!!! TTATGGATTAGACAGAAAAA+TGG - tig0025281:3154-3173 Msa1421620:intron 25.0%
!!! TATGGATTAGACAGAAAAAT+GGG - tig0025281:3155-3174 Msa1421620:intron 25.0%
!! AAAAATTTGGATACCCATTT+TGG + tig0025281:3279-3298 None:intergenic 25.0%
! TATTCATCGTTATCAAAAGA+AGG + tig0025281:3333-3352 None:intergenic 25.0%
!!! TAACGATGAATACCTTTTTT+CGG - tig0025281:3341-3360 Msa1421620:intron 25.0%
!!! TTAATACATTGTTTTGCCTT+TGG - tig0025281:3583-3602 Msa1421620:intron 25.0%
! AGAAAATAAACTCAAAGTGA+AGG + tig0025281:3638-3657 None:intergenic 25.0%
!!! AAGTTTTCAAATGTTGGTTA+TGG - tig0025281:4011-4030 Msa1421620:intron 25.0%
!! TACTATGAGAGTTTTCTATA+AGG + tig0025281:4121-4140 None:intergenic 25.0%
!! ACAGTGATAATGTGTATTAT+GGG + tig0025281:4540-4559 None:intergenic 25.0%
!! TACAGTGATAATGTGTATTA+TGG + tig0025281:4541-4560 None:intergenic 25.0%
! CAATTGTAGAGTATGAAAAA+TGG - tig0025281:5143-5162 Msa1421620:intron 25.0%
!!! GTAAAAAGAAGCATAGATTT+TGG - tig0025281:5212-5231 Msa1421620:intron 25.0%
!!! TTCATATAGGTTTTATGTTG+TGG - tig0025281:5404-5423 Msa1421620:CDS 25.0%
!!! TCATATAGGTTTTATGTTGT+GGG - tig0025281:5405-5424 Msa1421620:CDS 25.0%
!!! ATCTCATTTTGAAGCTGTAT+GGG + tig0025281:669-688 None:intergenic 30.0%
!!! AATCTCATTTTGAAGCTGTA+TGG + tig0025281:670-689 None:intergenic 30.0%
GAAAATCTAAGAACACGATA+GGG + tig0025281:1079-1098 None:intergenic 30.0%
ATCAACCTTCTTAAACTCTT+AGG + tig0025281:1151-1170 None:intergenic 30.0%
!! AAGGTTGATTTCTTTTGAAC+AGG - tig0025281:1162-1181 Msa1421620:three_prime_UTR 30.0%
! ATTTCTTTTGAACAGGTGTT+TGG - tig0025281:1169-1188 Msa1421620:three_prime_UTR 30.0%
! TTTCTTTTGAACAGGTGTTT+GGG - tig0025281:1170-1189 Msa1421620:three_prime_UTR 30.0%
TTAACTTTATGGACATGGTA+AGG - tig0025281:1456-1475 Msa1421620:CDS 30.0%
TAACTTTATGGACATGGTAA+GGG - tig0025281:1457-1476 Msa1421620:CDS 30.0%
TTACATGTAGTAGTATTTGG+TGG + tig0025281:1532-1551 None:intergenic 30.0%
AAATACAGCTATAGCAGAAA+AGG + tig0025281:1717-1736 None:intergenic 30.0%
AGACGATTTACAAATAGTCT+TGG + tig0025281:1827-1846 None:intergenic 30.0%
! ATCGTCTTTTGTGAAACTTT+TGG - tig0025281:1840-1859 Msa1421620:intron 30.0%
! GTTTAATGGCATCATTTTGA+TGG + tig0025281:1976-1995 None:intergenic 30.0%
CAAAATGATGCCATTAAACT+TGG - tig0025281:1977-1996 Msa1421620:intron 30.0%
!!! TTATCTGTTTTTTGTGGAAG+AGG - tig0025281:2051-2070 Msa1421620:intron 30.0%
!! TACAACATTGCTTTTGAATC+TGG - tig0025281:2290-2309 Msa1421620:CDS 30.0%
TTTGTTACACAAGGTTTCAT+GGG + tig0025281:2368-2387 None:intergenic 30.0%
!! CCATCTTTTGAAAACTTTTG+TGG + tig0025281:2421-2440 None:intergenic 30.0%
! CCACAAAAGTTTTCAAAAGA+TGG - tig0025281:2418-2437 Msa1421620:intron 30.0%
TTTAATCTACACAAATCTGC+AGG - tig0025281:2688-2707 Msa1421620:intron 30.0%
AATGAGAAAGAACACAAAAG+TGG + tig0025281:2892-2911 None:intergenic 30.0%
TCTTACAATAGACACATGTT+TGG - tig0025281:3097-3116 Msa1421620:intron 30.0%
GAAAAAGGAATACCGAAAAA+AGG + tig0025281:3356-3375 None:intergenic 30.0%
CACAAATTCCACATAATCAA+GGG + tig0025281:3507-3526 None:intergenic 30.0%
TAAAAGCAATACAAAGCCAA+AGG + tig0025281:3602-3621 None:intergenic 30.0%
AGTGTTGTAAAGGGAAAATT+TGG + tig0025281:3820-3839 None:intergenic 30.0%
TTTGGAAGTAGTGTTGTAAA+GGG + tig0025281:3829-3848 None:intergenic 30.0%
ATTTGGAAGTAGTGTTGTAA+AGG + tig0025281:3830-3849 None:intergenic 30.0%
TACAACACTACTTCCAAATT+TGG - tig0025281:3831-3850 Msa1421620:intron 30.0%
AAGGAAACAACAACCAAATT+TGG + tig0025281:3847-3866 None:intergenic 30.0%
!! TAAATCGGATTTTGAGTCAT+AGG + tig0025281:3873-3892 None:intergenic 30.0%
! GAAAACTTTTGAGCATATGT+TGG + tig0025281:3999-4018 None:intergenic 30.0%
! GCTCAAAAGTTTTCAAATGT+TGG - tig0025281:4005-4024 Msa1421620:intron 30.0%
AGGTATGGCAAAAGAAAAAT+TGG + tig0025281:4101-4120 None:intergenic 30.0%
! TGAGAGTTTTCTATAAGGTA+TGG + tig0025281:4116-4135 None:intergenic 30.0%
GAAAATATGAGGAATCCAAA+AGG + tig0025281:4172-4191 None:intergenic 30.0%
AGACACATGTTTAGTAACAT+TGG - tig0025281:4322-4341 Msa1421620:intron 30.0%
!! TTGATTGTTTTGTGCAGAAT+AGG - tig0025281:4696-4715 Msa1421620:intron 30.0%
!! TGATTGTTTTGTGCAGAATA+GGG - tig0025281:4697-4716 Msa1421620:intron 30.0%
! TAGTACAAAACAGAGGTATA+TGG + tig0025281:4780-4799 None:intergenic 30.0%
ACATTCTTAGTACAAAACAG+AGG + tig0025281:4787-4806 None:intergenic 30.0%
ACTCTGCATAAGACAATAAT+GGG - tig0025281:4822-4841 Msa1421620:CDS 30.0%
TTGCCTTTAAATACTACATG+AGG - tig0025281:5265-5284 Msa1421620:intron 30.0%
TGCCTTTAAATACTACATGA+GGG - tig0025281:5266-5285 Msa1421620:intron 30.0%
TTCCCTCATGTAGTATTTAA+AGG + tig0025281:5271-5290 None:intergenic 30.0%
! AGATGTTGATAGGTTCATAT+AGG - tig0025281:5391-5410 Msa1421620:CDS 30.0%
!! TAGAGTCTGAGATTGAGTTT+TGG - tig0025281:609-628 Msa1421620:three_prime_UTR 35.0%
!!! AGTTTTGGAACGTCTTGTTT+TGG - tig0025281:624-643 Msa1421620:three_prime_UTR 35.0%
!! TTGGTAATACACTCCTCTTT+TGG - tig0025281:643-662 Msa1421620:three_prime_UTR 35.0%
AAATAAAAAAGGACGGGGAA+TGG - tig0025281:708-727 Msa1421620:three_prime_UTR 35.0%
AATAAAAAAGGACGGGGAAT+GGG - tig0025281:709-728 Msa1421620:three_prime_UTR 35.0%
GGAAAATCTAAGAACACGAT+AGG + tig0025281:1080-1099 None:intergenic 35.0%
ACTCAAAGTTAAGGGAAAGT+GGG + tig0025281:1101-1120 None:intergenic 35.0%
AACTCAAAGTTAAGGGAAAG+TGG + tig0025281:1102-1121 None:intergenic 35.0%
! TTCTTTTGAACAGGTGTTTG+GGG - tig0025281:1171-1190 Msa1421620:three_prime_UTR 35.0%
AAGTCACATGTGACAAATTC+AGG - tig0025281:1280-1299 Msa1421620:three_prime_UTR 35.0%
AAATTCAGGAAACCCATTCA+TGG - tig0025281:1294-1313 Msa1421620:three_prime_UTR 35.0%
CATTTCATACTGTTCGTTTC+AGG + tig0025281:1436-1455 None:intergenic 35.0%
GGATATGCAAGTGAATAAGA+TGG - tig0025281:1492-1511 Msa1421620:CDS 35.0%
!! ATTTAGGCTTTATTTGGGGA+AGG - tig0025281:1567-1586 Msa1421620:CDS 35.0%
!!! ACTTTTGTTTTTTACGGGGA+AGG - tig0025281:1614-1633 Msa1421620:intron 35.0%
CTTTATATTCCTGACCTACA+CGG - tig0025281:1658-1677 Msa1421620:intron 35.0%
CAGAAAAGGCCTTAAAATGT+GGG + tig0025281:1703-1722 None:intergenic 35.0%
AATTCGTTGCAAACTTCAGT+TGG - tig0025281:2116-2135 Msa1421620:intron 35.0%
ATTCGTTGCAAACTTCAGTT+GGG - tig0025281:2117-2136 Msa1421620:intron 35.0%
! GTTTGTTACACAAGGTTTCA+TGG + tig0025281:2369-2388 None:intergenic 35.0%
GACTAAGAGAGTCGATTAAA+AGG + tig0025281:2484-2503 None:intergenic 35.0%
CTTCATCTCATAGTTTCTCA+AGG + tig0025281:2719-2738 None:intergenic 35.0%
CTTGAGAAACTATGAGATGA+AGG - tig0025281:2717-2736 Msa1421620:intron 35.0%
AGAAAGAACACAAAAGTGGA+AGG + tig0025281:2888-2907 None:intergenic 35.0%
!!! TTTTAGCTTGCAATGGGTTA+TGG - tig0025281:2967-2986 Msa1421620:intron 35.0%
! TTCACGTTCACACTTTTACT+AGG + tig0025281:3043-3062 None:intergenic 35.0%
AAAAATATGCAGAGTCCAAG+AGG + tig0025281:3078-3097 None:intergenic 35.0%
! AGACACATGTTTGGTAACAT+TGG - tig0025281:3106-3125 Msa1421620:intron 35.0%
!!! ATTTTGGTTCGATTCTTGCA+TGG + tig0025281:3263-3282 None:intergenic 35.0%
ATGCAAGAATCGAACCAAAA+TGG - tig0025281:3262-3281 Msa1421620:CDS 35.0%
TGCAAGAATCGAACCAAAAT+GGG - tig0025281:3263-3282 Msa1421620:CDS 35.0%
CTATGCAGCAGTTATGAAAA+AGG + tig0025281:3371-3390 None:intergenic 35.0%
CTTAATCATTATGATGCCCT+TGG + tig0025281:3415-3434 None:intergenic 35.0%
ACGTTATTCCCTTGATTATG+TGG - tig0025281:3496-3515 Msa1421620:intron 35.0%
CCACAAATTCCACATAATCA+AGG + tig0025281:3508-3527 None:intergenic 35.0%
CCTTGATTATGTGGAATTTG+TGG - tig0025281:3505-3524 Msa1421620:intron 35.0%
! TTTCTTTTGATCAGGTGGTT+AGG - tig0025281:3702-3721 Msa1421620:intron 35.0%
!! GATTTTGAGTCATAGGTCAA+AGG + tig0025281:3866-3885 None:intergenic 35.0%
! GAATTCCATTGTATGCCTTT+TGG - tig0025281:4154-4173 Msa1421620:intron 35.0%
! TAATTTCTGGAGACGTCTTA+TGG - tig0025281:4353-4372 Msa1421620:intron 35.0%
GACTCTGCATAAGACAATAA+TGG - tig0025281:4821-4840 Msa1421620:CDS 35.0%
CAACTAAGAGCTTATCTCAT+TGG + tig0025281:5094-5113 None:intergenic 35.0%
!!! TTTTGGCTTGCAAAATGTGA+TGG - tig0025281:5229-5248 Msa1421620:intron 35.0%
ATAAAAAAGGACGGGGAATG+GGG - tig0025281:710-729 Msa1421620:three_prime_UTR 40.0%
TACAGAAGGTCCATCTAGAT+GGG + tig0025281:1351-1370 None:intergenic 40.0%
TTACAGAAGGTCCATCTAGA+TGG + tig0025281:1352-1371 None:intergenic 40.0%
GAGGAAAGAGAACTTACAGA+AGG + tig0025281:1365-1384 None:intergenic 40.0%
GACATGGTAAGGGTTAGAAA+AGG - tig0025281:1467-1486 Msa1421620:CDS 40.0%
TGGTAAGGGTTAGAAAAGGA+TGG - tig0025281:1471-1490 Msa1421620:CDS 40.0%
!! GCTTTATTTGGGGAAGGTAA+GGG - tig0025281:1573-1592 Msa1421620:CDS 40.0%
GGGAAGGTTGAACTTGATAT+GGG - tig0025281:1630-1649 Msa1421620:intron 40.0%
GGAAGGTTGAACTTGATATG+GGG - tig0025281:1631-1650 Msa1421620:intron 40.0%
AAAACAAAGAGCTACCGTGT+AGG + tig0025281:1675-1694 None:intergenic 40.0%
GCAGAAAAGGCCTTAAAATG+TGG + tig0025281:1704-1723 None:intergenic 40.0%
GCCATATCTGACATACAATC+TGG - tig0025281:1745-1764 Msa1421620:intron 40.0%
GCCAGATTGTATGTCAGATA+TGG + tig0025281:1749-1768 None:intergenic 40.0%
! GAAACTTTTGGCATGTTTGC+AGG - tig0025281:1852-1871 Msa1421620:intron 40.0%
!!! TCTGTTTTTTGTGGAAGAGG+TGG - tig0025281:2054-2073 Msa1421620:intron 40.0%
! TTTTGTGGAAGAGGTGGAAA+TGG - tig0025281:2060-2079 Msa1421620:intron 40.0%
TTCGTTGCAAACTTCAGTTG+GGG - tig0025281:2118-2137 Msa1421620:intron 40.0%
! TTGATGCATCGTTTTGCCTT+TGG - tig0025281:2171-2190 Msa1421620:intron 40.0%
GAGCAGAAACACAAATCCAA+AGG + tig0025281:2190-2209 None:intergenic 40.0%
!! AAGGGATAATTTGCTACCTC+TGG + tig0025281:2270-2289 None:intergenic 40.0%
! GTGAGTGTGTTTGTTACACA+AGG + tig0025281:2377-2396 None:intergenic 40.0%
AGAGAGTCGATTAAAAGGCA+TGG + tig0025281:2479-2498 None:intergenic 40.0%
GAATTCCATTGCATGCTTCT+AGG - tig0025281:2517-2536 Msa1421620:intron 40.0%
GAATTCCATTGTATGCCTCT+TGG - tig0025281:3060-3079 Msa1421620:intron 40.0%
! CTGCTGCATAGATCTTTTTG+TGG - tig0025281:3380-3399 Msa1421620:intron 40.0%
! TTTTGTGGCAGCTAAATCCA+AGG - tig0025281:3395-3414 Msa1421620:intron 40.0%
TTTGTGGCAGCTAAATCCAA+GGG - tig0025281:3396-3415 Msa1421620:intron 40.0%
TCTCGCAACAGTATAAGAGA+CGG - tig0025281:3675-3694 Msa1421620:intron 40.0%
!! CTCAAGCTTGTTGCATATGT+TGG - tig0025281:3726-3745 Msa1421620:intron 40.0%
TGTTAGTGTTGCAAAGTCAG+AGG - tig0025281:3792-3811 Msa1421620:intron 40.0%
GGCTTGCAAGTCCATTATAA+TGG + tig0025281:4080-4099 None:intergenic 40.0%
! GGAATCCAAAAGGCATACAA+TGG + tig0025281:4162-4181 None:intergenic 40.0%
TTTGCGGAATTGTCTGATGA+AGG - tig0025281:4459-4478 Msa1421620:intron 40.0%
ATCACATCTGCAGATTTGTG+TGG + tig0025281:4487-4506 None:intergenic 40.0%
ACAAATCTGCAGATGTGATG+AGG - tig0025281:4488-4507 Msa1421620:intron 40.0%
GTTCCTCCTTCAAGAATTCT+GGG - tig0025281:4968-4987 Msa1421620:intron 40.0%
TTGTCTCCCAGAATTCTTGA+AGG + tig0025281:4977-4996 None:intergenic 40.0%
! AATTCTGGGAGACAAAGAAG+AGG - tig0025281:4982-5001 Msa1421620:intron 40.0%
! AGAGGCATAAAGTTGCATGA+TGG - tig0025281:5000-5019 Msa1421620:intron 40.0%
AGAGAAGAACGCAAAGCCAA+TGG + tig0025281:1043-1062 None:intergenic 45.0%
GTTGAGGATGTTGGCATAGT+TGG - tig0025281:1209-1228 Msa1421620:three_prime_UTR 45.0%
! ATAGTTGGTGCTCCAATGCA+GGG - tig0025281:1224-1243 Msa1421620:three_prime_UTR 45.0%
! GAGTGTACTTTTCCCTGCAT+TGG + tig0025281:1239-1258 None:intergenic 45.0%
AGGAAACCCATTCATGGAGT+GGG - tig0025281:1300-1319 Msa1421620:three_prime_UTR 45.0%
ACCATTTCTCCCCATCTAGA+TGG - tig0025281:1338-1357 Msa1421620:three_prime_UTR 45.0%
TCCATCTAGATGGGGAGAAA+TGG + tig0025281:1342-1361 None:intergenic 45.0%
ACAGAAGGTCCATCTAGATG+GGG + tig0025281:1350-1369 None:intergenic 45.0%
!! GGCTTTATTTGGGGAAGGTA+AGG - tig0025281:1572-1591 Msa1421620:CDS 45.0%
GGGGAAGGTTGAACTTGATA+TGG - tig0025281:1629-1648 Msa1421620:intron 45.0%
GGTTGAACTTGATATGGGGA+AGG - tig0025281:1635-1654 Msa1421620:intron 45.0%
TGTCTTTGTGGAAGTAGTCC+TGG - tig0025281:1934-1953 Msa1421620:intron 45.0%
TCGTTGCAAACTTCAGTTGG+GGG - tig0025281:2119-2138 Msa1421620:intron 45.0%
! TTTTGCTAGTGCAAAGCCAG+AGG - tig0025281:2251-2270 Msa1421620:CDS 45.0%
! CAAGTCCTAGAAGCATGCAA+TGG + tig0025281:2525-2544 None:intergenic 45.0%
AGCTGTCAAAGCTACTGATG+AGG - tig0025281:2740-2759 Msa1421620:intron 45.0%
! AGAGTCCAAGAGGCATACAA+TGG + tig0025281:3068-3087 None:intergenic 45.0%
!!! ACGGCTGGTTTCTTTTGATC+AGG - tig0025281:3694-3713 Msa1421620:intron 45.0%
!! GCTGGTTTCTTTTGATCAGG+TGG - tig0025281:3697-3716 Msa1421620:intron 45.0%
TTCTTGTCGACATACCTGCT+TGG - tig0025281:4421-4440 Msa1421620:intron 45.0%
! AGACAATTCCGCAAAGCGTT+TGG + tig0025281:4454-4473 None:intergenic 45.0%
GGTTCCTCCTTCAAGAATTC+TGG - tig0025281:4967-4986 Msa1421620:intron 45.0%
TCTCCCAGAATTCTTGAAGG+AGG + tig0025281:4974-4993 None:intergenic 45.0%
GCTTGCAAAATGTGATGGCA+AGG - tig0025281:5234-5253 Msa1421620:intron 45.0%
CAAAATGTGATGGCAAGGCA+AGG - tig0025281:5239-5258 Msa1421620:intron 45.0%
AGAAAGAGCCTTGGAGAGAA+AGG - tig0025281:5337-5356 Msa1421620:intron 45.0%
CTGCAGATGCAGATGTTGAT+AGG - tig0025281:5381-5400 Msa1421620:intron 45.0%
!! AATTAAATGAAAAATAAAAA+AGG - tig0025281:697-716 Msa1421620:three_prime_UTR 5.0%
!! AATAAATGAATAAATAATAA+TGG - tig0025281:741-760 Msa1421620:three_prime_UTR 5.0%
!!! TAAGATTTTTTTTTAAAAAA+GGG + tig0025281:2151-2170 None:intergenic 5.0%
!!! ATAAGATTTTTTTTTAAAAA+AGG + tig0025281:2152-2171 None:intergenic 5.0%
!! AATAATAACAATAATAATAT+AGG - tig0025281:4222-4241 Msa1421620:intron 5.0%
!! TAATTATAAATATAATTGTT+AGG - tig0025281:4854-4873 Msa1421620:intron 5.0%
!! AATTATAAATATAATTGTTA+GGG - tig0025281:4855-4874 Msa1421620:intron 5.0%
GAAGCTGTATGGGCCAAAAG+AGG + tig0025281:659-678 None:intergenic 50.0%
! CATAGTTGGTGCTCCAATGC+AGG - tig0025281:1223-1242 Msa1421620:three_prime_UTR 50.0%
CAGGAAACCCATTCATGGAG+TGG - tig0025281:1299-1318 Msa1421620:three_prime_UTR 50.0%
GGAAACCCATTCATGGAGTG+GGG - tig0025281:1301-1320 Msa1421620:three_prime_UTR 50.0%
TCATGCCCCACTCCATGAAT+GGG + tig0025281:1309-1328 None:intergenic 50.0%
ATCATGCCCCACTCCATGAA+TGG + tig0025281:1310-1329 None:intergenic 50.0%
AAAGAGCTACCGTGTAGGTC+AGG + tig0025281:1670-1689 None:intergenic 50.0%
TTTGTGGAAGTAGTCCTGGC+AGG - tig0025281:1938-1957 Msa1421620:intron 50.0%
CGTTGCAAACTTCAGTTGGG+GGG - tig0025281:2120-2139 Msa1421620:intron 50.0%
GCAACAGTATAAGAGACGGC+TGG - tig0025281:3679-3698 Msa1421620:intron 50.0%
GCTGCTATCCAAACGCTTTG+CGG - tig0025281:4443-4462 Msa1421620:intron 50.0%
GCCTTCATACTGCTTTCTGC+TGG - tig0025281:4941-4960 Msa1421620:intron 50.0%
GCCAGCAGAAAGCAGTATGA+AGG + tig0025281:4945-4964 None:intergenic 50.0%
CGAAAGAGCCTTGGAGAGAA+AGG - tig0025281:5292-5311 Msa1421620:intron 50.0%
AGAGCCTTGGAGAGAAAGGA+AGG - tig0025281:5296-5315 Msa1421620:intron 50.0%
GAGCCTTGGAGAGAAAGGAA+GGG - tig0025281:5297-5316 Msa1421620:intron 50.0%
AAGCCCTTCCTTTCTCTCCA+AGG + tig0025281:5303-5322 None:intergenic 50.0%
TTGGAGAGAAAGGAAGGGCT+TGG - tig0025281:5302-5321 Msa1421620:intron 50.0%
GTGAGAGAGAGAAAGAGCCT+TGG - tig0025281:5328-5347 Msa1421620:intron 50.0%
AGAATTTGCAGTGCAGCGCA+GGG + tig0025281:5363-5382 None:intergenic 50.0%
! TTTGGGGTTAGAACCCACGC+TGG - tig0025281:1187-1206 Msa1421620:three_prime_UTR 55.0%
! GTTAGAACCCACGCTGGTTG+AGG - tig0025281:1193-1212 Msa1421620:three_prime_UTR 55.0%
CCAACATCCTCAACCAGCGT+GGG + tig0025281:1203-1222 None:intergenic 55.0%
! CAGGAGAGCACGTGTCTTTG+TGG - tig0025281:1922-1941 Msa1421620:intron 55.0%
TGGAAGTAGTCCTGGCAGGA+AGG - tig0025281:1942-1961 Msa1421620:intron 55.0%
CGTTTGGATAGCAGCCAAGC+AGG + tig0025281:4438-4457 None:intergenic 55.0%
CATACTGCTTTCTGCTGGCG+TGG - tig0025281:4946-4965 Msa1421620:intron 55.0%
TGAGGGAAGCGAAAGAGCCT+TGG - tig0025281:5283-5302 Msa1421620:intron 55.0%
CAGAATTTGCAGTGCAGCGC+AGG + tig0025281:5364-5383 None:intergenic 55.0%
! CCCACGCTGGTTGAGGATGT+TGG - tig0025281:1200-1219 Msa1421620:three_prime_UTR 60.0%
GCCAACATCCTCAACCAGCG+TGG + tig0025281:1204-1223 None:intergenic 60.0%
GGCTACTCTTCCTTCCTGCC+AGG + tig0025281:1955-1974 None:intergenic 60.0%
GCGCAGGGCCTTTCTCTCCA+AGG + tig0025281:5348-5367 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
tig0025281 gene 578 5450 578 ID=Msa1421620;Name=Msa1421620
tig0025281 mRNA 578 5450 578 ID=Msa1421620-mRNA-1;Parent=Msa1421620;Name=Msa1421620-mRNA-1;_AED=0.21;_eAED=0.21;_QI=0|0.4|0.33|0.83|0|0.16|6|863|195
tig0025281 exon 578 1613 578 ID=Msa1421620-mRNA-1:exon:2207;Parent=Msa1421620-mRNA-1
tig0025281 exon 2236 2312 2236 ID=Msa1421620-mRNA-1:exon:2206;Parent=Msa1421620-mRNA-1
tig0025281 exon 3219 3318 3219 ID=Msa1421620-mRNA-1:exon:2205;Parent=Msa1421620-mRNA-1
tig0025281 exon 4018 4103 4018 ID=Msa1421620-mRNA-1:exon:2204;Parent=Msa1421620-mRNA-1
tig0025281 exon 4761 4844 4761 ID=Msa1421620-mRNA-1:exon:2203;Parent=Msa1421620-mRNA-1
tig0025281 exon 5383 5450 5383 ID=Msa1421620-mRNA-1:exon:2202;Parent=Msa1421620-mRNA-1
tig0025281 CDS 5383 5450 5383 ID=Msa1421620-mRNA-1:cds;Parent=Msa1421620-mRNA-1
tig0025281 CDS 4761 4844 4761 ID=Msa1421620-mRNA-1:cds;Parent=Msa1421620-mRNA-1
tig0025281 CDS 4018 4103 4018 ID=Msa1421620-mRNA-1:cds;Parent=Msa1421620-mRNA-1
tig0025281 CDS 3219 3318 3219 ID=Msa1421620-mRNA-1:cds;Parent=Msa1421620-mRNA-1
tig0025281 CDS 2236 2312 2236 ID=Msa1421620-mRNA-1:cds;Parent=Msa1421620-mRNA-1
tig0025281 CDS 1441 1613 1441 ID=Msa1421620-mRNA-1:cds;Parent=Msa1421620-mRNA-1
tig0025281 three_prime_UTR 578 1440 578 ID=Msa1421620-mRNA-1:three_prime_utr;Parent=Msa1421620-mRNA-1
Gene Sequence

>Msa1421620

ATGTCGGGATTTGCGGGAAAGACTTTTCTCTTAGAGTCTGAGATTGAGTTTTGGAACGTCTTGTTTTGGTGTTTGGGGTTAGAACCCACGCTGGTTGAGGATGTTGGCATAGTTGGTGCTCCAATGCAGGGAAAAGTACACTCTTCAGTGTTGAGAGCACGTGTCTTTGTGGAAGTAGTCCTGGCAGGAAGGAAGAGTAGCCATCAAAATGATGCCATTAAACTTGGAAATGAATTAGGTGACACCTTGAGAAACTATGAGATGAAGGAGCTGTCAAAGCTACTGATGAGGACTTGTTTACATGCTTGCTTGCTGCTGTCCAAATACACATTATCATTGTGGTTAGGACTCAAGCTTGTTGCATATGTTGGTATAGTTGATGCTTCAAATGAAGAAAAGCACAATTTTGAGCTTTATCATATTCTTGTCGACATACCTGCTTGGCTGCTATCCAAACGCTTTGCGGAATTGTCTGATGAAGGATCCACACAAATCTGCAGATGTGATGAGGACTTGTTACATGCTTGTTTGCTGCTGCCCATAATACACATTATCACTGTAAGTAAAAAAATGAAAACAATTGTGTAG
Protein sequence

>Msa1421620

MSGFAGKTFLLESEIEFWNVLFWCLGLEPTLVEDVGIVGAPMQGKVHSSVLRARVFVEVVLAGRKSSHQNDAIKLGNELGDTLRNYEMKELSKLLMRTCLHACLLLSKYTLSLWLGLKLVAYVGIVDASNEEKHNFELYHILVDIPAWLLSKRFAELSDEGSTQICRCDEDLLHACLLLPIIHIITVSKKMKTIV*